CN108014136A - A kind of preparation and application of squid ink black pigment chelating calcium tablet - Google Patents
A kind of preparation and application of squid ink black pigment chelating calcium tablet Download PDFInfo
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- CN108014136A CN108014136A CN201710858914.5A CN201710858914A CN108014136A CN 108014136 A CN108014136 A CN 108014136A CN 201710858914 A CN201710858914 A CN 201710858914A CN 108014136 A CN108014136 A CN 108014136A
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- A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
- A61K35/56—Materials from animals other than mammals
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- A61K9/20—Pills, tablets, discs, rods
- A61K9/2004—Excipients; Inactive ingredients
- A61K9/2022—Organic macromolecular compounds
- A61K9/205—Polysaccharides, e.g. alginate, gums; Cyclodextrin
- A61K9/2059—Starch, including chemically or physically modified derivatives; Amylose; Amylopectin; Dextrin
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Abstract
The invention discloses a kind of preparation of squid ink black pigment chelating calcium tablet, including：Pitch-dark pigment, the preparation of polypeptide chelate iron, the preparation of polypeptide chelate calcium, tablet preparation are obtained, squid ink black pigment chelating calcium tablet can be swallowed or chewed or containing food, and be eaten suitable for multiple-age bracket crowd.Have the beneficial effect that：Effective calcium component content in tablet is high, is easily absorbed by the body, and pitch-dark pigment has antimicrobial antiviral activity, also has the function that stronger anti-oxidant and radicals scavenging, can strengthen the immunocompetence of human body；The mouthfeel of calcium tablet is excellent, stability is good, and disintegration effect is strong, can accelerate drug-eluting；Production technology is feasible, quality controllable, suitable for industrialized production；Tablet can strengthen immunity of organisms by adjusting the stable state of antioxidant system, have certain lead-eliminating effect to blood lead and liver lead, while have protective effect to the copper in liver, the zinc in kidney and the zinc in brain while replenishing the calcium.
Description
Technical field
Preparation and application field the present invention relates to tablet, more particularly, to a kind of squid ink black pigment chelating calcium tablet
Prepare and apply.
Technical background
Element necessary to calcium is biology.For human body, no matter in muscle, nerve, body fluid and bone, all useful Ca2+Knot
The protein of conjunction.Calcium is the main inorganic composition of mankind's bone, tooth, and neurotransmission, contraction of muscle, blood clotting, hormone are released
Put and the necessary element such as galactosis.Calcium participates in metabolism, must be supplemented with calcium daily；Calcium content deficiency or mistake in human body
It is surplus all to influence growth and development and health.Calcium is the inorganic salts component that content is most in human body, and calcium is total in health adult's body
About 1000-1300 grams, about the 1.5%-2.0% of percentage of liveweight of amount, wherein 99% calcium is present in bone and tooth in the form of bone salts
In tooth, remaining is distributed in soft tissue, and the calcium in extracellular fluid only accounts for the 0.1% of total calcium content.Bone is the main portions of doped calcium,
So there is the title in " calcium storehouse ".Bone calcium mainly exists in the form of two kinds of the hydroxyapatite of non-crystal calcium monohydrogen phosphate and crystal, its group
Constantly changed with Human Physiology or pathologic condition into materialization property.Calcium monohydrogen phosphate is more than outmoded bone in area of new bone, bone into
Gradually it is transformed into hydroxyapatite during ripe.Bone makes bone calcium keep dynamic flat with blood calcium by continuous skeletonization and osteolysis
Weighing apparatus.
In the generation of contained large biological molecule polymer, claims in squid prepared Chinese ink, is that known can uniquely protect organisms from
The native endogenous biopolymer of radiation injury.Containing the multiple biological activities such as polypeptide fragments, acidic polysaccharose, tyrosinase into
Point.With removing free radical and combining the characteristic of genotoxic potential ion (such as some transition metal), application potential is huge.Squid
Pitch-dark pigment belongs to natural eumelanin class, containing functional groups such as hydroxyl, carboxyl, amino or amide groups, has preferable metal
Ion chelating activity.
Technology of preparing on calcium tablet has many methods, and the prior art is 103356496 B of CN as authorized public platform
Chinese invention patent, disclose a kind of citric acid calcium malate drug tablet, the inventive method is former by citric acid and calcium malate
Material direct powder compression obtain tablet, have highly dissoluble, high-selenium corn usability, do not influence human body to iron absorb, it is safe and non-toxic,
The characteristics of gastrointestinal acid-base balance will not be destroyed, but its only with citric acid calcium malate as tablet, cause its tablet
Without sweet taste, mouthfeel is poor, and its tablet causes its easy moisture absorption to go bad, influence quality without any coating material.
