CN107957490B - Experimental device for seawater micro-plastic ecological niche in-situ research and using method - Google Patents
Experimental device for seawater micro-plastic ecological niche in-situ research and using method Download PDFInfo
- Publication number
- CN107957490B CN107957490B CN201711147898.5A CN201711147898A CN107957490B CN 107957490 B CN107957490 B CN 107957490B CN 201711147898 A CN201711147898 A CN 201711147898A CN 107957490 B CN107957490 B CN 107957490B
- Authority
- CN
- China
- Prior art keywords
- micro
- plastic
- collection
- net cage
- seawater
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Active
Links
- 229920003023 plastic Polymers 0.000 title claims abstract description 72
- 239000004033 plastic Substances 0.000 title claims abstract description 72
- 239000013535 sea water Substances 0.000 title claims abstract description 39
- 238000011160 research Methods 0.000 title claims abstract description 26
- 238000011065 in-situ storage Methods 0.000 title claims abstract description 20
- 238000000034 method Methods 0.000 title claims abstract description 13
- 230000007613 environmental effect Effects 0.000 claims abstract description 15
- 238000004873 anchoring Methods 0.000 claims abstract description 14
- 229920000426 Microplastic Polymers 0.000 claims abstract description 9
- 239000000463 material Substances 0.000 claims abstract description 9
- 238000002474 experimental method Methods 0.000 claims abstract description 8
- 238000012613 in situ experiment Methods 0.000 claims abstract description 8
- 150000003839 salts Chemical class 0.000 claims abstract description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 52
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 28
- 239000001301 oxygen Substances 0.000 claims description 28
- 229910052760 oxygen Inorganic materials 0.000 claims description 28
- 239000011521 glass Substances 0.000 claims description 23
- 229910001220 stainless steel Inorganic materials 0.000 claims description 21
- 239000010935 stainless steel Substances 0.000 claims description 21
- 238000004458 analytical method Methods 0.000 claims description 10
- 239000007789 gas Substances 0.000 claims description 9
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 8
- 238000005138 cryopreservation Methods 0.000 claims description 6
- 238000007710 freezing Methods 0.000 claims description 5
- 230000008014 freezing Effects 0.000 claims description 5
- 238000005070 sampling Methods 0.000 claims description 5
- 238000005406 washing Methods 0.000 claims description 5
- 229910052757 nitrogen Inorganic materials 0.000 claims description 4
- 238000007789 sealing Methods 0.000 claims description 4
- 239000007788 liquid Substances 0.000 claims description 3
- 238000012544 monitoring process Methods 0.000 claims description 3
- 235000015097 nutrients Nutrition 0.000 claims description 3
- 239000002245 particle Substances 0.000 claims description 3
- 238000003825 pressing Methods 0.000 claims description 2
- 238000010008 shearing Methods 0.000 claims description 2
- VVQNEPGJFQJSBK-UHFFFAOYSA-N Methyl methacrylate Chemical compound COC(=O)C(C)=C VVQNEPGJFQJSBK-UHFFFAOYSA-N 0.000 claims 1
- 229920005372 Plexiglas® Polymers 0.000 claims 1
- 238000011835 investigation Methods 0.000 abstract description 4
- 230000007547 defect Effects 0.000 abstract description 3
- 230000000050 nutritive effect Effects 0.000 abstract description 3
- 241000894006 Bacteria Species 0.000 description 4
- 238000005457 optimization Methods 0.000 description 3
- 238000003860 storage Methods 0.000 description 3
- 230000000813 microbial effect Effects 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 239000000203 mixture Substances 0.000 description 2
- 239000003973 paint Substances 0.000 description 2
- 239000002028 Biomass Substances 0.000 description 1
- 241000282414 Homo sapiens Species 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- 241000607598 Vibrio Species 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 238000005352 clarification Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 244000005706 microflora Species 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- 239000011148 porous material Substances 0.000 description 1
- 238000010223 real-time analysis Methods 0.000 description 1
- 230000001360 synchronised effect Effects 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 231100000027 toxicology Toxicity 0.000 description 1
Images
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/44—Resins; Plastics; Rubber; Leather
- G01N33/442—Resins; Plastics
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The invention provides an experimental device for seawater micro-plastic ecological niche in-situ research and a using method, belonging to the field of marine environment, and comprising an anchoring system, a bearing system, a ballast system and a collecting system; the anchoring system is connected with the bearing system, and the ballast system is connected above the bearing system; the collection system is used for collecting the obtained sample; the invention carries out in-situ experiment by constructing experiment conditions consistent with environmental parameters such as natural seawater temperature, salinity, pH, nutritive salt and the like, and overcomes the defects of uncertainty and complexity of on-site investigation and collection of micro plastic material, shape and size and difficulty in reducing real marine environment under laboratory conditions. Meanwhile, a ballast system in the device realizes the automatic floating and sinking of the bearing system, and is convenient for the putting and collection of samples. In addition, the collection system has good operation coordination on the ship and quick collection, and the application of the device of the method provides important guarantee for analyzing the ecological niche structure characteristics of the micro-plastics and the correlation between the ecological niche structure characteristics and the environmental factors.
