CN108041594A - The preparation method of Patinopecten yessoensis Male reproduction zymolyte-beta carotene lotion - Google Patents
The preparation method of Patinopecten yessoensis Male reproduction zymolyte-beta carotene lotion Download PDFInfo
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- CN108041594A CN108041594A CN201711211983.3A CN201711211983A CN108041594A CN 108041594 A CN108041594 A CN 108041594A CN 201711211983 A CN201711211983 A CN 201711211983A CN 108041594 A CN108041594 A CN 108041594A
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- zymolyte
- patinopecten yessoensis
- male reproduction
- beta carotene
- peptide solution
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- 241000237516 Mizuhopecten yessoensis Species 0.000 title claims abstract description 35
- 239000006210 lotion Substances 0.000 title claims abstract description 33
- 238000002360 preparation method Methods 0.000 title claims abstract description 14
- 239000011648 beta-carotene Substances 0.000 title claims description 31
- 229960002747 betacarotene Drugs 0.000 title claims description 31
- 108090000765 processed proteins & peptides Proteins 0.000 claims abstract description 40
- 230000007062 hydrolysis Effects 0.000 claims abstract description 24
- 238000006460 hydrolysis reaction Methods 0.000 claims abstract description 24
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 22
- 239000003921 oil Substances 0.000 claims abstract description 21
- 235000019198 oils Nutrition 0.000 claims abstract description 21
- PXIPVTKHYLBLMZ-UHFFFAOYSA-N Sodium azide Chemical compound [Na+].[N-]=[N+]=[N-] PXIPVTKHYLBLMZ-UHFFFAOYSA-N 0.000 claims abstract description 20
- 229920001213 Polysorbate 20 Polymers 0.000 claims abstract description 10
- 239000002285 corn oil Substances 0.000 claims abstract description 10
- 235000005687 corn oil Nutrition 0.000 claims abstract description 10
- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 claims abstract description 10
- 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 claims abstract description 10
- 239000006185 dispersion Substances 0.000 claims abstract description 8
- 238000010438 heat treatment Methods 0.000 claims abstract description 8
- OENHQHLEOONYIE-UKMVMLAPSA-N all-trans beta-carotene Natural products CC=1CCCC(C)(C)C=1/C=C/C(/C)=C/C=C/C(/C)=C/C=C/C=C(C)C=CC=C(C)C=CC1=C(C)CCCC1(C)C OENHQHLEOONYIE-UKMVMLAPSA-N 0.000 claims description 20
- 235000013734 beta-carotene Nutrition 0.000 claims description 20
- TUPZEYHYWIEDIH-WAIFQNFQSA-N beta-carotene Natural products CC(=C/C=C/C=C(C)/C=C/C=C(C)/C=C/C1=C(C)CCCC1(C)C)C=CC=C(/C)C=CC2=CCCCC2(C)C TUPZEYHYWIEDIH-WAIFQNFQSA-N 0.000 claims description 20
- OENHQHLEOONYIE-JLTXGRSLSA-N β-Carotene Chemical compound CC=1CCCC(C)(C)C=1\C=C\C(\C)=C\C=C\C(\C)=C\C=C\C=C(/C)\C=C\C=C(/C)\C=C\C1=C(C)CCCC1(C)C OENHQHLEOONYIE-JLTXGRSLSA-N 0.000 claims description 19
- 239000003995 emulsifying agent Substances 0.000 abstract description 9
- 230000006641 stabilisation Effects 0.000 abstract description 6
- 238000011105 stabilization Methods 0.000 abstract description 6
- 230000000050 nutritive effect Effects 0.000 abstract description 4
- 239000000243 solution Substances 0.000 description 39
- 239000000839 emulsion Substances 0.000 description 15
- 239000000047 product Substances 0.000 description 14
- 238000003860 storage Methods 0.000 description 9
- 238000009826 distribution Methods 0.000 description 7
- 108090000623 proteins and genes Proteins 0.000 description 5
- 102000004169 proteins and genes Human genes 0.000 description 5
- 230000000694 effects Effects 0.000 description 4
- 235000015097 nutrients Nutrition 0.000 description 4
- 239000000126 substance Substances 0.000 description 4
- 241000237509 Patinopecten sp. Species 0.000 description 3
- 235000013305 food Nutrition 0.000 description 3
- 230000014759 maintenance of location Effects 0.000 description 3
- 238000000034 method Methods 0.000 description 3
- 238000007254 oxidation reaction Methods 0.000 description 3
- 235000020637 scallop Nutrition 0.000 description 3
- 235000015170 shellfish Nutrition 0.000 description 3
- 241000251468 Actinopterygii Species 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- 230000000975 bioactive effect Effects 0.000 description 2
- 210000000481 breast Anatomy 0.000 description 2
