CN106617085A - Preparation method of ulva fasciata polysaccharide-tween 20 compounded beta-carotene emulsion - Google Patents
Preparation method of ulva fasciata polysaccharide-tween 20 compounded beta-carotene emulsion Download PDFInfo
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- CN106617085A CN106617085A CN201611086778.4A CN201611086778A CN106617085A CN 106617085 A CN106617085 A CN 106617085A CN 201611086778 A CN201611086778 A CN 201611086778A CN 106617085 A CN106617085 A CN 106617085A
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- beta carotene
- sea lettuce
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- polysaccharide
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- OENHQHLEOONYIE-UKMVMLAPSA-N all-trans beta-carotene Natural products CC=1CCCC(C)(C)C=1/C=C/C(/C)=C/C=C/C(/C)=C/C=C/C=C(C)C=CC=C(C)C=CC1=C(C)CCCC1(C)C OENHQHLEOONYIE-UKMVMLAPSA-N 0.000 title claims abstract description 72
- 235000013734 beta-carotene Nutrition 0.000 title claims abstract description 72
- TUPZEYHYWIEDIH-WAIFQNFQSA-N beta-carotene Natural products CC(=C/C=C/C=C(C)/C=C/C=C(C)/C=C/C1=C(C)CCCC1(C)C)C=CC=C(/C)C=CC2=CCCCC2(C)C TUPZEYHYWIEDIH-WAIFQNFQSA-N 0.000 title claims abstract description 72
- 239000011648 beta-carotene Substances 0.000 title claims abstract description 72
- 229960002747 betacarotene Drugs 0.000 title claims abstract description 72
- OENHQHLEOONYIE-JLTXGRSLSA-N β-Carotene Chemical compound CC=1CCCC(C)(C)C=1\C=C\C(\C)=C\C=C\C(\C)=C\C=C\C=C(/C)\C=C\C=C(/C)\C=C\C1=C(C)CCCC1(C)C OENHQHLEOONYIE-JLTXGRSLSA-N 0.000 title claims abstract description 68
- 239000000839 emulsion Substances 0.000 title claims abstract description 62
- 238000002360 preparation method Methods 0.000 title claims abstract description 21
- 229920001213 Polysorbate 20 Polymers 0.000 title abstract 4
- 241000158200 Ulva fasciata Species 0.000 title abstract 4
- 239000000256 polyoxyethylene sorbitan monolaurate Substances 0.000 title abstract 4
- 229920001282 polysaccharide Polymers 0.000 claims abstract description 33
- 239000005017 polysaccharide Substances 0.000 claims abstract description 33
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 33
- 239000003921 oil Substances 0.000 claims abstract description 27
- 235000019198 oils Nutrition 0.000 claims abstract description 27
- 238000000034 method Methods 0.000 claims abstract description 16
- 238000004945 emulsification Methods 0.000 claims abstract description 10
- 239000008157 edible vegetable oil Substances 0.000 claims abstract description 7
- 238000004090 dissolution Methods 0.000 claims abstract description 5
- 239000005457 ice water Substances 0.000 claims abstract description 5
- 150000004676 glycans Chemical class 0.000 claims abstract 10
- 241000196251 Ulva arasakii Species 0.000 claims description 55
- 150000001875 compounds Chemical class 0.000 claims description 17
- 210000000481 breast Anatomy 0.000 claims description 8
- 239000000203 mixture Substances 0.000 claims description 7
- 238000010008 shearing Methods 0.000 claims description 6
- 235000012424 soybean oil Nutrition 0.000 claims description 5
- 239000003549 soybean oil Substances 0.000 claims description 5
- 125000001409 beta-carotene group Chemical group 0.000 claims description 4
- 238000002156 mixing Methods 0.000 claims description 4
- 235000019486 Sunflower oil Nutrition 0.000 claims description 2
- 240000008042 Zea mays Species 0.000 claims description 2
- 235000016383 Zea mays subsp huehuetenangensis Nutrition 0.000 claims description 2
- 235000002017 Zea mays subsp mays Nutrition 0.000 claims description 2
- 235000009973 maize Nutrition 0.000 claims description 2
- 239000002600 sunflower oil Substances 0.000 claims description 2
- 239000008346 aqueous phase Substances 0.000 abstract 2
- 239000012071 phase Substances 0.000 abstract 2
- 238000000265 homogenisation Methods 0.000 abstract 1
- 235000010486 polyoxyethylene sorbitan monolaurate Nutrition 0.000 abstract 1
- 150000004804 polysaccharides Chemical class 0.000 description 23
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 description 14
- 239000003995 emulsifying agent Substances 0.000 description 14
- 235000013305 food Nutrition 0.000 description 9
- 238000013329 compounding Methods 0.000 description 7
- 238000001704 evaporation Methods 0.000 description 6
- 239000007788 liquid Substances 0.000 description 6
