CN108095187A - A kind of enrichment method of tobacco glucosides - Google Patents
A kind of enrichment method of tobacco glucosides Download PDFInfo
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- CN108095187A CN108095187A CN201711194257.5A CN201711194257A CN108095187A CN 108095187 A CN108095187 A CN 108095187A CN 201711194257 A CN201711194257 A CN 201711194257A CN 108095187 A CN108095187 A CN 108095187A
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- A—HUMAN NECESSITIES
- A24—TOBACCO; CIGARS; CIGARETTES; SIMULATED SMOKING DEVICES; SMOKERS' REQUISITES
- A24B—MANUFACTURE OR PREPARATION OF TOBACCO FOR SMOKING OR CHEWING; TOBACCO; SNUFF
- A24B15/00—Chemical features or treatment of tobacco; Tobacco substitutes, e.g. in liquid form
- A24B15/18—Treatment of tobacco products or tobacco substitutes
- A24B15/24—Treatment of tobacco products or tobacco substitutes by extraction; Tobacco extracts
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Abstract
The present invention relates to a kind of enrichment methods of tobacco glucosides, belong to technical field of cigarette production.The present invention, using 100nm, 10nm, the united application of 3 5nm apertures films, removes the non-glucosides material collection tobacco glucosides in tobacco according to the physicochemical property difference of tobacco different chemical composition.The water extract of tobacco material is after centrifugation or chemical settling removal of impurities, the true solution of tobacco extract is prepared into using solid contents such as 100nm apertures film removing silt, smashed tobacco powder, fibers, the true solution is handled through 10nm apertures membrane filtration, remove the soluble high-molecular substance in solution, for the permeate of 10nm apertures film again through 3 5nm apertures membrane filtration processing except impurity such as desaccharification, salt, the trapped fluid of 3 5nm apertures films is the solution for being enriched tobacco glucosides.The advantages that technical method need not use organic solvent, environmental-friendly, economical good, tobacco glucosides enrichment high income, applicable large-scale production.
Description
Technical field
The invention belongs to technical field of cigarette production, and in particular to a kind of physicochemical property according to tobacco different chemical composition
Difference handles tobacco extract, to be enriched with the process of tobacco glucosides using membrane separation technique.
Background technology
Glucosides is also known as glycocide, is the officials such as hemiacetal hydroxyl and hydroxyl, the sulfydryl in compound molecule of monose or oligosaccharides
The compound that dehydration can be rolled into a ball and formed.Glucosides in tobacco is the important latent Studies of The Aromatic Substances of tobacco, fragrance characteristic and suction to cigarette
Food matter plays an important role.
Tobacco glucosides can be obtained by separate tobacco chemical composition or synthetic method.The separation chemistry ingredient from tobacco
Usual way is first water extraction tobacco when preparing glucosides, then with the water-soluble liquid enrichment glucosides of extracting n-butyl alcohol, is purified through column chromatography
And it obtains.When preparing glucosides using synthetic method, generally all prepared using the bromide of aglycon and sugar by substitution reaction.It is previous
Method is of high cost, is not suitable for the production of scale；Although latter method can obtain the very high some tobacco glycoside matters of purity,
But it is difficult to obtain consistent with tobacco glucosides composition or approximate glucosides mixture, cigarette application is restricted, and manufacturing cost is also high.
At present, there is not yet being enriched with the article and patent report of glucosides from tobacco with membrane separation technique.
The content of the invention
It is an object of the present invention to solve the deficiency of the existing technology and provide a kind of enrichment method of tobacco glucosides, the skills
Art method need not use organic solvent, environmental-friendly, economical good, and tobacco glucosides enrichment high income is applicable in scale
The advantages that production.
