CN108096355B - 一种治疗猪寒湿型泄泻中药复方组方、制备及应用 - Google Patents
一种治疗猪寒湿型泄泻中药复方组方、制备及应用 Download PDFInfo
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Abstract
本发明涉及一种治疗猪寒湿型腹泻中药复方组方、制备及应用,属于中兽药制备技术领域。将石榴皮、广藿香、吴茱萸按一定比例组合;广藿香、吴茱萸加适量石油醚超声提取挥发油并加入适量酒精溶解后待用;药渣挥发净溶剂后合并石榴皮,加入15倍馏水,反复煎煮2次,煎液合并,用4层纱布过滤,滤液隔水浓缩后加入上述挥发油,充分摇匀,分装即得。该复方不仅对寒湿型泄泻大鼠模型有显著的治疗作用,治愈寒湿型泄泻临床自然发病仔猪的疗程较临床常用中西对照药物均缩短1~2天,显著降低发病仔猪腹泻指数和腹泻复发率。本发明所获中药复方不仅对寒湿型泄泻仔猪有较好的治疗效果,防止复发,且组方和制备工艺及质控方法简单,使用方法简便。
Description
一、技术领域
本发明涉及一种治疗猪寒湿型泄泻中药复方组方、制备及应用,属于中兽药制备技术领域。
二、背景技术
泄泻,是指排粪次数增多、粪便稀薄,甚至泄粪如水样。泄泻是兽医临床最常见病症,表现形式多样,原因复杂,危害严重,发病率,涉及不同年龄的各种家养动物,是严重危害养殖业最主要病症之一,给养殖业造成了极大的经济损失。泄泻的主要病位在于脾胃及大小肠,其他如肾阳不足,也可能导致脾胃功能失常,发生泄泻。引起泄泻的原因有很多,临床主要的原因有感受寒湿、热积胃肠、宿食停滞、脾胃虚弱等等。其中寒湿型泄泻是主要的一种证型,一般见于外感寒湿传于脾胃,或中焦伤于阴冷,运化无力,致使寒湿下注,清浊不分而成泄泻。
家养动物中,猪所居之地多湿,寒湿兼杂,更易侵袭猪体;且目前猪之食物,主要为淀粉、脂肪、蛋白等组成的干料,多厚腻而生痰湿,饮水量更达料的2-3倍,其所饮之水也为生冷之物。所以,生产中,几乎所有的养猪场都经常受困于这种类型的泄泻。尤其是在寒冷季节和多湿季节。
由于饲料和环境的不利因素始终持续存在,造成猪等动物的寒湿泄泻普遍发生,且在寒冷多湿季节尤其严重。目前在生产中对于这类疾病的主要应用广泛大剂量的抗生素进行治疗,但效果往往并不理想,生产中出现的明显现象就是一停药就又反复出现泄泻,用药后又逐渐好转。这种现象的根本原因在于未对该证候进行合理的辨证施药,临床也没有简便价廉效佳的药物。本证的根本原因并不在于微生物的感染,而在于寒湿侵袭。
因此,研发有效的治疗用药物,已经迫在眉睫。
三、发明内容
技术问题 本发明针对目前畜牧生产中动物寒湿泄泻的普遍发生并缺乏有效治疗用药,提供了一种治疗用中药复方,所获得的中药复方按照一定方法制备后形成的合剂,不仅组方简单、使用方法简便、成本低廉、无污染,而且对猪寒湿泄泻有良好的疗效,用药1-3次后治愈,连续观察1周未见复发,药效明显优于对照西药庆大霉素等,且不复发,可用于猪寒湿证的临床防治。
鉴于寒湿证泄泻病因为寒湿侵袭或集聚,其病理损伤主要为胃肠。中药治疗宜温中散寒、化湿止泻,理气止痛。因此,我们选用石榴皮、吴茱萸、广藿香组成复方。石榴皮是石榴科植物十六的干燥果皮,如大肠经;功能收敛止泻,对于虚寒所致久泄久痢等证具有良好效果;临床常用于腹泻的防治,本方中收敛止泻为主药。藿香为唇形花科植物广藿香的干燥茎叶,入脾胃肺经;功能芳香化湿、和中止痛、除湿滞;临床常用于湿浊内阻证候,本方中化湿除滞为辅药。吴茱萸为芸香科植物吴茱萸的干燥未成熟果实,入肝、肾、脾、胃经;功能温中止痛;常用于冷痛;本方中暖脾止痛为佐药。三药合用,温中散寒、化湿止泻,理气止痛,针对寒湿型泄泻的主证,配伍施治,发挥良效。
技术方案
