CN108118013A - One plant of bacterial strain and its application for being used to purify molasses alcohol waste water - Google Patents
One plant of bacterial strain and its application for being used to purify molasses alcohol waste water Download PDFInfo
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- CN108118013A CN108118013A CN201810068347.8A CN201810068347A CN108118013A CN 108118013 A CN108118013 A CN 108118013A CN 201810068347 A CN201810068347 A CN 201810068347A CN 108118013 A CN108118013 A CN 108118013A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F2103/00—Nature of the water, waste water, sewage or sludge to be treated
- C02F2103/34—Nature of the water, waste water, sewage or sludge to be treated from industrial activities not provided for in groups C02F2103/12 - C02F2103/32
- C02F2103/36—Nature of the water, waste water, sewage or sludge to be treated from industrial activities not provided for in groups C02F2103/12 - C02F2103/32 from the manufacture of organic compounds
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Abstract
The present invention relates to one plant of microbial strains and its applications, and in particular to a kind of lysine bacillus (Lysinibacillus sp.) S6 and its application in molasses alcohol waste water belong to technical field of microbe application.Being the object of the present invention is to provide a kind of bacterial strain can be in microbial strains lysine bacillus (Lysinibacillus sp.) S6 grown using molasses alcohol waste water as sole carbon source, and its corresponding condition of culture and in the case of being disclosed in and not adding any trace element, COD removal effects are ideal, and 48h is up to 64.22%.In addition, the bacterium also has certain denitrification dephosphorization function.
Description
Technical field
The invention belongs to microorganism field, more particularly to one plant bacterial strain and its application for being used to purify molasses alcohol waste water.
Background technology
Sugaring by-product-molasses, are usually used to fermenting and producing alcohol, a large amount of height can be released during alcohol is produced
The organic wastewater of concentration is referred to as molasses alcohol waste water.Substantial amounts of waste water can cause water eutrophication without processing directly discharge
Change, destroy water body and terrestrial ecosystems.Molasses alcohol waste water is directly used in agriculture filling, can cause soil acidification hardened phenomenon.Such as
Fruit is used for pouring sugarcane field, can increase sugarcane yield, but can reduce the sugar content in sugarcane.Currently processed molasses alcohol
The method rationalization method and bioremediation of water.Physico-chemical method is compared to, bioremediation has more safety height
Effect, the cycle is short, at low cost, will not cause secondary pollution, and biogas energy can also be generated in processing procedure, treated thalline
It is good forage protein again.These advantages cause bioremediation to become the key points and difficulties of current research.For biology
For processing method, good microorganism fungus kind is to ensure that biological wastewater treatment is successfully crucial, is screened from environment excellent
Microbial resources are a kind of important channels.So recent researches person, which will filter out, has molasses alcohol waste water efficiently life
Starting point of the microorganism (bacterium, fungi etc.) of object degradation capability as research.Wang Weijia is from molasses alcohol oxidation pond
Isolated two plants of bacteriums PSB-C and PSB-D, this two plants of bacteriums belong to the Rhodopseudomonas of photosynthetic bacteria.In optimum response
Under the conditions of (COD concentration is 20800mg/L, and reaction temperature is 30 DEG C, reaction time 3d) two plants of bacterium to the COD removal rates point of waste water
It is not 55% and 54%.Liu Jianfu filters out 8 plants of bacteriums from the reactor granules sludge of UASB, is respectively skin Bacillus, stick
Bacillus, Exiguobacterium sp category, lactobacillus, Cellulomonas, Propionibacterium, red long life Pseudomonas are in waste water COD concentration
24000mg/L is handled under the conditions of 37 DEG C, and the removal rate of COD is 25.1% (query：See document composite bacteria agent COD removal rates 90%
More than, it is therefore desirable to documentation ＆ info is provided, information is to deserved more clearer and more definite).The flora that Fan Yanxia is enriched with from IC reactors sludge,
The distribution of bacterium belong to Erysipelotrichales, Clostridiales, Lactobacillales, Xanthomonadales,
In 7 mesh of Burkholderiales, Enterobacteriales, Bacteroidales, under optimum reaction conditions, simultaneously
Nutriment is added, handles 7d, the removal rate of COD is 38.5%.Mohana, which screens to obtain flora DMC, to be inoculated into while adds Portugal
In grape sugar and inorganic salts molasses alcohol waste water, 37 DEG C of processing 4d, COD removal rates are 51%, decolorization rate of wastewater 67%；The flora
It is compared through 16SrDNA, including pseudomonas aeruginosa (Pseudomonas aeruginosa PAO1), thermophilic malt Flavobacterium
(stenotrophomonas maltophila), proteus mirabilis (Proteus mirabilis).Chopra white rots are true
Bacterium and manyzoned polypore bacteria (C.versicolor) processing molasses alcohol waste water add glucose and peptone as nutriment, after 8d
COD and the removal rate of colourity are all 53%.Ghosh is with pseudomonas putida (Pseudomonas putida) and Aeromonas
(Aeromonas sp.) two-step pretreatment molasses alcohol waste water adds 1% glucose in waste water.Finally, disliked after handling for 24 hours
The COD removal rates of smelly pseudomonad are 44.4%, chroma removal rate 60%, and the COD removal rates of Aeromonas are 44.4%.
