CN108088726B - Multifunctional optional dyeing device - Google Patents
Multifunctional optional dyeing device Download PDFInfo
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- CN108088726B CN108088726B CN201810129669.9A CN201810129669A CN108088726B CN 108088726 B CN108088726 B CN 108088726B CN 201810129669 A CN201810129669 A CN 201810129669A CN 108088726 B CN108088726 B CN 108088726B
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- 238000004043 dyeing Methods 0.000 title claims abstract description 241
- 238000010186 staining Methods 0.000 claims abstract description 132
- 239000007788 liquid Substances 0.000 claims abstract description 114
- 230000007246 mechanism Effects 0.000 claims abstract description 112
- 238000004140 cleaning Methods 0.000 claims abstract description 69
- 239000011521 glass Substances 0.000 claims abstract description 51
- 230000033001 locomotion Effects 0.000 claims abstract description 30
- 230000001174 ascending effect Effects 0.000 claims abstract description 8
- 239000012192 staining solution Substances 0.000 claims description 21
- 239000000243 solution Substances 0.000 claims description 19
- 238000000034 method Methods 0.000 claims description 14
- 238000013519 translation Methods 0.000 claims description 11
- 238000005406 washing Methods 0.000 claims description 5
- 239000012530 fluid Substances 0.000 abstract 1
- 230000008569 process Effects 0.000 description 8
- 239000000975 dye Substances 0.000 description 7
- 239000008280 blood Substances 0.000 description 5
- 210000004369 blood Anatomy 0.000 description 5
- 230000008859 change Effects 0.000 description 5
- 239000003153 chemical reaction reagent Substances 0.000 description 3
- 238000001514 detection method Methods 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 239000013589 supplement Substances 0.000 description 3
- 238000003794 Gram staining Methods 0.000 description 2
- 230000009471 action Effects 0.000 description 2
- 238000007599 discharging Methods 0.000 description 2
- 230000003834 intracellular effect Effects 0.000 description 2
- 238000005192 partition Methods 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 238000012546 transfer Methods 0.000 description 2
- 238000003759 clinical diagnosis Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 210000002751 lymph Anatomy 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000001502 supplementing effect Effects 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
- G01N1/31—Apparatus therefor
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- General Health & Medical Sciences (AREA)
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- Immunology (AREA)
- Pathology (AREA)
- Sampling And Sample Adjustment (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The application discloses a multifunctional optional dyeing device, which comprises a dyeing platform, a slide supporting structure and a slide moving mechanism, wherein the dyeing platform is provided with at least one cleaning area and at least two dyeing areas, and the cleaning area and the dyeing areas are respectively supplied with cleaning liquid and dyeing liquid; the glass slide supporting structures are arranged on two sides of the dyeing platform and used for supporting a glass slide to be horizontally placed above the dyeing platform and enabling the glass slide and the surface of the dyeing platform to form a gap; the slide moving mechanism is used for driving the slide to do ascending or descending or translational motion, so that the slide moves and optionally leaves or enters a certain dyeing area or a cleaning area. The application provides a multi-functional optional dyeing apparatus can send the sample of different grade type to the different dyeing district of dyeing platform and carry out dyeing treatment, still can shift certain specific slide in a certain dyeing district to the realization is dyed the different samples of demand of staining fluid of staining time or required use to same device.
Description
Technical Field
The application relates to the technical field of detection equipment, in particular to a multifunctional optional dyeing device.
Background
In the field of medical examination, the staining is an experimental mode of staining the tissues and the intracellular structures of a specimen to be detected respectively by one or more dyes, so that the intracellular structures can be clearly observed under a microscope, and the correct judgment of clinical diagnosis is facilitated.
Present specimen dyeing divide into manual dyeing and automatic dyeing, and automatic dyeing is accomplished by automatic dyeing apparatus, and current automatic dyeing apparatus adopts a plurality of dyeing boxes to dye every slide, and another kind of dyeing apparatus adopts the dyeing dish to dye a plurality of slides simultaneously, nevertheless above the device need set up multiple clamping part, moving part for remove dyeing box and smear, and the structure is complicated with high costs.
The applicant has proposed a dyeing device in the future, and the granted number is CN206546284U, in the dyeing device, a slide driving mechanism is a screw rod, the slide is driven by adopting a linear driving mode, each slide passes through each dyeing area and a cleaning area of the dyeing device in sequence, and only batch dyeing of the same type of samples can be realized, and the structure has a large requirement on the length and the size of a platform, and the volume of the dyeing device is increased.
