CN108118013B - One plant for purifying bacterial strain and its application of molasses alcohol waste water - Google Patents
One plant for purifying bacterial strain and its application of molasses alcohol waste water Download PDFInfo
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- CN108118013B CN108118013B CN201810068347.8A CN201810068347A CN108118013B CN 108118013 B CN108118013 B CN 108118013B CN 201810068347 A CN201810068347 A CN 201810068347A CN 108118013 B CN108118013 B CN 108118013B
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
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- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/34—Nature of the water, waste water, sewage or sludge to be treated from industrial activities not provided for in groups C02F2103/12 - C02F2103/32
- C02F2103/36—Nature of the water, waste water, sewage or sludge to be treated from industrial activities not provided for in groups C02F2103/12 - C02F2103/32 from the manufacture of organic compounds
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Abstract
The present invention relates to one plant of microbial strains and its applications, and in particular to a kind of lysine bacillus (Lysinibacillus sp.) S6 and its application in molasses alcohol waste water belong to technical field of microbe application.The object of the present invention is to provide one kind the bacterial strain be can be in microbial strains lysine bacillus (Lysinibacillus sp.) S6 grown using molasses alcohol waste water as sole carbon source, and its corresponding condition of culture and in the case where being disclosed in and not adding any microelement, COD removal effect is ideal, and 48h is up to 64.22%.In addition, the bacterium also has certain denitrification dephosphorization function.
Description
Technical field
The invention belongs to microorganism field, in particular to one plant for purifying bacterial strain and its application of molasses alcohol waste water.
Background technique
Sugaring by-product-molasses, are usually used to fermenting and producing alcohol, can release a large amount of height during producing alcohol
The organic wastewater of concentration, referred to as molasses alcohol waste water.A large amount of waste water will cause water eutrophication without processing direct emission
Change, destroys water body and terrestrial ecosystems.Molasses alcohol waste water is directly used in agriculture filling, will cause soil acidification hardened phenomenon.Such as
Fruit is used to pour sugarcane field, sugarcane yield can be made to increase, but can reduce the sugar content in sugarcane.Currently processed molasses alcohol
The method rationalization method and bioremediation of water.It is compared to physico-chemical method, bioremediation has more safety high
Effect, the period is short, at low cost, not will cause secondary pollution, can also generate biogas energy during processing, treated thallus
It is good forage protein again.These advantages make key points and difficulties of the bioremediation as current research.For biology
For processing method, good microorganism fungus kind is to guarantee that biological wastewater treatment is successfully crucial, is screened from environment excellent
Microbial resources are a kind of important channels.So recent researches person, which will filter out, has efficiently life to molasses alcohol waste water
Starting point of the microorganism (bacterium, fungi etc.) of object degradation capability as research.Wang Weijia is from molasses alcohol oxidation pond
Isolated two plants of bacteriums PSB-C and PSB-D, this two plants of bacteriums belong to the Rhodopseudomonas of photosynthetic bacteria.In optimum response
Under the conditions of (COD concentration is 20800mg/L, and reaction temperature is 30 DEG C, reaction time 3d) two plants of bacterium to the COD removal rate point of waste water
It is not 55% and 54%.Liu Jianfu filters out 8 plants of bacteriums, respectively skin Bacillus, stick from the reactor granules sludge of UASB
Bacillus, Exiguobacterium sp category, lactobacillus, Cellulomonas, Propionibacterium, red long life Pseudomonas are in waste water COD concentration
24000mg/L is handled under the conditions of 37 DEG C, and the removal rate of COD is 25.1% (query: to see document composite bacteria agent COD removal rate 90%
More than, it is therefore desirable to documentation & info is provided, information is to deserved more more clear).The flora that Fan Yanxia is enriched with from IC reactor sludge,
The distribution of bacterium belong to Erysipelotrichales, Clostridiales, Lactobacillales, Xanthomonadales,
In 7 mesh of Burkholderiales, Enterobacteriales, Bacteroidales, under optimum reaction conditions, simultaneously
Nutriment is added, 7d is handled, the removal rate of COD is 38.5%.Mohana, which screens to obtain flora DMC, to be inoculated into while adding Portugal
In grape sugar and inorganic salts molasses alcohol waste water, 37 DEG C of processing 4d, COD removal rate is 51%, decolorization rate of wastewater 67%；The flora
It is compared through 16SrDNA, including pseudomonas aeruginosa (Pseudomonas aeruginosa PAO1), thermophilic malt Flavobacterium
(stenotrophomonas maltophila), proteus mirabilis (Proteus mirabilis).Chopra white rot is true
Bacterium and manyzoned polypore bacteria (C.versicolor) handle molasses alcohol waste water, add glucose and peptone as nutriment, after 8d
COD and the removal rate of coloration are all 53%.Ghosh is with pseudomonas putida (Pseudomonas putida) and Aeromonas
(Aeromonas sp.) two-step pretreatment molasses alcohol waste water adds 1% glucose in waste water.Finally, it is disliked after handling for 24 hours
The COD removal rate of smelly pseudomonad is 44.4%, chroma removal rate 60%, and the COD removal rate of Aeromonas is 44.4%.