At present, there are no the reports that application lysine bacillus reduces molasses alcohol waste water COD.
The content of the invention
The shortcomings that primary and foremost purpose of the present invention is to overcome the prior art and deficiency, provide one plant for purifying molasses-spirit
The bacterial strain of waste water.
Another object of the present invention is to provide the application of the bacterial strain for being used to purify molasses alcohol waste water.
The purpose of the present invention is achieved through the following technical solutions：One plant of bacterial strain for being used to purify molasses alcohol waste water, title
For lysine bacillus (Lysinibacillus sp.) S6, deposit number is CCTCC NO：M2017085, in 2017 3
The moon is preserved in the China typical culture collection center positioned at Wuhan, China Wuhan University on the 6th.
It is described for purifying application of the bacterial strain of molasses alcohol waste water in molasses alcohol water process, preferably comprise as
Lower specific steps：
(1) ferment described for purifying the inoculation of molasses alcohol waste water in fermentation medium；
(2) bacterium solution obtained after step (1) is fermented is inoculated into molasses alcohol waste water, fermentation process.
The composition of the fermentation medium in step (1) is preferably as follows：Molasses alcohol waste water (COD=
Dosage 105851.15mg/L) is calculated using fermentation medium COD as 105851.15mg/L, the MgSO of 1mol/L4
The CaCl of 2.00mL, 1mol/L20.10mL, 5 × M9 salting liquid 200mL add distilled water to be settled to 1000mL, pH 7.0.
The dosage of molasses alcohol waste water (COD=105851.15mg/L) is with fermented and cultured in the fermentation medium
Base COD calculates for 105851.15mg/L.
The composition of 5 × M9 salting liquids is as follows：Na2HPO4·7H2O 12.80g, KH2PO43.00g NaCl
0.50g, NH4Cl 1.00g, distilled water are settled to 200mL.
The condition of the fermentation is preferably 37 DEG C, rotating speed is cultivates in 200rpm aerobic environment shaking tables.
The present invention is had the following advantages compared with the prior art and effect：
Bacterial strain provided by the invention can be in the microbial strains grown using molasses alcohol waste water as sole carbon source in rum
Smart waste water COD concentration is 105851.15mg/L, and temperature is 37 DEG C or so, and in the case of not adding any trace element, COD is gone
Except effect is ideal, 48h is up to 64.22%.In addition, the bacterium also has certain denitrification dephosphorization function.
Description of the drawings
Fig. 1 is the form photo figure provided by the present invention for purifying the bacterial strain of molasses alcohol waste water；Wherein, figure (A) is
Gram-stained displaing micro photo figure (100 ×/1.25oil), figure (B) are bacterium colony photo figure.
Fig. 2 is that the present invention is for purify the bacterial strain of molasses alcohol waste water with what more sequences compared software MEGA5.0 structures
System development tree, wherein, branch's numerical value represents the confidence level after calculating 1000 times, and engineer's scale represents genetic distance, and engineer's scale represents
Genetic distance.
Fig. 3 is the growth curve chart provided by the present invention for purifying the bacterial strain of molasses alcohol waste water.
