CN108030471B - Animal gastrointestinal motility detection system and detection method - Google Patents
Animal gastrointestinal motility detection system and detection method Download PDFInfo
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- CN108030471B CN108030471B CN201711383093.0A CN201711383093A CN108030471B CN 108030471 B CN108030471 B CN 108030471B CN 201711383093 A CN201711383093 A CN 201711383093A CN 108030471 B CN108030471 B CN 108030471B
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- 241001465754 Metazoa Species 0.000 title claims abstract description 42
- 238000001514 detection method Methods 0.000 title claims abstract description 32
- 230000005176 gastrointestinal motility Effects 0.000 title claims abstract description 31
- 239000003550 marker Substances 0.000 claims abstract description 99
- 210000003238 esophagus Anatomy 0.000 claims abstract description 29
- 210000001035 gastrointestinal tract Anatomy 0.000 claims abstract description 27
- 238000010171 animal model Methods 0.000 claims abstract description 25
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 claims abstract description 20
- 229910052742 iron Inorganic materials 0.000 claims abstract description 10
- 230000030136 gastric emptying Effects 0.000 claims abstract description 9
- 210000003608 fece Anatomy 0.000 claims abstract description 7
- NTHXOOBQLCIOLC-UHFFFAOYSA-N iohexol Chemical compound OCC(O)CN(C(=O)C)C1=C(I)C(C(=O)NCC(O)CO)=C(I)C(C(=O)NCC(O)CO)=C1I NTHXOOBQLCIOLC-UHFFFAOYSA-N 0.000 claims abstract description 6
- 229960001025 iohexol Drugs 0.000 claims abstract description 6
- 239000000203 mixture Substances 0.000 claims abstract description 5
- 206010002091 Anaesthesia Diseases 0.000 claims abstract description 4
- 230000037005 anaesthesia Effects 0.000 claims abstract description 4
- 229910052788 barium Inorganic materials 0.000 claims abstract description 4
- DSAJWYNOEDNPEQ-UHFFFAOYSA-N barium atom Chemical compound [Ba] DSAJWYNOEDNPEQ-UHFFFAOYSA-N 0.000 claims abstract description 4
- 238000000034 method Methods 0.000 claims description 20
- 210000002318 cardia Anatomy 0.000 claims description 8
- 210000001187 pylorus Anatomy 0.000 claims description 6
- 230000000747 cardiac effect Effects 0.000 claims description 4
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 3
- 210000000436 anus Anatomy 0.000 claims description 3
- 239000000741 silica gel Substances 0.000 claims description 3
- 229910002027 silica gel Inorganic materials 0.000 claims description 3
- 238000007599 discharging Methods 0.000 abstract 1
- 238000011084 recovery Methods 0.000 abstract 1
- 241000699670 Mus sp. Species 0.000 description 6
- 238000002474 experimental method Methods 0.000 description 5
- 241000699666 Mus <mouse, genus> Species 0.000 description 4
- 230000002496 gastric effect Effects 0.000 description 4
- 230000000384 rearing effect Effects 0.000 description 4
- 210000002784 stomach Anatomy 0.000 description 4
- 238000011161 development Methods 0.000 description 3
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 3
- 206010010774 Constipation Diseases 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 239000002872 contrast media Substances 0.000 description 2
- 230000007547 defect Effects 0.000 description 2
- 201000010099 disease Diseases 0.000 description 2
- 201000006549 dyspepsia Diseases 0.000 description 2
- 239000012530 fluid Substances 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 208000021302 gastroesophageal reflux disease Diseases 0.000 description 2
- 238000003780 insertion Methods 0.000 description 2
- 230000037431 insertion Effects 0.000 description 2
- 208000002551 irritable bowel syndrome Diseases 0.000 description 2
- 210000004400 mucous membrane Anatomy 0.000 description 2
- 230000002035 prolonged effect Effects 0.000 description 2
- 238000010186 staining Methods 0.000 description 2
- 235000004391 Chenopodium capitatum Nutrition 0.000 description 1
- 244000038022 Chenopodium capitatum Species 0.000 description 1
- 206010012735 Diarrhoea Diseases 0.000 description 1
- 208000018498 Gastrointestinal dyskinetic disease Diseases 0.000 description 1
- 206010047700 Vomiting Diseases 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000001647 drug administration Methods 0.000 description 1
- 238000002594 fluoroscopy Methods 0.000 description 1
- 244000144993 groups of animals Species 0.000 description 1
- 238000003384 imaging method Methods 0.000 description 1
- 238000001727 in vivo Methods 0.000 description 1
- 210000000936 intestine Anatomy 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 230000001575 pathological effect Effects 0.000 description 1
- 238000005086 pumping Methods 0.000 description 1
