CN108008141A - A kind of microwell plate sample adding device and microwell plate sample adding system - Google Patents
A kind of microwell plate sample adding device and microwell plate sample adding system Download PDFInfo
- Publication number
- CN108008141A CN108008141A CN201711228125.XA CN201711228125A CN108008141A CN 108008141 A CN108008141 A CN 108008141A CN 201711228125 A CN201711228125 A CN 201711228125A CN 108008141 A CN108008141 A CN 108008141A
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- CN
- China
- Prior art keywords
- microwell plate
- sample adding
- adding device
- indicator board
- microwell
- Prior art date
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- 239000000463 material Substances 0.000 claims description 3
- 238000013461 design Methods 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 229920003023 plastic Polymers 0.000 description 3
- 239000004033 plastic Substances 0.000 description 3
- 238000004146 energy storage Methods 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 125000006850 spacer group Chemical group 0.000 description 2
- 230000002159 abnormal effect Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000008014 freezing Effects 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
- 230000001788 irregular Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
- G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
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- Physics & Mathematics (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The invention discloses a kind of microwell plate sample adding device and microwell plate sample adding system, specific microwell plate sample adding device disclosed by the invention includes card slot and indicator board；The card slot is arranged for accommodating microwell plate；The indicator board includes plate body and scale portion, and the scale portion is arranged for being identified the micropore on the microwell plate；The side wall of the card slot offers the notch of the plate body push-and-pull move for the indicator board, and a part for the plate body is stretched in the card slot from the notch.Using the microwell plate sample adding device of the present invention, microwell plate is put into card slot by when sample-adding, and indicator board is pushed forward to the correspondence row for making scale portion be located at microwell plate, required for can easily indicating plus row and corresponding row number, reduction loading errors；The microwell plate sample adding device of the present invention is additionally provided with storage chamber, it can be ensured that low temperature environment is in during sample-adding.
Description
Technical field
The present invention relates to experimental instrument field, more particularly to a kind of microwell plate sample adding device and microwell plate sample adding system.
Background technology
PCR generally uses 96 orifice plates or 384 orifice plates with microwell plate, when carrying out sample-adding operation, since hole is more, very close
Collection, the spacing of Kong Yukong is very small, between Kong Yukong without obvious separation mark, so when being loaded with pipettor, often
Obscure or omit there is a situation where row or column is loaded, cause experimental result abnormal.Therefore, need to operate during sample-adding
Personnel's spirit high concentration, significantly increases the stress of staff.
One piece of 96 orifice plate or 384 orifice plates are further encountered in testing at the same time to be needed to add different reaction solutions in different zones
Scene, in the case of this irregular sample-adding, this causes automatic sample arm system to be difficult to operate, manually in the case of sample-adding more
Easily make a fault.
The content of the invention
The purpose of the present invention is to solve during existing sample-adding easily error, work efficiency is low the problem of.In addition,
Low temperature environment is generally required during microwell plate sample-adding, it is therefore desirable to a kind of microwell plate sample-adding dress for being capable of providing low temperature environment
Put.
To achieve the above object, the first aspect of the present invention provides a kind of microwell plate sample adding device, and the microwell plate adds
Sampling device includes card slot and indicator board；The card slot is arranged for accommodating microwell plate；The indicator board includes plate body and scale
Portion, the scale portion are arranged for being identified the micropore on the microwell plate；The side wall of the card slot is offered for institute
The notch of the plate body push-and-pull move of indicator board is stated, a part for the plate body is stretched in the card slot from the notch.
Further, the card slot includes a storage chamber, when the microwell plate is located in the card slot, the microwell plate
Above the storage chamber.During use, ice cube can be placed in the storage chamber, for providing low temperature environment.
Further, the ice storage chamber outer wall is made of heat-barrier material.
Further, press strip and elastomeric element (such as spring) are included in the notch, the elastomeric element is located at the groove
Between the top surface and the press strip of mouth, the perpendicular wall of the notch is equipped with sliding slot, and the both ends of the press strip are equipped with protrusion, described
In in the sliding slot, the press strip can be moved up and down along the sliding slot for protruding parts；The indicator board is located at the press strip and institute
Between the bottom surface for stating notch.
Further, the indicator board upper surface is equipped with equidistant groove, and the bottom surface of the press strip is equipped with and the groove
Matched protrusion.
Further, the outer ledge of the indicator board is additionally provided with a baffle, and the baffle is vertical with the indicator board, institute
The thickness for stating baffle is more than the length of the height of the notch, and/or the length of the baffle more than the notch.
