CN108118062A - Applications of the nitrate anion transporter gene OsNRT1.9a in rice selection and breeding - Google Patents
Applications of the nitrate anion transporter gene OsNRT1.9a in rice selection and breeding Download PDFInfo
- Publication number
- CN108118062A CN108118062A CN201711378092.7A CN201711378092A CN108118062A CN 108118062 A CN108118062 A CN 108118062A CN 201711378092 A CN201711378092 A CN 201711378092A CN 108118062 A CN108118062 A CN 108118062A
- Authority
- CN
- China
- Prior art keywords
- plant
- rice
- genes
- leu
- ser
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
Abstract
The invention discloses applications of the nitrate anion transporter gene OsNRT1.9a in rice selection and breeding, belong to plant genetic engineering field.The amino acid sequence of OsNRT1.9a DNA encoding the protein is as shown in SEQ ID NO.1, and cDNA sequence is as shown in SEQ ID NO.2.By building, rice Os NRT1.9a genes overexpress plant to the present invention, OsNRT1.9a genes disturb plant, it was found that by improving OsNRT1.9a gene expressions, can increase normal rice tillering number, single plant grouting grain number and single plant yield increase, therefore OsNRT1.9a genes can be used in rice selection and breeding improving rice yield.OsNRT1.9a genes have important application value in terms of Nitrogen effect plant growth and growth course is illustrated and in terms of plant type of rice improvement.
Description
Technical field
The invention belongs to plant genetic engineering fields, and in particular to nitrate anion transporter gene OsNRT1.9a is in rice selection and breeding
In application.
Background technology
Rice in China cultivated area accounts for the 20% of the total cultivated area of world crop, but amount of application of nitrogen fertilizer accounts for the world and always applies
The 37% of dosage；Nineteen ninety-five China's nitrogen fertilizer production amount and usage amount have reached No. 1 in the world, but nitrogenous fertilizer service efficiency is relatively low, nitrogenous fertilizer
Amount of application compared with 50 years before increase by 20 times, by this trend, it is contemplated that the year two thousand fifty, it will turn over 3 times again.The excessive meeting of nitrogen application
Cause the ecological pollutions such as body eutrophication problem [Xu Guohua, model know honor rice nitre transporter gene OsNRT1.1a with
The functional study Agricultural University Of Nanjing of OsNRT1.1b, 2011：4-6].More nitrogen nutrition pass through denitrification, water and soil
It is lost in, volatilizees naturally, microorganism such as utilizes to be wasted at the approach.
If the absorption efficiency of nitrogen is improved 1%, it is equivalent to save more than ten hundred million dollars of spending every year.From China
National conditions are analyzed, and the potentiality that expansion cultivated area is increased the total yield are very limited, and the only way out is raw on limited soil
The more paddy of output, that is, increase the yield per unit area.In traditions of the past farming, by selecting nitrogen use efficiency higher
Crop, to improve the utilization ratio of nitrogen；But compared with the breeding on molecular level, this process seems slow and poorly efficient [Zhang Hong
Journey, wears genotypic difference and physiological Mechanism research Yangzhou Universitys that its root Nitrogen in Rice utilizes, and 2008：10-13].It improves
Nitrogen use efficiency, it is necessary to find breach from the molecule absorption mechanism of nitrogen.Nitrate anion transporter gene family is divided into low-affinity nitre
[Zhou Shiyi carbohydrates and amino acid are to the high parent of rice induction type with two class of high-affinity nitrate anion transporter gene for acid group transporter gene
With the influence Central China University of Science and Technology of power nitrate transport system, 2009：15-16].By nitrogen assimilation, by nitrate nitrogen and ammonium
State nitrogen absorbs and is converted into amino acid, and the first kind for being known as nitrogen absorbs.By increasing seed nutritional substance to the transport of nitrogen, increase
Add plumpness, the second class for being known as nitrogen absorbs, that is, recycling [Kant S, Bi Y, the Steven J, et of nitrogen
al.Understanding plant response to nitrogen limitation for the improvement of
crop nitrogen use efficiency.Journal of Experimental,2011,62(4):1499-1509].Increase
Add nitrogen absorption and accumulation amount or N transformation amount, can increase production.Therefore, in agricultural modernization construction, molecular breeding means are passed through
To improve utilization ratio of the rice to nitrogenous fertilizer, it is possible to reduce nitrogenous fertilizer pollutes, moreover it is possible to increase yield.