Fig. 4 is the detection provided by the present invention for purifying the bacterial strain removal molasses alcohol waste water COD of molasses alcohol waste water
Result figure.
Fig. 5 is the detection provided by the present invention for purifying the bacterial strain removal molasses alcohol waste water ammonia nitrogen of molasses alcohol waste water
Result figure.
Fig. 6 is the detection provided by the present invention for purifying the bacterial strain removal molasses alcohol waste water total phosphorus of molasses alcohol waste water
Result figure.
Specific embodiment
With reference to embodiment and Figure of description, the present invention is described in further detail, but the embodiment party of the present invention
Formula is without being limited thereto.
Culture medium used in the present invention is as follows：
Taming culture medium is：By molasses alcohol waste water and the pig manure water of filtering according to the ratio between COD 3：2 to be configured to total COD dense
Spend the mixed-culture medium for 4000mg/L.By mixed-culture medium and rich medium respectively with volume ratio 1：4 (domestication culture mediums
A)、2：4 (domestication culture medium B), 3：4 (domestication culture medium Cs), 4:0 (domestication culture medium D) proportioning mixing, 7.00 are adjusted to by pH,
115 DEG C of low pressure moist heat sterilization 15min obtain domestication culture medium A, B, C, D successively respectively.
Rich medium is：Peptone 10.00g, beef extract 3.00g, dusty yeast 5.00g, glucose 3.00g, sodium chloride
3.00g, dipotassium hydrogen phosphate 2.50g, soybean broth 3.00g, water are settled to 1000mL, pH7.00.
LB solid mediums are：Tryptone 10.00g, dusty yeast 5.00g, sodium chloride 5.00g, agar 11g, water constant volume
To 1000mL, pH 7.00.
Fermentation broth is：The dosage of molasses alcohol waste water (COD=105851.15mg/L) is with fermentation medium
COD calculates for 105851.15mg/L, the MgSO of 1mol/L4The CaCl of 2.00mL, 1mol/L20.10mL, 5 × M9 salting liquid
200mL adds distilled water to be settled to 1000mL, pH 7.0.
The composition of 5 × M9 salting liquids is as follows：Na2HPO4·7H2O 12.80g, KH2PO43.00g, NaCl 0.50g,
NH4Cl 1.00g, distilled water are settled to 200mL.
Fermentation solid culture medium is：Agar is added in fermentation broth, the concentration of agar is 1.1% (w/v).
Embodiment 1
(1) domestication culture：From operation to IC reactors (dischargeable capacity is about 20L) bottom of stationary phase extraction sludge (not
Acclimation sludge is derived from the running IC reactor bottoms of Guangxi Gui Tang limited companies, rich in microorganism species), with sterile glass
Glass stick smashs activated sludge to pieces rear mixing, stands 5min, takes its supernatant.Rich medium is accessed with the inoculum concentration of 5% (w/v)
In, it is placed under aerobic (at 37 DEG C, 200rpm shaking tables shaken cultivation) environment and cultivates 2d.Domestication is accessed with the inoculum concentration of 5% (v/v)
In culture medium A, after cultivating 2d under the same terms.It shakes up and is transferred with the inoculum concentration of 5% (v/v) in domestication culture medium B, identical item
After 3d being cultivated under part.It shakes up and is transferred with the inoculum concentration of 5% (v/v) in domestication culture medium C, 3d is cultivated under the same terms.Finally
It shakes up and only molasses alcohol waste water is transferred with being cultivated in the domestication culture medium of pig manure water mixed liquid with the inoculum concentration of 5% (v/v)
(taming culture medium D) 3d, domestication stage terminate.
(2) isolation and purification culture：Sample is derived from the zymocyte liquid in domestication whole stage, using dilution spread flat band method to sample
LB plates are applied after carrying out gradient dilution；10 are chosen respectively-3、10-4、10-5、10-6Four gradient samples, each gradient set three to put down
Row with 1% inoculum concentration coated plate, sets a blank control, is placed in 37 DEG C of shake cultures and observes daily growing state；When flat
When plate bacterium colony abundance is more, colonial morphology is observed, as far as possible chooses the different bacterial strain of form on tablet with plate streak
Turn to draw on fresh rich medium after going out, and continuously choose single bacterium colony and carry out line purifying, be placed under the conditions of aerobic environment
37 DEG C, 150~200rpm culture, at least repeat more than scribing operation 3 times and more than.
(3) Morphological Identification：The bacterial strain of isolation and purification culture differentiates gram-negative/positive bacteria with Gram's stain.By bacterium
Strain is observed bacterium colony size, shape, color and surface characteristics after cultivating 1-2d at 37 DEG C on LB solid mediums and is described.