Currently, the report of molasses alcohol waste water COD is reduced using lysine bacillus not yet.
Summary of the invention
The primary purpose of the present invention is that the shortcomings that overcoming the prior art and deficiency, provide one plant for purifying molasses-spirit
The bacterial strain of waste water.
Another object of the present invention is to provide described for purifying the application of the bacterial strain of molasses alcohol waste water.
The purpose of the invention is achieved by the following technical solution: one plant for purifying the bacterial strain of molasses alcohol waste water, title
For lysine bacillus (Lysinibacillus sp.) S6, deposit number was CCTCC NO:M2017085, in 2017 3
The moon is preserved in the China typical culture collection center positioned at Wuhan, China Wuhan University on the 6th.
Described application of the bacterial strain in molasses alcohol water process for purifying molasses alcohol waste water, preferably comprise as
Lower specific steps:
(1) strain inoculated for being used to purify molasses alcohol waste water is fermented in fermentation medium；
(2) bacterium solution obtained after step (1) fermentation is inoculated into molasses alcohol waste water, fermentation process.
The composition of the fermentation medium in step (1) is preferably as follows: molasses alcohol waste water (COD=
Dosage 105851.15mg/L) is calculated so that fermentation medium COD is 105851.15mg/L, the MgSO of 1mol/L4
The CaCl of 2.00mL, 1mol/L20.10mL, 5 × M9 salting liquid 200mL add distilled water to be settled to 1000mL, pH 7.0.
The dosage of molasses alcohol waste water (COD=105851.15mg/L) is with fermented and cultured in the fermentation medium
Base COD is 105851.15mg/L to calculate.
The composition of 5 × M9 salting liquid is as follows: Na2HPO4·7H2O 12.80g, KH2PO43.00g NaCl
0.50g, NH4Cl 1.00g, distilled water are settled to 200mL.
The condition of the fermentation is preferably 37 DEG C, revolving speed is to cultivate in 200rpm aerobic environment shaking table.
The present invention has the following advantages and effects with respect to the prior art:
Bacterial strain provided by the invention can be in the microbial strains grown using molasses alcohol waste water as sole carbon source in sugar
Mulse essence waste water COD concentration is 105851.15mg/L, and temperature is 37 DEG C or so, in the case where not adding any microelement,
COD removal effect is ideal, and 48h is up to 64.22%.In addition, the bacterium also has certain denitrification dephosphorization function.
Detailed description of the invention
Fig. 1 is the form photo figure provided by the present invention for purifying the bacterial strain of molasses alcohol waste water；Wherein, figure (A) is
Gram-stained displaing micro photo figure (100 ×/1.25oil), figure (B) are bacterium colony photo figure.
Fig. 2 is that the more sequences of the present invention compare the bacterial strain for purifying molasses alcohol waste water of software MEGA5.0 building and are
System development tree, wherein branch's numerical value represents the confidence level after calculating 1000 times, and scale bar indicates that genetic distance, scale bar indicate
Genetic distance.
Fig. 3 is the growth curve chart provided by the present invention for purifying the bacterial strain of molasses alcohol waste water.
Fig. 4 is the detection provided by the present invention for purifying the bacterial strain removal molasses alcohol waste water COD of molasses alcohol waste water
Result figure.
Fig. 5 is the detection provided by the present invention for purifying the bacterial strain removal molasses alcohol waste water ammonia nitrogen of molasses alcohol waste water
Result figure.