To achieve the above object, the technical solution adopted by the present invention is as follows：
A kind of enrichment method of tobacco glucosides, includes the following steps：
Step（1）, the preparation of tobacco suspension turbid：By tobacco material aqueous extract of the dry matter content not higher than 25% through centrifugation
Or after chemical settling, precipitation is abandoned, obtains tobacco suspension turbid；
The tobacco material is at least one of offal, offal and tobacco leaf；
The chemical settling is 0.2% chitosan as sedimentation agent using mass concentration；
Step（2）, once filter：By step（1）Obtained tobacco suspension turbid through 100nm apertures membrane filtration, when trapped fluid and
Permeate volume ratio is 1：When 19, the water that volume is tobacco suspension turbid volume 1/10 is added in into trapped fluid, is continued after mixing
100nm apertures film is crossed, until the volume of final trapped fluid is the 5% of tobacco suspension turbid volume, filtering terminates；
Step（3）, secondary filter：By step（2）Obtained permeate is through 10nm apertures membrane filtration, when the volume of trapped fluid is cigarette
The 5% of careless suspension turbid liquid volume adds in the water that volume is tobacco suspension turbid volume 1/10 into trapped fluid, continued after mixing
10nm pore size ceramic membranes, until the volume of final trapped fluid is the 5% of tobacco suspension turbid volume, filtering terminates；
Step（4）, filter three times：By step（3）Obtained permeate is through 3-5nm apertures membrane filtration, when the volume of trapped fluid is
Tobacco suspension turbid volume 5% when, the water that volume is tobacco suspension turbid volume 1/10 is added in into trapped fluid, mixing is follow-up
Continued 3-5nm apertures film to trapped fluid volume for tobacco suspension turbid volume 5% and then once add in into trapped fluid
Volume is the water of tobacco suspension turbid volume 1/10, and the volume for continuing 3-5nm apertures film to final trapped fluid after mixing is cigarette
The 5% of careless suspension turbid liquid volume, obtained final trapped fluid are the solution for being enriched tobacco glucosides, and the trapped fluid is by steaming
After hair concentration, sticky tobacco glucosides is obtained.
It is further preferred that step（1）In, the tobacco material aqueous extract dry matter content is 6-15%.
It is further preferred that step（1）In, tobacco material aqueous extract in preparation process, tobacco material and water
Weight ratio is less than 1:3.
It is further preferred that the weight ratio of tobacco material and water is 1:5-1:7.
It is further preferred that step（2）In, 100nm apertures film is organic film or inoranic membrane, and operating pressure is less than
0.5MPa；When for inoranic membrane when, operating temperature range be 0-100 DEG C；When for organic film when, operating temperature range be 0-70 DEG C.
It is further preferred that step（3）In, the operating pressure of 10nm apertures film is 0.2-0.8MPa.
It is further preferred that step（4）In, 3-5nm apertures film be organic film or inoranic membrane, operating pressure 0.4-
1.5MPa；When for inoranic membrane when, operating temperature range be 0-100 DEG C；When for organic film when, operating temperature range be 0-70 DEG C.
As alternate embodiments, the chemical settling can be using diatomite as sedimentation agent.
The present invention has following technical characterstic
（1）The enrichment of tobacco glucosides is the film in suitable aperture is selected to remove other materials, the making material with film, the fortune of film
Line mode is unrelated.
（2）Tobacco glycosidic nature is relatively stable, therefore temperature can be run in the case where film is resistant to.
（3）The operating pressure of film operation is all suitable in the range of equipment design.
（4）The operation concentration of tobacco extract（Refer to extracting solution dry matter content）Do not exceed 25%, suitable concentration 6-15%.
Obtained in the present invention after centrifugation or chemical settling in tobacco suspension turbid also containing a small amount of silt, smashed tobacco powder,
The solid contents such as fiber；
The purpose that once filters of the present invention is that retention removes the silt of tobacco suspension turbid remaining, smashed tobacco powder, fiber and again
The substances such as protein, the pectin of segregation.
The purpose of secondary filter of the present invention is that retention removes soluble protein and soluble pectin etc.（It can be referred to as
Soluble polymer substance A）.
The purpose that the present invention filters three times is except desaccharification, acylate and inorganic acid salt, and permeate is containing sugar, acylate
With the solution of inorganic acid salt, which is the solution rich in tobacco glucosides（It can be referred to as component B tobacco glucosides）.
