CN108077284A - A kind of Rodolia limbata attractant and application for being used to prevent giant mealy bug - Google Patents
A kind of Rodolia limbata attractant and application for being used to prevent giant mealy bug Download PDFInfo
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- CN108077284A CN108077284A CN201810000523.4A CN201810000523A CN108077284A CN 108077284 A CN108077284 A CN 108077284A CN 201810000523 A CN201810000523 A CN 201810000523A CN 108077284 A CN108077284 A CN 108077284A
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- attractant
- limbata
- rodolia
- mealy bug
- giant mealy
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N43/00—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds
- A01N43/90—Biocides, pest repellants or attractants, or plant growth regulators containing heterocyclic compounds having two or more relevant hetero rings, condensed among themselves or with a common carbocyclic ring system
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01M—CATCHING, TRAPPING OR SCARING OF ANIMALS; APPARATUS FOR THE DESTRUCTION OF NOXIOUS ANIMALS OR NOXIOUS PLANTS
- A01M1/00—Stationary means for catching or killing insects
- A01M1/02—Stationary means for catching or killing insects with devices or substances, e.g. food, pheronones attracting the insects
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N27/00—Biocides, pest repellants or attractants, or plant growth regulators containing hydrocarbons
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N29/00—Biocides, pest repellants or attractants, or plant growth regulators containing halogenated hydrocarbons
- A01N29/02—Acyclic compounds or compounds containing halogen attached to an aliphatic side-chain of a cycloaliphatic ring system
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01M—CATCHING, TRAPPING OR SCARING OF ANIMALS; APPARATUS FOR THE DESTRUCTION OF NOXIOUS ANIMALS OR NOXIOUS PLANTS
- A01M2200/00—Kind of animal
- A01M2200/01—Insects
- A01M2200/012—Flying insects
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- Life Sciences & Earth Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Wood Science & Technology (AREA)
- Environmental Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Dentistry (AREA)
- Agronomy & Crop Science (AREA)
- Plant Pathology (AREA)
- Insects & Arthropods (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The present invention relates to woodland deinsectization technical fields.A kind of Rodolia limbata imago attractant, by the 1R. α firpenes, the 1 chlorine octadecane that density is 10^ 3g/mL, 1 that density is 10^ 2g/mL that density is 10^ 3g/mL, 8 Cineole installation volumes are than 2:1:16 be uniformly mixed i.e. form Rodolia limbata imago attractant.The invention further relates to Rodolia limbata imago attractant woodland application.Rodolia limbata imago attractant of the present invention lures Rodolia limbata effect apparent and nontoxic, and environment will not be polluted.
Description
Technical field
The present invention relates to woodland deinsectization technical fields.
Background technology
Giant mealy bug Drosichacorpulenta belongs to Semiptera Hemiptera, Coccoidea Coccoidea, is that one kind is posted
Main extensive, a variety of forests of harm and the plant-feed insect of fruit tree, have very big danger to northern China willow, persimmon, jujube tree and peach
Evil.It has been observed that East Village (110 ° 54 ' 15 " E, 35 ° 39 ' 55 " N, the m) giant mealy bug of height above sea level 557 are opened in Yuncheng Jishan County
Repeatedly occur on persimmon, willow.Therefore, prevention giant mealy bug is the key link for ensureing the economic fruit-bearing forest good harvest such as persimmon.At present
Domestic and international biological control worker has carried out extensive research to the biological characteristics and its Prevention Technique of giant mealy bug, but still
Based on chemical prevention, however giant mealy bug breeding amount is big, and the secretion of polypide surface has wax protection, and mostly by hard appearance
Skin makes chemical insecticide be extremely difficult to preferable control effect, pollutes fruit and environment instead, health is damaged,
And natural enemy is largely killed, giant mealy bug is made to cause harm more and is difficult to control.Therefore new control of insect measure is studied to ensureing food
It is particularly important with ecological safety.
Rodolia limbata RodolialimbataMotschulsky belongs to coleoptera Coleoptera Coccinellidaes
Coccinellidae is the Natural Enemies ladybug of coccid.Research finds that Rodolia limbata has giant mealy bug on apparent taxis, and red ring
The history of life of ladybug and giant mealy bug is identical, and larva and adult can prey on giant mealy bug, to giant mealy bug control action very
Substantially.
