CN108102937A - A kind of yeast culture and preparation method thereof, application - Google Patents
A kind of yeast culture and preparation method thereof, application Download PDFInfo
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/16—Yeasts; Culture media therefor
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/33—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from molasses
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/189—Enzymes
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/24—Compounds of alkaline earth metals, e.g. magnesium
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/26—Compounds containing phosphorus
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/145—Fungal isolates
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
- C12P1/02—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes by using fungi
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/645—Fungi ; Processes using fungi
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
Abstract
A kind of yeast culture and preparation method thereof, application, are related to yeast cells fermentation technical field.The present invention proposes a kind of preparation method of yeast culture, including：Saccharomycete is subjected to actication of culture, the strain of activation is seeded in culture in liquid seed culture medium obtains fermentation seed liquid；Fermentation seed liquid inoculation is carried out to first time liquid aerobic fermentation, liquid anaerobic fermentation and second of liquid aerobic fermentation successively in liquid medium, obtains liquid culture；Liquid culture is seeded in progress solid anaerobic fermentation in solid medium and obtains solid fermentation object；It is dried after solid fermentation object is mixed progress anaerobism self-dissolving with exogenous enzymes.The preparation method can improve the bacterium number content and active material of yeast culture, improve the quality of yeast culture.
Description
Technical field
The present invention relates to yeast cells fermentation technical field, and more particularly to a kind of yeast culture and preparation method thereof,
Using.
Background technology
Yeast is unicellular eukaryote, in vivo containing a variety of nutrients such as abundant protein, amino acid and nucleotide
Matter, wherein protein content are up to 50% of its dry matter or so, containing eight kinds of amino acid needed by human, and amino acid ratio
The ideal amino acid composition ratio that the very close FAO (Food and Agriculture Organization of the United Nation) of example is recommended.Yeast culture is a kind of probiotics production
Product refer to by yeast-inoculated among specific culture substrate, carry out the Tiny ecosystem product that aerobic or anaerobic fermentation is formed.
The various active substance that yeast culture is contained by it can adjust host immune function, the battalion for improving animal intestinal tract
It supports, optimization intestinal eubiosis, absorption of the animal intestinal tract to nutriment can be greatly enhanced, reduce feedstuff-meat ratio, improve animal
Production performance, reduce antibiotic usage, culture benefit is greatly improved, preserves the ecological environment.
Yeast culture product is more on Vehicles Collected from Market, and the quality of product is mainly determined by the reasonability of manufacture craft.When
The production technology of preceding yeast culture mainly has two kinds of solution fermentation and solid fermentation method.Liquid fermentation has production technology letter
Single, the big feature of yield, but there are the shortcomings of of high cost, easy to pollute, metabolite loss is excessive.Solid fermentation process then directly will
Zymotic fluid is inoculated on solid medium, and gained tunning includes all thalline, metabolite and nutrient media components, zero-emission
It puts, containing abundant nutritional ingredient, environmental pollution is small.Meanwhile solid fermentation raw materials are various, derive from a wealth of sources, and can effectively subtract
Few fermentation costs.
The content of the invention
The first object of the present invention is to provide a kind of preparation method of yeast product, which can improve yeast
The bacterium number content and active material of culture improve the quality of yeast culture.
The second object of the present invention is to provide a kind of yeast product have the characteristics that quality is good, effect is strong.
The third object of the present invention is that providing a kind of yeast product is preparing the application of feed addictive.
The present invention is solved its technical problem and is realized using following technical scheme.
The present invention proposes a kind of preparation method of yeast culture, including：
Saccharomycete is subjected to actication of culture, the strain of activation is seeded in culture in liquid seed culture medium obtains fermentation kind
Sub- liquid；
Fermentation seed liquid inoculation is carried out to first time liquid aerobic fermentation, liquid anaerobic fermentation successively in liquid medium
With second of liquid aerobic fermentation, liquid culture is obtained；
Liquid culture is seeded in progress solid anaerobic fermentation in solid medium and obtains solid fermentation object；
It is dried after solid fermentation object is mixed progress anaerobism self-dissolving with exogenous enzymes.
A kind of yeast culture, yeast culture are made by the preparation method of above-mentioned yeast culture.
A kind of yeast culture described above is preparing the application of feed addictive.
