A kind of method for detecting interstitialcellstimulating hormone (ICSH) concentration in women body
Technical field
The present invention relates to a kind of conveying of biological sample and detecting system, including take up the examination of biological sample such as blood, urine
The conveying device of pipe and the follow-up detection terminal for carrying out biology sample detection, more particularly to a kind of urine test tube automatic transport dress
Put, belong to B65G fields.
Background technology
In the package packing machine industry such as food, pharmacy, various medicine container containings, for example:Cillin bottle, ampoule
Bottle, oral liquid bottle, infusion bottle etc. have obtained universal utilization.In above-mentioned bottle, most bottleneck will than the diameter of body
It is small, when conveying said structure bottle, only need directly to realize the conveying to bottle with various Belt Drives, that is, will
Bottle is placed on the conveyer belt of motion, and bottle is conveyed by the frictional force produced between conveyor belt surface and bottom of bottle.However, this
A little bottle conveying mechanisms can not be but applied in the bottle conveying of medical biological sample, when running into the bottleneck bottle bigger than body diameter
During body, if conveyed using conventional mode of movement, it is easy for causing more serious " emitting bottle " and " bottle " phenomenon.Such as in medical treatment
It is bigger than body diameter if taking up biological sample such as blood, the test tube of urine its bottleneck in field, and by the way of manually carrying
Obviously waste time and energy.
In addition, in the biology sample detection field detected to urine etc., any one color can with RGB come
Represent, rgb color pattern is a kind of color standard of industrial quarters, be by red (R), green (G), blue (B) three Color Channels
Change and their superpositions each other obtain miscellaneous color, RGB is to represent red, green, blue three to lead to
The color in road, this standard almost includes all colours that human eyesight can perceive, and is at present with most wide cie system of color representation cie
One of system.Rgb color pattern is distributed in the range of one 0~255 with the RGB component that RGB models are each pixel in image
Intensity level.For example:Pure red R values are that 255, G values are that 0, B values are 0;Tri- values of R, G, B of grey are equal (except 0 and 255);In vain
R, G, B of color are 255;R, G, B of black are 0.RGB image is only using three kinds of colors, so that it may so that they are according to difference
Ratio mixing, on screen reappear 16777216 kinds of colors.And identification of the human eye to color and shade is very smart
Thin and complicated, it can identify thousands of kinds of colors, but can only offer an explanation the gray scale aberration (bibliography for not going out 4-6Bit:《China
Stereoscopy and graphical analysis》In September, 2002, volume 7, the 3rd phase《The Pseudo Col ored Image of medical image》).
The related detection project of many medical science can pass through the optical characteristics of measurement detection sample in itself or sample and certain
Detection reagent is planted, such as such as immunochromatographiassay assay reagent, dry chemical detection reagent, chemiluminescence detection reagent, optics after reaction
The change of characteristic and obtain, some dependence gross visualizations carry out sentence read result, and some use instrumentations carry out quantitative interpretation knot
Really, it is clear that gross visualization lacks accuracy and uniformity, and existing most of instrumentations have cost height, convenience difference etc.
Problem.Have at present and replace special instrument to read detection target with smart mobile phone and tablet personal computer with camera function, it is this
Scheme reduces cost, improves the convenience used, but the difference of individual type, the difference of photoenvironment, photo angle and
The factors such as the difference and hand tremor of distance can all influence the accuracy of testing result.
Conveyed and detecting system in the urgent need to that need to look for a kind of collection conveying, be detected on integral biological sample at present..
The content of the invention
In order to overcome the defect of prior art, an object of the present invention is to provide a kind of biological sample conveying and detection system
System, it includes test tube conveying device and portable field detection terminal.
The present invention also aims to provide it is a kind of it is simple and compact for structure, with low cost, can accept with suspended structure
Test tube, active conveying bottle be reliable and stable and the adjustable test tube conveying device of active conveying bottle speed.
The present invention also aims to provide a kind of portable field detection terminal and method, can be greatly reduced makes
With cost, property easy to use is improved, and the accuracy and uniformity of professional detection device can be kept.