The content of the invention
The technical problems to be solved by the invention are：How coccid to be carried out using chemical information attraction natural enemy ladybug biological
Prevention.
The technical solution adopted in the present invention is：A kind of Rodolia limbata attractant for being used to prevent giant mealy bug, feature exist
In：By 1R. australenes that density is 10^-3g/mL, density be 10^-3g/mL 1- chlorine octadecane, density 10^-2g/mL
1,8- Cineole installation volumes than 2:1:16 be uniformly mixed i.e. form Rodolia limbata imago attractant.
A kind of application for the Rodolia limbata attractant for being used to prevent giant mealy bug, carries out in accordance with the following steps
Step 1: making attractant means, the Rodolia limbata imago attractant of 3ml is added dropwise on absorbent cotton, then by absorbent cotton
Attractant means making is completed in sealing after filling in 10ml Brown Glass Brown glass bottles and jars onlys, and attractant means are stored in -20 DEG C of environment；
Step 2: field traps, in the case where temperature is 27-31 DEG C, relative humidity 50-65%, wind speed are 4-10 m/s,
Attractant means taking-up is hung in the woods there are giant mealy bug harm at 1.5 m from the ground.
As a kind of preferred embodiment：It is needed replacing daily once mounted on woodsy attractant means.
The beneficial effects of the invention are as follows：Rodolia limbata imago attractant of the present invention lures Rodolia limbata effect apparent, and
It is nontoxic, environment will not be polluted.
Specific embodiment
Attractant means are made, the 1- chlorine 18 for being 10^-3g/mL by 1R. australenes that concentration is 10^-3g/mL, concentration
Alkane, the 1,8- Cineole installation volumes that concentration is 10^-2g/mL are than 2:1:16 are uniformly mixed and form Rodolia limbata adult and lure
Agent.The Rodolia limbata imago attractant of 3ml is added dropwise on absorbent cotton, is then filled in absorbent cotton close after 10ml Brown Glass Brown glass bottles and jars onlys
Envelope is completed attractant means and is made, and attractant means is stored in spare in -20 DEG C of environment.
Field traps：Choose persimmon growing way, the giant mealy bug extent of injury and two pieces of experiments similar in the distribution of Rodolia limbata adult
Forest land is respectively as experiment forest land and control forest land, and two forest lands are at a distance of 1 km.In the generation phase of Rodolia limbata, picking is fine
Date attractant means are hung at 1.5 m from the ground.Attractant is replaced daily, after 3 days statistic record is needed each to lure
The Rodolia limbata head number lured on 5 persimmons around agent in 3 m.In the environment of forest land, 27-31 DEG C of temperature, humidity 50-
65%, wind speed 4-10 m/s.Record data.Statistical result carries out difference analysis with t inspections.Draw the persimmon there are attractant
It sets the ladybug average being attracted in three days and has reached 82, and the ladybug average on the persimmon of control group in three days is 23
Head.The pole level of signifiance is reached.Thus illustrate that this inducing agent attracting action is apparent and nontoxic, environment will not be caused
Pollution.And attractant preparation method, it is easy to operate, it is of low cost.Suitable for extensive use.