Gram's staining and colonial morphology are as shown in Figure 1.This plant of Gram's staining is red, and shape is rod-shaped, is determined as negative bar
Shape bacterium.In LB cultured on solid medium, single bacterium colony is circular, and flat, edge is uneven, and moistening, surface is smooth, easy picking,
Tow sides are in light brown.
(4) molecular biology identification：Using genomic DNA as template, using universal primer 27f (5 '-
AGAGTTTGATCCTGGCTCAG-3 ') and 1492r (5 '-TACGGTTACCTTGTTACGACTT-3 ') PCR amplification 16S rDNA.
Product send to Sangon Biotech (Shanghai) Co., Ltd. and is sequenced.It is as follows that obtained sequence is sequenced, sequencing is obtained
16S rDNA segments be compared to obtain letter of the sample on categorization levels are belonged to known bacterium in Genbank databases
Breath.It is and as shown in Figure 2 with more sequences comparison software MEGA5.0 phylogenetic tree constructions.
GCTGGCTCCAAAGGTTACCTCACCGACTTCGGGTGTTACAAACTCTCGTGGTGTGACGGGCGGTGTGTACAAGGCCC
GGGAACGTATTCACCGCGGCATGCTGATCCGCGATTACTAGCGATTCCGGCTTCATGTAGGCGAGTTGCAGCCTACA
ATCCGAACTGAGAACGACTTTATCGGATTAGCTCCCTCTCGCGAGTTGGCAACCGTTTGTATCGTCCATTGTAGCAC
GTGTGTAGCCCAGGTCATAAGGGGCATGATGATTTGACGTCATCCCCACCTTCCTCCGGTTTGTCACCGGCAGTCAC
CTTAGAGTGCCCAACTAAATGATGGCAACTAAGATCAAGGGTTGCGCTCGTTGCGGGACTTAACCCAACATCTCACG
ACACGAGCTGACGACAACCATGCACCACCTGTCACCGTTGCCCCCGAAGGGGAAACTATATCTCTACAGTGGTCAAC
GGGATGTCAAGACCTGGTAAGGTTCTTCGCGTTGCTTCGAATTAAACCACATGCTCCACCGCTTGTGCGGGCCCCCG
TCAATTCCTTTGAGTTTCAGTCTTGCGACCGTACTCCCCAGGCGGAGTGCTTAATGCGTTAGCTGCAGCACTAAGGG
GCGGAAACCCCCTAACACTTAGCACTCATCGTTTACGGCGTGGACTACCAGGGTATCTAATCCTGTTTGCTCCCCAC
GCTTTCGCGCCTCAGCGTCAGTTACAGACCAGAAAGTCGCCTTCGCCACTGGTGTTCCTCCAAATCTCTACGCATTT
CACCGCTACACTTGGAATTCCACTTTCCTCTTCTGCACTCAAGTCCCCCAGTTTCCAATGACCCTCCACGGTTGAGC
CGTGGGCTTTCACATCAGACTTAAAGGACCGCCTGCGCGCGCTTTACGCCCAATAATTCCGGACAACGCTTGCCACC
TACGTATTACCGCGGCTGCTGGCACGTAGTTAGCCGTGGCTTTCTAATAAGGTACCGTCAAGGTACAGCCAGTTACT
ACTGTACTTGTTCTTCCCTTACAACAGAGTTTTACGATCCGAAAACCTTCTTCACTCACGCGGCGTTGCTCCATCAG
GCTTTCGCCCATTGTGGAAGATTCCCTACTGCTGCCTCCCGTAGGAGTCTGGGCCGTGTCTCAGTCCCAGTGTGGCC
GATCACCCTCTCAGGTCGGCTACGCATCGTCGCCTTGGTGAGCCGTTACCTCACCAACTAGCTAATGCGCCGCGGGC
CCATCCTATAGCGACAGCCGAAACCGTCTTTCAGTCTTTCACCATGAAGCAAAAGAGATTATTCGGTATTAGCCCCG
GTTTCCCGGAGTTATCCCAAACTATAGGGTAGGTTGCCCACGTGT。
It is identified, determine that isolated Pseudomonas in lysine bacillus, is named as lysine bacillus
(Lysinibacillus sp.) S6, deposit number are CCTCC NO:M2017085 is preserved on March 6th, 2017 in being located at
The China typical culture collection center of state Hubei Wuhan Wuhan University.
