CN108076254B - Imaging device for colorimetric array sensor - Google Patents
Imaging device for colorimetric array sensor Download PDFInfo
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- CN108076254B CN108076254B CN201610994032.7A CN201610994032A CN108076254B CN 108076254 B CN108076254 B CN 108076254B CN 201610994032 A CN201610994032 A CN 201610994032A CN 108076254 B CN108076254 B CN 108076254B
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- H04N23/00—Cameras or camera modules comprising electronic image sensors; Control thereof
- H04N23/50—Constructional details
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- H—ELECTRICITY
- H04—ELECTRIC COMMUNICATION TECHNIQUE
- H04N—PICTORIAL COMMUNICATION, e.g. TELEVISION
- H04N23/00—Cameras or camera modules comprising electronic image sensors; Control thereof
- H04N23/50—Constructional details
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- H—ELECTRICITY
- H04—ELECTRIC COMMUNICATION TECHNIQUE
- H04N—PICTORIAL COMMUNICATION, e.g. TELEVISION
- H04N23/00—Cameras or camera modules comprising electronic image sensors; Control thereof
- H04N23/50—Constructional details
- H04N23/55—Optical parts specially adapted for electronic image sensors; Mounting thereof
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- H—ELECTRICITY
- H04—ELECTRIC COMMUNICATION TECHNIQUE
- H04N—PICTORIAL COMMUNICATION, e.g. TELEVISION
- H04N23/00—Cameras or camera modules comprising electronic image sensors; Control thereof
- H04N23/56—Cameras or camera modules comprising electronic image sensors; Control thereof provided with illuminating means
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Abstract
The invention relates to an imaging device for a colorimetric array sensor, and belongs to the technical field of analytical instruments. The imaging device comprises a micro CMOS camera, a top plate, a lens barrel, a macro lens, a wide-angle lens, a screw, a guide rod, a screw clamping sleeve, a shell, a colorimetric array sensor, an intermediate plate, a white LED light supplementing plate and a bottom plate. The imaging device realizes the close-range and high-resolution imaging of the contrast color array sensor by reasonably selecting and matching the micro CMOS camera, the macro lens and the wide-angle lens, designing the white LED area light source light supplementing plate and reasonably adjusting each focal length on a mechanical structure, and can completely replace a large-size scanner to capture images of the contrast color array sensor. The device has the advantages of high resolution, small volume, low cost, simple and convenient use, convenient carrying and the like, thereby being particularly suitable for the field rapid detector based on the colorimetric principle and other occasions which take short distance as the first place and have higher resolution requirement.
Description
Technical Field
The invention belongs to the technical field of analytical instruments, and particularly relates to an imaging device for a colorimetric array sensor.
Background
The demand for rapid, sensitive, high-flux and miniaturized detection instruments generally exists in the fields of public safety, environmental monitoring, medical detection and the like. The array sensor based on array analysis adopts a sensing mode of an artificial olfactory system, so that the simultaneous acquisition of multi-point information on a plane can be realized, the analysis efficiency and the detection flux are greatly improved, and a new opportunity is provided for the development of a miniaturized instrument. Among them, the photochemical colorimetric array sensor is increasingly widely used due to its advantages of low price, simple method, fast response, large information amount, etc. The optical signals of all the sensing units in the photo-chemical colorimetric array sensor are generally collected simultaneously by two modes of spectrum or imaging. Although the spectrum technology contains a large amount of information, the spectrum technology needs relatively complex instrument equipment, and the collection of data of each sensing unit also needs a certain time, so the spectrum technology is not suitable for related miniaturized instruments. Compared to spectroscopic techniques, imaging techniques have the advantage that a plurality of sensor units can be detected within a given field of view, and imaging techniques are simpler than spectroscopic techniques and do not require particularly complex instrumentation.
The imaging devices generally used in the photochemical colorimetric array sensor mainly include a flat-panel scanner, a digital camera, a mobile phone camera, and the like. Although the flat-panel scanner has a good imaging effect, the flat-panel scanner has a large volume, and the miniaturization design of related instruments is greatly limited; although the imaging of the contrast color array sensor by using a digital camera, a mobile phone camera and the like is more convenient, the interference of the focal length, the shooting angle, the external light intensity and the like is easily caused, and the stability and the repeatability of the obtained result are poor.
