CN108094269A - A kind of fish meal for improving breeding efficiency and preparation method thereof, feeding method - Google Patents
A kind of fish meal for improving breeding efficiency and preparation method thereof, feeding method Download PDFInfo
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- CN108094269A CN108094269A CN201711412272.2A CN201711412272A CN108094269A CN 108094269 A CN108094269 A CN 108094269A CN 201711412272 A CN201711412272 A CN 201711412272A CN 108094269 A CN108094269 A CN 108094269A
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- 235000019733 Fish meal Nutrition 0.000 title claims abstract description 39
- 239000004467 fishmeal Substances 0.000 title claims abstract description 39
- 238000009395 breeding Methods 0.000 title claims abstract description 20
- 230000001488 breeding effect Effects 0.000 title claims abstract description 20
- 238000000034 method Methods 0.000 title claims abstract description 18
- 238000002360 preparation method Methods 0.000 title claims abstract description 13
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 38
- 229920002752 Konjac Polymers 0.000 claims abstract description 34
- 229920002581 Glucomannan Polymers 0.000 claims abstract description 30
- LUEWUZLMQUOBSB-FSKGGBMCSA-N (2s,3s,4s,5s,6r)-2-[(2r,3s,4r,5r,6s)-6-[(2r,3s,4r,5s,6s)-4,5-dihydroxy-2-(hydroxymethyl)-6-[(2r,4r,5s,6r)-4,5,6-trihydroxy-2-(hydroxymethyl)oxan-3-yl]oxyoxan-3-yl]oxy-4,5-dihydroxy-2-(hydroxymethyl)oxan-3-yl]oxy-6-(hydroxymethyl)oxane-3,4,5-triol Chemical compound O[C@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1O[C@@H]1[C@@H](CO)O[C@@H](O[C@@H]2[C@H](O[C@@H](OC3[C@H](O[C@@H](O)[C@@H](O)[C@H]3O)CO)[C@@H](O)[C@H]2O)CO)[C@H](O)[C@H]1O LUEWUZLMQUOBSB-FSKGGBMCSA-N 0.000 claims abstract description 29
- 229940046240 glucomannan Drugs 0.000 claims abstract description 29
- 235000010485 konjac Nutrition 0.000 claims abstract description 29
- 244000046052 Phaseolus vulgaris Species 0.000 claims abstract description 11
- 235000010627 Phaseolus vulgaris Nutrition 0.000 claims abstract description 11
- 229920002472 Starch Polymers 0.000 claims abstract description 11
- YYRMJZQKEFZXMX-UHFFFAOYSA-L calcium bis(dihydrogenphosphate) Chemical compound [Ca+2].OP(O)([O-])=O.OP(O)([O-])=O YYRMJZQKEFZXMX-UHFFFAOYSA-L 0.000 claims abstract description 11
- 229940062672 calcium dihydrogen phosphate Drugs 0.000 claims abstract description 11
- 229910000389 calcium phosphate Inorganic materials 0.000 claims abstract description 11
- OEYIOHPDSNJKLS-UHFFFAOYSA-N choline Chemical compound C[N+](C)(C)CCO OEYIOHPDSNJKLS-UHFFFAOYSA-N 0.000 claims abstract description 11
- 229960001231 choline Drugs 0.000 claims abstract description 11
- 239000011707 mineral Substances 0.000 claims abstract description 11
- 235000019691 monocalcium phosphate Nutrition 0.000 claims abstract description 11
- 239000010499 rapseed oil Substances 0.000 claims abstract description 11
- 239000008107 starch Substances 0.000 claims abstract description 11
- 235000019698 starch Nutrition 0.000 claims abstract description 11
- 229910052500 inorganic mineral Inorganic materials 0.000 claims abstract description 8
- 238000009360 aquaculture Methods 0.000 claims abstract description 7
- 244000144974 aquaculture Species 0.000 claims abstract description 7
- 239000000203 mixture Substances 0.000 claims description 62
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 36
- 239000000843 powder Substances 0.000 claims description 26
- 238000002156 mixing Methods 0.000 claims description 14
- 241000251468 Actinopterygii Species 0.000 claims description 13
- 238000003756 stirring Methods 0.000 claims description 12
- 230000000694 effects Effects 0.000 claims description 10
- HEMHJVSKTPXQMS-UHFFFAOYSA-M Sodium hydroxide Chemical compound [OH-].[Na+] HEMHJVSKTPXQMS-UHFFFAOYSA-M 0.000 claims description 9
- 238000001914 filtration Methods 0.000 claims description 9
- 239000003755 preservative agent Substances 0.000 claims description 9
- 230000002335 preservative effect Effects 0.000 claims description 9
- 239000007787 solid Substances 0.000 claims description 9
- 239000011521 glass Substances 0.000 claims description 8
- 239000000126 substance Substances 0.000 claims description 8
- 238000000151 deposition Methods 0.000 claims description 6
- 230000008021 deposition Effects 0.000 claims description 6
- 235000012054 meals Nutrition 0.000 claims description 6
- 238000001035 drying Methods 0.000 claims description 4
- 238000003760 magnetic stirring Methods 0.000 claims description 4
