CN107969332B - Fast inducing method for adventitious bud of velvet arrowroot - Google Patents
Fast inducing method for adventitious bud of velvet arrowroot Download PDFInfo
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- CN107969332B CN107969332B CN201711205693.8A CN201711205693A CN107969332B CN 107969332 B CN107969332 B CN 107969332B CN 201711205693 A CN201711205693 A CN 201711205693A CN 107969332 B CN107969332 B CN 107969332B
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
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Abstract
A rapid induction method of adventitious buds of rootstock of calathea velvet arundinacea belongs to the technical field of flower reproduction, and is characterized in that the rootstock of calathea velvet arundinacea is cut off from a stock plant, the length of the calathea velvet arundinacea is cut into 3-6 cm, and a cut with the depth of 0.2-0.5 cm is cut on the surface of the calathea velvet arundinacea every 1 cm by a knife; beating the small lophatherum angustifolium into juice by using a cell crusher, centrifuging the juice for 10 to 20 minutes on a centrifugal machine, and taking the supernatant of the small lophatherum angustifolium for later use; putting the obtained rootstocks of the velvet arrowroot into an intermittent immersion type plant culture device; diluting the supernatant of the small grass of the stenotropha with purified water, injecting the diluted supernatant into an intermittent immersion type plant incubator, and setting the immersion frequency of the intermittent immersion type plant incubator to be 10-15 minutes of immersion every 1 hour, wherein the culture temperature is set to be 20-30 ℃. The invention effectively solves the problems of long time and small quantity of adventitious buds in the cutting propagation process of the rhizomes of the velvet arrowroot.
Description
Technical Field
The invention belongs to the technical field of flower propagation, and particularly relates to a rapid adventitious bud induction method for promoting rhizome propagation of velvet arrowroot.
Background
Calathea leopardus (Calathea leopardina) is a perennial evergreen herbaceous foliage plant of the genus Schoenoprass of the family Arathnaceae. Seeds are difficult to obtain from the velvet arrowroot, and the currently researched and applied propagation method mainly comprises tissue culture and rhizome cutting propagation.
Tissue culture is mainly used for laboratory research because of its high production cost and complex operation, and is less adopted by flower growers in production. At present, the rootstocks of the plants are still used for cutting propagation in more applications in production.
The existing rhizome cutting propagation method has the following defects: long culture period and low propagation coefficient. The main reasons are: the rooting is fast, but the adventitious bud is formed very slowly, and the adventitious bud is not formed after the cutting shoot of some leaves roots for half a year, namely a new complete plant cannot be formed. And the number of adventitious buds formed by a single cutting shoot is small, basically about 1-3, and the ornamental effect of plants is influenced.
Disclosure of Invention
Aiming at the defects of the prior art, the invention provides a rapid induction method of adventitious buds of rhizomes of calathea velveteensis, which aims to solve the problems that the adventitious buds are difficult to form new plants and the number of the adventitious buds is small after the rhizomes of calathea velveteensis are rooted by cutting propagation.
In order to solve the technical problem, the implementation method of the invention comprises the following steps:
the method for rapidly inducing the adventitious buds of the rhizomes of the velvet arrowroot is characterized by comprising the following steps:
firstly, taking materials, cutting off rhizomes of velvet arrowroot from a stock plant, cutting the rhizomes into 3-6 cm long, and cutting a cut with the depth of 0.2-0.5 cm on the surface of the rhizomes every 1 cm by using a knife;
step two, selecting small narrow-leaved pitcher herb, beating the small narrow-leaved pitcher herb into juice by using a cell crusher, centrifuging the juice for 10 to 20 minutes on a centrifugal machine, and taking supernatant of the small narrow-leaved pitcher herb for later use;
step three, putting the rootstocks of the velvet arrowroot obtained in the step one into an intermittent immersion type plant culture device, diluting the supernatant of the small grass in the step two by using purified water, and injecting the diluted supernatant into the intermittent immersion type plant culture device, wherein the weight ratio of the supernatant of the small grass in the narrow leaf to the purified water is 1 (4-5);
step four, after the step one to the step three are completed, the immersion frequency of the intermittent immersion type plant incubator is set to be 10 minutes to 15 minutes of immersion every 1 hour, and the culture temperature is set to be 20 ℃ to 30 ℃.
Preferably, the weight ratio of the small grass of stenotropha supernatant to the purified water in the third step is 1: 4.
Preferably, the weight ratio of the small grass of stenotropha supernatant to the purified water in the third step is 1: 5.