Description
Technical Field
The invention belongs to the field of marine environment, and particularly relates to an experimental device for seawater micro-plastic ecological niche in-situ research and a using method thereof.
Background
Plastic pollution has attracted considerable attention from researchers as a global problem with increasing threat to marine life, and is considered to be a significant emerging threat to the current global environmental protection problem. The total financial loss due to the presence of plastics in marine ecosystems is conservatively estimated to be $ 130 million per year, based on two reports published at the time of the first united nations environmental congress. Although plastics pollution has attracted international attention, knowledge about the behaviour of plastics, especially micro-plastics, in marine environments is still developing. Researchers have indicated that plastic chips are one of the most abundant community structures in marine chips. It is now found that marine bacteria including some marine pathogenic bacteria (vibrio, etc.) can adhere to the micro-plastics and continuously reproduce to form a "plastic ball" (plastics), which is transferred to organisms with higher nutrition level through food chain to cause harm to marine organisms and even human beings, so that the clarification of the ecological characteristics of the micro-plastics is an important precondition for researching the toxic effect of the micro-plastics and an important research direction in the ecological toxicology research of the micro-plastics.
In a semi-closed environment in a port, researchers observed the formation of microbial biofilms for only one week, after which the abundance of bacteria increased and the taxonomic composition of bacteria gradually changed as the biomass of bacteria increased. In addition, studies have also emphasized that plastic pellet microflora structural composition is primarily related to the season of sampling, geographic location, and characteristics of the plastic. At present, the research on the microbial community structure on the micro-plastic is based on the research on the field sampling investigation, and the complexity and uncertainty of the ecological niche analysis caused by the diversity and the asynchronism of the micro-plastic samples collected by the field collection field investigation are difficult to analyze the dominant environmental factors influencing the ecological niche characteristics of the micro-plastic. At present, the experimental device for seawater micro-plastic ecological niche in-situ research has no development of related work at home, and no related report exists at abroad. The invention relates to an experimental device for seawater micro-plastic ecological niche in-situ research and a using method thereof.A micro-plastic is ensured to be fully contacted with marine microorganisms under normal ocean current environment by constructing experimental conditions consistent with marine environmental parameters such as natural seawater temperature, salt, PH, dissolved oxygen, nutritive salt and the like and utilizing an anchoring system, a bearing system, a ballast tank system and a collecting system, so that the enrichment of the micro-plastic on the marine microorganisms is realized, a stable ecological niche is formed, then the distribution and enrichment conditions of various procaryon and eucaryon in the micro-plastic ecological niche are sampled and analyzed in real time, the synchronous putting and real-time analysis of the same type of micro-plastic are realized, and possibility is provided for later analysis of main environmental factors influencing the micro-plastic ecological niche characteristics.
Disclosure of Invention
The invention aims to provide an experimental device for in-situ research on a seawater micro-plastic ecological niche and a using method thereof, and the device is used for realizing in-situ investigation and research on the micro-plastic ecological niche in a marine environment.