- 230000000694 effects Effects 0.000 description 5
- 238000005119 centrifugation Methods 0.000 description 4
- 238000005238 degreasing Methods 0.000 description 4
- 230000001804 emulsifying effect Effects 0.000 description 4
- 238000002390 rotary evaporation Methods 0.000 description 4
- 238000003756 stirring Methods 0.000 description 4
- 244000267222 Brasenia schreberi Species 0.000 description 3
- 235000006506 Brasenia schreberi Nutrition 0.000 description 3
- 238000004458 analytical method Methods 0.000 description 3
- 239000003153 chemical reaction reagent Substances 0.000 description 3
- 230000014759 maintenance of location Effects 0.000 description 3
- 239000002245 particle Substances 0.000 description 3
- 239000004575 stone Substances 0.000 description 3
- 238000010521 absorption reaction Methods 0.000 description 2
- 102000011759 adducin Human genes 0.000 description 2
- 108010076723 adducin Proteins 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 230000015572 biosynthetic process Effects 0.000 description 2
- 235000021466 carotenoid Nutrition 0.000 description 2
- 150000001747 carotenoids Chemical class 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 239000000084 colloidal system Substances 0.000 description 2
- 230000000052 comparative effect Effects 0.000 description 2
- 239000002131 composite material Substances 0.000 description 2
- 238000001816 cooling Methods 0.000 description 2
- 230000029087 digestion Effects 0.000 description 2
- 238000004821 distillation Methods 0.000 description 2
- 239000012153 distilled water Substances 0.000 description 2
- 238000001035 drying Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 230000008020 evaporation Effects 0.000 description 2
- 238000000605 extraction Methods 0.000 description 2
- 238000004108 freeze drying Methods 0.000 description 2
- VLKZOEOYAKHREP-UHFFFAOYSA-N n-Hexane Chemical compound CCCCCC VLKZOEOYAKHREP-UHFFFAOYSA-N 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 238000006116 polymerization reaction Methods 0.000 description 2
- 238000001556 precipitation Methods 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 239000006228 supernatant Substances 0.000 description 2
- FPIPGXGPPPQFEQ-UHFFFAOYSA-N 13-cis retinol Natural products OCC=C(C)C=CC=C(C)C=CC1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-UHFFFAOYSA-N 0.000 description 1
- 241001474374 Blennius Species 0.000 description 1
- 230000005653 Brownian motion process Effects 0.000 description 1
- 229920002472 Starch Polymers 0.000 description 1
- 238000005411 Van der Waals force Methods 0.000 description 1
- FPIPGXGPPPQFEQ-BOOMUCAASA-N Vitamin A Natural products OC/C=C(/C)\C=C\C=C(\C)/C=C/C1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-BOOMUCAASA-N 0.000 description 1
- 235000010489 acacia gum Nutrition 0.000 description 1
- 239000001785 acacia senegal l. willd gum Substances 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 229920001284 acidic polysaccharide Polymers 0.000 description 1
- 150000004805 acidic polysaccharides Chemical class 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- FPIPGXGPPPQFEQ-OVSJKPMPSA-N all-trans-retinol Chemical compound OC\C=C(/C)\C=C\C=C(/C)\C=C\C1=C(C)CCCC1(C)C FPIPGXGPPPQFEQ-OVSJKPMPSA-N 0.000 description 1
- 239000003963 antioxidant agent Substances 0.000 description 1
- 230000003078 antioxidant effect Effects 0.000 description 1
- 235000006708 antioxidants Nutrition 0.000 description 1
- 239000007900 aqueous suspension Substances 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 238000005537 brownian motion Methods 0.000 description 1
- 235000013339 cereals Nutrition 0.000 description 1
- 229940099352 cholate Drugs 0.000 description 1
- BHQCQFFYRZLCQQ-OELDTZBJSA-N cholic acid Chemical compound C([C@H]1C[C@H]2O)[C@H](O)CC[C@]1(C)[C@@H]1[C@@H]2[C@@H]2CC[C@H]([C@@H](CCC(O)=O)C)[C@@]2(C)[C@@H](O)C1 BHQCQFFYRZLCQQ-OELDTZBJSA-N 0.000 description 1
- 238000004581 coalescence Methods 0.000 description 1
- 239000006071 cream Substances 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 238000000280 densification Methods 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 230000006806 disease prevention Effects 0.000 description 1