On film：The film that the present invention uses is unrelated with the material for making film, only related in aperture（It is limited when secondary filter
Fixed is ceramic membrane, and inventor is asked to verify again）；Temperature is related to membrane material during membrane filtration, and inoranic membrane can be organic at 0-100 DEG C
Film can be at 0-70 DEG C（Most of organic films are at 0-50 DEG C）, operating pressure：It is related to membrane aperture, during 100nm, it is generally less than
0.3MPa；During 10nm, 0.2-0.5MPa is typically in the range of,；During 3-5nm, 0.2-1MPa is typically in the range of, during membrane filtration, fouling membrane is asked
Topic may cause flow velocity unstable, so generally not saying flow velocity problem.
Preparation on tobacco extract：Water is solvent, and water is more than 3 with material quantity by weight if:1 can carry out
Extraction, more suitable ratio is 5:1-7:1, it is advisable twice if intermittent, continous way does not have fixed water and material quantity
Than normal pressure, decompression, pressurization all may be used.For temperature at 0-120 DEG C, extraction is not emphasis, and the richness of glucosides is can be used for as long as aqueous solution
Collection.
Compared with prior art, the present invention its advantage is：
The present invention utilizes the molecular particle size difference of different chemical composition in tobacco, adopts the combination of 100nm, 10nm, 3-5nm aperture film
The glycoside matter being enriched in tobacco, belongs to physical separation method.With the n-butanol using tobacco different chemical composition polarity difference
Extracting process compares, which avoids the use of organic solvent, and the Glycosides Contents of enrichment are up to more than 85%, hence it is evident that n-butanol
50% or so of extracting process, and the separation yield of glycoside matter is up to more than 95%, far above apparent extracting n-butyl alcohol method 80%
Left and right has significant advantage in addition in the method cost, environmentally friendly, is suitble to the production of scale.
Description of the drawings
Fig. 1 is the process flow chart of the enrichment method of tobacco glucosides of the present invention.
Specific embodiment
With reference to embodiment, the present invention is described in further detail.
It will be understood to those of skill in the art that the following example is merely to illustrate the present invention, and it should not be regarded as limiting this hair
Bright scope.In the examples where no specific technique or condition is specified, according to the described technology of document in the art or condition
Or it is carried out according to product description.Reagents or instruments used without specified manufacturer, being can be by buying what is obtained
Conventional products.
The present invention is unless otherwise stated, percentage sign is mass percent.
Embodiment 1
A kind of enrichment method of tobacco glucosides, includes the following steps：
Step（1）, the preparation of tobacco suspension turbid：By dry matter content be 15% tobacco material aqueous extract after centrifugation, abandon
Precipitation, obtains tobacco suspension turbid；
The tobacco material is offal；
The chemical settling is 0.2% chitosan as sedimentation agent using mass concentration；
Step（2）, once filter：By step（1）Obtained tobacco suspension turbid through 100nm apertures membrane filtration, when trapped fluid and
Permeate volume ratio is 1：When 19, the water that volume is tobacco suspension turbid volume 1/10 is added in into trapped fluid, is continued after mixing
100nm apertures film is crossed, until the volume of final trapped fluid is the 5% of tobacco suspension turbid volume, filtering terminates；
Step（3）, secondary filter：By step（2）Obtained permeate is through 10nm apertures membrane filtration, when the volume of trapped fluid is cigarette
The 5% of careless suspension turbid liquid volume adds in the water that volume is tobacco suspension turbid volume 1/10 into trapped fluid, continued after mixing
10nm pore size ceramic membranes, until the volume of final trapped fluid is the 5% of tobacco suspension turbid volume, filtering terminates；
Step（4）, filter three times：By step（3）Obtained permeate is through 3-5nm apertures membrane filtration, when the volume of trapped fluid is
Tobacco suspension turbid volume 5% when, the water that volume is tobacco suspension turbid volume 1/10 is added in into trapped fluid, mixing is follow-up
Continued 3-5nm apertures film to trapped fluid volume for tobacco suspension turbid volume 5% and then once add in into trapped fluid
Volume is the water of tobacco suspension turbid volume 1/10, and the volume for continuing 3-5nm apertures film to final trapped fluid after mixing is cigarette
The 5% of careless suspension turbid liquid volume, obtained final trapped fluid are the solution for being enriched tobacco glucosides, and the trapped fluid is by steaming
After hair concentration, sticky tobacco glucosides is obtained.