The invention is realized by the following technical scheme:
an experimental facility for seawater micro-plastic ecological niche in-situ research comprises an anchoring system, a bearing system, a ballast system and a collecting system; the anchoring system is connected with the bearing system, and the ballast system is connected above the bearing system; the collection system is used for collecting the obtained sample;
the anchoring system comprises an anchor, an anchor chain and a rope, wherein two ends of the anchor chain are respectively connected with the anchor and the rope, and the rope is connected with the bearing system;
the bearing system comprises an outer net cage and an inner net cage; the inner net cage is arranged in the outer net cage;
the ballast system includes that one is connected at the organic glass seal box who bears the weight of the system top, be equipped with water intaking valve and drain valve on the organic glass seal box, be provided with the oxygen cylinder in the organic glass seal box, the solenoid valve, first electric wire, control panel and second electric wire, the organic glass seal box is provided with the electromagnetic switch who connects the cursory outward, the gas outlet department at the oxygen cylinder is fixed to the solenoid valve, by the switching of electromagnetic switch control oxygen cylinder gas outlet, first electric wire connection solenoid valve and control panel, control panel is connected to second electric wire one end, electromagnetic switch is connected to the other end, through electromagnetic switch's opening or closing, help to bear the weight.
As optimization, the outer cylinder mould is a rectangular stainless steel cylinder mould with the diameter of 80cm multiplied by 60cm, the aperture of the outer cylinder mould is 6cm, and a cylinder door capable of being freely opened and closed is arranged on the rectangular outer cylinder mould; interior cylinder type cylinder mould that the cylinder type mould made for stainless steel material, interior mould diameter is 8cm, and interior mould height is 15cm, and interior mould aperture is 0.5mm, 1mm, 2mm or 5mm, also is provided with a lid that can freely open and shut in the outside of interior mould.
As optimization, the specification of the organic glass sealing box is 80cm in length, 60cm in width and 60cm in height.
As optimization, the collecting system comprises a supporting plate, a collecting net rack and a supporting frame; the support frame is a round table type stainless steel frame, the diameter of the bottom of the support frame is 30cm, the diameter of the top of the support frame is 10cm, the height of the support frame is 30cm, a stainless steel support plate which inclines downwards is arranged at the upper end inside the support frame, and the support plate plays a role in supporting the inner mesh cage when a sample is collected; be provided with the stainless steel in the support frame and collect the rack, collect the rack and be the cylinder type, collect the net and freely place on collecting the rack, the aperture of collecting the net is 0.2 mm.
The invention also provides a use method of the experimental device for the seawater micro-plastic ecological niche in-situ research, which comprises the following steps:
(1) opening an inner net cage of the bearing system, respectively putting micro plastics with different particle sizes and materials to be researched into the inner net cage, then closing a cover of the inner net cage, opening a net door of a rectangular outer net cage, putting a cylindrical inner net cage into the rectangular outer net cage, and closing the net door of the outer net cage;
(2) fixing the bearing system and the ballast system near a sea area survey station site by adopting an anchor, an anchor chain and a rope;
(3) opening a water inlet valve of the ballast system, closing a water outlet valve, filling seawater into an organic glass sealed tank of the ballast system, immersing the bearing system into the seawater surface for 0.4-0.6m under the pressure action of the ballast system, stopping filling the organic glass sealed tank with the seawater, closing the water inlet valve, and starting an in-situ experiment of the study on the ecological level of the micro-plastics;
(4) designing sampling time according to experimental needs, when collecting samples, firstly pressing down an electromagnetic switch connected with a buoy, inputting electromagnetic signals to an electromagnetic valve through a control panel in a ballast system, after the electromagnetic valve receives the signals, opening an air bottle, releasing oxygen from the oxygen bottle, increasing the buoyancy of the ballast system, and enabling a bearing system to float out of the water surface; then, opening a net door on the side surface of the outer net cage, and taking out the inner net cage; after closing the outer net cage net door, opening the water inlet valve to continuously immerse the bearing system into the water for 0.4-0.6m, then closing the water inlet valve, and continuously performing the in-situ experiment; after repeated times, the organic glass sealed box is filled with seawater and oxygen, a water outlet valve needs to be opened to discharge the seawater and the gas in the organic glass sealed box, an oxygen cylinder is replaced, then the water outlet valve is closed, a water inlet valve is opened again, the bearing system is pressed underwater, and subsequent experiments are continued;
(5) opening the cover of the inner cylinder mould and collecting the micro plastic; firstly, laying a collection net on a collection net rack, clamping by using a clamp, then opening a cover on the side surface of an inner net cage, placing the collection net on a support plate of a stainless steel support frame of a collection system, washing the outer side of the inner net cage by using clean seawater filtered in advance, rapidly washing the micro-plastic in the inner net cage into the collection net under the action of water flow, then shearing the bottom of the collection net, placing the micro-plastic gathered at the bottom of the collection net and the sheared collection net into a cryopreservation tube, writing a mark on the cryopreservation tube after sealing, rapidly placing the cryopreservation tube into a liquid nitrogen tank for quick freezing, and bringing a collected micro-plastic sample back to a laboratory for biological analysis;
(6) and (3) monitoring the water quality characteristics of the micro-plastic throwing area while collecting samples, wherein the water quality characteristics comprise temperature, dissolved oxygen, pH and salinity, collecting a certain volume of water sample, bringing the water sample back to a laboratory for analyzing the content of nutrient salts, combining the measured water quality indexes with biological characteristic analysis results in different types of micro-plastics, performing response analysis on the micro-plastic ecological niche structural characteristics and the culture area environmental factors, calculating the type of the micro-plastics and the correlation between the environmental factors and the micro-plastic ecological niche structural characteristics, and determining the leading factors influencing the structural characteristics.
The seawater micro-plastic ecological niche in-situ research experiment device and the use method thereof have the advantages that through establishing the experiment conditions consistent with the environmental parameters of natural seawater temperature, salinity, pH, nutritive salt and the like, and utilizing the anchoring system, the bearing system and the ballast system to place different types of micro-plastics in the marine environment for in-situ experiment, the determinacy of research objects and research conditions and the consistency of experiment time are ensured, and the defects that the uncertainty and the complexity of the material, the shape and the size of the collected micro-plastics are surveyed and collected on site and the real marine environment is difficult to restore under the laboratory condition are overcome. Meanwhile, a ballast system in the device realizes the automatic floating and sinking of the bearing system, and is convenient for the putting and collection of samples. In addition, the collection system firmly supports the inner net cage on the support frame, the defect of poor operation coordination on the ship is overcome, the rapid collection of the micro-plastic samples is realized, and the application of the in-situ device provides important guarantee for analyzing the ecological niche characteristics of the micro-plastic and the correlation between the ecological niche characteristics and the environmental factors.
Drawings
The experimental device for seawater micro-plastic ecological niche in-situ research and the using method thereof are further described by the following embodiments in combination with the attached drawings:
fig. 1 is a schematic structural view of the anchoring system, load carrying system and ballast system of the present invention: 1-anchor, 2-anchor chain, 3-rope, 4-outer cage, 5-inner cage, 6 ballast tank, 7-built-in oxygen cylinder, 8-electromagnetic valve, 9-first electric wire, 10-control panel, 11-second electric wire, 12-electromagnetic switch, 13-float, 14-water inlet valve and 15 water outlet valve.
FIG. 2 is a schematic diagram of the collection system in the experimental apparatus of the present invention: 16-supporting plate, 17-collecting net, 18-collecting net rack and 19-supporting frame.
Detailed Description
Example 1
An experimental device for seawater micro-plastic ecological niche in-situ research is shown in figure 1 and comprises an anchoring system, a bearing system, a ballast system and a collecting system; the anchoring system is connected with the bearing system, and the ballast system is fixedly connected above the bearing system; the collection system is used for collecting the obtained sample;
the anchoring system comprises an anchor 1, an anchor chain 2 and a rope 3, wherein two ends of the anchor chain are respectively connected with the anchor and the rope, and the rope is connected with the bearing system;
the bearing system comprises an outer net cage and an inner net cage; the inner net cage is arranged in the outer net cage; the outer cylinder mould is a rectangular stainless steel cylinder mould with the diameter of 80cm multiplied by 60cm, the aperture of the outer cylinder mould is 6cm, and a cylinder gate capable of being freely opened and closed is arranged on the rectangular outer cylinder mould; interior cylinder type cylinder mould that the cylinder type mould for stainless steel material made, interior mould diameter is 8cm, and interior mould height is 15cm, applies paint with a brush anticorrosive paint, and interior mould aperture is 0.5mm, also can set to 1mm, 2mm or 5mm, also is provided with a lid that can open in the outside of interior mould.
