CN106720462B - A kind of linseed oil emulsion and preparation method thereof for making emulsifier with flax glue compounding - Google Patents
A kind of linseed oil emulsion and preparation method thereof for making emulsifier with flax glue compounding Download PDFInfo
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- CN106720462B CN106720462B CN201611034187.2A CN201611034187A CN106720462B CN 106720462 B CN106720462 B CN 106720462B CN 201611034187 A CN201611034187 A CN 201611034187A CN 106720462 B CN106720462 B CN 106720462B
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23D—EDIBLE OILS OR FATS, e.g. MARGARINES, SHORTENINGS, COOKING OILS
- A23D9/00—Other edible oils or fats, e.g. shortenings, cooking oils
- A23D9/007—Other edible oils or fats, e.g. shortenings, cooking oils characterised by ingredients other than fatty acid triglycerides
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23D—EDIBLE OILS OR FATS, e.g. MARGARINES, SHORTENINGS, COOKING OILS
- A23D9/00—Other edible oils or fats, e.g. shortenings, cooking oils
- A23D9/02—Other edible oils or fats, e.g. shortenings, cooking oils characterised by the production or working-up
- A23D9/04—Working-up
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- Life Sciences & Earth Sciences (AREA)
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- Food Science & Technology (AREA)
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- Emulsifying, Dispersing, Foam-Producing Or Wetting Agents (AREA)
- General Preparation And Processing Of Foods (AREA)
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Abstract
The invention discloses a kind of linseed oil emulsion and preparation method thereof for making emulsifier with flax glue compounding.The preparation method of the linseed oil emulsion includes the following steps: 1) to prepare the aqueous solution of emulsifier as water phase;Emulsifier is the mixture of Arabic gum and flax glue;2) using linseed oil as oily phase, oil is added into water phase, colostric fluid is obtained;3) colostric fluid is through homogeneous to get linseed oil emulsion.Linseed oil emulsion particle diameter of the present invention is small, good dispersion and physical stability are high, there is higher oxidation stability under the conditions of different temperature and pH simultaneously, and compare the emulsion that protein-polysaccharide is prepared layer by layer and have the stronger ability for resisting environmental change, preferable stability can be kept in the process such as wider pH range, larger ionic strength and sterilizing, freeze thawing.Linseed oil emulsion storage-stable prepared by the present invention is good, and can play preferable protective effect to linolenic acid compared to the pure oil systems of linseed oil.
Description
Technical field
The present invention relates to a kind of linseed oil emulsion and preparation method thereof for making emulsifier with flax glue compounding, belong to oil
Rouge manufacture field.
Background technique
Linseed oil is a kind of high unsaturated fatty acid (80% or more) vegetable oil, and wherein alpha-linolenic acid content is up to 60%
More than.Alpha-linolenic acid is essential fatty acid, metabolizable in human body to be converted to EPA and DHA, these three fatty acid belong to
Omega-fatty acid can promote the development of infant's brain and nervous system, improve memory, especially to reduction cardiovascular and cerebrovascular disease
The onset risk of disease has certain effect.China's resident's edible oil is mostly with soybean oil, peanut oil, sunflower oil and the cottonseed after refining
Based on oil etc., saturated fatty acid and the intake of ω -6 unsaturated fatty acid are more, and omega-fatty acid intake is very few.According to FAO/
The suggestion of WHO 2008, ω -6 and omega-fatty acid absorption ratio should be 5:1 in human diet.Linseed oil is rich in α-flax
Acid can be used as the main edible source of omega-fatty acid.International fatty acid and grease research association (ISSFAL) suggest taking the photograph daily
Enter alpha-linolenic acid 2.2g.
However, alpha-linolenic acid contains three unsaturated bonds, and it is extremely unstable, it is easily lost during conventional culinary, and
Toxic hydroperoxides and smell substance can be generated, seriously limit linseed oil in diet and food processing formula
Application.If direct edible oil and fat, the eating habit traditional to China resident challenge is larger again, it is difficult to carry out.Emulsion tool
There is continuous sliding mouthfeel, linseed oil is prepared into stable emulsion, on the one hand greatly remains distinctive functionality in oil
On the other hand ingredient has adapted to the traditional diet of China resident, to solve efficiently to supplement omega-3 unsaturated fatty acid
Problem.Mayonnaise in the highly viscous alternative fruit and vegetable salad of emulsion, plays omega-fatty acid in diet
Invigoration effect, low viscosity emulsion can be used as beverage, it is easier to daily carrying and drink.