- POULHZVOKOAJMA-UHFFFAOYSA-M dodecanoate Chemical compound CCCCCCCCCCCC([O-])=O POULHZVOKOAJMA-UHFFFAOYSA-M 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 239000003792 electrolyte Substances 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000005189 flocculation Methods 0.000 description 1
- 230000016615 flocculation Effects 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 235000013376 functional food Nutrition 0.000 description 1
- 230000002496 gastric effect Effects 0.000 description 1
- 230000005484 gravity Effects 0.000 description 1
- 235000013402 health food Nutrition 0.000 description 1
- 210000000936 intestine Anatomy 0.000 description 1
- 229940070765 laurate Drugs 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 239000002736 nonionic surfactant Substances 0.000 description 1
- 239000007764 o/w emulsion Substances 0.000 description 1
- 239000007800 oxidant agent Substances 0.000 description 1
- 230000003647 oxidation Effects 0.000 description 1
- 238000007254 oxidation reaction Methods 0.000 description 1
- 230000001590 oxidative effect Effects 0.000 description 1
- 238000005191 phase separation Methods 0.000 description 1
- 239000000049 pigment Substances 0.000 description 1
- 239000002243 precursor Substances 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 230000001681 protective effect Effects 0.000 description 1
- 235000019698 starch Nutrition 0.000 description 1
- 239000008107 starch Substances 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 239000004094 surface-active agent Substances 0.000 description 1
- 238000005199 ultracentrifugation Methods 0.000 description 1
- 235000019155 vitamin A Nutrition 0.000 description 1
- 239000011719 vitamin A Substances 0.000 description 1
- 229940045997 vitamin a Drugs 0.000 description 1
- 238000003809 water extraction Methods 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K9/00—Medicinal preparations characterised by special physical form
- A61K9/10—Dispersions; Emulsions
- A61K9/107—Emulsions ; Emulsion preconcentrates; Micelles
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/01—Hydrocarbons
- A61K31/015—Hydrocarbons carbocyclic
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/06—Organic compounds, e.g. natural or synthetic hydrocarbons, polyolefins, mineral oil, petrolatum or ozokerite
- A61K47/26—Carbohydrates, e.g. sugar alcohols, amino sugars, nucleic acids, mono-, di- or oligo-saccharides; Derivatives thereof, e.g. polysorbates, sorbitan fatty acid esters or glycyrrhizin
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
- A61K47/30—Macromolecular organic or inorganic compounds, e.g. inorganic polyphosphates
- A61K47/36—Polysaccharides; Derivatives thereof, e.g. gums, starch, alginate, dextrin, hyaluronic acid, chitosan, inulin, agar or pectin
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Veterinary Medicine (AREA)
- Pharmacology & Pharmacy (AREA)
- Public Health (AREA)
- General Health & Medical Sciences (AREA)
- Animal Behavior & Ethology (AREA)
- Epidemiology (AREA)
- Medicinal Chemistry (AREA)
- Oil, Petroleum & Natural Gas (AREA)
- Molecular Biology (AREA)
- General Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Engineering & Computer Science (AREA)
- Biochemistry (AREA)
- Dispersion Chemistry (AREA)
- Inorganic Chemistry (AREA)
- Acyclic And Carbocyclic Compounds In Medicinal Compositions (AREA)
- Cosmetics (AREA)
- Fodder In General (AREA)
Abstract
The invention discloses a preparation method of an ulva fasciata polysaccharide-tween 20 compounded beta-carotene emulsion. The method comprises steps as follows: (1) ulva fasciata polysaccharide and tween 20 are dispersed in water in the mass ratio being 3: 2, and an ulva fasciata polysaccharide-tween 20 compounded solution with the mass concentration of 3%-4%, namely, an aqueous phase, is obtained; (2) an oil phase is obtained by dissolving beta-carotene in edible vegetable oil, the mass percentage of beta-carotene is 0.3%-0.5%, and the temperature is kept at 60 DEG C or below in the dissolution process; (3) the oil phase obtained in step (2) and the aqueous phase obtained in step (1) are mixed in the mass ratio being 1: (10-20) for shear emulsification, high-pressure homogenization is performed in an ice-water bath, and the beta-carotene emulsion is obtained. The method is convenient to operate, the cost is low, and the beta-carotene emulsion prepared with the method has high bioavailability, good stability and high biosafety.