Disclosure of Invention
To solve the above technical problems, a first object of the present invention is to provide a multifunctional optional dyeing apparatus; a second object of the invention is to provide a method for using the above-mentioned device. The multifunctional optional dyeing device provided by the invention can be used for delivering different types of samples to different dyeing areas of the dyeing platform for dyeing treatment, and can also be used for transferring a certain specific slide in a certain dyeing area, so that the samples with different dyeing time or different requirements on the used dyeing solution can be dyed by the same device.
The technical scheme provided by the invention is as follows:
a multifunctional optional dyeing device comprises a dyeing platform, a slide supporting structure and a slide moving mechanism,
the dyeing platform is provided with at least one cleaning area and at least two dyeing areas, and the cleaning area and the dyeing areas are respectively supplied with cleaning liquid and dyeing liquid;
the glass slide supporting structures are arranged on two sides of the dyeing platform and used for supporting a glass slide to be horizontally placed above the dyeing platform and enabling the glass slide and the surface of the dyeing platform to form a gap;
the slide moving mechanism is used for driving the slide to do ascending or descending or translational motion, so that the slide moves and optionally leaves or enters a certain dyeing area or a cleaning area.
Preferably, the slide moving mechanism includes a gripping member for gripping the slide, and a driving member for driving the gripping member to move.
Preferably, the slide moving mechanism comprises a slide tray, a rotation driving component and a lifting driving component, wherein the slide tray is arranged above the staining platform and used for supporting a slide, the rotation driving component drives the slide tray to rotate, and the lifting driving component drives the slide tray to move up and down in the vertical direction.
Preferably, the slide moving mechanism further comprises an upper and lower sheet member for moving the slide into and out of the slide tray.
Preferably, the slide tray is provided with at least one slide placing groove.
Preferably, the slide moving mechanism includes a clamping mechanism, a driving mechanism, a rotating tray, and a rotating driving mechanism, the rotating tray is disposed above the staining platform and used for supporting the slide, the rotating driving mechanism drives the rotating tray to rotate, the clamping mechanism is used for clamping the slide, and the driving mechanism is used for driving the clamping mechanism to move, so that the slide moves, leaves or contacts the rotating tray.
Preferably, the staining area comprises one or more staining sections, different staining solutions are respectively provided in different staining sections in the same staining area, and the slide moving mechanism drives the slide to move into different staining areas and to sequentially move horizontally into each staining section for staining.
Preferably, the cleaning area and the dyeing area of the dyeing platform, or each dyeing section in the dyeing area, are respectively provided with an independent working liquid output mechanism.
Preferably, the working liquid output mechanism comprises a liquid supply hole and a liquid supply groove, wherein the liquid supply hole is used for supplying working liquid, two ends of the liquid supply groove extend to the edge of the dyeing platform, and the liquid supply hole is arranged in the liquid supply groove.
The invention also provides a method of using the apparatus of any one of the above claims, comprising the steps of:
a. moving the slide to the position above a dyeing area needing to enter by using a slide moving mechanism, then moving the slide downwards, and flatly placing the slide on the slide supporting structure to enable the slide and the surface of the dyeing platform to form a gap;
b. a working liquid output mechanism is used for supplying dyeing liquid and cleaning liquid, and a slide moving mechanism is used for driving a slide to translate on a dyeing platform and is in full contact with the dyeing liquid;
c. after dyeing is finished, moving the slide upwards by using a slide moving mechanism, then translating the slide to move to a position above a cleaning area, moving the slide downwards, and flatly placing the slide on the slide supporting structure to form a gap between the slide and the surface of the dyeing platform so that the slide is fully contacted with a cleaning solution;
d. and after cleaning, taking out the slide by using a slide moving mechanism.
The existing staining device can only stain the same specimen in batch, and each slide is stained by each staining area and a cleaning area of the staining device in sequence. In practical application, a plurality of small and medium hospitals have small single sample amount and small batch dyeing requirement, but different samples (such as gynecology, blood and the like) need to be dyed. In the patent previously filed by the applicant, the slide can only be stained by a pipeline mode through each staining area of the staining platform, and different staining areas can not be selected for staining different staining requirements of each sample.
Aiming at the problems in the prior art, the invention provides a multifunctional optional dyeing device which can realize dyeing of different samples in different modes, and different dyeing areas are selected to dye the samples according to the dyeing requirements of the samples. The invention provides a multifunctional optional dyeing device which comprises a dyeing platform, a glass slide supporting structure and a glass slide moving mechanism, wherein the glass slide supporting structure is arranged on two sides of the dyeing platform, supports a glass slide to be horizontally placed above the dyeing platform, and enables the glass slide and the surface of the dyeing platform to form a gap. In the present invention, the surface of the staining stage refers to a liquid level formed by a staining solution or a cleaning solution contained in the staining stage, and the liquid level is located below the slide. Due to the action of the surface tension of the liquid, the dyeing liquid or the cleaning liquid can form a liquid film on the surface of the dyeing platform, and the height of the liquid surface of the liquid film exceeds the height of the glass slide, so that the dyeing liquid or the cleaning liquid in the dyeing platform can be soaked into a sample on the glass slide to dye or clean the sample.