Preparing the common emulsifier of oil-in-water emulsion at present is protein and polysaccharide, and protein emulsibility is preferable, but cream
Shape liquid interfacial film thinner thickness, it is easily affected by environment, oil droplet coalescence can be caused when system pH is near isoelectric point, causes body
It is unstable.Therefore it needs to provide a kind of preparation method of new linseed oil emulsion.
Summary of the invention
The object of the present invention is to provide a kind of linseed oil emulsion and its preparation sides for making emulsifier with flax glue compounding
Method, one aspect of the present invention greatly remain distinctive functional components in linseed oil, have on the other hand adapted to China resident
Traditional diet, to solve the problems, such as efficiently to supplement omega-3 unsaturated fatty acid.
The preparation method of linseed oil emulsion provided by the invention, includes the following steps:
1) aqueous solution of emulsifier is prepared as water phase;
The emulsifier is the mixture of Arabic gum and flax glue;
2) using linseed oil as oily phase, the oil is added into the water phase, colostric fluid is obtained;
3) colostric fluid is through homogeneous to get the linseed oil emulsion.
In above-mentioned preparation method, in step 1), in the emulsifier, the quality of the Arabic gum and the flax glue
Than that can be 1~4:1, concretely 2~3:1,2:1,2.3:1 or 3:1.
The molecular weight of the Arabic gum can be 20~600,000;
The molecular weight of the flax glue can be 260~3,500,000, and the flax glue is a kind of natural polymer compound adhesive, main
It is distributed in the kind skin of linseed, a certain amount of protein is combined in polysaccharide molecule structure, there is emulsibility and emulsification
Stability.
In above-mentioned preparation method, in step 1), before the aqueous solution for preparing the emulsifier, the method also includes will
The step of emulsifier is mixed with glycerol;
The mass ratio of the emulsifier and the glycerol can be 1~3:1, concretely 1.5~3:1,1.5:1,2:1 or 3:
1, effect is to reduce interfacial tension, makes interface be easier to flow, to reduce bending energy needed for droplet formation, helps to stablize
The formation of lotion.
In above-mentioned preparation method, in step 1), in the aqueous solution of the emulsifier, the quality percentage of the emulsifier contains
Amount can be 1.5~2.5%, concretely 1.5~2.0%, 2.0~2.5%, 1.5%, 2.0% or 2.5%.
In above-mentioned preparation method, the method also includes the water phase to be swollen, to guarantee abundant water
It closes.
In above-mentioned preparation method, the step of swelling, is as follows: under conditions of 50 DEG C~60 DEG C stirring swelling 1~
Then 2h is swollen 10~14h under conditions of 20 DEG C~25 DEG C, such as: stirring swelling 1h under conditions of 60 DEG C, then at 25 DEG C
Under conditions of be swollen 10h.
In above-mentioned preparation method, in step 2), the oil can be mutually 1:2~4 with the volume ratio of the water phase, specifically may be used
For 1:2,1:3 or 1:4.
In above-mentioned preparation method, in step 2), the oil is added into the water phase under conditions of shearing;
The condition of the shearing is as follows:
Rate can be 10000~20000rpm, concretely 10000rpm, 15000rpm or 20000rpm;
Time can be 2~4min, concretely 2min.
In above-mentioned preparation method, in step 3), the homogeneous is by the way of high-pressure homogeneous;
The homogeneous homogeneous includes level-one homogeneous and double-stage homogenization;
The pressure of the level-one homogeneous can be 250~350bar, concretely 250bar, 300bar or 350bar;
The pressure of the double-stage homogenization can be 40~80bar, concretely 50bar.
The linseed oil emulsion that the above method is prepared also belongs to protection scope of the present invention.
