CN107969684A - The method of lactic acid bacteria and saccharomycete symbiotic fermentation Sparassis crispa - Google Patents
The method of lactic acid bacteria and saccharomycete symbiotic fermentation Sparassis crispa Download PDFInfo
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- CN107969684A CN107969684A CN201711157052.XA CN201711157052A CN107969684A CN 107969684 A CN107969684 A CN 107969684A CN 201711157052 A CN201711157052 A CN 201711157052A CN 107969684 A CN107969684 A CN 107969684A
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- China
- Prior art keywords
- sparassis crispa
- saccharomycete
- lactic acid
- acid bacteria
- stoste
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- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 title claims abstract description 118
- 241000272503 Sparassis radicata Species 0.000 title claims abstract description 95
- 241000894006 Bacteria Species 0.000 title claims abstract description 73
- 239000004310 lactic acid Substances 0.000 title claims abstract description 59
- 235000014655 lactic acid Nutrition 0.000 title claims abstract description 59
- 241000235342 Saccharomycetes Species 0.000 title claims abstract description 53
- 238000000855 fermentation Methods 0.000 title claims abstract description 42
- 230000004151 fermentation Effects 0.000 title claims abstract description 42
- 238000000034 method Methods 0.000 title claims abstract description 14
- 241000209140 Triticum Species 0.000 claims abstract description 35
- 235000021307 Triticum Nutrition 0.000 claims abstract description 35
- 210000001161 mammalian embryo Anatomy 0.000 claims abstract description 35
- 239000002994 raw material Substances 0.000 claims abstract description 35
- 239000000203 mixture Substances 0.000 claims abstract description 17
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims description 27
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 22
- 239000000706 filtrate Substances 0.000 claims description 17
- 239000000047 product Substances 0.000 claims description 14
- 240000004808 Saccharomyces cerevisiae Species 0.000 claims description 12
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 claims description 12
- 239000003814 drug Substances 0.000 claims description 11
- 238000003756 stirring Methods 0.000 claims description 11
- 239000000843 powder Substances 0.000 claims description 7
- BJEPYKJPYRNKOW-REOHCLBHSA-N (S)-malic acid Chemical compound OC(=O)[C@@H](O)CC(O)=O BJEPYKJPYRNKOW-REOHCLBHSA-N 0.000 claims description 4
- TVXBFESIOXBWNM-UHFFFAOYSA-N Xylitol Natural products OCCC(O)C(O)C(O)CCO TVXBFESIOXBWNM-UHFFFAOYSA-N 0.000 claims description 4
- BJEPYKJPYRNKOW-UHFFFAOYSA-N alpha-hydroxysuccinic acid Natural products OC(=O)C(O)CC(O)=O BJEPYKJPYRNKOW-UHFFFAOYSA-N 0.000 claims description 4
- 239000001630 malic acid Substances 0.000 claims description 4
- 235000011090 malic acid Nutrition 0.000 claims description 4
- HEBKCHPVOIAQTA-UHFFFAOYSA-N meso ribitol Natural products OCC(O)C(O)C(O)CO HEBKCHPVOIAQTA-UHFFFAOYSA-N 0.000 claims description 4
- 230000001954 sterilising effect Effects 0.000 claims description 4
- 235000010447 xylitol Nutrition 0.000 claims description 4
- 239000000811 xylitol Substances 0.000 claims description 4
- HEBKCHPVOIAQTA-SCDXWVJYSA-N xylitol Chemical compound OC[C@H](O)[C@@H](O)[C@H](O)CO HEBKCHPVOIAQTA-SCDXWVJYSA-N 0.000 claims description 4
- 229960002675 xylitol Drugs 0.000 claims description 4
- 241000186018 Bifidobacterium adolescentis Species 0.000 claims description 3
- 241000186016 Bifidobacterium bifidum Species 0.000 claims description 3
- 240000001046 Lactobacillus acidophilus Species 0.000 claims description 3
- 235000013956 Lactobacillus acidophilus Nutrition 0.000 claims description 3
- 244000199866 Lactobacillus casei Species 0.000 claims description 3
- 235000013958 Lactobacillus casei Nutrition 0.000 claims description 3
- 241000186605 Lactobacillus paracasei Species 0.000 claims description 3
- 240000006024 Lactobacillus plantarum Species 0.000 claims description 3
- 235000013965 Lactobacillus plantarum Nutrition 0.000 claims description 3
- 241000194020 Streptococcus thermophilus Species 0.000 claims description 3
- 229940002008 bifidobacterium bifidum Drugs 0.000 claims description 3
- 235000015165 citric acid Nutrition 0.000 claims description 3
- 229940039695 lactobacillus acidophilus Drugs 0.000 claims description 3
- 229940017800 lactobacillus casei Drugs 0.000 claims description 3
- 229940072205 lactobacillus plantarum Drugs 0.000 claims description 3