Simultaneously, the dyeing platform is equipped with two at least dyeing districts, and with at least one washing district, each dyeing district provides different staining solution, and the washing district provides the washing liquid, washs unnecessary staining solution on the slide. The staining platform can be provided with 2-3 staining areas (such as Ruhrstan's staining areas and gram staining areas), or the number of the staining areas can be flexibly set according to the actual sample staining requirements. Different staining areas can be provided for different staining solutions to stain different samples, and the slide moving mechanism moves the slide to the corresponding staining areas to stain the slide, so that the technical requirement of optionally staining various samples is met. Different dyeing areas are separated from each other through a partition plate, and different working liquids are prevented from influencing each other.
The slide moving mechanism is arranged to drive the slide to move up or down or move in a translation mode so as to move the slide. The lifting motion means that the slide moving mechanism drives the slide to lift so as to enable the slide to be separated from the staining platform and the slide supporting structure by a certain distance; the descending motion means that the slide moving mechanism drives the slide to descend so as to enable the slide to be in contact with the slide supporting structure; the translational motion includes two types, one is that the slide moving mechanism drives the slide to translate without contacting the slide support structure (i.e., at a distance from the staining platform and the slide support structure); the other means that the slide moving mechanism drives the slide to move in translation along the slide support structure while contacting the slide support structure. The translational movement may effect a change in the relative position of the slide and the staining platform. Typically, the slides are driven to translate without contacting the slide support structure for movement of the slides from one staining zone to another, or from the staining zone to the wash zone, for only aerial transfer, and the stained color can be fixed during movement. And the slide is driven to translate under the condition of contacting with the slide supporting structure, so that the slide can be fully contacted with the staining solution in the translation process, the staining solution is diffused to all areas of the sample on the slide, and the staining quality is improved. The moving mode is particularly suitable for a double dyeing method which needs to use various dyeing liquids for dyeing. The slide moving mechanism moves the slide up, down and horizontally, so that the slide can enter different dyeing areas for dyeing, or enter another dyeing area from one dyeing area for counterdyeing; the sample with short dyeing time can be moved to the sample with long dyeing time for processing, optional dyeing of different types of samples can be realized in the device provided by the invention, the operation is flexible, and resources such as reagents and the like can be saved.
The multifunctional optional dyeing device provided by the invention can be used for delivering different types of samples to different dyeing areas of the dyeing platform for dyeing treatment through the matching of the dyeing platform, the slide supporting structure and the slide moving mechanism, and can also be used for transferring a certain specific slide in a certain dyeing area, so that the samples with different dyeing time or different requirements on the used dyeing solution can be dyed by the same device. Meanwhile, the dyeing area can be set according to the requirements of different types of samples, so that the multifunctional optional dyeing device provided by the invention can meet the dyeing requirements of different samples (such as gynecological samples, blood samples and the like), and can realize selection and movement of optional samples through a slide moving mechanism.
Drawings
In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the drawings needed to be used in the description of the embodiments or the prior art will be briefly described below, it is obvious that the drawings in the following description are only some embodiments described in the present application, and other drawings can be obtained by those skilled in the art without creative efforts.
FIG. 1 is a schematic structural view (circular) of a multifunctional optional dyeing device in an embodiment of the invention;
FIG. 2 is a schematic cross-sectional view (circular) of a multifunctional optional dyeing apparatus in an embodiment of the present invention;
FIG. 3 is a schematic structural view (rectangular) of a multifunctional optional dyeing device in an embodiment of the present invention;
FIG. 4 is a schematic structural diagram of a holding part and a driving part of the multifunctional optional dyeing device in the embodiment of the invention;
reference numerals: 1-a dyeing platform; 2-a slide support structure; 3-a slide moving mechanism; 31-a clamping member; 32-a drive member; 32 a-vertical drive member; 32 b-a translational drive member; 33-slide tray; 34-a rotary drive member; 35-a lifting drive member; a-glass slide; b-a liquid supply hole; c-a liquid supply tank; d-a replenishment tank; e-an overflow tank.
Detailed Description
In order to make those skilled in the art better understand the technical solutions in the present application, the technical solutions in the embodiments of the present application will be clearly and completely described below with reference to the drawings in the embodiments of the present application, and it is obvious that the described embodiments are only a part of the embodiments of the present application, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present application.