中药石榴皮、广藿香、吴茱萸分别为主、辅、佐使药。按照石榴皮1000g、广藿香30g、吴茱萸20g;取广藿香、吴茱萸加6倍质量的石油醚,浸泡12h,调整超声仪温度40℃、功率220W、时间40min,超声震荡萃取,反复2次,合并萃取液,用旋转蒸发仪真空浓缩,挥发净溶剂后加入50ml无水酒精待用;广藿香、吴茱萸萃取后药渣挥发净溶剂后与石榴皮合并,加入15倍蒸馏水,浸泡40min后,武火煎煮迅速至沸腾,改为文火维持沸腾60分钟,收集的煎煮液;药渣再加15倍质量的水,同上法煎煮,收集并合并2次煎煮液,4层纱布过滤煎煮液,并隔水浓缩至950mL,加入酒精溶解的挥发油、苯甲酸钠15克,充分摇匀,分装入鸭嘴瓶,备用。对临床自然发病的10-22日龄寒湿泄泻证仔猪口腔喷灌2ml,每天1次,连续3天,并持续观察1周。每天统计动物腹泻率和腹泻评分,评价该复方对猪寒湿泄泻证的治疗效果。按此方法制备的中药复方为水溶性深褐色均匀液体,对猪寒湿泄泻证有较好的治疗效果,且组方和制备工艺简单、使用方便、成本较低廉。
有益效果
抗生素是治疗细菌感染的有效药物,细菌耐药性的产生是细菌天然抵抗抗生素的一种现象,只要使用抗生素,细菌就不可避免的对这种抗生素产生耐药性,是自然界的普遍规律。现今大量耐药菌层出不穷,使抗生素的疗效受到严重威胁,自从抗生素开始使用,就有大量耐药菌报道,对人类来说这是一个很大的挑战。细菌会在短时间内快速对抗生素产生耐药性。在青霉素使用一年之后即有耐青霉素的金黄色葡萄球菌产生,在1959年有报道甲氧西林可治疗由耐药金黄色葡萄球菌引起的感染,然而在两年之后就有耐甲氧西林金黄色葡萄球菌产生,由此可见耐药性产生之迅速。现今很多一代抗生素已经无效,且细菌对抗生素的耐药率持续升高。在我国现细菌耐药性仍呈增长趋势,多重耐药和广泛耐药菌株检出率的增加对临床抗感染治疗构成严重威胁。耐药菌传播迅速,耐药基因不仅可种内传播,甚至可种间传播,动物源耐药菌的耐药基因也可传播到人源病原菌,严重威胁人类的健康。
由于不利环境的持续和为满足高速生长要求所必须饲料的高能量高蛋白组成的刺激,寒湿型泄泻已经成为养殖动物的主要临床危害病症之一。广泛普遍采用抗生素予以防治,是目前养殖业最常见的措施,然而,其效果却难于令人满意。虽然目前国家专利局已经受理或审批多项抗猪禽腹泻的中药专利,但这些专利,大部分为组方是根据西医思想组方,不能对证,且大多为散剂,使用也不方便;有点虽然为煎液,但普遍组方复杂,剂型粗放,沉淀物多而易造成养殖舍饮水管道堵塞,而且成分复杂,产品无质量监控或监控困难;还有的则仅为预防用。
本发明首次将石榴皮、广藿香、吴茱萸等三味中药对病证进行客观辨证后按照中药配伍的原则组成复方合剂,用于治疗猪寒湿型泄泻。结果发现,用药2次仔猪泄泻即痊愈,且连续观察一周未见复发;庆大霉素对照组用药4天后痊愈,杨树花口服液(三类新中兽药,专用于猪腹泻)组用药3次后痊愈,但在随后的一周内,均陆续见30.77%~39.53%复发。本复方组方和制剂工艺简单,使用方便,成本低廉,可用于猪寒湿型泄泻的临床防治。
与现有技术相比,本发明优点如下:
1.可用于猪寒湿型泄泻的临床治疗,且疗效确实,不易复发。本发明提供的中药复方对猪寒湿型泄泻的疗程比对照药组缩短1~2天,复发率降低30~45%。
2.组方和制剂工艺简单,使用方便,质量监控简单。目前专用于寒湿型泄泻症的治疗用药尚缺乏。目前公布的防治猪腹泻的专利大部分为散剂,使用不方便;有的虽为煎液,但组方复杂,质量控制困难;还有的则仅为腹泻预防用。杨树花口服液虽然用于猪腹泻,但也是对腹泻的广泛性药物,不对证,易复发。而本复方组方和制剂工艺简单,质量监控简单,使用方便,成本也较低廉。
四、具体实施方式
1.中药复方的制备
各取中药石榴皮、广藿香、吴茱萸分别为主、辅、佐使药。按照石榴皮1000g、广藿香30g、吴茱萸20g;取广藿香、吴茱萸加6倍质量的石油醚,浸泡12h,调整超声仪温度40℃、功率220W、时间40min,超声震荡萃取,反复2次,合并萃取液,用旋转蒸发仪真空浓缩,挥发净溶剂后加入50ml无水酒精待用;广藿香、吴茱萸萃取后药渣挥发净溶剂后与石榴皮合并,加入15倍蒸馏水,浸泡40min后,武火煎煮迅速至沸腾,改为文火维持沸腾60分钟,收集的煎煮液;药渣再加15倍质量的水,同上法煎煮,收集并合并2次煎煮液,4层纱布过滤煎煮液,并隔水浓缩至950mL,加入酒精溶解的挥发油、苯甲酸钠15克,充分摇匀,分装入鸭嘴瓶,备用。
