CN108102951A - A kind of Medium of Bifidobacterium containing Fructus Fici extract - Google Patents
A kind of Medium of Bifidobacterium containing Fructus Fici extract Download PDFInfo
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Abstract
The invention discloses a kind of Medium of Bifidobacterium containing Fructus Fici extract, are made of the raw material of following mass concentration：40~50g/L of Fructus Fici extract, 20~30g/L of carbon source, 10~30mg/L of multi-vitamins, 50~60g/L of colostrum, 10~15g/L of compound amino acid, 2~5g/L of potassium dihydrogen phosphate, 0.1~1g/L of magnesium chloride, 0.1~0.3g/L of calcium chloride, 0.2~0.4g/L of zinc sulfate, 0.1~0.3g/L of frerrous chloride, pH value 6.2~6.5；The Fructus Fici extract is by supercritical CO2It is obtained by extraction.For the Bifidobacterium of the present invention using Fructus Fici extract as proliferation factor, Bifidobacterium cultivation effect is good.
Description
Technical field
The present invention relates to biological technical fields, and in particular to a kind of Medium of Bifidobacterium containing Fructus Fici extract.
Background technology
Bifidobacterium (Bifidobacterium) is the excrement by French scholar Tissier from breast milk nutrition youngster in 1899
In the Gram-positive bacillus of a kind of anaerobism isolated, end usually bifurcated, therefore named Bifidobacterium.Bifidobacterium is in human body intestines
Lactic acid and acetic acid can be generated after interior fermentation, the utilization rate of calcium, phosphorus, iron can be improved, there is treatment chronic diarrhea, treatment constipation, protect
Protect liver is dirty, prevention and cure of cardiovascular disease, the effect for improving lactose digestion etc..Bifidobacterium is distributed in the quantity of stomach and intestine with age level
Growth and reduce, it is breast milk nutrition youngster to be distributed most.It has been found that Bifidobacterium there are 32 hypotypes, contain Bifidobacterium
Up to 70 kinds of biological agent.Bifidobacterium infantis accounts for 60 the percent of total enterobacteriaceae, and 60 years old or more old man Bifidobacterium is only
Account for 7 percent points nine.
Ficus berry trees, edible rate are up to more than 92%, and fruit thin skin is seedless, and meat is soft, and flavor is sweet, tool
There are very high nutritive value and medical value, cultivation fig has very high economy, ecology and social benefit.First, no flower
Fruit has very high nutritive value, its fruit is rich in sugar, protein, amino acid, vitamin and mineral element.According to Shandong woods section
Measured, the soluble solid content of ripe fig is up to 24%, most of kind sugar contents between 15%~22%,
More than 1 times of many one, two generation fruit varieties.Containing 18 kinds of amino acid in fruit, wherein it is essential amino acid to have 8 kinds.
Secondly, fig has high medical value.
The content of the invention
The present invention overcomes Bifidobacterium proliferation rate it is low the technical issues of, a kind of bifid containing Fructus Fici extract is provided
Baccilus medium.
To solve the above problems, the present invention adopts the following technical scheme that：
A kind of Bifidobacterium culture containing Fructus Fici extract is made of the raw material of following mass concentration：Fig extracts
40~50g/L of object, 20~30g/L of carbon source, 10~30mg/L of multi-vitamins, 50~60g/L of colostrum, compound amino acid 10~
15g/L, 2~5g/L of potassium dihydrogen phosphate, 0.1~1g/L of magnesium chloride, 0.1~0.3g/L of calcium chloride, 0.2~0.4g/L of zinc sulfate,
0.1~0.3g/L of frerrous chloride, pH value 6.2~6.5；
Wherein, extract obtains the Fructus Fici extract by the following method：By fig drying to percent water
For 10%, then fig be crushed into 200 mesh sieves, the fig after crushing is placed in supercritical extraction reactor, with mass ratio
For 3:1 ethyl acetate and ethyl alcohol carries out supercritical CO for entrainer230~40min of extraction obtains Fructus Fici extract；It is described
Supercritical CO2The condition of extraction is：35 DEG C of extraction temperature, extracting pressure 40Mpa, CO2Flow velocity be 30L/min；The folder
Mass ratio with agent and fig is 10~15:100.
