CN108048556A - 一种用于检测骨质疏松症的试剂盒 - Google Patents
一种用于检测骨质疏松症的试剂盒 Download PDFInfo
- Publication number
- CN108048556A CN108048556A CN201810012621.XA CN201810012621A CN108048556A CN 108048556 A CN108048556 A CN 108048556A CN 201810012621 A CN201810012621 A CN 201810012621A CN 108048556 A CN108048556 A CN 108048556A
- Authority
- CN
- China
- Prior art keywords
- osteoporosis
- kit
- sample
- bone
- detection
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 208000001132 Osteoporosis Diseases 0.000 title claims abstract description 46
- 239000000523 sample Substances 0.000 claims abstract description 50
- 238000011895 specific detection Methods 0.000 claims abstract 2
- 238000001514 detection method Methods 0.000 claims description 19
- 238000000034 method Methods 0.000 claims description 12
- 238000002474 experimental method Methods 0.000 abstract description 3
- 230000002265 prevention Effects 0.000 abstract description 2
- 230000001225 therapeutic effect Effects 0.000 abstract description 2
- 210000000988 bone and bone Anatomy 0.000 description 23
- 238000011160 research Methods 0.000 description 23
- 238000004458 analytical method Methods 0.000 description 14
- 239000008280 blood Substances 0.000 description 13
- 230000037182 bone density Effects 0.000 description 13
- 210000004369 blood Anatomy 0.000 description 12
- 238000006243 chemical reaction Methods 0.000 description 12
- 108090000623 proteins and genes Proteins 0.000 description 12
- 208000002193 Pain Diseases 0.000 description 10
- 241000282414 Homo sapiens Species 0.000 description 9
- 238000010197 meta-analysis Methods 0.000 description 9
- 206010017076 Fracture Diseases 0.000 description 7
- 238000003908 quality control method Methods 0.000 description 7
- 208000010392 Bone Fractures Diseases 0.000 description 6
- 238000005516 engineering process Methods 0.000 description 6
- 208000029725 Metabolic bone disease Diseases 0.000 description 5
- 238000012408 PCR amplification Methods 0.000 description 5
- 210000002436 femur neck Anatomy 0.000 description 5
- 102000002260 Alkaline Phosphatase Human genes 0.000 description 4
- 108020004774 Alkaline Phosphatase Proteins 0.000 description 4
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 4
- 230000015572 biosynthetic process Effects 0.000 description 4
- 239000011575 calcium Substances 0.000 description 4
- 229910052791 calcium Inorganic materials 0.000 description 4
- 239000003153 chemical reaction reagent Substances 0.000 description 4
- 238000009826 distribution Methods 0.000 description 4
- 230000000694 effects Effects 0.000 description 4
- 125000006853 reporter group Chemical group 0.000 description 4
- 230000008901 benefit Effects 0.000 description 3
- 239000003795 chemical substances by application Substances 0.000 description 3
- 201000010099 disease Diseases 0.000 description 3
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 3
- 230000002068 genetic effect Effects 0.000 description 3
- 238000003205 genotyping method Methods 0.000 description 3
- 229910052500 inorganic mineral Inorganic materials 0.000 description 3
- 239000011707 mineral Substances 0.000 description 3
- 102000054765 polymorphisms of proteins Human genes 0.000 description 3
- 238000010791 quenching Methods 0.000 description 3
- 230000000171 quenching effect Effects 0.000 description 3
- 230000009467 reduction Effects 0.000 description 3
- 238000012163 sequencing technique Methods 0.000 description 3
- 230000005186 women's health Effects 0.000 description 3
- 238000001712 DNA sequencing Methods 0.000 description 2
- 101000946179 Homo sapiens Cilia- and flagella-associated protein 20 Proteins 0.000 description 2
- 102000003982 Parathyroid hormone Human genes 0.000 description 2
- 108090000445 Parathyroid hormone Proteins 0.000 description 2
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 2
- 239000007853 buffer solution Substances 0.000 description 2
- 230000008859 change Effects 0.000 description 2
- DDRJAANPRJIHGJ-UHFFFAOYSA-N creatinine Chemical compound CN1CC(=O)NC1=N DDRJAANPRJIHGJ-UHFFFAOYSA-N 0.000 description 2