Preferably, in the fourth step, the temperature of the culture is set to a constant temperature of 25 ℃.
Preferably, in the fourth step, the culture temperature is set to 27 ℃ for 12 hours at a high temperature and 20 ℃ for 12 hours at a low temperature per day.
Through the design scheme, the invention can bring the following beneficial effects: the method effectively solves the problems of long time and small quantity of adventitious buds in the rootstock cutting propagation process of the velvet arrowroot, and is favorable for improving the propagation efficiency of the velvet arrowroot and the quality of commodity flowers.
Detailed Description
In order to more clearly illustrate the invention, the invention is further described below in connection with preferred embodiments. It is to be understood by persons skilled in the art that the following detailed description is illustrative and not restrictive, and is not to be taken as limiting the scope of the invention.
The invention provides a rapid induction method for adventitious buds of rhizomes of arrowroot of velvet, which comprises the following steps:
firstly, taking materials, cutting off rhizomes of velvet arrowroot from a stock plant, cutting the rhizomes into 3-6 cm long, and cutting a cut with the depth of 0.2-0.5 cm on the surface of the rhizomes every 1 cm by using a knife;
step two, selecting small narrow-leaved pitcher herb, beating the small narrow-leaved pitcher herb into juice by using a cell crusher, centrifuging the juice for 10 to 20 minutes on a centrifugal machine, and taking supernatant of the small narrow-leaved pitcher herb for later use;
step three, putting the rootstocks of the velvet arrowroot obtained in the step one into an intermittent immersion type plant culture device, diluting the supernatant of the small grass in the step two by using purified water, and injecting the diluted supernatant into the intermittent immersion type plant culture device, wherein the weight ratio of the supernatant of the small grass in the narrow leaf to the purified water is 1 (4-5);
step four, after the step one to the step three are completed, the immersion frequency of the intermittent immersion type plant incubator is set to be 10 minutes to 15 minutes of immersion every 1 hour, namely, the rhizomes of the velvet arrowroot can be immersed in the small grass liquid of the narrow-leaved pitcher for 10 minutes to 15 minutes every 1 hour, and the culture temperature is set to be between 20 ℃ and 30 ℃.
And (3) test results: obvious callus appears on the surface of the rootstock of the velvet arrowroot after 15 to 20 days, adventitious buds sprout on the surface of the rootstock after 30 to 40 days, and 30 to 50 rootstock are counted and tested at 60 days to basically form more than 10 adventitious buds.
Example 1
Cutting off rootstocks of velvet arrowroots from a stock plant, cutting the rootstocks into cuts with the length of 3-4 cm, and cutting a cut with the depth of 0.3 cm on the surface of the cut every 1 cm by a cutter; beating the small lophatherum angustifolium into juice by using a cell crusher, centrifuging the juice for 10 minutes on a centrifugal machine, and taking the supernatant of the small lophatherum angustifolium for later use;
putting the rootstocks of the velvet arrowroot into an intermittent immersion type plant culture device; diluting the small tulip grass supernatant with purified water to obtain small tulip grass liquid, wherein the weight ratio of the small tulip grass supernatant to the purified water is 1:5, injecting the small tulip grass liquid into an intermittent immersion type plant culture device, and setting the immersion frequency of the intermittent immersion type plant culture device to be 10 minutes of immersion every 1 hour, namely, the rootstocks of the velvet arrowroot can be immersed in the small tulip grass liquid for 10 minutes every 1 hour, and the culture temperature is 25 ℃.
And (3) test results: obvious callus appears on the surface of the rootstock of the velvet arrowroot after 17 days, adventitious buds sprout on the surface of the rootstock after 42 days, 30 cultured rootstocks are counted at 60 days, and 12 adventitious buds are formed on average.
Example 2
Cutting off rootstocks of velvet arrowroots from a stock plant, cutting the rootstocks into 5-6 cm long, and cutting a cut with the depth of 0.5 cm on the surface of the rootstocks by a cutter every 1 cm; beating the small lophatherum angustifolium into juice by using a cell crusher, centrifuging the juice for 20 minutes on a centrifugal machine, and taking the supernatant of the small lophatherum angustifolium for later use;
putting the rootstocks of the velvet arrowroot into an intermittent immersion type plant culture device; diluting the small lophatherum gracile supernatant with purified water to obtain small lophatherum gracile liquid, wherein the weight ratio of the small lophatherum gracile supernatant to the purified water is 1:4, injecting the small lophatherum gracile liquid into an intermittent immersion type plant culture device, and setting the immersion frequency of the intermittent immersion type plant culture device to be 15 minutes of immersion every 1 hour, namely immersing the rhizomes of the velvet arrowroot in the small lophatherum gracile liquid for 15 minutes every 1 hour, wherein the culture temperature is 27 ℃ per day for 12 hours, and the culture temperature is 20 ℃ per day for 12 hours.