- 230000005855 radiation Effects 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 238000007447 staining method Methods 0.000 description 1
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- 239000002699 waste material Substances 0.000 description 1
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/42—Detecting, measuring or recording for evaluating the gastrointestinal, the endocrine or the exocrine systems
- A61B5/4222—Evaluating particular parts, e.g. particular organs
- A61B5/4238—Evaluating particular parts, e.g. particular organs stomach
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/42—Detecting, measuring or recording for evaluating the gastrointestinal, the endocrine or the exocrine systems
- A61B5/4222—Evaluating particular parts, e.g. particular organs
- A61B5/4255—Intestines, colon or appendix
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/68—Arrangements of detecting, measuring or recording means, e.g. sensors, in relation to patient
- A61B5/6846—Arrangements of detecting, measuring or recording means, e.g. sensors, in relation to patient specially adapted to be brought in contact with an internal body part, i.e. invasive
- A61B5/6867—Arrangements of detecting, measuring or recording means, e.g. sensors, in relation to patient specially adapted to be brought in contact with an internal body part, i.e. invasive specially adapted to be attached or implanted in a specific body part
- A61B5/687—Oesophagus
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/68—Arrangements of detecting, measuring or recording means, e.g. sensors, in relation to patient
- A61B5/6846—Arrangements of detecting, measuring or recording means, e.g. sensors, in relation to patient specially adapted to be brought in contact with an internal body part, i.e. invasive
- A61B5/6867—Arrangements of detecting, measuring or recording means, e.g. sensors, in relation to patient specially adapted to be brought in contact with an internal body part, i.e. invasive specially adapted to be attached or implanted in a specific body part
- A61B5/6871—Stomach
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61B—DIAGNOSIS; SURGERY; IDENTIFICATION
- A61B5/00—Measuring for diagnostic purposes; Identification of persons
- A61B5/68—Arrangements of detecting, measuring or recording means, e.g. sensors, in relation to patient
- A61B5/6846—Arrangements of detecting, measuring or recording means, e.g. sensors, in relation to patient specially adapted to be brought in contact with an internal body part, i.e. invasive
- A61B5/6867—Arrangements of detecting, measuring or recording means, e.g. sensors, in relation to patient specially adapted to be brought in contact with an internal body part, i.e. invasive specially adapted to be attached or implanted in a specific body part
- A61B5/6873—Intestine
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Heart & Thoracic Surgery (AREA)
- Medical Informatics (AREA)
- Physics & Mathematics (AREA)
- Veterinary Medicine (AREA)
- Biophysics (AREA)
- Pathology (AREA)
- Engineering & Computer Science (AREA)
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- Public Health (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Surgery (AREA)
- Animal Behavior & Ethology (AREA)
- Physiology (AREA)
- Endocrinology (AREA)
- Gastroenterology & Hepatology (AREA)
- Measuring And Recording Apparatus For Diagnosis (AREA)
- Apparatus For Radiation Diagnosis (AREA)
Abstract
The invention discloses an animal gastrointestinal motility detection system and a detection method, wherein the detection system comprises a marker and a marker grasping device; the marker is of a layered structure, and comprises a marker inner core, a marker inner shell and a marker outer shell in sequence from inside to outside, wherein the marker inner core is a mixture of iron or barium which is impermeable to X rays; the marker gripping device includes a gripping portion, a controller, and an elongated connecting portion connecting the two. The detection method comprises the steps of filling iohexol into the esophagus cavity of the experimental animal after anesthesia, releasing a marker at the upper end of the esophagus, dynamically observing the esophageal emptying function of the experimental animal under X-ray equipment, prolonging the observation time, and measuring the gastric emptying time; and (3) discharging the markers along with the feces after a certain time, performing X-ray examination after recovery to count the number of the residual markers in the feces, and calculating the emptying rate of the gastrointestinal tract in a certain time. The invention can continuously and dynamically observe the relevant detection indexes of the gastrointestinal motility of animals and has wide application prospect.