- 238000003745 diagnosis Methods 0.000 description 2
- 238000013399 early diagnosis Methods 0.000 description 2
- 239000012634 fragment Substances 0.000 description 2
- 230000006870 function Effects 0.000 description 2
- 238000012268 genome sequencing Methods 0.000 description 2
- 238000011337 individualized treatment Methods 0.000 description 2
- 238000007689 inspection Methods 0.000 description 2
- 230000000366 juvenile effect Effects 0.000 description 2
- 238000012417 linear regression Methods 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 239000003147 molecular marker Substances 0.000 description 2
- 230000035772 mutation Effects 0.000 description 2
- 230000000869 mutational effect Effects 0.000 description 2
- 239000002773 nucleotide Substances 0.000 description 2
- 125000003729 nucleotide group Chemical group 0.000 description 2
- 239000000199 parathyroid hormone Substances 0.000 description 2
- 229960001319 parathyroid hormone Drugs 0.000 description 2
- 210000005259 peripheral blood Anatomy 0.000 description 2
- 239000011886 peripheral blood Substances 0.000 description 2
- 239000011574 phosphorus Substances 0.000 description 2
- 229910052698 phosphorus Inorganic materials 0.000 description 2
- 108090000765 processed proteins & peptides Proteins 0.000 description 2
- 108010048734 sclerotin Proteins 0.000 description 2
- 210000002966 serum Anatomy 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 208000024891 symptom Diseases 0.000 description 2
- 238000012360 testing method Methods 0.000 description 2
- 238000012795 verification Methods 0.000 description 2
- UDGUGZTYGWUUSG-UHFFFAOYSA-N 4-[4-[[2,5-dimethoxy-4-[(4-nitrophenyl)diazenyl]phenyl]diazenyl]-n-methylanilino]butanoic acid Chemical compound COC=1C=C(N=NC=2C=CC(=CC=2)N(C)CCCC(O)=O)C(OC)=CC=1N=NC1=CC=C([N+]([O-])=O)C=C1 UDGUGZTYGWUUSG-UHFFFAOYSA-N 0.000 description 1
- WCKQPPQRFNHPRJ-UHFFFAOYSA-N 4-[[4-(dimethylamino)phenyl]diazenyl]benzoic acid Chemical compound C1=CC(N(C)C)=CC=C1N=NC1=CC=C(C(O)=O)C=C1 WCKQPPQRFNHPRJ-UHFFFAOYSA-N 0.000 description 1
- 108700028369 Alleles Proteins 0.000 description 1
- 206010002383 Angina Pectoris Diseases 0.000 description 1
- -1 BHQ3 Chemical compound 0.000 description 1
- 208000008035 Back Pain Diseases 0.000 description 1
- 208000020084 Bone disease Diseases 0.000 description 1
- 206010065687 Bone loss Diseases 0.000 description 1
- 206010008479 Chest Pain Diseases 0.000 description 1
- 102100034701 Cilia- and flagella-associated protein 20 Human genes 0.000 description 1
- 102000012422 Collagen Type I Human genes 0.000 description 1
- 108010022452 Collagen Type I Proteins 0.000 description 1
- 206010010214 Compression fracture Diseases 0.000 description 1
- 206010011224 Cough Diseases 0.000 description 1
- 241001269238 Data Species 0.000 description 1
- ZAHDXEIQWWLQQL-IHRRRGAJSA-N Deoxypyridinoline Chemical compound OC(=O)[C@@H](N)CCCC[N+]1=CC(O)=C(C[C@H](N)C([O-])=O)C(CC[C@H](N)C(O)=O)=C1 ZAHDXEIQWWLQQL-IHRRRGAJSA-N 0.000 description 1
- KCXVZYZYPLLWCC-UHFFFAOYSA-N EDTA Chemical compound OC(=O)CN(CC(O)=O)CCN(CC(O)=O)CC(O)=O KCXVZYZYPLLWCC-UHFFFAOYSA-N 0.000 description 1
- HTTJABKRGRZYRN-UHFFFAOYSA-N Heparin Chemical compound OC1C(NC(=O)C)C(O)OC(COS(O)(=O)=O)C1OC1C(OS(O)(=O)=O)C(O)C(OC2C(C(OS(O)(=O)=O)C(OC3C(C(O)C(O)C(O3)C(O)=O)OS(O)(=O)=O)C(CO)O2)NS(O)(=O)=O)C(C(O)=O)O1 HTTJABKRGRZYRN-UHFFFAOYSA-N 0.000 description 1
- 101000864646 Homo sapiens Dickkopf-related protein 1 Proteins 0.000 description 1
- 206010049949 Intercostal neuralgia Diseases 0.000 description 1
- 208000008930 Low Back Pain Diseases 0.000 description 1
- 208000030136 Marchiafava-Bignami Disease Diseases 0.000 description 1
- 102000004067 Osteocalcin Human genes 0.000 description 1
- 108090000573 Osteocalcin Proteins 0.000 description 1
- 238000009004 PCR Kit Methods 0.000 description 1
- LCYXYLLJXMAEMT-SAXRGWBVSA-N Pyridinoline Chemical compound OC(=O)[C@@H](N)CCC1=C[N+](C[C@H](O)CC[C@H](N)C([O-])=O)=CC(O)=C1C[C@H](N)C(O)=O LCYXYLLJXMAEMT-SAXRGWBVSA-N 0.000 description 1