Wherein, step（1）In, for tobacco material aqueous extract in preparation process, the weight ratio of tobacco material and water is 1:
4。
Step（2）In, 100nm apertures film is organic film, and operating pressure is less than 0.5MPa, operating temperature range 0-70
℃。
Step（3）In, the operating pressure of 10nm apertures film is 0.2-0.8MPa.
Step（4）In, 3-5nm apertures film be inoranic membrane, operating pressure 0.4-1.5MPa, operating temperature range 0-
100℃。
Embodiment 2
A kind of enrichment method of tobacco glucosides, includes the following steps：
Step（1）, the preparation of tobacco suspension turbid：By dry matter content be 6% tobacco material aqueous extract after centrifugation, abandon
Precipitation, obtains tobacco suspension turbid；
The tobacco material is offal；
The chemical settling is 0.2% chitosan as sedimentation agent using mass concentration；
Step（2）, once filter：By step（1）Obtained tobacco suspension turbid through 100nm apertures membrane filtration, when trapped fluid and
Permeate volume ratio is 1：When 19, the water that volume is tobacco suspension turbid volume 1/10 is added in into trapped fluid, is continued after mixing
100nm apertures film is crossed, until the volume of final trapped fluid is the 5% of tobacco suspension turbid volume, filtering terminates；
Step（3）, secondary filter：By step（2）Obtained permeate is through 10nm apertures membrane filtration, when the volume of trapped fluid is cigarette
The 5% of careless suspension turbid liquid volume adds in the water that volume is tobacco suspension turbid volume 1/10 into trapped fluid, continued after mixing
10nm pore size ceramic membranes, until the volume of final trapped fluid is the 5% of tobacco suspension turbid volume, filtering terminates；
Step（4）, filter three times：By step（3）Obtained permeate is through 3-5nm apertures membrane filtration, when the volume of trapped fluid is
Tobacco suspension turbid volume 5% when, the water that volume is tobacco suspension turbid volume 1/10 is added in into trapped fluid, mixing is follow-up
Continued 3-5nm apertures film to trapped fluid volume for tobacco suspension turbid volume 5% and then once add in into trapped fluid
Volume is the water of tobacco suspension turbid volume 1/10, and the volume for continuing 3-5nm apertures film to final trapped fluid after mixing is cigarette
The 5% of careless suspension turbid liquid volume, obtained final trapped fluid are the solution for being enriched tobacco glucosides, and the trapped fluid is by steaming
After hair concentration, sticky tobacco glucosides is obtained.
Wherein, step（1）In, for tobacco material aqueous extract in preparation process, the weight ratio of tobacco material and water is 1:
5。
Step（2）In, 100nm apertures film is inoranic membrane, and operating pressure is less than 0.5MPa, operating temperature range 0-100
℃。
Step（3）In, the operating pressure of 10nm apertures film is 0.2-0.8MPa.
Step（4）In, 3-5nm apertures film is that organic film operating pressure is 0.4-1.5MPa, operating temperature range 0-70
℃。
Embodiment 3
A kind of enrichment method of tobacco glucosides, includes the following steps：
Step（1）, the preparation of tobacco suspension turbid：By dry matter content be 25% tobacco material aqueous extract through chemical settling
Afterwards, precipitation is abandoned, obtains tobacco suspension turbid；
The tobacco material is tobacco leaf；
The chemical settling is using diatomite as sedimentation agent；
Step（2）, once filter：By step（1）Obtained tobacco suspension turbid through 100nm apertures membrane filtration, when trapped fluid and
Permeate volume ratio is 1：When 19, the water that volume is tobacco suspension turbid volume 1/10 is added in into trapped fluid, is continued after mixing
100nm apertures film is crossed, until the volume of final trapped fluid is the 5% of tobacco suspension turbid volume, filtering terminates；
Step（3）, secondary filter：By step（2）Obtained permeate is through 10nm apertures membrane filtration, when the volume of trapped fluid is cigarette
The 5% of careless suspension turbid liquid volume adds in the water that volume is tobacco suspension turbid volume 1/10 into trapped fluid, continued after mixing
10nm pore size ceramic membranes, until the volume of final trapped fluid is the 5% of tobacco suspension turbid volume, filtering terminates；
Step（4）, filter three times：By step（3）Obtained permeate is through 3-5nm apertures membrane filtration, when the volume of trapped fluid is
Tobacco suspension turbid volume 5% when, the water that volume is tobacco suspension turbid volume 1/10 is added in into trapped fluid, mixing is follow-up
Continued 3-5nm apertures film to trapped fluid volume for tobacco suspension turbid volume 5% and then once add in into trapped fluid
Volume is the water of tobacco suspension turbid volume 1/10, and the volume for continuing 3-5nm apertures film to final trapped fluid after mixing is cigarette
The 5% of careless suspension turbid liquid volume, obtained final trapped fluid are the solution for being enriched tobacco glucosides, and the trapped fluid is by steaming
After hair concentration, sticky tobacco glucosides is obtained.