The invention has the following beneficial effects:
1, the emulsifier that uses of the present invention is for Arabic gum and the flax glue homologous with linseed oil, for a kind of naturally green
Color emulsifier, edible safety is high to linseed availability, without emulsifiers such as Tween, Span.
2, prepared by the present invention linseed oil emulsion particle diameter is small, good dispersion and physical stability are high, while not
With temperature and pH under the conditions of have higher oxidation stability, and compare the emulsion that protein-polysaccharide is prepared layer by layer have it is stronger
Resistance environmental change ability, can be kept in the process such as wider pH range, larger ionic strength and sterilizing, freeze thawing
Preferable stability.
3, the linseed oil emulsion storage-stable prepared by the present invention is good, and compared to the pure oil systems pair of linseed oil
Linolenic acid can play preferable protective effect.
Detailed description of the invention
Fig. 1 is the backscattered light scanning figure of linseed oil emulsion prepared by the embodiment of the present invention 1.
Specific embodiment
Experimental method used in following embodiments is conventional method unless otherwise specified.
The materials, reagents and the like used in the following examples is commercially available unless otherwise specified.
Embodiment 1 prepares linseed oil emulsion
1) mixture of Arabic gum (molecular weight is 20~600,000) and flax glue (molecular weight is 260~3,500,000) is taken to make
For emulsifier (mass ratio 7:3), glycerol is added and drenches (amount ratio of emulsifier and glycerol is 2:1), into the material mixed
Sterile pure water is added, is configured to the aqueous solution of 2.5wt% emulsifier, (temperature is about for swelling overnight after magnetic agitation 1h at 60 DEG C
25 DEG C, 10h), guarantee sufficiently hydration.
2) using linseed oil as oily phase, according to the oil-water ratio of 1:4 (v/v), in shear rate 10000rpm and shearing
Oil is mutually slowly added among water phase in time 2min, forms colostric fluid.
3) double-stage homogenization is carried out using high pressure homogenizer, level-one homogenization pressure is 350bar, and double-stage homogenization pressure is
50bar prepares stable linseed oil emulsion.
The partial size of linseed oil emulsion manufactured in the present embodiment is 2.8 μm.
The backscattered light scanning figure (stability analysis tester (AGS)) of linseed oil emulsion manufactured in the present embodiment is such as
Shown in Fig. 1, in Fig. 1, left side representative sample bottom of pond portion, right side represents top, and ordinate is backscattered light intensity value, sweeps for the first time
It retouches and is displayed in blue, be red, the higher proof more stable emulsion of the two registration, it can be seen that the present embodiment system for the last time
Standby linseed oil emulsion particle diameter variation is small, and substantially without upper floating layer, emulsion is highly stable.
The storage-stable experiment of linseed oil emulsion manufactured in the present embodiment is as follows:
Linolenic acid content reduces respectively after linseed oil emulsion of the present invention is stored 20 days respectively under the conditions of 4 DEG C and 25 DEG C
10.87% and 12.25%;And linolenic acid content reduces respectively after linseed oil is stored 20 days respectively under the conditions of 4 DEG C and 25 DEG C
15.53% and 18.94%, it is seen that the two have significant difference (P < 0.05), it was demonstrated that linseed oil emulsion of the present invention compared to
The pure oil systems of linseed oil can play preferable protective effect to linolenic acid.
The test experiments of the ability of the resistance environmental change of linseed oil emulsion manufactured in the present embodiment are as follows:
It compares linseed oil emulsion prepared by this example and is compounded with soybean protein-Arabic gum and make emulsifier preparation
Linseed oil emulsion (preparation method is identical as the present embodiment, the difference is that replacing flax glue with soybean protein) is in difference
Under pH condition (3~9), under different ionic strength condition (sodium chloride solution and calcium chloride solution of 0.1~0.5mol/L), and
Sterilizing (121 DEG C of sterilizing 15min in high-pressure sterilizing pot) and freeze-thaw (are freezed for 24 hours under the conditions of -20 DEG C, are then put in room temperature
Set 12h as one recycle, altogether recycle 3 times) experiment under stability, the results showed that protein-polysaccharide emulsion due to
Emulsifier contains protein, is still vulnerable to pH influence, wild effect occurs near isoelectric point.Hydrophilic colloid preparation of the present invention
Emulsion, which compares it, the stronger ability for resisting environmental change, can be in wider pH range, larger ionic strength and sterilizing, jelly
Melt etc. in process and keeps preferable stability.