- FRXSZNDVFUDTIR-UHFFFAOYSA-N 6-methoxy-1,2,3,4-tetrahydroquinoline Chemical compound N1CCCC2=CC(OC)=CC=C21 FRXSZNDVFUDTIR-UHFFFAOYSA-N 0.000 claims description 3
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 3
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 3
- 229910001576 calcium mineral Inorganic materials 0.000 claims description 3
- 238000005119 centrifugation Methods 0.000 claims description 3
- 235000015165 citric acid Nutrition 0.000 claims description 3
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid group Chemical class C(CC(O)(C(=O)O)CC(=O)O)(=O)O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims description 3
- -1 compound amino acids Chemical class 0.000 claims description 3
- 235000013312 flour Nutrition 0.000 claims description 3
- 238000010438 heat treatment Methods 0.000 claims description 3
- 238000011017 operating method Methods 0.000 claims description 3
- 229910052760 oxygen Inorganic materials 0.000 claims description 3
- 239000001301 oxygen Substances 0.000 claims description 3
- 229910052573 porcelain Inorganic materials 0.000 claims description 3
- 238000001556 precipitation Methods 0.000 claims description 3
- 230000035484 reaction time Effects 0.000 claims description 3
- 230000007306 turnover Effects 0.000 claims description 3
- 239000000463 material Substances 0.000 claims 1
- 230000008901 benefit Effects 0.000 abstract description 5
- 230000004083 survival effect Effects 0.000 abstract description 2
- 241001439627 Schistostoma Species 0.000 description 8
- 210000001015 abdomen Anatomy 0.000 description 8
- RWSXRVCMGQZWBV-WDSKDSINSA-N glutathione Chemical compound OC(=O)[C@@H](N)CCC(=O)N[C@@H](CS)C(=O)NCC(O)=O RWSXRVCMGQZWBV-WDSKDSINSA-N 0.000 description 6
- 230000036039 immunity Effects 0.000 description 6
- 238000012360 testing method Methods 0.000 description 6
- 102000016943 Muramidase Human genes 0.000 description 5
- 108010014251 Muramidase Proteins 0.000 description 5
- 108010062010 N-Acetylmuramoyl-L-alanine Amidase Proteins 0.000 description 5
- 102000019197 Superoxide Dismutase Human genes 0.000 description 5
- 108010012715 Superoxide dismutase Proteins 0.000 description 5
- 239000004325 lysozyme Substances 0.000 description 5
- 229960000274 lysozyme Drugs 0.000 description 5
- 235000010335 lysozyme Nutrition 0.000 description 5
- 241000607528 Aeromonas hydrophila Species 0.000 description 4
- 241000193830 Bacillus <bacterium> Species 0.000 description 4
- 150000004676 glycans Chemical class 0.000 description 4
- 210000002966 serum Anatomy 0.000 description 4
- 230000008859 change Effects 0.000 description 3
- 230000000052 comparative effect Effects 0.000 description 3
- 229960003180 glutathione Drugs 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 244000247812 Amorphophallus rivieri Species 0.000 description 2
- 235000001206 Amorphophallus rivieri Nutrition 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 2
- ZNZYKNKBJPZETN-WELNAUFTSA-N Dialdehyde 11678 Chemical compound N1C2=CC=CC=C2C2=C1[C@H](C[C@H](/C(=C/O)C(=O)OC)[C@@H](C=C)C=O)NCC2 ZNZYKNKBJPZETN-WELNAUFTSA-N 0.000 description 2
- 239000003963 antioxidant agent Substances 0.000 description 2
- 230000003078 antioxidant effect Effects 0.000 description 2
- 230000002255 enzymatic effect Effects 0.000 description 2
- 230000000968 intestinal effect Effects 0.000 description 2
- 210000004347 intestinal mucosa Anatomy 0.000 description 2
- 229940025902 konjac mannan Drugs 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 239000012258 stirred mixture Substances 0.000 description 2
- 241000186000 Bifidobacterium Species 0.000 description 1
- WQZGKKKJIJFFOK-QTVWNMPRSA-N D-mannopyranose Chemical compound OC[C@H]1OC(O)[C@@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-QTVWNMPRSA-N 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 102000006587 Glutathione peroxidase Human genes 0.000 description 1
- 108700016172 Glutathione peroxidases Proteins 0.000 description 1
- 102000018697 Membrane Proteins Human genes 0.000 description 1
- 108010052285 Membrane Proteins Proteins 0.000 description 1
- 240000007594 Oryza sativa Species 0.000 description 1
- 235000007164 Oryza sativa Nutrition 0.000 description 1
- 102000003992 Peroxidases Human genes 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 230000003026 anti-oxygenic effect Effects 0.000 description 1