Referring to fig. 1 to 4, an embodiment of the invention provides a multifunctional optional staining apparatus, including a staining platform 1, a slide supporting structure 2, a slide moving mechanism 3,
the dyeing platform 1 is provided with at least one cleaning area and at least two dyeing areas, wherein the cleaning area and the dyeing areas are respectively supplied with cleaning liquid and dyeing liquid;
the glass slide supporting structures 2 are arranged on two sides of the staining platform 1 and used for supporting a glass slide to be horizontally placed above the staining platform 1 and enabling the glass slide and the surface of the staining platform 1 to form a gap;
the slide moving mechanism 3 is used for driving the slide to move up or down or in a translational motion, so that the slide moves, and optionally leaves or enters a certain dyeing area or a cleaning area.
The existing staining device can only stain the same specimen in batch, and each slide is stained by each staining area and a cleaning area of the staining device in sequence. In practical application, a plurality of small and medium hospitals have small single sample amount and small batch dyeing requirement, but different samples (such as gynecology, blood and the like) need to be dyed. In the patent previously filed by the applicant, the slide can only be stained by a pipeline mode through each staining area of the staining platform, and different staining areas can not be selected for staining different staining requirements of each sample.
Aiming at the problems in the prior art, the invention provides a multifunctional optional dyeing device which can realize dyeing of different samples in different modes, and different dyeing areas are selected to dye the samples according to the dyeing requirements of the samples. The invention provides a multifunctional optional dyeing device which comprises a dyeing platform 1, a glass slide supporting structure 2 and a glass slide moving mechanism 3, wherein the glass slide supporting structure 2 is arranged on two sides of the dyeing platform 1, supports a glass slide to be horizontally placed above the dyeing platform 1, and enables the glass slide and the surface of the dyeing platform 1 to form a gap. In the present invention, the surface of the staining stage 1 refers to a liquid surface formed by the staining solution or the cleaning solution contained in the staining stage 1, and the liquid surface is located below the slide. Due to the action of the surface tension of the liquid, the dyeing liquid or the cleaning liquid can form a liquid film on the surface of the dyeing platform, and the height of the liquid surface of the liquid film exceeds the height of the glass slide, so that the dyeing liquid or the cleaning liquid in the dyeing platform 1 can be soaked into a sample on the glass slide to dye or clean the sample.
Meanwhile, the dyeing platform 1 is provided with at least two dyeing areas, and with at least one cleaning area, each dyeing area provides different dyeing solutions, and the cleaning area provides cleaning solution to clean redundant dyeing solutions on the slide. The staining platform 1 can be provided with 2-3 staining areas (such as Ruhrstan's staining area and gram staining area), or the number of the staining areas can be flexibly set according to the actual sample staining requirement. Different staining areas can be set to provide different staining solutions to stain different samples, and the slide moving mechanism 3 moves the slides to the corresponding staining areas to stain the samples, so that the technical requirements of optionally staining various types of samples are met. Different dyeing areas are separated from each other through a partition plate, and different working liquids are prevented from influencing each other.
The slide moving mechanism 3 is arranged to drive the slide to move up or down or move in a translation mode so as to move the slide. The ascending motion means that the slide moving mechanism 3 drives the slide to ascend so as to enable the slide to be separated from the staining platform 1 and the slide supporting structure 2 by a certain distance; the descending motion means that the slide moving mechanism 3 drives the slide to descend so as to make the slide contact with the slide supporting structure 2; the translational motion includes two types, one is that the slide moving mechanism 3 drives the slide to translate without contacting the slide support structure 2 (i.e. at a distance from the staining platform 1 and the slide support structure 2); the other means that the slide moving mechanism 3 drives the slide to make a translational motion along the slide support structure 2 while contacting the slide support structure 2. The translational movement may effect a change in the relative position of the slide and the staining platform 1. Typically, the slides are driven to translate without contacting the slide support structure 2 for movement of the slides from one staining zone to another, or from the staining zone to the wash zone, for only aerial transfer, and the stained color can be fixed during movement. And the slide is driven to translate under the condition of contacting the slide supporting structure 2, so that the slide can be fully contacted with the staining solution in the translation process, the staining solution can be diffused to all areas of the sample on the slide, and the staining quality is improved. The moving mode is particularly suitable for a double dyeing method which needs to use various dyeing liquids for dyeing. The slide moving mechanism 3 moves the slide up, down and horizontally to realize that the slide enters different dyeing areas for dyeing or enters another dyeing area from one dyeing area for counterdyeing; the sample with short dyeing time can be moved to the sample with long dyeing time for processing, optional dyeing of different types of samples can be realized in the device provided by the invention, the operation is flexible, and resources such as reagents and the like can be saved.