In order to solve the above technical problems, the present invention uses following technical scheme:
A kind of biological sample conveying and detecting system, it includes test tube conveying device and portable field detection terminal.
A kind of test tube conveying device, including frame and the guide assembly and test tube drive component that are fixed in frame, institute
The test tube accommodating cavity and the bottleneck above test tube accommodating cavity that stating guide assembly includes arranging along the test tube direction of motion are hung
Portion, the test tube drive component is located at the lower section of test tube accommodating cavity and produces the driving force that test tube is moved by being rubbed with test tube.
As a further improvement on the present invention:
The test tube drive component includes pressing plate and the conveyer belt being set around on synchronous pulley, and the pressing plate and conveyer belt are in
Symmetry shape is arranged in the both sides of test tube.
The frame is provided with more than the one piece adjustable plate provided for pressing plate towards test tube direction thrust.
Mounting hole is offered on the adjustable plate, the adjustable plate is fixed on by the clamping screw being arranged in mounting hole
In frame.
One side on the pressing plate towards test tube is provided with elastic pressure section.In addition, the present invention also provides a kind of portable
Site Detection terminal, it include power switch, battery cavities, upper and lower cover connection strap, main control board, upper lid, camera bracket,
Supply hub, test card jack, lower cover, imaging unit.
Further, the imaging unit has shell, and inside sets camera, camera bracket, circuit board, the shooting
Head bracket is two, is correspondingly arranged at the both sides of the interior forward end, the battery compartment is arranged on the Internal back end, described to take the photograph
As head sets the top of two camera brackets, the circuit board is arranged on the upper of the battery compartment and the camera
Side.
Further, the shell of the imaging unit includes upper lid, middle cover and lower cover, and the upper lid includes top surface and four
Individual side, the lower cover includes bottom surface and four sides, and the middle cover includes a side and peripheral frame, the side of the middle cover
Covered respectively with the upper lid and lower cover, the peripheral frame of the middle cover and the side of the upper lid and the lower cover are covered, described
Reagent card plug, the reagent card spigot insertion detection target fixator are provided with the side of middle cover.
Further, the upper top surface of the upper lid be provided with instrument switch hole, the instrument switch hole be provided with it is described
The switch of instrument, the switch is connected with the circuit board.
Further, be provided with external power supply jack in the side of the lower cover, in the external power supply jack with it is described
Battery compartment is connected, and the bottom surface of the lower cover is provided with battery cabin cap.
Further, the imaging unit also includes bluetooth equipment.
It also includes manipulation unit and image analyzing unit and used cooperatively with portable field detection terminal, portable field
Detection terminal is spatially separated from manipulation unit and image analyzing unit, and imaging unit passes through Near Field Communication with manipulation unit
Method is connected, and manipulation unit is connected with image analyzing unit by network communication method, and imaging unit is used for shot detection target,
Image analyzing unit is used to analyze the digital picture clapped and draws testing result, and manipulation unit is used to manipulate whole detection instrument
Device.
Main operational principle is that it is single that imaging unit sends images to manipulation by imaging unit collection image by detection card
Member has such as mobile phone or pad to be received with transfer function instrument or equipment, is connect by the APP on mobile phone or pad
The image sended over by imaging unit, uploads to image analyzing unit such as cloud computer, is clapped according to cloud computer analysis
Digital picture and draw testing result, finally feed back on mobile phone or pad quickly to draw the result of detection.
Imaging unit herein can also be any imaging unit known in the art, such as excellent to step this tech.
Advantages of the present invention:
Compared with prior art, the advantage of the invention is that:The present invention test tube conveying device, it is simple and compact for structure, into
This is cheap, by suspension part that test tube is mounted on guide assembly, and in the case where the frictional force of test tube drive component drives i.e.