Further, the scale portion includes first flag area and second identifier area, and use is set in the first flag area
In the micropore for identifying 96 orifice plates；The second identifier area is arranged for the micropore of 384 orifice plates of mark.
Further, the scale cross section is in trapezoid.
Further, the scale cross section is in trapezoid, and front end forms an inclined-plane；The foldable connection in scale portion
In the plate body of the indicator board；The bottom surface in the scale portion is equipped with the first flag area, and the inclined-plane in the scale portion is set
There is the second identifier area.The scale portion has folded state and extended configuration.
It is preferably carried out in another kind in mode, the scale portion includes a containing cavities and a mark rod, the mark rod
Cross section is in trapezoid, and front end forms an inclined-plane；The mark rod can be received into the containing cavities；The containing cavities top
Surface is equipped with the first flag area, and the inclined-plane of the mark rod is equipped with the second identifier area.
The second aspect of the present invention provides a kind of microwell plate sample adding system, and the microwell plate sample adding system includes the present invention
The microwell plate sample adding device that provides of first aspect, and microwell plate group；The microwell plate group includes one or more microwell plates.
In another preference, the microwell plate group includes one or more 96 hole microwell plates.
In another preference, the microwell plate group includes one or more 384 hole microwell plates.
In another preference, the microwell plate group includes at least one 96 hole microwell plate and at least one 384 hole micropore
Plate.
Technique effect
Using the microwell plate sample adding device of the present invention, microwell plate is put into card slot by when sample-adding, and indicator board, which is pushed forward, makes mark
Ruler portion is located at the correspondence row of microwell plate, and the row added required for can easily indicating and corresponding row number, reduce loading errors；This
The microwell plate sample adding device of invention is additionally provided with storage chamber, it can be ensured that low temperature environment is in during sample-adding.
It is described further below with reference to the technique effect of design of the attached drawing to the present invention, concrete structure and generation, with
It is fully understood from the purpose of the present invention, feature and effect.
Brief description of the drawings
Fig. 1 is the planar structure schematic diagram of the microwell plate sample adding device of the preferred embodiment of the present invention.
Fig. 2 is the cross-sectional view of the microwell plate sample adding device of the preferred embodiment of the present invention.
Fig. 3 is the planar structure signal of the microwell plate sample adding device of the use state of the preferred embodiment of the present invention
Figure.
Fig. 4 is scale portion partial enlargement structural representation, show rulers portion folded state.
Fig. 5 is scale portion partial enlargement structural representation, show rulers portion extended configuration.
Fig. 6 is scale portion partial enlargement structural representation in another preferred embodiment.
Fig. 7 is the cross-sectional view of microwell plate sample adding device in another preferred embodiment.
Embodiment
Embodiment 1
As shown in Figure 1, Figure 2, Figure 3 shows, a kind of microwell plate sample adding device, the microwell plate sample adding device are present embodiments provided
Including card slot 1 and indicator board 2；The card slot 1 is arranged for accommodating microwell plate 01；The indicator board 2 includes plate body 22 and mark
Ruler portion 21, the scale portion 21 are arranged for being identified the micropore on the microwell plate 01；The side wall of the card slot 1 is opened
The notch 11 moved equipped with 22 pull of plate body for the indicator board 2, a part for the plate body 22 are stretched into from the notch 11
In to the card slot 1.
The card slot 1 includes a storage chamber 12, when the microwell plate 01 is located in the card slot 1, the microwell plate 01
Above the storage chamber 12.During use, ice cube can be placed in the storage chamber 12, for providing low temperature environment.
Water can also be put into storage chamber 12 and then carried out freezing and obtain ice cube before the use.12 outer wall of storage chamber by every
Hot material is made.Any spacer can be not provided between storage chamber 12 and microwell plate 01, i.e. microwell plate 01 is placed directly within ice face
On；The spacer of heat conduction can also be set.
Storage chamber 12 can be separated independently of card slot 1, i.e. storage chamber 12 from card slot 1；Storage chamber 12 can also be made
For a part for 1 groove body of card slot.
As shown in Fig. 2, including press strip 13 and elastomeric element 14 in the notch 11, the elastomeric element 14 is located at the groove
Between the top surface and the press strip 13 of mouth 11, the perpendicular wall of the notch 11 is equipped with sliding slot, and sliding slot is vertically arranged, the press strip 13
Both ends be equipped with protrusion 131, the protrusion 131 is located in the sliding slot, and the press strip 13 can be moved down along the sliding slot
It is dynamic；The indicator board 2 is between the press strip 13 and the bottom surface of the notch 11.Under the action of elastomeric element 14, press strip
13 can provide the power for being pressed downward the indicator board 2 so that the indicator board 2 is close on microwell plate 01.Notch 11 has one
Fixed height, can allow indicator board 2 to move up and down.For example in some cases, the indoor ice face of ice storage is excessive, causes microwell plate
01 position is excessive, if it is indicated that plate 2 can not move up and down, can cause to show that plate 2 can not cover the top of microwell plate 01.And this reality
Apply in example, indicator board 2 can move up and down, therefore can be applicable in the microwell plate 01 of different height.
