CN107960470A - A kind of preparation method of Cordyceps militaris mushroom functional form fermented beverage - Google Patents
A kind of preparation method of Cordyceps militaris mushroom functional form fermented beverage Download PDFInfo
- Publication number
- CN107960470A CN107960470A CN201711231995.2A CN201711231995A CN107960470A CN 107960470 A CN107960470 A CN 107960470A CN 201711231995 A CN201711231995 A CN 201711231995A CN 107960470 A CN107960470 A CN 107960470A
- Authority
- CN
- China
- Prior art keywords
- parts
- cordyceps militaris
- zymotic fluid
- preparation
- medium
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 241001264174 Cordyceps militaris Species 0.000 title claims abstract description 103
- 235000001674 Agaricus brunnescens Nutrition 0.000 title claims abstract description 82
- 238000002360 preparation method Methods 0.000 title claims abstract description 57
- 235000019985 fermented beverage Nutrition 0.000 title claims abstract description 38
- 239000012530 fluid Substances 0.000 claims abstract description 119
- 238000000855 fermentation Methods 0.000 claims abstract description 90
- 230000004151 fermentation Effects 0.000 claims abstract description 90
- 239000007788 liquid Substances 0.000 claims abstract description 77
- 240000000599 Lentinula edodes Species 0.000 claims abstract description 50
- 235000001715 Lentinula edodes Nutrition 0.000 claims abstract description 48
- 241000894006 Bacteria Species 0.000 claims abstract description 29
- 235000013336 milk Nutrition 0.000 claims abstract description 26
- 239000008267 milk Substances 0.000 claims abstract description 26
- 210000004080 milk Anatomy 0.000 claims abstract description 26
- 239000000839 emulsion Substances 0.000 claims abstract description 21
- 239000002131 composite material Substances 0.000 claims abstract description 20
- 235000003599 food sweetener Nutrition 0.000 claims abstract description 20
- 239000003381 stabilizer Substances 0.000 claims abstract description 20
- 239000003765 sweetening agent Substances 0.000 claims abstract description 20
- 235000013960 Lactobacillus bulgaricus Nutrition 0.000 claims abstract description 19
- 241000194020 Streptococcus thermophilus Species 0.000 claims abstract description 19
- 150000001875 compounds Chemical class 0.000 claims abstract description 19
- 229940004208 lactobacillus bulgaricus Drugs 0.000 claims abstract description 19
- 239000002609 medium Substances 0.000 claims description 90
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims description 56
- 239000008103 glucose Substances 0.000 claims description 56
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 claims description 51
- 239000000706 filtrate Substances 0.000 claims description 50
- 230000004913 activation Effects 0.000 claims description 49
- 230000001954 sterilising effect Effects 0.000 claims description 42
- 239000001888 Peptone Substances 0.000 claims description 40
- 108010080698 Peptones Proteins 0.000 claims description 40
- 235000019319 peptone Nutrition 0.000 claims description 40
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Chemical compound O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 34
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 32
- 239000006143 cell culture medium Substances 0.000 claims description 32
- 239000001963 growth medium Substances 0.000 claims description 31
- 239000000463 material Substances 0.000 claims description 27
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 23
- 239000002054 inoculum Substances 0.000 claims description 22
- 235000019796 monopotassium phosphate Nutrition 0.000 claims description 22
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 claims description 22
- 210000000582 semen Anatomy 0.000 claims description 21
- 239000001814 pectin Substances 0.000 claims description 19
- 229920001277 pectin Polymers 0.000 claims description 19
- 235000010987 pectin Nutrition 0.000 claims description 19
- 239000006188 syrup Substances 0.000 claims description 19
- 235000020357 syrup Nutrition 0.000 claims description 19
- 229920000858 Cyclodextrin Polymers 0.000 claims description 18
- 239000001116 FEMA 4028 Substances 0.000 claims description 18
- 244000199885 Lactobacillus bulgaricus Species 0.000 claims description 18
- WHGYBXFWUBPSRW-FOUAGVGXSA-N beta-cyclodextrin Chemical compound OC[C@H]([C@H]([C@@H]([C@H]1O)O)O[C@H]2O[C@@H]([C@@H](O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O[C@H]3O[C@H](CO)[C@H]([C@@H]([C@H]3O)O)O3)[C@H](O)[C@H]2O)CO)O[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@@H]3O[C@@H]1CO WHGYBXFWUBPSRW-FOUAGVGXSA-N 0.000 claims description 18
- 235000011175 beta-cyclodextrine Nutrition 0.000 claims description 18
- 229960004853 betadex Drugs 0.000 claims description 18
- 238000001914 filtration Methods 0.000 claims description 18
- 235000010855 food raising agent Nutrition 0.000 claims description 18
- 235000021552 granulated sugar Nutrition 0.000 claims description 18
- 229920001817 Agar Polymers 0.000 claims description 16
- 241001248610 Ophiocordyceps sinensis Species 0.000 claims description 16
- 244000061456 Solanum tuberosum Species 0.000 claims description 16
- 235000002595 Solanum tuberosum Nutrition 0.000 claims description 16
- 229930006000 Sucrose Natural products 0.000 claims description 16
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 16
- 239000008272 agar Substances 0.000 claims description 16
- 235000015278 beef Nutrition 0.000 claims description 16
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 16
- 235000019341 magnesium sulphate Nutrition 0.000 claims description 16
- 230000010355 oscillation Effects 0.000 claims description 16
- 238000003756 stirring Methods 0.000 claims description 16
- 238000010792 warming Methods 0.000 claims description 16
- 230000003020 moisturizing effect Effects 0.000 claims description 15
- 238000009835 boiling Methods 0.000 claims description 10
- 238000011049 filling Methods 0.000 claims description 10
- 235000015165 citric acid Nutrition 0.000 claims description 9
- 239000000084 colloidal system Substances 0.000 claims description 9
- 238000009630 liquid culture Methods 0.000 claims description 9
- 238000000034 method Methods 0.000 claims description 9
- UXVMQQNJUSDDNG-UHFFFAOYSA-L Calcium chloride Chemical compound [Cl-].[Cl-].[Ca+2] UXVMQQNJUSDDNG-UHFFFAOYSA-L 0.000 claims description 8
- 244000068988 Glycine max Species 0.000 claims description 8
- 235000010469 Glycine max Nutrition 0.000 claims description 8
- 244000046052 Phaseolus vulgaris Species 0.000 claims description 8
- 235000010627 Phaseolus vulgaris Nutrition 0.000 claims description 8
- 235000006140 Raphanus sativus var sativus Nutrition 0.000 claims description 8
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 claims description 8
- 229910052921 ammonium sulfate Inorganic materials 0.000 claims description 8
- 235000011130 ammonium sulphate Nutrition 0.000 claims description 8
- 239000001110 calcium chloride Substances 0.000 claims description 8
- 229910001628 calcium chloride Inorganic materials 0.000 claims description 8
- 238000001816 cooling Methods 0.000 claims description 8
- 239000004744 fabric Substances 0.000 claims description 8
- 239000010440 gypsum Substances 0.000 claims description 8
- 229910052602 gypsum Inorganic materials 0.000 claims description 8
- 238000011081 inoculation Methods 0.000 claims description 8
- 238000004806 packaging method and process Methods 0.000 claims description 8
- 238000005057 refrigeration Methods 0.000 claims description 8
- 230000005070 ripening Effects 0.000 claims description 8
- 150000003839 salts Chemical class 0.000 claims description 8
- 239000008223 sterile water Substances 0.000 claims description 8
- 238000004659 sterilization and disinfection Methods 0.000 claims description 8
- 239000005720 sucrose Substances 0.000 claims description 8
- SMDQFHZIWNYSMR-UHFFFAOYSA-N sulfanylidenemagnesium Chemical compound S=[Mg] SMDQFHZIWNYSMR-UHFFFAOYSA-N 0.000 claims description 8
- 239000002253 acid Substances 0.000 claims description 7
- 229930003761 Vitamin B9 Natural products 0.000 claims description 2
- 230000009514 concussion Effects 0.000 claims description 2
- OVBPIULPVIDEAO-LBPRGKRZSA-N folic acid Chemical compound C=1N=C2NC(N)=NC(=O)C2=NC=1CNC1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 OVBPIULPVIDEAO-LBPRGKRZSA-N 0.000 claims description 2
- 235000000346 sugar Nutrition 0.000 claims description 2
- 235000019159 vitamin B9 Nutrition 0.000 claims description 2
- 239000011727 vitamin B9 Substances 0.000 claims description 2
- 235000005979 Citrus limon Nutrition 0.000 claims 2
- 244000131522 Citrus pyriformis Species 0.000 claims 2
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 claims 2
- 239000011591 potassium Substances 0.000 claims 2
- 229910052700 potassium Inorganic materials 0.000 claims 2
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 claims 1
- 241000382353 Pupa Species 0.000 claims 1
- 244000088415 Raphanus sativus Species 0.000 claims 1
- SEGLCEQVOFDUPX-UHFFFAOYSA-N di-(2-ethylhexyl)phosphoric acid Chemical compound CCCCC(CC)COP(O)(=O)OCC(CC)CCCC SEGLCEQVOFDUPX-UHFFFAOYSA-N 0.000 claims 1
- 229910052739 hydrogen Inorganic materials 0.000 claims 1
- 239000001257 hydrogen Substances 0.000 claims 1
- 125000004435 hydrogen atom Chemical class [H]* 0.000 claims 1
- 239000011574 phosphorus Substances 0.000 claims 1
- 229910052698 phosphorus Inorganic materials 0.000 claims 1
- 239000004576 sand Substances 0.000 claims 1
- 238000005303 weighing Methods 0.000 claims 1
- 235000013361 beverage Nutrition 0.000 abstract description 20
- 241000233866 Fungi Species 0.000 abstract description 6
- JVTAAEKCZFNVCJ-UHFFFAOYSA-N lactic acid Chemical compound CC(O)C(O)=O JVTAAEKCZFNVCJ-UHFFFAOYSA-N 0.000 abstract description 6
- 238000004519 manufacturing process Methods 0.000 abstract description 6
- 235000015097 nutrients Nutrition 0.000 abstract description 5
- 210000001124 body fluid Anatomy 0.000 abstract description 3
- 239000010839 body fluid Substances 0.000 abstract description 3
- 239000004310 lactic acid Substances 0.000 abstract description 3
- 235000014655 lactic acid Nutrition 0.000 abstract description 3
- 239000002994 raw material Substances 0.000 abstract description 3
- 241000190633 Cordyceps Species 0.000 abstract description 2
- 241000194017 Streptococcus Species 0.000 abstract description 2
- 235000010633 broth Nutrition 0.000 abstract description 2
- 235000013365 dairy product Nutrition 0.000 abstract description 2
- 235000020124 milk-based beverage Nutrition 0.000 abstract description 2
- 230000035790 physiological processes and functions Effects 0.000 abstract description 2
- 238000010791 quenching Methods 0.000 abstract description 2
- 239000003507 refrigerant Substances 0.000 abstract description 2
- 235000011497 sour milk drink Nutrition 0.000 abstract description 2
- 230000035922 thirst Effects 0.000 abstract description 2
- 241000186672 Lactobacillus delbrueckii subsp. bulgaricus Species 0.000 abstract 1
- 230000000694 effects Effects 0.000 description 16
- 230000006870 function Effects 0.000 description 12
- 239000011782 vitamin Substances 0.000 description 9
- 235000013343 vitamin Nutrition 0.000 description 9
- 229930003231 vitamin Natural products 0.000 description 9
- 229940088594 vitamin Drugs 0.000 description 9
- 238000012549 training Methods 0.000 description 8
- 150000003722 vitamin derivatives Chemical class 0.000 description 8
- 241000220259 Raphanus Species 0.000 description 7
- 239000000243 solution Substances 0.000 description 7
- 241000220324 Pyrus Species 0.000 description 6
- 239000000047 product Substances 0.000 description 6
- 235000020679 tap water Nutrition 0.000 description 6
- 230000036039 immunity Effects 0.000 description 5
- 235000014443 Pyrus communis Nutrition 0.000 description 4
- 230000000259 anti-tumor effect Effects 0.000 description 4
- 210000004369 blood Anatomy 0.000 description 4
- 239000008280 blood Substances 0.000 description 4
- 239000003814 drug Substances 0.000 description 4
- 230000002708 enhancing effect Effects 0.000 description 4
- 238000000265 homogenisation Methods 0.000 description 4
- 210000003734 kidney Anatomy 0.000 description 4
- 210000002540 macrophage Anatomy 0.000 description 4
- 238000012360 testing method Methods 0.000 description 4
- 206010011224 Cough Diseases 0.000 description 3
- 241000700605 Viruses Species 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 3
- 210000004027 cell Anatomy 0.000 description 3
- 230000007812 deficiency Effects 0.000 description 3
- 150000004676 glycans Chemical class 0.000 description 3
- 210000004072 lung Anatomy 0.000 description 3
- 238000002156 mixing Methods 0.000 description 3
- 239000002245 particle Substances 0.000 description 3
- 244000052769 pathogen Species 0.000 description 3
- 235000015206 pear juice Nutrition 0.000 description 3
- 238000012545 processing Methods 0.000 description 3
- 239000004475 Arginine Substances 0.000 description 2
- KQLDDLUWUFBQHP-UHFFFAOYSA-N Cordycepin Natural products C1=NC=2C(N)=NC=NC=2N1C1OCC(CO)C1O KQLDDLUWUFBQHP-UHFFFAOYSA-N 0.000 description 2
- 235000019750 Crude protein Nutrition 0.000 description 2
- FBPFZTCFMRRESA-KVTDHHQDSA-N D-Mannitol Chemical compound OC[C@@H](O)[C@@H](O)[C@H](O)[C@H](O)CO FBPFZTCFMRRESA-KVTDHHQDSA-N 0.000 description 2
- WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 description 2