Therefore, how to provide an imaging device for a photochemical colorimetric array sensor, which satisfies the requirements of high resolution, small size, low cost, easy use and portability, is a technical problem to be solved by those skilled in the art.
Disclosure of Invention
In order to solve the above problems, it is an object of the present invention to provide an imaging device for a colorimetric array sensor that can sufficiently satisfy various requirements of a related miniaturized instrument based on the colorimetric principle.
The technical scheme of the invention is as follows:
an imaging device for a colorimetric array sensor: the device comprises a micro CMOS camera, a top plate, a lens barrel, a macro lens, a wide-angle lens, a screw, a guide rod, a screw clamping sleeve, a shell, a colorimetric array sensor, an intermediate plate, a white LED light supplementing plate and a bottom plate.
1) A hollow shell with upper and lower openings; arranged at the lower part
2) A middle plate provided with a colorimetric array sensor; arranged at the lower part
3) The bottom plate is provided with a white LED light supplementing plate;
the shell is mainly composed of a top plate and a lens barrel, and the lens barrel is arranged below the top plate; the top plate is provided with a window, and a micro CMOS camera is arranged at the window; a macro lens and a wide-angle lens are arranged in the lens barrel.
The vertical at least two bellied guide bars (preferably two guide bar symmetries set up) that are equipped with of casing internal face, roof lateral wall face corresponds the position and is equipped with the recess, the recess is equivalent with the guide bar size, and the guide bar is used for making roof and a camera lens section of thick bamboo to keep perpendicular when reciprocating in the casing, and the spiral shell has the detachable screw rod cutting ferrule on the internal face of casing upper portion, and the screw rod cutting ferrule is located on the roof, and threaded through-hole is opened to screw rod cutting ferrule and roof radial relative position, and the screw rod passes through screw rod cutting ferrule and the screw thread through-hole rotary motion on the roof and drives roof and a camera lens section.
The position of the macro lens in the lens barrel is close to one side of the top plate, the position of the wide-angle lens is far away from one side of the top plate, and the macro lens and the wide-angle lens are arranged in parallel relatively.
The guide rod is detachable and fixed on the side wall of the shell through a screw.
The middle of the middle plate is hollowed, and a colorimetric array sensor is placed in the middle of the middle plate, wherein the colorimetric array sensor comprises a transparent reaction kit and a colorimetric array sensor film, the colorimetric array sensor film is placed in the reaction kit, and an indicator capable of reacting with an analyte to be detected is fixedly carried on the colorimetric array sensor film;
the side wall of the middle plate is transversely provided with two through holes, and an inlet pipe and an outlet pipe for introducing an analyte to be detected are inserted;
the bottom plate is provided with a groove which is equal to the middle of the middle plate in size and same in position, and the white LED light supplementing plate is arranged in the groove of the bottom plate.
The middle plate and the bottom plate are fixedly connected with the shell through screws;
the white LED light supplementing plate is made of a plurality of LED lamp beads or LED surface light sources.
The reaction kit is provided with a handle, the colorimetric array sensor membrane is made of one of polyvinylidene fluoride membrane, polyvinyl chloride membrane, polyester film, polycarbonate, cellulose acetate membrane and filter paper, and the indicator is immobilized on the colorimetric array sensor membrane by adopting a polymer or sol-gel method. The indicator immobilized on the colorimetric array sensor film can adopt a polymer or sol-gel method (see Chinese patent: 102466638B), and the analyte to be detected refers to an analyte which can perform a color reaction with the indicator.
The white LED light supplement board softens light emitted by the LEDs evenly and provides visible light supplement illumination for the colorimetric array sensor, and the micro CMOS camera collects images for the colorimetric array sensor after zooming through the macro lens and the wide-angle lens.
The magnification of the macro lens is 1: 1-1: 10, and the wide angle of the wide-angle lens is 0.2-0.7.