As shown in Figure 1 and Figure 2, the position of indicator board 2 is adjusted for convenience, and the present embodiment is in 2 plate body 22 of indicator board
Upper surface is provided with equidistant groove 221, and the bottom surface of the press strip 13 is equipped with and the groove 221 matched raised 132.It is recessed
Groove 221 is hemispherical groove, and correspondingly described raised 132 be hemispherical projections.The distance of adjacent grooves 221 can be equal to 384
The distance between adjacent cells on the microwell plate of hole.When pushing and pulling indicator board 2, easily indicator board 2 can be positioned.
The outer ledge of the indicator board 2 is additionally provided with a baffle 23, and the baffle 23 is vertical with the indicator board 2, described
The thickness of baffle 23 is more than the height of the notch 11, and/or the length of the baffle 23 is more than the length of the notch 11.Gear
Plate 23 can be used as force application part, since baffle 23 is higher than and/or is longer than the notch 11, indicator board 2 will not completely into
Enter in card slot 1.
The scale portion 21 includes first flag area 211 and second identifier area 212, and the first flag area 211 is set
For identifying the micropore of 96 hole microwell plates；The second identifier area 212 is arranged for the micropore of 384 hole microwell plates of mark.The
One tag slot 211 is identified with the row number on 96 hole microwell plates；Second identifier area 212 is identified with the row number on 384 hole microwell plates.
As shown in Figure 4, Figure 5,21 cross section of scale portion is in trapezoid, and front end forms an inclined-plane；The scale portion 21
The foldable plate body 22 for being connected to the indicator board 2；The bottom surface in the scale portion 21 is equipped with the first flag area 211, described
The inclined-plane in scale portion 21 is equipped with the second identifier area 212.The scale portion 21 has folded state (Fig. 4) and stretch-like
State (Fig. 5).
The problem of being run into during being loaded for PCR, devises a set of microwell plate sample adding device, can easily and fast, it is accurate
Really sample-adding.PCR sample-addings generally require low temperature environment at the same time, and the microwell plate sample adding device of the present embodiment is equipped with storage chamber, it is ensured that adds
Solution is in low temperature environment during sample.
Operation principle is as follows：Be loaded housing design into drawing and pulling type structure, small drawer design into frozen type energy storage ice chest, it is necessary to
Before sample-adding, ice chest -20 is frozen, frozen type energy storage ice chest is inserted into porous sample-adding recorder during sample-adding, can maintain 1h's or so
Low temperature environment, meets requirement for low temperature during PCR sample-addings.
At the same time sample-adding housing on PCR plate line direction set a slidable plastics fillet, plastics fillet both sides according to
384 or 96 orifice plates row number corresponding numeral is set respectively, such as 96 orifice plates, set from 1-12,384 orifice plates, set 1-24.
Plus when shift plastics fillet onto corresponding row, required for can easily indicating plus row and corresponding row number, reduce sample-adding and miss
Difference.
Embodiment 2
As shown in fig. 6, microwell plate sample adding device basic structure provided in this embodiment, with embodiment 1, difference is, described
Scale portion 21 includes a containing cavities 213 and a mark rod 214, and 214 cross section of mark rod is in trapezoid, and it is oblique that front end forms one
Face.The mark rod 214 can be received into the containing cavities 213；The surface on 213 top of containing cavities is equipped with the described first mark
Know area 211, the inclined-plane of the mark rod is equipped with the second identifier area 212.The mark can be adjusted by a bolt 215
The position of bar 214.
Embodiment 3
As shown in fig. 7, microwell plate sample adding device basic structure provided in this embodiment, with embodiment 1, difference is, is storing up
Icehouse 12 can be embedded in the card slot 1 independently of card slot 1,1 bottom opening of card slot, storage chamber 12.1 inner wall of card slot is equipped with as institute
State the support arm that microwell plate 01 provides support.
In other embodiments, 1 sidewall opening of card slot, storage chamber 12 pass in and out card slot 1 in a manner of push-and-pull.