According to the multifunctional optional dyeing device provided by the invention, through the matching of the dyeing platform 1, the slide supporting structure 2 and the slide moving mechanism 3, different types of samples can be sent to different dyeing areas of the dyeing platform 1 for dyeing treatment, and a specific slide in a certain dyeing area can be transferred, so that the samples with different dyeing time or different requirements on the used dyeing solution can be dyed by the same device. Meanwhile, the dyeing area can be set according to the requirements of different types of samples, so that the multifunctional optional dyeing device provided by the invention can meet the dyeing requirements of different samples (such as gynecological samples, blood samples and the like), and can realize selection and movement of optional samples through the slide moving mechanism 3.
Preferably, the slide moving mechanism 3 includes a gripping member 31 for gripping the slide, and a driving member 32 for driving the gripping member 31 to move.
As one embodiment of the slide moving mechanism 3 of the present invention, the slide moving mechanism 3 may employ a configuration of a gripping member 31 and a driving member 32. The holding part 31 is used for holding and fixing the slide, and the driving part 32 can drive the holding part 31 to perform ascending or descending or translational motion, so as to drive the slide held by the holding part 31, and to realize the position change of the slide on the staining platform 1. Further, the gripping member 31 and the driving member 32 also enable the slide to enter and exit the staining platform 1. The clamping component 31 is driven by the driving component 32 to move, so that the slide to be dyed can be sent into the dyeing platform 1 for dyeing, and the slide after dyeing and cleaning can also be sent out of the dyeing platform 1 and enters a dyed slide storage position or enters a detection device for detection. The driving component 32 can cooperate with the vertical driving component 32a and the translational driving component 32b to realize the ascending, descending and translational movement of the driving component 32, so as to change the position of the slide.
Preferably, the slide moving mechanism 3 includes a slide tray 33, a rotation driving component 34, and a lifting driving component 35, wherein the slide tray 33 is disposed above the staining platform 1 and is used for holding a slide, the rotation driving component 34 drives the slide tray 33 to rotate, and the lifting driving component 35 drives the slide tray 33 to move up and down in the vertical direction.
As another embodiment of the slide moving mechanism 3 of the present invention, the slide moving mechanism 3 may be configured as a slide tray 33, a rotation driving member 34, and an elevation driving member 35, wherein the slide tray 33 is disposed on the staining platform 1 for supporting the slide, and the rotation driving member 34 and the elevation driving member 35 respectively realize rotation, elevation, and descent movements of the slide tray 33, so as to realize translation, elevation, and descent movements of the slide on the slide tray 33. A rotation drive unit 34 may be provided in association with the slide tray and an elevation drive unit 35 may be provided in association with the rotation drive unit 34 to effect translational or elevation and lowering movement of the slide tray 33. In this embodiment, the movement of the slide tray 33 is controlled by the operations of the rotation driving member 34 and the elevation driving member 35, and the position of the slide held by the slide tray is changed.
Generally, the scheme of moving the slide by using the clamping component 31 and the driving component 32 is that the slide does not need to move in the dyeing process, and only enters or leaves a dyeing area and a cleaning area, so that the clamping component 31 and the driving component 32 are required to work, and the scheme is particularly suitable for fixed dyeing. And the scheme of using the slide tray 33, the rotary driving part 34 and the lifting driving part 35 ensures that the slide moves slowly along with the slide tray in the dyeing process, namely in the process of contacting the slide with the dyeing solution, and is particularly suitable for batch dyeing and counterdyeing. Batch dyeing and counterdyeing can be realized by the clamping component 31 and the driving component 32, or fixed dyeing can be realized by the slide tray 33, the rotary driving component 34 and the lifting driving component 35 according to requirements.
Preferably, the slide moving mechanism 3 further includes upper and lower sheet members for moving the slide into and out of the slide tray 33.
The slide moving mechanism 3 is provided with an upper slide component and a lower slide component which are matched with the slide shifting tray 33 for use, and the operation of entering and sending out the slide from the dyeing platform 1 can be realized by shifting the slide into and out of the slide shifting tray 33.
Preferably, at least one slide placing groove is provided on the slide tray 33.
The slide tray 33 is provided with a slide placing groove for better fixing the slide. Meanwhile, when a plurality of glass slide placing grooves are arranged, the glass slides can be placed at intervals through the intervals among the glass slide placing grooves, and different glass slides are placed to be mutually influenced in the dyeing or cleaning process.
Preferably, the slide moving mechanism 3 includes a clamping mechanism, a driving mechanism, a rotating tray, and a rotating driving mechanism, the rotating tray is disposed above the staining platform and used for supporting the slide, the rotating driving mechanism drives the rotating tray to rotate, the clamping mechanism is used for clamping the slide, and the driving mechanism is used for driving the clamping mechanism to move, so that the slide moves, leaves or contacts the rotating tray.