NRT1/PTR families (NRT1/PTR family, NPF) are the small molecules for referring to 2-3 amino acid residue of mediation
The substances such as peptide and nitrate anion carry out albumen [Rentsch D, Schmidt S, the Tegeder M.Transporters of transdermal delivery
for uptake and allocation of organic nitrogen compounds in plants.FEBS Let,
2007,581:2281-2289].NRT1/PTR family members are taken part in during Seed Development in the accumulation and sprouting of protein
Micromolecule polypeptide form transports [Martre P, Porter J R, Jamieson P D, et al.Modeling after protein degradation
grain nitrogen accumulation and protein composition to understand the sink/
source regulations of nitrogen remobilization for wheat.Plant Physiol,2003,
133:1959-1967].Report at present on NPF family members research is seldom, and OsNRT1.9 genes of the present invention are water
One nitrate anion transporter gene of rice NPF gene families.Present invention discover that OsNRT1.9 genetic transcriptions post-processing can form two kinds
Montage, wherein the first splicing form OsNRT1.9a have rice tillering extremely important effect, can be applied to plant plant type and change
It is good so that increasing production of rice.
The content of the invention
It is an object of the invention to solve problems of the prior art, rice NPF gene family member's nitric acid is provided
Applications of the root transporter gene OsNRT1.9a in rice selection and breeding.
The purpose of the present invention is achieved through the following technical solutions：
The present invention is using the NPF gene family members nitrate anion transporter gene OsNRT1.9a of rice as object, the flower from rice
The cDNA sequence of OsNRT1.9a has been cloned in 11.By building OsNRT1.9a gene overexpression vectors, using Agrobacterium
The genetic transforming method of EHA105 mediations, overexpression vector is imported in normal japonica rice variety in spending 11, obtains OsNRT1.9a bases
Because overexpressing plant, tiller number, number of productive ear, grouting kernal number and yield significantly carry compared with compareing and spending 11 in wild type
It is high.OsNRT1.9a gene interference expression vectors are built by RNAi technology, in spending 11 during interference expression vector is imported, are obtained
The interference plant that OsNRT1.9a gene expression amounts decline disturbs tiller number, number of productive ear, grouting kernal number and the yield of plant
It is significantly reduced compared with spending 11 in.These results indicate that the expression by improving OsNRT1.9a genes, can make normal water
Rice tiller number increases, so as to improve spike number, grouting kernal number and rice yield.
Based on present invention discover that OsNRT1.9a genes function, OsNRT1.9a genes can be used for rice selection and breeding in.Institute
The rice selection and breeding stated is improve rice tillering number, so as to improve spike number, grouting kernal number and rice yield.Raising can specifically be passed through
The expression of OsNRT1.9a genes makes rice tillering number and panicle number per hill increases, be in the milk kernal number, reaches the mesh for improving rice yield
's.
OsNRT1.9a genes can also be used for improving the yield of other plant, such as by transgenosis OsNRT1.9a genes be made to exist
(excess) is expressed in plant, to improve the branch quantity of plant, and then is improved the yield of plant.The plant refers to
Monocotyledon or dicotyledon；Such as：Wheat, tomato, turfgrass or clover etc..
The amino acid sequence of the OsNRT1.9a albumen of the OsNRT1.9a gene codes is as shown in SEQ ID NO.1；
The cDNA sequence of the OsNRT1.9a genes is preferably as shown in SEQ ID NO.2.
It is construed as, (i.e. not in the activated centre of albumen) on the premise of the white activity of OsNRT1.9a is not influenced, ability
It is one or several that field technique personnel can carry out various substitutions, additions and/or deletions to the amino acid sequence shown in SEQ ID NO.1
Amino acid acquisition has the function of equal amino acid sequence.Therefore, OsNRT1.9a albumen further includes ammonia shown in SEQ ID NO.1
Base acid sequence is substituted, replaces and/or increases the protein having with isoreactivity that one or several amino acid obtain.In addition,
It is to be understood that the preferences of the degeneracy and different plant species codon in view of codon, those skilled in the art can basis
It needs using the codon that particular species is suitble to express.
