CN108048436A - 一种皂素清洁化生产黄姜工业用复合酶制剂 - Google Patents

一种皂素清洁化生产黄姜工业用复合酶制剂 Download PDF

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CN108048436A
CN108048436A CN201711441297.5A CN201711441297A CN108048436A CN 108048436 A CN108048436 A CN 108048436A CN 201711441297 A CN201711441297 A CN 201711441297A CN 108048436 A CN108048436 A CN 108048436A
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Abstract

本发明涉及一种皂素清洁化生产黄姜工业用复合酶制剂，其特征在于是由复合纤维素酶、果胶酶、葡聚糖酶、淀粉酶、糖苷酶和植酸酶复配而成。本发明依据黄姜的组成成分，针对性的选择了上述酶复配。采用本发明的复合酶制剂生产薯蓣皂素，可促进黄姜有效成分的释放，提高收率，从本质上解决水解薯蓣皂苷所用的酸，从而降低生产成本，同时有效解决了皂素生产中的水污染问题，可减少黄姜生产企业40%的废水排放量。

Description

一种皂素清洁化生产黄姜工业用复合酶制剂
技术领域
本发明涉及生物酶制剂技术领域，特别是涉及一种皂素清洁化生产黄姜工业用复合酶制剂。
背景技术
黄姜又名穿地龙，学名盾叶薯蓣，为多年生草质藤本植物，其块茎有较高的药用和化工价值，经过加工可提炼出皂素、双稀等医药、化工用品，尤其是从黄姜中提炼出的薯蓣皂素为合成甾体类药物的基础原料药，利用它可以合成氢化可的松、强的松、地塞米松等上百种甾体激素类药物。因此黄姜被喻为“激素之母”，是激素类药物必不可少的成份，并广泛应用于化妆、保健、避孕镇痛、麻醉等类药物，是一种市场需求较为稳定的药材。传统的从黄姜中提取薯蓣皂素的工艺是将薯蓣粉碎后全料投入酸解，但是该方法存在着薯蓣中的有效成分利用率低，成本高，对环境污染大等缺陷，通常50多吨薯蓣干药材仅能生产1吨皂素，其耗盐酸达19吨左右，用水洗酸解物至中性，产生(酸0.13当量以上，含有机物2.8％)高浓度有机废水高达1500吨左右。
发明内容
本发明的目的就在于提供克服上述现有技术的缺陷，提供一种促使黄姜有效成分的释放，提高收率，降低皂素生产成本，实现皂素清洁化生产的一种皂素清洁化生产黄姜工业用复合酶制剂。
本发明的另一目的是上述一种皂素清洁化生产黄姜工业用复合酶制剂的具体应用方法。
本发明是通过下述技术方案实现的：
一种一种皂素清洁化生产黄姜工业用复合酶制剂，其特征在于是由下述成分按重量百分比组成：
复合纤维素酶20％—30％，
果胶酶12％—25％，
葡聚糖酶10％—15％，
淀粉酶25％—35％，
糖苷酶5％—10％，
植酸酶3％—5％；
所述的复合纤维素酶是由50％的纤维素酶和50％的木聚糖酶组成，以重量百分比计；
所述的果胶酶是由60％的果胶酯酶和40％的果胶裂解酶组成，以重量百分比计；
所述的淀粉酶是由70％的中温α-淀粉酶、20％的真菌α-淀粉酶和10％的普鲁兰酶组成，以重量百分比计；
所述的糖苷酶是60％由β-葡萄糖苷酶和40％鼠李糖苷酶组成，以重量百分比计；
所述的葡聚糖酶为β—葡聚糖酶。
上述一种皂素清洁化生产黄姜工业用复合酶制剂的应用方法，其工艺步骤为：
a.原料经复合酶法预处理：鲜黄姜去杂，用锤片式粉碎机加三倍量水破碎成浆，加入万分之五至千分之五的一种皂素清洁化生产黄姜工业用复合酶制剂，在室温、自然PH值条件下酶解1—6小时，
b.有机溶剂提取：在酶解后的原料中加入30-80％的乙醇，以体积百分比计，提取1—6小时，固液分离，料渣重复提取一次，
c.回收溶剂，提取物干燥：回收乙醇，在30-80℃将提取物烘干，
d.复合酶解皂苷为苷元：提取物用3倍量水稀释，加入万分之五到千分之五的一种皂素清洁化生产黄姜工业用复合酶制剂，在42℃-60℃，pH值4.5-6.5酶解2-40小时，
e.烘干酶解物：酶解物烘干后，按皂素提取常规工艺提取、浓缩、结晶得到皂素成品。
检测表明：黄姜(盾叶薯蓣)中含植物纤维33％—40％，淀粉45％—55％，水溶物质9％—13％，皂素含量2％—3％，因此，本发明针对性的选择了纤维素酶、果胶酶、葡聚糖酶、淀粉酶、植酸酶和糖苷酶，它们的作用原理如下：
1.足够的复合纤维素酶：能够水解原料中的纤维素，破坏其链状结构及其半纤维素构成的网状细胞壁结构，释放出其中被包裹的有效成分皂苷。