As a third embodiment of the slide moving mechanism 3 of the present invention, a clamping mechanism, a driving mechanism, a rotary tray and a rotary driving mechanism can be used in combination, wherein the rotary tray is used for supporting the slide, and the rotary driving mechanism is used for driving the rotary tray to rotate so as to realize the translation of the slide under the condition of contacting with the rotary tray; and the clamping mechanism can make the clamped slide ascend or descend on the rotary tray under the driving of the driving mechanism so as to realize the ascending and descending movement of the slide or change the position of the slide in the rotary tray, thereby making the slide contact with different dyeing areas or cleaning areas of the dyeing platform 1. In this embodiment, the rotating tray rotates at a fixed position, so that the glass slide supported by the rotating tray is contacted with the staining solution or the cleaning solution; the clamping mechanism is used for placing the slide into a specific position in the rotating tray or taking the slide out of the rotating tray so as to realize the movement of the slide into or out of the staining device or the movement between different staining areas of the staining platform.
In the invention, the staining platform 1, the slide tray 33 and the rotating tray can be arranged in a ring shape, and the staining platform 1 can be matched with the slide tray 33 or the rotating tray. The dyeing platform 1, the slide tray 33 and the rotary tray are arranged in a ring shape, so that all slides on the slide tray 33 can be in contact with the dyeing platform 1; the staining platform 1 may be formed in a linear shape or a curved shape with a curvature, and a part of the slide on the slide tray 33 is brought into contact with the staining platform 1 when the slide tray is rotated.
Preferably, the staining area comprises one or more staining sections, different staining solutions are respectively provided in different staining sections in the same staining area, and the slide moving mechanism 3 drives the slide to move into different staining areas and to sequentially move horizontally into each staining section for staining.
The dyeing device provided by the invention can be used for setting the dyeing area according to different dyeing liquids. Namely, a plurality of staining areas are arranged, and each staining area provides different staining solutions, so that the slide is moved to the staining area corresponding to the first-step staining by using the slide moving mechanism 3 for staining according to different sample staining requirements, and after the first-step staining is finished, the slide is moved to the staining area corresponding to the second-step staining by using the slide moving mechanism 3 for staining. And moving the slide between the dyeing areas until the dyeing is finished, and then sending the slide into the cleaning area for cleaning to obtain the slide which is dyed and cleaned.
The staining device provided by the invention can also be used for setting a staining area according to the type of the sample. That is, one or more staining sections are arranged in a staining area, each staining section is respectively provided with different staining solutions, so that the slide moves horizontally between the staining sections of the staining area and the staining process of the sample is completed. The number of the staining sections in the staining area and the staining solution provided by each staining section are set according to the staining requirements of the sample corresponding to the staining area. The present invention provides a device for separating the staining platform 1 into staining areas (such as blood samples, lymph samples, etc.) of different samples, and using the slide moving mechanism 3 to respectively send the samples into the corresponding staining areas according to the types of the samples, so that the slides attached with the samples sequentially pass through the staining sections in the staining areas for staining. The number of the staining sections in the staining area and the type of the staining solution provided are set according to the requirements of the corresponding sample. When the staining areas are set according to the types of the samples, a cleaning area can be arranged at the tail end of the advancing direction of each staining area, and the stained slide moves horizontally to enter the cleaning area for cleaning; and a uniform cleaning area can be arranged, so that the stained slide in each staining area is respectively moved to the cleaning area by the slide moving mechanism 3 for cleaning.
Preferably, the cleaning area and the dyeing area of the dyeing platform 1, or each dyeing section in the dyeing area, are respectively provided with an independent working liquid output mechanism.
At the washing district and the dyeing district of dyeing platform 1, perhaps when the dyeing district sets up a plurality of dyeing sections, be equipped with independent working liquid output mechanism respectively at each dyeing section, can realize supplying with different dyeing with working liquid (dyeing liquid promptly) to each dyeing district to correspond the dyeing demand of different samples. At the same time, a cleaning working liquid (i.e., a cleaning liquid) is also supplied to the cleaning zone. Each zone is respectively supplied with the same or different (preferably different) dyeing liquid or cleaning liquid, and the independent working liquid output mechanisms of each zone or each section are respectively connected with storage devices of different working liquids to work independently. And a part of dyeing area can be selected to work according to the dyeing requirement of the sample, and the part of dyeing area does not work so as to save reagents and energy.
Preferably, the working liquid output mechanism comprises a liquid supply hole and a liquid supply groove, wherein the liquid supply hole is used for supplying working liquid, two ends of the liquid supply groove extend to the edge of the dyeing platform 1, and the liquid supply hole is arranged in the liquid supply groove.
The working liquid output mechanism comprises a liquid supply hole and a liquid supply tank, the liquid supply hole is communicated with a storage device of working liquid (dyeing liquid or cleaning liquid), a pump or a switch is arranged for controlling, the working liquid flows out from the liquid supply hole and flows through the liquid supply tank until a dyeing area or a cleaning area is filled, and the surface tension of the liquid is utilized to realize the dyeing or cleaning of the sample on the glass slide.