Wherein, step（1）In, for tobacco material aqueous extract in preparation process, the weight ratio of tobacco material and water is 1:
7。
Step（2）In, 100nm apertures film is inoranic membrane, and operating pressure is less than 0.5MPa, operating temperature range 0-100
℃。
Step（3）In, the operating pressure of 10nm apertures film is 0.2-0.8MPa.
Step（4）In, 3-5nm apertures film be inoranic membrane, operating pressure 0.4-1.5MPa, operating temperature range 0-
100℃。
Embodiment 4
A kind of enrichment method of tobacco glucosides, includes the following steps：
Step（1）, the preparation of tobacco suspension turbid：By dry matter content be 20% tobacco material aqueous extract through chemical settling
Afterwards, precipitation is abandoned, obtains tobacco suspension turbid；
The tobacco material is offal and offal；（Mass ratio is 1：1）
The chemical settling is 0.2% chitosan as sedimentation agent using mass concentration；
Step（2）, once filter：By step（1）Obtained tobacco suspension turbid through 100nm apertures membrane filtration, when trapped fluid and
Permeate volume ratio is 1：When 19, the water that volume is tobacco suspension turbid volume 1/10 is added in into trapped fluid, is continued after mixing
100nm apertures film is crossed, until the volume of final trapped fluid is the 5% of tobacco suspension turbid volume, filtering terminates；
Step（3）, secondary filter：By step（2）Obtained permeate is through 10nm apertures membrane filtration, when the volume of trapped fluid is cigarette
The 5% of careless suspension turbid liquid volume adds in the water that volume is tobacco suspension turbid volume 1/10 into trapped fluid, continued after mixing
10nm pore size ceramic membranes, until the volume of final trapped fluid is the 5% of tobacco suspension turbid volume, filtering terminates；
Step（4）, filter three times：By step（3）Obtained permeate is through 3-5nm apertures membrane filtration, when the volume of trapped fluid is
Tobacco suspension turbid volume 5% when, the water that volume is tobacco suspension turbid volume 1/10 is added in into trapped fluid, mixing is follow-up
Continued 3-5nm apertures film to trapped fluid volume for tobacco suspension turbid volume 5% and then once add in into trapped fluid
Volume is the water of tobacco suspension turbid volume 1/10, and the volume for continuing 3-5nm apertures film to final trapped fluid after mixing is cigarette
The 5% of careless suspension turbid liquid volume, obtained final trapped fluid are the solution for being enriched tobacco glucosides, and the trapped fluid is by steaming
After hair concentration, sticky tobacco glucosides is obtained.
Wherein, step（1）In, for tobacco material aqueous extract in preparation process, the weight ratio of tobacco material and water is 1:
6。
Step（2）In, 100nm apertures film is organic film or inoranic membrane, and operating pressure is less than 0.5MPa；When for inoranic membrane
When, operating temperature range is 0-100 DEG C；When for organic film when, operating temperature range be 0-70 DEG C.
Step（3）In, the operating pressure of 10nm apertures film is 0.2-0.8MPa.