And (3) test results: obvious callus appears on the surface of the rootstock of the velvet arrowroot after 15 days, adventitious buds sprout on the surface of the rootstock after 38 days, 50 cultured rootstocks are counted at 60 days, and 18 adventitious buds are formed on average.
It should be understood that the above-mentioned embodiments of the present invention are only examples for clearly illustrating the present invention, and are not intended to limit the embodiments of the present invention, and it will be obvious to those skilled in the art that other variations and modifications can be made on the basis of the above description, and all embodiments cannot be exhaustive, and obvious variations and modifications may be made within the scope of the present invention.
Claims (3)
1. The method for rapidly inducing the adventitious buds of the rhizomes of the velvet arrowroot is characterized by comprising the following steps:
firstly, taking materials, cutting off rhizomes of velvet arrowroot from a stock plant, cutting the rhizomes into 3-6 cm long, and cutting a cut with the depth of 0.2-0.5 cm on the surface of the rhizomes every 1 cm by using a knife;
step two, selecting small narrow-leaved pitcher herb, beating the small narrow-leaved pitcher herb into juice by using a cell crusher, centrifuging the juice for 10 to 20 minutes on a centrifugal machine, and taking supernatant of the small narrow-leaved pitcher herb for later use;
step three, putting the rootstocks of the velvet arrowroot obtained in the step one into an intermittent immersion type plant culture device, diluting the supernatant of the small grass in the step two by using purified water, and injecting the diluted supernatant into the intermittent immersion type plant culture device, wherein the weight ratio of the supernatant of the small grass in the narrow leaf to the purified water is 1 (4-5);
step four, after the step one to the step three are completed, the immersion frequency of the intermittent immersion type plant incubator is set to be 10 minutes to 15 minutes of immersion every 1 hour, and the culture temperature is set to be between 20 ℃ and 30 ℃;
the culture temperature is set to be constant at 25 ℃ or set to be high at 27 ℃ for 12 hours and low at 20 ℃ for 12 hours per day.
2. The rapid induction method of adventitious bud of a rootstock of calathea velveteen according to claim 1, characterized in that: in the third step, the weight ratio of the small grass of stenotropha supernatant to the purified water is 1: 4.
3. The rapid induction method of adventitious bud of a rootstock of calathea velveteen according to claim 1, characterized in that: in the third step, the weight ratio of the small grass of stenotropha supernatant to the purified water is 1: 5.
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2008093526A (en) * | 2006-10-06 | 2008-04-24 | Shigetoshi Mizusawa | Manufacturing method of active water |
| US8067673B2 (en) * | 2004-08-20 | 2011-11-29 | Maharashtra Hybrid Seeeds Company Ltd. | Methods for plant regeneration, transformation and production of insect resistant transgenic Okra |
| CN103636499A (en) * | 2013-12-05 | 2014-03-19 | 长春大学 | Rapid induction method for inserting adventitious bud on bean halve green leaf |
| CN103931501A (en) * | 2014-04-29 | 2014-07-23 | 长春大学 | Method for effectively producing protocorm by using zamiolulcas zamiif olia compound leaf rachis |
| CN105052500A (en) * | 2015-08-20 | 2015-11-18 | 中国科学院东北地理与农业生态研究所 | Method for rapid propagation of switchgrass through rhizome transplantation |
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Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US8067673B2 (en) * | 2004-08-20 | 2011-11-29 | Maharashtra Hybrid Seeeds Company Ltd. | Methods for plant regeneration, transformation and production of insect resistant transgenic Okra |
| JP2008093526A (en) * | 2006-10-06 | 2008-04-24 | Shigetoshi Mizusawa | Manufacturing method of active water |
| CN103636499A (en) * | 2013-12-05 | 2014-03-19 | 长春大学 | Rapid induction method for inserting adventitious bud on bean halve green leaf |
| CN103931501A (en) * | 2014-04-29 | 2014-07-23 | 长春大学 | Method for effectively producing protocorm by using zamiolulcas zamiif olia compound leaf rachis |
| CN105052500A (en) * | 2015-08-20 | 2015-11-18 | 中国科学院东北地理与农业生态研究所 | Method for rapid propagation of switchgrass through rhizome transplantation |
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