The content of the invention
The purpose of this method is to provide a kind of preparation and application of squid ink black pigment chelating calcium tablet, pitch-dark with squid
Pigment chelates calcium as tablet material, and tablet stability is strong, the absorptivity of calcium, and also there is tablet anti-oxidant, enhancing to be immunized
The effect of power.
The present invention is directed to the problem of being mentioned in background technology, and the technical solution taken is：A kind of squid ink black pigment chelating
The preparation and application of calcium tablet, including：Obtain pitch-dark pigment, the preparation of polypeptide chelate iron, the preparation of polypeptide chelate calcium, tablet system
Standby, application, specifically includes following steps：
Obtain pitch-dark pigment：Squid fish body belly is splitted, takes out ink sac, cuts off cyst membrane, extrudes prepared Chinese ink, it is isometric pure with 0-4 DEG C
Water soaked overnight, 11000-12000r/min are centrifuged 10-25 minutes, and adjusting pH is 7.3-7.5, and temperature is 48-55 DEG C, adds alkali
Property protease 4200-4500U/g, add the 4- hydroxylphenylethyl alcohols of prepared Chinese ink weight 0.5-0.7%, when hydrolysis 2-4 is small, then
Heat up enzyme deactivation, and 12000-14000r/min is centrifuged 10-15 minutes, washes, is centrifuged to neutrality repeatedly, taking precipitate is in 65-75 DEG C
It is dry, obtain melanin；Enzymatic Extraction squid ink black pigment has preferable hydrolysis effect, and operation is simple, and avoiding makes
It is environmentally friendly with toxic organic solvent, available for industrialized production；The micro 4- hydroxylphenylethyl alcohols added in enzymolysis process have
Help reduce the energy gradient between enzyme digestion reaction ground state and activated state, accelerate extent of reaction, greatly improve enzyme digestion reaction
Efficiency, saves time cost and cost of labor；
The preparation of polypeptide chelate iron：Squid albumen is hydrolyzed, by 4.1-4.5 parts of squid meat, 100-120 parts of distilled water, temperature
40-45 DEG C of degree, neutral proteinase 2000-4000U/g, food flavor enzyme 2000-4000U/g, the condition that pH value is 6-7 are hydrolyzed,
Inactivated after when hydrolysis 1.5-2 is small, filter to obtain hydrolyzate；The ferric chloride solution of 1-1.5 parts of 0.1-0.12mol/L is taken to be added to
In 100-150 parts of hydrolyzates, 0.1-0.12 parts of ascorbic acid is added, adjusting pH is 7.0-7.2, and temperature is 20-23 DEG C, magnetic force
Stirring chelating is centrifuged except precipitation after 15-30 minutes, is freeze-dried after concentration, it is spare to be placed in 0-4 DEG C of preservation in drying basin；Hydrolyze squid
Fish protein prepares polypeptide, and it is stand-by then to prepare polypeptide chelate chalybeate；
The preparation of polypeptide chelate calcium：Pipette 20-30 parts of 9-12mmol/L calcium chloride solutions to be placed in glass reaction bottle, add squid
Pitch-dark pigment 0.018-0.023 parts, 0.003-0.005 parts of polypeptide chelate chalybeate, D-4- hydroxyphenylglycines 0.001-0.0015
Part, adjustings pH value of solution be 3.8-4.5, inflated with nitrogen avoid light place, shaking table 50-55r/min vibrate adsorb 8-10 it is small when, after taking-up
Centrifugation, takes precipitation to be drying to obtain pitch-dark pigment chelating calcium；This method stability is strong, adsorption rate is high, and does not have during chelating
The secondary organic reagent of other poison is added, is adapted to industrialized production；
It is prepared by tablet：Weigh respectively 40-45 parts of calcium of pitch-dark pigment chelating, 40-42 parts of microcrystalline cellulose, 10-15 parts of filler,
20-25 parts of calcium monohydrogen phosphate, 10-15 parts of hydroxypropyl methyl cellulose, 1.5-2.0 parts of superfine silica gel powder, 0.2-0.3 parts of vitamin D in
It is uniformly mixed in wet granulator, adds 10-15 parts of softwoods processed of 50-55% ethanol, gained softwood is used on oscillating granulator
20-24 mesh nylon screen is pelletized, and gained particle is placed in fluid bed to dry, whole grain, is added 2-3 parts of magnesium stearates and is always mixed, will
Total mixed gained particle is made the circle stamping of diameter 6-7mm scrobiculas with rotary pelleting machine and is coated to obtain the final product；Effective calcium in tablet
Component content is high, is easily absorbed by the body, and pitch-dark pigment has antimicrobial antiviral activity, also with stronger anti-oxidant and
The effect of radicals scavenging, can strengthen the immunocompetence of human body；The mouthfeel of calcium tablet is excellent, stability is good, and disintegration effect is strong,
It can accelerate drug-eluting；Production technology is feasible, quality controllable, suitable for industrialized production；
Using：Chelating calcium tablet can be swallowed or chewed or containing food, and be eaten suitable for multiple-age bracket crowd；Tablet is being replenished the calcium
Meanwhile, it is capable to strengthen immunity of organisms by the stable state for adjusting antioxidant system, there is certain lead-eliminating effect to blood lead and liver lead,
There is protective effect to the copper in liver, the zinc in kidney and the zinc in brain at the same time.