The attractant that the present invention uses is maximally efficient by what is obtained in the analysis to giant mealy bug polypide volatile substance
Method.（1）Giant mealy bug polypide elutes（2）Preliminary experiment have chosen 65 μm of PDMS/DVB of extracting fiber to two ages of giant mealy bug, three ages,
Adult and ovum are extracted respectively because it detects material abundance, can more, preferably be adsorbed the volatile materials of polypide, are used in combination
GC-MS is analyzed）（3）Components identification：GC conditions：DB-5MS capillary columns（0.25mm×0.25μm×30m）；Temperature programming condition
It is as follows：40 DEG C of initial temperature keeps 5min, then rises to 260 DEG C with 10 DEG C/min, keeps 13min, common 40min.Injector temperature
250 DEG C, carrier gas：He, helium flow velocity 1.0mL/min, not shunting mode.MS conditions：Electron impact ion source（EI）, source temperature
210℃；250 DEG C of transmission line temperature；Electron energy is 70eV, and acquisition range m/z is 45.0-800.0, acquisition mode Scan.Sample
Ingredient in product is qualitative by computer checking and with being obtained after NIST08 standard mass spectrum storehouse matchings, and record wherein matching degree is big
It is quantified in 800 ingredient, and with areas of peak normalization method.（It is shown in Table 1）
1 giant mealy bug of table major volatile constituents and relative amount in different larval instar（%）
Table2The main volatile components and relative content of D. corpulentain different instars（%）
Note："-" expression is not detected.Note："-" indicated undetected
The selection of attractant
（1）Rodolia limbata is tested to seven kinds of one-component substance 1,8- Cineoles, 1- chlorine 18 by indoor and Field tests
Four alkane, diisobutyl phthalate, 2- phenylethanols, 1R. australenes, ethylbenzene and ortho-xylene concentration gradients 10-3 g/
mL、10-4 g/mL、10-5 g/mL、10-6The taxis of g/mL.Draw Rodolia limbata to 10-5The 1,8- Cineoles of g/mL, 10-6
The diisobutyl phthalate of g/mL and 10-6The taxis of the 1R. australenes of g/mL reaches extremely notable water with the taxis compareed
It is flat.To 10-6 The 1- chlorine octadecane of g/mL, 10-4 The taxis of the 2- phenylethanols of g/mL reaches the level of signifiance with the taxis compareed.
（2）Further Rodolia limbata is tested to five kinds of effective one-component substances by indoor and Field tests：10-5
The 1,8- Cineoles of g/mL, 10-6The 1- chlorine octadecane of g/mL, 10-6The diisobutyl phthalate of g/mL, 10-4 g/mL
2- phenylethanols and 10-6The taxis of the two-component reagent of each mixed proportion of the 1R. australenes of g/mL is drawn to 1,8- folium eucalyptis
Element 10-5g/mL:Diisobutyl phthalate 10-6 g/mL(1:1)、10-3The 1- chlorine octadecanes of g/mL: 10-3G/mL's
1R. australenes (1:2)、10-4The 1,8- Cineoles of g/mL: 10-5The diisobutyl phthalate (4 of g/mL:1)、1,
8- Cineoles 10-5g/mL:1R. australenes 10-6g/mL(8:1) taxis of mixed composition reaches extremely significantly with the taxis compareed
It is horizontal.
（3）Choosing has three kinds of proportionings of optimal seduction in binary attractant：10-3The 1,8- Cineoles of g/mL:
10-4The diisobutyl phthalate (1 of g/mL:1)、10-3The 1- chlorine octadecanes of g/mL: 10-3The 1R. australenes of g/mL
(1:And 10 2)-4The 1,8- Cineoles of g/mL: 10-5The diisobutyl phthalate (4 of g/mL:1), based on this may be used
The ternary attractant of 9 group of formula is obtained, 2 the results are shown in Table to luring for Rodolia limbata in woodland.
Woodland is tested（1）Make attractant means：By 10-3G/mL 1R. australenes；10-3G/mL 1- chlorine octadecanes；10-2
G/mL 1,8- Cineoles are according to 2:1:16 be made volume be 3ml attractant, be added dropwise after mixing on absorbent cotton, fill in
It is sealed rapidly after 10ml Brown Glass Brown glass bottles and jars onlys, -20 DEG C save backup.
（2）Field traps：Choose persimmon growing way, the giant mealy bug extent of injury and two pieces of examinations similar in the distribution of Rodolia limbata adult
Forest land is tested respectively as experiment forest land and control forest land, two forest lands are at a distance of 1 km.In the generation phase of Rodolia limbata, picking weather is fine
The bright date hangs on attractant means at 1.5 m from the ground.Attractant is replaced daily, after 3 days statistic record is needed each to draw
The Rodolia limbata head number lured on 5 persimmons around lure in 3 m.In the environment of forest land, 27-31 DEG C of temperature, humidity 50-
65%, wind speed 4-10 m/s.Record data.Statistical result carries out difference analysis with t inspections.
Indoor test：Rodolia limbata tests the taxis of compound.Per the control of arm pipe gas flow in 200 mL min-1.
