CN107969332B - Rapid induction of adventitious buds in rhizomes of velvet arrowroot - Google Patents

Abstract

The velvet arrowroot rhizome adventitious bud rapid induction method belongs to the field of flower propagation technology. Make an incision with a depth of 0.2 cm to 0.5 cm; use a cell crusher to make the sap, centrifuge it on a centrifuge for 10 to 20 minutes, and take the supernatant of the sage for later use; the obtained Velvet arrowroot rhizomes were put into the intermittent submerged plant incubator; the supernatant of the narrow-leaf bottle was diluted with purified water and injected into the intermittent submerged plant incubator, and the immersion frequency of the intermittent submerged plant incubator was set to every 1 Hourly immersion for 10 to 15 minutes, and the incubation temperature is set between 20°C and 30°C. The invention effectively solves the problems of long generation time and small number of adventitious buds in the process of velvet arrowroot rhizome cutting propagation.

Description

Rapid induction of adventitious buds in rhizomes of velvet arrowroot

technical field

The invention belongs to the technical field of flower propagation, and in particular relates to a method for rapidly inducing adventitious buds when promoting rhizome propagation of velvet arrowroot.

Background technique

Velvet arrowroot (Calathea leopardina) is a perennial evergreen herbaceous foliage plant of the arrowroot family. It is difficult to obtain seeds of arrowroot velvet, and the propagation methods that have been studied and applied are mainly tissue culture and rhizome cutting propagation.

Because of its high production cost and complicated operation, tissue culture is mainly used for laboratory research, and is rarely used by flower farmers in production. At present, the most widely used in production is still using its rhizomes for cutting propagation.

Disadvantages of the existing rhizome cutting propagation method: long culture period and low propagation coefficient. The main reason is that rooting is fast, but the formation of adventitious buds is very slow, and some leaf cuttings have not formed adventitious buds after half a year of rooting, that is, new complete plants cannot be formed. Moreover, the number of adventitious buds formed by a single cutting is small, basically about 1 to 3, which affects the ornamental effect of the plant.

SUMMARY OF THE INVENTION

In view of the deficiencies and defects of the prior art, the present invention provides a method for rapidly inducing adventitious buds of rhizomes of arrowroot velvet, so as to solve the problem that adventitious buds take a long time to form after rooting and propagation of rhizomes of velvet arrowroot, it is difficult to form new plants, and the number of adventitious buds occurs. less problems.

In order to solve the above-mentioned technical problems, the present invention realizes the method:

Velvet arrowroot rhizome adventitious bud rapid induction method, is characterized in that, comprises the following steps:

Step 1, take the material, cut the velvet arrowroot rhizome from the mother plant, cut it into a length of 3 cm to 6 cm, and use a knife to draw an incision of 0.2 cm to 0.5 cm deep on its epidermis every 1 cm;

Step 2, select the narrow-leaf bottle grass, utilize the cell crusher to make the narrow-leaf bottle grass into juice, centrifuge on the centrifuge for 10 minutes to 20 minutes, and get the narrow-leaf bottle grass supernatant for subsequent use;

Step 3, put the velvet arrowroot rhizome obtained in the step 1 into the intermittent immersion plant cultivator, while the narrow-leaf bottle grass supernatant liquid obtained in the step 2 is diluted with purified water and injected into the intermittent immersion plant cultivator. , wherein the weight ratio of the supernatant of the narrow-leaf bottle'er grass to the purified water is 1:(4～5);

Step 4. After steps 1 to 3 are completed, the immersion frequency of the intermittent immersion plant incubator is set to be immersed for 10 to 15 minutes every 1 hour, and the culture temperature is set to be between 20°C and 30°C.

Preferably, in step 3, the weight ratio of the supernatant of the narrow-leaf bottle'er grass to the purified water is 1:4.

Preferably, in step 3, the weight ratio of the supernatant of the narrow-leaf bottle'er grass to the purified water is 1:5.

Preferably, in step 4, the culture temperature is set to a constant temperature of 25°C.

Preferably, in step 4, the culture temperature is set to be cultured at a high temperature of 27°C for 12 hours and at a low temperature of 20°C for 12 hours every day.

Through the above-mentioned design scheme, the present invention can bring the following beneficial effects: the present invention effectively solves the problems of long time and few adventitious buds in the rhizome cutting propagation process of velvet arrowroot, and is beneficial to improve the reproductive efficiency of velvet arrowroot and commercial flowers quality.

Detailed ways

In order to illustrate the present invention more clearly, the present invention will be further described below with reference to the preferred embodiments. Those skilled in the art should understand that the content specifically described below is illustrative rather than restrictive, and should not limit the protection scope of the present invention.

The velvet arrowroot rhizome adventitious bud rapid induction method proposed by the present invention comprises the following steps:

Step 1, take the material, cut the velvet arrowroot rhizome from the mother plant, cut it into a length of 3 cm to 6 cm, and use a knife to draw an incision of 0.2 cm to 0.5 cm deep on its epidermis every 1 cm;

Step 2, select the narrow-leaf bottle grass, utilize the cell crusher to make the narrow-leaf bottle grass into juice, centrifuge on the centrifuge for 10 minutes to 20 minutes, and get the narrow-leaf bottle grass supernatant for subsequent use;

Step 3, put the velvet arrowroot rhizome obtained in the step 1 into the intermittent immersion plant cultivator, while the narrow-leaf bottle grass supernatant liquid obtained in the step 2 is diluted with purified water and injected into the intermittent immersion plant cultivator. , wherein the weight ratio of the supernatant of the narrow-leaf bottle'er grass to the purified water is 1:(4～5);

Step 4. After steps 1 to 3 are completed, the immersion frequency of the intermittent immersion plant incubator is set to be immersed for 10 to 15 minutes every 1 hour, that is, the velvet arrowroot rhizome will be in the narrow-leaf bottle's grass liquid every 1 hour. Immerse for 10 to 15 minutes, and set the incubation temperature between 20°C and 30°C.