- 241001478240 Coccus Species 0.000 claims description 2
- 241000218588 Lactobacillus rhamnosus Species 0.000 claims description 2
- 238000004659 sterilization and disinfection Methods 0.000 claims description 2
- 210000000481 breast Anatomy 0.000 claims 3
- 239000007788 liquid Substances 0.000 abstract description 10
- 230000000694 effects Effects 0.000 abstract description 8
- FYGDTMLNYKFZSV-URKRLVJHSA-N (2s,3r,4s,5s,6r)-2-[(2r,4r,5r,6s)-4,5-dihydroxy-2-(hydroxymethyl)-6-[(2r,4r,5r,6s)-4,5,6-trihydroxy-2-(hydroxymethyl)oxan-3-yl]oxyoxan-3-yl]oxy-6-(hydroxymethyl)oxane-3,4,5-triol Chemical compound O[C@@H]1[C@@H](O)[C@H](O)[C@@H](CO)O[C@H]1OC1[C@@H](CO)O[C@@H](OC2[C@H](O[C@H](O)[C@H](O)[C@H]2O)CO)[C@H](O)[C@H]1O FYGDTMLNYKFZSV-URKRLVJHSA-N 0.000 abstract description 7
- 229920002498 Beta-glucan Polymers 0.000 abstract description 7
- 230000036541 health Effects 0.000 abstract description 7
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 abstract description 6
- 239000008280 blood Substances 0.000 abstract description 4
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- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 abstract description 4
- 230000011132 hemopoiesis Effects 0.000 abstract description 4
- 230000002218 hypoglycaemic effect Effects 0.000 abstract description 4
- 150000002632 lipids Chemical class 0.000 abstract description 4
- 150000001413 amino acids Chemical class 0.000 abstract description 3
- 230000003712 anti-aging effect Effects 0.000 abstract description 3
- 230000001857 anti-mycotic effect Effects 0.000 abstract description 3
- 239000002543 antimycotic Substances 0.000 abstract description 3
- 238000006243 chemical reaction Methods 0.000 abstract description 3
- 235000012000 cholesterol Nutrition 0.000 abstract description 3
- 238000011161 development Methods 0.000 abstract description 3
- 230000018109 developmental process Effects 0.000 abstract description 3
- 230000036039 immunity Effects 0.000 abstract description 3
- 238000012545 processing Methods 0.000 abstract description 3
- 230000000840 anti-viral effect Effects 0.000 abstract description 2
- 230000001737 promoting effect Effects 0.000 abstract 1
- 238000000605 extraction Methods 0.000 description 8
- 239000000284 extract Substances 0.000 description 6
- 150000004676 glycans Chemical class 0.000 description 4
- 239000000463 material Substances 0.000 description 4
- 229920001282 polysaccharide Polymers 0.000 description 4
- 239000005017 polysaccharide Substances 0.000 description 4
- 235000001674 Agaricus brunnescens Nutrition 0.000 description 3
- 230000031068 symbiosis, encompassing mutualism through parasitism Effects 0.000 description 3
- QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 description 2
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 2
- 229920001503 Glucan Polymers 0.000 description 2
- 238000012356 Product development Methods 0.000 description 2
- 239000003963 antioxidant agent Substances 0.000 description 2
- 230000003078 antioxidant effect Effects 0.000 description 2
- 235000006708 antioxidants Nutrition 0.000 description 2
- 238000004090 dissolution Methods 0.000 description 2
- 235000013376 functional food Nutrition 0.000 description 2
- 238000004806 packaging method and process Methods 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 230000000717 retained effect Effects 0.000 description 2
- 230000000638 stimulation Effects 0.000 description 2
- OILXMJHPFNGGTO-UHFFFAOYSA-N (22E)-(24xi)-24-methylcholesta-5,22-dien-3beta-ol Natural products C1C=C2CC(O)CCC2(C)C2C1C1CCC(C(C)C=CC(C)C(C)C)C1(C)CC2 OILXMJHPFNGGTO-UHFFFAOYSA-N 0.000 description 1
- RQOCXCFLRBRBCS-UHFFFAOYSA-N (22E)-cholesta-5,7,22-trien-3beta-ol Natural products C1C(O)CCC2(C)C(CCC3(C(C(C)C=CCC(C)C)CCC33)C)C3=CC=C21 RQOCXCFLRBRBCS-UHFFFAOYSA-N 0.000 description 1
- OQMZNAMGEHIHNN-UHFFFAOYSA-N 7-Dehydrostigmasterol Natural products C1C(O)CCC2(C)C(CCC3(C(C(C)C=CC(CC)C(C)C)CCC33)C)C3=CC=C21 OQMZNAMGEHIHNN-UHFFFAOYSA-N 0.000 description 1
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 description 1
- SHZGCJCMOBCMKK-UHFFFAOYSA-N D-mannomethylose Natural products CC1OC(O)C(O)C(O)C1O SHZGCJCMOBCMKK-UHFFFAOYSA-N 0.000 description 1
- DNVPQKQSNYMLRS-NXVQYWJNSA-N Ergosterol Natural products CC(C)[C@@H](C)C=C[C@H](C)[C@H]1CC[C@H]2C3=CC=C4C[C@@H](O)CC[C@]4(C)[C@@H]3CC[C@]12C DNVPQKQSNYMLRS-NXVQYWJNSA-N 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 208000002682 Hyperkalemia Diseases 0.000 description 1
- 206010020772 Hypertension Diseases 0.000 description 1