Description
(1) technical field
The present invention relates to medicine, food, health food processed and applied technical field, it is related to a kind of sliver sea lettuce polysaccharide-tell
Temperature 20 compounds the preparation method of beta carotene emulsion.
(2) background technology
With economic continuous development and the raising of human living standard, the mankind are to healthy concern also more and more higher, phase
Hope by simpler mode get it is more it is daily needed for various nutriments, meet the daily demand of human body and right
The prevention of disease, functional food arises at the historic moment and obtains huge concern.Beta carotene is used as a kind of natural pigment, anti-
Oxidant and efficient vitamin A precursor, can be as a kind of safe additive application in field of food, but β-Hu Luo
The dissolubility of Bu Su it is poor, unstable and it is oxidizable the characteristic such as decompose, limit its application in food.To overcome this problem, often
Beta carotene is designed in the food transport system such as stable oil-in-water emulsion, and then is dissolved into the food of complexity and produced
In product, this is beneficial to the physiologically active for protecting carrotene and improves its absorption rate.
In recent years, food-grade emulsion is widely used in the embedding and transmission of food function composition and nutrient, is a kind of letter
Single effectively embedding transportation system emulsion.The method for now food function factor being made into emulsion is a lot, but these methods have
Some defects.β-carrot emulsion particle diameter as prepared by patent CN100571684C is little, but it is organic molten to be the use of n-hexane etc.
Agent, limits its application in food system；Patent CN1079212C, by carotenoid and the aqueous suspension of protective colloid
Liquid is heated to 180 DEG C -250 DEG C and makes carotenoid fusing, then obtains emulsion by high-pressure homogeneous, and beta carotene high temperature
Easily decompose extremely unstable, the loss of beta carotene can be caused.
Emulsifying agent is that emulsion is rely stable key, and conventional emulsifying agent has protein, polysaccharide, surfactant etc..Tell
20 (TW20) of temperature are nonionic surfactants, as emulsifying agent high concentration electrolyte and pH value change to its emulsifying capacity shadow
Very little is rung, is the excellent oil/water type emulsifying agent of a big class.Contribute to the formation of emulsion, emulsion as emulsifying agent by the use of polysorbas20
Particle diameter is little, but the interfacial film mechanical strength that dispersed phase drop surface is formed is low, and anti-droplet coalescence ability stores cycle is short.And
Polysaccharide can improve the rheological behavior of emulsion continuous phase, or form spacial framework, prevent what phase separation and gravity caused
Creaming.Sliver sea lettuce is extremely rich in natural resources in China's seaweeds, and sliver sea lettuce polysaccharide is polymeric acidic polysaccharide,
Solubility is high, with good heat endurance and absolute acid stability, possesses preferable emulsifying property.And it is low with Arabic gum high concentration
Unlike viscometric properties, sliver sea lettuce polysaccharide solution just has very high viscosity in low concentration, can weaken between oil droplet molecule
The mechanical performance of emulsion is improve after the auto-polymerization caused due to molecular brownian motion, with polysorbas20 compounding.But sliver stone
Under intermediate concentration consumption, the effect (promoting breast analysis) of drop flocculation increase particle effective volume is more than raising continuous phase for water shield polysaccharide
The effect (suppressing breast analysis) of viscosity, so as to cause system breast analysis unstable.Therefore preparation method of the present invention selects sliver
Sea lettuce polysaccharide, as emulsifying agent, is obtained good emulsifying effectiveness with polysorbas20 compound.