Ventilate to Y-piece before experiment 10 min, introduces ladybug by head, 20 min of meticulous inspection records behavior reaction and ladybug number.It will climb
To or beyond at a certain pipe arm 1/3 and stop more than 1min or climb out of being denoted as pipe arm and have taxis to the odor source, such as counting
It does not make a choice in time, is denoted as no taxis reaction.25-26 DEG C of room temperature, humidity 31-47%, 10：00-15：00 is tried
It tests.And maximally efficient ingredient extremely concentration is drawn.
The artificial attractant of 2 ternary of table is to the trapping in forest effect of Rodolia limbata
Table 2The attractant effect of ternary artificial attractants toR.limbatain the field
Note：Data are average ± standard error in table.* represents that taxis difference is extremely notable, and * represents taxis significant difference, N.S. tables
Show taxis without significant difference.Note：Data are mean ± SE.** meansP0.01 ＜ of ＜ 0.01, * meansP＜
0.05, N.S. means P＞ 0.05.
By the seduction of 2,9 groups of attractants of analytical table, wherein the attractant effect for having four groups of attractants reaches extremely aobvious with compareing
Level is write, the attractant effect and control group for there are five groups of attractants reach the level of signifiance.Wherein the 8th group of attractant effect is optimal, 3 days
The ladybug average being inside attracted to reaches 82.Thus illustrate that this inducing agent attracting action is apparent and nontoxic, to environment
It will not pollute.And attractant preparation method, it is easy to operate, it is of low cost, suitable for extensive use.
Claims (3)
1. a kind of Rodolia limbata attractant for being used to prevent giant mealy bug, it is characterised in that：By concentration be 10^-3g/mL 1R. α-
The 1,8- Cineole installation volumes that 1- chlorine octadecane that firpene, concentration are 10^-3g/mL, concentration are 10^-2g/mL are than 2:1:
16 be uniformly mixed i.e. form Rodolia limbata imago attractant.
2. a kind of application of the Rodolia limbata attractant described in claim 1 for being used to prevent giant mealy bug, it is characterised in that：According to
Following steps carry out
Step 1: making attractant means, the Rodolia limbata imago attractant of 3ml is added dropwise on absorbent cotton, then by absorbent cotton
Attractant means making is completed in sealing after filling in 10ml Brown Glass Brown glass bottles and jars onlys, and attractant means are stored in -20 DEG C of environment；
Step 2: field traps, in the case where temperature is 27-31 DEG C, relative humidity 50-65%, wind speed are 4-10 m/s,
Attractant means taking-up is hung in the woods there are giant mealy bug harm at 1.5 m from the ground.
3. a kind of application of Rodolia limbata attractant for being used to prevent giant mealy bug according to claim 2, it is characterised in that：
It is needed replacing daily once mounted on woodsy attractant means.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201810000523.4A CN108077284A (en) | 2018-01-02 | 2018-01-02 | A kind of Rodolia limbata attractant and application for being used to prevent giant mealy bug |
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| CN201810000523.4A CN108077284A (en) | 2018-01-02 | 2018-01-02 | A kind of Rodolia limbata attractant and application for being used to prevent giant mealy bug |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2020078484A3 (en) * | 2018-10-17 | 2020-06-11 | 浙江大学 | Natural enemy ladybug attractant for controlling aphids |
Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105746585A (en) * | 2016-05-09 | 2016-07-13 | 福建省农业科学院植物保护研究所 | Attractant for aphid predatory natural enemy insects |
-
2018
- 2018-01-02 CN CN201810000523.4A patent/CN108077284A/en active Pending
Patent Citations (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105746585A (en) * | 2016-05-09 | 2016-07-13 | 福建省农业科学院植物保护研究所 | Attractant for aphid predatory natural enemy insects |
Non-Patent Citations (2)
| Title |
|---|
| 张毅 等: "红环瓢虫对草履蚧及相关气味的趋性", 《安徽农业科学》 * |
| 李伟: "蚧虫虫体挥发物对天敌瓢虫的引诱效应", 《中国优秀硕士学位论文全文数据库 农业科技辑》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2020078484A3 (en) * | 2018-10-17 | 2020-06-11 | 浙江大学 | Natural enemy ladybug attractant for controlling aphids |
| US10959427B2 (en) | 2018-10-17 | 2021-03-30 | Zhejiang University | Attractant of ladybugs, aphid's natural enemy |
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