CN107964053A - A kind of extracting method of Shengmai Yin polysaccharide - Google Patents
A kind of extracting method of Shengmai Yin polysaccharide Download PDFInfo
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- CN107964053A CN107964053A CN201810065145.8A CN201810065145A CN107964053A CN 107964053 A CN107964053 A CN 107964053A CN 201810065145 A CN201810065145 A CN 201810065145A CN 107964053 A CN107964053 A CN 107964053A
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- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
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Abstract
The present invention provides a kind of extracting method of Shengmai Yin polysaccharide, it includes the following steps：A, bulk pharmaceutical chemicals are weighed：Red ginseng, ophiopogon, Schisandra chinensis, crush；B, water carries；C, alcohol precipitation；D, wash, obtain polyoses extract.Present invention also offers the polyoses extract that extraction obtains.The method of the present invention can be with the polysaccharide in high efficiency extraction Shengmai Yin prescription, obtained polysaccharide extract rate antioxidation is strong, and stability is high, and theoretical foundation is provided for the research and development and quality control of Shengmai Yin preparation, and the method for the present invention simple possible, it is adapted to large-scale industrial production.
Description
Technical field
The invention belongs to technical field of extraction of Chinese traditional medicine, and in particular to a kind of extracting method of Shengmai Yin polysaccharide.
Background technology
Ancient times classics recipe " Shengmai San ", also known as Shengmai Yin, first recorded in written by Jin and Yuan dynasty Zhang Yuansu《Medicine origin》
(1186), shape in《Differentiation on endogenous》, improve in《Hospital examines》.In classics are formulated, by ginseng 1g, ophiopogon 2g, five
Tri- taste medicines of taste 1g form.Function：Nourishing generate fluid, astringing YIN to stop sweating.Cure mainly:1. the lung being damaged by lasted cough, deficiency of both qi and yin disease.Dry cough lacks phlegm,
Lose heart spontaneous perspiration, mouth parched and tongue scorched, deficient and thready pulse；2. hot summer weather gas consumption impairment of yin disease.More refreshing tired, the fatigue and asthenias of sweat, the lazy speech of shortness of breath, dry throat mouth
Yearningly, tongue extra dry red wine lacks tongue, and arteries and veins carrys out imaginary number.
Shengmai Yin is used till today always due to evident in efficacy, modern pharmacological studies have shown that Shengmai Yin have expansion blood vessel,
Cardiac muscle cell, increase coronary flow are protected, blood pressure is adjusted, improve immune function, strengthen anti-oxidant, anticancer and suppresses virus
Deng effect.People develop Shengmai Yin, raw arteries and veins piece, raw pulse agent, raw arteries and veins bagged instant preparation, life in succession according to different needs of medical treatment
Arteries and veins capsule, raw arteries and veins oral liquid, a variety of formulations of raw arteries and veins syrup and shengmai injection etc..
Research shows that clinical efficacy and the polysaccharide constituents of Shengmai Yin preparation have substantial connection, and polysaccharide, which has, to be significantly improved
The immunologic enhancement such as macrophage index and E garland formation rates and the effect of a variety of pharmacological activity, Gu Rencan, ophiopogon, Schisandra chinensis
In polysaccharide one of for principle active component.The higher extraction method of polysaccharides of recovery rate is explored, and further studies its pharmacology work
With the preparation process optimization and clinical efficacy raising to Shengmai Yin are significant.
The content of the invention
Extracting method and the polyoses extract that is prepared it is an object of the invention to provide a kind of Shengmai Yin polysaccharide.
The present invention provides a kind of extracting method of Shengmai Yin polysaccharide, it includes the following steps：
A, bulk pharmaceutical chemicals are weighed：Red ginseng, ophiopogon, Schisandra chinensis, crush；
B, water carries：In the powder of step a, it is 8-10g/mL to add water to solid-liquid ratio, at 80-90 DEG C, extracts 30-
60min, is extracted 1-3 times；
C, alcohol precipitation：Extracting solution is concentrated, adds ethanol, is stood, collects precipitation；
D, wash：Precipitation is washed with ethanol solution, acetone and ether successively, it is dry, obtain polyoses extract.