Step（4）In, 3-5nm apertures film is that organic film operating pressure is 0.4-1.5MPa, operating temperature range 0-70
℃。
Embodiment 5
A kind of enrichment method of tobacco glucosides, includes the following steps：
Step（1）, the preparation of tobacco suspension turbid：By dry matter content be 10% tobacco material aqueous extract through chemical settling
Afterwards, precipitation is abandoned, obtains tobacco suspension turbid；
The tobacco material is offal, offal and tobacco leaf；（Mass ratio is 1：1：2）
The chemical settling is 0.2% chitosan as sedimentation agent using mass concentration；
Step（2）, once filter：By step（1）Obtained tobacco suspension turbid through 100nm apertures membrane filtration, when trapped fluid and
Permeate volume ratio is 1：When 19, the water that volume is tobacco suspension turbid volume 1/10 is added in into trapped fluid, is continued after mixing
100nm apertures film is crossed, until the volume of final trapped fluid is the 5% of tobacco suspension turbid volume, filtering terminates；
Step（3）, secondary filter：By step（2）Obtained permeate is through 10nm apertures membrane filtration, when the volume of trapped fluid is cigarette
The 5% of careless suspension turbid liquid volume adds in the water that volume is tobacco suspension turbid volume 1/10 into trapped fluid, continued after mixing
10nm pore size ceramic membranes, until the volume of final trapped fluid is the 5% of tobacco suspension turbid volume, filtering terminates；
Step（4）, filter three times：By step（3）Obtained permeate is through 3-5nm apertures membrane filtration, when the volume of trapped fluid is
Tobacco suspension turbid volume 5% when, the water that volume is tobacco suspension turbid volume 1/10 is added in into trapped fluid, mixing is follow-up
Continued 3-5nm apertures film to trapped fluid volume for tobacco suspension turbid volume 5% and then once add in into trapped fluid
Volume is the water of tobacco suspension turbid volume 1/10, and the volume for continuing 3-5nm apertures film to final trapped fluid after mixing is cigarette
The 5% of careless suspension turbid liquid volume, obtained final trapped fluid are the solution for being enriched tobacco glucosides, and the trapped fluid is by steaming
After hair concentration, sticky tobacco glucosides is obtained.
Wherein, step（1）In, for tobacco material aqueous extract in preparation process, the weight ratio of tobacco material and water is 1:
8。
Step（2）In, 100nm apertures film is organic film, and operating pressure is less than 0.5MPa, operating temperature range 0-70
℃。
Step（3）In, the operating pressure of 10nm apertures film is 0.2-0.8MPa.
Step（4）In, 3-5nm apertures film is that organic film operating pressure is 0.4-1.5MPa, operating temperature range 0-70
℃。
Comparative example 1
Comparative example 1 and embodiment 5 difference lies in：By step（2）In when trapped fluid and permeate volume ratio be 1：When 19, modification
It is 1 to work as trapped fluid and permeate volume ratio：When 15
Comparative example 2
Comparative example 2 and embodiment 5 difference lies in：The dry matter content of tobacco material aqueous extract is 35%.
Comparative example 3
Comparative example 3 and embodiment 5 difference lies in：Step（4）It is revised as：It filters three times：By step（3）Obtained permeate warp
3-5nm apertures membrane filtration, when the volume of trapped fluid is the 5% of tobacco suspension turbid volume, it is cigarette that volume is added in into trapped fluid
The water of careless suspension turbid liquid volume 1/10, the volume for continuing 3-5nm apertures film to trapped fluid after mixing are tobacco suspension turbid body
Long-pending 5%, obtained trapped fluid are the solution for being enriched tobacco glucosides, which obtains tobacco after evaporation and concentration
Glucosides.
Technique effect is evaluated
The embodiment of the present invention, comparative example and n-butanol extraction are compared, the results are shown in Table shown in 1.
Table 1
In terms of the enrichment yield and purity of table 1, the present invention has significant advantage than traditional n-butanol extraction.And this hair
Bright embodiment with comparative example excellent effect, illustrates that step has good synergistic effect to the present invention up and down.