- 230000015556 catabolic process Effects 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 238000006731 degradation reaction Methods 0.000 description 2
- 238000001514 detection method Methods 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 235000019688 fish Nutrition 0.000 description 2
- 210000002149 gonad Anatomy 0.000 description 2
- 238000001727 in vivo Methods 0.000 description 2
- 239000007788 liquid Substances 0.000 description 2
- 239000000463 material Substances 0.000 description 2
- 239000007764 o/w emulsion Substances 0.000 description 2
- 230000003647 oxidation Effects 0.000 description 2
- 239000002245 particle Substances 0.000 description 2
- FPIPGXGPPPQFEQ-UHFFFAOYSA-N 13-cis retinol Natural products OCC=C(C)C=CC=C(C)C=CC1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-UHFFFAOYSA-N 0.000 description 1
- 208000002177 Cataract Diseases 0.000 description 1
- 241000195493 Cryptophyta Species 0.000 description 1
- 241000238557 Decapoda Species 0.000 description 1
- 108010082495 Dietary Plant Proteins Proteins 0.000 description 1
- MYMOFIZGZYHOMD-UHFFFAOYSA-N Dioxygen Chemical compound O=O MYMOFIZGZYHOMD-UHFFFAOYSA-N 0.000 description 1
- 241000282326 Felis catus Species 0.000 description 1
- 229920002683 Glycosaminoglycan Polymers 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 241001125046 Sardina pilchardus Species 0.000 description 1
- 108010054866 Shellfish Proteins Proteins 0.000 description 1
- FPIPGXGPPPQFEQ-BOOMUCAASA-N Vitamin A Natural products OC/C=C(/C)\C=C\C=C(\C)/C=C/C1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-BOOMUCAASA-N 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 150000007513 acids Chemical class 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- FPIPGXGPPPQFEQ-OVSJKPMPSA-N all-trans-retinol Chemical compound OC\C=C(/C)\C=C\C=C(/C)\C=C\C1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-OVSJKPMPSA-N 0.000 description 1
- 230000003064 anti-oxidating effect Effects 0.000 description 1
- 150000001588 beta-ionone derivatives Chemical class 0.000 description 1
- 239000006227 byproduct Substances 0.000 description 1
- 201000011510 cancer Diseases 0.000 description 1
- 235000021466 carotenoid Nutrition 0.000 description 1
- 150000001747 carotenoids Chemical class 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000004945 emulsification Methods 0.000 description 1
- 238000009313 farming Methods 0.000 description 1
- 235000021323 fish oil Nutrition 0.000 description 1
- 239000000796 flavoring agent Substances 0.000 description 1
- 235000019634 flavors Nutrition 0.000 description 1
- 210000004907 gland Anatomy 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 230000028993 immune response Effects 0.000 description 1
- 230000000415 inactivating effect Effects 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 150000002632 lipids Chemical class 0.000 description 1
- 208000002780 macular degeneration Diseases 0.000 description 1
- 229910052751 metal Inorganic materials 0.000 description 1
- 239000002184 metal Substances 0.000 description 1
- 229910021645 metal ion Inorganic materials 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 230000001473 noxious effect Effects 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 239000002243 precursor Substances 0.000 description 1
- 239000003531 protein hydrolysate Substances 0.000 description 1
- 238000010791 quenching Methods 0.000 description 1
- 230000000171 quenching effect Effects 0.000 description 1
- 150000003254 radicals Chemical class 0.000 description 1
- 235000019512 sardine Nutrition 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 230000009278 visceral effect Effects 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 235000019155 vitamin A Nutrition 0.000 description 1
- 239000011719 vitamin A Substances 0.000 description 1
- 229940045997 vitamin a Drugs 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
- 238000000733 zeta-potential measurement Methods 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
- A23L29/10—Foods or foodstuffs containing additives; Preparation or treatment thereof containing emulsifiers
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/17—Amino acids, peptides or proteins
- A23L33/18—Peptides; Protein hydrolysates