(3) content of the invention
It is an object of the invention to provide one kind prepares O/W types with sliver sea lettuce polysaccharide and polysorbas20 compound as emulsifying agent
The method of beta carotene emulsion, the method is easy to operate, low cost, and the beta carotene emulsion biology prepared using the method
Utilization rate height, good stability, biological safety are high.
For achieving the above object, the technical solution used in the present invention is：
A kind of sliver sea lettuce polysaccharide-polysorbas20 compounds the preparation method of beta carotene emulsion, comprises the steps：
(1) sliver sea lettuce polysaccharide and polysorbas20 are taken according to mass ratio 3:2 are dispersed in water, and obtain mass concentration for 3%-
4% sliver sea lettuce polysaccharide-polysorbas20 combination solution, i.e. water phase；
(2) beta carotene is dissolved in edible vegetable oil and obtains oil phase, the weight/mass percentage composition of wherein beta carotene is
0.3-0.5%, maintains the temperature at less than 60 DEG C in course of dissolution, to avoid temperature from producing unfavorable shadow to beta carotene stability
Ring；
(3) by water obtained by the step (2) gained oil phase and step (1) according to mass ratio 1：10～20 mixing are cut
Cut emulsification, then carry out high-pressure homogeneous, whole homogenizing process is carried out under ice-water bath, prevents Oxidation of Fat and Oils, obtains beta carotene
Emulsion.
Further, at least one of the edible vegetable oil in soybean oil, maize germ oil and sunflower oil.
Further, the weight/mass percentage composition of beta carotene is 0.5%.
Further, the speed of emulsification pretreatment is 10000-26000r/min, and preferably 26000r/min, the time is 3-
5min, preferably 3min.
Further, high-pressure homogeneous pressure is 50-75MPa, and preferably 75MPa, cycle-index is 1-3 time, preferably 3 times.
The preferably preparation method of the invention is carried out in accordance with the following steps：
(1) sliver sea lettuce polysaccharide and polysorbas20 are taken according to mass ratio 3:2 are dispersed in water, and obtain mass concentration for 3%-
4% sliver sea lettuce polysaccharide-polysorbas20 combination solution, i.e. water phase；
(2) beta carotene is dissolved in edible vegetable oil and obtains oil phase, the weight/mass percentage composition of wherein beta carotene is
0.3-0.5%, maintains the temperature at less than 60 DEG C in course of dissolution；
(3) by water obtained by the step (2) gained oil phase and step (1) according to mass ratio 1：10～20 mixing are cut
Cut emulsification, the speed of emulsification pretreatment is 10000-26000r/min, and the time is 3-5min, then carry out it is high-pressure homogeneous, it is high-pressure homogeneous
Pressure is 50-75MPa, and cycle-index is 1-3 time, and whole homogenizing process is carried out under ice-water bath, obtains beta carotene breast
Liquid.
The beneficial effects are mainly as follows：
(1) it is characteristic of the invention that using sliver sea lettuce polysaccharide-polysorbas20 compound as beta carotene emulsion emulsification
Agent, obtained stability of emulsion is high, and (in Fig. 2, sliver sea lettuce polysaccharide-polysorbas20 compounds beta carotene emulsion, after accelerated test
Its average grain diameter D [3,2] is 1.38 μm, hence it is evident that less than 2.04 μm of same concentration sliver sea lettuce polysaccharide beta carotene emulsion).This
It is because that polysorbas20 molecular structure is compact, sterically hindered effect is little, and the emulsification oxidant layer of densification is easily formed in oil-water interfaces, so as to
It is rapid to reduce interfacial tension, promote short grained formation；And sliver sea lettuce polysaccharide is polyhydroxy natural macromolecular, with certain
Molecular polarity, can be combined with each other with oil droplets in the form of Van der Waals force, and in droplet surface good hydrophilic colloid is formed
Film, prevents the polymerization between oil droplet, so as to increase the emulsifying capacity and stability of system.