Providing a scheme, wherein the length of the screw is 20-60 mm; the casing height is 30mm ~80 mm, and the distance between miniature CMOS camera and the macro lens is 0~ 5mm (the camera is arranged in the roof window, can with the camera setting with the roof bottom keep a plane on, make the distance between camera and the macro lens be 0), distance 0~10 mm between macro lens and the wide angle camera, distance 0~50 mm between wide angle camera and the colorimetric array sensor.
The distances between the lenses are adjusted according to actual needs.
The colorimetric array sensor film refers to a sensor array composed of a plurality of indicators.
The imaging device of the present invention is not limited to a square body, and may be a cylindrical shape or other shapes.
The camera of the imaging device can be connected with a microcomputer through a flat cable, the microcomputer sends an instruction to control the image taking time and record an image, and finally the image is displayed through a display screen connected to the microcomputer.
The invention has the beneficial effects that:
according to the invention, through reasonable selection and matching among the micro CMOS camera, the macro lens and the wide-angle lens, the design of the white LED area light source light supplementing plate and reasonable adjustment of each focal length on a mechanical structure, close-range and high-resolution imaging of the colorimetric array sensor is realized, the micro CMOS camera, the macro lens and the wide-angle lens are not interfered by the outside, the stability and the repeatability are good, and the micro CMOS camera can completely replace a scanner with a large volume to be used as an imaging device of the colorimetric array sensor. The invention has the advantages of high resolution, small volume, low cost, simple and convenient use and convenient carrying, and is particularly suitable for a field rapid detector based on the colorimetric principle and other occasions which take short distance as the first place and have higher resolution requirement.
Drawings
Fig. 1 is a schematic structural diagram of an imaging device for a colorimetric array sensor, in which 1 is a micro CMOS camera, 2 is a top plate, 3 is a lens barrel, 4 is a macro lens, 5 is a wide-angle lens, 6 is a screw, 7 is a guide rod, 8 is a screw ferrule, 9 is a housing, 10 is the colorimetric array sensor, 11 is a middle plate, 12 is a white LED light compensation plate, and 13 is a bottom plate.
Fig. 2 is a graph of a scanner in contrast to an imaging device of the present application.
FIG. 3 is a schematic view of a 100ppm SO chamber using the image forming apparatus of FIG. 12And (3) taking images of the gas methyl red colorimetric sensor every other minute, and extracting the RGB values of the images by using Photoshop software.
FIG. 4 is a schematic view of SO inlet with different concentrations using the image forming apparatus of FIG. 12Taking color by a gaseous bromocresol purple colorimetric sensor, extracting RGB value of an image by using Photoshop software, and obtaining the SO value2Standard curve of detection.
Fig. 5 is a standard curve for ziram detection, which is obtained by using the imaging device of fig. 1 to detect ziram at different concentrations and extracting RGB values of an image by using Photoshop software.
Detailed Description
The invention will be described in detail with reference to the drawings and examples, which are illustrative of the invention and are not to be construed as limiting the invention.
Example 1
As shown in fig. 1, the structural schematic diagram of an imaging device for a colorimetric array sensor is shown, the imaging device for the colorimetric array sensor is composed of a micro CMOS camera 1, a top plate 2, a lens barrel 3, a macro lens 4, a wide-angle lens 5, a screw 6, a guide rod 7, a screw clamp sleeve 8, a housing 9, a colorimetric array sensor 10, a middle plate 11, a white LED light supplement plate 12 and a bottom plate 13.
A hollow case 9 having upper and lower openings; a middle plate 11 of the colorimetric array sensor 10 is arranged below the sensor; a bottom plate 13 provided with a white LED light supplement plate 12 below;
the inside of the shell 9 mainly comprises a top plate 2 and a lens barrel 3, and the lens barrel 3 is arranged below the top plate 2; the top plate 2 is provided with a window, and a micro CMOS camera 1 is arranged at the window; the lens barrel 3 is internally provided with a macro lens 4 and a wide-angle lens 5.