- 208000005392 Spasm Diseases 0.000 description 1
- 208000007103 Spondylolisthesis Diseases 0.000 description 1
- 102000007591 Tartrate-Resistant Acid Phosphatase Human genes 0.000 description 1
- 108010032050 Tartrate-Resistant Acid Phosphatase Proteins 0.000 description 1
- 210000001766 X chromosome Anatomy 0.000 description 1
- 208000005298 acute pain Diseases 0.000 description 1
- 230000003321 amplification Effects 0.000 description 1
- 230000003262 anti-osteoporosis Effects 0.000 description 1
- 238000012098 association analyses Methods 0.000 description 1
- 230000002308 calcification Effects 0.000 description 1
- 159000000007 calcium salts Chemical class 0.000 description 1
- 210000003164 cauda equina Anatomy 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 230000006835 compression Effects 0.000 description 1
- 238000007906 compression Methods 0.000 description 1
- 238000007796 conventional method Methods 0.000 description 1
- 238000012937 correction Methods 0.000 description 1
- 238000010219 correlation analysis Methods 0.000 description 1
- 229940109239 creatinine Drugs 0.000 description 1
- 230000001186 cumulative effect Effects 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 230000007850 degeneration Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- 230000004064 dysfunction Effects 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 210000003414 extremity Anatomy 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
- 102000054766 genetic haplotypes Human genes 0.000 description 1
- 230000003694 hair properties Effects 0.000 description 1
- 208000019622 heart disease Diseases 0.000 description 1
- 230000004217 heart function Effects 0.000 description 1
- 229960002897 heparin Drugs 0.000 description 1
- 229920000669 heparin Polymers 0.000 description 1
- 238000004128 high performance liquid chromatography Methods 0.000 description 1
- 230000003907 kidney function Effects 0.000 description 1
- 238000002372 labelling Methods 0.000 description 1
- 230000003902 lesion Effects 0.000 description 1
- 230000003908 liver function Effects 0.000 description 1
- 210000003141 lower extremity Anatomy 0.000 description 1
- 210000004705 lumbosacral region Anatomy 0.000 description 1
- 239000003550 marker Substances 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000003387 muscular Effects 0.000 description 1
- 230000000926 neurological effect Effects 0.000 description 1
- 238000003199 nucleic acid amplification method Methods 0.000 description 1
- 210000000056 organ Anatomy 0.000 description 1
- 201000008482 osteoarthritis Diseases 0.000 description 1
- 230000000849 parathyroid Effects 0.000 description 1
- 238000009527 percussion Methods 0.000 description 1
- 238000000513 principal component analysis Methods 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 238000004393 prognosis Methods 0.000 description 1
- 238000003753 real-time PCR Methods 0.000 description 1
- 210000000664 rectum Anatomy 0.000 description 1
- 230000008439 repair process Effects 0.000 description 1
- 230000002441 reversible effect Effects 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 210000001032 spinal nerve Anatomy 0.000 description 1
- 238000007619 statistical method Methods 0.000 description 1
- ABZLKHKQJHEPAX-UHFFFAOYSA-N tetramethylrhodamine Chemical compound C=12C=CC(N(C)C)=CC2=[O+]C2=CC(N(C)C)=CC=C2C=1C1=CC=CC=C1C([O-])=O ABZLKHKQJHEPAX-UHFFFAOYSA-N 0.000 description 1
- 210000000115 thoracic cavity Anatomy 0.000 description 1
- 210000001519 tissue Anatomy 0.000 description 1
- 238000011282 treatment Methods 0.000 description 1
- 230000002485 urinary effect Effects 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
- C12Q1/6883—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for diseases caused by alterations of genetic material
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q2600/00—Oligonucleotides characterized by their use
- C12Q2600/156—Polymorphic or mutational markers
Landscapes