(2) laurate in polysorbas20 has the ability of certain dissolving beta carotene so as to reduce external influence effect
Really, (in table 1, after 4 DEG C and 25 DEG C are stored 30 days, the beta carotene retention rate of two kinds of emulsions is poor to improve beta carotene retention rate
It is not especially big；But after 55 DEG C are stored 30 days, sliver sea lettuce polysaccharide-polysorbas20 compounding beta carotene emulsion beta carotene is protected
Rate is stayed to be 72%, and 55%) sliver sea lettuce polysaccharide beta carotene emulsion is only.
(3) in whole technical process of the invention, relatively low temperature is employed, beta carotene in food can be effectively improved
Biological utilisation effect (in Fig. 3, sliver sea lettuce polysaccharide-polysorbas20 compounding beta carotene emulsion bioavailability is 48.7%,
Sliver sea lettuce polysaccharide beta carotene emulsion is 37.8%, and biological dissolved with the oil phase with the beta carotene of same ratio in emulsion
Utilization rate is only 4.44%), to help absorption of the human body to beta carotene, and solve that beta carotene is difficult to be absorbed by the body asks
Topic.
(4) the emulsifying agent abundance used in the present invention, biodegradable, security；Emulsion preparation method is simple, behaviour
Facilitate, it is adaptable to industrialized production.
(4) illustrate
Fig. 1 is the preparation technology flow chart that the sliver sea lettuce polysaccharide-polysorbas20 of the present invention compounds beta carotene emulsion.
Fig. 2 is the sliver sea lettuce polysaccharide-polysorbas20 compounding beta carotene emulsion and sliver sea lettuce polysaccharide β-Hu Luo of the present invention
Bu Su stability of emulsion contrast (60 DEG C are placed 3d, 5d, 7d).
Fig. 3 is the sliver sea lettuce polysaccharide-polysorbas20 compounding beta carotene emulsion and sliver sea lettuce polysaccharide β-Hu Luo of the present invention
The beta carotene utilization rate contrast of Bu Su emulsions is (dissolved with right as blank with the oil phase of the beta carotene of same ratio in emulsion
According to).
(5) specific embodiment
Below technical scheme is described further with specific embodiment, but protection scope of the present invention is not limited
In this：
In the embodiment of the present invention, polysorbas20, soybean oil, beta carotene are purchased from Aladdin (aladdin) Reagent Company；
Sliver sea lettuce polysaccharide is picked up by oneself using Hot water extraction, and sliver sea lettuce takes from Wenzhou District of Zhejiang Province Pingyang County southern foot archipelago.
Embodiment 1
(1) sliver sea lettuce Polyose extraction：A certain amount of sliver sea lettuce is cleaned, water is controlled, 60 DEG C of drying in baking oven are placed in, is cut
It is broken.Plus 95% ethanol solution submergence degreasing, stand overnight.Sliver sea lettuce after degreasing is taken out and is dried, by 1：10 solid-liquid ratios add
Enter distilled water, 95 DEG C of water-bath 3h repeat to extract 3 times.Hot water is extracted and terminates rear cooling and standings, refrigerated centrifuge.Take supernatant rotation
It is concentrated by evaporation to certain volume.Add 95% ethanol solution to be 80% to final volume fraction of ethanol, stand overnight, 8000r/
Min low-temperature centrifugations, take precipitation, add a small amount of distillation water dissolves, and rotary evaporation will remain alcohol evaporating completely, and be concentrated into certain
Volume, with the de- albumen of Sevage methods, repeats 5 times, and rotary evaporation is to organic reagent evaporating completely.Freeze-drying obtains sliver stone
Water shield polysaccharide.
(2) prepared by beta carotene emulsion：Configuration sliver sea lettuce polysaccharide-polysorbas20 composite emulsifier, concentration is 3%, takes and splits
Piece sea lettuce polysaccharide and polysorbas20 are according to mass ratio 3:2 are dispersed in water, and stir 20min, stand 4h, obtain water phase；Again by β-Hu
Radish element is dissolved in soybean oil and obtains oil phase, and concentration is 0.5%.Under the stirring of high-speed shearing machine, according to oil phase and water phase quality
For 1：20 ratio, is slowly added to oil phase in water phase, 26000r/min shearing 3min, forms coarse emulsion.Coarse emulsion
By the further homogeneous of high pressure homogenizer 75MPa, circulate 3 times, obtain sliver sea lettuce polysaccharide-polysorbas20 compounding beta carotene breast
Liquid.