The ballast system comprises an organic glass seal box 6 connected above the bearing system, and the specification of the organic glass seal box is 80cm long, 60cm wide and 60cm high; be equipped with water intaking valve 14 and drain valve 15 on the organic glass seal box, be provided with oxygen cylinder 7, solenoid valve 8, first electric wire 9, control panel 10 in the organic glass seal box, second electric wire 11 and external electromagnetic switch 12 of connecting float 13, the gas outlet department at the oxygen cylinder is fixed to the solenoid valve to the switching of control oxygen cylinder gas outlet, solenoid valve 8 and control panel 10 are connected to first electric wire 9, 11 one end connection control panels 10 of second electric wire, and electromagnetic switch 12 is connected to the other end, the oxygen cylinder volume is 5 liters, through electromagnetic switch's opening or closing, assists the system of bearing come-up and sink.
The collecting system is shown in fig. 2 and comprises a supporting frame, a collecting net rack and a supporting plate; the support frame is in a round table shape, the bottom and the top of the support frame are both round stainless steel rings, the diameter of the bottom of the support frame is 30cm, the diameter of the top of the support frame is 10cm, the height of the support frame is 30cm, and the upper stainless steel ring and the lower stainless steel ring are connected through six stainless steel plates. The supporting plate is welded on the side face of the stainless steel plate of the supporting frame, is close to the upper round table and is used for supporting the inner mesh cage. Be provided with the collection rack in the support frame, it is a porous cylindric structure (aperture 3cm) that the collection rack also comprises stainless steel material, and collection net can be accepted at its top, and the aperture on it can let the sea water that washes down flow out from collection device rapidly. The pore size of the collection mesh was 0.2 mm.
The use method of the experimental device for the seawater micro-plastic ecological niche in-situ research comprises the following steps:
(1) firstly, opening a cover of an inner cylinder mould of a bearing system, respectively putting micro-plastics with different particle sizes and materials to be researched into different inner cylinder moulds, then closing the cover of the inner cylinder mould, opening a rectangular outer cylinder mould, putting the inner cylinder mould into the rectangular outer cylinder mould, and closing a net door of the outer cylinder mould.
(2) Anchors, chains and lines are used to secure the load bearing system and ballast system near the marine survey station (longitude: 122 deg. 28.800 ', latitude 37 deg. 2.436')).
(3) And opening a water inlet valve of the ballast system, filling seawater into the ballast system, immersing the bearing system into the seawater about 0.5m below the water surface under the pressure action of the ballast system, stopping filling the water into the ballast system, closing the water inlet valve of the ballast system, and starting an in-situ experiment of the micro-plastic ecological niche research.
(4) Sampling time 7d, 14d, 28d, 56d and 112d are designed according to experiment needs, when a sample is collected, an overwater electromagnetic switch connected with a buoy is pressed down, an electromagnetic signal is input to an electromagnetic valve connected to an air outlet of an oxygen cylinder through a control board in a ballast system, the electromagnetic valve opens the air cylinder after receiving the signal, the oxygen cylinder releases oxygen, buoyancy of the ballast system is increased, and a bearing system floats out of the water. Then, the net door on the side surface of the outer net cage is opened, and the inner net cage is taken out. And after closing the outer net cage, opening the water inlet valve to continuously immerse the bearing system into the water for 0.5m, closing the water inlet valve, and continuously performing the in-situ experiment. After repeated for many times, the organic glass sealed box of the ballast system is filled with seawater and oxygen, the water outlet valve on the organic glass sealed box is opened to discharge the seawater and the gas, the oxygen cylinder is replaced, then the water inlet valve is opened again, the bearing system is pressed underwater, and then the subsequent experiment is continued.