Basic principle, main feature and the advantages of the present invention of the present invention has been shown and described above.The technology of the industry
Personnel are it should be appreciated that the present invention is not limited to the above embodiments, and the above embodiments and description only describe this
The principle of invention, without departing from the spirit and scope of the present invention, various changes and modifications of the present invention are possible, these changes
Change and improvement all fall within the protetion scope of the claimed invention.The claimed scope of the invention by appended claims and its
Equivalent thereof.
Claims (8)
1. a kind of enrichment method of tobacco glucosides, which is characterized in that include the following steps：
Step（1）, the preparation of tobacco suspension turbid：By tobacco material aqueous extract of the dry matter content not higher than 25% through centrifugation
Or after chemical settling, precipitation is abandoned, obtains tobacco suspension turbid；
The tobacco material is at least one of offal, offal and tobacco leaf；
The chemical settling is 0.2% chitosan as sedimentation agent using mass concentration；
Step（2）, once filter：By step（1）Obtained tobacco suspension turbid through 100nm apertures membrane filtration, when trapped fluid and
Permeate volume ratio is 1：When 19, the water that volume is tobacco suspension turbid volume 1/10 is added in into trapped fluid, is continued after mixing
100nm apertures film is crossed, until the volume of final trapped fluid is the 5% of tobacco suspension turbid volume, filtering terminates；
Step（3）, secondary filter：By step（2）Obtained permeate is through 10nm apertures membrane filtration, when the volume of trapped fluid is cigarette
The 5% of careless suspension turbid liquid volume adds in the water that volume is tobacco suspension turbid volume 1/10 into trapped fluid, continued after mixing
10nm pore size ceramic membranes, until the volume of final trapped fluid is the 5% of tobacco suspension turbid volume, filtering terminates；
Step（4）, filter three times：By step（3）Obtained permeate is through 3-5nm apertures membrane filtration, when the volume of trapped fluid is
Tobacco suspension turbid volume 5% when, the water that volume is tobacco suspension turbid volume 1/10 is added in into trapped fluid, mixing is follow-up
Continued 3-5nm apertures film to trapped fluid volume for tobacco suspension turbid volume 5% and then once add in into trapped fluid
Volume is the water of tobacco suspension turbid volume 1/10, and the volume for continuing 3-5nm apertures film to final trapped fluid after mixing is cigarette
The 5% of careless suspension turbid liquid volume, obtained final trapped fluid are the solution for being enriched tobacco glucosides, and the trapped fluid is by steaming
After hair concentration, tobacco glucosides is obtained.
2. the enrichment method of tobacco glucosides according to claim 1, which is characterized in that step（1）In, the tobacco is former
It is 6-15% to expect aqueous extract dry matter content.
3. the enrichment method of tobacco glucosides according to claim 1, which is characterized in that step（1）In, tobacco material water carries
Liquid is taken in preparation process, the weight ratio of tobacco material and water is less than 1:3.
4. the enrichment method of tobacco glucosides according to claim 3, which is characterized in that the weight ratio of tobacco material and water is
1:5-1:7。
5. the enrichment method of tobacco glucosides according to claim 1, which is characterized in that step（2）In, 100nm apertures film
For organic film or inoranic membrane, operating pressure is less than 0.5MPa；When for inoranic membrane when, operating temperature range be 0-100 DEG C；When to have
During machine film, operating temperature range is 0-70 DEG C.
6. the enrichment method of tobacco glucosides according to claim 1, which is characterized in that step（3）In, 10nm apertures film
Operating pressure is 0.2-0.8MPa.
7. the enrichment method of tobacco glucosides according to claim 1, which is characterized in that step（4）In, 3-5nm apertures film
For organic film or inoranic membrane, operating pressure 0.4-1.5MPa；When for inoranic membrane when, operating temperature range be 0-100 DEG C；When for
During organic film, operating temperature range is 0-70 DEG C.
8. the enrichment method of tobacco glucosides according to claim 1, which is characterized in that the chemical settling can be with silicon
Diatomaceous earth is sedimentation agent.
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| CN201711194257.5A CN108095187B (en) | 2017-11-24 | 2017-11-24 | Method for enriching tobacco glucoside |
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