CN108070642B - 提高dnb双末端测序质量的方法和dnb双末端测序方法和试剂盒 - Google Patents
提高dnb双末端测序质量的方法和dnb双末端测序方法和试剂盒 Download PDFInfo
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- CN108070642B CN108070642B CN201710349201.6A CN201710349201A CN108070642B CN 108070642 B CN108070642 B CN 108070642B CN 201710349201 A CN201710349201 A CN 201710349201A CN 108070642 B CN108070642 B CN 108070642B
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- NHVNXKFIZYSCEB-XLPZGREQSA-N dTTP Chemical compound O=C1NC(=O)C(C)=CN1[C@@H]1O[C@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)[C@@H](O)C1 NHVNXKFIZYSCEB-XLPZGREQSA-N 0.000 claims abstract description 38
- ISAKRJDGNUQOIC-UHFFFAOYSA-N Uracil Chemical compound O=C1C=CNC(=O)N1 ISAKRJDGNUQOIC-UHFFFAOYSA-N 0.000 claims abstract description 32
- 102000004190 Enzymes Human genes 0.000 claims abstract description 23
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- 238000003786 synthesis reaction Methods 0.000 claims abstract description 20
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- 230000015572 biosynthetic process Effects 0.000 claims abstract description 15
- 238000004873 anchoring Methods 0.000 claims abstract description 12
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- ZHNUHDYFZUAESO-UHFFFAOYSA-N Formamide Chemical group NC=O ZHNUHDYFZUAESO-UHFFFAOYSA-N 0.000 claims description 12
- 239000000203 mixture Substances 0.000 claims description 9
- 239000002773 nucleotide Substances 0.000 claims description 9
- 125000003729 nucleotide group Chemical group 0.000 claims description 9
- 239000003153 chemical reaction reagent Substances 0.000 claims description 7
- 239000003795 chemical substances by application Substances 0.000 claims 2
- 108020004414 DNA Proteins 0.000 description 26
- 230000003321 amplification Effects 0.000 description 13
- 238000005516 engineering process Methods 0.000 description 13
- 238000003199 nucleic acid amplification method Methods 0.000 description 13
- 238000006073 displacement reaction Methods 0.000 description 8
- 108020004638 Circular DNA Proteins 0.000 description 5
- 239000012634 fragment Substances 0.000 description 5
- 238000005096 rolling process Methods 0.000 description 4
- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 description 3
- 102000006943 Uracil-DNA Glycosidase Human genes 0.000 description 3
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- 238000003776 cleavage reaction Methods 0.000 description 2
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- 229910052710 silicon Inorganic materials 0.000 description 2
- 239000010703 silicon Substances 0.000 description 2
- FWMNVWWHGCHHJJ-SKKKGAJSSA-N 4-amino-1-[(2r)-6-amino-2-[[(2r)-2-[[(2r)-2-[[(2r)-2-amino-3-phenylpropanoyl]amino]-3-phenylpropanoyl]amino]-4-methylpentanoyl]amino]hexanoyl]piperidine-4-carboxylic acid Chemical compound C([C@H](C(=O)N[C@H](CC(C)C)C(=O)N[C@H](CCCCN)C(=O)N1CCC(N)(CC1)C(O)=O)NC(=O)[C@H](N)CC=1C=CC=CC=1)C1=CC=CC=C1 FWMNVWWHGCHHJJ-SKKKGAJSSA-N 0.000 description 1
- KKZFLSZAWCYPOC-VPENINKCSA-N Deoxyribose 5-phosphate Chemical compound O[C@H]1C[C@H](O)[C@@H](COP(O)(O)=O)O1 KKZFLSZAWCYPOC-VPENINKCSA-N 0.000 description 1
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- 239000005549 deoxyribonucleoside Substances 0.000 description 1
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6869—Methods for sequencing
Abstract
Description
Claims (14)
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
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CN201611012549 | 2016-11-17 | ||
CN2016110125498 | 2016-11-17 |
Publications (2)
Publication Number | Publication Date |
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CN108070642A CN108070642A (zh) | 2018-05-25 |
CN108070642B true CN108070642B (zh) | 2020-10-27 |
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CN201710349201.6A Active CN108070642B (zh) | 2016-11-17 | 2017-05-17 | 提高dnb双末端测序质量的方法和dnb双末端测序方法和试剂盒 |
Country Status (2)
Country | Link |
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CN (1) | CN108070642B (zh) |
HK (1) | HK1250756A1 (zh) |
Families Citing this family (3)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN111286527B (zh) * | 2018-12-10 | 2024-03-19 | 深圳华大智造科技股份有限公司 | 合成dna纳米球互补链的方法和测序方法 |
CN114641581A (zh) * | 2019-10-28 | 2022-06-17 | 深圳华大智造科技股份有限公司 | Dna接头寡核苷酸 |
CN113774120A (zh) * | 2020-06-09 | 2021-12-10 | 深圳华大智造科技有限公司 | Dnb双末端测序方法 |
Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2010148039A2 (en) * | 2009-06-15 | 2010-12-23 | Complete Genomics, Inc. | Methods and compositions for long fragment read sequencing |
CN104039438A (zh) * | 2011-11-02 | 2014-09-10 | 考利达基因组股份有限公司 | 用于稳定核酸阵列的处理方法 |
CN105986324A (zh) * | 2015-02-11 | 2016-10-05 | 深圳华大基因研究院 | 环状小rna文库构建方法及其应用 |
CN106148482A (zh) * | 2015-03-24 | 2016-11-23 | 深圳华大基因研究院 | 一种适用于小型测序仪的测序方法 |
-
2017
- 2017-05-17 CN CN201710349201.6A patent/CN108070642B/zh active Active
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2018
- 2018-08-09 HK HK18110241.6A patent/HK1250756A1/zh unknown
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
WO2010148039A2 (en) * | 2009-06-15 | 2010-12-23 | Complete Genomics, Inc. | Methods and compositions for long fragment read sequencing |
CN104039438A (zh) * | 2011-11-02 | 2014-09-10 | 考利达基因组股份有限公司 | 用于稳定核酸阵列的处理方法 |
CN105986324A (zh) * | 2015-02-11 | 2016-10-05 | 深圳华大基因研究院 | 环状小rna文库构建方法及其应用 |
CN106148482A (zh) * | 2015-03-24 | 2016-11-23 | 深圳华大基因研究院 | 一种适用于小型测序仪的测序方法 |
Non-Patent Citations (2)
Title |
---|
Biomimetic DNA nanoballs for oligonucleotide delivery;Mi-Gyeong Kim等;《Biomaterials》;20150605;155-163 * |
Denoising DNA deep sequencing data—high-throughput sequencing errors and their correction;David Laehnemann等;《Briefings in Bioinformatics》;20160131;154–179 * |
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HK1250756A1 (zh) | 2019-01-11 |
CN108070642A (zh) | 2018-05-25 |
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