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Polymers & Plastics (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Nutrition Science (AREA)
- Mycology (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Botany (AREA)
- Cosmetics (AREA)
- Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
Abstract
The invention discloses a kind of preparation methods of the Patinopecten yessoensis Male reproduction zymolyte bata-carotene lotion of stabilization, include the following steps that (1) digests Patinopecten yessoensis Male reproduction with trypsase, the zymolyte peptide solution that degree of hydrolysis is 1% is obtained, is freezed spare.(2) it is for use to be configured to oil phase by dissolving by heating in corn oil for bata-carotene；(3) certain density peptide solution with sodium azide storing solution and Tween 20 is mixed, it is mutually for use is configured to water；(4) oil phase is mutually mixed in a certain ratio with water, high speed dispersion；(5) stable Patinopecten yessoensis Male reproduction zymolyte bata-carotene lotion is obtained after high-pressure homogeneous.For the present invention by the use of Patinopecten yessoensis Male reproduction zymolyte as emulsifier, embedding bata-carotene obtains a kind of stabilization and the Patinopecten yessoensis Male reproduction zymolyte bata-carotene lotion with certain nutritive value.
Description
Technical field
The present invention relates to a kind of preparation method of lotion, more specifically to Patinopecten yessoensis Male reproduction zymolyte-
The preparation method of beta carotene lotion.
Background technology
Fat-soluble nutrients substance refers to that one kind can be dissolved in fat, and then the object being taken in, digest by human body, absorbing
Matter.Common fat-soluble nutrients substance includes fish oil, algae oil, phosphatide, liposoluble vitamin etc..In order to improve fat-soluble nutrients
For matter in the in vivo bioavailability of people, common method is that it is embedded using oil-in-water emulsion systems.
It when fat-soluble nutrients substance is transferred as core, can protect it from being degraded, commonly use albumen as breast
Agent plays stabilization on oil-water interfaces by reducing interfacial tension and increase steric hindrance；And protein zymolyte has
Some oxidation resistances can inhibit lipid oxidation, can equally play the role of stable emulsion.It is common with vegetable protein at present
And its zymolyte studies the stability of the emulsion system of its structure, the lotion system constructed for aquatic products zymolyte as emulsifier
System, such as common fish origin, sardine, stigma fish for cats etc..Emulsion system is constructed as emulsifier using shellfish protein enzymolysis object
Research is also rarely reported.
Patinopecten yessoensis (Patinopecten yessoensis) belongs to filter-feeding cold water bivalve, and China mainly cultivates area
For Liaoning Province and the part sea area of Shandong Province.Patinopecten yessoensis has the characteristics that individual big, yield height, Bei Zhu are fertile.Patinopecten yessoensis
Gonad is up to more than 60% as edible position, protein content, is the good source for developing active peptide.Scallop is processed
Containing bioactive ingredients such as abundant protein, polysaccharide, glycosaminoglycan and unrighted acids in by-product, in this seminar
In early-stage study, the external source enzyme resolvings objects such as scallop gonad, shirt rim, visceral mass with antioxidation activity have successively been obtained.
And sea-farming kind of the shellfish as China's maximum, it is very necessary that system research is carried out to its abundant protein resource.
Beta carotene be distributed in nature most extensively be also current scholar's most study carotenoid, in food industry
With it is extremely important in food scientific research.Beta carotene has the function of various biological, and there are two β-ionones in molecule
Ring, with 100% vitamin A precursor activity.In addition, its potential quenching singlet oxygen and the activity for inactivating free radical exist
Prevent also to have played larger effect in specific cancer, angiocardiopathy, macular degeneration disease, cataract and raising immune response.