Advantages of the present invention and effect：
(1) enhance Tillering Ability in Rice after the OsNRT1.9a genes overexpression that the present invention clones, illustrate OsNRT1.9a
Gene pairs raising rice yield is more apparent, and therefore, the expression of OsNRT1.9a genes is improved by technique for gene engineering to improve
Plant products.It is not only does this facilitate and cultivates high-yield rice under the conditions of nitrogen by normally applying, can also be planted by molecular breeding
The breed improvement of object.
(2) successful clone of OsNRT1.9a genes further demonstrates important work of the NPF families in nitrogen absorption process
With, there is important meaning to the biological function for illustrating NPF families, in addition to further appreciating that plant nitrogen metabolism approach, raising
Nitrogen absorption efficiency has great impetus.
(3) although being cloned into some genes for improving plant products at present, to the molecular mechanism of plant yield-increasing still
It is unclear.And the OsNRT1.9a genes that the present invention clones can improve the yield of rice, to determining the key factor of plant yield-increasing
There is great impetus.
Description of the drawings
Fig. 1 is the whole strain phenotypic map that 11 and OsNRT1.9a genes overexpression 3 strains of plant are spent in control.
Fig. 2 is the statistics block diagram that 11 and OsNRT1.9a genes overexpression 3 strain tiller numbers of plant are spent in control, is counted
Variable analysis (ANOVA) is carried out according to using SPSS softwares, uses Duncan ' s enterprising in 0.05,0.01 and 0.001 3 level
Row significance difference analysis is represented compared with the control with *, * * and * * * respectively.
Fig. 3 is the whole strain phenotypic map that 11 and OsNRT1.9a genes interference 3 strains of plant are spent in control.
Fig. 4 be control in spend 11 and OsNRT1.9a genes interference 3 strain tiller numbers of plant statistics block diagram, data
Variable analysis (ANOVA) is carried out using SPSS softwares, Duncan ' s is used to be carried out in 0.05,0.01 and 0.001 3 level
Significance difference analysis is represented compared with the control with *, * * and * * * respectively.
Fig. 5 is that 11, OsNRT1.9a genes overexpression 3 strains of plant and OsNRT1.9a genes interference plant are spent in control
The OsNRT1.9a gene expression amount testing result figures of 3 strains, data carry out variable analysis (ANOVA) using SPSS softwares, make
Significance difference analysis is carried out in 0.05,0.01 and 0.001 3 level with Duncan ' s, uses *, * * respectively compared with the control
It is represented with * * *.
Fig. 6 is that 11, OsNRT1.9a genes overexpression 3 strains of plant and OsNRT1.9a genes interference plant are spent in control
Every plant of grouting seed phenotypic map of 3 strains.
Fig. 7 is that 11, OsNRT1.9a genes overexpression 3 strains of plant and OsNRT1.9a genes interference plant are spent in control
Every plant of grouting seed quantity statistical chart of 3 strains.Data carry out variable analysis (ANOVA) using SPSS softwares, use
Duncan ' s carry out significance difference analysis in 0.05,0.01 and 0.001 3 level, use *, * * respectively compared with the control
It is represented with * * *.
Fig. 8 is that 11, OsNRT1.9a genes overexpression 3 strains of plant and OsNRT1.9a genes interference plant are spent in control
Every plant of output statistics figure of 3 strains, data carry out variable analysis (ANOVA) using SPSS softwares, Duncan ' s are used to exist
0.05th, significance difference analysis is carried out in 0.01 and 0.001 3 level, is represented respectively with *, * * and * * * compared with the control.
Specific embodiment
With reference to embodiment, the present invention will be further described in detail, but the implementation of the present invention is not limited to this.
Unless otherwise specified, the conventional means that the technological means used in following embodiments is well known to those skilled in the art；Used
Experimental method is conventional method, and can according to described recombinant technique (referring to molecular cloning, laboratory manual, second edition,
CSH Press, Cold SpringHarbor, New York) it completes；Material, reagent used etc., are commercially available.
1 OsNRT1.9a genes of embodiment overexpress the structure of plant
11 RNA is spent in extraction rice, and its reverse transcription is utilized into primer pair into cDNA：
F1：5'-AGATCTATGGCCGCCATAGAAGAGGAG-3'(Bgl II),
R1：5'-CTTAAGTCATGAAGCTGTGTTCTCTCT-3'(Afl II)；
After cDNA by PCR amplification OsNRT1.9a genes, by being connected into pCAMBIA- after Bgl II, Afl II digestions
1301 carriers (pCAMBIA-1301 carriers are purchased from Cambia companies) construct the overexpression vector of OsNRT1.9a genes
OsNRT1.9a-p1301.The genetic transforming method mediated using Agrobacterium EHA105, normal rice product are imported by overexpression vector
It is spent in kind in 11.