- 206010021036 Hyponatraemia Diseases 0.000 description 1
- DGAQECJNVWCQMB-PUAWFVPOSA-M Ilexoside XXIX Chemical compound C[C@@H]1CC[C@@]2(CC[C@@]3(C(=CC[C@H]4[C@]3(CC[C@@H]5[C@@]4(CC[C@@H](C5(C)C)OS(=O)(=O)[O-])C)C)[C@@H]2[C@]1(C)O)C)C(=O)O[C@H]6[C@@H]([C@H]([C@@H]([C@H](O6)CO)O)O)O.[Na+] DGAQECJNVWCQMB-PUAWFVPOSA-M 0.000 description 1
- SHZGCJCMOBCMKK-JFNONXLTSA-N L-rhamnopyranose Chemical compound C[C@@H]1OC(O)[C@H](O)[C@H](O)[C@H]1O SHZGCJCMOBCMKK-JFNONXLTSA-N 0.000 description 1
- PNNNRSAQSRJVSB-UHFFFAOYSA-N L-rhamnose Natural products CC(O)C(O)C(O)C(O)C=O PNNNRSAQSRJVSB-UHFFFAOYSA-N 0.000 description 1
- 241000186660 Lactobacillus Species 0.000 description 1
- 208000001132 Osteoporosis Diseases 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- 208000004880 Polyuria Diseases 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- 229930182558 Sterol Natural products 0.000 description 1
- 229930003268 Vitamin C Natural products 0.000 description 1
- 229930003316 Vitamin D Natural products 0.000 description 1
- QYSXJUFSXHHAJI-XFEUOLMDSA-N Vitamin D3 Natural products C1(/[C@@H]2CC[C@@H]([C@]2(CCC1)C)[C@H](C)CCCC(C)C)=C/C=C1\C[C@@H](O)CCC1=C QYSXJUFSXHHAJI-XFEUOLMDSA-N 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000004913 activation Effects 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- 229910021529 ammonia Inorganic materials 0.000 description 1
- 230000003110 anti-inflammatory effect Effects 0.000 description 1
- 230000003064 anti-oxidating effect Effects 0.000 description 1
- 230000000259 anti-tumor effect Effects 0.000 description 1
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 230000033228 biological regulation Effects 0.000 description 1
- 210000000988 bone and bone Anatomy 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 150000001720 carbohydrates Chemical class 0.000 description 1
- 235000014633 carbohydrates Nutrition 0.000 description 1
- 235000013325 dietary fiber Nutrition 0.000 description 1
- 230000035619 diuresis Effects 0.000 description 1
- DNVPQKQSNYMLRS-SOWFXMKYSA-N ergosterol Chemical compound C1[C@@H](O)CC[C@]2(C)[C@H](CC[C@]3([C@H]([C@H](C)/C=C/[C@@H](C)C(C)C)CC[C@H]33)C)C3=CC=C21 DNVPQKQSNYMLRS-SOWFXMKYSA-N 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 235000001497 healthy food Nutrition 0.000 description 1
- 230000001900 immune effect Effects 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 229940039696 lactobacillus Drugs 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 208000005368 osteomalacia Diseases 0.000 description 1
- 229910052760 oxygen Inorganic materials 0.000 description 1
- 239000001301 oxygen Substances 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 150000003254 radicals Chemical class 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 208000007442 rickets Diseases 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 229910052708 sodium Inorganic materials 0.000 description 1
- 150000003432 sterols Chemical class 0.000 description 1
- 235000003702 sterols Nutrition 0.000 description 1
- 230000003612 virological effect Effects 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 235000019154 vitamin C Nutrition 0.000 description 1
- 239000011718 vitamin C Substances 0.000 description 1
- 235000019166 vitamin D Nutrition 0.000 description 1
- 239000011710 vitamin D Substances 0.000 description 1
- 150000003710 vitamin D derivatives Chemical class 0.000 description 1
- 229940046008 vitamin d Drugs 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L31/00—Edible extracts or preparations of fungi; Preparation or treatment thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
- A23L29/065—Microorganisms
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/113—Acidophilus
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/125—Casei
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/165—Paracasei
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/169—Plantarum
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/175—Rhamnosus
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/21—Streptococcus, lactococcus
- A23V2400/249—Thermophilus
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/51—Bifidobacterium
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/51—Bifidobacterium
- A23V2400/513—Adolescentes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/51—Bifidobacterium
- A23V2400/519—Breve
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/51—Bifidobacterium
- A23V2400/533—Longum
Abstract