- QNAYBMKLOCPYGJ-REOHCLBHSA-N L-alanine Chemical compound C[C@H](N)C(O)=O QNAYBMKLOCPYGJ-REOHCLBHSA-N 0.000 description 2
- ODKSFYDXXFIFQN-BYPYZUCNSA-P L-argininium(2+) Chemical compound NC(=[NH2+])NCCC[C@H]([NH3+])C(O)=O ODKSFYDXXFIFQN-BYPYZUCNSA-P 0.000 description 2
- COLNVLDHVKWLRT-QMMMGPOBSA-N L-phenylalanine Chemical compound OC(=O)[C@@H](N)CC1=CC=CC=C1 COLNVLDHVKWLRT-QMMMGPOBSA-N 0.000 description 2
- KZSNJWFQEVHDMF-BYPYZUCNSA-N L-valine Chemical compound CC(C)[C@H](N)C(O)=O KZSNJWFQEVHDMF-BYPYZUCNSA-N 0.000 description 2
- 229920001491 Lentinan Polymers 0.000 description 2
- 240000001307 Myosotis scorpioides Species 0.000 description 2
- KZSNJWFQEVHDMF-UHFFFAOYSA-N Valine Natural products CC(C)C(N)C(O)=O KZSNJWFQEVHDMF-UHFFFAOYSA-N 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 2
- 230000009471 action Effects 0.000 description 2
- 235000004279 alanine Nutrition 0.000 description 2
- 230000000844 anti-bacterial effect Effects 0.000 description 2
- 230000000840 anti-viral effect Effects 0.000 description 2
- ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 description 2
- 208000006673 asthma Diseases 0.000 description 2
- 230000001413 cellular effect Effects 0.000 description 2
- 238000005119 centrifugation Methods 0.000 description 2
- OFEZSBMBBKLLBJ-BAJZRUMYSA-N cordycepin Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](CO)C[C@H]1O OFEZSBMBBKLLBJ-BAJZRUMYSA-N 0.000 description 2
- OFEZSBMBBKLLBJ-UHFFFAOYSA-N cordycepine Natural products C1=NC=2C(N)=NC=NC=2N1C1OC(CO)CC1O OFEZSBMBBKLLBJ-UHFFFAOYSA-N 0.000 description 2
- 230000006378 damage Effects 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 239000003205 fragrance Substances 0.000 description 2
- 235000013922 glutamic acid Nutrition 0.000 description 2
- 239000004220 glutamic acid Substances 0.000 description 2
- 230000036541 health Effects 0.000 description 2
- 235000013402 health food Nutrition 0.000 description 2
- 210000001624 hip Anatomy 0.000 description 2
- 201000001881 impotence Diseases 0.000 description 2
- 230000000968 intestinal effect Effects 0.000 description 2
- 229940115286 lentinan Drugs 0.000 description 2
- 206010029410 night sweats Diseases 0.000 description 2
- 230000036565 night sweats Effects 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 230000035764 nutrition Effects 0.000 description 2
- 239000013618 particulate matter Substances 0.000 description 2
- 230000001717 pathogenic effect Effects 0.000 description 2
- 235000021017 pears Nutrition 0.000 description 2
- COLNVLDHVKWLRT-UHFFFAOYSA-N phenylalanine Natural products OC(=O)C(N)CC1=CC=CC=C1 COLNVLDHVKWLRT-UHFFFAOYSA-N 0.000 description 2
- 235000018102 proteins Nutrition 0.000 description 2
- 108090000623 proteins and genes Proteins 0.000 description 2
- 102000004169 proteins and genes Human genes 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 150000004671 saturated fatty acids Chemical class 0.000 description 2
- 230000002269 spontaneous effect Effects 0.000 description 2
- 238000005728 strengthening Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 208000024891 symptom Diseases 0.000 description 2
- 231100000331 toxic Toxicity 0.000 description 2
- 230000002588 toxic effect Effects 0.000 description 2
- 239000004474 valine Substances 0.000 description 2
- 239000011715 vitamin B12 Substances 0.000 description 2
- 239000011800 void material Substances 0.000 description 2
- 241000222485 Agaricales Species 0.000 description 1
- 241000221198 Basidiomycota Species 0.000 description 1
- 210000001239 CD8-positive, alpha-beta cytotoxic T lymphocyte Anatomy 0.000 description 1
- 244000223760 Cinnamomum zeylanicum Species 0.000 description 1
- 241001480006 Clavicipitaceae Species 0.000 description 1
- 229920000832 Cutin Polymers 0.000 description 1
- CMSMOCZEIVJLDB-UHFFFAOYSA-N Cyclophosphamide Chemical compound ClCCN(CCCl)P1(=O)NCCCO1 CMSMOCZEIVJLDB-UHFFFAOYSA-N 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 108060003951 Immunoglobulin Proteins 0.000 description 1
- WHUUTDBJXJRKMK-VKHMYHEASA-N L-glutamic acid Chemical compound OC(=O)[C@@H](N)CCC(O)=O WHUUTDBJXJRKMK-VKHMYHEASA-N 0.000 description 1
- 241000446313 Lamella Species 0.000 description 1
- 206010027476 Metastases Diseases 0.000 description 1
- 206010028980 Neoplasm Diseases 0.000 description 1
- 235000003283 Pachira macrocarpa Nutrition 0.000 description 1
- 244000131316 Panax pseudoginseng Species 0.000 description 1
- 235000005035 Panax pseudoginseng ssp. pseudoginseng Nutrition 0.000 description 1
- 235000003140 Panax quinquefolius Nutrition 0.000 description 1
- 241001529246 Platymiscium Species 0.000 description 1
- 241001492261 Pleurotaceae Species 0.000 description 1
- 244000292693 Poa annua Species 0.000 description 1
- 241000208000 Striga Species 0.000 description 1
- 210000001744 T-lymphocyte Anatomy 0.000 description 1
- 240000001085 Trapa natans Species 0.000 description 1
- 235000014364 Trapa natans Nutrition 0.000 description 1
- 229930003779 Vitamin B12 Natural products 0.000 description 1
- 240000006794 Volvariella volvacea Species 0.000 description 1
- 235000004501 Volvariella volvacea Nutrition 0.000 description 1
- 241001314279 Zoopagales Species 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 238000005054 agglomeration Methods 0.000 description 1
- 230000002776 aggregation Effects 0.000 description 1
- 230000002929 anti-fatigue Effects 0.000 description 1
- 230000002924 anti-infective effect Effects 0.000 description 1
- 230000002155 anti-virotic effect Effects 0.000 description 1
- 210000003056 antler Anatomy 0.000 description 1
- 235000009697 arginine Nutrition 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000004888 barrier function Effects 0.000 description 1
- 210000001185 bone marrow Anatomy 0.000 description 1
- 210000000481 breast Anatomy 0.000 description 1
- 239000001913 cellulose Substances 0.000 description 1
- 229920002678 cellulose Polymers 0.000 description 1
- 210000003169 central nervous system Anatomy 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 230000000973 chemotherapeutic effect Effects 0.000 description 1
- 238000002512 chemotherapy Methods 0.000 description 1
- 235000017803 cinnamon Nutrition 0.000 description 1
- AGVAZMGAQJOSFJ-WZHZPDAFSA-M cobalt(2+);[(2r,3s,4r,5s)-5-(5,6-dimethylbenzimidazol-1-yl)-4-hydroxy-2-(hydroxymethyl)oxolan-3-yl] [(2r)-1-[3-[(1r,2r,3r,4z,7s,9z,12s,13s,14z,17s,18s,19r)-2,13,18-tris(2-amino-2-oxoethyl)-7,12,17-tris(3-amino-3-oxopropyl)-3,5,8,8,13,15,18,19-octamethyl-2 Chemical compound [Co+2].N#[C-].[N-]([C@@H]1[C@H](CC(N)=O)[C@@]2(C)CCC(=O)NC[C@@H](C)OP(O)(=O)O[C@H]3[C@H]([C@H](O[C@@H]3CO)N3C4=CC(C)=C(C)C=C4N=C3)O)\C2=C(C)/C([C@H](C\2(C)C)CCC(N)=O)=N/C/2=C\C([C@H]([C@@]/2(CC(N)=O)C)CCC(N)=O)=N\C\2=C(C)/C2=N[C@]1(C)[C@@](C)(CC(N)=O)[C@@H]2CCC(N)=O AGVAZMGAQJOSFJ-WZHZPDAFSA-M 0.000 description 1
- 238000013329 compounding Methods 0.000 description 1
- 235000020247 cow milk Nutrition 0.000 description 1
- 239000008367 deionised water Substances 0.000 description 1
- 229910021641 deionized water Inorganic materials 0.000 description 1
- 230000001419 dependent effect Effects 0.000 description 1
- 230000004069 differentiation Effects 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 230000035622 drinking Effects 0.000 description 1
- 230000000857 drug effect Effects 0.000 description 1
- 235000015203 fruit juice Nutrition 0.000 description 1
- 235000012055 fruits and vegetables Nutrition 0.000 description 1
- 210000004051 gastric juice Anatomy 0.000 description 1
- 235000008434 ginseng Nutrition 0.000 description 1
- 239000003292 glue Substances 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 230000004217 heart function Effects 0.000 description 1
- 238000010438 heat treatment Methods 0.000 description 1
- 230000011132 hemopoiesis Effects 0.000 description 1
- 235000008216 herbs Nutrition 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 210000000987 immune system Anatomy 0.000 description 1
- 102000018358 immunoglobulin Human genes 0.000 description 1
- 239000002955 immunomodulating agent Substances 0.000 description 1
- 229940121354 immunomodulator Drugs 0.000 description 1
- 230000002584 immunomodulator Effects 0.000 description 1
- 230000007365 immunoregulation Effects 0.000 description 1
- 230000036512 infertility Effects 0.000 description 1
- 230000010354 integration Effects 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 210000000936 intestine Anatomy 0.000 description 1
- 230000003907 kidney function Effects 0.000 description 1
- 210000003127 knee Anatomy 0.000 description 1
- 210000000629 knee joint Anatomy 0.000 description 1
- 235000004213 low-fat Nutrition 0.000 description 1
- 238000005461 lubrication Methods 0.000 description 1
- 210000004698 lymphocyte Anatomy 0.000 description 1
- 230000035800 maturation Effects 0.000 description 1
- 239000002366 mineral element Substances 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 210000003205 muscle Anatomy 0.000 description 1
- 210000000822 natural killer cell Anatomy 0.000 description 1
- 239000002417 nutraceutical Substances 0.000 description 1
- 235000021436 nutraceutical agent Nutrition 0.000 description 1
- 230000009965 odorless effect Effects 0.000 description 1
- 231100000590 oncogenic Toxicity 0.000 description 1
- 230000002246 oncogenic effect Effects 0.000 description 1
- 230000001151 other effect Effects 0.000 description 1
- 244000045947 parasite Species 0.000 description 1
- 238000009928 pasteurization Methods 0.000 description 1
- 235000008729 phenylalanine Nutrition 0.000 description 1
- 239000000049 pigment Substances 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 239000002574 poison Substances 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 206010036596 premature ejaculation Diseases 0.000 description 1
- 238000003672 processing method Methods 0.000 description 1
- 238000001959 radiotherapy Methods 0.000 description 1
- 235000020185 raw untreated milk Nutrition 0.000 description 1
- 230000009257 reactivity Effects 0.000 description 1
- 108020003175 receptors Proteins 0.000 description 1
- 102000005962 receptors Human genes 0.000 description 1
- 238000011084 recovery Methods 0.000 description 1
- 230000002040 relaxant effect Effects 0.000 description 1
- 230000000241 respiratory effect Effects 0.000 description 1
- 235000009165 saligot Nutrition 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 230000036299 sexual function Effects 0.000 description 1
- 239000002002 slurry Substances 0.000 description 1
- 210000000952 spleen Anatomy 0.000 description 1
- 229910001220 stainless steel Inorganic materials 0.000 description 1
- 239000010935 stainless steel Substances 0.000 description 1
- 230000004936 stimulating effect Effects 0.000 description 1
- 239000002352 surface water Substances 0.000 description 1
- 235000019605 sweet taste sensations Nutrition 0.000 description 1
- 230000002195 synergetic effect Effects 0.000 description 1
- 210000002435 tendon Anatomy 0.000 description 1
- 238000002560 therapeutic procedure Methods 0.000 description 1
- 230000001256 tonic effect Effects 0.000 description 1
- 239000003053 toxin Substances 0.000 description 1
- 231100000765 toxin Toxicity 0.000 description 1
- 230000004614 tumor growth Effects 0.000 description 1
- 229910021642 ultra pure water Inorganic materials 0.000 description 1
- 239000012498 ultrapure water Substances 0.000 description 1
- 235000014393 valine Nutrition 0.000 description 1
- 210000001835 viscera Anatomy 0.000 description 1
- 235000019163 vitamin B12 Nutrition 0.000 description 1
- 230000037303 wrinkles Effects 0.000 description 1
- 235000013618 yogurt Nutrition 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23C—DAIRY PRODUCTS, e.g. MILK, BUTTER OR CHEESE; MILK OR CHEESE SUBSTITUTES; MAKING THEREOF
- A23C9/00—Milk preparations; Milk powder or milk powder preparations
- A23C9/152—Milk preparations; Milk powder or milk powder preparations containing additives
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/11—Lactobacillus
- A23V2400/123—Bulgaricus
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2400/00—Lactic or propionic acid bacteria
- A23V2400/21—Streptococcus, lactococcus
- A23V2400/249—Thermophilus
Abstract
The invention discloses a kind of preparation method of Cordyceps militaris mushroom functional form fermented beverage, mixed by extracting Cordyceps militaris zymotic fluid and Lentinus edodes fermented liquid with milk, add lactobacillus bulgaricus and streptococcus thermophilus ferments, composite sweetener and compound emulsion stabilizer are eventually adding, finally obtained Cordyceps militaris mushroom functional form fermented beverage.The Cordyceps militaris mushroom functional form fermented beverage of the present invention not only has the function that the refrigerant of different beverages and promotes the production of body fluid to quench thirst, also preferably save its nutrient, adjustable human body physiological function, the new edible fungus milk beverage, it is all to become the mode using dairy products as raw material through lactobacillus-fermented to have broken traditional sour milk beverage, and penetrate into streptococcus acidi lactici fermented solution among the zymotic fluid of cordyceps mycelia, shiitake mushroom hypha, become the new beverage of the elite and the function of lactic acid bacteria that integrate both mycelium fermentation broths.