It by the transplanting of obtained all transgenic plants in the basket with soil, periodically waters, fertilising treats that seedling grows tall about
During 10cm, plant in big Tanaka, after seedling is grown up, extraction genomic DNA is detected transfer-gen plant by PCR, and detection is drawn
Object to for：
F2：5'-GATGTTGGCGACCTCGTATT-3',
R2：5'-TCGTTATGTTTATCGGCACTTT-3'.
If amplifying the segment of 517bp, it is positive plant to illustrate transfer-gen plant.Positive plant single plant sowing is simultaneously planted
It plants, until T2 generations identify homozygous transfer-gen plant overexpresses plant to get to OsNRT1.9a genes.OsNRT1.9a genes
The tiller number of plant is overexpressed far more than spending 11 plant, significant difference, as shown in Fig. 1,2 in control.Detection overexpression plant
The expression quantity of OsNRT1.9a genes, the expression of display OsNRT1.9a genes are improved compared with the control, as shown in Figure 5.It is single
Seed statistics is collected in strain, the results showed that every plant of grouting seed of overexpression plant increases, and every plant of yield increases, such as the institute of Fig. 6,7,8
Show.
2 OsNRT1.9a genes of embodiment disturb the acquisition of plant
11 RNA is spent in extraction rice, and its reverse transcription is utilized into primer pair into cDNA：
F3：5'-GGTACCAGCCAGCCCTGAAGCACAGCAC-3'(Kpn I),
R3：5'-GGATCCTGCACCACTCCCAAGGGCAGCA-3'(BamH I)；
F4：5'-ACTAGTAGCCAGCCCTGAAGCACAGCAC-3'(Spe I),
R4：5'-GAGCTCTGCACCACTCCCAAGGGCAGCA-3'(Sac I)；
After respective PCR amplification goes out the cDNA segments of OsNRT1.9a genes, by connecting after corresponding digestion with restriction enzyme
Enter pTCK303 carriers, construct the interference expression vector OsNRT1.9a-pTCK303 of OsNRT1.9a genes.Using Agrobacterium
The genetic transforming method of EHA105 mediations, interference expression vector is imported in normal japonica rice variety and is spent in 11.
It by the transplanting of obtained all transgenic plants in the basket with soil, periodically waters, fertilising treats that seedling grows tall about
During 10cm, plant in big Tanaka, after seedling is grown up, extraction genomic DNA is detected transfer-gen plant by PCR, and detection is drawn
Object to for：
F2：5'-GATGTTGGCGACCTCGTATT-3',
R2：5'-TCGTTATGTTTATCGGCACTTT-3'.
If amplifying the segment of 517bp, it is positive plant to illustrate transfer-gen plant.Positive plant single plant sowing is simultaneously planted
It plants, until T2 generations identify homozygous transfer-gen plant disturbs plant to get to OsNRT1.9a genes.OsNRT1.9a genes are done
The tiller number of plant is disturbed far fewer than spending 11 plant, significant difference, as shown in Fig. 3,4 in control.Detection interference plant
The expression quantity of OsNRT1.9a genes, the expression of display OsNRT1.9a genes are reduced compared with the control, as shown in Figure 5.It is single
Seed statistics is collected in strain, the results showed that every plant of grouting seed of interference plant is reduced, and every plant of yield is reduced, as shown in Fig. 6,7,8.
The above results show the expression by improving OsNRT1.9a genes, can increase the tiller number of rice, Jin Erti
High spike number and rice yield.
Above-described embodiment is the preferable embodiment of the present invention, but embodiments of the present invention and from above-described embodiment
Limitation, other any Spirit Essences without departing from the present invention with made under principle change, modification, replacement, combine, simplification,
Equivalent substitute mode is should be, is included within protection scope of the present invention.