The advantageous effect of the embodiment of the present invention is：The preparation method of the yeast culture of the disclosure, using first time liquid
Aerobic fermentation, liquid anaerobic fermentation and second of liquid aerobic fermentation, yeast culture bacterium number height, the metabolite content of gained
It is high；Liquid fermentation process with solid fermentation process is combined, the generation generated during liquid fermentation can be retained to greatest extent
It thanks to product, reduces the loss of yeast culture nutrition；The yeast autolysis stage by the way of anaerobism+exogenous enzymes, can fully allow
Yeast autolysis discharges active material, greatly improves the validity of yeast culture nutriment.By made from the preparation method
Yeast culture has the characteristics that quality is good, effect is strong.
Specific embodiment
It, below will be in the embodiment of the present invention to make the purpose, technical scheme and advantage of the embodiment of the present invention clearer
Technical solution be clearly and completely described.The person that is not specified actual conditions in embodiment, builds according to normal condition or manufacturer
The condition of view carries out.Reagents or instruments used without specified manufacturer is the conventional production that can be obtained by commercially available purchase
Product.
Below to a kind of yeast culture of the embodiment of the present invention and preparation method thereof, using being specifically described.
A kind of preparation method of yeast culture, including：
Saccharomycete is subjected to actication of culture, the strain of activation is seeded in culture in liquid seed culture medium obtains fermentation kind
Sub- liquid.
Wherein, saccharomycete using oese is inoculated in slant medium and carried out by the actication of culture of saccharomycete.Into
One step, slant medium is PDA solid mediums.I.e. PDA solid mediums are by potato 200g, glucose 20g and fine jade
15~20g of cosmetics adds water to be settled to 1000mL to be made.
In the present embodiment, the temperature of actication of culture is 28-30 DEG C, soak time 1-3d.
In addition, in the present embodiment, saccharomycete is S. cervisiae.S. cervisiae is from applicant from natural environment
Middle screening is isolated, has the characteristics that growth activity is high, production metabolite is abundant.It should be noted that saccharomycete can also
It is bread microzyme.In addition, saccharomycete also can be by being directly commercially available.
Further, in the present embodiment, liquid seed culture medium is malt extract medium.Malt extract medium be by
Malt extract powder 150g, agar 20g and chloramphenicol 0.1g add water to be settled to 1000mL and are made.In addition, in the present embodiment,
It it is 28-30 DEG C, incubation time 1-3d, rotating speed 160-180rpm in the temperature of liquid seed culture medium culture.
Fermentation seed liquid inoculation is carried out to first time liquid aerobic fermentation, liquid anaerobic fermentation successively in liquid medium
With second of liquid aerobic fermentation, liquid culture is obtained.
By first time liquid aerobic fermentation, biological oxidation occurs for the effective active in fermentation seed liquid, is detested by liquid
Aerobe fermentation, the organic matter in fermentation seed liquid decomposes, then carries out second of liquid aerobic fermentation, and biological oxidation further occurs.
Using first time liquid aerobic fermentation, liquid anaerobic fermentation and second of liquid aerobic fermentation, the yeast culture bacterium number of gained
High, yield height, metabolite content are high.
Wherein, fluid nutrient medium includes by weight percentage：Molasses 4-6%, corn flour 0.35-0.45%, ammonium sulfate
0.5-0.7%, potassium dihydrogen phosphate 0.05-0.15%, the water of magnesium sulfate 0.05-0.15% and surplus；Preferably, fluid nutrient medium
PH be 7-7.2.Fluid nutrient medium in the pH value range is more advantageous to fermentation seed liquid and ferments.
Further, in some embodiments, first time liquid aerobic fermentation be temperature be 28-30 DEG C, throughput
Fermented and cultured 1-3d under conditions of being 110-150rpm for 1.0-1.5vvm, rotating speed.In some embodiments, liquid anaerobic is sent out
Ferment is the anaerobic fermentation 1-2d under conditions of temperature is 28-30 DEG C.In some embodiments, second of liquid aerobic fermentation is
The fermented and cultured 1-3d under conditions of temperature is 28-30 DEG C, throughput 1.0-1.5vvm, rotating speed are 110-150rpm.