Therefore, beta carotene is a kind of to the very important bioactive substance of health.
Beta carotene would generally be under the action of light, heat, enzyme, metal ion, the protein combined with metal and microorganism
Accelerated degradation causes a series of oxidation reaction, bioactivity is caused to reduce, color degradation, generates bad flavor and potential
Noxious material, bioavailability decline etc..Therefore, it is that a kind of cost is relatively low to transfer beta carotene using oil-in-water emulsion, and can
To improve the method for its water-soluble, stability and bioavailability, there is preferable application prospect in food industry.
The content of the invention
For shellfish derived Protein emulsion system, the Patinopecten yessoensis Male reproduction for providing a kind of stabilization digests the present invention
Object-beta carotene lotion.
In order to achieve the above object, the present invention provides a kind of Patinopecten yessoensis Male reproduction zymolyte-beta carotene breasts
The preparation method of liquid, comprises the following steps：
S1) Patinopecten yessoensis Male reproduction is digested with trypsase, obtains peptide solution, is freezed spare；
S2) by 50~55 DEG C of 10~20min of heating of beta carotene, it is made to be dissolved in corn oil, oil phase is configured to and treats
With；
S3) peptide solution obtained by step S1 with sodium azide storing solution and Tween 20 is mixed, it is mutually for use to be configured to water；
S4) oil phase and the water are mixed, disperseed；
S5 final lotion) is obtained after homogeneous.
Under preferred embodiment, concretely comprise the following steps：
S1) Patinopecten yessoensis Male reproduction is digested with trypsase, the peptide solution that degree of hydrolysis is 1% is obtained, freezes
It is dry spare；
S2) by 50~55 DEG C of 10~20min of heating of beta carotene, it is made to be dissolved in corn oil, oil phase is configured to and treats
With；
S3) peptide solution and the sodium azide of 0.01~0.02wt% that concentration obtained by step S1 is 10~30mg/mL are stored up
The Tween 20 of standby liquid and 0.5~1wt% are mixed, and it is mutually for use to be configured to water；
S4 oil phase and water mutually) are pressed 1:9 ratio mixing, 10000-15000r/min high speed dispersions 2-3min；
S5) 10000-12000psi is 5-7 times high-pressure homogeneous.
The present invention relates to a kind of preparation method of Patinopecten yessoensis Male reproduction zymolyte-beta carotene lotion of stabilization,
Patinopecten yessoensis Male reproduction is digested with trypsase including step (1), the zymolyte that degree of hydrolysis is 1% is obtained, freezes
It is dry spare.(2) it is for use to be configured to oil phase by dissolving by heating in corn oil for beta carotene；(3) by certain density peptide
Solution is mixed with sodium azide storing solution and Tween 20, and it is mutually for use to be configured to water；(4) by oil phase and water mutually by a certain percentage
Mixing, high speed dispersion；(5) stable Patinopecten yessoensis Male reproduction zymolyte-beta carotene lotion is obtained after high-pressure homogeneous.
By the use of Patinopecten yessoensis Male reproduction zymolyte as emulsifier, embedding beta carotene obtains a kind of stabilization and has the present invention
Patinopecten yessoensis Male reproduction zymolyte-beta carotene lotion of certain nutritive value.
The present invention uses the protolysate in shellfish source as emulsifier, while emulsion system stability is promoted
Improve the nutritive value of product；Using beta carotene as embedded material, it can be improved and digested and absorbed people is in vivo
Rate makes product have higher nutritive value.Preparation method is simple and practicable, and the lotion produced has preferable stability.
Description of the drawings
It is respectively 1% using degree of hydrolysis that Fig. 1, which is, concentration as the peptide solution and degree of hydrolysis of 10mg/mL is 1%, concentration 30mg/
The peptide solution of mL prepares the grain size distribution of product emulsion of the present invention storage 1 day at 4 DEG C.