Sequence table
<110>Wuhan Bioengineering Institute
<120>Applications of the nitrate anion transporter gene OsNRT1.9a in rice selection and breeding
<160> 2
<170> SIPOSequenceListing 1.0
<210> 1
<211> 576
<212> PRT
<213> Oryza sativa
<400> 1
Met Ala Ala Ile Glu Glu Glu Arg Pro Leu Leu Pro Leu Gln Ser Gln
1 5 10 15
Asp Val Gly Ser Glu Tyr Thr Arg Asp Gly Ser Val Asp Ile Asn Lys
20 25 30
Glu Pro Ala Leu Lys His Ser Thr Gly Asn Trp Arg Ala Cys Phe Leu
35 40 45
Ile Leu Gly Val Glu Phe Cys Glu Asn Met Thr Tyr Phe Val Ile Ser
50 55 60
Arg Asn Leu Val Thr Phe Leu Thr Thr Val Leu His Glu Ser Lys Val
65 70 75 80
Asp Ala Ala Arg Asn Val Ser Ala Trp Val Gly Ala Cys Phe Leu Thr
85 90 95
Pro Val Val Gly Ala Phe Leu Ala Asp Thr Tyr Trp Gly Arg Tyr Trp
100 105 110
Thr Ile Val Val Phe Leu Pro Val Tyr Ile Thr Gly Met Leu Ile Val
115 120 125
Thr Val Ser Ala Ser Leu Pro Met Phe Leu Thr Ser Ser Glu His Gly
130 135 140
Asn Val His Arg Ser Val Val Tyr Leu Gly Leu Tyr Leu Ala Ala Leu
145 150 155 160
Gly Ser Gly Ala Met Lys Pro Cys Thr Ser Ser Phe Gly Ala Asp Gln
165 170 175
Phe Asp Ser Thr Asp Leu Glu Glu Leu Pro Lys Lys Ala Ser Phe Phe
180 185 190
Ser Trp Ser Phe Tyr Met Thr Thr Val Ser Thr Leu Leu Ser Ser Thr
195 200 205
Val Leu Val Trp Leu Gln Asp Asn Val Gly Trp Gly Val Gly Cys Ala
210 215 220
Ile Pro Thr Val Phe Met Ile Ile Ser Phe Pro Val Phe Ile Ala Gly
225 230 235 240
Ser Arg Val Tyr Arg Phe Arg Asn Leu Gly Phe Ser Pro Leu Lys Ser
245 250 255
Leu Cys Gln Val Ile Val Ala Ala Val Arg Lys Cys His Leu Gln Leu
260 265 270
Pro Glu Asn Lys Ser Leu Leu Tyr Glu Pro Ser Asn Ser Ser Ser Thr
275 280 285
Thr Glu Ala Ser His Lys Ile Gln Pro Thr Asn Gln Phe Arg Phe Leu
290 295 300
Asp Lys Ala Ala Ile Val Leu Pro Pro Ser Asp Glu Thr Cys Ile Lys
305 310 315 320
Pro Met Ser Ser Trp Ser Leu Cys Thr Val Thr Gln Val Glu Glu Leu
325 330 335
Lys Met Leu Leu Arg Met Phe Pro Thr Trp Ala Ser Phe Val Ile Phe
340 345 350
Phe Ala Val Asn Gly Gln Met Ser Ser Thr Phe Ile Glu Gln Gly Met
355 360 365
Ala Met Asp Asn His Val Gly Ser Phe Ala Ile Pro Pro Ala Ser Leu
370 375 380
Thr Ile Ile Ala Val Leu Ser Val Leu Val Leu Val Pro Val Tyr Glu
385 390 395 400
Ile Ile Ser Val Pro Leu Val Lys His Phe Thr Gly Gln Asp Lys Gly
405 410 415
Phe Ser His Ala Gln Arg Ile Gly Ile Gly Leu Ser Leu Ser Met Ile
420 425 430
Met Met Val Tyr Ala Ala Leu Leu Glu Met Lys Arg Leu Ala Ile Val
435 440 445
Gln Ser Ser Gly Leu Ala Asp His Asn Val Ala Ala Pro Met Ser Ile
450 455 460
Leu Trp Gln Thr Pro Ala Tyr Phe Leu Gln Gly Val Ser Glu Ile Phe
465 470 475 480
Ser Cys Ile Gly Met Ser Gln Phe Phe Tyr Asp Gln Ala Pro Asp Ser
485 490 495
Met Lys Ser Val Cys Ala Ala Leu Gly Gln Leu Ala Ile Ala Ser Gly
500 505 510
Ala Tyr Phe Asn Thr Phe Val Leu Gly Ala Val Ala Val Ile Thr Thr
515 520 525
Ser Ser Gly Ala Pro Gly Trp Ile Pro Asp Asn Leu Asn Glu Gly His
530 535 540