Wherein, in the Fructus Fici extract, not less than 80%, remaining makes a living the mass percentage of fig polysaccharide
Alkaloids and glucoside.
Wherein, the molecular weight of the fig polysaccharide is not higher than 5000.
Wherein, the carbon source is glucose, sucrose, the one or more of galactolipin.
Wherein, the compound amino acid is by lysine, threonine, leucine, isoleucine, valine, methionine, color
Propylhomoserin, phenylalanine, cystine, cysteine, glycine, histidine, glutamic acid, arginine composition.
Wherein, the multi-vitamins are by vitamin A, vitamin C, vitamin B1, vitamin B12, vitamin E group
Into.
Wherein, fig drying is dried using microwave drying mode；The microwave power is 300~500W.
Colostrum is the milk in 3 days after cow calving, and nutritional ingredient is compared with ordinary milk, containing compared with high protein
Matter, and fat and sugar content is then relatively low.Colostrum is also containing substantial amounts of immune and growth factor, such as immunoglobulin, newborn iron egg
In vain, lysozyme, insulin-like growth factor, epidermal growth factor etc..Colostrum provides abundant nitrogen source for Bifidobacterium, simultaneously
Also contribute to the growth of hyperbolic bacillus containing abundant immune and growth factor in colostrum.
Bifidobacterium nutritional requirement is higher, and culture medium need to contain abundant amino acid, vitamin, glucide and buffer salt
Class, and in addition to basic nutritional need, also need addition bifid proliferation factor, such as carbohydrate containing N-Acetyl-D-glucosamine, egg
White matter is through proliferation factor in enzyme, glycoprotein, glycopeptide and plant extracts etc..
In existing technology, generally mostly using oligosaccharide as proliferation factor, including isomalto-oligosaccharide glucose, fructose
Oligosaccharide, sucrose oligosaccharide, cow's milk oligosaccharide, soyabean oligosaccharides, galactose oligosaccharides etc..
The present invention has the advantages that compared with prior art：
(1) Fructus Fici extract contained in culture medium of the invention is Bifidobacterium proliferation factor, while fig carries
The low-molecular polysaccharide in object is taken to be alternatively arranged as carbon source to be utilized by bifidobacterium fermentation, culture medium is using colostrum as main nitrogen, together
Vitamin, amino acid, inorganic salts and the suitable pH value that Shi Hanyou enriches, culture medium of the invention are greatly improved bifid bar
The appreciation rate of bacterium, bifidobacterium growth are bred.
(2) Fructus Fici extract of the invention uses supercritical CO2It is extracted, wherein using using mass ratio as 3:1
Ethyl acetate and ethyl alcohol be entrainer, use ethyl acetate, ethyl alcohol compared to single as entrainer, ethyl acetate and ethyl alcohol
Compound entrainer polarity is more preferable, especially good to the dissolubility of low-molecular polysaccharide to the polarity polysaccharide of fig kind, improves
The content of proliferation factor in Fructus Fici extract.
(3) it is low-molecular polysaccharide without melon and fruit polysaccharide to contain in the Fructus Fici extract in the present invention, is had to Bifidobacterium
There is higher proliferation function.
Specific embodiment
With reference to embodiment and experiment, the invention will be further described.