- 230000000840 anti-viral effect Effects 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000004071 biological effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000003912 environmental pollution Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 210000001035 gastrointestinal tract Anatomy 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 229930182478 glucoside Natural products 0.000 description 1
- 150000008131 glucosides Chemical class 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 230000001900 immune effect Effects 0.000 description 1
- 230000003053 immunization Effects 0.000 description 1
- 238000002649 immunization Methods 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 210000000936 intestine Anatomy 0.000 description 1
- 239000000252 konjac Substances 0.000 description 1
- 210000003750 lower gastrointestinal tract Anatomy 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 229920005615 natural polymer Polymers 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- 230000001717 pathogenic effect Effects 0.000 description 1
- 108040007629 peroxidase activity proteins Proteins 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 235000013406 prebiotics Nutrition 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 238000012545 processing Methods 0.000 description 1
- 235000009566 rice Nutrition 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
- A01K61/00—Culture of aquatic animals
- A01K61/10—Culture of aquatic animals of fish
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/20—Animal feeding-stuffs from material of animal origin
- A23K10/22—Animal feeding-stuffs from material of animal origin from fish
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/105—Aliphatic or alicyclic compounds
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/158—Fatty acids; Fats; Products containing oils or fats
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/163—Sugars; Polysaccharides
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/26—Compounds containing phosphorus
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/80—Feeding-stuffs specially adapted for particular animals for aquatic animals, e.g. fish, crustaceans or molluscs
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
- Y02A40/818—Alternative feeds for fish, e.g. in aquacultures
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02P—CLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
- Y02P60/00—Technologies relating to agriculture, livestock or agroalimentary industries
- Y02P60/80—Food processing, e.g. use of renewable energies or variable speed drives in handling, conveying or stacking
- Y02P60/87—Re-use of by-products of food processing for fodder production
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Animal Husbandry (AREA)
- Food Science & Technology (AREA)
- Marine Sciences & Fisheries (AREA)
- Molecular Biology (AREA)
- Environmental Sciences (AREA)
- Biotechnology (AREA)
- Physiology (AREA)
- Health & Medical Sciences (AREA)
- Insects & Arthropods (AREA)
- Biodiversity & Conservation Biology (AREA)
- Biomedical Technology (AREA)
- Birds (AREA)
- Botany (AREA)
- Mycology (AREA)
- Inorganic Chemistry (AREA)
- Fodder In General (AREA)
- Feed For Specific Animals (AREA)
Abstract
The present invention relates to a kind of fish meal for improving breeding efficiency and preparation method thereof, feeding methods, the fish meal for improving breeding efficiency are disclosed, by following parts by weight of component：1 ~ 3 part of 35 ~ 45 parts of fish meal, 16 ~ 24 parts of dregs of beans, 10 ~ 12 parts of starch, 1 ~ 4 part of rape oil, 0.8 ~ 1.5 part of calcium dihydrogen phosphate, 1 ~ 2 part of minerals, 0.8 ~ 1.2 part of choline, 80 ~ 100 parts of water and konjak glucomannan.Fish meal provided by the invention, konjak glucomannan is added in feed, and after feeding neat mouth schizothoracin, immune performance is remarkably reinforced, and survival rate significantly improves, and effectively improves economic benefit, reduces aquaculture cost.
Description
Technical field
The present invention relates to biological feedstuff technical fields, and in particular to a kind of fish meal for improving breeding efficiency and its preparation side
Method, feeding method.
Background technology
Neat mouth schizothoracin immunity is relatively low, more sensitive to environmental stimuli, causes fish due to environmental pollution etc. in recent years
Class stock number is drastically reduced, and fisherman's economy is caused to be damaged, and the flesh of fish market price is risen sharply.Therefore, urgent need exploitation is a kind of to carry
The feed of high neat schistostoma abdomen para-immunity performance.