Leu Asp Tyr Phe Phe Trp Met Met Ala Thr Leu Ser Leu Leu Asn Leu
545 550 555 560
Ala Met Phe Val Tyr Ser Ser Thr Arg His Arg Glu Asn Thr Ala Ser
565 570 575
<210> 2
<211> 1731
<212> DNA
<213> Oryza sativa
<400> 2
atggccgcca tagaagagga gaggcctctg cttcctctcc aatctcagga tgtaggttca 60
gaatacacaa gggatggttc agttgacatc aacaaggagc cagccctgaa gcacagcaca 120
gggaactgga gggcgtgctt cttgatttta ggtgttgaat tttgtgaaaa catgacctac 180
tttgtaatct cgaggaatct agtcacattc ctcaccactg tgctccacga aagcaaggtc 240
gatgctgcca gaaatgtctc tgcctgggtt ggagcttgct tcctcacacc ggttgttggt 300
gcctttctgg cagacactta ttggggcaga tactggacaa ttgttgtttt cctcccggtg 360
tacatcactg gaatgctcat cgtgacagtt tcagcatcac ttccaatgtt cttgacatct 420
tctgaacatg gcaatgttca tcgttccgta gtgtatctag ggctctatct tgctgccctt 480
gggagtggtg caatgaaacc atgcacttca tcctttgggg ccgaccagtt tgatagcact 540
gatctggagg agttaccgaa gaaggcctcc ttcttcagtt ggtccttcta catgactact 600
gtcagcacct tgctgtcaag cacagtgctt gtttggttgc aagacaatgt tggatggggg 660
gtgggttgcg caatcccgac tgtgttcatg atcatcagtt tccctgtatt cattgccggc 720
tcaagagttt acaggtttag gaacctggga tttagccccc tcaagagcct ctgtcaggtg 780
attgttgcag ctgttaggaa gtgccatctg caattgccag aaaataagtc acttttatat 840
gagccatcca attcatcttc aacaactgaa gcaagtcata aaattcagcc caccaatcaa 900
ttcaggttcc ttgacaaggc agccattgta ctgcccccat cagacgaaac gtgcatcaag 960
cccatgagct catggtcgct ctgcacagtg acacaagttg aggagctgaa gatgctgctg 1020
cggatgttcc ccacctgggc atctttcgtg atctttttcg cggtcaatgg gcagatgtcc 1080
tcaacgttca ttgagcaggg aatggccatg gacaaccatg ttggttcatt tgcaatccca 1140
cctgcatccc tcaccatcat cgccgtgctc agcgtccttg tcttggttcc tgtgtatgag 1200
atcatatcag tgccactggt gaagcatttc accggacagg acaaaggctt ctcacatgcg 1260
cagcgcatcg gaatcggcct ttcactgtcc atgatcatga tggtgtacgc agcattgctc 1320
gagatgaagc ggctggcaat cgtgcaatca agtggcttag cagaccacaa tgtagctgct 1380
ccaatgagta tcctgtggca gacaccagca tactttctgc aaggggtttc agagattttc 1440
agctgcatcg gtatgtcaca gttcttctat gaccaggcgc cagacagcat gaagagcgtt 1500
tgcgcagcgc ttgggcagct cgcaatcgct tcaggagctt acttcaacac gttcgtgctt 1560
ggcgctgtcg cagtgatcac gacgagcagt ggagcacctg ggtggattcc ggataatctg 1620
aatgagggcc atctggacta tttcttctgg atgatggcca cccttagctt acttaatctt 1680
gccatgtttg tgtattcttc cacgaggcac agagagaaca cagcttcatg a 1731
Claims (10)
1.OsNRT1.9a application of the gene in rice selection and breeding, it is characterised in that：The rice selection and breeding are raising rice tillering
Number.
2.OsNRT1.9a application of the gene in rice spike number is improved.
3.OsNRT1.9a application of the gene in rice milking stage grain number is improved.
4.OsNRT1.9a is because of the application in rice yield is improved.
Application of the 5.OsNRT1.9a genes in plant products are improved.
6. according to claim 1-5 any one of them applications, it is characterised in that：By the expression for improving OsNRT1.9a genes
Realize the application.