Description
Technical field
The present invention relates to technical field of beverage processing, and in particular to a kind of preparation of Cordyceps militaris mushroom functional form fermented beverage
Method.
Background technology
Mushroom fruiting body is more slightly larger, bacteria cover diameter 5-12cm, up to 20cm, flattened spherical to slightly open and flat, surface water chestnut
Color, light brown, dark brown to deep cinnamon, there is dark scale, and often scale color is shallow to dirty white at edge, there is hair or wadding
Shape thing, bacterial context white is slightly thick or thick, fine and closely woven, lamella white, close, curved life, Length discrepancy.Given birth in stem to wilfully, white is often curved
Song, long 3-8cm, thick 0.5-1.5cm have fine striga below collarium, interior reality, cellulosic, and collarium easily disappears, white.Mushroom
Belong to Eumycota, Basidiomycetes, Agaricales, Pleurotaceae, mushroom platymiscium, also known as fragrant bacterium, flower mushroom on taxology, be commonly called as Chinese mushroom
Mushroom.Mushroom is the famous edible and medicinal fungi in China, and it is rare preferable health food to be referred to as " queen in mushroom " by people.It is fragrant
Mushroom category high protein, low fat, Hi CHO, high-cellulose nutraceutical, also containing abundant vitamin and mineral element.
Cordyceps militaris also known as Cordceps militaris, Cordyceps militaris are Ascomycotina, ergot Zoopagales, Clavicipitaceae, the type sepecies of Cordyceps,
Worldwide distribution natural resources quantity is seldom.Ancient medical book is recorded and modern research shows that northern Chinese caterpillar Fungus medical value and worm summer in winter
Grass is similar, has tonic effect and a variety of drug effects, can compare favourably with ginseng, pilose antler, is both a kind of rare edible mushroom, again
It is a kind of precious Chinese medicine.Cordyceps sinensis is the conventional Chinese medicine containing a variety of nutriments, and main component has：Cordycepic acid, crude protein
With glutamic acid, phenylalanine, valine, arginine, alanine and saturated fatty acid, unrighted acid and vitamin B12Deng thing
Matter.The function of cordyceps sinensis is enhancing body immunity, and qi-restoratives damages, strengthening the essence gas.Invigorating the lung and the kidney, controls kidney deficiency cough and asthma, spitting of blood, waist and knee acid
Bitterly, impotence and premature ejaculation, after being ill seminal emission, the asthenic symptoms such as long void, spontaneous perspiration, night sweat.
Progress and people's living standards continue to improve with social science and technology, people gradually start to pursue integration of drinking and medicinal herbs
Health food, for also increasingly paying attention to it as nutrient fruit and vegetable people standing in Cordyceps militaris and this kind of people of mushroom life
The exploitation of various new products, the wine of Cordyceps militaris bubble, beverage, yogurt, functional form blending milk are also subject to more and more consumers
Like.
Modulation breast is easy to the mouthfeel received, is occupied important one seat in the market with its abundant nutrition.How will
The all very strong these types of raw material of characteristic is ideally combined together, and is taken into account nutrition and health care, mouthfeel, stability several respects problem, is opened
Send the emphasis that the new varieties to meet the market requirement are always industry research.By retrieving Patents documents：Application No.
201310294107.7 disclosure of the invention a kind of processing method of Cordyceps militaris pear beverage, step are：The system of Cordyceps militaris extracting solution
It is standby：Take 2 parts~3 parts of fruiting bodies of cordyceps militaris to add certain deionized water, be boiled by fire, use slow boiling slightly boiling instead 20 minutes, it is cold
But to room temperature；The preparation of pears soak：1000 parts of ultra-pure water, adds 5 parts~20 parts vitamin Cs and 2 parts~10 parts pectin
Enzyme, is uniformly mixed；The preparation of pear juice clarified solution：150 parts~200 parts of pear, is added 2~5 times of pears soaks, is beaten using squeezing method
Slurry processing, pear juice clarified solution is obtained by filtration in 15 DEG C~20 DEG C standings；By Cordyceps militaris extracting solution and pear juice clarified solution with quality
Than being sufficiently mixed for 1: 1~1: 6, auxiliary material is added, mixes filtering, it is filling, it is vented, sealing, sterilizes, cool down at 100 DEG C.Have
Beneficial effect there is provided a kind of sour-sweet taste, the mixing fragrance with edible mushroom and pear, and can tonifying the lung to relieve cough, enhancing it is immune
The Cordyceps militaris pear beverage of power.
Cordyceps militaris is mostly used for making fruit juice or concoction beverage at present, does not have also and is combined it with cow's milk, and human body is mended
The fermentation for filling a large amount of vitamins and nutriment occurs.
The content of the invention
In view of this, in view of the deficiencies of the prior art, it is an object of the present invention to provide a kind of Cordyceps militaris mushroom functional form hair
The preparation method of ferment beverage.
In order to solve the above technical problems, the technical solution used in the present invention is：
A kind of preparation method of Cordyceps militaris mushroom functional form fermented beverage, includes the following steps：
S1, carry out Liquid Culture to Cordyceps militaris spawn, prepares Cordyceps militaris zymotic fluid, while carry out liquid training to mushroom strain
Support, prepare Lentinus edodes fermented liquid；
S2, will carry out being mixed to prepare mixed liquor after sterilization of milk with Cordyceps militaris zymotic fluid and Lentinus edodes fermented liquid, to mixed liquor
Defibrination is carried out, then with filtered through gauze, obtains filtrate；
S3, be warming up to filtrate 90~110 DEG C and keep 10~20min to sterilize, and then cooling is temporary；
In a small amount of filtrate to container in S4, taking-up step S3,116~127 DEG C of 20~40min of holding are again heated to
Sterilize, be subsequently cooled to 40~44 DEG C of inoculation domestications, the strain of access is lactobacillus bulgaricus and streptococcus thermophilus, is tamed and dociled
Strain after change expands culture as female bacterium leavening agent；
S5, take out in a small amount of filtrate to container in step S3, and addition sterile water dilutes 50~100 times, then by bar
Family name's bactericidal assay sterilizes postcooling to 22~28 DEG C, adds female bacterium leavening agent and final leavening is made；
S6, take out the filtrate in step S3 to fermentation tank, accesses obtained leavening in S5 steps and carries out fermentation system
Zymotic fluid is obtained, inoculum concentration is 4~8%, and temperature is 39~43 DEG C, and fermentation time is 4~12h；
Composite sweetener and compound emulsion stabilizer, be added in zymotic fluid by S7, heats up simultaneously high-pressure homogeneous, fermentation is made
Beverage；
S8, by fermented beverage is filling, packaging, be finally putting into freezer refrigeration and carry out after-ripening.
Further, the preparation method of the Cordyceps militaris zymotic fluid includes the following steps：
A, Cordyceps militaris activates：Slant medium is prepared first, and then slant medium is placed into autoclave and keeps temperature
116~127 DEG C of 20~40min of sterilizing of degree, slant medium is finally cooled down and Cordyceps militaris spawn is added to slant medium
In, it is put into temperature and is maintained at 5~9d of culture in 18~26 DEG C of constant incubator, the slant medium is by following parts by weight
Material is made：Potato 150~250 parts (liquors), 15~25 parts of glucose, KH2P041~2 part, 17~21 parts of beef extract,
15~25 parts of agar, pH are natural, 900~1100 parts of water；
B, liquid level-one expands culture：First cell culture medium is prepared first, and first cell culture medium is placed into autoclave and is kept
116~127 DEG C of 20~40min of sterilizing of temperature, by the strain access first cell culture medium after obtained activation in above-mentioned steps, put
Enter temperature and be maintained at 4~8d of shaking table culture in 18~26 DEG C of constant incubator, the first cell culture medium is by following parts by weight
Material is made：18~22 parts of peptone, 27~33 parts of glucose, 0.4~0.3 part of KH2P04, vitaminB10 .01~0.02
Part, 2~3 parts of salt, 900~1100 parts of water；
C, liquid two stage expands culture：Secondary medium is prepared first, and secondary medium is placed into autoclave and is kept
116~127 DEG C of 20~40min of sterilizing of temperature, secondary medium is accessed by the obtained strain expanded after cultivating in above-mentioned steps
Interior, inoculum concentration is 3~9%, is put into temperature and is maintained at 4~8d of shaking table culture in 18~26 DEG C of constant incubator, shaking table shakes
It is 80~120r/min to swing frequency, is made Cordyceps militaris zymotic fluid, the preparation of the two level culture be first put into 26~34 parts of base dregs of beans,
Each 4~6 parts of 30~33 parts of peptone, beef extract, ammonium sulfate, soya-bean cake, then plus boiling boil 10 minutes, then add 8~12 parts of white sugar,
0.9~1.1 part of potassium dihydrogen phosphate, 0.4~0.6 part of magnesium sulfate, 0.9~1.1 part of gypsum, 9~11 parts of peptone, moisturizing to 900
~1100 parts, stirring and dissolving.
Further, the preparation method of the Lentinus edodes fermented liquid includes the following steps：
A, mushroom strain activates：Prepare activation medium, by activation medium be placed into autoclave keep temperature 116~
127 DEG C sterilizing 15~25min, by mushroom strain access activation medium in, inoculum concentration be 8~12%, at 20~30 DEG C in
5~9d of culture in constant incubator, the concussion frequency of shaking table is 125~175r/min, and the preparation of the activation medium is first put
Enter 150~250 parts of potato, 1~3 part of sucrose, 1~3 part of peptone, 1~3 part of magnesium sulfate, 1~3 part of potassium dihydrogen phosphate, radish
130~170 parts, 18~22 parts of agar boil, filtered fluid is then obtained by filtration and adds water to be supplemented to 900~1100 parts, pH is natural；
B, mushroom strain culture：Glucose fermentation culture medium is prepared, glucose fermentation culture medium is placed into autoclave
116~127 DEG C of 15~25min of sterilizing of temperature are kept, the activation mushroom strain access glucose fermentation training that above-mentioned steps are obtained
Support in base, inoculum concentration is 8~12%, and it is 4.5~6.5 to ensure pH value, in shaking table culture 1 in constant incubator at 20~30 DEG C
~3d, the frequency of oscillation of shaking table is 125~175r/min, Lentinus edodes fermented liquid is made, the glucose fermentation culture medium is by as follows
The material of parts by weight is made：18~22 parts of glucose, 1~3 part of peptone, 0.4~0.6 part of sulphur magnesium, 0~12 part of vitamin B11,
0.4~0.6 part of potassium dihydrogen phosphate, 0.4~0.6 part of calcium chloride, stirring and dissolving, to 900~1100 parts, it is 5.5 to adjust pH for moisturizing.