It is respectively 1% using degree of hydrolysis that Fig. 2, which is, concentration as the peptide solution and degree of hydrolysis of 10mg/mL is 1%, concentration 30mg/
The peptide solution of mL prepares the grain size distribution of product emulsion of the present invention storage 4 days at 4 DEG C.
It is respectively 1% using degree of hydrolysis that Fig. 3, which is, concentration as the peptide solution and degree of hydrolysis of 10mg/mL is 1%, concentration 30mg/
The peptide solution of mL prepares the grain size distribution of product emulsion of the present invention storage 7 days at 4 DEG C.
It is respectively 1% using degree of hydrolysis that Fig. 4, which is, concentration as the peptide solution and degree of hydrolysis of 10mg/mL is 1%, concentration 30mg/
The peptide solution of mL prepares the grain size distribution that product emulsion of the present invention is stored 1 day in room temperature.
It is respectively 1% using degree of hydrolysis that Fig. 5, which is, concentration as the peptide solution and degree of hydrolysis of 10mg/mL is 1%, concentration 30mg/
The peptide solution of mL prepares the grain size distribution that product emulsion of the present invention is stored 4 days in room temperature.
It is respectively 1% using degree of hydrolysis that Fig. 6, which is, concentration as the peptide solution and degree of hydrolysis of 10mg/mL is 1%, concentration 30mg/
The peptide solution of mL prepares the grain size distribution that product emulsion of the present invention is stored 7 days in room temperature.
Fig. 7 be respectively with the peptide solution of concentration 10mg/mL, 30mg/mL prepare product emulsion of the present invention 4 DEG C of storages 1 day,
The average grain diameter of 4 days and 7 days.
Fig. 8 is to prepare product emulsion of the present invention respectively with the peptide solution of concentration 10mg/mL, 30mg/mL to store 1 in room temperature
My god, the average grain diameter of 4 days and 7 days.
Fig. 9 be respectively with the peptide solution of concentration 10mg/mL, 30mg/mL prepare product emulsion of the present invention 4 DEG C of storages 1 day,
The zeta potential diagrams of 4 days and 7 days.
Figure 10 is to prepare product emulsion of the present invention respectively with the peptide solution of concentration 10mg/mL, 30mg/mL to store 1 in room temperature
My god, the zeta potential diagrams of 4 days and 7 days.
Figure 11 is the photograph for the lotion storage 1 day at 4 DEG C for preparing gained with the peptide solution of concentration 10mg/mL, 30mg/mL respectively
Piece figure.
Figure 12 is the photograph for the lotion storage 4 days at 4 DEG C for preparing gained with the peptide solution of concentration 10mg/mL, 30mg/mL respectively
Piece figure.
Figure 13 is the photograph for the lotion storage 7 days at 4 DEG C for preparing gained with the peptide solution of concentration 10mg/mL, 30mg/mL respectively
Piece figure.
Specific embodiment
The present invention provides a kind of preparation method of Patinopecten yessoensis Male reproduction zymolyte-beta carotene lotion, and shrimp is taken to raze
Scallop Male reproduction and beta carotene, preparation process are as follows：
S1:Patinopecten yessoensis Male reproduction is digested with trypsase, the zymolyte that degree of hydrolysis is 1% is obtained, freezes
It is dry spare；
S2:In 50-55 DEG C, beta carotene is heated into 10-20min, it is made to be dissolved in corn oil, be configured to oil phase and treat
With；
S3:By zymolyte peptide solution and sodium azide storing solution (0.01-0.02wt%) that concentration is 10~30mg/mL with
And Tween 20 (0.5-1wt%) mixing, it is mutually for use to be configured to water；
S4:Oil phase and water are mutually pressed 1:9 ratio mixing, after mixing under 10000-15000r/min high speed dispersion 2-
3min；
S5:Homogeneous 5-7 times under 10000-12000psi high pressures.