The vertical symmetry of casing 9 internal face is equipped with two bellied guide bar 7, and 2 lateral wall faces of roof correspond the position and are equipped with the recess, the recess is equivalent with guide bar 7 size, and guide bar 7 is used for making roof 2 and lens barrel 3 keep perpendicular when reciprocating in casing 9, and the spiral shell has detachable screw rod cutting ferrule 8 on the internal face of casing 9 upper portion, and screw rod cutting ferrule 8 is located on the roof, and threaded through-hole is opened to radial relative position of screw rod cutting ferrule 8 and roof 2, and the screw rod passes through screw rod cutting ferrule 8 and the screw through-hole rotary motion on the roof 2 and drives roof 2 and lens barrel 3 and reciprocate in casing 9, lens barrel 3 is fixed with roof 2 through the screw.
The macro lens 4 in the lens barrel 3 is positioned at one side close to the top plate 2, the wide-angle lens 5 is positioned at one side far away from the top plate 2, and the macro lens 4 and the wide-angle lens 5 are arranged in parallel relatively.
The guide rod 7 is detachable and fixed on the side wall of the shell 9 through a screw.
The colorimetric array sensor 10 comprises a transparent reaction kit with a handle and a colorimetric array sensor film, the colorimetric array sensor film is arranged in the reaction kit, an indicator capable of reacting with an analyte to be detected is fixedly loaded on the film, the reaction kit is provided with the handle, the colorimetric array sensor film is made of one of polyvinylidene fluoride film, polyvinyl chloride film, polyester film, polycarbonate, cellulose acetate film and filter paper, and the indicator is fixedly loaded on the colorimetric array sensor film by adopting a polymer or sol-gel method.
The side wall of the middle plate 11 is transversely provided with two through holes for inserting an inlet pipe and an outlet pipe of an analyte to be detected;
the bottom plate 13 is provided with a groove with the same size and position as the middle of the middle plate 11, and the white LED light supplement plate 12 is arranged in the groove of the bottom plate 13.
The middle plate 11 and the bottom plate 13 are fixedly connected with the shell 9 through screws;
the white LED light supplement plate 12 is made of an LED surface light source.
The white LED light supplement board 12 softens light emitted by the LEDs uniformly and provides visible light supplement illumination for the colorimetric array sensor 10, and the micro CMOS camera 1 collects images for the colorimetric array sensor 10 after zooming through the macro lens 4 and the wide-angle lens 5.
The magnification of the macro lens 4 is 1:5, and the wide angle of the wide-angle lens 5 is 0.65.
The length of the screw 6 is 40 mm.
The height of the housing 9 is 50 mm.
The distances between the micro CMOS camera 1, the macro lens 4, the wide-angle lens 5 and the colorimetric array sensor 10 are determined by the imaging effect of the colorimetric array sensor, and the principle is to ensure that the imaging of the colorimetric array sensor is clear and has no deformation and the sensors can be completely displayed, for example, when the distance between the wide-angle lens and the colorimetric array sensor is 10mm, the distance between the corresponding macro lens and the wide-angle lens is 5mm, and when the distance between the macro lens and the wide-angle lens is 0mm, the imaging effect is optimal; and when the distance between the wide-angle lens and the colorimetric array sensor is 5mm, the distance between the corresponding macro lens and the wide-angle lens is 10mm, and the distance between the corresponding macro lens and the wide-angle lens is 2mm, the imaging effect is optimal.
Application example 1
A 6x6 prepared sensor array consisting of 36 indicators was taken and imaged by a scanner and the imaging device described in example 1, respectively, and the image is shown in fig. 2. as can be seen from fig. 2, the imaging device described in example 1 is the same as the image obtained by the scanner, which illustrates that the imaging device can be used for colorimetric sensor imaging as a complete replacement for the scanner.
The camera of the imaging device is connected with a Raspberry sending computer Raspberry Pi through a flat cable, the Raspberry sending computer sends an instruction to control the image taking time and record an image, and finally the image is displayed through a 7inch HDMILCD (B) display screen connected to the Raspberry sending computer.