Further, in the present embodiment, the terminal of first time liquid aerobic fermentation reaches 2 × 10 for bacterium number9CFU/
More than mL, pH are less than 4.5.In the present embodiment, the terminal of liquid anaerobic fermentation is bacterium number 5 × 109More than CFU/mL, pH
For less than 4.0.In the present embodiment, the terminal of second of liquid aerobic fermentation reaches 6 × 10 for bacterium number9More than CFU/g, pH
For less than 4.0.In the present embodiment, the terminal of solid anaerobic fermentation reaches 6 × 10 for bacterium number9More than CFU/g, pH 4.0
Below.
Liquid culture is seeded in progress solid anaerobic fermentation in solid medium and obtains solid fermentation object.
Wherein, solid medium count in parts by weight including：40-60 parts of corn flour, 10-30 parts of wheat bran and dregs of beans 10-
30 parts.In the present embodiment, solid anaerobic fermentation is the 1-3d that ferments under conditions of temperature is 30-40 DEG C.When temperature is higher than
, it is necessary to carry out turning over throwing to fermentation material at 40 DEG C.
Present embodiment can retain liquid to greatest extent by the way that liquid fermentation process is combined with solid fermentation process
The metabolite generated in fermentation process reduces the loss of yeast culture nutrition.
After solid fermentation object is mixed progress anaerobism self-dissolving with exogenous enzymes, it is dried.
Wherein, the step of exogenous enzymes are papain, anaerobism self-dissolving includes：Solid fermentation object is pressed with papain
It is 100 according to weight ratio:0.1~0.5 mixing is 48-52 DEG C, causes yeast autolysis under conditions of anaerobism in temperature.
The yeast autolysis stage by the way of anaerobism+exogenous enzymes, can fully allow yeast autolysis, discharge active material, greatly
The big validity for improving yeast culture nutriment.
Further, in the present embodiment, the process of anaerobism self-dissolving is carried out in the woven bag of sealed air-tight,
The solvent of woven bag is 30L.
Further, in the present embodiment, dry temperature is 40-45 DEG C.Yeast culture is subjected to powder after drying
It is broken to obtain the yeast culture of powdery.By way of low temperature drying crushing, drying and crushing can be reduced in the process to active material
Caused by destruction.
A kind of yeast culture as made from above-mentioned preparation method, with the spy that quality is good, stability is good, effect is strong
Point.
A kind of yeast culture described above is preparing the application of feed addictive.The yeast culture is added as feed
Agent can be effectively improved intestinal eubiosis, promote intestinal absorption, improve breeding performonce fo animals, have in livestock and poultry cultivation wide
Wealthy application prospect.
The feature and performance of the present invention are described in further detail with reference to embodiments.
Embodiment 1
The present embodiment provides a kind of yeast culture, the preparation method of the yeast culture comprises the following steps：
(1) saccharomyces cerevisiae strain is inoculated in oese in slant medium, is carried out under conditions of temperature is 28 DEG C
Actication of culture cultivates 1d.Wherein slant medium is by potato 200g, glucose 20g and agar powder 20g water to be added to be settled to
1000mL is made.
(2) strain of activation is seeded in liquid seed culture medium, is 28 DEG C in temperature, rotating speed is the condition of 160rpm
Lower culture 1d obtains fermentation seed liquid.Wherein, liquid seed culture medium is by malt extract powder 150g, agar 20g and chloramphenicol
0.1g adds water to be settled to 1000mL and is made.
(3) fermentation seed liquid inoculation is subjected to first time liquid aerobic fermentation, liquid anaerobic successively in liquid medium
Fermentation and second of liquid aerobic fermentation, obtain liquid culture.
Wherein, first time liquid aerobic fermentation is in the item that temperature is 28 DEG C, throughput 1.0vvm, rotating speed are 110rpm
Fermented and cultured 1d under part reaches 2 × 10 with bacterium number9It less than 4.5 is terminal that more than CFU/mL, pH, which are,.Liquid anaerobic fermentation be
Temperature is anaerobic fermentation 1d under conditions of 28 DEG C, with bacterium number 5 × 109It less than 4.0 is terminal that more than CFU/mL, pH, which are,.Second
Liquid aerobic fermentation is the fermented and cultured 1d under conditions of temperature is 28 DEG C, throughput 1.0vvm, rotating speed are 110rpm, with bacterium
Number reaches 6 × 109It less than 4.0 is terminal that more than CFU/g, pH, which are,.