Konjak glucomannan is also known as konjac polysaccharide, and 1.6 are pressed by D~mannose and D~glucose:1 ratio is with β-Isosorbide-5-Nitrae
Glucosides key connection, is a kind of natural polymer heteroglycan of known viscosity maximum, and konjak glucomannan is a kind of immune tune
Agent is saved, plays an important role of to improve immunocompetence, mainly be realized by following two approach：First, by with being distributed in intestinal villus
Cell surface protein receptors between interaction play immunization；Second is that by some on intestinal mucosa to being distributed in
Cell signal, which intervene, adjusts immunity of organism.Promote in addition, konjak glucomannan is alternatively arranged as a kind of exogenous prebiotics
The growth and breeding of beneficial microbe such as Bifidobacterium in enteron aisle, so as to inhibit the growth and breeding of pathogen, play raising indirectly
The effect of immunity of organisms.Konjak glucomannan can be in the film attachment that intestinal tract surface formation pore size does not wait after entering enteron aisle
In intestinal wall, the intrusion of harmful substance is hindered, so as to build up resistance.
The konjak glucomannan of neat mouth schizothoracin immune performance that can improve a kind of at present is in fish meal currently on the market
Application almost without, therefore, neat mouth schizothoracin is raised into fish meal by adding konjak glucomannan, to exploitation can carry
The feed of high fish immunity performance has broad prospects.
Another reason for neat schistostoma abdomen stock of fish amount is caused drastically to reduce, is that feeding method is improper, therefore, is badly in need of
A kind of specification feeding method improves the culture benefit of neat mouth schizothoracin.
The content of the invention
The technical problem to be solved by the present invention is to neat schistostoma abdomen fish culture technical deficiencies, provide a kind of raising breeding efficiency
Fish meal.The fish meal can be effectively improved the immunocompetence of neat mouth schizothoracin, improve breeding efficiency, fill up konjac mannan
Glycan is preparing the technological gap in improving fish immunity performance feeds.
Another object of the present invention is to provide a kind of preparation method for improving breeding efficiency fish meal.
Another object of the present invention is to provide a kind of feeding method for improving breeding efficiency fish meal.
In order to overcome the shortage of prior art, it is proposed that a kind of fish meal and feeding method for improving breeding efficiency.
The present invention is achieved by the following technical solutions：
A kind of fish meal for improving breeding efficiency disclosed by the invention, by following parts by weight of component：35~45 parts of fish meal, beans
16~24 parts of the dregs of rice, 10~12 parts of starch, 1~4 part of rape oil, 0.8~1.5 part of calcium dihydrogen phosphate, 1~2 part of minerals, choline 0.8
1~3 part of~1.2 parts, 80~100 parts of water and konjak glucomannan.
Further, the preparation method of the konjak glucomannan comprises the following steps：
S1. 100g konjaku flours are weighed to be dissolved in the ethanol solution of pre-configured 25ml 50% and stir evenly, are formed mixed
Close object A；
S2. the container for holding mixture A is sealed with preservative film, then is stirred mixture A with magnetic stirring apparatus
It mixes 35 minutes, the 10th minute, the 20th minute, the 30th minute in whipping process is separately added into 30%H2O27ml, 7ml, 3.5ml,
Add in 30%H2O2When need to open preservative film, covered again after addition, add in 17.5ml30%H in total2O2, mixed
Object B；
S3. mixture B is put into thermostat water bath together together with container, temperature is 40 DEG C, and height of water level is just
Height of the mixture B in container is not crossed, container is sealed with preservative film, and water bath time 4h is mixed
Object C；
S4. after water-bath, mixture C is filtered with filter device, then by the white solid in Buchner funnel
Powdered rubber is put into 50% ethanol solution and washs, and 50% ethanol consumption is 2 times of mixture C volume here, uses magnetic force
Blender is stirred 1 minute, then mixture is filtered；
S5. the repeatedly operating procedure of D 2 times, obtain white solid powder substance D；
S6. white solid powder substance D is put into clean beaker, adds in 5L citric acids, 50g compound amino acids use glass
After glass stick stirs evenly, the heating stirring 10min on electric furnace obtains the slightly transparent mixture E of white；
S7. mixture E is averagely poured into the conical flask of 20 500ml, is then sealed with tampon, then put conical flask
Enter in pressure cooker, temperature is 115 DEG C, and the reaction time is 15~25 minutes, and taking-up obtains mixture F；
S8. in desinfection chamber, the pH value of mixture F is transferred to 4.5~5.0 with the NaOH of 0.5mol/L, is then cooled to
At 28~30 DEG C, 0.75g~1g high activity dried yeasts, shaking table culture 48h are inoculated in each conical flask, cultivation temperature is 28~30
DEG C, rotating speed is 180~200r/min.Culture terminates, and culture is put into centrifuge and is centrifuged off thalline, and centrifuge speed is
5000~6000r/min, 6~8min of centrifugation time obtain mixture G；
S9. 95% concentration ethanol that two volumes are poured into mixture G is stirred precipitation, obtains white depositions H,
H is collected by filtration into clean beaker, 95% concentration ethanol for continuously adding two volumes is stirred, filtering；
S10. white depositions H is washed with 95% concentration ethanol of two volumes after filtering 5 times, you can obtain sample；
S11. gained sample in step J is placed in clean porcelain dish, it is 0.3~0.5cm that sample, which stacks thickness, is first placed in 60
Dry 8h in DEG C air dry oven, every half an hour in drying process, with glass bar by sample I carries out it is upper and lower turn over once, finally
Obtain the konjak glucomannan of high-purity.