Fig. 6 is the detection provided by the present invention for purifying the bacterial strain removal molasses alcohol waste water total phosphorus of molasses alcohol waste water
Result figure.
Specific embodiment
Below with reference to embodiment and Figure of description, the present invention is described in further detail, but embodiment party of the invention
Formula is without being limited thereto.
Culture medium used in the present invention is as follows:
Tame culture medium are as follows: it is dense that molasses alcohol waste water and the pig manure water of filtering according to the ratio between COD 3:2 are configured to total COD
Degree is the mixed-culture medium of 4000mg/L.By mixed-culture medium and rich medium respectively with volume ratio 1:4 (domestication culture medium
A), 2:4 (domestication culture medium B), 3:4 (domestication culture medium C), 4:0 (domestication culture medium D) proportion mix, and pH is adjusted to 7.00,
115 DEG C of low pressure moist heat sterilization 15min successively obtain domestication culture medium A, B, C, D respectively.
Rich medium are as follows: peptone 10.00g, beef extract 3.00g, yeast powder 5.00g, glucose 3.00g, sodium chloride
3.00g, dipotassium hydrogen phosphate 2.50g, soybean broth 3.00g, water are settled to 1000mL, pH7.00.
LB solid medium are as follows: tryptone 10.00g, yeast powder 5.00g, sodium chloride 5.00g, agar 11g, water constant volume
To 1000mL, pH 7.00.
Fermentation broth are as follows: the dosage of molasses alcohol waste water (COD=105851.15mg/L) is with fermentation medium
COD is 105851.15mg/L to calculate, the MgSO of 1mol/L4The CaCl of 2.00mL, 1mol/L20.10mL, 5 × M9 salting liquid
200mL adds distilled water to be settled to 1000mL, pH 7.0.
The composition of 5 × M9 salting liquid is as follows: Na2HPO4·7H2O 12.80g, KH2PO43.00g, NaCl 0.50g,
NH4Cl 1.00g, distilled water are settled to 200mL.
Fermentation solid culture medium are as follows: agar is added in fermentation broth, the concentration of agar is 1.1% (w/v).
Embodiment 1
(1) domestication culture: sludge is extracted (not from operation to IC reactor (dischargeable capacity is about the 20L) bottom of stationary phase
Acclimation sludge is derived from the running IC reactor bottom of Guangxi Gui Tang limited liability company, is rich in microbial flora), with sterile glass
Glass stick mixes after smashing activated sludge to pieces, stands 5min, takes its supernatant.Rich medium is accessed with the inoculum concentration of 5% (w/v)
In, it is placed under aerobic (at 37 DEG C, 200rpm shaking table shaken cultivation) environment and cultivates 2d.Domestication is accessed with the inoculum concentration of 5% (v/v)
In culture medium A, after cultivating 2d under the same terms.It shakes up and is transferred in domestication culture medium B with the inoculum concentration of 5% (v/v), identical item
After cultivating 3d under part.It shakes up and is transferred in domestication culture medium C with the inoculum concentration of 5% (v/v), cultivate 3d under the same terms.Finally
It shakes up to transfer with the inoculum concentration of 5% (v/v) and cultivate in only molasses alcohol waste water and the domestication culture medium of pig manure water mixed liquid
(i.e. domestication culture medium D) 3d, domestication stage terminate.
(2) isolation and purification culture: sample is derived from the zymocyte liquid for taming the whole stage, using dilution spread flat band method to sample
LB plate is applied after carrying out gradient dilution；10 are chosen respectively-3、10-4、10-5、10-6Four gradient samples, each gradient are arranged three and put down
Row, with 1% inoculum concentration coated plate, is arranged a blank control, is placed in 37 DEG C of shake cultures and observes daily growing state；When flat
When plate bacterium colony abundance is more, colonial morphology is observed, as far as possible chooses the different bacterial strain of form on plate with plate streak
Turn to draw on fresh rich medium after out, and continuously choose single colonie and carry out scribing line purifying, is placed under the conditions of aerobic environment
37 DEG C, 150~200rpm culture, at least repeat the above scribing operation 3 times or more.
(3) Morphological Identification: the bacterial strain of isolation and purification culture identifies gram-negative/positive bacteria with Gram's stain.By bacterium
Strain is observed bacterium colony size, shape, color and surface characteristics after cultivating 1-2d at 37 DEG C on LB solid medium and is described.