2.足够的果胶酶及β-葡聚糖酶：通过果胶酶及β-葡聚糖酶的协同作用，降解因植物体内果胶及葡聚糖的存在而导致物理通道堵塞造成水分无法从分子通道中渗出，导致提取物粘度大，通过黄姜工业用复合酶打开分子间通道，降解粘性杂质，降低过滤粘度。
3.足够的淀粉酶：黄姜中淀粉含量为45％—55％，黄姜粉碎后薯蓣皂苷被大量的淀粉包裹，淀粉酶可将淀粉液化，有利于有机溶剂对皂苷的提取。
4.适量的植酸酶：协同复合纤维素酶及果胶酶共同作用于植物细胞壁，能够改变果胶质等大多数胶体物质，具有亲水性质的纤维及半纤维表面电荷与水中正电荷的相互吸引力。
5.适量的糖苷酶：能够水解薯蓣皂苷中的糖苷键，用于皂苷的生物转化。
通过上述组合所获得的一种皂素清洁化生产黄姜工业用复合酶制剂的工艺参数为：
作用温度：15-60℃(或室温)pH值：4.0-7.0(或自然pH)
作用时间：2-48h添加量：500g-5000g/吨物料
搅拌：40％回流漕液搅拌作用结束pH值：3.0-3.5
本发明将上述复合酶制剂用于薯蓣皂素的生产，其不同于传统工艺将薯蓣全量投入酸解，造成收率低，生产成本高，大量污水带来的环境污染等弊端，也不同于几种新工艺用机械破碎薯蓣细胞壁，破碎率低，耗能高等缺陷，本工艺方法采用连续复合酶法，可促进黄姜有效成分的释放，提高收率，从本质上解决水解薯蓣皂苷所用的酸，从而降低生产成本，同时有效解决了皂素生产中的水污染问题，减少黄姜生产企业40％的废水排放量。
应用本发明可大大降低企业生产周期，黄姜水解后的废水可用于发酵酒精，提高综合利用率。
应用本发明可将黄姜提取皂素后的废渣综合利用，用于全价配合饲料，降低企业生产成本，增加收率。
具体实施方式
实施例1
本发明一种皂素清洁化生产黄姜工业用复合酶制剂的具体配方如下，以重量百分比计：复合纤维素酶30％(其中纤维素酶50％，木聚糖酶50％)，果胶酶15％(其中果胶酯酶60％，果胶裂解酶40％)，淀粉酶25％(其中中温α-淀粉酶70％，真菌α-淀粉酶20％，普鲁兰酶10％)，β—葡聚糖酶15％，糖苷酶10％(其中β-葡萄糖苷酶60％，鼠李糖苷酶40％)，植酸酶5％。
制备方法为：将原料酶分别经微滤膜过滤，再按比例进入自动混合机复配，然后通过精滤膜过滤、定量灌装，成为产品。
实施例2
本发明一种皂素清洁化生产黄姜工业用复合酶制剂的具体配方如下，以重量百分比计：复合纤维素酶25％(其中纤维素酶50％，木聚糖酶50％)，果胶酶25％(其中果胶酯酶60％，果胶裂解酶40％)，淀粉酶35％(其中中温α-淀粉酶70％，真菌α-淀粉酶20％，普鲁兰酶10％)，β—葡聚糖酶10％，糖苷酶5％(其中β-葡萄糖苷酶60％，鼠李糖苷酶40％)，植酸酶5％。
制备方法与例1相同。
实施例3
本发明一种皂素清洁化生产黄姜工业用复合酶制剂的具体配方如下，以重量百分比计：复合纤维素酶20％(其中纤维素酶50％，木聚糖酶50％)，果胶酶20％(其中果胶酯酶60％，果胶裂解酶40％)，淀粉酶35％(其中中温α-淀粉酶70％，真菌α-淀粉酶20％，普鲁兰酶10％)，β—葡聚糖酶14％，糖苷酶8％(其中β-葡萄糖苷酶60％，鼠李糖苷酶40％)，植酸酶3％。
制备方法与例1相同。
实施例4
本发明一种皂素清洁化生产黄姜工业用复合酶制剂的具体配方如下，以重量百分比计：复合纤维素酶28％(其中纤维素酶50％，木聚糖酶50％)，果胶酶12％(其中果胶酯酶60％，果胶裂解酶40％)，淀粉酶30％(其中中温α-淀粉酶70％，真菌α-淀粉酶20％，普鲁兰酶10％)，β—葡聚糖酶15％，糖苷酶10％(其中β-葡萄糖苷酶60％，鼠李糖苷酶40％)，植酸酶5％。
制备方法与例1相同。
实施方式1
上述一种皂素清洁化生产黄姜工业用复合酶制剂的应用方法，其工艺步骤为：
a.原料复合酶法预处理：鲜薯蓣去杂，用锤片式粉碎机加三倍量水破碎成浆，加入万分之五至千分之五的一种皂素清洁化生产黄姜工业用复合酶制剂，在室温、自然PH值条件下酶解1—6小时，此过程的目的是通过生物手段破坏植物细胞壁，释放薯蓣皂苷，降解大分子物质，
b.有机溶剂提取：在酶解后的原料中加入30-80％的乙醇，以体积百分比计，提取1—6小时，固液分离，料渣重复提取一次。
c.回收溶剂，提取物干燥：回收乙醇，在30-80℃将提取物烘干，
d.复合酶解皂苷为苷元：提取物用3倍量水稀释，加入万分之五到千分之五的复合酶制剂，在42℃-60℃，pH值4.5-6.5酶解2-40小时，此过程可使皂苷转化为皂苷元，苷与苷元的转化率达90％以上，
e.烘干酶解物：酶解物烘干后，按皂素提取常规工艺提取、浓缩、结晶得到皂素成品。