Embodiment 1
A kind of Medium of Bifidobacterium containing Fructus Fici extract is made of the raw material of following mass concentration：Fig carries
Take object 50g/L, carbon source 20g/L, multi-vitamins 30mg/L, colostrum 50g/L, compound amino acid 15g/L, potassium dihydrogen phosphate
2g/L, magnesium chloride 1g/L, calcium chloride 0.1g/L, zinc sulfate 0.4g/L, frerrous chloride 0.1g/L, pH value 6.5；Wherein, fig
The mass percentage of polysaccharide is not less than 80%, remaining is alkaloid and glucoside；The molecular weight of the fig polysaccharide is not high
In 5000；The carbon source is sucrose and galactolipin, and the compound amino acid is by lysine, threonine, leucine, different bright ammonia
Acid, valine, methionine, tryptophan, phenylalanine, cystine, cysteine, glycine, histidine, glutamic acid, arginine
Composition；The multi-vitamins are made of vitamin A, vitamin C, vitamin B1, vitamin B12, vitamin E
Extract obtains the Fructus Fici extract by the following method：It is the microwave of 300W by fig to use microwave power
Drying to percent water is 10%, then fig crushed 200 mesh sieves, and the fig after crushing is placed in overcritical extraction
It takes in kettle, using mass ratio as 3:1 ethyl acetate and ethyl alcohol carries out supercritical CO for entrainer2Extraction 40min obtains no flower
Berry extract；The supercritical CO2The condition of extraction is：35 DEG C of extraction temperature, extracting pressure 40Mpa, CO2Flow velocity be
30L/min；The entrainer and the mass ratio of fig are 10:100.
Embodiment 2
A kind of Medium of Bifidobacterium containing Fructus Fici extract is made of the raw material of following mass concentration：Fig carries
Take object 40g/L, carbon source 30g/L, multi-vitamins 10mg/L, colostrum 60g/L, compound amino acid 10g/L, potassium dihydrogen phosphate
5g/L, magnesium chloride 0.1g/L, calcium chloride 0.3g/L, zinc sulfate 0.2g/L, frerrous chloride 0.3g/L, pH value 6.2；Wherein, no flower
The mass percentage of fruit polysaccharide is not less than 80%, remaining is alkaloid and glucoside；The molecular weight of the fig polysaccharide is not
Higher than 5000；The carbon source is glucose, and the compound amino acid is by lysine, threonine, leucine, isoleucine, figured silk fabrics
Propylhomoserin, methionine, tryptophan, phenylalanine, cystine, cysteine, glycine, histidine, glutamic acid, arginine composition；
The multi-vitamins are made of vitamin A, vitamin C, vitamin B1, vitamin B12, vitamin E
Extract obtains the Fructus Fici extract by the following method：It is the microwave of 500W by fig to use microwave power
Drying to percent water is 10%, then fig crushed 200 mesh sieves, and the fig after crushing is placed in overcritical extraction
It takes in kettle, using mass ratio as 3:1 ethyl acetate and ethyl alcohol carries out supercritical CO for entrainer2Extraction 30min obtains no flower
Berry extract；The supercritical CO2The condition of extraction is：35 DEG C of extraction temperature, extracting pressure 40Mpa, CO2Flow velocity be
30L/min；The entrainer and the mass ratio of fig are 15:100.
Embodiment 3
A kind of Medium of Bifidobacterium containing Fructus Fici extract is made of the raw material of following mass concentration：Fig carries
Take object 42g/L, carbon source 22g/L, multi-vitamins 20mg/L, colostrum 55g/L, compound amino acid 12g/L, potassium dihydrogen phosphate
3g/L, magnesium chloride 0.5g/L, calcium chloride 0.2g/L, zinc sulfate 0.3g/L, frerrous chloride 0.3g/L, pH value 6.3；Wherein, no flower
The mass percentage of fruit polysaccharide is not less than 80%, remaining is alkaloid and glucoside；The molecular weight of the fig polysaccharide is not
Higher than 5000；The carbon source is glucose, sucrose and galactolipin, and the compound amino acid is by lysine, threonine, bright ammonia
Acid, isoleucine, valine, methionine, tryptophan, phenylalanine, cystine, cysteine, glycine, histidine, paddy ammonia
Acid, arginine composition；The multi-vitamins are by vitamin A, vitamin C, vitamin B1, vitamin B12, vitamin E group
Into
Extract obtains the Fructus Fici extract by the following method：It is the microwave of 400W by fig to use microwave power
Drying to percent water is 10%, then fig crushed 200 mesh sieves, and the fig after crushing is placed in overcritical extraction
It takes in kettle, using mass ratio as 3:1 ethyl acetate and ethyl alcohol carries out supercritical CO for entrainer2Extraction 35min obtains no flower
Berry extract；The supercritical CO2The condition of extraction is：35 DEG C of extraction temperature, extracting pressure 40Mpa, CO2Flow velocity be
30L/min；The entrainer and the mass ratio of fig are 12:100.
