CN108060105A - A kind of star vegetable Alteromonad with high flocculation activity - Google Patents
A kind of star vegetable Alteromonad with high flocculation activity Download PDFInfo
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- CN108060105A CN108060105A CN201810103135.9A CN201810103135A CN108060105A CN 108060105 A CN108060105 A CN 108060105A CN 201810103135 A CN201810103135 A CN 201810103135A CN 108060105 A CN108060105 A CN 108060105A
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- star
- vegetable
- flocculation activity
- alteromonad
- strain
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
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- C—CHEMISTRY; METALLURGY
- C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
- C02F3/00—Biological treatment of water, waste water, or sewage
- C02F3/34—Biological treatment of water, waste water, or sewage characterised by the microorganisms used
- C02F3/348—Biological treatment of water, waste water, or sewage characterised by the microorganisms used characterised by the way or the form in which the microorganisms are added or dosed
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Microbiology (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Biotechnology (AREA)
- Biodiversity & Conservation Biology (AREA)
- Hydrology & Water Resources (AREA)
- Environmental & Geological Engineering (AREA)
- Water Supply & Treatment (AREA)
- Medicinal Chemistry (AREA)
- Tropical Medicine & Parasitology (AREA)
- Virology (AREA)
- Biomedical Technology (AREA)
- Separation Of Suspended Particles By Flocculating Agents (AREA)
Abstract
The invention discloses a kind of star vegetable Alteromonads with high flocculation activity, the strain classification Latin is named as Alteromonas stellipolaris, strain number is HM2017 12 002, China Microbial Culture Preservation Commission's common micro-organisms collection is preserved on November 30th, 2017, deposit number is CGMCC NO：14971.Present invention firstly discovers that star vegetable Alteromonad has the function of high flocculation activity, for microbial treatment sewage a kind of new way is provided.
Description
Technical field
The present invention relates to technical field of water pollution treatment, more particularly to a kind of star vegetable Alteromonas with high flocculation activity
Bacterium.
Background technology
In numerous wastewater treatment methods, flocculating setting occupies extremely important status in water process.Flocculant is one
Class can make suspended particulate not degradable in liquid (grain size 10-7~10-3Cm the substance for) agglomerating, settling.Industry Waste both domestic and external
Water process accounts for 60%~70% using the ratio of flocculation treatment, and originally water and wastewater industry nearly all uses flocculation treatment as water purification
Means.But chemical floc easily causes environment secondary pollution, microbial flocculant is that have harmless, flocculating effect
Good, easily biological-degradable, the novel flocculant for not causing the features such as secondary pollution, are currently widely used in water harnessing.
And in research in recent years, microbial flocculant also has good prospect in terms of harmful algae is administered, wide
General concern.Therefore the active bacterial strain of source Yu Haiyang of the screening with high flocculation activity is not only to carry out sewage cleaning treatment
Important process content also provides possibility for Disaster And Prevention Measures of Red Tides processing, marine ecosystem reparation.
The content of the invention
It is an object of the invention to provide a kind of star vegetable Alteromonads with high flocculation activity, are that microbial treatment is dirty
Water provides more multipath.
The technical solution adopted by the present invention to solve the technical problems is：
A kind of star vegetable Alteromonad with high flocculation activity, the strain classification Latin are named as Alteromonas
Stellipolaris, strain number HM2017-12-002 are preserved in Chinese microorganism strain on November 30th, 2017
Preservation committee common micro-organisms collection, deposit number are CGMCC NO：14971.
Alteromonas stellipolaris also have Chinese to be translated into South Pole ocean Oligotrophic bacteria, and the present invention is through sieving
Choosing, is found that star vegetable Alteromonad (Alteromonas stellipolaris) has the function of high flocculation activity, is for the first time
Microbial treatment sewage provides a kind of new way.
A kind of purposes of star vegetable Alteromonad with high flocculation activity as the biological flocculant for administering sewage.
The beneficial effects of the invention are as follows：The present invention is found that star vegetable Alteromonad for the first time by screening
(Alteromonas stellipolaris) has the function of high flocculation activity, is provided for microbial treatment sewage a kind of new
Approach.