Preferably, filler is lactose or mannitol or amylum pregelatinisatum；With mobility, compressibility, self-lubrication
Property and adhesiveness, and have preferable calving disaggregation, and be easier to be absorbed by the body.
Preferably, coating material is 20-25 parts of sucrose, 0.045-0.05 parts of neotame, 0.5-0.6 parts of menthol, sorb
20-25 parts of alcohol, 35-40 parts of dextrin, the mixture of 1-1.2 parts of magnesium stearate；Tablet coating stability is preferable, is adapted to scale metaplasia
Production.
Compared with prior art, the advantage of the invention is that：1) effective calcium component content in tablet is high, easily by human body
Absorb, and pitch-dark pigment has antimicrobial antiviral activity, also has the function that stronger anti-oxidant and radicals scavenging, can
Strengthen the immunocompetence of human body；The mouthfeel of calcium tablet is excellent, stability is good, and disintegration effect is strong, can accelerate drug-eluting；Production
Feasible process, it is quality controllable, suitable for industrialized production；2) tablet, can be by adjusting anti-oxidant body while replenishing the calcium
The stable state enhancing immunity of organisms of system, has certain lead-eliminating effect to blood lead and liver lead, while in the copper in liver, kidney
Zinc in zinc and brain has protective effect.
Brief description of the drawings
Fig. 1 is that squid ink black pigment is sweet to the chelation percent and polypeptide chelate chalybeate/D-4- hydroxy benzenes of calcium ion in the present invention
The relation curve schematic diagram of the weight ratio of propylhomoserin.
Embodiment
The present invention program is described further below by embodiment：
Embodiment 1：
A kind of preparation and application of squid ink black pigment chelating calcium tablet, comprise the following steps：
1) take squid and obtain prepared Chinese ink, with 4 DEG C of isometric pure water soaked overnights, 11000r/min is centrifuged 10 minutes, adds alkalescence
Protease hydrolytic, then heat up enzyme deactivation, and 12000r/min is centrifuged 10 minutes, washes, is centrifuged to neutrality repeatedly, taking precipitate is in 65
DEG C drying, obtains melanin；2) inactivated after adding neutral proteinase, flavor enzyme hydrolysis in squid meat, filter to obtain hydrolyzate；Take
The ferric chloride solution of 1ml 0.1mol/L is added in 100ml hydrolyzates, adds the ascorbic acid of 0.1g, and it is 7 to adjust pH, temperature
Spend for 20 DEG C, centrifugation, which removes, after magnetic agitation chelates 15 minutes precipitates, and is freeze-dried after concentration, it is standby to be placed in 0 DEG C of preservation in drying basin
With；3) pipette 9mmol/L calcium chloride solutions 20ml to be placed in glass reaction bottle, add 0.018 part of squid ink black pigment, polypeptide
Chalybeate 0.003g is chelated, it is 3.8 to adjust pH value of solution, inflated with nitrogen avoid light place, when shaking table 50r/min vibrations absorption 8 is small, after taking-up
Centrifugation, takes precipitation to be drying to obtain pitch-dark pigment chelating calcium；This method stability is strong, adsorption rate is high, and does not have during chelating
The secondary organic reagent of other poison is added, is adapted to industrialized production；
4) pitch-dark 40 parts of calcium of pigment chelating, 40 parts of microcrystalline cellulose, 10 parts of mannitol, 20 parts of calcium monohydrogen phosphate, hydroxypropyl are weighed respectively
10 parts of ylmethyl cellulose, 1.5 parts of superfine silica gel powder, 0.2 part of vitamin D are uniformly mixed in wet granulator, add 50% second
10 parts of softwoods processed of alcohol, gained softwood is pelletized on oscillating granulator with 20 mesh nylon screens, gained particle is placed in fluid bed
Middle drying, whole grain, adds 2 parts of magnesium stearates and always mixes, and will always mix gained particle rotary pelleting machine diameter 6mm scrobicula circle stampings
It is coated to obtain the final product；Effective calcium component content in tablet is high, is easily absorbed by the body, and pitch-dark pigment is lived with anti-bacteria and anti-virus
Property, also there is stronger anti-oxidant and radicals scavenging, the immunocompetence of human body can be strengthened.