A kind of lactic acid bacteria of the present invention and the method for saccharomycete symbiotic fermentation Sparassis crispa, it is formulated the raw material for including following parts by weight：Sparassis crispa extracting solution, wheat embryo extracting solution, flavouring, lactic acid bacteria and saccharomycete.The lactic acid bacteria of the present invention and the composition of raw materials of saccharomycete symbiotic fermentation Sparassis crispa stoste, using Sparassis crispa as primary raw material, add wheat embryo, biofermentation conversion is carried out using the synbiosis of lactic acid bacteria and saccharomycete therebetween, fermented type Sparassis crispa stoste is made, it is easier by human consumption, absorbed, help immune and resist the disease ability the effect for improving human body.Fermenation raw liquid prepared by the present invention contains a large amount of amino acid and beta glucan, it can strengthen and improve the immunity of the human body, is antiviral, is antimycotic, is hypoglycemic, stimulate hematopoiesis, reduce cholesterol, reducing blood lipid, the good effect of anti-ageing health care of waiting for a long time, having great importance to the plantation for promoting Sparassis crispa with intensive processing and its industrialized development.
Description
Technical field
The invention belongs to technical field of biological fermentation, and in particular to a kind of lactic acid bacteria and saccharomycete symbiotic fermentation Sparassis crispa
Method.
Background technology
Sparassis crispa, also known as silk ball mushroom, Classification system are Sparassis crispa, are non-skirt pore fungi mesh, silk ball Cordycepps, embroider
Coccus.Sparassis crispa is rare food medicine dual-purpose bacterium, is known as " kings of ten thousand mushrooms ", because of its activation immunocompetence with superelevation,
There is the title of " illusion mystery mushroom " in Japan.Sparassis crispa contains substantial amounts of protein, carbohydrate, multivitamin, a variety of ammonia
Base acid and dietary fiber and ash content, mineral matter etc..Wherein, Sparassis crispa contains substantial amounts of glucan, and glucan is that a kind of biology is living
Property material, have immunological regulation, antitumor, anti-inflammatory, antiviral, anti-oxidant, radioresistance, hypoglycemic, reducing blood lipid, liver protection, raising
The multiple functions such as hematopoiesis function；Vitamin Ε, vitamin C in Sparassis crispa have antioxidation；Sparassis crispa also contains ergot
Sterol, ergosterol can be changed into vitamin D under sunlight and ultraviolet irradiation, promote the absorption of calcium, phosphorus, be conducive to bone
Formed, children rachitis, adult's osteoporosis and osteomalacia can be prevented；Potassium content is very high in Sparassis crispa, and sodium contains
Measure relatively low, hyperkalemia hyponatremia plays the role of diuresis, highly beneficial to hypertension；Contain substantial amounts of polyphenoils in Sparassis crispa
Matter, such as hepatocuprein, can effectively remove internal free radical, prevent infringement of the active oxygen to body, have anti-oxidant, anti-
The effect of aging.
Due to the special efficacy of Sparassis crispa, the research to it at present is just tending to be popular.And it is directed to its product development
Also like a raging fire, emerge in the existing many associated products of Japan.Its patent is at present up to 52, and Japan accounts among these
The overwhelming majority.Secondly South Korea, and China is very little for the patent of Sparassis crispa product development at present.Utilize silk ball now
The effect of bacterium comes across market with particle, powdery more as healthy food, these added values of product are few, and Sparassis crispa is unique and nutrition
Do not utilize well.
The content of the invention
An object of the present invention is to provide a kind of lactic acid bacteria and the raw material of saccharomycete symbiotic fermentation Sparassis crispa stoste to match somebody with somebody
Side.
A kind of lactic acid bacteria of the present invention and the composition of raw materials of saccharomycete symbiotic fermentation Sparassis crispa stoste, including following parts by weight
Several raw materials：60 parts~80 parts of Sparassis crispa extracting solution, 20 parts~40 parts of wheat embryo extracting solution, 0.1 part~0.5 part of flavouring,
0.01 part~0.03 part of 0.01 part~0.03 part of lactic acid bacteria and saccharomycete.