Further, cordyceps sinensis fermentation liquor, Lentinus edodes fermented liquid, the mixed proportion of milk are 2~6 in step S2：2~6：6~
18。
Further, mixed liquor is by colloid mill defibrination in step S2, with 100 mesh filter-cloth filterings.
Further, the ratio of lactobacillus bulgaricus and streptococcus thermophilus is 1 in step S4:1.
Further, composite sweetener includes Semen Coicis syrup and white granulated sugar in step S7, and Semen Coicis syrup accounts for the matter of zymotic fluid
It is 0.15~0.5% to measure percentage, and the mass percent that white granulated sugar accounts for zymotic fluid is 1~2%.
Further, compound emulsion stabilizer includes pectin, citric acid and beta-cyclodextrin in step S7, and pectin accounts for fermentation
The mass percent of liquid is 2~4%, and the mass percent that citric acid accounts for zymotic fluid is 0.01~0.03%, and beta-cyclodextrin accounts for
The mass percent of zymotic fluid is 0.02~0.03%.
Further, zymotic fluid is warming up to homogeneous 1~6 time under 50~70 DEG C and 10~45MPa in step S7.
Further, ripening time is 12~24h in step S8.
Cordyceps militaris is the conventional Chinese medicine containing a variety of nutriments, and main component has：Cordycepic acid, crude protein and glutamic acid,
The material such as phenylalanine, valine, arginine, alanine and saturated fatty acid, unrighted acid and vitamin B12.Cordyceps sinensis
Function for enhancing body immunity, qi-restoratives damage, strengthening the essence gas.Invigorating the lung and the kidney, it is early to control kidney deficiency cough and asthma, spitting of blood, soreness of waist and knee joint, impotence
Let out, pass out semen, asthenic symptoms such as void, spontaneous perspiration, night sweat long after being ill.Cordyceps militaris not only has adjustment effect to the function of the various internal organs of human body,
Also there are some directly disease-resistant functions：Function of immune system, direct antitumor action are adjusted, cellular energy is improved, is antifatigue, adjusting
Cardiac function is saved, Respiratory ft tive resistance is adjusted, adjusts renal function, adjust hematopoiesis function, adjust blood fat, and cordyceps sinensis is also
With effects such as directly antiviral, adjusting central nervous system function, adjusting sexual functions.Exist in Cordyceps militaris zymotic fluid substantial amounts of
Cordycepin, have it is antiviral, antibacterial, substantially suppress tumour growth, have obvious synergistic effect with endoxan, and have drop blood
The effect of sugar, again with nourishing, health-care effect in addition to its is medicinal.Cordyceps sinensis polysaccharide colorless and odorless, water-soluble and stability is good, without poison
Side effect, can not only improve immunity of organisms, suppress tumour, and to the larger anti-tumor chemotherapeutic medicine of some toxic side effects
Thing has Synergy and attenuation effect.
Contain substantial amounts of lentinan in Lentinus edodes fermented liquid, be a kind of immunomodulator, can prevent caused by chemical factor
Oncogenic function, metastases of drawing up, enhancing body include different bacterium, parasite and virus the resistance of HIV, and it
Almost without any side effect, the after operation, radiotherapy, chemotherapy the 4th kind of biology of being known as is exempted from service therapy.It has four
Big effect：
Immunoregulation effect：By stimulating key cells (such as T lymphocytes, bone-marrow-derived lymphocyte, macrophage, NK cells
Deng) maturation, differentiation and propagation, improve host body balance, reach recovery or improve host cell to lymphokine, hormone and
The reactivity effect of other bioactie agents.
Antitumor action:By host strengthen induced activation macrophage and killer T cell, improve NK cytoactives and
Strengthen antibody dependent macrophage toxic action to play its antitumor action.
Antivirus action:Swash intravital macrophage, increase by mono- 2 expression of receptor of CD4+ cells and I L, induce I FN,
Improve NK cytoactives.
Anti-infectious function:The glycan molecule that dissociates in body fluid is combined with virus, plays shielding action, prevent pathogen with it is normal thin
Vigorous attached effect occurs for the glycan molecule of cellular surface.
The present invention uses lactobacillus bulgaricus and streptococcus thermophilus, and the smooth easily product for producing acquisition possesses curdled milk state
Smooth exquisiteness, production is sour soon, and production is glutinous and produces fragrance energy height and possesses weaker postacidification, deep to be favored by client, it, which has, presses down
The growth of pathogen processed, adjusts intestinal microenvironment；Acid resistance is stronger, and the enteron aisle that can be gone directly by gastric juice forms barrier, adjusts intestines
The effect of road strengthen immunity.Immunoglobulin is rich in milk, can efficiently resist virus, bacterium, fungi and other anaphylactogens,
Antibody is formed, neutralizes a toxin, builds up health, improves immunity, diseased probability is reduced, has good facilitation for human body,
Raw milk is used after checking and accepting and used, and examination meets national standard, GB 19301-2010.
The present invention provides a kind of preparation method of Cordyceps militaris mushroom functional form fermented beverage, is fermented by extracting Cordyceps militaris
Liquid, Lentinus edodes fermented liquid retain Cordyceps militaris and the mushroom element beneficial to human body, and can speed up the absorption of human body, then with milk
Mixing carries out fermentation and produces lactic acid bacteria, is conducive to human body intestinal canal function, carries out comprehensive adjustment to the body of person who quote, more
Beneficial to absorption of the human body to nutrient.
Cordyceps militaris zymotic fluid and Lentinus edodes fermented liquid are mixed as important nutrient with milk, but two kinds of zymotic fluids because
The reason for produce mode, liquid is interior to contain substantial amounts of suspended particulate substance in small, broken bits, it is therefore desirable to passes through colloid mill and carries out defibrination, glue
Body mill is coordinated by the opposite fixed tooth interlocked at a high speed with moving teeth, the particulate matter in liquid is pulverized, it integrally all uses food-grade
Stainless steel is made, pollution-free not corrode, and the mixed liquor after defibrination is filtered by 100 mesh filter clothes, by still remaining particulate matter
Filter out, ensure the final mouthfeel of beverage.
Filtrate after milk is filtered with the mixed liquor of Cordyceps militaris zymotic fluid and Lentinus edodes fermented liquid is gone out by the holding that heats up
Bacterium, ensures in filtrate without harmful levels of pathogens, and cordycepin and lentinan will not be then affected by it, it is ensured that the battalion in filtrate
Support element.Filtrate is first tamed strain before addition lactobacillus bulgaricus and streptococcus thermophilus, takes a small amount of filtrate
Strain is tamed, the filtrate of taking-up accounts for sum and is no more than 1%, and it is actual raw then to expand culture satisfaction to the strain after domestication
The demand of production.Take out a small amount of filtrate again in step s 5, the filtrate of taking-up accounts for sum and is no more than 1%, is diluted then
Obtained female bacterium leavening agent in step S4 is added, can survive by two kinds of fermenting microbes of pasteurization sterility assurance still can
To murder other harmful bacterias, final leavening to be used can be made after adding female bacterium leavening agent, carried out abundant dilution,
Easy to the subsequently fermentation to filtrate, the activity of leavening can be also fully ensured that；The pH value that PH is mixed according to it naturally, no
With being allocated again.
Strain is kept to ferment at a suitable temperature after filtrate access leavening, then by composite sweetener and compounding
Emulsion stabilizer is added thereto, and composite sweetener uses Semen Coicis syrup and white granulated sugar, and white granulated sugar can ensure sugariness and sweet taste
Mouthfeel, neutralize the taste of Cordyceps militaris zymotic fluid and Lentinus edodes fermented liquid, Semen Coicis syrup Semen Coicis has relaxing muscles and tendons eliminating impediment, invigorating spleen to remove dampness, disappears
Swollen apocenosis and other effects.Having makes skin smooth, and the effect of eliminating pigment spot, reduce wrinkle, the albumen matter contained in the middle decomposes ferment
The very rich thickness of element, can make the cutin softening of skin, have the function that to make skin smooth.Compound emulsion stabilizer using pectin,
Citric acid and beta-cyclodextrin, improve dispersiveness of the fat in mixture, make fatty particle fine, be evenly distributed, improve fermentation
The mouthfeel of beverage；Promote the interaction of fat and protein, make emulsion unstability or demulsification, contribute to fatty agglomeration and cohesion
Effect；Prevent that there are big particle in fermented beverage；The mouthfeel of fermented beverage exquisiteness is assigned, increases viscosity, taste lubrication.Finally
By heating and it is high-pressure homogeneous, further ensure fermented beverage fine and smooth mouthfeel, be not in big particle, further carry
The sophistication of high beverage.
The Cordyceps militaris mushroom functional form fermented beverage of the present invention not only has the refrigerant of different beverages and the work to promote the production of body fluid to quench thirst
With, also preferably save its nutrient, adjust human body physiological function, the new edible fungus milk beverage, has broken tradition
Sour milk beverage be all to become the mode using dairy products as raw material through lactobacillus-fermented, and streptococcus acidi lactici fermented solution is penetrated into cordyceps sinensis
Mycelium, shiitake mushroom hypha zymotic fluid among, become collect both mycelium fermentation broths elite and the function of lactic acid bacteria be
The new beverage of one.
Brief description of the drawings
Fig. 1 is the layering ratio of product and homogenization pressure relation schematic diagram in the present invention；
Fig. 2 is the layering ratio of product and homogenization cycles relation schematic diagram in the present invention.
Embodiment
For a better understanding of the present invention, present disclosure, but this hair are further fairly set out with reference to embodiment
Bright protection content is not limited solely to the following examples, and embodiment is as follows：
Cordyceps militaris spawn uses Cordyceps militaris agricultural university：Cm-029B, mushroom strain use mushroom 2.
Embodiment one：
A kind of preparation method of Cordyceps militaris mushroom functional form fermented beverage is present embodiments provided, is included the following steps：
S1, carry out Liquid Culture to Cordyceps militaris spawn, prepares Cordyceps militaris zymotic fluid, while carry out liquid training to mushroom strain
Support, prepare Lentinus edodes fermented liquid；
S2, will carry out being mixed to prepare mixed liquor after sterilization of milk with Cordyceps militaris zymotic fluid and Lentinus edodes fermented liquid, to mixed liquor
Defibrination is carried out, then with filtered through gauze, obtains filtrate；
S3, be warming up to filtrate 100 DEG C and keep 15min to sterilize, and then cooling is temporary；
In a small amount of filtrate to container in S4, taking-up step S3, it is again heated to 121 DEG C and keeps 30min to sterilize, so
Postcooling is to 42 DEG C of inoculation domestications, and the strain of access is lactobacillus bulgaricus and streptococcus thermophilus, and the strain after domestication expands
Culture is as female bacterium leavening agent；
In a small amount of filtrate to container in S5, taking-up step S3, addition sterile water dilutes 75 times, then passes through pasteurize
Method sterilizes postcooling to 25 DEG C, adds female bacterium leavening agent and final leavening is made；
S6, take out the filtrate in step S3 to fermentation tank, accesses obtained leavening in S5 steps and carries out fermentation system
Zymotic fluid, inoculum concentration 6% are obtained, temperature is 41 DEG C, fermentation time 8h；
Composite sweetener and compound emulsion stabilizer, be added in zymotic fluid by S7, heats up simultaneously high-pressure homogeneous, fermentation is made
Beverage；
S8, by fermented beverage is filling, packaging, be finally putting into freezer refrigeration and carry out after-ripening.
Further, the preparation method of the Cordyceps militaris zymotic fluid includes the following steps：
A, Cordyceps militaris activates：Slant medium is prepared first, and then slant medium is placed into autoclave and keeps temperature
121 DEG C of sterilizing 30min of degree, slant medium is finally cooled down and Cordyceps militaris spawn is added in slant medium, be put into temperature
Degree is maintained at culture 7d, the slant medium in 22 DEG C of constant incubator and is made of the material of following parts by weight：Potato
200 parts (liquors), 20 parts of glucose, KH2P041.5 parts, 19 parts of beef extract, 20 parts of agar, pH be natural, 1000 parts of water；
B, liquid level-one expands culture：First cell culture medium is prepared first, and first cell culture medium is placed into autoclave and is kept
121 DEG C of sterilizing 30min of temperature, by the strain access first cell culture medium after obtained activation in above-mentioned steps, are put into temperature guarantor
The shaking table culture 6d in 22 DEG C of constant incubator is held, the first cell culture medium is made of the material of following parts by weight：Peptone
20 parts, 30 parts of glucose, KH2P040.5 parts .01 parts of vitaminB10,2.5 parts of salt, 1000 parts of water；
C, liquid two stage expands culture：Secondary medium is prepared first, and secondary medium is placed into autoclave and is kept
121 DEG C of sterilizing 30min of temperature, the obtained strain expanded after cultivating in above-mentioned steps are accessed in secondary medium, inoculum concentration
For a1%, it is put into temperature and is maintained at shaking table culture a3d in a2 DEG C of constant incubator, the frequency of oscillation of shaking table is a4r/min, system
Cordyceps militaris zymotic fluid, the preparation of the two level culture be first put into 30 parts of base dregs of beans, 31.5 parts of peptone, beef extract, ammonium sulfate,
Each 5.00 parts of soya-bean cake, then plus 1000 parts of boiling tap waters 10 minutes, then add 10.00 parts of white sugar, 1.00 parts of potassium dihydrogen phosphate,
0.50 part of magnesium sulfate, 1.00 parts of gypsum, 10.00 parts of peptone, moisturizing are to 1000 parts, stirring and dissolving.
Further, the preparation method of the Lentinus edodes fermented liquid includes the following steps：
A, mushroom strain activates：Activation medium is prepared, activation medium is placed into 121 DEG C of temperature of holding in autoclave
Sterilize 20min, and mushroom strain is accessed in activation medium, and in cultivating 7d in constant incubator at 25 DEG C, the activation is trained
The preparation for supporting base is first put into 200 parts of potato, 2 parts of sucrose, 2 parts of peptone, 2 parts of magnesium sulfate, 2 parts of potassium dihydrogen phosphate, radish 150
Part, 20 parts of agar boil, and filtered fluid is then obtained by filtration and adds water to be supplemented to 1000 parts, pH is natural；
B, mushroom strain culture：Glucose fermentation culture medium is prepared, glucose fermentation culture medium is placed into autoclave
121 DEG C of sterilizing 20min of temperature are kept, in the activation mushroom strain access glucose fermentation culture medium that above-mentioned steps are obtained, are connect
Kind amount is 8%, and it is 5.5 to ensure pH value, is in shaking table culture 2d in constant incubator, the frequency of oscillation of shaking table at 25 DEG C
150r/min, is made Lentinus edodes fermented liquid, and the glucose fermentation culture medium is made of the material of following parts by weight：Glucose 20
Part, 2 parts of peptone, 0.5 part of sulphur magnesium, 1 part of vitamin B11,0.5 part of potassium dihydrogen phosphate, 0.5 part of calcium chloride, stirring and dissolving, are mended
For water to 1000 parts, it is 5.5 to adjust pH.
Further, cordyceps sinensis fermentation liquor, Lentinus edodes fermented liquid, the mixed proportion of milk are 4 in the step S2： 4：12.
Further, mixed liquor is by colloid mill defibrination in the step S2, with 100 mesh filter-cloth filterings.
Further, the ratio of lactobacillus bulgaricus and streptococcus thermophilus is 1 in the step S4:1.
Further, composite sweetener includes Semen Coicis syrup and white granulated sugar in the step S7, and Semen Coicis syrup accounts for zymotic fluid
Mass percent be 0.3%, white granulated sugar account for zymotic fluid mass percent be 1.5%.
Further, compound emulsion stabilizer includes pectin, citric acid and beta-cyclodextrin in the step S7, and pectin accounts for
The mass percent of zymotic fluid is 3%, and the mass percent that citric acid accounts for zymotic fluid is 0.02%, and beta-cyclodextrin accounts for zymotic fluid
Mass percent be 0.025%.
Further, zymotic fluid is warming up under 60 DEG C and 30MPa homogeneous three times in the step S7.
Further, ripening time is 18h in the step S8.
The embodiment studies the condition of culture of Cordyceps militaris zymotic fluid, three groups of experiments is carried out, with mycelium mycelia
The dry weight (g/100mL) of body is deliberated index, carries out orthogonal test.Shown in table specific as follows：
It is as follows to list orthogonal arrage：
Optimal technological condition for fermentation is inoculum concentration 5% as seen from the above table, and 22 DEG C, fermentation time 6d of fermentation temperature, vibrates
Frequency 100r/min.
Embodiment two：
A kind of preparation method of Cordyceps militaris mushroom functional form fermented beverage is present embodiments provided, is included the following steps：
S1, carry out Liquid Culture to Cordyceps militaris spawn, prepares Cordyceps militaris zymotic fluid, while carry out liquid training to mushroom strain
Support, prepare Lentinus edodes fermented liquid；
S2, will carry out being mixed to prepare mixed liquor after sterilization of milk with Cordyceps militaris zymotic fluid and Lentinus edodes fermented liquid, to mixed liquor
Defibrination is carried out, then with filtered through gauze, obtains filtrate；
S3, be warming up to filtrate 100 DEG C and keep 15min to sterilize, and then cooling is temporary；
In a small amount of filtrate to container in S4, taking-up step S3, it is again heated to 121 DEG C and keeps 30min to sterilize, so
Postcooling is to 42 DEG C of inoculation domestications, and the strain of access is lactobacillus bulgaricus and streptococcus thermophilus, and the strain after domestication expands
Culture is as female bacterium leavening agent；
In a small amount of filtrate to container in S5, taking-up step S3, addition sterile water dilutes 75 times, then passes through pasteurize
Method sterilizes postcooling to 25 DEG C, adds female bacterium leavening agent and final leavening is made；
S6, take out the filtrate in step S3 to fermentation tank, accesses obtained leavening in S5 steps and carries out fermentation system
Zymotic fluid, inoculum concentration 6% are obtained, temperature is 41 DEG C, fermentation time 8h；
Composite sweetener and compound emulsion stabilizer, be added in zymotic fluid by S7, heats up simultaneously high-pressure homogeneous, fermentation is made
Beverage；
S8, by fermented beverage is filling, packaging, be finally putting into freezer refrigeration and carry out after-ripening.
Further, the preparation method of the Cordyceps militaris zymotic fluid includes the following steps：
A, Cordyceps militaris activates：Slant medium is prepared first, and then slant medium is placed into autoclave and keeps temperature
121 DEG C of sterilizing 30min of degree, slant medium is finally cooled down and Cordyceps militaris spawn is added in slant medium, be put into temperature
Degree is maintained at culture 7d, the slant medium in 22 DEG C of constant incubator and is made of the material of following parts by weight：Potato
200 parts (liquors), 20 parts of glucose, KH2P041.5 parts, 19 parts of beef extract, 20 parts of agar, pH be natural, 1000 parts of water；
B, liquid level-one expands culture：First cell culture medium is prepared first, and first cell culture medium is placed into autoclave and is kept
121 DEG C of sterilizing 30min of temperature, by the strain access first cell culture medium after obtained activation in above-mentioned steps, are put into temperature guarantor
The shaking table culture 6d in 22 DEG C of constant incubator is held, the first cell culture medium is made of the material of following parts by weight：Peptone
20 parts, 30 parts of glucose, KH2P040.5 parts .01 parts of vitaminB10,2.5 parts of salt, 1000 parts of water；
C, liquid two stage expands culture：Secondary medium is prepared first, and secondary medium is placed into autoclave and is kept
121 DEG C of sterilizing 30min of temperature, the obtained strain expanded after cultivating in above-mentioned steps are accessed in secondary medium, inoculum concentration
For 5%, it is put into temperature and is maintained at shaking table culture 6d in 22 DEG C of constant incubator, the frequency of oscillation of shaking table is 100r/min, system
Cordyceps militaris zymotic fluid, the preparation of the two level culture be first put into 30 parts of base dregs of beans, 31.5 parts of peptone, beef extract, ammonium sulfate,
Each 5.00 parts of soya-bean cake, then plus 1000 parts of boiling tap waters 10 minutes, then add 10.00 parts of white sugar, 1.00 parts of potassium dihydrogen phosphate,
0.50 part of magnesium sulfate, 1.00 parts of gypsum, 10.00 parts of peptone, moisturizing are to 1000 parts, stirring and dissolving.
Further, the preparation method of the Lentinus edodes fermented liquid includes the following steps：
A, mushroom strain activates：Activation medium is prepared, activation medium is placed into 121 DEG C of temperature of holding in autoclave
Sterilize 20min, and mushroom strain is accessed in activation medium, and in cultivating 7d in constant incubator at 25 DEG C, the activation is trained
The preparation for supporting base is first put into 200 parts of potato, 2 parts of sucrose, 2 parts of peptone, 2 parts of magnesium sulfate, 2 parts of potassium dihydrogen phosphate, radish 150
Part, 20 parts of agar boil, and filtered fluid is then obtained by filtration and adds water to be supplemented to 1000 parts, pH is natural；
B, mushroom strain culture：Glucose fermentation culture medium is prepared, glucose fermentation culture medium is placed into autoclave
121 DEG C of sterilizing 20min of temperature are kept, in the activation mushroom strain access glucose fermentation culture medium that above-mentioned steps are obtained, are connect
Kind amount is b1%, ensures pH value b3, in shaking table culture b2d in constant incubator at 25 DEG C, the frequency of oscillation of shaking table is b4r/
Min, is made Lentinus edodes fermented liquid, and the glucose fermentation culture medium is made of the material of following parts by weight：20 parts of glucose, albumen
2 parts of peptone, 0.5 part of sulphur magnesium, 11 parts of vitamin B1,0.5 part of potassium dihydrogen phosphate, 0.5 part of calcium chloride, stirring and dissolving, moisturizing to 1000
Part, it is 5.5 to adjust pH.
Further, cordyceps sinensis fermentation liquor, Lentinus edodes fermented liquid, the mixed proportion of milk are 4 in the step S2： 4：12.
Further, mixed liquor is by colloid mill defibrination in the step S2, with 100 mesh filter-cloth filterings.
Further, the ratio of lactobacillus bulgaricus and streptococcus thermophilus is 1 in the step S4:1.
Further, composite sweetener includes Semen Coicis syrup and white granulated sugar in the step S7, and Semen Coicis syrup accounts for zymotic fluid
Mass percent be 0.3%, white granulated sugar account for zymotic fluid mass percent be 1.5%.
Further, compound emulsion stabilizer includes pectin, citric acid and beta-cyclodextrin in the step S7, and pectin accounts for
The mass percent of zymotic fluid is 3%, and the mass percent that citric acid accounts for zymotic fluid is 0.02%, and beta-cyclodextrin accounts for zymotic fluid
Mass percent be 0.025%.
Further, zymotic fluid is warming up under 60 DEG C and 30MPa homogeneous three times in the step S7.
Further, ripening time is 18h in the step S8.
The embodiment studies the condition of culture of Lentinus edodes fermented liquid, shown in table specific as follows：
Show that optimal conditions of fermentation is that inoculum concentration is 8% by orthogonal test, fermentation time 2d, frequency of oscillation 150r/
Min, pH 5.5.
Embodiment three：
A kind of preparation method of Cordyceps militaris mushroom functional form fermented beverage is present embodiments provided, is included the following steps：
S1, carry out Liquid Culture to Cordyceps militaris spawn, prepares Cordyceps militaris zymotic fluid, while carry out liquid training to mushroom strain
Support, prepare Lentinus edodes fermented liquid；
S2, will carry out being mixed to prepare mixed liquor after sterilization of milk with Cordyceps militaris zymotic fluid and Lentinus edodes fermented liquid, to mixed liquor
Defibrination is carried out, then with filtered through gauze, obtains filtrate；
S3, be warming up to filtrate 100 DEG C and keep 15min to sterilize, and then cooling is temporary；
In a small amount of filtrate to container in S4, taking-up step S3, it is again heated to 121 DEG C and keeps 30min to sterilize, so
Postcooling is to 42 DEG C of inoculation domestications, and the strain of access is lactobacillus bulgaricus and streptococcus thermophilus, and the strain after domestication expands
Culture is as female bacterium leavening agent；
In a small amount of filtrate to container in S5, taking-up step S3, addition sterile water dilutes 75 times, then passes through pasteurize
Method sterilizes postcooling to 25 DEG C, adds female bacterium leavening agent and final leavening is made；
S6, take out the filtrate in step S3 to fermentation tank, accesses obtained leavening in S5 steps and carries out fermentation system
Zymotic fluid, inoculum concentration 6% are obtained, temperature is 41 DEG C, fermentation time 8h；
Composite sweetener and compound emulsion stabilizer, be added in zymotic fluid by S7, heats up simultaneously high-pressure homogeneous, fermentation is made
Beverage；
S8, by fermented beverage is filling, packaging, be finally putting into freezer refrigeration and carry out after-ripening.