Claims (7)

1.一种皂素清洁化生产黄姜工业用复合酶制剂，其特征在于是由下述成分按重量百分比组成：
复合纤维素酶20％—30％，
果胶酶12％—25％，
葡聚糖酶10％—15％，
淀粉酶25％—35％，
糖苷酶5％—10％，
植酸酶3％—5％。
2.按照权利要求1所述的皂素清洁化生产黄姜工业用复合酶制剂，其特征在于所述的复合纤维素酶是由50％的纤维素酶和50％的木聚糖酶组成，以重量百分比计。
3.按照权利要求1所述的皂素清洁化生产黄姜工业用复合酶制剂，其特征在于所述的果胶酶是由60％的果胶酯酶和40％的果胶裂解酶组成，以重量百分比计。
4.按照权利要求1所述的皂素清洁化生产黄姜工业用复合酶制剂，其特征在于所述的淀粉酶是由70％的中温α-淀粉酶、20％的真菌α-淀粉酶和10％的普鲁兰酶组成，以重量百分比计。
5.按照权利要求1所述的皂素清洁化生产黄姜工业用复合酶制剂，其特征在于所述的糖苷酶是60％由β-葡萄糖苷酶和40％鼠李糖苷酶组成，以重量百分比计。
6.按照权利要求1所述的皂素清洁化生产黄姜工业用复合酶制剂，其特征在于所述的葡聚糖酶为β—葡聚糖酶。
7.一种如权利要求1所述的皂素清洁化生产黄姜工业用复合酶制剂的应用方法，其工艺步骤为：
a.原料经复合酶法预处理：鲜黄姜去杂，用锤片式粉碎机加三倍量水破碎成浆，加入万分之五至千分之五的皂素清洁化生产黄姜工业用复合酶制剂，在室温、自然PH值条件下酶解1—6小时，
b.有机溶剂提取：在酶解后的原料中加入30-80％的乙醇，以体积百分比计，提取1—6小时，固液分离，料渣重复提取一次，
c.回收溶剂，提取物干燥：回收乙醇，在30-80℃将提取物烘干，
d.复合酶解皂苷为苷元：提取物用3倍量水稀释，加入万分之五到千分之五的皂素清洁化生产黄姜工业用复合酶制剂，在42℃-60℃，pH值4.5-6.5酶解2-40小时，
e.烘干酶解物：酶解物烘干后，按皂素提取常规工艺提取、浓缩、结晶得到皂素成品。
CN201711441297.5A 2017-12-27 2017-12-27 一种皂素清洁化生产黄姜工业用复合酶制剂 Withdrawn CN108048436A (zh)

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* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN109457009A (zh) * 2018-12-28 2019-03-12 河北肽都生物科技有限公司 一种小分子黄芪螯合肽的制备方法
CN109457009B (zh) * 2018-12-28 2021-08-03 河北肽都生物科技集团有限公司 一种小分子黄芪螯合肽的制备方法

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