7. application according to claim 5, it is characterised in that：The plant refers to monocotyledon or dicotyledon.
8. the application according to claim 5 or 7, it is characterised in that：The plant include wheat, tomato, turfgrass or
Clover.
9. according to claim 1-5 any one of them applications, it is characterised in that：The OsNRT1.9a gene codes
The amino acid sequence of OsNRT1.9a albumen is as shown in SEQ ID NO.1；Or OsNRT1.9a albumen is ammonia shown in SEQ ID NO.1
Base acid sequence is substituted, replaces and/or increases the protein having with isoreactivity that one or several amino acid obtain.
10. application according to claim 9, it is characterised in that：The cDNA sequence such as SEQ of the OsNRT1.9a genes
Shown in ID NO.2.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711378092.7A CN108118062B (en) | 2017-12-19 | 2017-12-19 | Application of nitrate transport gene OsNRT1.9a in rice breeding |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711378092.7A CN108118062B (en) | 2017-12-19 | 2017-12-19 | Application of nitrate transport gene OsNRT1.9a in rice breeding |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN108118062A true CN108118062A (en) | 2018-06-05 |
| CN108118062B CN108118062B (en) | 2020-05-29 |
Family
ID=62229545
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711378092.7A Active CN108118062B (en) | 2017-12-19 | 2017-12-19 | Application of nitrate transport gene OsNRT1.9a in rice breeding |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108118062B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110982828A (en) * | 2020-01-02 | 2020-04-10 | 南京农业大学 | Nitrate transport protein gene specifically induced by rice arbuscular mycorrhiza and application thereof |
Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101027397A (en) * | 2004-04-23 | 2007-08-29 | 西尔斯公司 | Nucleotide sequences and polypeptides encoded thereby useful for modifying nitrogen use efficiency characteristics in plants |
| CN101535483A (en) * | 2004-12-16 | 2009-09-16 | 赛乐斯股份有限公司 | Modulating plant nitrogen levels |
| CN106119262A (en) * | 2016-07-28 | 2016-11-16 | 武汉生物工程学院 | Improve Oryza sativa L. nitrogen use efficiency and the gene OsPTR10 of yield and purposes |
| CN106222180A (en) * | 2016-07-28 | 2016-12-14 | 武汉生物工程学院 | Improve rice yield and the gene OsNPF7.3 of grain of rice protein content and purposes |
| CN106337055A (en) * | 2016-10-25 | 2017-01-18 | 武汉生物工程学院 | Application of nitrate radical transporter gene OsNRT1.8 in rice breeding |
| CN106967730A (en) * | 2017-05-19 | 2017-07-21 | 武汉生物工程学院 | Application of the OsNPF6.3 genes in rice tillering number is improved |
| CN107056909A (en) * | 2017-05-23 | 2017-08-18 | 武汉生物工程学院 | Application of the OsNPF5.11 genes in rice yield is improved |
| CN107099549A (en) * | 2017-05-16 | 2017-08-29 | 武汉生物工程学院 | Application of the OsNPF5.16 genes in paddy rice single plant yield is improved |
-
2017
- 2017-12-19 CN CN201711378092.7A patent/CN108118062B/en active Active
Patent Citations (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101027397A (en) * | 2004-04-23 | 2007-08-29 | 西尔斯公司 | Nucleotide sequences and polypeptides encoded thereby useful for modifying nitrogen use efficiency characteristics in plants |
| CN101535483A (en) * | 2004-12-16 | 2009-09-16 | 赛乐斯股份有限公司 | Modulating plant nitrogen levels |
| CN106119262A (en) * | 2016-07-28 | 2016-11-16 | 武汉生物工程学院 | Improve Oryza sativa L. nitrogen use efficiency and the gene OsPTR10 of yield and purposes |
| CN106222180A (en) * | 2016-07-28 | 2016-12-14 | 武汉生物工程学院 | Improve rice yield and the gene OsNPF7.3 of grain of rice protein content and purposes |
| CN106337055A (en) * | 2016-10-25 | 2017-01-18 | 武汉生物工程学院 | Application of nitrate radical transporter gene OsNRT1.8 in rice breeding |
| CN107099549A (en) * | 2017-05-16 | 2017-08-29 | 武汉生物工程学院 | Application of the OsNPF5.16 genes in paddy rice single plant yield is improved |
| CN106967730A (en) * | 2017-05-19 | 2017-07-21 | 武汉生物工程学院 | Application of the OsNPF6.3 genes in rice tillering number is improved |