A kind of preparation method for improving breeding efficiency fish meal is provided, is comprised the following steps：
Y1. dregs of beans is crushed, adds in fish meal thereto and starch carries out abundant mixing, obtain powders mixture one；
Y2. konjak glucomannan, calcium dihydrogen phosphate, minerals and choline are subjected to abundant mixing, obtain powder mixing
Object two；
Y3. the powders mixture one in step Y1 is divided into three parts, adds in the powders mixture that step Y2 is obtained in batches
It in two, is sufficiently stirred, after mixing, obtains powders mixture three；
Y4. rape oil is added in into the powders mixture three that step Y3 is obtained and is sufficiently stirred mixing, add in suitable water stirring,
It is soft or hard suitable so that presentation is homogeneous, hand pinch it is agglomerating after be not easy the mixture to scatter, mixture is then put into preparation
In the machine of fish meal, granulated meal is made；
Y5. the obtained granulated meals of step Y4 are put into feed baking oven dry to get.
A kind of feeding method for improving breeding efficiency fish meal is provided, weighed daily with neat schistostoma abdomen fish body 2.5% amount into
Row is fed, and the feed that daily requirement is fed is divided into 3 parts, in the morning 8 points~9 points respectively, 1 point~2 points of noon, 7 points at night
~8 points are fed, and water temperature control is in 15~22 DEG C, water quality PH：6.8~7.5, water body dissolved oxygen amount is in more than 3.5mg/L, thoroughly
Lightness is more than 35cm, and aquaculture pond has certain miniflow water, and streamflow carries out aquaculture pond blowdown 1 at 5 points~6 points for every afternoon
It is secondary, change water 1 time, water body is that cultivation density is 40 tails/m2~50 tails/m2。
Compared with prior art, the invention has the advantages that：
Fish meal provided by the invention, konjak glucomannan is added in feed, after feeding neat mouth schizothoracin, neat mouth
In schizothoracin body serum IgM increased activity, ACH50 and lysozyme content significantly improve, superoxide dismutase enzymatic activity, paddy Guang
Sweet peptide peroxidase activity improves, and mda content reduces, preceding middle hindgut Aeromonas hydrophila and the relatively total bacterium of bacillus
Content dramatically increase, after neat mouth schizothoracin eats the feed, immune performance is remarkably reinforced, and survival rate significantly improves, and effectively carries
High economic benefit reduces aquaculture cost.
Konjak glucomannan selected by the present invention has the characteristics that molecular weight is small, easy absorption, has antiviral, antioxygen
The multiple biological activities such as change, immunological regulation, have good biological compatibility and side effect is smaller, meet novel green additive
Requirement.
The feed, which is eaten for a long time, in neat schistostoma abdomen fish can improve immune performance, reduce drug dose, improve flesh quality, from
And flesh of fish security is improved, increase culture benefit.
Specific embodiment
It is further illustrated the present invention with reference to specific embodiment.Unless stated otherwise, used in the embodiment of the present invention
Routinely raw material purchased in market and conventional use of method, used equipment routinely set for this field for this field for raw material and method
It is standby.
Embodiment 1
1. a kind of fish meal for improving breeding efficiency, including following parts by weight of component：35 parts of fish meal, 16 parts of dregs of beans, starch
12 parts, 1 part of rape oil, 0.8 part of calcium dihydrogen phosphate, 1 part of minerals, 3 parts of 0.8 part of choline, 80 parts of water and konjak glucomannan.
Wherein, the preparation method of konjak glucomannan is as follows：
S1. 100g konjaku flours are weighed to be dissolved in the ethanol solution of pre-configured 25ml 50% and stir evenly, are formed mixed
Close object A；
S2. the container for holding mixture A is sealed with preservative film, then is stirred mixture A with magnetic stirring apparatus
It mixes 35 minutes, the 10th minute, the 20th minute, the 30th minute in whipping process is separately added into 30%H2O27ml, 7ml, 3.5ml,
Add in 30%H2O2When need to open preservative film, covered again after addition, add in 17.5ml30%H in total2O2, mixed
Object B；
S3. mixture B is put into thermostat water bath together together with container, temperature is 40 DEG C, and height of water level is just
Height of the mixture B in container is not crossed, container is sealed with preservative film, and water bath time 4h is mixed
Object C；
S4. after water-bath, mixture C is filtered with filter device, then by the white solid in Buchner funnel
Powdered rubber is put into 50% ethanol solution and washs, and 50% ethanol consumption is 2 times of mixture C volume here, uses magnetic force
Blender is stirred 1 minute, then mixture is filtered；
S5. the repeatedly operating procedure of D 2 times, obtain white solid powder substance D；
S6. white solid powder substance D is put into clean beaker, adds in 5L citric acids, 50g compound amino acids use glass
After glass stick stirs evenly, the heating stirring 10min on electric furnace obtains the slightly transparent mixture E of white；
S7. mixture E is averagely poured into the conical flask of 20 500ml, is then sealed with tampon, then put conical flask
Enter in pressure cooker, temperature is 115 DEG C, and the reaction time is 15~25 minutes, and taking-up obtains mixture F；
S8. in desinfection chamber, the pH value of mixture F is transferred to 4.5~5.0 with the NaOH of 0.5mol/L, is then cooled to
At 28~30 DEG C, 0.75g~1g high activity dried yeasts, shaking table culture 48h are inoculated in each conical flask, cultivation temperature is 28~30
DEG C, rotating speed is 180~200r/min.Culture terminates, and culture is put into centrifuge and is centrifuged off thalline, and centrifuge speed is
5000~6000r/min, 6~8min of centrifugation time obtain mixture G；
S9. 95% concentration ethanol that two volumes are poured into mixture G is stirred precipitation, obtains white depositions H,
H is collected by filtration into clean beaker, 95% concentration ethanol for continuously adding two volumes is stirred, filtering；
S10. white depositions H is washed with 95% concentration ethanol of two volumes after filtering 5 times, you can obtain sample；
S11. gained sample in step J is placed in clean porcelain dish, it is 0.3~0.5cm that sample, which stacks thickness, is first placed in 60
Dry 8h in DEG C air dry oven, every half an hour in drying process, with glass bar by sample I carries out it is upper and lower turn over once, finally
Obtain the konjak glucomannan of high-purity.
2. the preparation method of above-mentioned raising breeding efficiency fish meal, comprises the following steps：
Y1. dregs of beans is crushed, adds in fish meal thereto and starch carries out abundant mixing, obtain powders mixture one；
Y2. konjak glucomannan, calcium dihydrogen phosphate, minerals and choline are subjected to abundant mixing, obtain powder mixing
Object two；
Y3. the powders mixture one in step Y1 is divided into three parts, adds in the powders mixture that step Y2 is obtained in batches
It in two, is sufficiently stirred, after mixing, obtains powders mixture three；
Y4. rape oil is added in into the powders mixture three that step Y3 is obtained and is sufficiently stirred mixing, add in suitable water stirring,
It is soft or hard suitable so that presentation is homogeneous, hand pinch it is agglomerating after be not easy the mixture to scatter, mixture is then put into preparation
In the machine of fish meal, granulated meal is made；
Y5. the obtained granulated meals of step Y4 are put into feed baking oven dry to get.
3. the feeding method of above-mentioned raising breeding efficiency fish meal is as follows：
2.5% amount is weighed with neat schistostoma abdomen fish body daily to be fed, the feed that daily requirement is fed is divided into 3 parts,
In the morning 8 points~9 points respectively, 1 point~2 points of noon is fed for 7 points~8 points at night, and water temperature control is at 15~22 DEG C, water quality
PH is：6.8~7.5, for water body dissolved oxygen amount in more than 3.5mg/L, transparency is more than 35cm, and aquaculture pond has certain miniflow
Water, streamflow carry out aquaculture pond blowdown 1 time at 5 points~6 points for every afternoon, change water 1 time, water body is that cultivation density is 40 tails/m2
~50 tails/m2。
Embodiment 2
The present embodiment is different from part and is, the fish meal includes following parts by weight of component with reference to embodiment 1：Fish
45 parts of powder, 24 parts of dregs of beans, 10 parts of starch, 4 parts of rape oil, 1.5 parts of calcium dihydrogen phosphate, 2 parts of minerals, 1.2 parts of choline, 100 parts of water
With 1 part of konjak glucomannan.
Embodiment 3
The present embodiment is different from part and is, including following parts by weight of component with reference to embodiment 1：40 parts of fish meal, dregs of beans
22 parts, 10 parts of starch, 2 parts of rape oil, 1.0 parts of calcium dihydrogen phosphate, 2 parts of minerals, 1.2 parts of choline, 100 parts of water and konjac mannan
3 parts of glycan.