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- Analytical Chemistry (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Pathology (AREA)
- Immunology (AREA)
- Microbiology (AREA)
- Molecular Biology (AREA)
- Biotechnology (AREA)
- Biophysics (AREA)
- Physics & Mathematics (AREA)
- Biochemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
Description
Claims (6)
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201810012621.XA CN108048556B (zh) | 2018-01-05 | 2018-01-05 | 一种用于检测骨质疏松症的试剂盒 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201810012621.XA CN108048556B (zh) | 2018-01-05 | 2018-01-05 | 一种用于检测骨质疏松症的试剂盒 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN108048556A true CN108048556A (zh) | 2018-05-18 |
CN108048556B CN108048556B (zh) | 2019-10-11 |
Family
ID=62126586
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201810012621.XA Active CN108048556B (zh) | 2018-01-05 | 2018-01-05 | 一种用于检测骨质疏松症的试剂盒 |
Country Status (1)
Country | Link |
---|---|
CN (1) | CN108048556B (zh) |
Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN105200151A (zh) * | 2015-10-29 | 2015-12-30 | 北京泱深生物信息技术有限公司 | Tcf21基因作为疾病诊断标志物的用途 |
CN106868125A (zh) * | 2017-02-20 | 2017-06-20 | 苏州大学 | 用于幼年型骨质疏松症检测的试剂盒 |
-
2018
- 2018-01-05 CN CN201810012621.XA patent/CN108048556B/zh active Active
Patent Citations (2)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN105200151A (zh) * | 2015-10-29 | 2015-12-30 | 北京泱深生物信息技术有限公司 | Tcf21基因作为疾病诊断标志物的用途 |
CN106868125A (zh) * | 2017-02-20 | 2017-06-20 | 苏州大学 | 用于幼年型骨质疏松症检测的试剂盒 |
Non-Patent Citations (1)
Title |
---|
HGSV: "Cor18555_SNV_20070510.chr4_175621212", 《DBSNP》 * |
Also Published As
Publication number | Publication date |
---|---|
CN108048556B (zh) | 2019-10-11 |
Similar Documents
Publication | Publication Date | Title |
---|---|---|
CN105256039A (zh) | 骨质疏松症诊断标志物 | |
CN103205488B (zh) | 一种预测强直性脊柱炎易感性的方法和试剂 | |
CN110277138B (zh) | 一种激素性股骨头坏死易感基因检测方法 | |
CN108220426B (zh) | 一种用于检测骨质疏松症的试剂盒 | |
CN108048556B (zh) | 一种用于检测骨质疏松症的试剂盒 | |
CN107988359B (zh) | 一种用于检测骨质疏松症的试剂盒 | |
CN108220425B (zh) | 一种用于检测骨质疏松症的试剂盒 | |
CN107937518B (zh) | 一种用于检测骨质疏松症的试剂盒 | |
CN107988360B (zh) | 一种用于检测骨质疏松症的试剂盒 | |
CN108048558B (zh) | 一种用于检测骨质疏松症的试剂盒 | |
CN108048555A (zh) | 一种用于检测骨质疏松症的试剂盒 | |
CN108034715A (zh) | 一种用于检测骨质疏松症的试剂盒 | |
CN107937517A (zh) | 一种用于检测骨质疏松症的试剂盒 | |
CN103882112B (zh) | 一种预测强直性脊柱炎易感性的试剂及方法 | |
CN103695549B (zh) | 预测强直性脊柱炎易感性的试剂 | |
CN107058497A (zh) | 一种通过检测rs16981092来特异性检测肥胖症的试剂盒 | |
CN103725781B (zh) | 一种预测强直性脊柱炎易感性的试剂盒及方法 | |
CN109055542A (zh) | 预测失重骨丢失腰椎骨密度下降风险的方法与dna甲基化标志物 | |
CN106591476A (zh) | 一种通过检测rs2069126来特异性检测肥胖症的试剂盒 | |
CN109762889A (zh) | 一种基于rs6470763检测幼年型骨质疏松症的试剂盒 | |
CN112824539A (zh) | 一种通过rs571770来特异性检测少肌症的试剂盒 | |
CN109762888A (zh) | 一种基于rs6866306检测幼年型骨质疏松症的试剂盒 | |
CN109762891A (zh) | 一种基于rs7532173检测幼年型骨质疏松症的试剂盒 | |
CN109762892A (zh) | 一种基于rs72906356检测幼年型骨质疏松症的试剂盒 | |
CN109762887A (zh) | 一种基于rs73403830检测幼年型骨质疏松症的试剂盒 |
Legal Events
Date | Code | Title | Description |
---|---|---|---|
PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
TA01 | Transfer of patent application right | ||
TA01 | Transfer of patent application right |
Effective date of registration: 20190828 Address after: 201616 7th Floor, Lane 11, 4855 Guangfulin Road, Songjiang District, Shanghai Applicant after: Shanghai Fucoo Biomedical Technology Co.,Ltd. Address before: No. 4315, 4th Floor, Building 7, Fengxian Middle Road, Haidian District, Beijing, 100000 Applicant before: BEIJING YUEHAO TECHNOLOGY DEVELOPMENT CO.,LTD. |
|
GR01 | Patent grant | ||
GR01 | Patent grant | ||
PE01 | Entry into force of the registration of the contract for pledge of patent right | ||
PE01 | Entry into force of the registration of the contract for pledge of patent right |
Denomination of invention: A reagent kit for detecting osteoporosis Effective date of registration: 20231205 Granted publication date: 20191011 Pledgee: Industrial Bank Co.,Ltd. Shanghai Minhang sub branch Pledgor: Shanghai Fucoo Biomedical Technology Co.,Ltd. Registration number: Y2023310000803 |