Further, the preparation method of the Cordyceps militaris zymotic fluid includes the following steps：
A, Cordyceps militaris activates：Slant medium is prepared first, and then slant medium is placed into autoclave and keeps temperature
121 DEG C of sterilizing 30min of degree, slant medium is finally cooled down and Cordyceps militaris spawn is added in slant medium, be put into temperature
Degree is maintained at culture 7d, the slant medium in 22 DEG C of constant incubator and is made of the material of following parts by weight：Potato
200 parts (liquors), 20 parts of glucose, KH2P041.5 parts, 19 parts of beef extract, 20 parts of agar, pH be natural, 1000 parts of water；
B, liquid level-one expands culture：First cell culture medium is prepared first, and first cell culture medium is placed into autoclave and is kept
121 DEG C of sterilizing 30min of temperature, by the strain access first cell culture medium after obtained activation in above-mentioned steps, are put into temperature guarantor
The shaking table culture 6d in 22 DEG C of constant incubator is held, the first cell culture medium is made of the material of following parts by weight：Peptone
20 parts, 30 parts of glucose, KH2P040.5 parts .01 parts of vitaminB10,2.5 parts of salt, 1000 parts of water；
C, liquid two stage expands culture：Secondary medium is prepared first, and secondary medium is placed into autoclave and is kept
121 DEG C of sterilizing 30min of temperature, the obtained strain expanded after cultivating in above-mentioned steps are accessed in secondary medium, inoculum concentration
For 5%, it is put into temperature and is maintained at shaking table culture 6d in 22 DEG C of constant incubator, the frequency of oscillation of shaking table is 100r/min, system
Cordyceps militaris zymotic fluid, the preparation of the two level culture be first put into 30 parts of base dregs of beans, 31.5 parts of peptone, beef extract, ammonium sulfate,
Each 5.00 parts of soya-bean cake, then plus 1000 parts of boiling tap waters 10 minutes, then add 10.00 parts of white sugar, 1.00 parts of potassium dihydrogen phosphate,
0.50 part of magnesium sulfate, 1.00 parts of gypsum, 10.00 parts of peptone, moisturizing are to 1000 parts, stirring and dissolving.
Further, the preparation method of the Lentinus edodes fermented liquid includes the following steps：
A, mushroom strain activates：Activation medium is prepared, activation medium is placed into 121 DEG C of temperature of holding in autoclave
Sterilize 20min, and mushroom strain is accessed in activation medium, and in cultivating 7d in constant incubator at 25 DEG C, the activation is trained
The preparation for supporting base is first put into 200 parts of potato, 2 parts of sucrose, 2 parts of peptone, 2 parts of magnesium sulfate, 2 parts of potassium dihydrogen phosphate, radish 150
Part, 20 parts of agar boil, and filtered fluid is then obtained by filtration and adds water to be supplemented to 1000 parts, pH is natural；
B, mushroom strain culture：Glucose fermentation culture medium is prepared, glucose fermentation culture medium is placed into autoclave
121 DEG C of sterilizing 20min of temperature are kept, in the activation mushroom strain access glucose fermentation culture medium that above-mentioned steps are obtained, are connect
Kind amount is 8%, ensures pH value 5.5, in shaking table culture 2d in constant incubator at 25 DEG C, the frequency of oscillation of shaking table is 150r/
Min, is made Lentinus edodes fermented liquid, and the glucose fermentation culture medium is made of the material of following parts by weight：20 parts of glucose, albumen
2 parts of peptone, 0.5 part of sulphur magnesium, 11 parts of vitamin B1,0.5 part of potassium dihydrogen phosphate, 0.5 part of calcium chloride, stirring and dissolving, moisturizing to 1000
Part, it is 5.5 to adjust pH.
Further, cordyceps sinensis fermentation liquor, Lentinus edodes fermented liquid, the mixed proportion of milk are 4 in the step S2： 4：c.
Further, mixed liquor is by colloid mill defibrination in the step S2, with 100 mesh filter-cloth filterings.
Further, the ratio of lactobacillus bulgaricus and streptococcus thermophilus is 1 in the step S4:1.
Further, composite sweetener includes Semen Coicis syrup and white granulated sugar in the step S7, and Semen Coicis syrup accounts for zymotic fluid
Mass percent be 0.3%, white granulated sugar account for zymotic fluid mass percent be 1.5%.
Further, compound emulsion stabilizer includes pectin, citric acid and beta-cyclodextrin in the step S7, and pectin accounts for
The mass percent of zymotic fluid is 3%, and the mass percent that citric acid accounts for zymotic fluid is 0.02%, and beta-cyclodextrin accounts for zymotic fluid
Mass percent be 0.025%.
Further, zymotic fluid is warming up under 60 DEG C and 30MPa homogeneous three times in the step S7.
Further, ripening time is 18h in the step S8.
The embodiment tests the mixed proportion of cordyceps sinensis fermentation liquor, Lentinus edodes fermented liquid, milk, ensures Chinese caterpillar fungus fermentation
Liquid, mushroom ferment liquid proportional are 1：1, the ratio of milk is changed：
By upper table we can see that the optimal cordyceps sinensis fermentation liquor of domestication, Lentinus edodes fermented liquid, the mixed proportion of milk are
4：4：12.
Example IV：
A kind of preparation method of Cordyceps militaris mushroom functional form fermented beverage is present embodiments provided, is included the following steps：
S1, carry out Liquid Culture to Cordyceps militaris spawn, prepares Cordyceps militaris zymotic fluid, while carry out liquid training to mushroom strain
Support, prepare Lentinus edodes fermented liquid；
S2, will carry out being mixed to prepare mixed liquor after sterilization of milk with Cordyceps militaris zymotic fluid and Lentinus edodes fermented liquid, to mixed liquor
Defibrination is carried out, then with filtered through gauze, obtains filtrate；
S3, be warming up to filtrate 100 DEG C and keep 15min to sterilize, and then cooling is temporary；
In a small amount of filtrate to container in S4, taking-up step S3, it is again heated to 121 DEG C and keeps 30min to sterilize, so
Postcooling is to 42 DEG C of inoculation domestications, and the strain of access is lactobacillus bulgaricus and streptococcus thermophilus, and the strain after domestication expands
Culture is as female bacterium leavening agent；
In a small amount of filtrate to container in S5, taking-up step S3, addition sterile water dilutes 75 times, then passes through pasteurize
Method sterilizes postcooling to 25 DEG C, adds female bacterium leavening agent and final leavening is made；
S6, take out the filtrate in step S3 to fermentation tank, accesses obtained leavening in S5 steps and carries out fermentation system
Zymotic fluid, inoculum concentration d1% are obtained, temperature is d2 DEG C, fermentation time d3h；
Composite sweetener and compound emulsion stabilizer, be added in zymotic fluid by S7, heats up simultaneously high-pressure homogeneous, fermentation is made
Beverage；
S8, by fermented beverage is filling, packaging, be finally putting into freezer refrigeration and carry out after-ripening.
Further, the preparation method of the Cordyceps militaris zymotic fluid includes the following steps：
A, Cordyceps militaris activates：Slant medium is prepared first, and then slant medium is placed into autoclave and keeps temperature
121 DEG C of sterilizing 30min of degree, slant medium is finally cooled down and Cordyceps militaris spawn is added in slant medium, be put into temperature
Degree is maintained at culture 7d, the slant medium in 22 DEG C of constant incubator and is made of the material of following parts by weight：Potato
200 parts (liquors), 20 parts of glucose, KH2P041.5 parts, 19 parts of beef extract, 20 parts of agar, pH be natural, 1000 parts of water；
B, liquid level-one expands culture：First cell culture medium is prepared first, and first cell culture medium is placed into autoclave and is kept
121 DEG C of sterilizing 30min of temperature, by the strain access first cell culture medium after obtained activation in above-mentioned steps, are put into temperature guarantor
The shaking table culture 6d in 22 DEG C of constant incubator is held, the first cell culture medium is made of the material of following parts by weight：Peptone
20 parts, 30 parts of glucose, KH2P040.5 parts .01 parts of vitaminB10,2.5 parts of salt, 1000 parts of water；
C, liquid two stage expands culture：Secondary medium is prepared first, and secondary medium is placed into autoclave and is kept
121 DEG C of sterilizing 30min of temperature, the obtained strain expanded after cultivating in above-mentioned steps are accessed in secondary medium, inoculum concentration
For 5%, it is put into temperature and is maintained at shaking table culture 6d in 22 DEG C of constant incubator, the frequency of oscillation of shaking table is 100r/min, system
Cordyceps militaris zymotic fluid, the preparation of the two level culture be first put into 30 parts of base dregs of beans, 31.5 parts of peptone, beef extract, ammonium sulfate,
Each 5.00 parts of soya-bean cake, then plus 1000 parts of boiling tap waters 10 minutes, then add 10.00 parts of white sugar, 1.00 parts of potassium dihydrogen phosphate,
0.50 part of magnesium sulfate, 1.00 parts of gypsum, 10.00 parts of peptone, moisturizing are to 1000 parts, stirring and dissolving.
Further, the preparation method of the Lentinus edodes fermented liquid includes the following steps：
A, mushroom strain activates：Activation medium is prepared, activation medium is placed into 121 DEG C of temperature of holding in autoclave
Sterilize 20min, and mushroom strain is accessed in activation medium, and in cultivating 7d in constant incubator at 25 DEG C, the activation is trained
The preparation for supporting base is first put into 200 parts of potato, 2 parts of sucrose, 2 parts of peptone, 2 parts of magnesium sulfate, 2 parts of potassium dihydrogen phosphate, radish 150
Part, 20 parts of agar boil, and filtered fluid is then obtained by filtration and adds water to be supplemented to 1000 parts, pH is natural；
B, mushroom strain culture：Glucose fermentation culture medium is prepared, glucose fermentation culture medium is placed into autoclave
121 DEG C of sterilizing 20min of temperature are kept, in the activation mushroom strain access glucose fermentation culture medium that above-mentioned steps are obtained, are connect
Kind amount is 8%, ensures pH value 5.5, in shaking table culture 2d in constant incubator at 25 DEG C, the frequency of oscillation of shaking table is 150r/
Min, is made Lentinus edodes fermented liquid, and the glucose fermentation culture medium is made of the material of following parts by weight：20 parts of glucose, albumen
2 parts of peptone, 0.5 part of sulphur magnesium, 11 parts of vitamin B1,0.5 part of potassium dihydrogen phosphate, 0.5 part of calcium chloride, stirring and dissolving, moisturizing to 1000
Part, it is 5.5 to adjust pH.
Further, cordyceps sinensis fermentation liquor, Lentinus edodes fermented liquid, the mixed proportion of milk are 4 in the step S2： 4：12.
Further, mixed liquor is by colloid mill defibrination in the step S2, with 100 mesh filter-cloth filterings.
Further, the ratio of lactobacillus bulgaricus and streptococcus thermophilus is 1 in the step S4:1.
Further, composite sweetener includes Semen Coicis syrup and white granulated sugar in the step S7, and Semen Coicis syrup accounts for zymotic fluid
Mass percent be 0.3%, white granulated sugar account for zymotic fluid mass percent be 1.5%.
Further, compound emulsion stabilizer includes pectin, citric acid and beta-cyclodextrin in the step S7, and pectin accounts for
The mass percent of zymotic fluid is 3%, and the mass percent that citric acid accounts for zymotic fluid is 0.02%, and beta-cyclodextrin accounts for zymotic fluid
Mass percent be 0.025%.
Further, zymotic fluid is warming up under 60 DEG C and 30MPa homogeneous three times in the step S7.
Further, ripening time is 18h in the step S8.
The embodiment studies the fermentation condition of final mixed liquor, and specific form is as follows：
Production acid amount after being fermented with zymotic fluid is index, and optimal fermentation condition is can obtain as fermentation by orthogonal test table
41 DEG C, fermentation time 8h of temperature, inoculum concentration 6%.
Embodiment five：
A kind of preparation method of Cordyceps militaris mushroom functional form fermented beverage is present embodiments provided, is included the following steps：
S1, carry out Liquid Culture to Cordyceps militaris spawn, prepares Cordyceps militaris zymotic fluid, while carry out liquid training to mushroom strain
Support, prepare Lentinus edodes fermented liquid；
S2, will carry out being mixed to prepare mixed liquor after sterilization of milk with Cordyceps militaris zymotic fluid and Lentinus edodes fermented liquid, to mixed liquor
Defibrination is carried out, then with filtered through gauze, obtains filtrate；
S3, be warming up to filtrate 100 DEG C and keep 15min to sterilize, and then cooling is temporary；
In a small amount of filtrate to container in S4, taking-up step S3, it is again heated to 121 DEG C and keeps 30min to sterilize, so
Postcooling is to 42 DEG C of inoculation domestications, and the strain of access is lactobacillus bulgaricus and streptococcus thermophilus, and the strain after domestication expands
Culture is as female bacterium leavening agent；
In a small amount of filtrate to container in S5, taking-up step S3, addition sterile water dilutes 75 times, then passes through pasteurize
Method sterilizes postcooling to 25 DEG C, adds female bacterium leavening agent and final leavening is made；
S6, take out the filtrate in step S3 to fermentation tank, accesses obtained leavening in S5 steps and carries out fermentation system
Zymotic fluid, inoculum concentration 6% are obtained, temperature is 41 DEG C, fermentation time 8h；
Composite sweetener and compound emulsion stabilizer, be added in zymotic fluid by S7, heats up simultaneously high-pressure homogeneous, fermentation is made
Beverage；
S8, by fermented beverage is filling, packaging, be finally putting into freezer refrigeration and carry out after-ripening.