The conveying of test tube can be achieved, active conveying bottle is reliable and stable and regulation of active conveying bottle speed is very convenient.And the detection means of the present invention then has
Have:1st, remote control and regulation, is easy to management.Traditional detection instrument needs detection sample and analytical instrument near work, and the present invention is real
The separation of sample collection instrument and data analyzer is showed, the remote detection to sample is realized by network data base.In addition, net
Network data backstage can also carry out remote calibration using standard color comparison card to every sample collection instrument, prevent because ageing equipment is drawn
The error risen.2nd, it is cost-effective.Under the management on unified backstage, sample collection instrument is only needed to during detection, be can be achieved with multinomial
The detection of index, cost is greatlyd save compared with traditional a full set of tester.
Brief description of the drawings
Fig. 1 is dimensional structure diagram of the present invention in application example;
Fig. 2 is overlooking the structure diagram of the present invention in application example.
Fig. 3 is that a kind of digital imagery detection of the present invention is the structural representation of instrument;
Fig. 4 is that a kind of Fig. 3 digital imagery detection is the internal structure schematic diagram of instrument;
Fig. 5 is test card schematic diagram;
Fig. 6 be the collection obtained according to cloud database go out test chart as;
Fig. 7 is the position for searching out test card test reference line;
Fig. 8 is the reagent card before and after sample-adding;
Fig. 9 is the image collecting device for inserting reagent card;
Figure 10 is that mobile phone A PP opens interface.
Embodiment
In order that the purpose of the present invention, technical scheme and advantage are more clearly understood, it is right below in conjunction with drawings and Examples
The present invention is further elaborated;It should be appreciated that specific embodiment described herein is only to explain the present invention, not
For limiting the present invention.
Embodiment
As depicted in figs. 1 and 2, a kind of biological sample conveying and detecting system, it includes test tube conveying device and portable
Site Detection terminal, wherein the test tube conveying device of the present invention, including frame 10 and the guide assembly 1 that is fixed in frame 10
With test tube drive component, guide assembly 1 includes the test tube accommodating cavity 101 arranged along the direction of motion of test tube 5 and held positioned at test tube
The bottleneck suspension part 102 of the top of chamber 101 is put, test tube drive component is located at the lower section of test tube accommodating cavity 101 and by being rubbed with test tube 5
Wipe the driving force for producing test tube motion.The bottle-delivery end of guide assembly 1 can be connected with thumb wheel 9 or other bottle-conveying mechanisms, and test tube is housed
Chamber 101 is enclosed to set by some blocks of plates to be formed.
In the present embodiment, test tube drive component includes pressing plate 6 and the conveyer belt 7 being set around on synchronous pulley 11, pressing plate 6
The both sides of test tube 5 are arranged in symmetry shape with conveyer belt 7, synchronous pulley 11 is rotated by so as to transmission actuator
Band 7 is moved, and conveyer belt 7 contacts generation frictional force with test tube 5 and drives test tube 5 to be moved forward along test tube accommodating cavity 101.Pressing plate
One side on 6 towards test tube 5 is provided with elastic pressure section 8.
Frame 10 is provided with more than the one piece adjustable plate 12 provided for pressing plate 6 towards the direction thrust of test tube 5.Adjustable plate 12
On offer mounting hole 3, adjustable plate 12 is fixed in frame 10 by the clamping screw 2 being arranged in mounting hole 3, can pass through waist
Shape hole 3 adjusts the distance between pressing plate 6 and conveyer belt 7, and then adjustable plate 12 is transmitted to the thrust size of pressing plate 6 and regulation
Size with the frictional force produced between 7 and test tube 5.The direction that mounting hole 13 is opened up is vertical with the direction of motion of test tube 5.
As shown in Figure 3-4, it is that the biological sample conveying of the present invention and the portable field of detecting system detect terminal, including
Imaging unit, manipulation unit and image analyzing unit, the imaging unit, manipulation unit and image analyzing unit spatially divide
From imaging unit is connected with manipulation unit by Near Field Communication method, and manipulation unit is logical by network with image analyzing unit
Communication method is connected, and imaging unit is used for shot detection target, and image analyzing unit is used to analyze the digital picture clapped and drawn
Testing result, manipulation unit is used to manipulate whole detection instrument.