Description
Technical Field
The invention relates to the field of gastrointestinal motility detection methods, in particular to an animal gastrointestinal motility detection system and an animal gastrointestinal motility detection method.
Background
Gastrointestinal dysmotility disorders are very common, and the whole digestive tract can be affected almost, mainly involving gastroesophageal reflux disease (GERD), functional Dyspepsia (FD), irritable Bowel Syndrome (IBS), functional Constipation (FC), and the like. In recent years, with the rapid development of socioeconomic, the prevalence of gastrointestinal dyskinetic diseases has been on the rise year by year, and the disease seriously affects the quality of life of patients, and thus has been receiving increasing attention.
The current methods for detecting gastrointestinal motility function of experimental animals are various, and mainly comprise the following steps: 1. radionuclide imaging: the method is an in-vivo, noninvasive gastrointestinal motility detection method. After standard trials of oral administration of drugs containing radionuclide labels (99 mTc-SC or 111 In-DTPA), continuous photographs were taken In the examination area by gamma-camera, and gastrointestinal motility was evaluated based on the amount of food radionuclide In the stomach. The disadvantages of this method are mainly the high equipment requirements, the risk of nuclear radiation, and the high cost, the long time consumption, the poor animal compliance, which severely limit the application of this method. 2. Ink staining method: the method comprises the steps of feeding the animal with a certain concentration of Indian ink, killing the animal after a certain period of time, freeing the gastrointestinal tract, and measuring the distance from the stomach to the far end of the ink staining, thereby representing the gastrointestinal tract movement function of the animal. The method has the defects that an animal killing party can be used for measuring, only gastrointestinal tract movement function indexes at one time point can be detected, dynamic monitoring cannot be realized, if a plurality of time point movement function indexes are needed, a plurality of groups of animals are needed to be killed at different time points respectively, and the animal killing method can be used for detecting, so that a large amount of waste is caused, and mucous membrane is polluted due to ink staining, so that the method is not beneficial to pathological research of mucous membrane specimens. And 3, measuring the pressure in esophagus or colorectal of the experimental animal by using a special catheter for animal experiments, and indirectly reflecting the movement function of the digestive tract. However, the method is limited by the number of the catheter channels and the length of the catheter, cannot accurately and intuitively reflect the full digestive tract movement function, can only measure in a short time, cannot provide the digestive tract dynamic movement function data of long-term experimental animals, and in addition, the method needs pressure measuring equipment to provide support, so that the method is limited to be widely applied. The above method for detecting the animal digestive tract movement function can not detect the esophageal emptying function of the experimental animal except the defects.
With the development of society, domestic pets are becoming more and more popular. Many pet diseases are manifested by gastrointestinal symptoms such as diarrhea, constipation, vomiting and the like, and the current targeted examination means are not many, and the gastrointestinal motility function cannot be directly reflected; and poor drug administration pertinence is caused by lack of targeted gastrointestinal motility examination, which severely restricts the development of pet medical treatment and increases the household burden.
Disclosure of Invention
The invention aims to provide an animal gastrointestinal motility detection system and a detection method, which are used for dynamically, safely and accurately realizing the acquisition of the dynamic function indexes of the whole digestive tract (esophagus, stomach and intestines) and repeatedly measuring for multiple times so as to reduce the consumption of experimental animals.
The invention is realized according to the following technical scheme.