| CN107056909A (en) * | 2017-05-23 | 2017-08-18 | 武汉生物工程学院 | Application of the OsNPF5.11 genes in rice yield is improved |
Non-Patent Citations (4)
| Title |
|---|
| HSU PK ET AL: "Two phloem nitrate transporters, NRT1.11 and NRT1.12, are important for redistributing xylem-borne nitrate to enhance plant growth", 《PLANT PHYSIOL》 * |
| WANG YY ET AL: "Arabidopsis nitrate transporter NRT1.9 is important in phloem nitrate transport", 《PLANT CELL》 * |
| 无: "ACCESSION NO. XM_015757658，PREDICTED: Oryza sativa Japonica Group protein NRT1/ PTR FAMILY 8.3 (LOC4347971),mRNA", 《GENBANK》 * |
| 蔡昭艳 等: "植物寡肽运输与硝酸根运输基因家族的研究进展", 《热带亚热带植物学报》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110982828A (en) * | 2020-01-02 | 2020-04-10 | 南京农业大学 | Nitrate transport protein gene specifically induced by rice arbuscular mycorrhiza and application thereof |
| CN110982828B (en) * | 2020-01-02 | 2022-08-30 | 南京农业大学 | Nitrate transport protein gene specifically induced by rice arbuscular mycorrhiza and application thereof |
Also Published As
| Publication number | Publication date |
|---|---|
| CN108118062B (en) | 2020-05-29 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN106337055B (en) | Application of the nitrate anion transporter gene OsNRT1.8 in rice breeding | |
| CN106119262B (en) | Improve the gene OsPTR10 and purposes of rice nitrogen use efficiency and yield | |
| Perchlik et al. | Improving plant nitrogen use efficiency through alteration of amino acid transport processes | |
| CN108034672A (en) | Applications of the nitrate anion transporter gene OsNRT1.9b in rice selection and breeding | |
| Anbessa et al. | Strategies to increase nitrogen use efficiency of spring barley | |
| CN106222180B (en) | Improve the gene OsNPF7.3 and purposes of rice yield and grain of rice protein content | |
| CN106518993B (en) | Application of the amino acid transport gene OsAAP3 in rice breeding | |
| CN106929522B (en) | Amino acid transport gene OsAAP1 promotes the application of paddy growth under low nitrogen | |
| CN107099549A (en) | Application of the OsNPF5.16 genes in paddy rice single plant yield is improved | |
| CN106434693A (en) | Application of amino acid transport gene OsAAP4 to rice breeding | |
| CN107987140B (en) | Application of gibberellin transport gene OsNPF3.1 in increasing rice yield | |
| CN106967730A (en) | Application of the OsNPF6.3 genes in rice tillering number is improved | |
| CN106868022B (en) | Nitrogen transport gene OsNPF2.4b for promoting increase of effective spike number of rice and application thereof | |
| CN103012574A (en) | Low-phosphor stress response regulatory factor ZmPHR1, gene for coding the protein and application | |
| CN108070601A (en) | Application of the OsNPF8.6b genes in rice yield is improved | |
| CN108118062A (en) | Applications of the nitrate anion transporter gene OsNRT1.9a in rice selection and breeding | |
| CN107012153A (en) | Applications of the nitrogen nutrition transporter gene OsNPF8.1 in rice tillering number is improved | |
| CN108034661A (en) | Application of the OsNPF8.8b genes in rice yield and nutritional quality is improved | |
| CN105198967A (en) | Application of protein VdAL to improvement of plant product quality | |
| CN107056909A (en) | Application of the OsNPF5.11 genes in rice yield is improved | |
| CN107936103A (en) | Application of the OsNPF7.11b genes in rice yield is improved | |
| CN109207452A (en) | Sorghum resistance related gene SbERECTA and its coding albumen and application | |
| CN106967745B (en) | Application of nitrogen nutrition transport gene OsNPF7.1 in improving tillering and spike number of rice | |
| Egle et al. | Assessing post‐anthesis nitrogen uptake, distribution and utilisation in grain protein synthesis in barley (Hordeum vulgare L.) using 15N fertiliser and 15N proteinogenic and non‐proteinogenic amino acids | |
| CN106947777B (en) | Application of nitrogen transport gene OsNPF7.4 in rice breeding |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |