CN108094203A - A kind of preparation method of Panax notoginseng seeds - Google Patents
A kind of preparation method of Panax notoginseng seeds Download PDFInfo
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- CN108094203A CN108094203A CN201711402380.1A CN201711402380A CN108094203A CN 108094203 A CN108094203 A CN 108094203A CN 201711402380 A CN201711402380 A CN 201711402380A CN 108094203 A CN108094203 A CN 108094203A
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01H—NEW PLANTS OR NON-TRANSGENIC PROCESSES FOR OBTAINING THEM; PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES
- A01H4/00—Plant reproduction by tissue culture techniques ; Tissue culture techniques therefor
- A01H4/005—Methods for micropropagation; Vegetative plant propagation using cell or tissue culture techniques
- A01H4/006—Encapsulated embryos for plant reproduction, e.g. artificial seeds
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Abstract
The present invention provides a kind of preparation methods of Panax notoginseng seeds, include the following steps：The differentiation culture of Radix Notoginseng embryo callus is obtained into somatic embryo, culture medium composition is：+ 5 8g/L agar of 2,4 D+28 32g/L sucrose of SH+0.2 0.4mg/L；Somatic embryo is continued into differentiation culture and obtains ripe cotyledon shape embryo, culture medium composition is：+ 5 7g/L agar of SH+28 32g/L sucrose；Using the cotyledon shape embryo as embedding brood body, using SH+1 2wt% sucrose as artificial endosperm, with 3 5wt% sodium alginate behaviour work post scytoblastemas, using drip manufacturing artificial seed, you can.The preparation method of the Panax notoginseng seeds of the embodiment of the present invention solves the situation of seedling shortage in the cultivation of Radix Notoginseng large-scale artificial by using modern biotechnology, and Radix Notoginseng cells,primordial has the advantages of speed of growth is fast, and character is stablized.
Description
Technical field
The present invention relates to Radix Notoginseng to cultivate field, in particular to a kind of preparation method of Panax notoginseng seeds.
Background technology
Radix Notoginseng also known as pseudo-ginseng are Umbellales Araliaceae, are distributed mainly on the ground such as Yunnan, Guangxi, Jiangxi, Sichuan.In
Medicinal material Radix Notoginseng is using its root as medicinal effects, has the effect of dissipate stasis of blood hemostasis, detumescence ding-tong.Spitting of blood is cured mainly, is spitted blood, nosebleed
Blood is had blood in stool, uterine bleeding, traumatic hemorrhage, chest and abdomen shouting pain, falls servant's swelling and pain.
Radix Notoginseng is one of China's tradition rare traditional Chinese medicine, 40 kinds of large big medicinal material kinds, away from it is modern have more than 400 years should
It with history, at home and abroad has long enjoyed a good reputation, Radix Notoginseng just has the laudatory title of " hemostasis god's medicine " from ancient times.Due to Radix Notoginseng it is unique apparent the effect of,
Constantly by expansion purposes, health care, chemical industry, medical dosage persistently rise, and especially China enters cardiovascular and cerebrovascular disease high-incidence season, phase
It closes research and development product to emerge in an endless stream, and demand is huge, Guangxi Wuzhou tianqi toothpaste, Yunnan Baiyao group, Tianjin day bodyguard power, Guangzhou
White Cloud Mountain, Yuxi peacekeeping, Zhenbao Island pharmacy, Shanghai Hua Yudeng enterprises are all that the large user of Radix Notoginseng represents.Annual requirement has connect
Nearly 8000 tons, dosage is still in ascendant trend.
Increasingly increase with market demand, approaches to cultivation question is also into the emphasis of research.Conventionally employed rhizome point
The modes of reproduction breeding potential of strain and root induction seedling is very low, and production cost is very high, while is unfavorable for the protection of resource.Utilize tissue
The mode quickly bred is cultivated, although can solve the problems, such as quickly to breed to a certain extent, however, there are also callus
Be organized in after subculture mostly generation there is variation, germplasm is degenerated, transplantation of seedlings survival rate is low and the bottles such as production cost height for callus differentiation
Neck problem largely limits the large-scale cultivation production of Radix Notoginseng.
Artificial seed is in fact closely related with somatic embryo practical application.Somatic embryo have multiplication is fast, yield is high,
The advantages of inheritance stability, it is even more important that it also has bipolar structure, can settle at one go to form complete plant, no
Need the formation of routinely substep induced bud and root.Thus, artificial seed has a extensive future.It there is no both at home and abroad at present related
The relevant report of the comparison system of Radix Notoginseng artificial seed, also in the technological gap stage.
In view of this, it is special to propose the present invention.
The content of the invention
It is an object of the invention to provide a kind of preparation methods of Panax notoginseng seeds, and three are solved by using modern biotechnology
The situation of seedling shortage in the cultivation of seven large-scale artificials, Radix Notoginseng cells,primordial have the advantages of speed of growth is fast, and character is stablized, gram
It has taken easy browning in Radix Notoginseng conventional organization culture, the defects of variation and production cycle are long, of high cost, while has also solved Radix Notoginseng kind
The problem of son is measured less, low reproduction rate opens a kind of new way for the cultivation of Panax notoginseng seeds, realizes Radix Notoginseng somatic embryo multiplication
Differentiation, last Radix Notoginseng artificial seed germination rate obtained can reach more than 95%.
In order to realize the above-mentioned purpose of the present invention, spy uses following technical scheme：
The present invention provides a kind of preparation methods of Panax notoginseng seeds, include the following steps：
(A) the differentiation culture of Radix Notoginseng embryo callus is obtained into somatic embryo, culture medium composition is：SH+0.2-0.4mg/L
2,4-D+28-32g/L sucrose+5-8g/L agar；
(B) somatic embryo is continued into differentiation culture and obtains ripe cotyledon shape embryo, culture medium composition is：SH+28-32g/L
Sucrose+5-7g/L agar；
(C) using the cotyledon shape embryo as embedding brood body, using SH+1-2wt% sucrose as artificial endosperm, with 3-5wt%
Sodium alginate behaviour work post scytoblastema, using drip manufacturing artificial seed, you can.
In the prior art, Radix Notoginseng is one of China's tradition rare traditional Chinese medicine, 40 kinds of large big medicinal material kinds, is had away from the present
The applicating history of more than 400 years, at home and abroad has long enjoyed a good reputation, and Radix Notoginseng just has the laudatory title of " hemostasis god's medicine " from ancient times.Since Radix Notoginseng is unique
The effect of apparent, constantly by expansion purposes, health care, chemical industry, medical dosage persistently rise, and especially China enters cardiovascular and cerebrovascular disease
The sick high-incidence season, correlation research and development product emerges in an endless stream, and demand is huge, Guangxi Wuzhou tianqi toothpaste, Yunnan Baiyao group, Tianjin
Its bodyguard power, Community in Baiyunshan, Guangzhou, Yuxi peacekeeping, Zhenbao Island pharmacy, Shanghai Hua Yudeng enterprises are all that the large user of Radix Notoginseng represents.Year needs
It measures already close to 8000 tons, dosage is still in ascendant trend.
Increasingly increase with market demand, approaches to cultivation question is also into the emphasis of research.Conventionally employed rhizome point
The modes of reproduction breeding potential of strain and root induction seedling is very low, and production cost is very high, while is unfavorable for the protection of resource.Utilize tissue
The mode quickly bred is cultivated, although can solve the problems, such as quickly to breed to a certain extent, however, there are also callus
Be organized in after subculture mostly generation there is variation, germplasm is degenerated, transplantation of seedlings survival rate is low and the bottles such as production cost height for callus differentiation
Neck problem largely limits the large-scale cultivation production of Radix Notoginseng.
Artificial seed is in fact closely related with somatic embryo practical application.Somatic embryo have multiplication is fast, yield is high,
The advantages of inheritance stability, it is even more important that it also has bipolar structure, can settle at one go to form complete plant, no
Need the formation of routinely substep induced bud and root.Thus, artificial seed has a extensive future.It there is no both at home and abroad at present related
The relevant report of the comparison system of Radix Notoginseng artificial seed, also in the technological gap stage.
In order to solve the above technical problems, The present invention provides a kind of preparation method of Panax notoginseng seeds, which leads to
After first by Radix Notoginseng embryo callus carry out Multiplying culture, then carry out differentiation culture again, finally using specific operation under the conditions of
Drip manufacturing artificial seed, step linking is closer before and after whole operation method, and flow standardizes very much, by using
The Panax notoginseng seeds that the preparation method of the artificial seed of the present invention is prepared compensate for the correlation technique of Radix Notoginseng artificial seed cultivation
Blank also overcomes easy browning in Radix Notoginseng conventional organization culture, the defects of variation and production cycle are long, of high cost.
Preferably, in the step (A) of the present invention, condition of culture is preferably light culture, and cultivation temperature is 20-24 DEG C, often
4-6 weeks on the differential medium subculture be more preferably that subculture was once on the differential medium in every 5 weeks once.
Somatic embryo in the step is preferably the somatic embryo in squamous subculture 3-4 generations.
Preferably, in the step (B) of the present invention, condition of culture equally carries out preferably under the conditions of light culture, culture temperature
Between 30-35 DEG C, the somatic embryo that can be turned out by controlling suitable condition of culture is easier to subsequently for degree control
The implementation of step.
Preferably, incubation time 50-60d is preferably 55d, and obtained cotyledon shape embryo length is cultivated by breaking up culture
It is preferably controlled between 4-6mm, more excellent is 5mm.
In the step (C) of the present invention, drip preferably carries out in accordance with the following steps：Cotyledon shape will be included using suction pipe
The semigel state sodium alginate of embryo drips to 5-6wt%CaCl2In solution, ion exchange 8-9min is cleaned to obtain the final product.
Wherein, for the drying of despumation, clean and cleaned preferably with distilled water, the number of cleaning is controlled in 2-
Between 3 times.
Preferably, cotyledon shape embryo and the sodium alginate are with 1-2：100 mass ratio is mixed.And after the two mixing
Heating stirring is preferably carried out material is thus formed above-mentioned semigel state, by measuring pH, pH value be preferably controlled in 6-7 it
Between.
The Panax notoginseng seeds being prepared by using the preparation method of the present invention are placed in 1/2SH+10g/L sucrose+6g/L fine jades
In the solid medium of fat, cultivation temperature is 25 DEG C, intensity of illumination 2000lx, illumination 16h/d, and germination rate is up to more than 95%.
Compared with prior art, beneficial effects of the present invention are：
(1) preparation method of Panax notoginseng seeds of the invention solves Radix Notoginseng large-scale artificial by using modern biotechnology
The situation of seedling shortage in cultivation, Radix Notoginseng cells,primordial have the advantages of speed of growth is fast, and character is stablized, and overcome Radix Notoginseng routine
Easy browning in tissue cultures, the defects of variation and production cycle are long, of high cost；
(2) preparation method of Panax notoginseng seeds of the invention also solves the problems, such as that Panax notoginseng seeds amount is few, low reproduction rate simultaneously,
A kind of new way is opened for the cultivation of Panax notoginseng seeds, realizes Radix Notoginseng somatic embryo Proliferation, Differentiation, last Radix Notoginseng people obtained
Work seed germination rate can reach more than 95%.
Description of the drawings
It in order to illustrate more clearly about the embodiment of the present invention or technical scheme of the prior art, below will be to embodiment or existing
There is attached drawing needed in technology description to be briefly described.
Fig. 1 is the specific appearance schematic diagram of the Panax notoginseng seeds obtained using the production method of the embodiment of the present invention 1；
Fig. 2 is that the Panax notoginseng seeds obtained using the production method of the embodiment of the present invention 2 grow the appearance schematic diagram after 20d；
Root and belemnoid break in the seed coat after Fig. 3 is the Panax notoginseng seeds growth 20d obtained using the production method of the embodiment of the present invention 2
Specific schematic diagram；
Fig. 4 is that the Panax notoginseng seeds obtained using the production method of the embodiment of the present invention 3 grow the appearance schematic diagram after 30d；
Fig. 5 is to start long shoot and leaf after the Panax notoginseng seeds obtained using the production method of the embodiment of the present invention 3 grow 30d
Specific schematic diagram；
Fig. 6 is that the Panax notoginseng seeds obtained using the production method of the embodiment of the present invention 4 grow the appearance schematic diagram after 60d.
Specific embodiment
Embodiment of the present invention is described in detail below in conjunction with embodiment, but those skilled in the art will
Understand, the following example is merely to illustrate the present invention, and is not construed as limiting the scope of the invention.It is not specified in embodiment specific
Condition person, the condition suggested according to normal condition or manufacturer carry out.Reagents or instruments used without specified manufacturer is
The conventional products that can be obtained by commercially available purchase.
Embodiment 1
The production method of Radix Notoginseng artificial seed is as follows：
(1) multiplication of somatic embryo：Embryo callus is connected on proliferated culture medium, culture medium composition is：SH+
0.2mg/L 2,4-D+28g/L sucrose+5g/L agar, every 4 weeks subculture 1 time, light culture, cultivation temperatures on identical culture medium
For 24 DEG C.Keep the differentiation capability of embryo callus；
(2) differentiation of somatic embryo：Embryo callus is accessed on somatic embryo differential medium, culture medium composition
For：SH+32g/L sucrose+7g/L agar, light culture, cultivation temperature are 30 DEG C.Ripe cotyledon shape embryo is obtained after 50d, and (length is about
4mm)；
(3) making of Radix Notoginseng artificial seed：Using the Radix Notoginseng somatic embryo of 4mm or so as embedding brood body, with SH+1% sugarcanes
Sugar is artificial endosperm, with 3wt% sodium alginate behaviour work post scytoblastema matter.Artificial seed making is carried out using drip：Ultra-clean
On workbench, body embryo is immersed in the artificial endosperm containing sodium alginate, the semigel state seaweed of body embryo will be surrounded by with suction pipe
Sour sodium drips to 5wt%CaCl2In solution, every artificial seed only wraps up a body embryo, and cotyledon shape embryo is with sodium alginate with 1：100
Mass ratio mixed.In CaCl2Effects of ion exchange 8min, cure balling-up after taking-up cleaned twice with distilled water, i.e.,
Obtain Radix Notoginseng artificial seed.It is placed in the solid medium of 1/2SH+10g/L sucrose+6g/L agar, cultivation temperature is 25 DEG C, light
It is 2000lx according to intensity, illumination 16h/d, for germination rate up to more than 95%, the shape that specific manual manufacture goes out Panax notoginseng seeds is shown in Fig. 1.
Embodiment 2
The production method of Radix Notoginseng artificial seed is as follows：
(1) multiplication of somatic embryo：Embryo callus is connected on proliferated culture medium, culture medium composition is：SH+
0.4mg/L 2,4-D+32g/L sucrose+8g/L agar, every 6 weeks subculture 1 time, light culture, cultivation temperatures on identical culture medium
For 20 DEG C.Keep the differentiation capability of embryo callus；
(2) differentiation of somatic embryo：Embryo callus is accessed on somatic embryo differential medium, culture medium composition
For：SH+28g/L sucrose+5g/L agar, light culture, cultivation temperature are 35 DEG C.Ripe cotyledon shape embryo is obtained after 50d, and (length is about
6mm)；
(3) making of Radix Notoginseng artificial seed：Using the Radix Notoginseng somatic embryo of 6mm or so as embedding brood body, with SH+1% sugarcanes
Sugar is artificial endosperm, with 3wt% sodium alginate behaviour work post scytoblastema matter.Artificial seed making is carried out using drip：Ultra-clean
On workbench, body embryo is immersed in the artificial endosperm containing sodium alginate, the semigel state seaweed of body embryo will be surrounded by with suction pipe
Sour sodium drips to 6wt%CaCl2In solution, every artificial seed only wraps up a body embryo, and cotyledon shape embryo is with sodium alginate with 2：100
Mass ratio mixed.In CaCl2Effects of ion exchange 9min, cure balling-up after taking-up cleaned three times with distilled water, i.e.,
Obtain Radix Notoginseng artificial seed.It is placed in the solid medium of 1/2SH+10g/L sucrose+6g/L agar, cultivation temperature is 25 DEG C, light
It is 2000lx according to intensity, illumination 16h/d, for germination rate up to more than 95%, specific Panax notoginseng seeds growth conditions are shown in Fig. 2-3.
Embodiment 3
The production method of Radix Notoginseng artificial seed is as follows：
(1) multiplication of somatic embryo：Embryo callus is connected on proliferated culture medium, culture medium composition is：SH+
0.3mg/L 2,4-D+30g/L sucrose+7g/L agar, every 5 weeks subculture 1 time, light culture, cultivation temperatures on identical culture medium
For 22 DEG C.Keep the differentiation capability of embryo callus；
(2) differentiation of somatic embryo：Embryo callus is accessed on somatic embryo differential medium, culture medium composition
For：SH+30g/L sucrose+6g/L agar, light culture, cultivation temperature are 32 DEG C.Ripe cotyledon shape embryo is obtained after 55d, and (length is about
5mm)；
(3) making of Radix Notoginseng artificial seed：Using the Radix Notoginseng somatic embryo of 5mm or so as embedding brood body, with SH+1% sugarcanes
Sugar is artificial endosperm, with 3wt% sodium alginate behaviour work post scytoblastema matter.Artificial seed making is carried out using drip：Ultra-clean
On workbench, body embryo is immersed in the artificial endosperm containing sodium alginate, the semigel state seaweed of body embryo will be surrounded by with suction pipe
Sour sodium drips to 6wt%CaCl2In solution, every artificial seed only wraps up a body embryo, and cotyledon shape embryo is with sodium alginate with 1.5：
100 mass ratio is mixed.In CaCl2Effects of ion exchange 9min, cure balling-up after taking-up cleaned three times with distilled water,
Obtain Radix Notoginseng artificial seed.It being placed in the solid medium of 1/2SH+10g/L sucrose+6g/L agar, cultivation temperature is 25 DEG C,
Intensity of illumination is 2000lx, and illumination 16h/d, for germination rate up to more than 95%, specific Panax notoginseng seeds growth conditions are shown in Fig. 4-5.
Embodiment 4
The production method of Radix Notoginseng artificial seed is as follows：
(1) multiplication of somatic embryo：Embryo callus is connected on proliferated culture medium, culture medium composition is：SH+
0.3mg/L 2,4-D+31g/L sucrose+6g/L agar, every 5 weeks subculture 1 time, light culture, cultivation temperatures on identical culture medium
For 23 DEG C.Keep the differentiation capability of embryo callus；
(2) differentiation of somatic embryo：Embryo callus is accessed on somatic embryo differential medium, culture medium composition
For：SH+30g/L sucrose+6g/L agar, light culture, cultivation temperature are 33 DEG C.Ripe cotyledon shape embryo is obtained after 60d, and (length is about
5mm)；
(3) making of Radix Notoginseng artificial seed：Using the Radix Notoginseng somatic embryo of 5mm or so as embedding brood body, with SH+1% sugarcanes
Sugar is artificial endosperm, with 3wt% sodium alginate behaviour work post scytoblastema matter.Artificial seed making is carried out using drip：Ultra-clean
On workbench, body embryo is immersed in the artificial endosperm containing sodium alginate, the semigel state seaweed of body embryo will be surrounded by with suction pipe
Sour sodium drips to 6wt%CaCl2In solution, every artificial seed only wraps up a body embryo, and cotyledon shape embryo is with sodium alginate with 1.5：
100 mass ratio is mixed.In CaCl2Effects of ion exchange 9min, cure balling-up after taking-up cleaned three times with distilled water,
Obtain Radix Notoginseng artificial seed.It being placed in the solid medium of 1/2SH+10g/L sucrose+6g/L agar, cultivation temperature is 25 DEG C,
Intensity of illumination is 2000lx, and illumination 16h/d, for germination rate up to more than 95%, specific Panax notoginseng seeds growth conditions are shown in Fig. 6.
Comparative example 1
Concrete operation step is consistent with embodiment 4, and simply no step (1)-(2), directly people is instilled with suction pipe by body embryo
In work endosperm.
Comparative example 2
Concrete operation step is consistent with embodiment 4, and simply in step (1), culture medium composition is：SH+0.6mg/L 2,4-D
+ 20g/L sucrose+2g/L agar.
Comparative example 3
Concrete operation step is consistent with embodiment 4, and simply in step (2), culture medium composition is：SH+35g/L sucrose+8g/
L agar.
Experimental example 1
Pulullan polysaccharide solution is sprayed on Radix Notoginseng artificial seed surface, is put into after to be dried in sterile triangular flask, with envelope
Membrana oralis is sealed, and is stored in 4 DEG C of refrigerator, each 50 seeds of embodiment alignment processing.
After 30 days, the solid that the artificial seed in each embodiment is inoculated into 1/2SH+10g/L sucrose+6g/L agar is trained
It supports in base, density is 1/cm2, depth 0.5cm, condition of culture is：Cultivation temperature is 25 DEG C, intensity of illumination 2000lx,
Illumination 16h/d, humidity 85% observe the conversion situation of seed, count germination rate after 30 days.Experimental result is shown in Table 1.
The statistics foundation of forward rate：Radix Notoginseng artificial seed is inoculated in fertile soil, after 1 month, statistics turns constant temperature incubation
Hair rate generates cotyledon and root as the successful standard of conversion using body embryo.
Concrete outcome see the table below shown in 1.
1 result of table counts
As can be seen that the preparation method of the Panax notoginseng seeds of the embodiment of the present invention can significantly improve germination rate from upper table 1,
Should be widely promoted application.
Compared with prior art, beneficial effects of the present invention are：
(1) preparation method of Panax notoginseng seeds of the invention solves Radix Notoginseng large-scale artificial by using modern biotechnology
The situation of seedling shortage in cultivation, Radix Notoginseng cells,primordial have the advantages of speed of growth is fast, and character is stablized, and overcome Radix Notoginseng routine
Easy browning in tissue cultures, the defects of variation and production cycle are long, of high cost；
(2) preparation method of Panax notoginseng seeds of the invention also solves the problems, such as that Panax notoginseng seeds amount is few, low reproduction rate simultaneously,
A kind of new way is opened for the cultivation of Panax notoginseng seeds, realizes Radix Notoginseng somatic embryo Proliferation, Differentiation, last Radix Notoginseng people obtained
Work seed germination rate can reach more than 95%.
Although illustrate and describing the present invention with specific embodiment, it will be appreciated that without departing substantially from the present invention's
Many other change and modification can be made in the case of spirit and scope.It is, therefore, intended that in the following claims
Including belonging to all such changes and modifications in the scope of the invention.
Claims (10)
1. a kind of preparation method of Panax notoginseng seeds, which is characterized in that include the following steps：
(A) the differentiation culture of Radix Notoginseng embryo callus is obtained into somatic embryo, culture medium composition is：SH+0.2-0.4mg/L 2,
4-D+29-32g/L sucrose+5-8g/L agar；
(B) somatic embryo is continued into differentiation culture and obtains ripe cotyledon shape embryo, culture medium composition is：SH+28-32g/L sucrose+
5-7g/L agar；
(C) using the cotyledon shape embryo as embedding brood body, using SH+1-2wt% sucrose as artificial endosperm, with 3-5wt% seaweed
Sour sodium behaviour work post scytoblastema, using drip manufacturing artificial seed, you can.
2. the preparation method of Panax notoginseng seeds according to claim 1, which is characterized in that in the step (A), condition of culture
For light culture, cultivation temperature is 20-24 DEG C, and per 4-6 weeks, subculture was once on the differential medium.
3. the preparation method of Panax notoginseng seeds according to claim 2, which is characterized in that in the step (A), every 5 weeks
Subculture is once on the differential medium.
4. the preparation method of Panax notoginseng seeds according to claim 1, which is characterized in that in the step (A), the body is thin
Blastula is the somatic embryo in squamous subculture 3-4 generations.
5. according to the preparation method of claim 1-4 any one of them Panax notoginseng seeds, which is characterized in that in the step (B),
Condition of culture is light culture, and cultivation temperature is 30-35 DEG C.
6. the preparation method of Panax notoginseng seeds according to claim 5, which is characterized in that in the step (B), incubation time
It is preferably 55d for 50-60d.
7. according to the preparation method of claim 1-4 any one of them Panax notoginseng seeds, which is characterized in that in the step (B),
The length of the cotyledon shape embryo is 4-6mm, is preferably 5mm.
8. according to the preparation method of claim 1-4 any one of them Panax notoginseng seeds, which is characterized in that in the step (C),
The step of drip makes includes：The semigel state sodium alginate for including cotyledon shape embryo is dripped to by 5-6wt% using suction pipe
CaCl2In solution, ion exchange 8-9min is cleaned to obtain the final product.
9. the preparation method of Panax notoginseng seeds according to claim 8, which is characterized in that in the step (C), cleaning uses
Distilled water is cleaned, and the number of cleaning is 2-3 times.
10. the preparation method of Panax notoginseng seeds according to claim 8, which is characterized in that in the step (C), the son
Leaf embryo is with the sodium alginate with 1-2：100 mass ratio is mixed.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110199883A (en) * | 2019-07-11 | 2019-09-06 | 云南维和药业股份有限公司 | A kind of breeding method of Radix Notoginseng tissue-cultured seedling |
| CN110771508A (en) * | 2019-11-26 | 2020-02-11 | 大连大学 | Preparation method of artificial blueberry seeds |
| CN111869565A (en) * | 2020-07-16 | 2020-11-03 | 云南农业大学 | Culture method for propagation of green embryogenic callus of panax notoginseng |
| CN113317203A (en) * | 2021-06-28 | 2021-08-31 | 大连大学 | Pogostemon cablin artificial seed culture method |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102907318A (en) * | 2011-08-01 | 2013-02-06 | 东北林业大学 | Rapid propagation of notoginseng regenerated plant by using bioreactor to cultivate notoginseng somatic embryos |
| CN104686340A (en) * | 2015-02-22 | 2015-06-10 | 杨业容 | Method for establishing cell suspension culture system of gynura divaricate (L.) DC. |
| CN106069791A (en) * | 2016-08-25 | 2016-11-09 | 文山苗乡三七科技有限公司 | A kind of Radix Notoginseng embryonic callus induction cultural method |
-
2017
- 2017-12-22 CN CN201711402380.1A patent/CN108094203B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102907318A (en) * | 2011-08-01 | 2013-02-06 | 东北林业大学 | Rapid propagation of notoginseng regenerated plant by using bioreactor to cultivate notoginseng somatic embryos |
| CN104686340A (en) * | 2015-02-22 | 2015-06-10 | 杨业容 | Method for establishing cell suspension culture system of gynura divaricate (L.) DC. |
| CN106069791A (en) * | 2016-08-25 | 2016-11-09 | 文山苗乡三七科技有限公司 | A kind of Radix Notoginseng embryonic callus induction cultural method |
Non-Patent Citations (3)
| Title |
|---|
| 桂进等: "三七体细胞胚胎发生及人工种子研制", 《实验生物学报》 * |
| 郑惠元: "人参属代表植物人工种子包埋技术研究", 《中国优秀硕士学位论文全文数据库 农业科技辑》 * |
| 陶雷等: "五加科植物体细胞胚发生研究进展", 《中国农学通报》 * |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110199883A (en) * | 2019-07-11 | 2019-09-06 | 云南维和药业股份有限公司 | A kind of breeding method of Radix Notoginseng tissue-cultured seedling |
| CN110771508A (en) * | 2019-11-26 | 2020-02-11 | 大连大学 | Preparation method of artificial blueberry seeds |
| CN111869565A (en) * | 2020-07-16 | 2020-11-03 | 云南农业大学 | Culture method for propagation of green embryogenic callus of panax notoginseng |
| CN113317203A (en) * | 2021-06-28 | 2021-08-31 | 大连大学 | Pogostemon cablin artificial seed culture method |
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Application publication date: 20180601 Assignee: Guangzhou Fenghua Shubao Biotechnology Co.,Ltd. Assignor: INSTITUTE OF SPECIAL ANIMAL AND PLANT SCIENCES OF CAAS Contract record no.: X2023980032410 Denomination of invention: A preparation method of Panax notoginseng seeds Granted publication date: 20201124 License type: Common License Record date: 20230222 |