An animal gastrointestinal motility detection system comprises a marker and a marker grasping device; the marker is of a layered structure, and comprises a marker inner core, a marker inner shell and a marker outer shell in sequence from inside to outside, wherein the marker inner core is a mixture of iron or barium which is impermeable to X rays; the marker gripping device comprises a gripping part, a controller and an elongated connecting part for connecting the gripping part and the controller; the tail end of the gripping part forms an opening for accommodating the marker; the controller controls the gripping part to grip or release the marker.
Further, the inside of the gripping part and the connecting part is of a cavity structure; the tail end of the gripping part is internally provided with an attracting part with a through hole in the center, and the attracting part is a magnet and is attracted with the marker inner shell consisting of iron or a magnet; one end of the pushing rod penetrates into the through hole in the center of the suction part, and the other end of the pushing rod penetrates through the connecting part to be connected with the controller; a stepping motor is arranged in the controller, and a rotor of the stepping motor is connected with one end of the pushing rod.
Further, the inside of the gripping part and the connecting part is of a cavity structure; the tail end of the gripping part is internally provided with an attracting part with a through hole in the center, and the attracting part is a magnet and is attracted with the marker inner shell consisting of iron or a magnet; the controller controls the gripping part to grip or release the marker through air pressure or hydraulic pressure.
Furthermore, the marker shell is made of silica gel.
Further, the marker is spherical, ellipsoidal or short rod-shaped.
Further, a graduated scale is arranged on the outer wall of the connecting part.
Further, the magnet is a permanent magnet or an electromagnet.
A method for detecting gastrointestinal motility of an animal comprising the steps of:
a. after the animals are anesthetized, a certain amount of iohexol with a certain concentration is poured into the esophagus cavity of the experimental animal to outline the digestive tract;
b. releasing the markers from the upper end of the esophagus of the experimental animal through the mouth to enable the markers to be discharged to the anus side along with the movement of the digestive tract of the experimental animal, and acquiring relevant parameters in the process of removing the markers;
c. calculating esophageal movement function parameters: positioning esophageal entrance release marker as X 1 The cardiac orifice is positioned at X 2 The method comprises the steps of carrying out a first treatment on the surface of the Record X 1 To X 2 Distance L between the two points, and detecting the movement time T of the marker passing through the two points, and the esophageal evacuation speed: v=l/T (cm/s); speed of evacuation of a certain segment of the esophagus: the starting point of a certain section of the esophagus is defined as X m The length of the esophagus is L m =X 2 -X m Marker pass X m To pass through X 2 The time is recorded as T m The esophageal evacuation speed is L m /T m ；
d. Calculating gastric emptying parameters: recording the time from the entrance of the marker to the exit of the gastric cardia to the exit of the marker, wherein the length from the entrance of the marker to the exit of the gastric cardia is defined as L n The whole discharge time is recorded as T n Gastric emptying rate of L n /T n ；
e. Calculating gastrointestinal tract evacuation parameters: releasing a plurality of markers at the upper end of esophagus of the experimental animal, after the plurality of markers enter the cardia, removing anesthesia, raising normally, collecting the excreted feces of the experimental animal regularly, performing X-ray examination, counting the number of the markers in the feces, and performing gastrointestinal tract emptying rate in the time interval: (n) Row of rows /n Total (S) ）%。
The invention achieves the following beneficial effects.
The invention can continuously observe the gastrointestinal tract emptying condition of animals under the non-killing condition; the observation time is prolonged, and the data such as the emptying time, the emptying rate and the like of the whole digestive tract of the animal can be obtained; the invention can continuously and dynamically observe the relevant detection indexes of the gastrointestinal motility of animals, reduces the cost in the aspects of equipment, consumable materials and animal consumption, greatly improves the intuitiveness, continuity and accuracy of experimental results, and has important significance in the aspect of detecting the gastrointestinal motility of animals.
Drawings
FIG. 1 is a cross-sectional view of a marker of the present invention;
FIG. 2 is a schematic structural view of a marker gripping device of the present invention;
FIG. 3 is a cross-sectional view of a marker gripping device of example 1 of the present invention;
fig. 4 is a cross-sectional view of the marker gripping device of example 2 of the present invention.