The lactic acid bacteria of the present invention and the composition of raw materials of saccharomycete symbiotic fermentation Sparassis crispa stoste, using Sparassis crispa to be main former
Material, adds wheat embryo, carries out biofermentation conversion using the synbiosis of lactic acid bacteria and saccharomycete therebetween, fermentation is made
Type Sparassis crispa stoste, makes it be easier by human consumption, absorb, and helps immune and resist the disease ability the effect for improving human body
Power.Fermenation raw liquid prepared by the present invention contains a large amount of amino acid and beta glucan, can strengthen and improve the immunity of the human body, is disease-resistant
Malicious, antimycotic, hypoglycemic, stimulation hematopoiesis, reduce cholesterol, reducing blood lipid, the good effect of anti-ageing health care of waiting for a long time, not only can be direct
Sparassis crispa health care oral liquid is made in packaging；Also can be added to using Sparassis crispa stoste as a kind of raw material with healthcare function other
In health products and other functional foods, the plantation to promotion Sparassis crispa has important with intensive processing and its industrialized development
Meaning.
In addition, above-mentioned lactic acid bacteria of the invention and the composition of raw materials of saccharomycete symbiotic fermentation Sparassis crispa stoste, can also have
There is technical characteristic additional as follows：
As the preferred embodiment of the present invention, the lactic acid bacteria and the raw material of saccharomycete symbiotic fermentation Sparassis crispa stoste
Formula, includes the raw material of following parts by weight：60 parts of Sparassis crispa extracting solution, 40 parts of wheat embryo extracting solution, 0.1 part of flavouring,
0.01 part of 0.03 part of lactic acid bacteria and saccharomycete.
As the preferred embodiment of the present invention, the lactic acid bacteria and the raw material of saccharomycete symbiotic fermentation Sparassis crispa stoste
Formula, includes the raw material of following parts by weight：80 parts of Sparassis crispa extracting solution, 20 parts of wheat embryo extracting solution, 0.5 part of flavouring,
0.03 part of 0.01 part of lactic acid bacteria and saccharomycete.
As the preferred embodiment of the present invention, the lactic acid bacteria and the raw material of saccharomycete symbiotic fermentation Sparassis crispa stoste
Formula, includes the raw material of following parts by weight：70 parts of Sparassis crispa extracting solution, 30 parts of wheat embryo extracting solution, 0.25 part of flavouring,
0.02 part of 0.02 part of lactic acid bacteria and saccharomycete.
Further, the lactic acid bacteria includes at least lactobacillus paracasei, streptococcus thermophilus, lactobacillus plantarum, rhamnose
In lactobacillus, newborn Bifidobacterium Bifidum, lactobacillus acidophilus, Lactobacillus casei, bifidobacterium adolescentis, the long double dry bacterium of qi and the short dry bacterium of double qis
Two kinds.
Further, the saccharomycete includes at least one kind in brewer's yeast and Saccharomyces cerevisiae.
Further, the flavouring is at least one kind in citric acid, malic acid and xylitol.
It is another object of the present invention to propose the lactic acid bacteria and the side of saccharomycete symbiotic fermentation Sparassis crispa stoste
Method.
The lactic acid bacteria and the method for saccharomycete symbiotic fermentation Sparassis crispa stoste, include the following steps：S101：By silk ball
Bacterium grind into powder, is subsequently placed in Chinese medicine extracting tank, is 1 according still further to the weight ratio of Sparassis crispa and water：(10~20) water is added,
Then 2.5h~3.5h is extracted at a temperature of 80 DEG C~100 DEG C, is then centrifuged for separating and filters, regather filtrate, obtain silk ball
Bacterium extracting solution；S102：Wheat embryo is placed in Chinese medicine extracting tank, is 1 according still further to the weight ratio of wheat embryo and water：(5~8)
Add water and stir, 2.5h~3.5h is then extracted at a temperature of 60 DEG C~75 DEG C, is then centrifuged for separating and filters, regather filter
Liquid, obtains wheat embryo extracting solution；S103：The Sparassis crispa extracting solution and the wheat embryo extracting solution are mixed and be concentrated into
Relative density is 1.02~1.04, is subsequently placed in fermentation tank, adds the flavouring and stir evenly, then described in addition
Citric acid adjusts its pH value as 5.5~6.5；S104：Stood after the saccharomycete is mixed with the product of the step S103
3min~8min, is stirred for, and then stands 30min~2h；S105：The lactic acid bacteria is added into the product of the step S104
And stir, then ferment 48h~72h at a temperature of 25 DEG C~35 DEG C, then filters and collects filtrate, then the filtrate is gone out
Bacterium, obtains lactic acid bacteria and the symbiotically fermented Sparassis crispa stoste of saccharomycete.
The present invention contains a large amount of polysaccharide using the Sparassis crispa stoste of hot-water process's extraction, compared to alkalinity extraction, beta glucan
Labile risk in alkaline conditions, can be effectively retained the beta glucan of higher degree, compared to acidity extraction, reduce other
The dissolution of impurity, improves the purity of polysaccharide.
Further, it is the mesh of 180 mesh~220 by the size of the Sparassis crispa powder in the step S101.