Embodiment 4
The present embodiment is different from part and is, including following parts by weight of component with reference to embodiment 1：42 parts of fish meal, dregs of beans
20 parts, 11 parts of starch, 2 parts of rape oil, 1.2 parts of calcium dihydrogen phosphate, 1.5 parts of minerals, 0.8 part of choline, 88 parts of water and Amorphophallus rivieri glucomannan
Reveal 2.4 parts of glycan.
Comparative example 1
This comparative example is different from part and is, including following parts by weight of component with reference to embodiment 1：35 parts of fish meal, dregs of beans
16 parts, 12 parts of starch, 1 part of rape oil, 0.8 part of calcium dihydrogen phosphate, 1 part of minerals, 0.8 part of choline, 80 parts of water.Randomly select 500
Tail average weight is that the neat mouth schizothoracin of physical health of 83 ± 2.14g is raised, with carrying for Examples 1 to 4 and comparative example 1
The fish meal of high breeding efficiency is as experimental group 1~4 and control group, the neat mouth schizothoracin of every group of 100 tails, after raising 80 days,
1~4 group of experiment and the neat mouth schizothoracin of control group are sampled respectively, and each group fish meal alignment mouth schizothoracin is immunized and
Antioxidant Indexes are (including serum IgM activity, ACH50, lysozyme (LZM) content, superoxide dismutase (SOD) enzymatic activity, third
Dialdehyde (MDA) content, glutathione peroxidase (GSH~PX) content) in vivo Aeromonas hydrophila, bacillus content into
Row detection.Testing result is shown in Table 1~2.
1 neat mouthful of schizothoracin of table is immunized and Antioxidant Indexes testing result
| Index | Test 1 group | Test 2 groups | Test 3 groups | Test 4 groups | Control group |
| IgM(μg/mL) | 1.68 | 1.73 | 1.76 | 1.83 | 1.35 |
| ACH50(U/mL) | 39.24 | 38.49 | 47.07 | 49.15 | 29.06 |
| LZM(U/mL) | 123.52 | 126.70 | 128.04 | 134.16 | 108.42 |
| SOD(U/mL) | 43.47 | 47.67 | 51.16 | 55.43 | 52.16 |
| MDA(nmol/mL) | 15.42 | 14.36 | 13.05 | 12.40 | 25.66 |
| GSH~PX (U/mL) | 463.58 | 478.88 | 486.84 | 508.49 | 320.31 |
2 neat mouthfuls of schizothoracin enteron aisle Aeromonas hydrophilas of table, the content of the relatively total bacterium of bacillus
As shown in Table 1, various dose konjak glucomannan can improve IgM activity, ACH50 in neat schistostoma abdomen fish serum
Content and LZM contents illustrate that konjak glucomannan can improve the immune performance of neat mouth schizothoracin.Meanwhile various dose konjaku
Glucomannans improves SOD activity, GSH~PX contents in serum, reduces the content of MDA, illustrates konjak glucomannan energy
Improve the antioxygenic property of neat mouth schizothoracin.
As shown in Table 2, compared with the control group, after during various dose konjak glucomannan can be improved before neat mouth schizothoracin
Aeromonas hydrophila and bacillus relative amount in intestines illustrate that konjak glucomannan can be connect by enteron aisle letter and improve neat schistostoma
Abdomen fish immunity keeps intestinal mucosa integrality.
The present invention illustrates the detailed process equipment of the present invention and processing step, people in the art by above-described embodiment
Member is it should be appreciated that the present invention limits from above-described embodiment, and the above embodiments and description only describe the present invention
Principle, without departing from the spirit and scope of the present invention, various changes and modifications of the present invention are possible, this is to this field
It is it will be apparent that these changes and improvements all fall within the protetion scope of the claimed invention for technical staff.
Claims (4)
1. a kind of fish meal for improving breeding efficiency, which is characterized in that by following parts by weight of component：35 ~ 45 parts of fish meal, dregs of beans 16
~ 24 parts, 10 ~ 12 parts of starch, 1 ~ 4 part of rape oil, 0.8 ~ 1.5 part of calcium dihydrogen phosphate, 1 ~ 2 part of minerals, 0.8 ~ 1.2 part of choline, water
80 ~ 100 parts and 1 ~ 3 part of konjak glucomannan.