(5) The lid of the inner cylinder mould was opened and the micro-plastic was collected. Firstly, a collecting net is laid on a collecting net rack and clamped by a clamp, then a cover of an inner net cage is opened, the collecting net rack is placed on a supporting plate of a stainless steel supporting frame of a collecting system, clean seawater which is filtered in advance is used for washing the outer side of the inner net cage, the micro plastic of the inner net cage is quickly washed into the collecting net which is arranged in the supporting frame in a built-in mode under the action of water flow, then a net bottom of the collecting net is cut off, the micro plastic which is wrapped in the collecting net bottom and gathered at the net bottom and the cut net are placed into a freezing storage pipe together, after the sealing is well carried out, a mark is written on the freezing storage pipe, a liquid nitrogen tank is quickly placed into the freezing storage pipe, and a collected micro plastic sample is taken back to.
(6) The method comprises the steps of collecting samples, monitoring water quality characteristics of a micro-plastic throwing area, including temperature, dissolved oxygen, pH and salinity, collecting a certain volume of water sample, bringing the water sample back to a laboratory for analyzing the content of nutrient salts (inorganic nitrogen and phosphate), combining the measured water quality indexes with biological characteristic analysis results in different types of micro-plastics, performing response analysis on the ecological niche characteristics of the micro-plastics and environmental factors in a culture area, calculating the correlation between the types of the micro-plastics and the ecological niche structural characteristics (diversity, richness and the like) of the environmental factors and the micro-plastics, and determining the leading factors influencing the ecological niche structural characteristics of the micro-plastics.
Claims (5)
1. An experimental facility for seawater micro-plastic ecological niche in-situ research is characterized by comprising an anchoring system, a bearing system, a ballast system and a collecting system; the anchoring system is connected with the bearing system, and the ballast system is connected above the bearing system; the collection system is used for collecting the obtained sample;
the anchoring system comprises an anchor, an anchor chain and a rope, wherein two ends of the anchor chain are respectively connected with the anchor and the rope, and the rope is connected with the bearing system;
the bearing system comprises an outer net cage and an inner net cage; the inner net cage is arranged in the outer net cage;
the ballast system includes that one is connected at the organic glass seal box who bears the weight of the system top, be equipped with water intaking valve and drain valve on the organic glass seal box, be provided with the oxygen cylinder in the organic glass seal box, the solenoid valve, first electric wire, control panel and second electric wire, the organic glass seal box is provided with the electromagnetic switch who connects the cursory outward, the gas outlet department at the oxygen cylinder is fixed to the solenoid valve, by the switching of electromagnetic switch control oxygen cylinder gas outlet, first electric wire connection solenoid valve and control panel, control panel is connected to second electric wire one end, electromagnetic switch is connected to the other end, through electromagnetic switch's opening or closing, help to bear the weight.
2. The experimental device for in-situ research on seawater micro-plastic ecological niches as claimed in claim 1, wherein the outer cylinder mould is a rectangular stainless steel cylinder mould of 80cm x 60cm, the aperture of the outer cylinder mould is 6cm, and a face of a mesh door capable of being freely opened and closed is arranged on the rectangular outer cylinder mould; interior cylinder type cylinder mould that the cylinder type mould made for stainless steel material, interior mould diameter is 8cm, and interior mould height is 15cm, and interior mould aperture is 0.5mm, 1mm, 2mm or 5mm, also is provided with a lid that can freely open and shut in the outside of interior mould.
3. The experimental facility for in-situ research on seawater micro-plastic niches as claimed in claim 1, wherein the plexiglass sealed box has a length of 80cm, a width of 60cm and a height of 60 cm.
4. The experimental facility for in-situ research on seawater micro-plastic niches as claimed in claim 1, wherein the collection system comprises a support plate, a collection net rack and a support frame; the support frame is a round table type stainless steel frame, the diameter of the bottom of the support frame is 30cm, the diameter of the top of the support frame is 10cm, the height of the support frame is 30cm, a stainless steel support plate which inclines downwards is arranged at the upper end inside the support frame, and the support plate plays a role in supporting the inner mesh cage when a sample is collected; be provided with the stainless steel in the support frame and collect the rack, collect the rack and be the cylinder type, collect the net and freely place on collecting the rack, the aperture of collecting the net is 0.2 mm.