Fluid nutrient medium includes by weight percentage：Molasses 4%, corn flour 0.35%, ammonium sulfate 0.5%, di(2-ethylhexyl)phosphate
The water of hydrogen potassium 0.05%, magnesium sulfate 0.05% and surplus, the pH of fluid nutrient medium is 7.
(4) liquid culture is seeded in solid medium, solid anaerobic fermentation 1d is carried out under conditions of 30 DEG C, it is high
Fermentation material is carried out when 40 DEG C to turn over throwing, obtains solid fermentation object.
Wherein, solid medium is：40 parts by weight of corn flour, 30 parts by weight of wheat bran, 30 parts by weight of dregs of beans.
(5) it is 100 according to weight ratio by solid fermentation object and papain:0.1 mixing, is packed into 30L sealed air-tights
Woven bag in, anaerobism self-dissolving 1d is carried out at a temperature of 50 DEG C, then dry under conditions of 40 DEG C, then crushing must make
Product.
Embodiment 2
The present embodiment provides a kind of yeast culture, the preparation method of the yeast culture comprises the following steps：
(1) saccharomyces cerevisiae strain is inoculated in oese in slant medium, is carried out under conditions of temperature is 30 DEG C
Actication of culture cultivates 2d.Wherein slant medium is by potato 200g, glucose 20g and agar powder 20g water to be added to be settled to
1000mL is made.
(2) strain of activation is seeded in liquid seed culture medium, is 30 DEG C in temperature, rotating speed is the condition of 170rpm
Lower culture 2d obtains fermentation seed liquid.Wherein, liquid seed culture medium is by malt extract powder 150g, agar 20g and chloramphenicol
0.1g adds water to be settled to 1000mL and is made.
(3) fermentation seed liquid inoculation is subjected to first time liquid aerobic fermentation, liquid anaerobic successively in liquid medium
Fermentation and second of liquid aerobic fermentation, obtain liquid culture.
Wherein, first time liquid aerobic fermentation is in the item that temperature is 29 DEG C, throughput 1.3vvm, rotating speed are 130rpm
Fermented and cultured 2d under part reaches 2 × 10 with bacterium number9It less than 4.5 is terminal that more than CFU/mL, pH, which are,.Liquid anaerobic fermentation be
Temperature is anaerobic fermentation 1.5d under conditions of 29 DEG C, with bacterium number 5 × 109It less than 4.0 is terminal that more than CFU/mL, pH, which are,.Second
Secondary liquid aerobic fermentation is the fermented and cultured 2d under conditions of temperature is 29 DEG C, throughput 1.3vvm, rotating speed are 130rpm, with
Bacterium number reaches 6 × 109It less than 4.0 is terminal that more than CFU/g, pH, which are,.
Fluid nutrient medium includes by weight percentage：Molasses 5%, corn flour 0.4%, ammonium sulfate 0.6%, biphosphate
The water of potassium 0.1%, magnesium sulfate 0.1% and surplus, the pH of fluid nutrient medium is 7.1.
(4) liquid culture is seeded in solid medium, solid anaerobic fermentation 3d is carried out under conditions of 35 DEG C, it is high
Fermentation material is carried out when 40 DEG C to turn over throwing, obtains solid fermentation object.
Wherein, solid medium is：50 parts by weight of corn flour, 20 parts by weight of wheat bran, 20 parts by weight of dregs of beans.
(5) it is 100 according to weight ratio by solid fermentation object and papain:0.3 mixing, is packed into 30L sealed air-tights
Woven bag in, anaerobism self-dissolving 1.5d is carried out at a temperature of 50 DEG C, then dry under conditions of 43 DEG C, then crushing must make
Product.
Embodiment 3
The present embodiment provides a kind of yeast culture, the preparation method of the yeast culture comprises the following steps：
(1) saccharomyces cerevisiae strain is inoculated in oese in slant medium, is carried out under conditions of temperature is 29 DEG C
Actication of culture cultivates 3d.Wherein slant medium is by potato 200g, glucose 20g and agar powder 20g water to be added to be settled to
1000mL is made.