Embodiment 2：
A kind of preparation and application of squid ink black pigment chelating calcium tablet, including：Obtain pitch-dark pigment, the system of polypeptide chelate iron
Prepared by standby, chelating, tablet, application, specifically includes following steps：
1) pitch-dark pigment is obtained：Squid fish body belly is splitted, takes out ink sac, cuts off cyst membrane, extrudes prepared Chinese ink, it is isometric pure with 4 DEG C
Water soaked overnight, 12000r/min are centrifuged 25 minutes, and it is 7.5 to adjust pH, and temperature is 55 DEG C, adds alkali protease 4500U/g,
The 4- hydroxylphenylethyl alcohols of prepared Chinese ink weight 0.5% are added, when hydrolysis 2 is small, then heat up enzyme deactivation, and 14000r/min centrifuges 15 points
Clock, is washed, centrifugation to neutrality, taking precipitate obtains melanin in 75 DEG C of dryings repeatedly；Enzymatic Extraction squid ink black pigment has
There is preferable hydrolysis effect, operation is simple, avoids using toxic organic solvent, environmentally friendly, available for industrializing
Production；
2) preparation of polypeptide chelate iron：By 4.5 parts of squid meat, 120 parts of distilled water, temperature 45 C, neutral proteinase 4000U/g,
Inactivated after when food flavor enzyme 4000U/g, the condition that pH value is 7 hydrolysis 2 are small, filter to obtain hydrolyzate；Take the chlorine of 1.5 parts of 0.12mol/L
Change ferrous solution to be added in 150 parts of hydrolyzates, add 0.12 part of ascorbic acid, it is 7.2 to adjust pH, and temperature is 23 DEG C, magnetic force
Stirring chelating is centrifuged except precipitation after 30 minutes, is freeze-dried after concentration, it is spare to be placed in 4 DEG C of preservations in drying basin；Hydrolyze squid egg
White matter prepares polypeptide, and it is stand-by then to prepare polypeptide chelate chalybeate；
3) preparation of polypeptide chelate calcium：Pipette 30 parts of 12mmol/L calcium chloride solutions to be placed in glass reaction bottle, add squid ink
0.023 part of melanin, 0.005 part of polypeptide chelate chalybeate, it is 4.5 to adjust pH value of solution, inflated with nitrogen avoid light place, shaking table 55r/min
When vibration absorption 10 is small, centrifuged after taking-up, take precipitation to be drying to obtain pitch-dark pigment chelating calcium；This method stability is strong, adsorption rate
Height, and the secondary organic reagent of other poison is not added during chelating, it is adapted to industrialized production；
4) prepared by tablet：Pitch-dark pigment chelating Ca45 part, 42 parts of microcrystalline cellulose, 15 parts of filler, calcium monohydrogen phosphate are weighed respectively
25 parts, 15 parts of hydroxypropyl methyl cellulose, 2 parts of superfine silica gel powder, 0.3 part of vitamin D in wet granulator be uniformly mixed, add
Enter 55% ethanol, 15 parts of softwoods processed, gained softwood is pelletized on oscillating granulator with 24 mesh nylon screens, gained particle is put
Dry in fluid bed, whole grain, adds 3 parts of magnesium stearates and always mixes, and will always mix gained particle rotary pelleting machine diameter 7mm scrobiculas
To obtain the final product, its coating material is 20 parts of sucrose to circle punching press coating tablets, 0.045 part of neotame, 0.5 part of menthol, 20 parts of sorbierite, dextrin
35 parts, the mixture of 1 part of magnesium stearate；Effective calcium component content in tablet is high, is easily absorbed by the body, and ink black
Element has antimicrobial antiviral activity, also has the function that stronger anti-oxidant and radicals scavenging, can strengthen the immune of human body
Ability.