Further, the preparation method of the Cordyceps militaris zymotic fluid includes the following steps：
A, Cordyceps militaris activates：Slant medium is prepared first, and then slant medium is placed into autoclave and keeps temperature
121 DEG C of sterilizing 30min of degree, slant medium is finally cooled down and Cordyceps militaris spawn is added in slant medium, be put into temperature
Degree is maintained at culture 7d, the slant medium in 22 DEG C of constant incubator and is made of the material of following parts by weight：Potato
200 parts (liquors), 20 parts of glucose, KH2P041.5 parts, 19 parts of beef extract, 20 parts of agar, pH be natural, 1000 parts of water；
B, liquid level-one expands culture：First cell culture medium is prepared first, and first cell culture medium is placed into autoclave and is kept
121 DEG C of sterilizing 30min of temperature, by the strain access first cell culture medium after obtained activation in above-mentioned steps, are put into temperature guarantor
The shaking table culture 6d in 22 DEG C of constant incubator is held, the first cell culture medium is made of the material of following parts by weight：Peptone
20 parts, 30 parts of glucose, KH2P040.5 parts .01 parts of vitaminB10,2.5 parts of salt, 1000 parts of water；
C, liquid two stage expands culture：Secondary medium is prepared first, and secondary medium is placed into autoclave and is kept
121 DEG C of sterilizing 30min of temperature, the obtained strain expanded after cultivating in above-mentioned steps are accessed in secondary medium, inoculum concentration
For 5%, it is put into temperature and is maintained at shaking table culture 6d in 22 DEG C of constant incubator, the frequency of oscillation of shaking table is 100r/min, system
Cordyceps militaris zymotic fluid, the preparation of the two level culture be first put into 30 parts of base dregs of beans, 31.5 parts of peptone, beef extract, ammonium sulfate,
Each 5.00 parts of soya-bean cake, then plus 1000 parts of boiling tap waters 10 minutes, then add 10.00 parts of white sugar, 1.00 parts of potassium dihydrogen phosphate,
0.50 part of magnesium sulfate, 1.00 parts of gypsum, 10.00 parts of peptone, moisturizing are to 1000 parts, stirring and dissolving.
Further, the preparation method of the Lentinus edodes fermented liquid includes the following steps：
A, mushroom strain activates：Activation medium is prepared, activation medium is placed into 121 DEG C of temperature of holding in autoclave
Sterilize 20min, and mushroom strain is accessed in activation medium, and in cultivating 7d in constant incubator at 25 DEG C, the activation is trained
The preparation for supporting base is first put into 200 parts of potato, 2 parts of sucrose, 2 parts of peptone, 2 parts of magnesium sulfate, 2 parts of potassium dihydrogen phosphate, radish 150
Part, 20 parts of agar boil, and filtered fluid is then obtained by filtration and adds water to be supplemented to 1000 parts, pH is natural；
B, mushroom strain culture：Glucose fermentation culture medium is prepared, glucose fermentation culture medium is placed into autoclave
121 DEG C of sterilizing 20min of temperature are kept, in the activation mushroom strain access glucose fermentation culture medium that above-mentioned steps are obtained, are connect
Kind amount is 8%, ensures pH value 5.5, in shaking table culture 2d in constant incubator at 25 DEG C, the frequency of oscillation of shaking table is 150r/
Min, is made Lentinus edodes fermented liquid, and the glucose fermentation culture medium is made of the material of following parts by weight：20 parts of glucose, albumen
2 parts of peptone, 0.5 part of sulphur magnesium, 11 parts of vitamin B1,0.5 part of potassium dihydrogen phosphate, 0.5 part of calcium chloride, stirring and dissolving, moisturizing to 1000
Part, it is 5.5 to adjust pH.
Further, cordyceps sinensis fermentation liquor, Lentinus edodes fermented liquid, the mixed proportion of milk are 4 in the step S2： 4：12.
Further, mixed liquor is by colloid mill defibrination in the step S2, with 100 mesh filter-cloth filterings.
Further, the ratio of lactobacillus bulgaricus and streptococcus thermophilus is 1 in the step S4:1.
Further, composite sweetener includes Semen Coicis syrup and white granulated sugar in the step S7, and Semen Coicis syrup accounts for zymotic fluid
Mass percent be 0.3%, white granulated sugar account for zymotic fluid mass percent be 1.5%.
Further, compound emulsion stabilizer includes pectin, citric acid and beta-cyclodextrin in the step S7, and pectin accounts for
The mass percent of zymotic fluid is 3%, and the mass percent that citric acid accounts for zymotic fluid is 0.02%, and beta-cyclodextrin accounts for zymotic fluid
Mass percent be 0.025%.
Further, zymotic fluid is warming up to homogeneous f times under 60 DEG C and eMPa in the step S7.
Further, ripening time is 18h in the step S8.
The embodiment studies final fermented beverage processing condition, and homogeneous remains unchanged three times first, to homogeneous
Pressure be changed, it is group data of 3, e 10,15,20,25,30,35,40,45 8 to take f, centrifugation measure product layering
Rate is as shown in Figure 1, the pressure for learning homogeneous by Fig. 1 is preferably kept in 30MPa.
Then the number of homogeneous is studied, guarantee homogenization pressure is 30MPa, and homogenization cycles 1,2,3,4,5,6 are public
Six groups of data, measure the centrifugation layering ratio of product after different number homogeneous as shown in Fig. 2, learning that the number of homogeneous is preferred by Fig. 2
For 3 times.
Embodiment six：
A kind of preparation method of Cordyceps militaris mushroom functional form fermented beverage is present embodiments provided, is included the following steps：
S1, carry out Liquid Culture to Cordyceps militaris spawn, prepares Cordyceps militaris zymotic fluid, while carry out liquid training to mushroom strain
Support, prepare Lentinus edodes fermented liquid；
S2, will carry out being mixed to prepare mixed liquor after sterilization of milk with Cordyceps militaris zymotic fluid and Lentinus edodes fermented liquid, to mixed liquor
Defibrination is carried out, then with filtered through gauze, obtains filtrate；
S3, be warming up to filtrate 100 DEG C and keep 15min to sterilize, and then cooling is temporary；
In a small amount of filtrate to container in S4, taking-up step S3, it is again heated to 121 DEG C and keeps 30min to sterilize, so
Postcooling is to 42 DEG C of inoculation domestications, and the strain of access is lactobacillus bulgaricus and streptococcus thermophilus, and the strain after domestication expands
Culture is as female bacterium leavening agent；
In a small amount of filtrate to container in S5, taking-up step S3, addition sterile water dilutes 75 times, then passes through pasteurize
Method sterilizes postcooling to 25 DEG C, adds female bacterium leavening agent and final leavening is made；
S6, take out the filtrate in step S3 to fermentation tank, accesses obtained leavening in S5 steps and carries out fermentation system
Zymotic fluid, inoculum concentration 6% are obtained, temperature is 41 DEG C, fermentation time 8h；
Composite sweetener and compound emulsion stabilizer, be added in zymotic fluid by S7, heats up simultaneously high-pressure homogeneous, fermentation is made
Beverage；
S8, by fermented beverage is filling, packaging, be finally putting into freezer refrigeration and carry out after-ripening.
Further, the preparation method of the Cordyceps militaris zymotic fluid includes the following steps：
A, Cordyceps militaris activates：Slant medium is prepared first, and then slant medium is placed into autoclave and keeps temperature
121 DEG C of sterilizing 30min of degree, slant medium is finally cooled down and Cordyceps militaris spawn is added in slant medium, be put into temperature
Degree is maintained at culture 7d, the slant medium in 22 DEG C of constant incubator and is made of the material of following parts by weight：Potato
200 parts (liquors), 20 parts of glucose, KH2P041.5 parts, 19 parts of beef extract, 20 parts of agar, pH be natural, 1000 parts of water；
B, liquid level-one expands culture：First cell culture medium is prepared first, and first cell culture medium is placed into autoclave and is kept
121 DEG C of sterilizing 30min of temperature, by the strain access first cell culture medium after obtained activation in above-mentioned steps, are put into temperature guarantor
The shaking table culture 6d in 22 DEG C of constant incubator is held, the first cell culture medium is made of the material of following parts by weight：Peptone
20 parts, 30 parts of glucose, KH2P040.5 parts .01 parts of vitaminB10,2.5 parts of salt, 1000 parts of water；
C, liquid two stage expands culture：Secondary medium is prepared first, and secondary medium is placed into autoclave and is kept
121 DEG C of sterilizing 30min of temperature, the obtained strain expanded after cultivating in above-mentioned steps are accessed in secondary medium, inoculum concentration
For 5%, it is put into temperature and is maintained at shaking table culture 6d in 22 DEG C of constant incubator, the frequency of oscillation of shaking table is 100r/min, system
Cordyceps militaris zymotic fluid, the preparation of the two level culture be first put into 30 parts of base dregs of beans, 31.5 parts of peptone, beef extract, ammonium sulfate,
Each 5.00 parts of soya-bean cake, then plus 1000 parts of boiling tap waters 10 minutes, then add 10.00 parts of white sugar, 1.00 parts of potassium dihydrogen phosphate,
0.50 part of magnesium sulfate, 1.00 parts of gypsum, 10.00 parts of peptone, moisturizing are to 1000 parts, stirring and dissolving.
Further, the preparation method of the Lentinus edodes fermented liquid includes the following steps：
A, mushroom strain activates：Activation medium is prepared, activation medium is placed into 121 DEG C of temperature of holding in autoclave
Sterilize 20min, and mushroom strain is accessed in activation medium, and in cultivating 7d in constant incubator at 25 DEG C, the activation is trained
The preparation for supporting base is first put into 200 parts of potato, 2 parts of sucrose, 2 parts of peptone, 2 parts of magnesium sulfate, 2 parts of potassium dihydrogen phosphate, radish 150
Part, 20 parts of agar boil, and filtered fluid is then obtained by filtration and adds water to be supplemented to 1000 parts, pH is natural；
B, mushroom strain culture：Glucose fermentation culture medium is prepared, glucose fermentation culture medium is placed into autoclave
121 DEG C of sterilizing 20min of temperature are kept, in the activation mushroom strain access glucose fermentation culture medium that above-mentioned steps are obtained, are connect
Kind amount is 8%, ensures pH value 5.5, in shaking table culture 2d in constant incubator at 25 DEG C, the frequency of oscillation of shaking table is 150r/
Min, is made Lentinus edodes fermented liquid, and the glucose fermentation culture medium is made of the material of following parts by weight：20 parts of glucose, albumen
2 parts of peptone, 0.5 part of sulphur magnesium, 11 parts of vitamin B1,0.5 part of potassium dihydrogen phosphate, 0.5 part of calcium chloride, stirring and dissolving, moisturizing to 1000
Part, it is 5.5 to adjust pH.
Further, cordyceps sinensis fermentation liquor, Lentinus edodes fermented liquid, the mixed proportion of milk are 4 in the step S2： 4：12.
Further, mixed liquor is by colloid mill defibrination in the step S2, with 100 mesh filter-cloth filterings.
Further, the ratio of lactobacillus bulgaricus and streptococcus thermophilus is 1 in the step S4:1.
Further, composite sweetener includes Semen Coicis syrup and white granulated sugar in the step S7, and Semen Coicis syrup accounts for zymotic fluid
Mass percent be h%, the mass percent that white granulated sugar accounts for zymotic fluid is i%.
Further, compound emulsion stabilizer includes pectin, citric acid and beta-cyclodextrin in the step S7, and pectin accounts for
The mass percent of zymotic fluid is j%, and the mass percent that citric acid accounts for zymotic fluid is k%, and beta-cyclodextrin accounts for the matter of zymotic fluid
Amount percentage is m%.
Further, zymotic fluid is warming up to homogeneous 3 times under 60 DEG C and 30MPa in the step S7.
Further, ripening time is 18h in the step S8.
The embodiment studies the additive amount of composite sweetener and compound emulsion stabilizer in final fermented beverage,
Three groups of experiments are carried out, first group of h is 0.2, i 1.2, j 2.5, k 0.015, m 0.023；Second group of h is that 0.3, i is
1.5, j 3, k 0.02, m 0.025, the 3rd group of h is 0.4, i 1.8, and j 35, k 0.025, m 0.028, is felt
Official's scoring show that form is as follows：
Best of breed is that the mass percent that Semen Coicis syrup accounts for zymotic fluid is 3%, and white granulated sugar accounts for the quality percentage of zymotic fluid
It is 3% than the mass percent that for 1.5%, pectin accounts for zymotic fluid, the mass percent that citric acid accounts for zymotic fluid is 0.02%,
The mass percent that beta-cyclodextrin accounts for zymotic fluid is 0.025%.