In order to illustrate the technique effect of the present invention, following control group is designed：
Control group 1
Control group 1 and the Medium of Bifidobacterium of embodiment 1 are essentially identical, and difference lies in the culture medium of control group 1 not
Contain Fructus Fici extract.
Control group 2
Control group 2 and the Medium of Bifidobacterium of embodiment 1 are essentially identical, and difference lies in the culture medium of control group 2
Fructus Fici extract replaces with isomalto-oligosaccharide glucose.
Control group 3
Control group 3 and the Medium of Bifidobacterium of embodiment 1 are essentially identical, and difference lies in the culture medium of control group 3
Fructus Fici extract replaces with fructose oligosaccharides.
Control group 4
Control group 4 and the Medium of Bifidobacterium of embodiment 1 are essentially identical, and difference lies in the culture medium of control group 4
Fructus Fici extract replaces with sucrose oligosaccharide.
Control group 5
Control group 5 and the Medium of Bifidobacterium of embodiment 1 are essentially identical, and difference lies in the culture medium of control group 5
Fructus Fici extract replaces with cow's milk oligosaccharide.
Control group 6
Control group 6 and the Medium of Bifidobacterium of embodiment 1 are essentially identical, and difference lies in the culture medium of control group 6
Fructus Fici extract replaces soyabean oligosaccharides.
Control group 7
Control group 7 and the Medium of Bifidobacterium of embodiment 1 are essentially identical, and difference lies in the culture medium of control group 7
Fructus Fici extract replaces galactose oligosaccharides.
Bifidobacterium culture experiment
The bifidobacterium species for selecting the activation of TPY culture mediums lyophilized, and after culture 48h under conditions of 37 DEG C of anaerobism, press
Inoculum concentration is inoculated in the culture medium of 1~embodiment of embodiment 3 and 1~control group of control group 7 for 5% and cultivates 12h, using plum
Teller-support benefit 320pH meters, is measured the pH value of bacterium solution, is recorded in table 1, is divided using UV-2401 UV, visible lights at room temperature
Photometer colorimetric under the conditions of 600nm measures the light absorption value of bacterium solution, is recorded in table 2；Bacterium solution is measured using method of dilution butteron on plate
Viable count, specific assay method are：After 1~embodiment of embodiment 3 and the bacterium solution of 1~control group of control group 7 are diluted 10 times,
Dilution is coated on TPY culture mediums, number of bacteria is calculated after 37 DEG C of Anaerobic culturel 12h, is as a result recorded in table 3.
Table 1pH values
Table 2OD600
3 viable count of table
From table 1, table 2, table 3, pH value：1~embodiment of embodiment, 3 2~control group of ＜ control groups, 7 ＜ control groups 1；
OD600：1~embodiment of embodiment, 3 2~control group of ＞ control groups, 7 ＞ control groups 1；Viable count：3 ＞ pairs of 1~embodiment of embodiment
According to group 2~control group, 7 ＞ control groups 1, the lower explanation of pH value, the carbon source in culture medium is good, and Bifidobacterium is to the utilization rate of carbon source
Height is bred；OD600It is higher with viable count, illustrate that the reproductive effect of Bifidobacterium is good.Thus, the Bifidobacterium of the present invention
It is good to the cultivation effect of Bifidobacterium that culture medium contains Fructus Fici extract.
Above description is the detailed description for the present invention preferably possible embodiments, but embodiment is not limited to this hair
Bright patent claim, the equal variation or modification change completed under the technical spirit suggested by all present invention, should all belong to
Cover the scope of the claims in the present invention.