Further, in the step S105, sterilising temp be 110 DEG C~120 DEG C, sterilization time for 20min~
30min。
The additional aspect and advantage of the present invention will be set forth in part in the description, and will partly become from the following description
Obtain substantially, or recognized by the practice of the present invention.
Embodiment
The embodiment of the present invention is described below in detail, the embodiment is exemplary, it is intended to for explaining the present invention, and
It is not considered as limiting the invention.
Embodiment 1
Embodiment 1 proposes the composition of raw materials of a kind of lactic acid bacteria and saccharomycete symbiotic fermentation Sparassis crispa stoste, including as follows
The raw material of parts by weight：60 parts of Sparassis crispa extracting solution, 40 parts of wheat embryo extracting solution, 0.1 part of citric acid, 0.03 part of lactic acid bacteria and
0.01 part of brewer's yeast.
Wherein, lactic acid bacteria includes lactobacillus paracasei, streptococcus thermophilus and each 0.01 part of lactobacillus plantarum.
The method that embodiment 1 also proposed the lactic acid bacteria and saccharomycete symbiotic fermentation Sparassis crispa stoste, including it is as follows
Step：
(1) it is 180 mesh powders Sparassis crispa to be ground into size, is subsequently placed in Chinese medicine extracting tank, according still further to Sparassis crispa
Weight ratio with water is 1：20 add water, then extract 3.5h at a temperature of 80 DEG C, are then centrifuged for separating and filter, regather filter
Liquid, obtains Sparassis crispa extracting solution.
(2) wheat embryo is placed in Chinese medicine extracting tank, is 1 according still further to the weight ratio of wheat embryo and water：5 add water simultaneously
Stirring, then extracts 2.5h at a temperature of 75 DEG C, is then centrifuged for separating and filters, regather filtrate, obtains wheat embryo extraction
Liquid.
(3) it is 1.04 to mix and be concentrated into relative density the Sparassis crispa extracting solution and the wheat embryo extracting solution,
It is subsequently placed in fermentation tank, adds the citric acid and stir evenly, then adds the citric acid and adjust its pH value and be
5.5。
(4) 8min is stood after the brewer's yeast is mixed with the product of the step (3), is stirred for, then stands
30min。
(5) lactic acid bacteria is added into the product of the step (4) and is stirred, then ferment 48h at a temperature of 35 DEG C,
Then filter and collect filtrate, then the filtrate is sterilized 20min at a temperature of 120 DEG C, obtain lactic acid bacteria and saccharomycete symbiosis
The Sparassis crispa stoste of fermentation.
Embodiment 2
Embodiment 2 proposes the composition of raw materials of a kind of lactic acid bacteria and saccharomycete symbiotic fermentation Sparassis crispa stoste, including as follows
The raw material of parts by weight：80 parts of Sparassis crispa extracting solution, 20 parts of wheat embryo extracting solution, 0.5 part of xylitol, 0.01 part of lactic acid bacteria and
0.03 part of Saccharomyces cerevisiae.
Wherein, lactic acid bacteria includes 0.002 part of Lactobacillus rhamnosus, 0.005 part of newborn Bifidobacterium Bifidum and lactobacillus acidophilus 0.003
Part.
The method that embodiment 2 also proposed the lactic acid bacteria and saccharomycete symbiotic fermentation Sparassis crispa stoste, including it is as follows
Step：
(1) it is 220 mesh powders Sparassis crispa to be ground into size, is subsequently placed in Chinese medicine extracting tank, according still further to Sparassis crispa
Weight ratio with water is 1：10 add water, then extract 2.5h at a temperature of 100 DEG C, are then centrifuged for separating and filter, regather
Filtrate, obtains Sparassis crispa extracting solution.
(2) wheat embryo is placed in Chinese medicine extracting tank, is 1 according still further to the weight ratio of wheat embryo and water：8 add water simultaneously
Stirring, then extracts 3.5h at a temperature of 60 DEG C, is then centrifuged for separating and filters, regather filtrate, obtains wheat embryo extraction
Liquid.
(3) it is 1.02 to mix and be concentrated into relative density the Sparassis crispa extracting solution and the wheat embryo extracting solution,
It is subsequently placed in fermentation tank, adds the xylitol and stir evenly, then adds the citric acid and adjust its pH value and be
6.5。
(4) 3min is stood after the Saccharomyces cerevisiae is mixed with the product of the step (3), is stirred for, then stands 2h.
(5) lactic acid bacteria is added into the product of the step (4) and is stirred, then ferment 72h at a temperature of 25 DEG C,
Then filter and collect filtrate, then the filtrate is sterilized 30min at a temperature of 110 DEG C, obtain lactic acid bacteria and saccharomycete symbiosis
The Sparassis crispa stoste of fermentation.