Embodiment 3：
A kind of preparation and application of squid ink black pigment chelating calcium tablet, including：Obtain pitch-dark pigment, the system of polypeptide chelate iron
Prepared by standby, polypeptide chelate calcium preparation, tablet, application, specifically includes following steps：
Obtain pitch-dark pigment：Squid fish body belly is splitted, takes out ink sac, cuts off cyst membrane, prepared Chinese ink is extruded, with 2 DEG C of isometric pure water
Soaked overnight, 12000r/min are centrifuged 15 minutes, and it is 7.4 to adjust pH, and temperature is 52 DEG C, add alkali protease 4200U/g, then
The 4- hydroxylphenylethyl alcohols of prepared Chinese ink weight 0.7% are added, when hydrolysis 3 is small, then heat up enzyme deactivation, and 14000r/min is centrifuged 15 minutes,
Wash repeatedly, centrifugation to neutrality, taking precipitate obtains melanin in 70 DEG C of dryings；Enzymatic Extraction squid ink black pigment have compared with
Good hydrolysis effect, operation is simple, avoids using toxic organic solvent, environmentally friendly, available for industrialized production；
The preparation of polypeptide chelate iron：By 4.2 parts of squid meat, 100 parts of distilled water, 40 DEG C of temperature, neutral proteinase 3000U/g, wind
Inactivated after when taste enzyme 3000U/g, the condition that pH value is 7 hydrolysis 2 are small, filter to obtain hydrolyzate；Take the iron chloride of 1 part of 0.12mol/L
Solution is added in 120 parts of hydrolyzates, adds 0.1 part of ascorbic acid, and it is 7 to adjust pH, and temperature is 20 DEG C, magnetic agitation chelating
Centrifugation is freeze-dried after concentration except precipitation, it is spare to be placed in 3 DEG C of preservations in drying basin after 15 minutes；Squid protein is hydrolyzed to prepare
Polypeptide, it is stand-by then to prepare polypeptide chelate chalybeate；
The preparation of polypeptide chelate calcium：Pipette 25 parts of 10mmol/L calcium chloride solutions to be placed in glass reaction bottle, it is pitch-dark to add squid
0.020 part of pigment, 0.004 part of polypeptide chelate chalybeate, 0.001 part of D-4- hydroxyphenylglycines, it is 4.0 to adjust pH value of solution, nitrogen charging
Gas avoid light place, when shaking table 55r/min vibrations absorption 8 is small, centrifuges after taking-up, takes precipitation to be drying to obtain pitch-dark pigment chelating calcium；
This method stability is strong, adsorption rate is high, and the secondary organic reagent of other poison is not added during chelating, and is adapted to industry metaplasia
Production；
It is prepared by tablet：Pitch-dark pigment chelating Ca45 part, 40 parts of microcrystalline cellulose, 15 parts of filler, calcium monohydrogen phosphate 20 are weighed respectively
Part, 15 parts of hydroxypropyl methyl cellulose, 2 parts of superfine silica gel powder, 0.3 part of vitamin D are uniformly mixed in wet granulator, are added
50% 12 parts of ethanol softwood processed, gained softwood is pelletized on oscillating granulator with 24 mesh nylon screens, gained particle is placed in
Dry in fluid bed, whole grain, adds 2 parts of magnesium stearates and always mixes, and will always mix gained particle rotary pelleting machine diameter 7mm scrobicula circles
To obtain the final product, coating material is 22 parts of sucrose to punching press coating tablets, 0.045 part of neotame, 0.5 part of menthol, 20 parts of sorbierite, dextrin 35
Part, the mixture of 1.2 parts of magnesium stearate；Effective calcium component content in tablet is high, is easily absorbed by the body, and pitch-dark pigment
With antimicrobial antiviral activity, also there is stronger anti-oxidant and radicals scavenging, the immunity energy of human body can be strengthened
Power；The mouthfeel of calcium tablet is excellent, stability is good, and disintegration effect is strong, can accelerate drug-eluting；Production technology is feasible, and quality can
Control, suitable for industrialized production.
Embodiment 4：
On the basis of embodiment 3, do not change any data of embodiment 3, only by the polypeptide chelate chalybeate in its chelation step/
The parts by weight of D-4- hydroxyphenylglycines are set to 0.03/0.03,0.04/0.02,0.045/0.015,0.04/0.01,0.05/
0.01st, 0.03/0.005,0.042/0.006,0.04/0.005,0.045/0.005,0.05/0.005, i.e. both weight ratios
For 1:1、2:1、……、9:1、10:1.
Using the calcium ion content in solution after chelation step in metric measurement embodiment 1-4, so as to draw squid
The chelated calcium chelation percent of the pitch-dark pigment of fish, by the chelation percent in embodiment 1-3 arrange shown in table 1：
The chelated calcium chelation percent of squid ink black pigment in 1. embodiment 1-3 of table
| Embodiment 1 | Embodiment 2 | Embodiment 3 | |
| Chelation percent | 60.5% | 58.6% | 68.5% |
As shown in Table 1, the suitable chelating condition of control, squid ink black pigment is very high to the chelation percent of calcium ion, can reach
55%-70%, higher chelation percent improve the utilization ratio of squid ink, add the amount of the calcium ion of its absorption, improve
Squid ink black pigment chelates the calcium content of calcium tablet, especially, when adding D-4- hydroxyls in the chelatropic reaction in embodiment 3
After phenylglycine, the chelation percent of squid ink black pigment is improved relative to the embodiment 1-2 for being not added with D-4- hydroxyphenylglycines
Nearly 10%, illustrate to improve squid ink black based on certain unknown inherent mechanism, the addition of D-4- hydroxyphenylglycines
Suction-operated of the element to calcium ion.
The experiment of polypeptide chelate chalybeate in embodiment 4 and D-4- hydroxyphenylglycine different weight part ratios is measured respectively
The relation curve of its chelation percent, chelation percent and the two weight ratio arranges as shown in Figure 1.
As seen from Figure 1, chelation percent and polypeptide chelate chalybeate and D-4- hydroxy benzenes of the squid ink black pigment to calcium ion
There is association in the weight ratio of glycine, and non-linear dependencies are presented in the association；As seen from Figure 1, when polypeptide chelate iron
The weight ratio of agent and D-4- hydroxyphenylglycines is between 3:1-10:When in 1 section, chelating of the squid ink black pigment to calcium ion
Rate is above 60%, especially, when the weight ratio of polypeptide chelate chalybeate and D-4- hydroxyphenylglycines is 3:1 or between 7:1-
8:When in 1 section, squid ink black pigment has reached more than 80% to the chelation percent of calcium ion, this is probably due to D-4- hydroxyls
Phenylglycine acts on polypeptide chelate chalybeate and has discharged environment in certain unknown substance change chelating, so as to improve squid
The pitch-dark pigment of fish is to the adsorption capacity of calcium ion, so as to improve its chelation percent, its internal mechanism is still not clear, squid ink black
Element helps to improve content calcareous in tablet to the higher chelation percent of calcium ion, while enhances the utilization of squid ink black pigment
Value.
Routine operation in the operating procedure of the present invention is well known to those skilled in the art, herein without repeating.
Technical scheme is described in detail in embodiment described above, it should be understood that the above is only
For the specific embodiment of the present invention, it is not intended to limit the invention, all any modifications made in the spirit of the present invention,
Supplement or similar fashion replacement etc., should all be included in the protection scope of the present invention.
Claims (8)
1. a kind of preparation of squid ink black pigment chelating calcium tablet, including：Obtain the preparation, more of pitch-dark pigment, polypeptide chelate iron
It is prepared by the chelated calcium preparation of peptide, tablet, it is characterised in that：The chelation step is：Pipetting calcium chloride solution, to be placed in glass anti-
Answer in bottle, add squid ink black pigment, polypeptide chelate chalybeate, D-4- hydroxyphenylglycines, adjusting pH value of solution is 3.8-4.5, is filled
Nitrogen avoid light place, when shaking table 50-55r/min vibration absorption 8-10 is small, centrifuges after taking-up, takes precipitation to be drying to obtain pitch-dark pigment
Chelate calcium.