Wherein, in step a, the proportioning of bulk pharmaceutical chemicals is：0.7-1.3 parts of red ginseng, ophiopogon 1.4-2.6 part, Schisandra chinensis 0.7-1.3
Part.
Wherein, the proportioning of bulk pharmaceutical chemicals is：1 part of red ginseng, 2 parts of ophiopogon, 1 part of Schisandra chinensis.
Wherein, in step b, the time of extraction is 45-60min, is extracted 1 time.
Wherein, in step b, the condition that water carries is：It is 10g/mL to add water to solid-liquid ratio, at 80 DEG C, extracts 60min, carries
Take 1 time.
Wherein, in step c, the concentration of ethanol is 95%.
Wherein, in step c, the mode of standing is：At 4 DEG C overnight.
Wherein, in step d, the number of washing is 2 times.
Wherein, in step d, dry temperature is 60 DEG C.
Present invention also offers the polyoses extract that said extracted method is prepared.
The method of the present invention can be with the polysaccharide in high efficiency extraction Shengmai Yin prescription, and can fully ensure that the activity of polysaccharide, grinds
Study carefully discovery, polyoses extract of the present invention has good antioxidation, be the research and development of Shengmai Yin preparation, quality control,
Pharmacological research provides theoretical foundation；And the method for the present invention simple possible, it is adapted to large-scale industrial production.
Obviously, the above according to the present invention, according to the ordinary technical knowledge and customary means of this area, is not departing from
Under the premise of the above-mentioned basic fundamental thought of the present invention, the modification, replacement or change of other diversified forms can also be made.
The embodiment of form by the following examples, remakes further specifically the above of the present invention
It is bright.But the scope that this should not be interpreted as to the above-mentioned theme of the present invention is only limitted to following example.It is all to be based on the above of the present invention
The technology realized belongs to the scope of the present invention.
Brief description of the drawings
The ability that Fig. 1 Shengmai Yin polysaccharide of the present invention removes DPPH free radicals is investigated
The acid resistance of Fig. 2 Shengmai Yin polysaccharide scavenging ability of DPPH free radical of the present invention is investigated
The heat-resisting quantity of Fig. 3 Shengmai Yin polysaccharide scavenging ability of DPPH free radical of the present invention is investigated
The storage-stable of Fig. 4 Shengmai Yin polysaccharide scavenging ability of DPPH free radical of the present invention is investigated
Embodiment
It is described further below with embodiment, but the present invention is not limited to these embodiments.
Experiment reagent and instrument used in the present invention is as follows：
Experiment reagent：95% ethanol, absolute ethyl alcohol (analysis is pure), anhydrous ether, acetone, sulfuric acid, anthrone, it is commercially available.
Laboratory apparatus：TU-1810 ultraviolet-visible spectrophotometers (Beijing Puxi General Instrument Co., Ltd), rotation
Turn evaporimeter, Alleregra X30R multifunctional table-types high speed freezing centrifuge (Beckman Kurt commerce and trade Co., Ltd).
1 extracting method of the present invention of embodiment
Weigh red ginseng, ophiopogon and Schisandra chinensis (1:2:1) powder is beaten, is put into erlenmeyer flask, according to solid-liquid ratio 1:10(g/ml)
Extracting in water, extraction time 60min, 80 DEG C of Extracting temperature, extraction time 1 time, obtains extracting solution.
To extracting solution, filtering, is concentrated into the 17% of original volume, concentrate adds 95% second of 4 times of volumes with low-temperature evaporation
Alcoholic solution, stands overnight in 4 DEG C of refrigerators, filters, and washs precipitation 2 times, 60 DEG C with ethanol solution, acetone and ether successively
Dry Thick many candies composition.
Beneficial effects of the present invention are illustrated below by way of test example：
The screening of 1 extracting method of test example
1st, Study on extraction
Polysaccharide is extracted using water extraction and alcohol precipitation method.
Weigh red ginseng, ophiopogon and Schisandra chinensis (1:2:1) powder is beaten, is put into erlenmeyer flask, different feed liquid ratio is set, in difference
Different time, different extraction times are extracted under Extracting temperature, probes into the optimised process of Polyose extraction in Shengmai Yin.