Embodiment 1
A kind of preparation method of Patinopecten yessoensis Male reproduction zymolyte-beta carotene lotion has Patinopecten yessoensis male life
Gland and beta carotene are grown, preparation process is as follows：
S1:Patinopecten yessoensis Male reproduction is digested with trypsase, the zymolyte that degree of hydrolysis is 1% is obtained, freezes
It is dry spare.
S2:Beta carotene (0.1wt%) is heated into 20min under 50 DEG C of environment, it is made to be dissolved in corn oil, is prepared
It is for use into oil phase；
S3:By the peptide solution and sodium azide storing solution (0.01wt%) and Tween20 that concentration is 10mg/mL
(0.5wt%) is mixed, and it is mutually for use to be configured to water；
S4:Oil phase and water are mutually pressed 1:9 ratio mixing, 10000r/min high speed dispersions 3min；
S5:Homogeneous 7 times under 10000psi high pressures.Obtain stable Patinopecten yessoensis Male reproduction zymolyte-β-carrot
Plain lotion.
Embodiment 2
S1:Patinopecten yessoensis Male reproduction is digested with trypsase, the zymolyte that degree of hydrolysis is 1% is obtained, freezes
It is dry spare.
S2:By 55 DEG C of heating 10min of beta carotene (0.1wt%), it is made to be dissolved in corn oil, oil phase is configured to and treats
With；
S3:By the peptide solution and sodium azide storing solution (0.01wt%) and Tween20 that concentration is 20mg/mL
(1wt%) is mixed, and it is mutually for use to be configured to water；
S4:Oil phase and water are mutually pressed 1:9 ratio mixing, 15000r/min high speed dispersions 2min；
S5:Homogeneous 6 times under 11000psi high pressures.Obtain stable Patinopecten yessoensis Male reproduction zymolyte-β-carrot
Plain lotion.
Embodiment 3
S1:Patinopecten yessoensis Male reproduction is digested with trypsase, the zymolyte that degree of hydrolysis is 1% is obtained, freezes
It is dry spare.
S2:By 52 DEG C of heating 15min of beta carotene, it is made to be dissolved in corn oil, it is for use to be configured to oil phase；
S3:By the peptide solution and sodium azide storing solution (0.02wt%) and Tween20 that concentration is 30mg/mL
(0.5wt%) is mixed, and it is mutually for use to be configured to water；
S4:Oil phase and water are mutually pressed 1:9 ratio mixing, 13000r/min high speed dispersions 2min；
S5:Homogeneous 5 times under 12000psi high pressures.Obtain stable Patinopecten yessoensis Male reproduction zymolyte-β-carrot
Plain lotion.
Grain size and zeta potential measurement situations to embodiment product：Taking appropriate lotion, (4 DEG C are stored 1 day, 4 days with room temperature
And 7 days) dilution 100 times after, using laser particle analyzer carry out average grain diameter, the detection of particle diameter distribution and zeta current potentials.Specifically
Analysis and theoretical foundation include：
(1) using the peptide solution that concentration is 10mg/mL and 30mg/mL as emulsifier, prepare lotion and be compared point
Analysis.
(2) variation that lotion stores the grain size and zeta current potentials of 1 day, 4 days and 7 days in 4 DEG C and room temperature respectively is inquired into.
(3) lotion average grain diameter is smaller, and system is more stable.
(4) zeta current potentials absolute value is higher, and system is more stable.
In attached drawing 1~10 of the present invention, 1-1：Using degree of hydrolysis as 1%, concentration is that the peptide solution of 1% (10mg/mL) is emulsification
Agent prepares the lotion of gained；1-3：Using degree of hydrolysis as 1%, the peptide solution that concentration is 3% (30mg/mL) is that emulsifier prepares gained
Lotion；RT stores for room temperature；4 DEG C are 4 DEG C of storages；Day1, Day4, Day7 are respectively the detection after storing 1 day, 4 days and 7 days
As a result.
Figure 11~13 are degree of hydrolysis respectively for 1%, the peptide solution that concentration is 1% be emulsifier prepare the zymolyte of gained-
Beta carotene lotion and degree of hydrolysis are 1%, and the peptide solution that concentration is 3% is zymolyte-β-carrot that emulsifier prepares gained
Plain lotion stores 1 day, 4 days, the state of 7 days in 4 DEG C of environment.Left side corresponds to the 1-1 in Fig. 1~10 in figure, and right side corresponds to
1-3 in Fig. 1~10.