2. fish meal according to claim 1, which is characterized in that the preparation method of the konjak glucomannan includes following
Step：
S1. 100g konjaku flours are weighed to be dissolved in the ethanol solution of pre-configured 25ml50% and stir evenly, form mixture
A；
S2. the container for holding mixture A is sealed with preservative film, then mixture A is stirred 35 with magnetic stirring apparatus
Minute, it is separately added into 30%H within the 10th minute, the 20th minute, the 30th minute in whipping process2O27ml, 7ml, 3.5ml are added in
30%H2O2When need to open preservative film, covered again after addition, add in 17.5ml30%H in total2O2, obtain mixture B；
S3. mixture B is put into thermostat water bath together together with container, temperature is 40 DEG C, and height of water level did not had just
Height of the mixture B in container, container are sealed with preservative film, and water bath time 4h obtains mixture C；
S4. after water-bath, mixture C is filtered with filter device, then by the white solid powder in Buchner funnel
Substance is put into 50% ethanol solution and washs, and 50% ethanol consumption is 2 times of mixture C volume here, uses magnetic stirring apparatus
It is stirred 1 minute, then mixture is filtered；
S5. the repeatedly operating procedure of D 2 times, obtain white solid powder substance D；
S6. white solid powder substance D is put into clean beaker, adds in 5L citric acids, 50g compound amino acids use glass bar
After stirring evenly, the heating stirring 10min on electric furnace obtains the slightly transparent mixture E of white；
S7. mixture E is averagely poured into the conical flask of 20 500ml, is then sealed with tampon, conical flask is then put into height
It presses in pot, temperature is 115 DEG C, and the reaction time is 15 ~ 25 minutes, and taking-up obtains mixture F；
S8. in desinfection chamber, the pH value of mixture F is transferred to 4.5 ~ 5.0 with the NaOH of 0.5mol/L, is then cooled to 28 ~ 30
DEG C when, 0.75g ~ 1g high activity dried yeasts, shaking table culture 48h are inoculated in each conical flask, cultivation temperature is 28 ~ 30 DEG C, rotating speed
For 180 ~ 200r/min；Culture terminates, and culture is put into centrifuge and is centrifuged off thalline, centrifuge speed for 5000 ~
6000r/min, 6 ~ 8min of centrifugation time obtain mixture G；
S9. 95% concentration ethanol that two volumes are poured into mixture G is stirred precipitation, obtains white depositions H, filtering
H is collected into clean beaker, 95% concentration ethanol for continuously adding two volumes is stirred, filtering；
S10. white depositions H is washed with 95% concentration ethanol of two volumes after filtering 5 times, you can obtain sample；
S11. gained sample in step J is placed in clean porcelain dish, it is 0.3 ~ 0.5 cm that sample, which stacks thickness, is first placed in 60 DEG C of drums
Dry 8h in wind drying box, every half an hour in drying process, with glass bar by sample I carries out it is upper and lower turn over once, finally it is high
The konjak glucomannan of purity.
3. the preparation method of any one of a kind of claim 1 ~ 2 fish meal, which is characterized in that comprise the following steps：
Y1. dregs of beans is crushed, adds in fish meal thereto and starch carries out abundant mixing, obtain powders mixture one；
Y2. konjak glucomannan, calcium dihydrogen phosphate, minerals and choline are subjected to abundant mixing, obtain powders mixture two；
Y3. the powders mixture one in step Y1 is divided into three parts, added in batches in the powders mixture two that step Y2 is obtained,
It is sufficiently stirred, after mixing, obtains powders mixture three；
Y4. rape oil is added in into the powders mixture three that step Y3 is obtained and is sufficiently stirred mixing, add in suitable water stirring so that
Present it is homogeneous, it is soft or hard suitable, hand pinch it is agglomerating after be not easy the mixture to scatter, then by mixture be put into prepare fish raise
In the machine of material, granulated meal is made；
Y5. the obtained granulated meals of step Y4 are put into feed baking oven dry to get.
It is 4. a kind of such as the feeding method of any one of claim 1 ~ 2 fish meal, which is characterized in that daily with neat mouth schizothoracin
The amount of weight 2.5% is fed, and the feed that daily requirement is fed is divided into 3 parts, in the morning 8 points ~ 9 points respectively, and at 1 point at noon ~
It 2 points, is fed for 7 points ~ 8 points at night, water temperature control is in 15 ~ 22 DEG C, water quality PH：6.8 ~ 7.5, water body dissolved oxygen amount is in 3.5mg/
More than L, transparency are more than 35cm, and aquaculture pond has certain miniflow water, and streamflow is to be cultivated 5 points ~ 6 points of every afternoon
Pond blowdown 1 time, changes water 1 time, and water body is that cultivation density is 40 tails/m2~ 50 tails/m2。
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| CN110810636A (en) * | 2019-12-09 | 2020-02-21 | 无锡华诺威动物保健品有限公司 | Regulator for improving intestinal health of micropterus salmoides at feed conversion stage and preparation method and application thereof |
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2017
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110810636A (en) * | 2019-12-09 | 2020-02-21 | 无锡华诺威动物保健品有限公司 | Regulator for improving intestinal health of micropterus salmoides at feed conversion stage and preparation method and application thereof |
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