To extracting solution, filter, the 17% of original volume is concentrated into low-temperature evaporation, concentrate adds 95% second of 4 times of volumes
Alcoholic solution, stands overnight in 4 DEG C of refrigerators, filters, and washs precipitation 2 times, 60 DEG C with ethanol solution, acetone and ether successively
Dry Thick many candies composition.
2nd, polysaccharide determination method
Polyoses content is measured using Phenol-sulphate acid method, the dry dextrose standard sample 10mg to constant weight is accurately weighed, is placed in
In volumetric flask, add distilled water to dissolve and be diluted to scale, up to the dextrose standard sample solution that concentration is 0.1mg/mL.Essence respectively
Really absorption 0.4,0.6,0.8,1.0,1.2,1.4,1.6,1.8mL dextrose standard sample solution are placed in centrifuge tube, are settled to
2ml, then newly formed sulfuric acid-anthrone solution 6ml is accurately added, boiling water bath 20min takes out, is put into cold water and cools down, to wait body
Ponding, as blank control, absorbance is surveyed using ultraviolet-uisible spectrophotometer by same color operation at 625nm wavelength.
It is that ordinate (y) draws standard curve using concentration of glucose as abscissa (x/mg/mL), absorbance, obtains the side of recurrence
Cheng Wei：Y=8.36x-0.042, R2=0.9993.
3rd, the measurement result of Different Extraction Method
It is shown in Table 1.
1 Shengmai Yin polysaccharide yield of table
| Numbering | Solid-liquid ratio/(gmL-1) | Extraction time/min | Extracting temperature/DEG C | Extraction time/(secondary) | Polysaccharide extract rate/% |
| 1 | 1:6 | 30 | 70 | 1 | 4.47 |
| 2 | 1:6 | 45 | 80 | 2 | 5.47 |
| 3 | 1:6 | 60 | 90 | 3 | 5.58 |
| 4 | 1:8 | 30 | 80 | 3 | 6.21 |
| 5 | 1:8 | 45 | 90 | 1 | 7.27 |
| 6 | 1:8 | 60 | 70 | 2 | 3.92 |
| 7 | 1:10 | 30 | 90 | 2 | 5.42 |
| 8 | 1:10 | 45 | 70 | 3 | 4.69 |
| 9 | 1:10 | 60 | 80 | 1 | 7.58 |
By table 1 as it can be seen that the extraction process that numbering is 4,5,9 has higher polysaccharide extract rate, wherein, the extraction of numbering 9
Best results, i.e. solid-liquid ratio 1:10, extraction time 60min, 80 DEG C of Extracting temperature, extraction time 1 time, gained polysaccharide extract rate is most
Height, is 7.58%.
The Antioxidation in vitro detection of 2 polyoses extract of the present invention of test example
1st, detection method
Using the ability for removing DPPH (hexichol generation bitter diazanyl free radical) free radical, the anti-oxidant work of Shengmai Yin polysaccharide is measured
Property.
The DPPH of 0.1mmol/L is pre-configured with, above-mentioned DPPH solution and 2.0mL samples are successively added in 10mL colorimetric cylinders
Solvent (distilled water), mix, stand 30min after, with cuvette at 490nm ripples, survey absorbance, be denoted as A0；Will raw arteries and veins
Drink polysaccharide composition dilutes 0.2 respectively using distilled water, 0.4,0.6,0.8,1.0,6 gradients such as 1.2mg/mL, addition
The solution and 2mL Shengmai Yin polysaccharide compositions sample of the DPPH of 2.0mL0.1mmol/L measures absorbance after mixing, and is denoted as Ai.
Each absorbance parallel determination 3 times, takes its average value.
Clearance rate is calculated as follows：Clearance rate=[(A0-Ai)/A0] × 100%, using sample concentration as abscissa,
Clearance rate is ordinate, establishes the relation of polysaccharide and DPPH free radical scavenging activities.
2nd, testing result
See Fig. 1.
As shown in Figure 1, the Shengmai Yin polysaccharide that the present invention extracts has good oxidation resistance, and polysaccharide is to DPPH free radicals
Scavenging action, gradually strengthen with the increase of polysaccharide concentration.
Then the influence of pH, temperature and time to Shengmai Yin polysaccharide solution oxidation resistance stability is investigated.Take concentration
PH 2,4,6,8,10,12 is adjusted respectively for the polysaccharide composition of 1mg/mL, after room temperature places 2h, measures it to DPPH free radicals
Clearance rate, the result is shown in Fig. 2.