Embodiment 3
Embodiment 3 proposes the composition of raw materials of a kind of lactic acid bacteria and saccharomycete symbiotic fermentation Sparassis crispa stoste, including as follows
The raw material of parts by weight：70 parts of Sparassis crispa extracting solution, 30 parts of wheat embryo extracting solution, 0.25 part of malic acid, 0.02 part of lactic acid bacteria,
0.01 part of 0.01 part of Saccharomyces cerevisiae and brewer's yeast.
Wherein, lactic acid bacteria includes 0.005 part of Lactobacillus casei, 0.008 part of bifidobacterium adolescentis, the dry bacterium 0.003 of long double qis
Part and short double dry 0.004 part of bacterium of qi.
The method that embodiment 3 also proposed the lactic acid bacteria and saccharomycete symbiotic fermentation Sparassis crispa stoste, including it is as follows
Step：
(1) it is 200 mesh powders Sparassis crispa to be ground into size, is subsequently placed in Chinese medicine extracting tank, according still further to Sparassis crispa
Weight ratio with water is 1：15 add water, then extract 3h at a temperature of 90 DEG C, are then centrifuged for separating and filter, regather filter
Liquid, obtains Sparassis crispa extracting solution.
(2) wheat embryo is placed in Chinese medicine extracting tank, is 1 according still further to the weight ratio of wheat embryo and water：7 add water simultaneously
Stirring, then extracts 3h at a temperature of 68 DEG C, is then centrifuged for separating and filters, regather filtrate, obtain wheat embryo extracting solution.
(3) it is 1.03 to mix and be concentrated into relative density the Sparassis crispa extracting solution and the wheat embryo extracting solution,
It is subsequently placed in fermentation tank, adds the malic acid and stir evenly, then adds the citric acid and adjust its pH value and be
6.0。
(4) 5min is stood after mixing the Saccharomyces cerevisiae and the brewer's yeast with the product of the step (3), then is stirred
Mix, then stand 1.2h.
(5) lactic acid bacteria is added into the product of the step (4) and is stirred, then ferment 60h at a temperature of 30 DEG C,
Then filter and collect filtrate, then the filtrate is sterilized 25min at a temperature of 115 DEG C, obtain lactic acid bacteria and saccharomycete symbiosis
The Sparassis crispa stoste of fermentation.
The lactic acid bacteria as made from embodiment 1-3 and saccharomycete symbiotic fermentation Sparassis crispa stoste, measure through experiment, different
Under preparation condition, the content of the beta glucan of acquisition is followed successively by 378mg/g, 386mg/g and 383mg/g.
To sum up, lactic acid bacteria of the invention and the composition of raw materials of saccharomycete symbiotic fermentation Sparassis crispa stoste, based on Sparassis crispa
Raw material is wanted, adds wheat embryo, biofermentation conversion is carried out using the synbiosis of lactic acid bacteria and saccharomycete therebetween, is made
Fermented type Sparassis crispa stoste, makes it be easier by human consumption, absorb, helps and improve the immune and resist the disease ability of human body
Effect.Fermenation raw liquid prepared by the present invention contains a large amount of amino acid and beta glucan, can strengthen and improve the immunity of the human body, resist
Viral, antimycotic, hypoglycemic, stimulation hematopoiesis, reduce cholesterol, reducing blood lipid, the good effect of anti-ageing health care of waiting for a long time, not only can be straight
Connect packaging and Sparassis crispa health care oral liquid is made；Also it can be added to using Sparassis crispa stoste as a kind of raw material with healthcare function
In its health products and other functional foods, the plantation to promotion Sparassis crispa has important with intensive processing and its industrialized development
Meaning.The present invention contains a large amount of polysaccharide using the Sparassis crispa stoste of hot-water process's extraction, compared to alkalinity extraction, avoids β-Portugal from gathering
Sugar risk labile in alkaline conditions, can be effectively retained the beta glucan of higher degree, compared to acidity extraction, reduce it
The dissolution of his miscellaneous affair, improves the purity of polysaccharide.
In the description of this specification, reference term " one embodiment ", " some embodiments ", " example ", " specifically show
The description of example " or " some examples " etc. means specific features, structure, material or the spy for combining the embodiment or example description
Point is contained at least one embodiment of the present invention or example.In the present specification, schematic expression of the above terms is not
It must be directed to identical embodiment or example.Moreover, particular features, structures, materials, or characteristics described can be in office
Combined in an appropriate manner in one or more embodiments or example.In addition, without conflicting with each other, the skill of this area
Art personnel can be tied the different embodiments or example described in this specification and different embodiments or exemplary feature
Close and combine.
Although the embodiment of the present invention has been shown and described above, it is to be understood that above-described embodiment is example
Property, it is impossible to limitation of the present invention is interpreted as, those of ordinary skill in the art within the scope of the invention can be to above-mentioned
Embodiment is changed, changes, replacing and modification.