Embodiment 2 prepares linseed oil emulsion
1) mixture of Arabic gum (molecular weight is 20~600,000) and flax glue (molecular weight is 260~3,500,000) is taken to make
For emulsifier (mass ratio 2:1), glycerol is added and drenches (amount ratio of emulsifier and glycerol is 3:2), into the material mixed
Sterile pure water is added, is configured to the aqueous solution of 1.5wt% emulsifier, (temperature is about for swelling overnight after magnetic agitation 1h at 60 DEG C
25 DEG C, 10h), guarantee sufficiently hydration.
2) using linseed oil as oily phase, according to the oil-water ratio of 1:2 (v/v), in shear rate 20000rpm and shearing
Oil is mutually slowly added among water phase in time 2min, forms colostric fluid.
3) double-stage homogenization is carried out using high pressure homogenizer, level-one homogenization pressure is 250bar, and double-stage homogenization pressure is
50bar prepares stable linseed oil emulsion.
The partial size of linseed oil emulsion manufactured in the present embodiment is 3.1 μm.
The backscattered light scanning figure and Fig. 1 of linseed oil emulsion manufactured in the present embodiment are without substantive difference, it was demonstrated that this
Embodiment linseed oil emulsion has good physical stability.
The storage-stable experiment of linseed oil emulsion manufactured in the present embodiment is as follows:
Linolenic acid content reduces respectively after linseed oil emulsion of the present invention is stored 20 days respectively under the conditions of 4 DEG C and 25 DEG C
11.25% and 13.14%;And linolenic acid content reduces respectively after linseed oil is stored 20 days respectively under the conditions of 4 DEG C and 25 DEG C
15.53% and 18.94%, it is seen that the two have significant difference (P < 0.05), it was demonstrated that linseed oil emulsion of the present invention compared to
The pure oil systems of linseed oil can play preferable protective effect to linolenic acid.
It compared linseed oil emulsion manufactured in the present embodiment and compound with soybean protein-Arabic gum to make emulsifier preparation
Linseed oil emulsion resist environmental change ability, the experimental results showed that hydrophilic colloid of the present invention prepare emulsion compare
It has the stronger ability for resisting environmental change, can be at the techniques such as wider pH range, larger ionic strength and sterilizing, freeze thawing
Preferable stability is kept in reason.
Embodiment 3 prepares linseed oil emulsion
1) mixture of Arabic gum (molecular weight is 20~600,000) and flax glue (molecular weight is 260~3,500,000) is taken to make
For emulsifier (mass ratio 3:1), glycerol is added and drenches (amount ratio of emulsifier and glycerol is 3:1), into the material mixed
Sterile pure water is added, is configured to the aqueous solution of 2wt% emulsifier, (temperature is about 25 for swelling overnight after magnetic agitation 1h at 60 DEG C
DEG C, 10h), guarantee sufficiently hydration.
2) using linseed oil as oily phase, according to the oil-water ratio of 1:3 (v/v), in shear rate 15000rpm and shearing
Oil is mutually slowly added among water phase in time 2min, forms colostric fluid.
3) double-stage homogenization is carried out using high pressure homogenizer, level-one homogenization pressure is 300bar, and double-stage homogenization pressure is
50bar prepares stable linseed oil emulsion.
The partial size of linseed oil emulsion manufactured in the present embodiment is 3.0 μm.
The backscattered light scanning figure and Fig. 1 of linseed oil emulsion manufactured in the present embodiment are without substantive difference, it was demonstrated that this
Embodiment linseed oil emulsion has good physical stability.