By detecting data comparison, lotion average grain diameter of the present invention is small, and zeta current potentials absolute value is high, i.e. present invention tool
There is higher stability.
The foregoing is only a preferred embodiment of the present invention, but protection scope of the present invention be not limited thereto,
Any one skilled in the art in the technical scope of present disclosure, technique according to the invention scheme and its
Inventive concept is subject to equivalent substitution or change, should be covered by the protection scope of the present invention.
Claims (2)
1. a kind of preparation method of Patinopecten yessoensis Male reproduction zymolyte-beta carotene lotion, which is characterized in that including with
Lower step：
S1) Patinopecten yessoensis Male reproduction is digested with trypsase, obtains peptide solution, is freezed spare；
S2) by 50~55 DEG C of 10~20min of heating of beta carotene, it is made to be dissolved in corn oil, it is for use to be configured to oil phase；
S3) peptide solution obtained by step S1 with sodium azide storing solution and Tween 20 is mixed, it is mutually for use to be configured to water；
S4) oil phase and the water are mixed, disperseed；
S5) obtained after homogeneous final.
2. the preparation method of Patinopecten yessoensis Male reproduction zymolyte-beta carotene lotion according to claim 1, special
Sign is, comprises the following steps：
S1) Patinopecten yessoensis Male reproduction is digested with trypsase, the peptide solution that degree of hydrolysis is 1% is obtained, freezes standby
With；
S2) by 50~55 DEG C of 10~20min of heating of beta carotene, it is made to be dissolved in corn oil, it is for use to be configured to oil phase；
S3 it is) peptide solution of 10~30mg/mL and the sodium azide storing solution of 0.01~0.02wt% by concentration obtained by step S1
And the Tween 20 of 0.5~1wt% is mixed, and it is mutually for use to be configured to water；
S4 oil phase and water mutually) are pressed 1:9 ratio mixing, 10000-15000r/min high speed dispersions 2-3min；
S5) 10000-12000psi is 5-7 times high-pressure homogeneous.
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| CN108835490A (en) * | 2018-07-09 | 2018-11-20 | 大连工业大学 | A kind of preparation method of rheum officinale fish-egg protein isolate-beta carotene lotion |
| CN109077316A (en) * | 2018-07-09 | 2018-12-25 | 大连工业大学 | A kind of preparation method of fishskin gelatin-benzyl isothiocyanate lotion |
| CN109601994A (en) * | 2018-09-06 | 2019-04-12 | 大连工业大学 | A kind of preparation method of scallop protein isolate-beta carotene lotion |
| CN110214933A (en) * | 2019-05-13 | 2019-09-10 | 大连工业大学 | A kind of preparation method of Patinopecten yessoensis Male reproduction zymolyte/polysaccharide mixed gel |
| CN112772923A (en) * | 2021-02-02 | 2021-05-11 | 山东农业大学 | Soybean peptide aggregate-based beta-carotene embedding emulsion and preparation method thereof |
| CN116076738A (en) * | 2022-11-30 | 2023-05-09 | 大连工业大学 | Water-in-oil-in-water double-layer emulsion embedded with peptide zinc chelate and preparation method thereof |
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| CN110214933A (en) * | 2019-05-13 | 2019-09-10 | 大连工业大学 | A kind of preparation method of Patinopecten yessoensis Male reproduction zymolyte/polysaccharide mixed gel |
| CN112772923A (en) * | 2021-02-02 | 2021-05-11 | 山东农业大学 | Soybean peptide aggregate-based beta-carotene embedding emulsion and preparation method thereof |
| CN116076738A (en) * | 2022-11-30 | 2023-05-09 | 大连工业大学 | Water-in-oil-in-water double-layer emulsion embedded with peptide zinc chelate and preparation method thereof |
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Application publication date: 20180518 |