(2) strain of activation is seeded in liquid seed culture medium, is 29 DEG C in temperature, rotating speed is the condition of 180rpm
Lower culture 3d obtains fermentation seed liquid.Wherein, liquid seed culture medium is by malt extract powder 150g, agar 20g and chloramphenicol
0.1g adds water to be settled to 1000mL and is made.
(3) fermentation seed liquid inoculation is subjected to first time liquid aerobic fermentation, liquid anaerobic successively in liquid medium
Fermentation and second of liquid aerobic fermentation, obtain liquid culture.
Wherein, first time liquid aerobic fermentation is in the item that temperature is 30 DEG C, throughput 1.0vvm, rotating speed are 150rpm
Fermented and cultured 3d under part reaches 2 × 10 with bacterium number9It less than 4.5 is terminal that more than CFU/mL, pH, which are,.Liquid anaerobic fermentation be
Temperature is anaerobic fermentation 2d under conditions of 30 DEG C, with bacterium number 5 × 109It less than 4.0 is terminal that more than CFU/mL, pH, which are,.Second
Liquid aerobic fermentation is the fermented and cultured 3d under conditions of temperature is 30 DEG C, throughput 1.5vvm, rotating speed are 150rpm, with bacterium
Number reaches 6 × 109It less than 4.0 is terminal that more than CFU/g, pH, which are,.
Fluid nutrient medium includes by weight percentage：Molasses 6%, corn flour 0.45%, ammonium sulfate 0.7%, di(2-ethylhexyl)phosphate
The water of hydrogen potassium 0.15%, magnesium sulfate 0.15% and surplus, the pH of fluid nutrient medium is 7.2.
(4) liquid culture is seeded in solid medium, solid anaerobic fermentation 1d is carried out under conditions of 40 DEG C, it is high
Fermentation material is carried out when 40 DEG C to turn over throwing, obtains solid fermentation object.
Wherein, solid medium is：60 parts by weight of corn flour, 30 parts by weight of wheat bran, 30 parts by weight of dregs of beans.
(5) it is 100 according to weight ratio by solid fermentation object and papain:0.5 mixing, is packed into 30L sealed air-tights
Woven bag in, anaerobism self-dissolving is carried out at a temperature of 52 DEG C, it is then dry under conditions of 45 DEG C, then crush to obtain product.
Embodiment 4
The present embodiment provides a kind of yeast culture, the preparation method of the yeast culture comprises the following steps：
(1) saccharomyces cerevisiae strain is inoculated in oese in slant medium, is carried out under conditions of temperature is 29 DEG C
Actication of culture cultivates 2d.Wherein slant medium is by potato 200g, glucose 20g and agar powder 15g water to be added to be settled to
1000mL is made.
(2) strain of activation is seeded in liquid seed culture medium, is 30 DEG C in temperature, rotating speed is the condition of 170rpm
Lower culture 1d obtains fermentation seed liquid.Wherein, liquid seed culture medium is by malt extract powder 150g, agar 20g and chloramphenicol
0.1g adds water to be settled to 1000mL and is made.
(3) fermentation seed liquid inoculation is subjected to first time liquid aerobic fermentation, liquid anaerobic successively in liquid medium
Fermentation and second of liquid aerobic fermentation, obtain liquid culture.
Wherein, first time liquid aerobic fermentation is in the item that temperature is 30 DEG C, throughput 1.0vvm, rotating speed are 110rpm
Fermented and cultured 3d under part reaches 2 × 10 with bacterium number9It less than 4.5 is terminal that more than CFU/mL, pH, which are,.Liquid anaerobic fermentation be
Temperature is anaerobic fermentation 2d under conditions of 30 DEG C, with bacterium number 5 × 109It less than 4.0 is terminal that more than CFU/mL, pH, which are,.Second
Liquid aerobic fermentation is the fermented and cultured 3d under conditions of temperature is 30 DEG C, throughput 1.5vvm, rotating speed are 110rpm, with bacterium
Number reaches 6 × 109It less than 4.0 is terminal that more than CFU/g, pH, which are,.
Fluid nutrient medium includes by weight percentage：Molasses 5%, corn flour 0.45%, ammonium sulfate 0.6%, di(2-ethylhexyl)phosphate
The water of hydrogen potassium 0.1%, magnesium sulfate 0.15% and surplus, the pH of fluid nutrient medium is 7.2.