Application example 2
Weighing 4mg of indicator methyl red, putting the indicator methyl red into a 1.5mL centrifuge tube, adding 1mL of sol-gel solution into the centrifuge tube (the preparation method of the sol-gel is that tetraethoxysilane, n-octane triethoxy silane, ethylene glycol methyl ether, propylene glycol methyl ether acetate, acetonitrile, 0.1M hydrochloric acid, Tween 20 and water are mixed according to the volume ratio of 0.5: 0.6: 3.5: 1.4: 0.45: 0.35: 0.03: 1 to obtain 10mL of solution, hydrolyzing the solution at normal temperature for 16 hours to obtain the sol-gel solution), adding 30 microliter of hexamethylenetetramine with the concentration of 0.2M into the sol-gel solution after ultrasonic dissolution, shaking and uniformly mixing the solution, sucking 2 microliter of the indicator solution by a pipette gun, coating the indicator solution on a 0.45 micrometer polyvinyl chloride film, putting the sol-gel solution into a light-proof closed container after the organic solvent is completely volatilized, and storing the sol-gel solution at room temperature and. Cutting the indicator-loaded film into a size corresponding to the size of the bottom of a small transparent box (the material of the small transparent box can be plastic), placing the small transparent box at the bottom of the small box, covering, and heat-sealing the four edges of the small transparent box and the box body to obtain the prepared SO2A gas sensor. Placing the prepared sensor in an imaging device, connecting to a gas pipeline through an analyte inlet pipe of the middle plate 11, and introducing SO with flow rate of 300mL/min, relative humidity of 30% and concentration of 100ppm respectively2Taking images for 10min by using the imaging device in the embodiment 1 in the process, taking images every minute, connecting a camera of the imaging device with a Raspberry-type computer Raspberry Pi through a flat cable, sending an instruction by the Raspberry-type computer to control the image taking time and record images, displaying the images through a 7inch HDMI LCD (B) display screen connected to the Raspberry-type computer, and taking colors of the obtained images by using Photoshop software to obtain SO-associated images2Introducing gas into the sensor, and subtracting the initial RGB value of the indicator color from the RGB value to obtain color change values delta R, delta G and delta B of the indicator, and using a formula
The change of the indicator color value with time was calculated and the results are shown in FIG. 3, which shows that the imaging device can be used to obtain the change with SO well2Gas (es)The color change of the input indicator.
Application example 3
Weighing 2mg of indicator bromocresol purple, putting the indicator bromocresol purple into a 1.5mL centrifuge tube, adding 1mL of sol-gel solution (the preparation method of the sol-gel is that octane triethoxysilane (3-mercaptopropyl) trimethoxy silane: ethylene glycol methyl ether: propylene glycol methyl ether acetate: dichloroethane: 0.1M hydrochloric acid: hexadecyl trimethyl ammonium bromide: water is mixed according to the volume ratio of 0.5: 0.6: 3.5: 1.4: 0.45: 0.35: 0.03: 1 to obtain 10mL of solution, hydrolyzing for 24 hours at normal temperature to obtain sol-gel solution)), adding 20 microliters of potassium hydroxide with the concentration of 0.1M after ultrasonic dissolution, shaking and uniformly mixing, sucking 2 microliters of the indicator solution by a liquid transfer gun, dripping and coating the indicator solution on a whatman No. 41 filter paper, putting the filter paper into a light-proof sealed container after the organic solvent is completely volatilized, and storing the filter paper in a dark place at room temperature under the protection of nitrogen environment for later use. Taking the filter paper fixedly carrying the indicator, cutting the filter paper into a size which is equivalent to the size of the bottom of the small transparent box (the material of the small transparent box can be plastic), placing the filter paper at the bottom of the small transparent box, covering the filter paper, and sealing four edges of the filter paper and the box body by heat to obtain the prepared sensor. Placing the prepared sensor in an imaging device, connecting to a gas pipeline through an analyte inlet pipe to be detected of the middle plate 11, and introducing SO with the flow rate of 300mL/min, the humidity of 30% and the concentration of 12.5, 18.75, 