Further, the imaging unit has shell, and inside sets camera, camera bracket 8, circuit board 7 and battery
Storehouse 6, the camera bracket 8 is two, is correspondingly arranged at the both sides of the interior forward end, and the battery compartment 6 is arranged on described
Internal back end, the camera is arranged on the top of two camera brackets 8, and the circuit board 7 is arranged on the battery
Storehouse 6 and the top of the camera.
Further, the shell of the imaging unit includes upper lid 3, middle cover 2 and lower cover 5, and the upper lid 3 includes upper top surface
With four sides, the lower cover 5 includes bottom surface and four sides, and the middle cover 2 includes a side and peripheral frame, the middle cover
2 side is covered with the upper lid 3 and lower cover 5 respectively, the peripheral frame of the middle cover 2 and the side of the upper lid 3 and the lower cover 5
Cover, insertion detection target fixator at reagent card plug 9, the reagent card plug 9 is provided with the side of middle cover 2.
Further, the upper top surface of the upper lid 3 is provided with instrument switch hole 1, the instrument switch hole 1 and set
The switch of instrument is stated, the switch is connected with the circuit board 7.
Further, it is provided with the side of the lower cover 5 in external power supply jack 4, the external power supply jack 4 and institute
State battery compartment 6 to connect, the bottom surface of the lower cover 5 is provided with battery cabin cap.
Further, the imaging unit also includes bluetooth equipment, and the imaging unit passes through the bluetooth equipment and institute
Manipulation unit is stated to be connected.
Further, described control unit is that APP is provided with mobile phone, mobile phone, and mobile phone A PP is imaged single by Bluetooth control
Member carries out IMAQ to sample.
Further, described image analytic unit is cloud computer.
Interstitialcellstimulating hormone (ICSH) (LH) concentration in the detection women body of example one
Vertically it is added dropwise 1. urina sanguinis 3 is dripped on LH reagent cards, after candidate agent card reacts 15 minutes in insertion imaging unit.
2. mobile phone A PP is opened, and mobile phone is bound with imaging unit by bluetooth, clicked on vertical on mobile phone interface
Test, test starts, test philosophy is as follows:
Reagent card is entered in the coverage of test camera, and instrument carries out the IMAQ of reagent card.Collect
Image is sent to cloud computer by network, and the latter is handled image using face recognition technology.
The first step is automatically found the long side of test card peep hole, the interface origin position Automatic-searching shot from camera,
Using immunological test card film and the color change feature of plastic testing card shell, the position letter on the long side of peep hole of test card is determined
Breath, to determine the upper edge (Fig. 5) of test pictures collection, further according to collecting test card picture corresponding in cloud database
Width information, gathers out test chart as (Fig. 6).
Second step is finding the long side of test card peep hole and is collecting test chart as on the basis of, utilizes image recognition of face
Technology, the position of Automatic-searching test card test reference line from right to left on image, if Automatic-searching is beyond finding scope
When do not find test reference line also, being just defined as this test crash, (test definition of immune chromatograph testing card is if do not had
Test reference line occurs for this test crash);Otherwise the peak edge of response line (including reference line and p-wire) is sought
Look for (Fig. 5), image software carries out tangent line calculating in response line (including reference line and p-wire) position to peak edge, according to peak side
The rate of change of edge tangent line finds the beginning and end of response line (including reference line and p-wire).In response line (including reference line
And p-wire) beginning and end region to color carry out layered method RGB unit area average values, deduct background, calculate
Real RGB numerical value, further according to standard gray angle value equation, RGB number ranges and RGB calculation formula, is finally calculated corresponding
GOD values.
3. after test terminates, LH test results are read from mobile phone A PP.
Example two:Detect human chorionic gonadotrophin concentration
Vertically it is added dropwise 1. urina sanguinis 3 is dripped on HCG type agents cards, candidate agent card inserts excellent Mai Sike after reacting 15 minutes
In instrument (Fig. 8 is the reagent card before and after sample-adding, and Fig. 9 is image collecting device-excellent this tech advanced in years of insertion reagent card).
2. mobile phone A PP is opened into (interface such as Figure 10), and mobile phone is bound with excellent this tech that steps by bluetooth, clicked on
" testing immediately ", test starts.
3. after test terminates, HCG test results are read by mobile phone A pp.