Description of the drawings
Fig. 1 is the micro- enlarged drawing of bacterial strain of the present invention (under oil mirror, 10 × 100 times).
Fig. 2 is the tablet separation figure (2216E tablets) of bacterial strain of the present invention.
Specific embodiment
Below by specific embodiment, technical scheme is described in further detail.
In the present invention, if not refering in particular to, used raw material and equipment etc. are commercially available or commonly used in the art.
Method in following embodiments is the conventional method of this field unless otherwise instructed.
Embodiment：
1. sample collection：
Using professional oceanographic research ship from China's Area of The East China Sea, surface seawater is gathered.Experiment on Microbiology on research vessel
Room, it is interior at the first time to carry out live Experiment on Microbiology.The seawater dilution collected carries out tablet coating by certain multiple.This reality
It tests water sample dilution spread and selects 2216E tablets, each measuring point equality applies 3 tablets.
2. single strain separating：
Culture after a certain period of time, after single bacterium colony is grown in tablet, (size, color, shape is distinguished with the morphological feature of bacterium colony
Shape, side together whether, whether protrusion, bacterium solution quality, whether have it is swim) streak inoculation separation.Continue after single bacterium colony grows up to
Streak inoculation purifying is until be kept completely separate.
A single bacterium colony separation inoculation two tablets and Liquid Culture test tube (10ml fluid nutrient mediums), for conservation and
Remaining items experiment.
Media Components：
(1) fluid nutrient medium：Glucose 20g/L, urea 0.5g/L, ammonium sulfate 0.2g/L, yeast extract 0.5g/L, phosphoric acid
Hydrogen dipotassium 1g/L, potassium dihydrogen phosphate 1g/L, agar 15g/L and surplus ageing seawater or artificial seawater；Adjusting pH is 7.2-7.4,
115 DEG C of high pressure sterilization 30min.
(2) 2216E tablets：Peptone 5g/L, yeast extract 1g/L, high ferric phosphate 0.1g/L, agar 20g/L；Surplus is aged
Seawater；Adjusting pH is 7.6,121 DEG C of high pressure sterilization 20min.
3. flocculation activity is tested：
Target bacterium solution culture：
Liquid Culture test tube is put into shaking table culture one week first, training objective bacterium solution.To improve conventional efficient, we are first
First using Tube propagation as primary dcreening operation.The opposite strain for having flocculation activity is filtered out, is further inoculated into 250ml conical flasks again
Secondary culture assay.
Flocculation activity measures：
1st, the aqueous suspension ofkaolin 93ml of 4g/L, magnetic stirring apparatus moderate-speed mixer 30 minutes or more are configured.
2nd, the CaCl of 5ml 1% (m/v) is added in2, adjust PH to 7.5.
3rd, 2ml samples to be tested are added in, be mixed (first quick stirring 1min (180r/min), then mix slowly 5 minutes
(120r/min).It avoids smashing alumen ustum and floccule, controls the identical reaction time.
4th, suspension, 722 type visible spectrophotometers measure at 550nm at 5mm below absorption liquid level after standing 10min
Absorbance A (while using 2ml pure water sample is replaced to measure absorbance as control and be denoted as B, daily experiment all needs the measure same day pure
Water absorbance).Two parallel groups are done with group experiment.
5th, calculate：Flocculation activity represents as follows with flocculating rate (%)：
Flocculating rate (%)=(B-A)/B*100%
Flocculating rate is with higher flocculation activity more than 80%.
As a result and subsequently：
After primary dcreening operation, being due to Tube propagation is insufficient, flocculating rate is generally relatively low.So subsequently it is with the addition of range estimation
The artificial screening (poor, deviation, medium, preferable, good) of its coagulation is used as screening reference simultaneously with flocculating rate screening, will wherein
The flocculating rate bacterial screening good higher than 40% flocculating effect goes out culture, other strains are abandoned.
The preferable strain strain of flocculating effect after primary dcreening operation is inoculated into the 250ml conical flasks equipped with 30ml fluid nutrient mediums and is put
Enter shaking table culture one week.Then measuring flocculation activity is carried out according to above step, carrying out three Duplicate Samples with group measures.It obtains
Obtain final accurate result.