Gram's staining and colonial morphology are as shown in Figure 1.This plant of Gram's staining is red, shape be it is rod-shaped, be determined as negative bar
Shape bacterium.In LB cultured on solid medium, single colonie is round, and flat, edge is uneven, moistens, and surface is smooth, easy picking,
Tow sides are in light brown.
(4) molecular biology identification: using genomic DNA as template, universal primer 27f (5 '-is used
AGAGTTTGATCCTGGCTCAG-3 ') and 1492r (5 '-TACGGTTACCTTGTTACGACTT-3 ') PCR amplification 16S rDNA.
Product send to Sangon Biotech (Shanghai) Co., Ltd. and is sequenced.It is as follows that obtained sequence is sequenced, sequencing is obtained
16S rDNA segment be compared with known bacterium in Genbank database to obtain sample belong to categorization levels on letter
Breath.And it is as shown in Figure 2 with more sequences comparison software MEGA5.0 phylogenetic tree construction.
GCTGGCTCCAAAGGTTACCTCACCGACTTCGGGTGTTACAAACTCTCGTGGTGTGACGGGCGGTGTGT
ACAAGGCCCGGGAACGTATTCACCGCGGCATGCTGATCCGCGATTACTAGCGATTCCGGCTTCATGTAGGCGAGTT
GCAGCCTACAATCCGAACTGAGAACGACTTTATCGGATTAGCTCCCTCTCGCGAGTTGGCAACCGTTTGTATCGTC
CATTGTAGCACGTGTGTAGCCCAGGTCATAAGGGGCATGATGATTTGACGTCATCCCCACCTTCCTCCGGTTTGTC
ACCGGCAGTCACCTTAGAGTGCCCAACTAAATGATGGCAACTAAGATCAAGGGTTGCGCTCGTTGCGGGACTTAAC
CCAACATCTCACGACACGAGCTGACGACAACCATGCACCACCTGTCACCGTTGCCCCCGAAGGGGAAACTATATCT
CTACAGTGGTCAACGGGATGTCAAGACCTGGTAAGGTTCTTCGCGTTGCTTCGAATTAAACCACATGCTCCACCGC
TTGTGCGGGCCCCCGTCAATTCCTTTGAGTTTCAGTCTTGCGACCGTACTCCCCAGGCGGAGTGCTTAATGCGTTA
GCTGCAGCACTAAGGGGCGGAAACCCCCTAACACTTAGCACTCATCGTTTACGGCGTGGACTACCAGGGTATCTAA
TCCTGTTTGCTCCCCACGCTTTCGCGCCTCAGCGTCAGTTACAGACCAGAAAGTCGCCTTCGCCACTGGTGTTCCT
CCAAATCTCTACGCATTTCACCGCTACACTTGGAATTCCACTTTCCTCTTCTGCACTCAAGTCCCCCAGTTTCCAA
TGACCCTCCACGGTTGAGCCGTGGGCTTTCACATCAGACTTAAAGGACCGCCTGCGCGCGCTTTACGCCCAATAAT
TCCGGACAACGCTTGCCACCTACGTATTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTAATAAGGTACC
GTCAAGGTACAGCCAGTTACTACTGTACTTGTTCTTCCCTTACAACAGAGTTTTACGATCCGAAAACCTTCTTCAC
TCACGCGGCGTTGCTCCATCAGGCTTTCGCCCATTGTGGAAGATTCCCTACTGCTGCCTCCCGTAGGAGTCTGGGC
CGTGTCTCAGTCCCAGTGTGGCCGATCACCCTCTCAGGTCGGCTACGCATCGTCGCCTTGGTGAGCCGTTACCTCA
CCAACTAGCTAATGCGCCGCGGGCCCATCCTATAGCGACAGCCGAAACCGTCTTTCAGTCTTTCACCATGAAGCAA
AAGAGATTATTCGGTATTAGCCCCGGTTTCCCGGAGTTATCCCAAACTATAGGGTAGGTTGCCCACGTGT。
It is identified, determine that isolated Pseudomonas in lysine bacillus, is named as lysine bacillus
(Lysinibacillus sp.) S6, deposit number are CCTCC NO:M2017085, are preserved in being located on March 6th, 2017
The China typical culture collection center of state Hubei Wuhan Wuhan University.