Claims (10)
1. a kind of lactic acid bacteria and the composition of raw materials of saccharomycete symbiotic fermentation Sparassis crispa stoste, it is characterised in that including following weight
The raw material of number：60 parts~80 parts of Sparassis crispa extracting solution, 20 parts~40 parts of wheat embryo extracting solution, flavouring 0.1 part~0.5
Part, 0.01 part~0.03 part of 0.01 part~0.03 part of lactic acid bacteria and saccharomycete.
2. lactic acid bacteria according to claim 1 and the composition of raw materials of saccharomycete symbiotic fermentation Sparassis crispa stoste, its feature exist
In including the raw materials of following parts by weight：60 parts of Sparassis crispa extracting solution, 40 parts of wheat embryo extracting solution, 0.1 part of flavouring, breast
0.01 part of 0.03 part of sour bacterium and saccharomycete.
3. lactic acid bacteria according to claim 1 and the composition of raw materials of saccharomycete symbiotic fermentation Sparassis crispa stoste, its feature exist
In including the raw materials of following parts by weight：80 parts of Sparassis crispa extracting solution, 20 parts of wheat embryo extracting solution, 0.5 part of flavouring, breast
0.03 part of 0.01 part of sour bacterium and saccharomycete.
4. lactic acid bacteria according to claim 1 and the composition of raw materials of saccharomycete symbiotic fermentation Sparassis crispa stoste, its feature exist
In including the raw materials of following parts by weight：70 parts of Sparassis crispa extracting solution, 30 parts of wheat embryo extracting solution, 0.25 part of flavouring, breast
0.02 part of 0.02 part of sour bacterium and saccharomycete.
5. according to the composition of raw materials of claim 1-4 any one of them lactic acid bacteria and saccharomycete symbiotic fermentation Sparassis crispa stoste,
It is characterized in that, the lactic acid bacteria include at least lactobacillus paracasei, streptococcus thermophilus, lactobacillus plantarum, Lactobacillus rhamnosus,
Two kinds in newborn Bifidobacterium Bifidum, lactobacillus acidophilus, Lactobacillus casei, bifidobacterium adolescentis, the long double dry bacterium of qi and the short dry bacterium of double qis.
6. according to the composition of raw materials of claim 1-4 any one of them lactic acid bacteria and saccharomycete symbiotic fermentation Sparassis crispa stoste,
It is characterized in that, the saccharomycete includes at least one kind in brewer's yeast and Saccharomyces cerevisiae.
7. according to the composition of raw materials of claim 1-4 any one of them lactic acid bacteria and saccharomycete symbiotic fermentation Sparassis crispa stoste,
It is characterized in that, the flavouring is at least one kind in citric acid, malic acid and xylitol.
8. claim 1-7 any one of them lactic acid bacteria and the method for saccharomycete symbiotic fermentation Sparassis crispa stoste, its feature exist
In including the following steps：
S101：Sparassis crispa grind into powder is subsequently placed in Chinese medicine extracting tank, is 1 according still further to the weight ratio of Sparassis crispa and water：
(10~20) water is added, 2.5h~3.5h is then extracted at a temperature of 80 DEG C~100 DEG C, is then centrifuged for separating and filters, then receive
Collect filtrate, obtain Sparassis crispa extracting solution；
S102：Wheat embryo is placed in Chinese medicine extracting tank, is 1 according still further to the weight ratio of wheat embryo and water：(5~8) add
Water simultaneously stirs, and 2.5h~3.5h is then extracted at a temperature of 60 DEG C~75 DEG C, is then centrifuged for separating and filters, regather filtrate,
Obtain wheat embryo extracting solution；
S103：By the Sparassis crispa extracting solution and the wheat embryo extracting solution mix and be concentrated into relative density for 1.02~
1.04, it is subsequently placed in fermentation tank, adds the flavouring and stir evenly, then adds the citric acid and adjust its pH value
For 5.5~6.5；
S104：3min~8min is stood after the saccharomycete is mixed with the product of the step S103, is stirred for, then stands
30min~2h；
S105：The lactic acid bacteria is added into the product of the step S104 and is stirred, is then issued in 25 DEG C~35 DEG C temperature
Ferment 48h~72h, then filters and collects filtrate, then the filtrate is sterilized, and obtains lactic acid bacteria and the symbiotically fermented embroidery of saccharomycete
Coccus stoste.
9. lactic acid bacteria according to claim 7 and the method for saccharomycete symbiotic fermentation Sparassis crispa stoste, it is characterised in that
It is the mesh of 180 mesh~220 by the size of the Sparassis crispa powder in the step S101.
10. lactic acid bacteria according to claim 7 and the method for saccharomycete symbiotic fermentation Sparassis crispa stoste, it is characterised in that
In the step S105, sterilising temp is 110 DEG C~120 DEG C, and sterilization time is 20min~30min.
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