Claims (7)
1. a kind of Medium of Bifidobacterium containing Fructus Fici extract, which is characterized in that its by following mass concentration raw material
Composition：40~50g/L of Fructus Fici extract, 20~30g/L of carbon source, 10~30mg/L of multi-vitamins, 50~60g/ of colostrum
L, 10~15g/L of compound amino acid, 2~5g/L of potassium dihydrogen phosphate, 0.1~1g/L of magnesium chloride, 0.1~0.3g/L of calcium chloride, sulphur
Sour 0.2~0.4g/L of zinc, 0.1~0.3g/L of frerrous chloride, pH value 6.2~6.5；
Extract obtains the Fructus Fici extract by the following method：It is 10% by fig drying to percent water, then
Fig be crushed into 200 mesh sieves, the fig after crushing is placed in supercritical extraction reactor, using mass ratio as 3:1 second
Acetoacetic ester and ethyl alcohol carry out supercritical CO for entrainer230~40min of extraction obtains Fructus Fici extract；The supercritical CO2
The condition of extraction is：35 DEG C of extraction temperature, extracting pressure 40Mpa, CO2Flow velocity be 30L/min；The entrainer and no flower
The mass ratio of fruit is 10~15:100.
2. a kind of Medium of Bifidobacterium containing Fructus Fici extract according to claim 1, which is characterized in that described
Fructus Fici extract in, not less than 80%, remaining is alkaloid and glucoside for the mass percentage of fig polysaccharide.
3. a kind of Medium of Bifidobacterium containing Fructus Fici extract according to claim 2, which is characterized in that described
Fig polysaccharide molecular weight be not higher than 5000.
4. a kind of Medium of Bifidobacterium containing Fructus Fici extract according to claim 1, which is characterized in that described
Carbon source for glucose, sucrose, the one or more of galactolipin.
5. a kind of Medium of Bifidobacterium containing Fructus Fici extract according to claim 1, which is characterized in that described
Compound amino acid by lysine, threonine, leucine, isoleucine, valine, methionine, tryptophan, phenylalanine, Guang
Propylhomoserin, cysteine, glycine, histidine, glutamic acid, arginine composition.
6. a kind of Medium of Bifidobacterium containing Fructus Fici extract according to claim 1, which is characterized in that described
Multi-vitamins be made of vitamin A, vitamin C, vitamin B1, vitamin B12, vitamin E.
7. a kind of Medium of Bifidobacterium containing Fructus Fici extract according to claim 1, which is characterized in that described
Fig drying dried using microwave drying mode；The microwave power is 300~500W.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113943676A (en) * | 2021-10-29 | 2022-01-18 | 济南紫金玫瑰股份有限公司 | Culture medium of bacterial liquid |
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| WO2010019413A2 (en) * | 2008-08-12 | 2010-02-18 | Elc Management Llc | Composite particles having an antioxidant-based protective system, and topical compositions comprising the same |
| CN102766590A (en) * | 2012-07-09 | 2012-11-07 | 陕西科技大学 | Streptococcus thermophilus enrichment medium and preparation method thereof |
| CN105949337A (en) * | 2016-05-13 | 2016-09-21 | 福建农林大学 | Method for extracting polysaccharide capable of promoting bifidobacterium multiplication from fresh tremella |
| CN107080258A (en) * | 2017-04-11 | 2017-08-22 | 姜红成 | Prepare the fermentation composition and preparation method of the plant enzyme with conditioning collaterals of human rich in nutrition function |
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Patent Citations (5)
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|---|---|---|---|---|
| CN1584013A (en) * | 2004-05-31 | 2005-02-23 | 河北农业大学 | Composite cultural media for fermentation of bifidobacteruim and its preparation |
| WO2010019413A2 (en) * | 2008-08-12 | 2010-02-18 | Elc Management Llc | Composite particles having an antioxidant-based protective system, and topical compositions comprising the same |
| CN102766590A (en) * | 2012-07-09 | 2012-11-07 | 陕西科技大学 | Streptococcus thermophilus enrichment medium and preparation method thereof |
| CN105949337A (en) * | 2016-05-13 | 2016-09-21 | 福建农林大学 | Method for extracting polysaccharide capable of promoting bifidobacterium multiplication from fresh tremella |
| CN107080258A (en) * | 2017-04-11 | 2017-08-22 | 姜红成 | Prepare the fermentation composition and preparation method of the plant enzyme with conditioning collaterals of human rich in nutrition function |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN113943676A (en) * | 2021-10-29 | 2022-01-18 | 济南紫金玫瑰股份有限公司 | Culture medium of bacterial liquid |
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