Wherein: 1. a marker; 1a, a marker kernel;
1b, a marker inner shell; 1c, a marker shell;
2. a grip portion; third, the connecting part;
4. a controller; fifth, the suction part;
6. a push rod; and seventhly, through holes.
Detailed Description
The invention is further described below with reference to the drawings and examples.
As shown in fig. 1 and 2, an animal gastrointestinal motility detection system comprises a marker 1 and a marker grasping device; the marker 1 is of a layered structure, and comprises a marker inner core 1a, a marker inner shell 1b and a marker outer shell 1c in sequence from inside to outside, wherein the marker inner core 1a is a mixture of iron or barium which is impermeable to X rays; the marker gripping device comprises a gripping part 2, a controller 4 and an elongated connecting part 3 for connecting the gripping part and the controller; the tail end of the gripping part 2 forms an opening for accommodating the marker 1; the controller 4 controls the gripping or releasing of the marker 1 by the gripping portion 2.
The invention can use the markers 1 with different inner diameters and densities according to the inner diameter of esophagus and daily food density of the target animal.
The marker housing 1c is made of silica gel.
The marker 1 is spherical, ellipsoidal or short bar-shaped.
The length of the connecting part 3 is about 5cm, a graduated scale is arranged on the outer wall of the connecting part 3, and the insertion depth can be controlled according to the graduated scale, so that the insertion depth can be accurately positioned at the esophagus entrance.
The magnet is a permanent magnet or an electromagnet.
Example 1
As shown in fig. 3, the inside of the gripping part 2 and the connecting part 3 is of a cavity structure; the tail end of the gripping part 2 is internally provided with an attraction part 5 with a through hole 7 in the center, and the attraction part 5 is a magnet and is attracted with a marker inner shell 1b consisting of iron or a magnet; one end of the pushing rod 6 penetrates into a through hole 7 in the center of the suction part 5, and the other end penetrates through the connecting part 3 to be connected with the controller 4; a stepping motor is arranged in the controller 4, and a rotor of the stepping motor is connected with one end of the pushing rod 6.
The stepping motor is driven forward, the pushing rod 6 moves forward, the pushing rod 6 pushes the marker 1 to be separated from the suction part 5, and then the marker 1 is released to the upper end of the esophagus of the experimental animal; the stepping motor is driven reversely, the pushing rod 6 moves backwards, the other marker 1 and the suction part 5 are mutually attracted, and the cycle of releasing the next marker 1 is entered.
Example 2
As shown in fig. 4, the inside of the gripping part 2 and the connecting part 3 is of a cavity structure; the tail end of the gripping part 2 is internally provided with an attraction part 5 with a through hole 7 in the center, and the attraction part 5 is a magnet and is attracted with a marker inner shell 1b consisting of iron or a magnet; the controller 4 controls the gripping or releasing of the marker 1 by the gripping part 2 through air pressure or hydraulic pressure.
The air pressure is formed by an air pump; the fluid pressure is created by a syringe or hydraulic cylinder and the injected fluid may be a contrast agent such as iohexol.
A certain amount of gas or liquid is injected into the connecting part 3 and the gripping part 2 to generate thrust, so that the marker 1 is pushed to be separated from the suction part 5, and then enters the upper end of the esophagus of the experimental animal; after the first marker 1 is released, the other marker 1 and the suction part 5 are mutually sucked, and the cycle of releasing the next marker 1 is entered.