(4) liquid culture is seeded in solid medium, solid anaerobic fermentation 1d is carried out under conditions of 30 DEG C, it is high
Fermentation material is carried out when 40 DEG C to turn over throwing, obtains solid fermentation object.
Wherein, solid medium is：50 parts by weight of corn flour, 10 parts by weight of wheat bran, 10 parts by weight of dregs of beans.
(5) it is 100 according to weight ratio by solid fermentation object and papain:0.5 mixing, is packed into 30L sealed air-tights
Woven bag in, anaerobism self-dissolving is carried out at a temperature of 48 DEG C, it is then dry under conditions of 40 DEG C, then crush to obtain product.
Comparative example 1
The preparation method of the yeast culture of comparative example 1 and the preparation method of the yeast culture of embodiment 1 are essentially identical,
It the difference is that only in the step of comparative example 1 (4) that solid medium is：70 parts by weight of corn flour, 20 parts by weight of wheat bran,
10 parts by weight of dregs of beans, the temperature of solid anaerobic fermentation is 50 DEG C, time 5d, and fermentation material is carried out when higher than 45 DEG C to turn over throwing.
Comparative example 2
The preparation method of the yeast culture of comparative example 1 and the preparation method of the yeast culture of embodiment 2 are essentially identical,
It the difference is that only in the step of comparative example 2 (5) that solid fermentation object is 100 according to weight ratio with papain:0.1
Mixing, dry temperature are 55 DEG C.
Test example
The yeast culture that embodiment 1-3 and comparative example 1-2 are prepared carries out animal experiment, takes 4 week old or so, puts down
Equal weight amounts to 60 for the weanling pig of 7.5kg or so, and every 10 weanling pigs are divided into one group, amounts to 6 groups, respectively tests
Group 1-5 and blank group, the feed that experimental group 1-5 is searched for food is respectively added with embodiment 1, embodiment 2, embodiment 3, comparative example
1 and comparative example 2 yeast culture feed, the above-mentioned corresponding yeast cultures of 5kg are added in feed per ton, blank group is adopted
The no added yeast culture of feed of food, the other compositions of feed can be identical, and the wean of determination experiment group 1-5 and blank group is young
The daily ingestion amount of pig, test data refer to table 1.
The 1 weanling pig daily ingestion amount data table of comparisons of table
The test result of table 1 is referred to, the Yeast Cultivation of the embodiment of the present invention is can be seen that from the data of experimental group 1-3
After object is added in the daily ration of wean newborn pig, from experiment the 4th day, the average daily gain of weanling pig 0.60kg with
On, with the increase of feeding age in days, daily ingestion amount increases therewith, when age in days reaches 36d, the highest average daily ingestion amount of piglet
It is minimum also up to 1.29kg up to 1.36kg.
The feed intake that can be seen that experimental group 1-3 by the result of contrast experiment group 1-3 and experimental group 4-5, blank group is equal
Higher than 4-5 and blank group.The feed intake of experimental group 4-5 and blank group no significant difference.The embodiment of the present invention is shown to be made
Yeast culture can significantly improve the daily ingestion amount of piglet, there is significant facilitation for the growth of piglet.
Embodiments described above is part of the embodiment of the present invention, instead of all the embodiments.The reality of the present invention
The detailed description for applying example is not intended to limit the scope of claimed invention, but is merely representative of the selected implementation of the present invention
Example.Based on the embodiments of the present invention, those of ordinary skill in the art are obtained without creative efforts
Every other embodiment, belongs to the scope of protection of the invention.
Claims (10)
1. a kind of preparation method of yeast culture, which is characterized in that including：
Saccharomycete is subjected to actication of culture, the strain of activation is seeded in culture in liquid seed culture medium obtains fermentation seed
Liquid；
Fermentation seed liquid inoculation is carried out to first time liquid aerobic fermentation, liquid anaerobic fermentation successively in liquid medium
With second of liquid aerobic fermentation, liquid culture is obtained；
The liquid culture is seeded in progress solid anaerobic fermentation in solid medium and obtains solid fermentation object；
After the solid fermentation object is mixed progress anaerobism self-dissolving with exogenous enzymes, it is dried.
2. the preparation method of yeast culture according to claim 1, which is characterized in that the actication of culture of the saccharomycete
The saccharomycete is inoculated in slant medium using oese and is carried out；Preferably, the slant medium is consolidated for PDA
Body culture medium；The temperature of the actication of culture is 28-30 DEG C, soak time 1-3d.