25, 32.5 and 38.75ppm respectively2Gas was allowed to flow for 3min, and SO was introduced into the chamber using the image forming apparatus described in example 12The sensor before and after the gas is used for color taking, a camera of the imaging device can be connected with a Raspberry-sending computer Raspberry Pi through a flat cable, the Raspberry-sending computer can send an instruction to control the image taking time and record an image, the image is displayed through a 7inchHDMI LCD (B) display screen connected to the Raspberry-sending computer, the obtained image is subjected to color taking through Photoshop software to obtain RGB values of the color of an indicator in the sensor, and the initial RGB value of the color of the indicator and the input SO are used for obtaining the RGB values of the color of the indicator in the sensor2Then, the color change values Δ R, Δ G and Δ B of the indicator are obtained by subtracting the color change values Δ R, Δ G and Δ B from the color change values Δ R, Δ G and Δ B of the indicator
Is calculated toUntil different concentrations of SO are introduced2The color change of the indicator after the gas, where ED is the Euclidean distance (Edlidimeric), results are shown in FIG. 4, which shows that the indicator methyl red and bromocresol purple mixture has a concentration of between 12.5 and 38.75ppm SO2The gas has a very good linear relationship, where R20.998, and the linear equation is y 1.2813x-0.2337, where R2For fitting coefficients, x represents SO2Y represents the concentration of (C) with respect to the introduction of SO2The color of the sensor before and after the gas is taken, the difference is subtracted to obtain delta R, delta G and delta B, and then the formula is used
The resulting value is calculated. As can be seen from the above experimental results, the imaging apparatus can be used for colorimetric sensor-based detection of toxic gases.
Application example 4
Weighing 2mg of 1-pyridylazo-2-naphthol into 0.5mL of silica gel sol gel A (tetramethoxysilane: ethylene glycol methyl ether: propylene glycol methyl ether acetate: tetrahydrofuran: 0.05M sulfuric acid: Tween 20: water, mixing in a volume ratio of 1: 3: 2: 0.5: 0.8: 0.03: 0.6, hydrolyzing at 50 ℃ for 8 hours to obtain a silica gel sol gel solution A), carrying out ultrasonic dissolution, sucking 1 mu L of the solution by using a liquid transfer gun, dripping the solution on porous filter paper, and drying at room temperature under the protection of nitrogen to prepare the heavy metal-containing pesticide sensor. Taking a prepared sensor, dropwise adding 400 mu L of zinc thiram aqueous solution with the concentration of 0.5,1,1.5,2,2.5ppm to the sensor, after the solution is sucked to be dry by absorbent filter paper below the sensor, recording images by using the imaging equipment described in embodiment 1, connecting a camera of the imaging device with a Raspberry-serving computer Raspberry Pi through a flat cable, enabling the Raspberry-serving computer to send instructions to control the image-taking time and record the images, finally displaying the images through an HDMI LCD (B) display screen with the model of 7inch connected to the Raspberry-serving computer, taking the sensor enriched with 400 mu L of pure water as an image before reaction, taking the sensor enriched with the zinc thiram solution with each concentration as an image after reaction, digitizing the images before and after reaction by using Photoshop software, extracting Red Green Blue (RGB) channel values, and digitizing the Red Green Blue (RGB) channel values before and after the reactionThe color values of the channels are subtracted to obtain color change values delta R, delta G and delta B of each channel, and then a formula is utilized
The color change value ED before and after the sensor reacts with the ziram solution of each concentration is calculated and shown in FIG. 5. The results show that as the concentration of ziram increases, the sensor color change increases. Plotting the color change value ED and the concentration of the ziram to obtain a standard curve of the heavy metal-containing pesticide sensor for detecting the ziram pesticide, wherein y is 28.79x +15.01 (R)20.989), it can be seen that there is a good linear relationship between indicator color change and ziram concentration in the concentration range of 0.5 to 2.5ppm, indicating that the imaging device can be used in colorimetry for determining pesticides.