2.中药提取物对寒湿型泄泻大鼠模型的治疗作用
清洁级Wistar大鼠,除空白对照组外,其余大鼠每天上下午分别灌胃生大黄热浸提液(1mL/100g)和猪油(1mL/100g),空白组灌服(1mL/100g)常温生理盐水,每日上下午各一次,持续15d。从第16天开始,将灌药大鼠分为模型组、高剂量和低剂量组;高剂量组和低剂量组分别按照1mL/kg和0.25mL/kg灌服中药复方合剂,模型组和空白对照组灌服等体积生理盐水,每天1次,连续3天。分别观察大鼠腹泻指数和粪便含水量、肠道木糖吸收和肠管推进率。
如表1所示,模型组的腹泻指数和粪便含水量与空白组相比显著升高;用药后,高剂量组的腹泻指数和粪便含水量较模型组显著降低,说明高剂量组对腹泻有明显治疗效果。
表1各组大鼠腹泻指数和粪便含水量测定结果
*表示该组与空白组相比差异显著(P<0.05),#表示该组与模型组相比差异显著(P<0.05)
如表2所示,模型组的肠道木糖浓度和肠管推进与空白对照组相比分别极显著降低和升高;高剂量组较模型组的异常改变有极显著的改善。
表2各组大鼠肠管吸收和肠管推进率
**表示该组与空白组相比差异极显著(P<0.01)。##表示该组与模型组相比差异极显著(P<0.01)。
3.中药提取物对自然发病寒湿型泄泻仔猪的治疗作用
10-22日龄泄泻仔猪(粪便稀薄、色黄白,有的似糊状,有的水样,仔猪普遍杂毛,寒颤,大部分仔猪肛周及后躯见大量粪便污染,经辨证为寒湿泄泻)294头,按栏位随机分为4组:庆大霉素组、杨树花口服液组、中药复方组和庆大霉素+杨树花口服液组。庆大霉素组各只仔猪灌服4万单位庆大霉素注射液,杨树花口服液组每只仔猪灌服2ml,中药复方组灌服2ml,庆大霉素+杨树花口服液组灌服4万单位庆大霉素+2ml杨树花口服液,每天1次,视病情情况连续用药3~4天,并持续观察1周。每天观察记录和统计各组仔猪腹泻情况,并统计腹泻指数。腹泻指数评分标准见表3:
表3仔猪腹泻指数评分标准
各组仔猪治愈率及复发情况见表4。到第5天,各组仔猪均治愈。到第3天,中药复方组即全部治愈,极显著优于其他各组,庆大霉素组到第4天治愈率仅93.80%,杨树花口服液与庆大霉素效果基本接近。连续观察一周,中药复方组未见复发,而其他各组均有30%以上复发率。
表4各组泄泻仔猪治愈率(%)
同列肩标小写字母不同表示差异显著(P<0.05),大写字母不同表示差异极显著(P<0.01),字母相同表示差异不显著(P>0.05)。
各组仔猪腹泻指数评分情况见表5。治疗第1天,各组腹泻指数没有差异,第2天,庆大霉素组腹泻指数显著高于其他各组,中药复方组略低于其他两个用药组。到第3天,中药复方组显著低于其他3组,未再见腹泻发生;其他3组间未见差异。
表5各组仔猪腹泻指数评分结果
同列肩标小写字母不同表示差异显著(P<0.05),大写字母不同表示差异极显著(P<0.01),字母相同表示差异不显著(P>0.05)。
Claims (2)
1.一种治疗猪寒湿型泄泻的中药复方合剂,其特征在于,原料由石榴皮1000g、广藿香30g、吴茱萸20g组成;其制备方法为按组方量取广藿香、吴茱萸,加6倍质量的石油醚,浸泡12h,调整超声仪温度为40℃、功率220W、时间40min,超声震荡萃取,反复2次,合并萃取液,用旋转蒸发仪真空浓缩,所得挥发油挥发净溶剂后加入50ml无水乙醇溶解待用;广藿香、吴茱萸萃取后药渣挥发净溶剂后与石榴皮合并,加入15倍蒸馏水,浸泡40min后,武火煎煮迅速至沸腾,改为文火维持沸腾60分钟,收集煎液;同法重复煎煮1次,收集并合并2次煎液后,用4层纱布过滤,滤液隔水浓缩至950mL,加入无水乙醇溶解的挥发油、苯甲酸钠15g,充分摇匀,分装入鸭嘴瓶,备用。
2.权利要求1所述的中药复方合剂在制备猪寒湿型泄泻证防治药物中的应用。
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