Embodiment 2
It takes lysine bacillus (Lysinibacillus sp.) S6 bacterial strain to carry out growth curve measurement: will be put down positioned at LB
Microbionation on plate is that culture is cultivated in 200rpm/min aerobic environment shaking table in 37 DEG C, revolving speed in rich medium, can
Observe that the growth and breeding of bacterium has certain regularity, with OD600Value makees ordinate, makees abscissa with incubation time, is depicted as
Growth curve.
Growth curve chart in rich medium is as shown in Figure 3, it is seen then that the microorganism after domestication does not have adjustment period
Either adjustment period is very short, enters logarithmic phase after inoculation soon, and microorganism enters stationary phase after 48h.
Embodiment 3
It takes lysine bacillus (Lysinibacillus sp.) S6 bacterial strain to tame culture medium, is cultivated in 200rpm
3d.Aerobic bacteria bacterium solution is inoculated in fermentation broth with 1% inoculum concentration again, respectively at aerobic 37 DEG C of 200rpm shaking tables
Shaken cultivation；Its COD, ammonia nitrogen, total phosphorus are measured daily, measure 6d altogether.Observe the variation of its COD, ammonia nitrogen, total phosphorus.
COD removal effect in the fermentation medium is as shown in Figure 4: the COD removal rate of 1d is 45.06%, 2d's
COD removal rate is 64.22%,.With the extension of processing time, COD removal rate amplification is on a declining curve, after fermentation process 6 days,
COD removal rate is only up to 71.00%.In 3d~6d, with the extension of fermentation time, nutriment therein is being reduced,
The activity of a part of bacterium may just decline.
Ammonia nitrogen removal effect in the fermentation medium is as shown in Figure 5: with the extension of fermentation time in 1d~4d,
The removal rate of ammonia nitrogen is being gradually increased.Reaching maximum value in 4d is 21.90%.The removal rate of 5d~6d ammonia nitrogen compares
4d is declined.
Total phosphorus removal effect in the fermentation medium is as shown in Figure 6: the activity of microorganism in 1d~2d of fermentation
Height, to the good absorption effect of total phosphorus, removal rate is higher.It is being followed by subsequent processing in the time, the removal rate decline of total phosphorus.All in all,
The bacterial strain is lower than 5% to the removal rate of total phosphorus, and removal effect is undesirable.
The above embodiment is a preferred embodiment of the present invention, but embodiments of the present invention are not by above-described embodiment
Limitation, other any changes, modifications, substitutions, combinations, simplifications made without departing from the spirit and principles of the present invention,
It should be equivalent substitute mode, be included within the scope of the present invention.
<110>Guangxi University
<120>one plants for purifying bacterial strain and its application of molasses alcohol waste water
<140> 2017101524012
<141> 2017-03-15
<160> 3
<170> SIPOSequenceListing 1.0
<210> 1
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<212> DNA
<213>artificial sequence (Artificial Sequence)
<220>
<223>primer 2 7f
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agagtttgat cctggctcag 20
<210> 2
<211> 22
<212> DNA
<213>artificial sequence (Artificial Sequence)
<220>
<223>primer 1492r
<400> 2
tacggttacc ttgttacgac tt 22
<210> 3
<211> 1354
<212> DNA
<213>lysine bacillus (Lysinibacillus sp.)