The gastrointestinal motility detection method of the experimental animal comprises the following steps: after the animals were anesthetized, the outline of the digestive tract was first outlined with a certain amount of iohexol at a certain concentration (in the case of mice, 0.1ml of iohexol at a concentration of 320mg/ml was administered to each mouse). Then releasing the marker 1 from the upper end of the esophagus of the experimental animal through the mouth to enable the marker 1 to be discharged to the anus side along with the movement of the digestive tract of the experimental animal, and obtaining the following parameters in the process of removing the marker 1:
esophageal movement function parameters: the esophagus is put intoMouth (Release marker 1 position is defined as X 1 ) The cardiac orifice is defined as X 2 Maximum diameter of esophagus X 3 ～X 4 . Record X 1 To X 2 The distance L between them (with the X-ray opaque scale as the actual length reference) and the time of movement T of the marker 1 through the two points is detected. After detection, esophageal movement function indexes can be obtained: the maximum diameter of esophagus of the experimental animal is X 3 -X 4 A distance; esophageal evacuation rate v=l/T (cm/s); speed of evacuation of a certain segment of the esophagus: the origin of a certain segment of the esophagus is defined as X m The length of the esophagus is L m =X 2 -X m Marker 1 passes through X m To pass through X 2 The time is recorded as T m The esophageal evacuation speed is L m /T m 。
Gastric emptying parameters: dynamic observation under X-ray after the marker 1 enters the cardia, the observation time is prolonged, and the time for the marker 1 to leave the pylorus, which is the gastric emptying time, is measured, wherein the length of the pylorus from the cardia entering to the pylorus exiting of the marker 1 is recorded as L n The whole discharge time is recorded as T n Gastric emptying rate of L n /T n 。
Gastrointestinal tract evacuation parameters: after a plurality of markers 1 enter the cardia, the anesthesia is released, the experimental animal is normally raised, the excreted excrement of the experimental animal is collected periodically, the X-ray examination is carried out, the number of the markers 1in the excrement is counted, and the gastrointestinal tract emptying rate (n) in the interval Row of rows /n Total (S) ) Percent of the total weight of the composition. (if only gastrointestinal tract evacuation exams are performed, it is not necessary to outline the tract in advance of contrast agent)
The patent adopts a double-layer rearing cage for rearing test animals, wherein the double-layer rearing cage has an upper layer and a lower layer, the upper layer is an animal rearing area, and the lower layer is an excrement collecting area; the bottom surface of the upper layer is in a fence shape, and the interval between every two fence bars is the maximum interval for ensuring that animals do not fall into the bottom layer, so that feces are collected; the lower layer bottom surface is provided with a pumping grid which is equal to the upper layer bottom surface in size, so that the excrement can be conveniently and completely collected.
Experimental statistics results
1. Esophageal evacuation parameters in mice
The marker of the animal gastrointestinal motility detection system is tested by 15 repeated experiments to find the emptying speed in the esophagus of the mouse, the average length is 2.6200 +/-0.1897 cm, the average emptying time is 24.4667 +/-4.7640 s, and the average emptying speed is 0.1108 +/-0.0227 cm/s.
2. Esophageal evacuation parameters of mice
The animal gastrointestinal motility detection system marker has the advantages that the marker emptying speed of different length parts in the esophagus of the mouse is measured through 10 repeated experiments, the average length is 1.7220 +/-0.4868 cm, the average emptying time is 15.5600 +/-5.7030 s, and the average emptying speed is 0.1146 +/-0.0234 cm/s.
3. Gastric emptying parameters of mice (markers from cardiac entry to pylorus time of discharge)
The animal gastrointestinal motility detection system has a marker emptying rate in the stomach (cardia to pylorus) of the mouse, an average length of 1.3330+/-0.2062 cm, an average emptying time of 27.1100 +/-3.7230 s and an average emptying rate of 0.0496+/-0.0075 cm/s, which are determined by 10 repeated experiments.
4. Gastrointestinal tract emptying parameters of mice (after 24 hours of marker input, excrement is collected for X-ray fluoroscopy, and emptying rate is counted)
The average emptying rate of the marker of the animal gastrointestinal motility detection system in the gastrointestinal tract of the mice is 89.33 percent by 10 repeated experiments.
Claims (8)
1. An animal gastrointestinal motility detection system, characterized by: comprises a marker (1) and a marker gripping device; the marker (1) is of a layered structure, and comprises a marker inner core (1 a), a marker inner shell (1 b) and a marker outer shell (1 c) from inside to outside, wherein the marker inner core (1 a) is a mixture of iron or barium which is impermeable to X rays; the marker gripping device comprises a gripping part (2), a controller (4) and an elongated connecting part (3) for connecting the gripping part and the controller; the tail end of the gripping part (2) forms an opening for accommodating the marker (1); the controller (4) controls the gripping part (2) to grip or release the marker (1).