Test results: After 15 to 20 days, obvious callus appeared on the surface of the rhizome of velvet arrowroot, and after 30 to 40 days, adventitious buds sprouted on the surface of the rhizome. At 60 days, more than 10 adventitious buds were basically formed among the 30 to 50 rhizomes in the statistical test.

Example 1

Cut the velvet arrowroot rhizome from the mother plant, cut it into a length of 3 cm to 4 cm, and use a knife to make a 0.3 cm deep incision every 1 cm on the epidermis; The narrow leaf bottle grass is broken with cells After the machine is made into juice, centrifuge on the centrifuge for 10 minutes, and take the supernatant of the narrow-leaf bottle of grass for use;

Put the velvet arrowroot rhizome into the intermittent immersion plant incubator; after diluting the supernatant of the narrow-leaf bottle of grass with purified water to obtain the narrow-leaf bottle of grass liquid, the weight of the narrow-leaf bottle of grass supernatant and purified water The ratio is 1:5, the narrow-leaf bottle grass liquid is injected into the intermittent submerged plant incubator, and the submersion frequency of the intermittent submerged plant incubator is set to be immersed for 10 minutes every 1 hour, that is, the velvet arrowroot rhizome will be immersed every 1 hour. Immerse in the liquid of the narrow-leaf bottle of grass for 10 minutes, and the culture temperature is constant temperature of 25 ℃.

Test results: After 17 days, obvious callus appeared on the surface of the rhizome of arrowroot velvet, and after 42 days, adventitious buds emerged on the surface of the rhizome. At 60 days, 30 rhizomes were counted, and an average of 12 adventitious buds were formed.

Example 2

Cut the rhizome of velvet arrowroot from the mother plant, cut it into a length of 5 cm to 6 cm, and use a knife to make a 0.5 cm deep incision on the epidermis every 1 cm; break the narrow-leaf bottle grass with cells After the machine is made into juice, centrifuge it on the centrifuge for 20 minutes, and take the supernatant of the narrow-leaf bottle of grass for use;

Put the velvet arrowroot rhizome into the intermittent immersion plant incubator; after diluting the supernatant of the narrow-leaf bottle of grass with purified water to obtain the narrow-leaf bottle of grass liquid, the weight of the narrow-leaf bottle of grass supernatant and purified water The ratio is 1:4, the narrow-leaf bottle grass liquid is injected into the intermittent submerged plant incubator, and the submersion frequency of the intermittent submerged plant incubator is set to be immersed for 15 minutes every 1 hour. Immerse in the liquid of the narrow-leaf bottle'er grass for 15 minutes, and the culture temperature is 12 hours at high temperature at 27°C and 12 hours at low temperature at 20°C.

Test results: After 15 days, obvious callus appeared on the surface of the rhizome of arrowroot velvet, and after 38 days, adventitious buds emerged on the surface of the rhizome. At 60 days, 50 cultured rhizomes were counted, and 18 adventitious buds were formed on average.

Obviously, the above-mentioned embodiments of the present invention are only examples for clearly illustrating the present invention, rather than limiting the implementation of the present invention. For other variations or changes in different forms, it is impossible to list all the embodiments here, and all obvious changes or changes derived from the technical solutions of the present invention are still within the protection scope of the present invention.

Claims (3)

1. velvet arrowroot rhizome adventitious bud rapid induction method, is characterized in that, comprises the following steps: Step 1, take the material, cut the velvet arrowroot rhizome from the mother plant, cut it into a length of 3 cm to 6 cm, and use a knife to draw an incision of 0.2 cm to 0.5 cm deep on its epidermis every 1 cm; Step 2, select the narrow-leaf bottle grass, utilize the cell crusher to make the narrow-leaf bottle grass into juice, centrifuge on the centrifuge for 10 minutes to 20 minutes, and get the narrow-leaf bottle grass supernatant for subsequent use; Step 3, put the velvet arrowroot rhizome obtained in the step 1 into the intermittent immersion plant cultivator, while the narrow-leaf bottle grass supernatant liquid obtained in the step 2 is diluted with purified water and injected into the intermittent immersion plant cultivator. , wherein the weight ratio of the supernatant of the narrow-leaf bottle'er grass to the purified water is 1:(4～5); Step 4. After steps 1 to 3 are completed, the immersion frequency of the intermittent immersion plant incubator is set to be immersed for 10 minutes to 15 minutes every 1 hour, and the culture temperature is set to be between 20°C and 30°C; The culture temperature was set at a constant temperature of 25°C or at a high temperature of 27°C for 12 hours and a low temperature of 20°C for 12 hours every day. 2. velvet arrowroot rhizome adventitious bud rapid induction method according to claim 1, is characterized in that: described in step 3, narrow-leaf bottle's grass supernatant and purified water weight ratio are 1:4. 3. velvet arrowroot rhizome adventitious bud rapid induction method according to claim 1, is characterized in that: described in step 3, narrow-leaf bottle's grass supernatant and purified water weight ratio are 1:5.