Embodiment 2
(1) sliver sea lettuce Polyose extraction：A certain amount of sliver sea lettuce is cleaned, water is controlled, 60 DEG C of drying in baking oven are placed in, is cut
It is broken.Plus 95% ethanol solution submergence degreasing, stand overnight.Sliver sea lettuce after degreasing is taken out and is dried, by 1：10 solid-liquid ratios add
Enter distilled water, 95 DEG C of water-bath 3h repeat to extract 3 times.Hot water is extracted and terminates rear cooling and standings, refrigerated centrifuge.Take supernatant rotation
It is concentrated by evaporation to certain volume.Add 95% ethanol solution to be 80% to final volume fraction of ethanol, stand overnight, 8000r/
Min low-temperature centrifugations, take precipitation, add a small amount of distillation water dissolves, and rotary evaporation will remain alcohol evaporating completely, and be concentrated into certain
Volume, with the de- albumen of Sevage methods, repeats 5 times, and rotary evaporation is to organic reagent evaporating completely.Freeze-drying obtains sliver stone
Water shield polysaccharide.
(2) prepared by beta carotene emulsion：Configuration sliver sea lettuce polysaccharide-polysorbas20 composite emulsifier, concentration is 4%, takes and splits
Piece sea lettuce polysaccharide and polysorbas20 are according to mass ratio 3:2 are dispersed in water, and stir 20min, stand 4h, obtain water phase；Again by β-Hu
Radish element is dissolved in soybean oil and obtains oil phase, and concentration is 0.3%.Under the stirring of high-speed shearing machine, according to oil phase and water phase quality
For 1：10 ratio, is slowly added to oil phase in water phase, 10000r/min shearing 5min, forms coarse emulsion.Coarse emulsion
By the further homogeneous of high pressure homogenizer 50MPa, circulate 3 times, obtain sliver sea lettuce polysaccharide-polysorbas20 compounding beta carotene breast
Liquid.
Comparative example 1
Using single sliver sea lettuce polysaccharide as emulsifying agent, emulsifier concentration in water phase is set to be 3%, other preparation processes are same
Embodiment 1, obtains sliver sea lettuce polysaccharide beta carotene emulsion.
Comparative example 2
Using single polysorbas20 as emulsifying agent, emulsifier concentration in water phase is set to be 3%, the same embodiment of other preparation processes
1, obtain polysorbas20 beta carotene emulsion.
Performance detection：
Stability of emulsion is determined：By sliver sea lettuce polysaccharide beta carotene emulsion, the polysorbas20 beta carotene of above-mentioned preparation
Emulsion and sliver sea lettuce polysaccharide-polysorbas20 compound storage 3d, 5d, 7d at beta carotene emulsion is placed in 60 DEG C and carry out accelerated test,
Determine its change of size and store at 4 DEG C, 25 DEG C, 55 DEG C respectively and determine after 30d beta carotene retention rate.As a result see Fig. 2 and
Table 1.
Table 1
Beta carotene bioavailability is determined：For above-mentioned emulsion, external gastro-intestinal Fluid digestion is simulated, will be through simulating intestines
Sample after digestion 2h is transferred in centrifuge tube, and ultracentrifugation is carried out at 4 DEG C, and centrifugal force is 10000g, and the time is
40min.Sample after centrifugation is generally divided into three layers, and the superiors are the cream layers that indigested oil phase is formed；It is loaded with nutrient
Transparent micella layer at middle one layer；It is fine and close insoluble that indigested cholate, protein, starch and free fatty are formed
Thing is then by centrifugation to orlop.The content of beta carotene in micella layer is determined, emulsion Jing is calculated by formula 1 and is disappeared
Bioavailability after change.In here experiment, adopt dissolved with the oil phase with the beta carotene of same ratio in emulsion as blank
Control.As a result Fig. 3 is seen.