Polysaccharide composition solution has been placed in lid centrifuge tube, respectively at heating 2h in 40,60,80,100 DEG C of water-baths, has been measured
Its clearance rate to DPPH free radicals, the result is shown in Fig. 3.
By polysaccharide composition solution room temperature 2,4,8,12,24h, its clearance rate to DPPH free radicals is measured, as a result
See Fig. 4.
From Fig. 2-4, the Shengmai Yin polysaccharide that the present invention extracts has acid resistance, heat-resisting quantity and storage-stable, because
This, oxidation resistance is stablized.
The method of the present invention can be with the polysaccharide in high efficiency extraction Shengmai Yin prescription, and can fully ensure that the activity of polysaccharide, grinds
Study carefully discovery, polyoses extract of the present invention has good antioxidation, be the research and development of Shengmai Yin preparation, quality control,
Pharmacological research provides theoretical foundation.And the method for the present invention simple possible, it is adapted to large-scale industrial production.
Claims (10)
- A kind of 1. extracting method of Shengmai Yin polysaccharide, it is characterised in that：It includes the following steps：A, bulk pharmaceutical chemicals are weighed：Red ginseng, ophiopogon, Schisandra chinensis, crush；B, water carries：In the powder of step a, it is 8-10g/mL to add water to solid-liquid ratio, at 80-90 DEG C, extracts 30-60min, carries Take 1-3 times；C, alcohol precipitation：Extracting solution is concentrated, adds ethanol, is stood, collects precipitation；D, wash：Precipitation is washed with ethanol solution, acetone and ether successively, it is dry, obtain polyoses extract.
- 2. extracting method according to claim 1, it is characterised in that：In step a, the proportioning of bulk pharmaceutical chemicals is：Red ginseng 0.7- 1.3 parts, ophiopogon 1.4-2.6 part, 0.7-1.3 parts of Schisandra chinensis.
- 3. extracting method according to claim 1 or 2, it is characterised in that：The proportioning of bulk pharmaceutical chemicals is：1 part of red ginseng, ophiopogon 2 Part, 1 part of Schisandra chinensis.
- 4. extracting method according to claim 1, it is characterised in that：In step b, the time of extraction is 45-60min, is carried Take 1 time.
- 5. according to the extracting method described in claim 1-4 any one, it is characterised in that：In step b, the condition that water carries is： It is 10g/mL to add water to solid-liquid ratio, at 80 DEG C, extracts 60min, extracts 1 time.
- 6. extracting method according to claim 1, it is characterised in that：In step c, the concentration of ethanol is 95%.
- 7. extracting method according to claim 1, it is characterised in that：In step c, the mode of standing is：At 4 DEG C overnight.
- 8. extracting method according to claim 1, it is characterised in that：In step d, the number of washing is 2 times.
- 9. extracting method according to claim 1, it is characterised in that：In step d, dry temperature is 60 DEG C.
- 10. the polyoses extract that extracting method described in claim 1-9 any one methods is prepared.
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1116894C (en) * | 1999-03-09 | 2003-08-06 | 中国药科大学 | Medicine for treating cardiovascular disease and preparation process thereof |
| CN101744986A (en) * | 2008-12-05 | 2010-06-23 | 天津天士力之骄药业有限公司 | Extraction method for ginseng, ophiopogon root and schisandra chinensis and preparation thereof |
| CN107308179A (en) * | 2016-04-27 | 2017-11-03 | 成都中医药大学 | A kind of monkshood licorice polysaccharide extract and preparation method thereof |
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2018
- 2018-01-23 CN CN201810065145.8A patent/CN107964053A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1116894C (en) * | 1999-03-09 | 2003-08-06 | 中国药科大学 | Medicine for treating cardiovascular disease and preparation process thereof |
| CN101744986A (en) * | 2008-12-05 | 2010-06-23 | 天津天士力之骄药业有限公司 | Extraction method for ginseng, ophiopogon root and schisandra chinensis and preparation thereof |
| CN107308179A (en) * | 2016-04-27 | 2017-11-03 | 成都中医药大学 | A kind of monkshood licorice polysaccharide extract and preparation method thereof |
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Application publication date: 20180427 |