In the invention, the working liquid output mechanism can also be provided with a supplement groove which is communicated with the liquid supply hole and used for supplementing working liquid. Part of the liquid supply holes can be communicated with the liquid supply groove, the other part of the liquid supply holes can be communicated with the supplement groove, and the working liquid can be supplemented through the supplement groove in the using process. Preferably, one end of the replenishment tank is provided with a liquid supply hole (the liquid supply hole is arranged near the center line of the dyeing area), the other end of the replenishment tank reaches the edge of the dyeing area, and different replenishment tanks are in contact with different edges of the dyeing area so as to replenish the working liquid to the dyeing area of the corresponding half area of the replenishment tank.
In the present invention, an overflow groove may be disposed around the dyeing platform 1, and the overflow groove is used for discharging an excessive dyeing liquid or cleaning liquid. The overflow groove is arranged at the periphery of the dyeing platform 1 and used for discharging excessive dyeing liquid or cleaning liquid, so that the height of the inner liquid level of the dyeing area or the cleaning area is suitable for the dyeing or cleaning operation of the specimen on the slide, and the phenomenon that the excessive working liquid overflows to influence the use of the device is avoided. The overflow launders may be arranged on one or both sides of the dyeing platform 1. When the dyeing platform 1 is annular, the overflow groove is also annular and is arranged along the circumferential direction of the dyeing platform 1.
In the invention, a control mechanism for controlling the operation of the device can be further arranged, and the control mechanism is respectively connected with the slide moving mechanism 3 and the working liquid output mechanism. The control mechanism is arranged, so that automatic control of the sample entering, moving and leaving the dyeing area can be realized, and the workload of operators is reduced.
The invention also provides a method of using the apparatus of any one of the above claims, comprising the steps of:
a. a slide moving mechanism 3 is used for moving the slide to the position above a dyeing area needing to enter, then the slide is moved downwards and is flatly placed on the slide supporting structure 2, and a gap is formed between the slide and the surface of the dyeing platform 1;
b. a working liquid output mechanism is used for supplying dyeing liquid and cleaning liquid, and a slide moving mechanism 3 is used for driving a slide to move horizontally on a dyeing platform 1 and is fully contacted with the dyeing liquid;
c. after dyeing is finished, moving the glass slide upwards by using a glass slide moving mechanism 3, then translating the glass slide to move to the position above a cleaning area, moving the glass slide downwards, and flatly placing the glass slide on the glass slide supporting structure 2 to form a gap between the glass slide and the surface of the dyeing platform 1 so as to enable the glass slide to be fully contacted with a cleaning solution;
d. after the cleaning, the slide is taken out by using the slide moving mechanism 3.
In the step, the supply of the staining solution and the cleaning solution using the working liquid delivery mechanism may be performed as follows: the working liquid output structure conveys the staining liquid to a liquid supply groove through a liquid supply hole in the staining area until a gap between the table top of the staining area and the glass slide is filled, and the sample on the glass slide is stained or cleaned; and the redundant dyeing liquid or cleaning solution enters the overflow groove. In the invention, the automation of the operation can be realized by a control mechanism.
The previous description of the disclosed embodiments is provided to enable any person skilled in the art to make or use the present invention. Various modifications to these embodiments will be readily apparent to those skilled in the art, and the generic principles defined herein may be applied to other embodiments without departing from the spirit or scope of the invention. Thus, the present invention is not intended to be limited to the embodiments shown herein but is to be accorded the widest scope consistent with the principles and novel features disclosed herein.
Claims (5)
1. A multifunctional optional staining device is characterized by comprising a staining platform (1), a slide supporting structure (2) and a slide moving mechanism (3),
the dyeing platform (1) is provided with at least one cleaning area and at least two dyeing areas, wherein the cleaning area and the dyeing areas are respectively supplied with cleaning liquid and dyeing liquid;
the dyeing platform (1) is annular, and the glass slide supporting structures (2) are arranged on two sides of the dyeing platform (1) and used for supporting a glass slide to be horizontally placed above the dyeing platform (1) and enabling the glass slide and the surface of the dyeing platform (1) to form a gap;
the slide moving mechanism (3) is used for driving the slide to do ascending, descending and translation motions so as to enable the slide to move and optionally leave and enter a certain dyeing area or a certain cleaning area;
the slide moving mechanism (3) comprises a clamping mechanism, a driving mechanism, a rotating tray and a rotating driving mechanism, wherein the rotating tray is arranged above the dyeing platform and used for bearing the slide, the rotating driving mechanism drives the rotating tray to rotate so as to realize translation of the slide under the condition of contacting the rotating tray, and the translation specifically refers to the translation movement along the slide supporting structure (2) under the condition that the slide contacts the slide supporting structure (2); the clamping mechanism is used for clamping the slide, and the driving mechanism is used for driving the clamping mechanism to move so as to move the slide, so that ascending and descending movement can be realized, and the position of the slide in the rotary tray can be changed, so that the slide is in contact with different dyeing areas or cleaning areas of the dyeing platform (1).