It is that HM12-002 flocculation activities are 91% that gained, which has the bacterial strain of high flocculation activity, and strain morphology is in bacterium colony size
Deng, white, subcircular, side are uneven, central have fold protrusion, quality semisolid.The micro- enlarged drawing of bacterial strain and spread plate are shown in attached
Fig. 1,2.
4. strain idenfication
Bacterial strain HM2017-12-002 serves the auspicious sound biology Co., Ltd in sea and carries out strain idenfication, and 16s rRNA are sequenced and are tied
Fruit uses the Nucleotide BLAST in BLAST to carry out sequence alignment, and comparing result is as shown in table 1.
Table 1
Qualification result is as follows：
HM2017-12-002 bacterial strains are star vegetable Alteromonad (Alteromonas stellipolaris).
In addition, present invention discover that HM2017-12-002 bacterial strains with high flocculation activity can be as administering sewage
Biological flocculant is applied to waste water control.
Embodiment described above is a kind of preferable scheme of the present invention, and not the present invention is made in any form
Limitation also has other variants and remodeling on the premise of without departing from the technical solution recorded in claim.
Claims (2)
1. a kind of star vegetable Alteromonad with high flocculation activity, which is characterized in that the strain classification Latin is named as
Alteromonas stellipolaris, strain number HM2017-12-002, on November 30th, 2017 is preserved in
The Microbiological Culture Collection committee of state common micro-organisms collection, deposit number are CGMCC NO：14971.
2. a kind of star vegetable Alteromonad with high flocculation activity as described in claim 1 is as the biology wadding for administering sewage
The purposes of solidifying agent.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810103135.9A CN108060105B (en) | 2018-02-01 | 2018-02-01 | Alteromonas stelleri with high flocculation activity |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810103135.9A CN108060105B (en) | 2018-02-01 | 2018-02-01 | Alteromonas stelleri with high flocculation activity |
Publications (2)
| Publication Number | Publication Date |
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| CN108060105A true CN108060105A (en) | 2018-05-22 |
| CN108060105B CN108060105B (en) | 2020-12-15 |
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| CN201810103135.9A Active CN108060105B (en) | 2018-02-01 | 2018-02-01 | Alteromonas stelleri with high flocculation activity |
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Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20110129902A1 (en) * | 2009-11-27 | 2011-06-02 | Chung-Ang University Industry-Academy Cooperation Foundation | Microbial strain Alteromonas SP. SN2 for degrading polycyclic aromatic hydrocarbon |
| CN104745518A (en) * | 2015-04-16 | 2015-07-01 | 厦门大学 | Method for preparing biological flocculant by utilizing marine Alteromonas sp. H-6 |
| CN106145586A (en) * | 2016-08-02 | 2016-11-23 | 长兴瑷晟环保科技有限公司 | A kind of biological modified method of deeply dehydrating sludge |
| CN107641610A (en) * | 2017-09-18 | 2018-01-30 | 浙江海洋大学 | One plant of ocean Halomonas and prepare the method for flocculant with it |
-
2018
- 2018-02-01 CN CN201810103135.9A patent/CN108060105B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20110129902A1 (en) * | 2009-11-27 | 2011-06-02 | Chung-Ang University Industry-Academy Cooperation Foundation | Microbial strain Alteromonas SP. SN2 for degrading polycyclic aromatic hydrocarbon |
| CN104745518A (en) * | 2015-04-16 | 2015-07-01 | 厦门大学 | Method for preparing biological flocculant by utilizing marine Alteromonas sp. H-6 |
| CN106145586A (en) * | 2016-08-02 | 2016-11-23 | 长兴瑷晟环保科技有限公司 | A kind of biological modified method of deeply dehydrating sludge |
| CN107641610A (en) * | 2017-09-18 | 2018-01-30 | 浙江海洋大学 | One plant of ocean Halomonas and prepare the method for flocculant with it |
Non-Patent Citations (1)
| Title |
|---|
| 杜宁: "南极微生物絮凝剂产生菌的筛选、分子鉴定及适应性研究", 《化学与生物工程》 * |
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| CN108060105B (en) | 2020-12-15 |
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