3. the preparation method of yeast culture according to claim 1, which is characterized in that the liquid seed culture medium is
Malt extract medium is 28-30 DEG C in the temperature of the liquid seed culture medium culture, incubation time 1-3d.
4. the preparation method of yeast culture according to claim 1, which is characterized in that the fluid nutrient medium is by weight
Percentage includes：Molasses 4-6%, corn flour 0.35-0.45%, ammonium sulfate 0.5-0.7%, potassium dihydrogen phosphate 0.05-0.15%,
The water of magnesium sulfate 0.05-0.15% and surplus；Preferably, the pH of the fluid nutrient medium is 7-7.2.
5. the preparation method of yeast culture according to claim 1, which is characterized in that the aerobic hair of first time liquid
Ferment is the fermented and cultured 1-3d under conditions of temperature is 28-30 DEG C, throughput is 1.0-1.5vvm；Preferably, the liquid is detested
Aerobe fermentation is the anaerobic fermentation 1-2d under conditions of temperature is 28-30 DEG C；Preferably, second of the liquid aerobic fermentation be
Fermented and cultured 1-3d under conditions of temperature is 28-30 DEG C, throughput is 1.0-1.5vvm.
6. the preparation method of yeast culture according to claim 1, which is characterized in that the aerobic hair of first time liquid
The terminal of ferment reaches 2 × 10 for bacterium number9More than CFU/mL, pH are less than 4.5；Preferably, the terminal of the liquid anaerobic fermentation
For bacterium number 5 × 109More than CFU/mL, pH are less than 4.0；Preferably, the terminal of second of the liquid aerobic fermentation is bacterium number
Reach 6 × 109More than CFU/g, pH are less than 4.0；Preferably, the terminal of the solid anaerobic fermentation reach 6 for bacterium number ×
109More than CFU/g, pH are less than 4.0.
7. the preparation method of yeast culture according to claim 1, which is characterized in that the solid medium is by weight
Number meter includes：10-30 parts of 40-60 parts of corn flour, 10-30 parts of wheat bran and dregs of beans；Preferably, the solid anaerobic fermentation is
Ferment 1-3d under conditions of temperature is 30-40 DEG C.
8. the preparation method of yeast culture according to claim 1, which is characterized in that the exogenous enzymes are Papain
The step of enzyme, the anaerobism self-dissolving, includes：According to weight ratio it is 100 by the solid fermentation object and the papain:0.1
~0.5 mixing is 48-52 DEG C, causes yeast autolysis under conditions of anaerobism in temperature.
9. a kind of yeast culture, which is characterized in that the yeast culture is trained by claim 1-8 any one of them yeast
The preparation method for supporting object is made.
10. a kind of yeast culture as claimed in claim 9 is preparing the application of feed addictive.
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| CN111334407A (en) * | 2020-03-16 | 2020-06-26 | 高唐华农生物工程有限公司 | Process technology and process equipment for preparing yeast culture by deep fermentation of yeast |
| CN115010636A (en) * | 2022-06-30 | 2022-09-06 | 山东微研生物科技有限公司 | Extraction method of cantharis yellow fermentation liquid |
| CN115261244A (en) * | 2022-06-30 | 2022-11-01 | 山东微研生物科技有限公司 | Culture medium composition and fermentation process for high-yield canthaxanthin production by yarrowia lipolytica |
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| CN111334407B (en) * | 2020-03-16 | 2023-06-23 | 高唐华农生物工程有限公司 | Process technology and process equipment for preparing yeast culture by deep fermentation of yeast |
| CN115010636A (en) * | 2022-06-30 | 2022-09-06 | 山东微研生物科技有限公司 | Extraction method of cantharis yellow fermentation liquid |
| CN115261244A (en) * | 2022-06-30 | 2022-11-01 | 山东微研生物科技有限公司 | Culture medium composition and fermentation process for high-yield canthaxanthin production by yarrowia lipolytica |
| CN115261244B (en) * | 2022-06-30 | 2024-02-23 | 山东微研生物科技有限公司 | Culture medium combination and fermentation process for high-yield canthaxanthin by utilizing yarrowia lipolytica |
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Application publication date: 20180601 |