Claims (8)
1. a kind of sliver sea lettuce polysaccharide-polysorbas20 compounds the preparation method of beta carotene emulsion, comprises the steps：
(1) sliver sea lettuce polysaccharide and polysorbas20 are taken according to mass ratio 3:2 are dispersed in water, and obtain mass concentration for 3%-4%'s
Sliver sea lettuce polysaccharide-polysorbas20 combination solution, i.e. water phase；
(2) beta carotene is dissolved in edible vegetable oil and obtains oil phase, wherein the weight/mass percentage composition of beta carotene is 0.3-
0.5%, less than 60 DEG C are maintained the temperature in course of dissolution；
(3) by water obtained by the step (2) gained oil phase and step (1) according to mass ratio 1：10～20 mixing carry out shearing breast
Change, then carry out high-pressure homogeneous, whole homogenizing process is carried out under ice-water bath, obtains beta carotene emulsion.
2. sliver sea lettuce polysaccharide as claimed in claim 1-polysorbas20 compounds the preparation method of beta carotene emulsion, its feature
It is：At least one of the edible vegetable oil in soybean oil, maize germ oil and sunflower oil.
3. sliver sea lettuce polysaccharide as claimed in claim 1 or 2-polysorbas20 compounds the preparation method of beta carotene emulsion, and it is special
Levy and be：In step (2), the weight/mass percentage composition of beta carotene is 0.5%.
4. sliver sea lettuce polysaccharide as claimed in claim 1 or 2-polysorbas20 compounds the preparation method of beta carotene emulsion, and it is special
Levy and be：The speed of emulsification pretreatment is 10000-26000r/min, and the time is 3-5min.
5. sliver sea lettuce polysaccharide as claimed in claim 4-polysorbas20 compounds the preparation method of beta carotene emulsion, its feature
It is：The speed of emulsification pretreatment is 26000r/min, and the time is 3min.
6. sliver sea lettuce polysaccharide as claimed in claim 1 or 2-polysorbas20 compounds the preparation method of beta carotene emulsion, and it is special
Levy and be：High-pressure homogeneous pressure is 50-75MPa, and cycle-index is 1-3 time.
7. sliver sea lettuce polysaccharide as claimed in claim 6-polysorbas20 compounds the preparation method of beta carotene emulsion, its feature
It is：High-pressure homogeneous pressure is 75MPa, and cycle-index is 3 times.
8. sliver sea lettuce polysaccharide as claimed in claim 1 or 2-polysorbas20 compounds the preparation method of beta carotene emulsion, and it is special
Levy is that the preparation method is carried out in accordance with the following steps：
(1) sliver sea lettuce polysaccharide and polysorbas20 are taken according to mass ratio 3:2 are dispersed in water, and obtain mass concentration for 3%-4%'s
Sliver sea lettuce polysaccharide-polysorbas20 combination solution, i.e. water phase；
(2) beta carotene is dissolved in edible vegetable oil and obtains oil phase, wherein the weight/mass percentage composition of beta carotene is 0.3-
0.5%, less than 60 DEG C are maintained the temperature in course of dissolution；
(3) by water obtained by the step (2) gained oil phase and step (1) according to mass ratio 1：10～20 mixing carry out shearing breast
Change, the speed of emulsification pretreatment is 10000-26000r/min, and the time is 3-5min, then carries out high-pressure homogeneous, high-pressure homogeneous pressure
For 50-75MPa, cycle-index is 1-3 time, and whole homogenizing process is carried out under ice-water bath, obtains beta carotene emulsion.
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| CN108041594A (en) * | 2017-11-28 | 2018-05-18 | 大连工业大学 | The preparation method of Patinopecten yessoensis Male reproduction zymolyte-beta carotene lotion |
| CN109601994A (en) * | 2018-09-06 | 2019-04-12 | 大连工业大学 | A kind of preparation method of scallop protein isolate-beta carotene lotion |
| CN111011858A (en) * | 2020-01-08 | 2020-04-17 | 中国农业科学院农产品加工研究所 | Mixed oil emulsion for improving bioavailability of fat-soluble active substances and preparation method thereof |
| WO2022079063A3 (en) * | 2020-10-13 | 2022-05-27 | Givaudan Sa | Composition |
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| CN108041594A (en) * | 2017-11-28 | 2018-05-18 | 大连工业大学 | The preparation method of Patinopecten yessoensis Male reproduction zymolyte-beta carotene lotion |
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| CN111011858A (en) * | 2020-01-08 | 2020-04-17 | 中国农业科学院农产品加工研究所 | Mixed oil emulsion for improving bioavailability of fat-soluble active substances and preparation method thereof |
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