Independently storage chamber 12 can more easily ice making, it is cold without whole microwell plate sample adding device is put into refrigerator
Freeze.
Preferred embodiment of the invention described in detail above.It should be appreciated that those of ordinary skill in the art without
Need creative work to conceive according to the present invention and make many modifications and variations.Therefore, all technologies in the art
Personnel are available by logical analysis, reasoning, or a limited experiment on the basis of existing technology under this invention's idea
Technical solution, all should be in the protection domain being defined in the patent claims.
Claims (10)
1. a kind of microwell plate sample adding device, it is characterised in that the microwell plate sample adding device includes card slot and indicator board；It is described
Card slot is arranged for accommodating microwell plate；The indicator board includes plate body and scale portion, and the scale portion is arranged for institute
The micropore stated on microwell plate is identified；The side wall of the card slot offers the groove of the plate body push-and-pull move for the indicator board
Mouthful, a part for the plate body is stretched in the card slot from the notch.
2. microwell plate sample adding device as claimed in claim 1, it is characterised in that the card slot includes a storage chamber, when described
When microwell plate is located in the card slot, the microwell plate is located above the storage chamber.
3. microwell plate sample adding device as claimed in claim 2, it is characterised in that the ice storage chamber outer wall is by heat-barrier material system
Into.
4. microwell plate sample adding device as claimed in claim 1, it is characterised in that include press strip and elastic portion in the notch
Part, the elastomeric element are located between top surface and the press strip of the notch, and the perpendicular wall of the notch is equipped with sliding slot, described
The both ends of press strip are equipped with protrusion, and in the sliding slot, the press strip can be moved up and down along the sliding slot for the protruding parts；Institute
Indicator board is stated between the press strip and the bottom surface of the notch.
5. microwell plate sample adding device as claimed in claim 4, it is characterised in that the indicator board upper surface is equipped with equidistantly recessed
Groove, the bottom surface of the press strip are equipped with the protrusion with the fit depressions.
6. microwell plate sample adding device as claimed in claim 1, it is characterised in that the outer ledge of the indicator board is additionally provided with one
Baffle, the baffle is vertical with the indicator board, and the thickness of the baffle is more than the height of the notch, and/or the baffle
Length be more than the notch length.
7. microwell plate sample adding device as claimed in claim 1, it is characterised in that the scale portion includes first flag area and the
Two tag slots, the first flag area are arranged for the micropore of 96 hole microwell plates of mark；Use is set in the second identifier area
In the micropore for identifying 384 hole microwell plates；Preferably, the scale cross section is in trapezoid.
8. microwell plate sample adding device as claimed in claim 1, it is characterised in that the scale cross section is in trapezoid, preceding
End forms an inclined-plane；The foldable plate body for being connected to the indicator board in scale portion；The bottom surface in the scale portion is equipped with described
First flag area, the inclined-plane in the scale portion are equipped with the second identifier area；Or
The scale portion includes a containing cavities and a mark rod, and the mark rod cross section is in trapezoid, and front end forms an inclined-plane；
The mark rod can be received into the containing cavities；The surface on the containing cavities top is equipped with the first flag area, the mark
The inclined-plane for knowing bar is equipped with the second identifier area.
A kind of 9. microwell plate sample adding system, it is characterised in that the microwell plate described in the microwell plate sample adding system bag claim 1
Sample adding device, and microwell plate group；The microwell plate group includes one or more microwell plates.
10. microwell plate sample adding system as claimed in claim 9, it is characterised in that the microwell plate group includes at least one 96
Hole microwell plate and at least one 384 hole microwell plate.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711228125.XA CN108008141B (en) | 2017-11-29 | 2017-11-29 | Microplate sample adding device and microplate sample adding system |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711228125.XA CN108008141B (en) | 2017-11-29 | 2017-11-29 | Microplate sample adding device and microplate sample adding system |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN108008141A true CN108008141A (en) | 2018-05-08 |
| CN108008141B CN108008141B (en) | 2024-04-12 |
Family
ID=62054649
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711228125.XA Active CN108008141B (en) | 2017-11-29 | 2017-11-29 | Microplate sample adding device and microplate sample adding system |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN108008141B (en) |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110317714A (en) * | 2019-08-14 | 2019-10-11 | 北京市中医研究所 | The PCR microwell plate adapter of low temperature tape light |
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2017
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| CN110317714A (en) * | 2019-08-14 | 2019-10-11 | 北京市中医研究所 | The PCR microwell plate adapter of low temperature tape light |
Also Published As
| Publication number | Publication date |
|---|---|
| CN108008141B (en) | 2024-04-12 |
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