The storage-stable experiment of linseed oil emulsion manufactured in the present embodiment is as follows:
Linolenic acid content reduces respectively after linseed oil emulsion of the present invention is stored 20 days respectively under the conditions of 4 DEG C and 25 DEG C
11.05% and 12.74%;And linolenic acid content reduces respectively after linseed oil is stored 20 days respectively under the conditions of 4 DEG C and 25 DEG C
15.53% and 18.94%, it is seen that the two have significant difference (P < 0.05), it was demonstrated that linseed oil emulsion of the present invention compared to
The pure oil systems of linseed oil can play preferable protective effect to linolenic acid
It compared linseed oil emulsion manufactured in the present embodiment and compound with soybean protein-Arabic gum to make emulsifier preparation
Linseed oil emulsion resist environmental change ability, the experimental results showed that hydrophilic colloid of the present invention prepare emulsion compare
It has the stronger ability for resisting environmental change, can be at the techniques such as wider pH range, larger ionic strength and sterilizing, freeze thawing
Preferable stability is kept in reason.
Claims (6)
1. a kind of preparation method of linseed oil emulsion, includes the following steps:
1) aqueous solution of emulsifier is prepared as water phase;
The emulsifier is the mixture of Arabic gum and flax glue, the mass ratio of the Arabic gum and the flax glue is 1 ~
4:1;
The molecular weight of the Arabic gum is 20 ~ 600,000;
The molecular weight of the flax glue is 260 ~ 3,500,000;
In the aqueous solution of the emulsifier, the mass percentage of the emulsifier is 1.5 ~ 2.5%;
2) using linseed oil as oily phase, the oil is added into the water phase, colostric fluid is obtained;
The oil is mutually 1:2 ~ 4 with the volume ratio of the water phase;
3) colostric fluid is through homogeneous to get the linseed oil emulsion;
The homogeneous includes level-one homogeneous and double-stage homogenization;
The pressure of the level-one homogeneous is 250 ~ 350 bar;
The pressure of the double-stage homogenization is 40 ~ 80 bar.
2. preparation method according to claim 1, it is characterised in that: in step 1), prepare the aqueous solution of the emulsifier
Before, the method also includes mixing the emulsifier with glycerol;
The mass ratio of the emulsifier and the glycerol is 1 ~ 3:1.
3. preparation method according to claim 1 or 2, it is characterised in that: the method also includes carrying out the water phase
The step of swelling.
4. preparation method according to claim 3, it is characterised in that: the step of swelling is as follows: at 50 DEG C ~ 60 DEG C
Under the conditions of stirring swelling 1 ~ 2h, then under conditions of 20 DEG C ~ 25 DEG C be swollen 10 ~ 14h.
5. preparation method according to claim 1 or 2, it is characterised in that:, will be described under conditions of shearing in step 2
Oil is added into the water phase;
The condition of the shearing is as follows:
Rate is 10000 ~ 20000 rpm;
Time is 2 ~ 4 min.
6. the linseed oil emulsion of any one of claim 1-5 the method preparation.
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CN108968079A (en) * | 2018-07-16 | 2018-12-11 | 南京财经大学 | A kind of edibility rapeseed protein lotion and preparation method thereof |
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CN109938328B (en) * | 2019-04-03 | 2022-04-29 | 东北农业大学 | Method for preparing fat substitute by regulating and controlling oil drop aggregation in emulsion through flaxseed gum |
CN110090181A (en) * | 2019-04-16 | 2019-08-06 | 上海悦目化妆品有限公司 | A kind of preparation method and applications of linseed emulsifier |
CN110679915B (en) * | 2019-09-19 | 2022-11-18 | 郑州轻工业大学 | Preparation method of linseed protein/linseed gum/polyphenol complex coacervation embedding system |
CN112515169A (en) * | 2020-11-24 | 2021-03-19 | 中国农业大学 | Flaxseed oil, and preparation method of compound emulsion and high internal phase emulsion thereof |
CN114468294B (en) * | 2022-01-24 | 2024-04-02 | 河南农业大学 | Nanoemulsion simultaneously loaded with linseed oil and lutein and preparation method thereof |
CN115251359B (en) * | 2022-07-15 | 2024-03-15 | 中国农业科学院油料作物研究所 | Plant-based delivery system capable of covering bad flavor of grease, preparation method and application |
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