Claims (9)
1. An imaging device for a colorimetric array sensor, comprising: the method comprises the following steps:
1) a hollow case (9) having an upper and lower opening; arranged at the lower part
2) A middle plate (11) provided with a colorimetric array sensor (10); arranged at the lower part
3) A bottom plate (13) provided with a white LED light supplementing plate (12);
the lens barrel (3) is arranged below the top plate (2); the top plate (2) is provided with a window, and a micro CMOS camera (1) is arranged at the window; a macro lens (4) and a wide-angle lens (5) are arranged in the lens barrel (3).
The device comprises a hollow shell (9) with an upper opening and a lower opening, a middle plate (11) provided with a colorimetric array sensor (10) and a bottom plate (13) provided with a white LED light supplementing plate (12) from top to bottom in sequence;
the inner wall surface of a shell (9) is vertically provided with at least two raised guide rods (7), the corresponding positions of the side wall surface of a top plate (2) are provided with grooves, the grooves are equal to the guide rods (7) in size, the guide rods (7) are used for enabling the top plate (2) and a lens barrel (3) to keep vertical when moving up and down in the shell (9), the inner wall surface of the upper portion of the shell (9) is screwed with a detachable screw clamping sleeve (8), the screw clamping sleeve (8) is located on the top plate, threaded through holes are formed in the radial opposite positions of the screw clamping sleeve (8) and the top plate (2), the screw drives the top plate (2) and the lens barrel (3) to move up and down in the shell (9) through the threaded through hole rotary motion on the screw clamping sleeve (8) and the top plate (2), and the lens.
2. The imaging apparatus according to claim 1, characterized in that: the position of macro lens (4) is near roof (2) one side in lens barrel (3), and the position of wide-angle lens (5) is far away roof (2) one side, macro lens (4) and wide-angle lens (5) are placed in parallel relatively.
3. The imaging apparatus according to claim 1, characterized in that:
the guide rod (7) is detachable and fixed on the side wall of the shell (9) through a screw.
4. The imaging apparatus according to claim 1, characterized in that:
the middle of the middle plate (11) is hollowed, a colorimetric array sensor (10) is placed, the colorimetric array sensor (10) comprises a transparent reaction kit and a colorimetric array sensor film, the colorimetric array sensor film is placed in the reaction kit, and an indicator capable of reacting with an analyte to be detected is fixedly loaded on the film;
the side wall of the middle plate (11) is transversely provided with two through holes, and an inlet pipe and an outlet pipe for introducing analytes to be detected are inserted;
the bottom plate (13) is provided with a groove which is equal to the middle of the middle plate (11) in size and same in position, and the white LED light supplementing plate (12) is arranged in the groove of the bottom plate (13).
5. The imaging apparatus according to claim 1 or 4, characterized in that:
the middle plate (11) and the bottom plate (13) are fixedly connected with the shell (9) through screws;
the white LED light supplementing plate (12) is made of a plurality of LED lamp beads or LED surface light sources.
6. The imaging apparatus according to claim 4, characterized in that:
the reaction kit is provided with a handle, the colorimetric array sensor membrane is made of one of polyvinylidene fluoride membrane, polyvinyl chloride membrane, polyester film, polycarbonate, cellulose acetate membrane and filter paper, and the indicator is immobilized on the colorimetric array sensor membrane by adopting a polymer or sol-gel method.
7. The imaging apparatus according to claim 1, characterized in that: the white LED light supplementing plate (12) is used for softening light emitted by the LEDs uniformly and then providing visible light supplementing illumination for the colorimetric array sensor (10), and the micro CMOS camera (1) collects images for the colorimetric array sensor (10) after zooming through the macro lens (4) and the wide-angle lens (5).
8. The imaging apparatus according to claim 1 or 6, characterized in that: the magnification of the macro lens (4) is 1: 1-1: 10, and the wide angle of the wide-angle lens (5) is 0.2-0.7.
9. The imaging apparatus according to claim 4, characterized in that: the length of the screw (6) is 20 mm-60 mm; casing (9) height is 30mm ~80 mm, and the distance between miniature CMOS camera and the macro lens is 0~ 5mm, and the distance between macro lens and the wide-angle lens is 0~10 mm, and the distance between wide-angle lens and the colorimetric array sensor is 0~50 mm.
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