A kind of 2. preparation of squid ink black pigment chelating calcium tablet according to claim 1, it is characterised in that：Described obtains
The pitch-dark pigment step is taken to be：Squid fish body belly is splitted, takes out ink sac, cuts off cyst membrane, extrudes prepared Chinese ink, it is isometric pure with 0-4 DEG C
Water soaked overnight, 11000-12000r/min are centrifuged 10-25 minutes, and adjusting pH is 7.3-7.5, and temperature is 48-55 DEG C, adds alkali
Property protease 4200-4500U/g, add the 4- hydroxylphenylethyl alcohols of prepared Chinese ink weight 0.5-0.7%, when hydrolysis 2-4 is small, Ran Housheng
Warm enzyme deactivation, 12000-14000r/min are centrifuged 10-15 minutes, are washed repeatedly, are centrifuged to neutrality, taking precipitate and done in 65-75 DEG C
It is dry, obtain melanin.
A kind of 3. preparation of squid ink black pigment chelating calcium tablet according to claim 1, it is characterised in that：Described is more
The preparation process of peptide chelated iron is：Squid albumen is hydrolyzed, by 4.1-4.5 parts of squid meat, 100-120 parts of distilled water, temperature
40-45 DEG C of degree, neutral proteinase 2000-4000 U/g, food flavor enzyme 2000-4000U/g, the condition that pH value is 6-7 hydrolyze, hydrolysis
Inactivated after when 1.5-2 is small, filter to obtain hydrolyzate；The ferric chloride solution of 1-1.5 parts of 0.1-0.12mol/L is taken to be added to 100-150
In part hydrolyzate, 0.1-0.12 parts of ascorbic acid is added, adjusting pH is 7-7.2, and temperature is 20-23 DEG C, magnetic agitation chelating
Centrifugation is freeze-dried after concentration except precipitation, it is spare to be placed in 0-4 DEG C of preservation in drying basin after 15-30 minutes.
A kind of 4. preparation of squid ink black pigment chelating calcium tablet according to claim 1, it is characterised in that：Described is more
The chelated calcium preparation process of peptide is：Pipette 20-30 parts of 9-12mmol/L calcium chloride solutions to be placed in glass reaction bottle, add squid
Pitch-dark pigment 0.018-0.023 parts, 0.003-0.005 parts of polypeptide chelate chalybeate, D-4- hydroxyphenylglycines 0.001-0.0015
Part, adjustings pH value of solution be 3.8-4.5, inflated with nitrogen avoid light place, shaking table 50-55r/min vibrate adsorb 8-10 it is small when, after taking-up from
The heart, takes precipitation to be drying to obtain pitch-dark pigment chelating calcium.
A kind of 5. preparation of squid ink black pigment chelating calcium tablet according to claim 1, it is characterised in that：The piece
Agent preparation process is：Pitch-dark 40-45 parts of calcium of pigment chelating, 40-42 parts of microcrystalline cellulose, 10-15 parts of filler, phosphorus are weighed respectively
Sour 20-25 parts of hydrogen calcium, 10-15 parts of hydroxypropyl methyl cellulose, 1.5-2 parts of superfine silica gel powder, 0.2-0.3 parts of vitamin D are in wet method
It is uniformly mixed in granulator, adds 10-15 parts of softwoods processed of 50-55% ethanol, gained softwood is used into 20-24 on oscillating granulator
Mesh nylon screen is pelletized, and gained particle is placed in fluid bed to dry, whole grain, is added 2-3 parts of magnesium stearates and is always mixed, will always mix institute
Particle is obtained the circle stamping of diameter 6-7mm scrobiculas is made with rotary pelleting machine and is coated to obtain the final product.
A kind of 6. preparation of squid ink black pigment chelating calcium tablet according to claim 5, it is characterised in that：The piece
Filler in agent preparation process is：Lactose or mannitol or amylum pregelatinisatum.
A kind of 7. preparation of squid ink black pigment chelating calcium tablet according to claim 5, it is characterised in that：The piece
Coating material in agent preparation process is：Coating material is 20-25 parts of sucrose, 0.045-0.05 parts of neotame, menthol 0.5-0.6
Part, 20-25 parts of sorbierite, 35-40 parts of dextrin, the mixture of 1-1.2 parts of magnesium stearate.
A kind of 8. application of squid ink black pigment chelating calcium tablet, it is characterised in that：The squid ink black pigment chelating calcium tablet
Agent can be swallowed or chewed or containing food, and be eaten suitable for multiple-age bracket crowd.
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| CN108014136B (en) | 2020-07-24 |
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