Embodiment 2
Lysine bacillus (Lysinibacillus sp.) S6 bacterial strains is taken to carry out growth curve measure：It will be put down positioned at LB
Microbionation on plate cultivates culture in rich medium in 37 DEG C, rotating speed is 200rpm/min aerobic environment shaking tables, can
Observing the growth and breeding of bacterium has certain regularity, with OD600Value makees ordinate, makees abscissa with incubation time, is depicted as
Growth curve.
Growth curve chart in rich medium is as shown in Figure 3, it is seen then that the microorganism after domestication does not have adjustment period
Either adjustment period is very short, enters logarithmic phase after inoculation soon, and microorganism enters stationary phase after 48h.
Embodiment 3
Lysine bacillus (Lysinibacillus sp.) S6 bacterial strains is taken to tame culture medium, are cultivated in 200rpm
3d.Aerobic bacteria bacterium solution is inoculated in fermentation broth with 1% inoculum concentration again, respectively at aerobic 37 DEG C of 200rpm shaking tables
Shaken cultivation；Its COD, ammonia nitrogen, total phosphorus are measured daily, measure 6d altogether.Observe the variation of its COD, ammonia nitrogen, total phosphorus.
COD removal effects in the fermentation medium are as shown in Figure 4：The COD removal rates of 1d are 45.06%, 2d's
COD removal rates are 64.22%,.With the extension of processing time, COD removal rate amplification is on a declining curve, and fermentation process is after 6 days,
COD removal rates are only up to 71.00%.In 3d~6d, with the extension of fermentation time, nutriment therein is being reduced,
The activity of a part of bacterium may just decline.
Ammonia nitrogen removal effect in the fermentation medium is as shown in Figure 5：With the extension of fermentation time in 1d~4d,
The removal rate of ammonia nitrogen is being gradually increased.Reach maximum in 4d for 21.90%.The removal rate of 5d~6d ammonia nitrogens compares
4d is declined.
Total phosphorus removal effect in the fermentation medium is as shown in Figure 6：The activity of microorganism in 1d~2d of fermentation
Height, to the good absorption effect of total phosphorus, removal rate is higher.It is being followed by subsequent processing in the time, the removal rate of total phosphorus declines.All in all,
For the bacterial strain to the removal rate of total phosphorus less than 5%, removal effect is undesirable.
Above-described embodiment is the preferable embodiment of the present invention, but embodiments of the present invention and from above-described embodiment
Limitation, other any Spirit Essences without departing from the present invention with made under principle change, modification, replacement, combine, simplification,
Equivalent substitute mode is should be, is included within protection scope of the present invention.
<110>Guangxi University
<120>One plant of bacterial strain and its application for being used to purify molasses alcohol waste water
<140> 2017101524012
<141> 2017-03-15
<160> 3
<170> SIPOSequenceListing 1.0
<210> 1
<211> 20
<212> DNA
<213>Artificial sequence (Artificial Sequence)
<220>
<223>Primer 2 7f
<400> 1
agagtttgat cctggctcag 20
<210> 2
<211> 22
<212> DNA
<213>Artificial sequence (Artificial Sequence)
<220>
<223>Primer 1492r
<400> 2
tacggttacc ttgttacgac tt 22
<210> 3
<211> 1354
<212> DNA
<213>Lysine bacillus (Lysinibacillus sp.)