2. An animal gastrointestinal motility detection system as claimed in claim 1 wherein: the inside of the gripping part (2) and the connecting part (3) are of a cavity structure; the tail end of the gripping part (2) is internally provided with an attraction part (5) with a through hole (7) at the center, and the attraction part (5) is a magnet and is attracted with a marker inner shell (1 b) consisting of iron or a magnet; one end of the pushing rod (6) penetrates into a through hole (7) in the center of the suction part (5), and the other end of the pushing rod penetrates through the connecting part (3) to be connected with the controller (4); a stepping motor is arranged in the controller (4), and a rotor of the stepping motor is connected with one end of the pushing rod (6).
3. An animal gastrointestinal motility detection system as claimed in claim 1 wherein: the inside of the gripping part (2) and the connecting part (3) are of a cavity structure; the tail end of the gripping part (2) is internally provided with an attraction part (5) with a through hole (7) at the center, and the attraction part (5) is a magnet and is attracted with a marker inner shell (1 b) consisting of iron or a magnet; the controller (4) controls the gripping or loosening of the marker (1) by the gripping part (2) through air pressure or hydraulic pressure.
4. A gastrointestinal motility detection system for animals according to claim 1, 2 or 3, wherein: the marker shell (1 c) is made of silica gel.
5. A gastrointestinal motility detection system for animals according to claim 1, 2 or 3, wherein: the marker (1) is spherical, ellipsoidal or short bar-shaped.
6. A gastrointestinal motility detection system for animals according to claim 1, 2 or 3, wherein: the outer wall of the connecting part (3) is provided with a graduated scale.
7. A gastrointestinal motility detection system for animals according to claim 2 or 3, wherein: the magnet is a permanent magnet or an electromagnet.
8. A method of detecting an animal gastrointestinal motility detection system according to any one of claims 1 to 3 wherein: the method comprises the following steps:
a. after the animals are anesthetized, a certain amount of iohexol with a certain concentration is poured into the esophagus cavity of the experimental animal to outline the digestive tract;
b. releasing the marker (1) from the upper end of the esophagus of the experimental animal through the mouth, so that the marker is discharged to the anus side along with the movement of the digestive tract of the experimental animal, and acquiring relevant parameters in the process of removing the marker (1);
c. calculating esophageal movement function parameters: positioning the esophageal entrance release marker (1) as X 1 The cardiac orifice is positioned at X 2 The method comprises the steps of carrying out a first treatment on the surface of the Record X 1 To X 2 Distance L between the two points, and detecting the movement time T of the marker (1) passing through the two points, and the esophageal evacuation speed: v=l/T (cm/s); speed of evacuation of a certain segment of the esophagus: the starting point of a certain section of the esophagus is defined as X m The length of the esophagus is L m =X 2 -X m Marker (1) passes through X m To pass through X 2 The time is recorded as T m The esophageal evacuation speed is L m /T m ；
d. Calculating gastric emptying parameters: recording the time from the entrance of the marker (1) to the exit of the pylorus, and the entrance of the marker (1) to the exit of the pylorusThe gate length is defined as L n The whole discharge time is recorded as T n Gastric emptying rate of L n /T n ；
e. Calculating gastrointestinal tract evacuation parameters: releasing a plurality of markers (1) at the upper end of the esophagus of the experimental animal, after the markers (1) enter the cardia, removing anesthesia, raising normally, periodically collecting the excreted feces of the experimental animal, performing X-ray examination, counting the number of the markers (1) in the feces, and performing gastrointestinal tract emptying rate in the time interval: (n) Row of rows /n Total (S) ）%。
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711383093.0A CN108030471B (en) | 2017-12-20 | 2017-12-20 | Animal gastrointestinal motility detection system and detection method |
Applications Claiming Priority (1)
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