2. The apparatus according to claim 1, wherein the staining zone comprises one or more staining sections, different staining solutions are provided in different staining sections in the same staining zone, and the slide moving mechanism (3) drives the slide to move into different staining zones and to translate into each staining section in turn for staining.
3. The device according to claim 2, characterized in that the washing zone and the dyeing zone of the dyeing platform (1), or each dyeing section in the dyeing zone, are respectively provided with an independent working liquid output mechanism.
4. The device according to claim 3, characterized in that the working liquid output mechanism comprises a liquid supply hole for supplying working liquid and a liquid supply tank, both ends of the liquid supply tank extend to the edge of the dyeing platform (1), and the liquid supply hole is arranged in the liquid supply tank.
5. A method of using the apparatus of any of claims 1-4, comprising the steps of:
a. a slide moving mechanism (3) is used for moving the slide to a position above a dyeing area needing to enter, then the slide is moved downwards and is flatly placed on the slide supporting structure (2), and a gap is formed between the slide and the surface of the dyeing platform (1);
b. a working liquid output mechanism is used for supplying staining solution and cleaning solution, a slide moving mechanism (3) is used for driving a slide to translate on a staining platform (1) and is in full contact with the staining solution;
c. after dyeing is finished, moving the glass slide upwards by using a glass slide moving mechanism (3), then translating, moving the glass slide to the position above the cleaning area, moving the glass slide downwards, flatly placing the glass slide on the glass slide supporting structure (2), forming a gap between the glass slide and the surface of the dyeing platform (1), and enabling the glass slide to be fully contacted with the cleaning solution;
d. after the cleaning, the slide is taken out by using a slide moving mechanism (3).
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| CN201810129669.9A CN108088726B (en) | 2018-02-08 | 2018-02-08 | Multifunctional optional dyeing device |
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| CN201810129669.9A CN108088726B (en) | 2018-02-08 | 2018-02-08 | Multifunctional optional dyeing device |
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| CN108088726B true CN108088726B (en) | 2020-12-22 |
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| CN108535079B (en) * | 2018-05-31 | 2024-08-16 | 广州江元医疗科技有限公司 | Multifunctional slide fixing turntable |
| CN110057649A (en) * | 2019-05-21 | 2019-07-26 | 广西农业职业技术学院 | Use for laboratory slide staining device |
| JP7223648B2 (en) * | 2019-06-26 | 2023-02-16 | シスメックス株式会社 | Smear Preparation Apparatus, Smear Preparation Apparatus Control Method, and Specimen Processing Apparatus |
| CN112823273A (en) * | 2019-06-27 | 2021-05-18 | 深圳迈瑞生物医疗电子股份有限公司 | Wafer pushing machine and control method thereof |
| CN110470524A (en) * | 2019-08-02 | 2019-11-19 | 贵州金域医学检验中心有限公司 | A kind of pathology slide dyeing apparatus and colouring method |
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| FR2426254A1 (en) * | 1978-05-17 | 1979-12-14 | Atlanta Sarl | BLADE STAINING DEVICE |
| EP1511569A1 (en) * | 2002-06-13 | 2005-03-09 | Millenium Biologix AG | Reaction chamber |
| CN101050416B (en) * | 2007-02-02 | 2010-12-01 | 杭州浙大优创科技有限公司 | Automatic counting instrument for body cell of milk / bacteria through direct microscopic method |
| CN201724855U (en) * | 2010-07-06 | 2011-01-26 | 赵辉 | Dyeing bracket used for novel clinical examination |
| US8945662B2 (en) * | 2012-09-06 | 2015-02-03 | Rushabh Instruments, Inc. | Rotary slide stainer |
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| CN106248464B (en) * | 2016-09-02 | 2020-06-09 | 四川大学 | Cell climbing sheet efficient dyeing device |
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Address after: 410000 Aiwei medical science and Technology Park, No.26 Fuling Road, bachelor street, Yuelu District, Changsha City, Hunan Province Patentee after: AVE SCIENCE & TECHNOLOGY Co.,Ltd. Country or region after: China Address before: 410013 B6, 27 Yuan Lu Yuan Yuan, Wen Xuan Road, Changsha hi tech Development Zone, Hunan. Patentee before: AVE SCIENCE & TECHNOLOGY Co.,Ltd. Country or region before: China |