Claims (10)
- A kind of 1. preparation method of Cordyceps militaris mushroom functional form fermented beverage, it is characterised in that：Include the following steps：S1, carry out Liquid Culture to Cordyceps militaris spawn, prepares Cordyceps militaris zymotic fluid, while carries out Liquid Culture to mushroom strain, Prepare Lentinus edodes fermented liquid；S2, will carry out being mixed to prepare mixed liquor after sterilization of milk with Cordyceps militaris zymotic fluid and Lentinus edodes fermented liquid, and mixed liquor is carried out Defibrination, then with filtered through gauze, obtains filtrate；S3, be warming up to filtrate 90~110 DEG C and keep 10~20min to sterilize, and then cooling is temporary；In a small amount of filtrate to container in S4, taking-up step S3, it is again heated to 116~127 DEG C and keeps 20~40min to go out Bacterium, is subsequently cooled to 40~44 DEG C of inoculation domestications, the strain of access is lactobacillus bulgaricus and streptococcus thermophilus, after domestication Strain expands culture as female bacterium leavening agent；In a small amount of filtrate to container in S5, taking-up step S3, addition sterile water dilutes 50~100 times, is then killed by Pasteur Bacterium method sterilizes postcooling to 22~28 DEG C, adds female bacterium leavening agent and final leavening is made；S6, take out the filtrate in step S3 to fermentation tank, accesses obtained leavening in S5 steps and ferment hair is made Zymotic fluid, inoculum concentration are 4~8%, and temperature is 39~43 DEG C, and fermentation time is 4~12h；Composite sweetener and compound emulsion stabilizer, be added in zymotic fluid by S7, heats up simultaneously high-pressure homogeneous, fermentation drink is made Material；S8, by fermented beverage is filling, packaging, be finally putting into freezer refrigeration and carry out after-ripening.
- 2. the preparation method of Cordyceps militaris mushroom functional form fermented beverage as claimed in claim 1, it is characterised in that：The pupa worm The preparation method of careless zymotic fluid includes the following steps：A, Cordyceps militaris activates：Slant medium is prepared first, and slant medium is then placed into holding temperature 116 in autoclave ~127 DEG C of 20~40min of sterilizing, slant medium is finally cooled down and Cordyceps militaris spawn is added in slant medium, put Enter temperature and be maintained at 5~9d of culture in 18~26 DEG C of constant incubator, the slant medium by following parts by weight material It is made：Potato 150~250 parts (liquors), 15~25 parts of glucose, KH2P041~2 part, 17~21 parts of beef extract, agar 15 ~25 parts, pH is natural, 900~1100 parts of water；B, liquid level-one expands culture：First cell culture medium is prepared first, and first cell culture medium is placed into autoclave and keeps temperature 116~127 DEG C of 20~40min of sterilizing, by the strain access first cell culture medium after obtained activation in above-mentioned steps, are put into temperature Degree is maintained at 4~8d of shaking table culture in 18~26 DEG C of constant incubator, the first cell culture medium by following parts by weight material It is made：18~22 parts of peptone, 27~33 parts of glucose, KH2P040.4~0.3 part, vitaminB10 .01~0.02 part, salt 2~3 parts, 900~1100 parts of water；C, liquid two stage expands culture：Secondary medium is prepared first, and secondary medium is placed into autoclave and keeps temperature 116~127 DEG C of 20~40min of sterilizing, in the obtained strain access secondary medium expanded after cultivating, will connect in above-mentioned steps Kind amount is 3~9%, is put into temperature and is maintained at 4~8d of shaking table culture in 18~26 DEG C of constant incubator, the frequency of oscillation of shaking table It is 80~120r/min, Cordyceps militaris zymotic fluid is made, the preparation of the two level culture is first put into 26~34 parts of base dregs of beans, peptone 30~33 parts, beef extract, ammonium sulfate, each 4~6 parts of soya-bean cake, then plus boiling boil 10 minutes, then add 8~12 parts of white sugar, di(2-ethylhexyl)phosphate 0.9~1.1 part of hydrogen potassium, 0.4~0.6 part of magnesium sulfate, 0.9~1.1 part of gypsum, 9~11 parts of peptone, moisturizing to 900~1100 Part, stirring and dissolving.
- 3. the preparation method of Cordyceps militaris mushroom functional form fermented beverage as claimed in claim 1, it is characterised in that：The mushroom The preparation method of zymotic fluid includes the following steps：A, mushroom strain activates：Activation medium is prepared, activation medium is placed into holding temperature 116~127 in autoclave DEG C sterilizing 15~25min, by mushroom strain access activation medium in, inoculum concentration be 8~12%, in constant temperature at 20~30 DEG C 5~9d of culture in incubator, the concussion frequency of shaking table is 125~175r/min, and the preparation of the activation medium is first put into horse 150~250 parts of bell potato, 1~3 part of sucrose, 1~3 part of peptone, 1~3 part of magnesium sulfate, 1~3 part of potassium dihydrogen phosphate, radish 130~ 170 parts, 18~22 parts of agar boil, filtered fluid is then obtained by filtration and adds water to be supplemented to 900~1100 parts, pH is natural；B, mushroom strain culture：Glucose fermentation culture medium is prepared, glucose fermentation culture medium is placed into autoclave and is kept 116~127 DEG C of 15~25min of sterilizing of temperature, the activation mushroom strain that above-mentioned steps are obtained access glucose fermentation culture medium In, inoculum concentration be 8~12%, ensure pH value be 4.5~6.5, at 20~30 DEG C in shaking table culture in constant incubator 1~ 3d, the frequency of oscillation of shaking table is 125~175r/min, Lentinus edodes fermented liquid is made, the glucose fermentation culture medium by weighing as follows The material of amount part is made：18~22 parts of glucose, 1~3 part of peptone, 0.4~0.6 part of sulphur magnesium, 0~12 part of vitamin B11, phosphorus 0.4~0.6 part of acid dihydride potassium, 0.4~0.6 part of calcium chloride, stirring and dissolving, to 900~1100 parts, it is 5.5 to adjust pH for moisturizing.
- 4. the preparation method of Cordyceps militaris mushroom functional form fermented beverage as claimed in claim 1, it is characterised in that：In step S2 Cordyceps sinensis fermentation liquor, Lentinus edodes fermented liquid, the mixed proportion of milk are 2~6：2~6：6~18.
- 5. the preparation method of Cordyceps militaris mushroom functional form fermented beverage as claimed in claim 1, it is characterised in that：In step S2 Mixed liquor is by colloid mill defibrination, with 100 mesh filter-cloth filterings.
- 6. the preparation method of Cordyceps militaris mushroom functional form fermented beverage as claimed in claim 1, it is characterised in that：In step S4 The ratio of lactobacillus bulgaricus and streptococcus thermophilus is 1:1.
- 7. the preparation method of Cordyceps militaris mushroom functional form fermented beverage as claimed in claim 1, it is characterised in that：In step S7 Composite sweetener includes Semen Coicis syrup and white granulated sugar, and the mass percent that Semen Coicis syrup accounts for zymotic fluid is 0.15~0.5%, white sand The mass percent that sugar accounts for zymotic fluid is 1~2%.
- 8. the preparation method of Cordyceps militaris mushroom functional form fermented beverage as claimed in claim 1, it is characterised in that：In step S7 Compound emulsion stabilizer includes pectin, citric acid and beta-cyclodextrin, and the mass percent that pectin accounts for zymotic fluid is 2~4%, lemon The mass percent that lemon acid accounts for zymotic fluid is 0.01~0.03%, beta-cyclodextrin account for the mass percent of zymotic fluid for 0.02~ 0.03%.
- 9. the preparation method of Cordyceps militaris mushroom functional form fermented beverage as claimed in claim 1, it is characterised in that：In step S7 Zymotic fluid is warming up to homogeneous 1~6 time under 50~70 DEG C and 10~45MPa.
- 10. the preparation method of Cordyceps militaris mushroom functional form fermented beverage as claimed in claim 1, it is characterised in that：Step S8 Middle ripening time is 12~24h.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711231995.2A CN107960470A (en) | 2017-11-30 | 2017-11-30 | A kind of preparation method of Cordyceps militaris mushroom functional form fermented beverage |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711231995.2A CN107960470A (en) | 2017-11-30 | 2017-11-30 | A kind of preparation method of Cordyceps militaris mushroom functional form fermented beverage |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107960470A true CN107960470A (en) | 2018-04-27 |
Family
ID=61999169
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711231995.2A Pending CN107960470A (en) | 2017-11-30 | 2017-11-30 | A kind of preparation method of Cordyceps militaris mushroom functional form fermented beverage |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107960470A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108850176A (en) * | 2018-06-12 | 2018-11-23 | 徐州工程学院 | A kind of Cordyceps militaris spawn fermentation soybean soymilk and preparation method thereof |
| CN114794233A (en) * | 2022-03-18 | 2022-07-29 | 河西学院 | Method for fermenting yoghourt by using Lyophyllum nuciferum mycelium |
Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20070118721A (en) * | 2006-06-13 | 2007-12-18 | 에프이엔바이오텍주식회사 | Ferment-composite using soybean-fluid and functional foods |
| CN101327006A (en) * | 2008-07-14 | 2008-12-24 | 刘晓林 | Technique for processing edible fungus composite health care yoghourt |
| CN103330258A (en) * | 2013-07-17 | 2013-10-02 | 黄雄 | Cordyceps militaris health-care beverage prepared by liquid submerged fermentation and preparation method thereof |
| CN105505992A (en) * | 2016-01-08 | 2016-04-20 | 湖北省农业科学院农产品加工与核农技术研究所 | Method for producing functional food through probiotics compound biological fermentation |
| CN107484935A (en) * | 2017-09-25 | 2017-12-19 | 中国人民武装警察部队工程大学 | A kind of compound lactobacillus-fermencucumber ginseng juice beverage and preparation method thereof |
-
2017
- 2017-11-30 CN CN201711231995.2A patent/CN107960470A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20070118721A (en) * | 2006-06-13 | 2007-12-18 | 에프이엔바이오텍주식회사 | Ferment-composite using soybean-fluid and functional foods |
| CN101327006A (en) * | 2008-07-14 | 2008-12-24 | 刘晓林 | Technique for processing edible fungus composite health care yoghourt |
| CN103330258A (en) * | 2013-07-17 | 2013-10-02 | 黄雄 | Cordyceps militaris health-care beverage prepared by liquid submerged fermentation and preparation method thereof |
| CN105505992A (en) * | 2016-01-08 | 2016-04-20 | 湖北省农业科学院农产品加工与核农技术研究所 | Method for producing functional food through probiotics compound biological fermentation |
| CN107484935A (en) * | 2017-09-25 | 2017-12-19 | 中国人民武装警察部队工程大学 | A kind of compound lactobacillus-fermencucumber ginseng juice beverage and preparation method thereof |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108850176A (en) * | 2018-06-12 | 2018-11-23 | 徐州工程学院 | A kind of Cordyceps militaris spawn fermentation soybean soymilk and preparation method thereof |
| CN114794233A (en) * | 2022-03-18 | 2022-07-29 | 河西学院 | Method for fermenting yoghourt by using Lyophyllum nuciferum mycelium |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103054119B (en) | Preparation method of black garlic fermented beverage | |
| CN102805170B (en) | Black tea fungus beverage as well as preparation technology and application thereof | |
| CN104621662A (en) | Method for preparing functional drink by utilizing radix puerariae-lactic acid bacteria mixed fermentation | |
| CN107125761A (en) | Root of kudzu vine extract, root of kudzu vine Yoghourt and preparation method thereof | |
| CN101263902A (en) | Health care type red date, fresh ginger and lactic acid fermentation beverage and preparation thereof | |
| CN104305427A (en) | Grape and red tea fungus drink | |
| CN106234895A (en) | A kind of Fructus Nitraria schoberi lactacidase fermenting beverage and preparation method thereof | |
| CN102524396A (en) | Stirred type Pleurotus eryngii nutritional yogurt drink and preparation method thereof | |
| CN101828734B (en) | Sweet potato and soybean composite fermented beverage and preparation technology thereof | |
| CN106962925A (en) | Make the composition and its method of ferment | |
| CN104287046B (en) | A kind of take peanut as the bion beverage of primary raw material | |
| CN104068433A (en) | Preparation method of red jujube and castanea mollissima vinegar beverage | |
| CN105211970A (en) | A kind of Momordica grosvenori lactic acid fermentation fruit juice and preparation method thereof | |
| CN103598331B (en) | Rehmannia root functional yoghourt, rehmannia root blueberry functional yoghourt and preparation method thereof | |
| CN107927161A (en) | A kind of preparation process of almond soybean flavored fermented milk | |
| CN105567496B (en) | A kind of thick wine of gold cordyceps sinensis lotus seeds | |
| CN101406214A (en) | Golden fungus yoghourt with nutrition health-care functions and preparation method thereof | |
| CN107960470A (en) | A kind of preparation method of Cordyceps militaris mushroom functional form fermented beverage | |
| CN104450357A (en) | Method for brewing red date wine | |
| CN106262837A (en) | A kind of Fructus Musae Fructus Lycii ferment and preparation method thereof | |
| CN106281919A (en) | A kind of Cordyceps militaris (L.) Link. modulation wine and preparation method thereof | |
| CN103734851B (en) | Method for preparing non-milk probiotic beverage based on aspergillus oryzae culture | |
| CN101125019B (en) | Pholiota adiposa mycelium fermentation vinegar beverage and preparation method | |
| CN108420064A (en) | Preparation method of rose and persimmon composite enzyme | |
| CN104286280A (en) | Pawpaw and red tea fungus drink |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| RJ01 | Rejection of invention patent application after publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20180427 |