<400> 3
gctggctcca aaggttacct caccgacttc gggtgttaca aactctcgtg gtgtgacggg 60
cggtgtgtac aaggcccggg aacgtattca ccgcggcatg ctgatccgcg attactagcg 120
attccggctt catgtaggcg agttgcagcc tacaatccga actgagaacg actttatcgg 180
attagctccc tctcgcgagt tggcaaccgt ttgtatcgtc cattgtagca cgtgtgtagc 240
ccaggtcata aggggcatga tgatttgacg tcatccccac cttcctccgg tttgtcaccg 300
gcagtcacct tagagtgccc aactaaatga tggcaactaa gatcaagggt tgcgctcgtt 360
gcgggactta acccaacatc tcacgacacg agctgacgac aaccatgcac cacctgtcac 420
cgttgccccc gaaggggaaa ctatatctct acagtggtca acgggatgtc aagacctggt 480
aaggttcttc gcgttgcttc gaattaaacc acatgctcca ccgcttgtgc gggcccccgt 540
caattccttt gagtttcagt cttgcgaccg tactccccag gcggagtgct taatgcgtta 600
gctgcagcac taaggggcgg aaacccccta acacttagca ctcatcgttt acggcgtgga 660
ctaccagggt atctaatcct gtttgctccc cacgctttcg cgcctcagcg tcagttacag 720
accagaaagt cgccttcgcc actggtgttc ctccaaatct ctacgcattt caccgctaca 780
cttggaattc cactttcctc ttctgcactc aagtccccca gtttccaatg accctccacg 840
gttgagccgt gggctttcac atcagactta aaggaccgcc tgcgcgcgct ttacgcccaa 900
taattccgga caacgcttgc cacctacgta ttaccgcggc tgctggcacg tagttagccg 960
tggctttcta ataaggtacc gtcaaggtac agccagttac tactgtactt gttcttccct 1020
tacaacagag ttttacgatc cgaaaacctt cttcactcac gcggcgttgc tccatcaggc 1080
tttcgcccat tgtggaagat tccctactgc tgcctcccgt aggagtctgg gccgtgtctc 1140
agtcccagtg tggccgatca ccctctcagg tcggctacgc atcgtcgcct tggtgagccg 1200
ttacctcacc aactagctaa tgcgccgcgg gcccatccta tagcgacagc cgaaaccgtc 1260
tttcagtctt tcaccatgaa gcaaaagaga ttattcggta ttagccccgg tttcccggag 1320
ttatcccaaa ctatagggta ggttgcccac gtgt 1354
Claims (5)
1. one plant of bacterial strain for being used to purify molasses alcohol waste water, it is characterised in that：It is described for purifying molasses alcohol waste water
Entitled lysine bacillus (Lysinibacillus sp.) S6 of bacterial strain, deposit number are CCTCC NO：M2017085,
The China typical culture collection center positioned at Wuhan, China Wuhan University is preserved on March 6th, 2017.
2. application of the bacterial strain described in claim 1 for being used to purify molasses alcohol waste water in molasses alcohol water process.
3. the bacterial strain for being used to purify molasses alcohol waste water answering in molasses alcohol water process according to claim 2
With, it is characterised in that include following specific steps：
(1) ferment described for purifying the inoculation of molasses alcohol waste water in fermentation medium；
(2) bacterium solution obtained after step (1) is fermented is inoculated into molasses alcohol waste water, fermentation process.
4. the bacterial strain for being used to purify molasses alcohol waste water answering in molasses alcohol water process according to claim 3
With, it is characterised in that：
The composition of the fermentation medium in step (1) is as follows：The dosage of molasses alcohol waste water is with fermentation medium COD
It is calculated for 105851.15mg/L, the MgSO of 1mol/L4The CaCl of 2.00mL, 1mol/L20.10mL, 5 × M9 salting liquid
200mL adds distilled water to be settled to 1000mL, pH 7.0.
5. the bacterial strain for being used to purify molasses alcohol waste water answering in molasses alcohol water process according to claim 3
With, it is characterised in that：
The condition of the fermentation is 37 DEG C, rotating speed is to be cultivated in 200rpm aerobic environment shaking tables.
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| CN104630110A (en) * | 2015-02-06 | 2015-05-20 | 重庆大学 | COD (chemical oxygen demand) degradation microbial inoculant, and preparation method and application thereof |
| CN108102942A (en) * | 2017-07-21 | 2018-06-01 | 广西大学 | One plant of bacterial strain and its application for being used to purify molasses alcohol waste water |
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Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
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