<400> 3
gctggctcca aaggttacct caccgacttc gggtgttaca aactctcgtg gtgtgacggg 60
cggtgtgtac aaggcccggg aacgtattca ccgcggcatg ctgatccgcg attactagcg 120
attccggctt catgtaggcg agttgcagcc tacaatccga actgagaacg actttatcgg 180
attagctccc tctcgcgagt tggcaaccgt ttgtatcgtc cattgtagca cgtgtgtagc 240
ccaggtcata aggggcatga tgatttgacg tcatccccac cttcctccgg tttgtcaccg 300
gcagtcacct tagagtgccc aactaaatga tggcaactaa gatcaagggt tgcgctcgtt 360
gcgggactta acccaacatc tcacgacacg agctgacgac aaccatgcac cacctgtcac 420
cgttgccccc gaaggggaaa ctatatctct acagtggtca acgggatgtc aagacctggt 480
aaggttcttc gcgttgcttc gaattaaacc acatgctcca ccgcttgtgc gggcccccgt 540
caattccttt gagtttcagt cttgcgaccg tactccccag gcggagtgct taatgcgtta 600
gctgcagcac taaggggcgg aaacccccta acacttagca ctcatcgttt acggcgtgga 660
ctaccagggt atctaatcct gtttgctccc cacgctttcg cgcctcagcg tcagttacag 720
accagaaagt cgccttcgcc actggtgttc ctccaaatct ctacgcattt caccgctaca 780
cttggaattc cactttcctc ttctgcactc aagtccccca gtttccaatg accctccacg 840
gttgagccgt gggctttcac atcagactta aaggaccgcc tgcgcgcgct ttacgcccaa 900
taattccgga caacgcttgc cacctacgta ttaccgcggc tgctggcacg tagttagccg 960
tggctttcta ataaggtacc gtcaaggtac agccagttac tactgtactt gttcttccct 1020
tacaacagag ttttacgatc cgaaaacctt cttcactcac gcggcgttgc tccatcaggc 1080
tttcgcccat tgtggaagat tccctactgc tgcctcccgt aggagtctgg gccgtgtctc 1140
agtcccagtg tggccgatca ccctctcagg tcggctacgc atcgtcgcct tggtgagccg 1200
ttacctcacc aactagctaa tgcgccgcgg gcccatccta tagcgacagc cgaaaccgtc 1260
tttcagtctt tcaccatgaa gcaaaagaga ttattcggta ttagccccgg tttcccggag 1320
ttatcccaaa ctatagggta ggttgcccac gtgt 1354
Claims (5)
1. one plant for purifying the bacterial strain of molasses alcohol waste water, it is characterised in that: described is used to purify molasses alcohol waste water
Entitled lysine bacillus (Lysinibacillus sp.) S6 of bacterial strain, deposit number are CCTCC NO:M2017085,
The China typical culture collection center positioned at Wuhan, China Wuhan University is preserved on March 6th, 2017.
2. the application of bacterial strain in molasses alcohol water process described in claim 1 for purifying molasses alcohol waste water.
3. according to claim 2 for purifying bacterial strain the answering in molasses alcohol water process of molasses alcohol waste water
With, it is characterised in that include following specific steps:
(1) strain inoculated for being used to purify molasses alcohol waste water is fermented in fermentation medium；
(2) bacterium solution obtained after step (1) fermentation is inoculated into molasses alcohol waste water, fermentation process.
4. according to claim 3 for purifying bacterial strain the answering in molasses alcohol water process of molasses alcohol waste water
With, it is characterised in that:
The composition of the fermentation medium in step (1) is as follows: the dosage of molasses alcohol waste water is with fermentation medium COD
It is calculated for 105851.15mg/L, the MgSO of 1mol/L4The CaCl of 2.00mL, 1mol/L20.10mL, 5 × M9 salting liquid
200mL adds distilled water to be settled to 1000mL, pH 7.0.
5. according to claim 3 for purifying bacterial strain the answering in molasses alcohol water process of molasses alcohol waste water
With, it is characterised in that:
The condition of the fermentation is 37 DEG C, revolving speed is to cultivate in 200rpm aerobic environment shaking table.
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| CN103030211A (en) * | 2012-12-10 | 2013-04-10 | 广西大学 | Method for treating molasses alcohol wastewater |
| CN104630110A (en) * | 2015-02-06 | 2015-05-20 | 重庆大学 | COD (chemical oxygen demand) degradation microbial inoculant, and preparation method and application thereof |
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| CN103937697B (en) * | 2014-01-14 | 2017-01-25 | 暨南大学 | Bacterial strain for degrading dye with high efficiency |
| CN104593296B (en) * | 2014-12-31 | 2017-08-22 | 盐城工学院 | Paper waste dirt fills reed Tanaka's typical bacteria --- screening of spindle lysine bacillus and application thereof |
| CN108102942A (en) * | 2017-07-21 | 2018-06-01 | 广西大学 | One plant of bacterial strain and its application for being used to purify molasses alcohol waste water |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103030211A (en) * | 2012-12-10 | 2013-04-10 | 广西大学 | Method for treating molasses alcohol wastewater |
| CN104630110A (en) * | 2015-02-06 | 2015-05-20 | 重庆大学 | COD (chemical oxygen demand) degradation microbial inoculant, and preparation method and application thereof |
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