5. The use method of the experimental facility for the in-situ research on the seawater micro-plastic niche as claimed in claim 4 is characterized by comprising the following steps:
(1) opening an inner net cage of the bearing system, respectively putting micro plastics with different particle sizes and materials to be researched into the inner net cage, then closing a cover of the inner net cage, opening a net door of a rectangular outer net cage, putting a cylindrical inner net cage into the rectangular outer net cage, and closing the net door of the outer net cage;
(2) fixing the bearing system and the ballast system near a sea area survey station site by adopting an anchor, an anchor chain and a rope;
(3) opening a water inlet valve of the ballast system, closing a water outlet valve, filling seawater into an organic glass sealed tank of the ballast system, immersing the bearing system into the seawater surface for 0.4-0.6m under the pressure action of the ballast system, stopping filling the organic glass sealed tank with the seawater, closing the water inlet valve, and starting an in-situ experiment of the study on the ecological level of the micro-plastics;
(4) designing sampling time according to experimental needs, when collecting samples, firstly pressing down an electromagnetic switch connected with a buoy, inputting electromagnetic signals to an electromagnetic valve through a control panel in a ballast system, after the electromagnetic valve receives the signals, opening an air bottle, releasing oxygen from the oxygen bottle, increasing the buoyancy of the ballast system, and enabling a bearing system to float out of the water surface; then, opening a net door on the side surface of the outer net cage, and taking out the inner net cage; after closing the outer net cage net door, opening the water inlet valve to continuously immerse the bearing system into the water for 0.4-0.6m, then closing the water inlet valve, and continuously performing the in-situ experiment; after repeated times, the organic glass sealed box is filled with seawater and oxygen, a water outlet valve needs to be opened to discharge the seawater and the gas in the organic glass sealed box, an oxygen cylinder is replaced, then the water outlet valve is closed, a water inlet valve is opened again, the bearing system is pressed underwater, and subsequent experiments are continued;
(5) opening the cover of the inner cylinder mould and collecting the micro plastic; firstly, laying a collection net on a collection net rack, clamping by using a clamp, then opening a cover on the side surface of an inner net cage, placing the collection net on a support plate of a stainless steel support frame of a collection system, washing the outer side of the inner net cage by using clean seawater filtered in advance, rapidly washing the micro-plastic in the inner net cage into the collection net under the action of water flow, then shearing the bottom of the collection net, placing the micro-plastic gathered at the bottom of the collection net and the sheared collection net into a cryopreservation tube, writing a mark on the cryopreservation tube after sealing, rapidly placing the cryopreservation tube into a liquid nitrogen tank for quick freezing, and bringing a collected micro-plastic sample back to a laboratory for biological analysis;
and (3) monitoring the water quality characteristics of the micro-plastic throwing area while collecting samples, wherein the water quality characteristics comprise temperature, dissolved oxygen, pH and salinity, collecting a certain volume of water sample, bringing the water sample back to a laboratory for analyzing the content of nutrient salts, combining the measured water quality indexes with biological characteristic analysis results in different types of micro-plastics, performing response analysis on the micro-plastic ecological niche structural characteristics and the culture area environmental factors, calculating the type of the micro-plastics and the correlation between the environmental factors and the micro-plastic ecological niche structural characteristics, and determining the leading factors influencing the structural characteristics.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711147898.5A CN107957490B (en) | 2017-11-17 | 2017-11-17 | Experimental device for seawater micro-plastic ecological niche in-situ research and using method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711147898.5A CN107957490B (en) | 2017-11-17 | 2017-11-17 | Experimental device for seawater micro-plastic ecological niche in-situ research and using method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN107957490A CN107957490A (en) | 2018-04-24 |
| CN107957490B true CN107957490B (en) | 2020-04-03 |
Family
ID=61963798
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711147898.5A Active CN107957490B (en) | 2017-11-17 | 2017-11-17 | Experimental device for seawater micro-plastic ecological niche in-situ research and using method |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107957490B (en) |
Families Citing this family (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109238914B (en) * | 2018-11-23 | 2023-09-22 | 中国科学院南京地理与湖泊研究所 | Research device and analysis method for influence of organisms with different particle sizes on degradation of organic matters |
| CN110286004A (en) * | 2019-07-11 | 2019-09-27 | 叶建锋 | The acquisition method and device of a kind of micro- plastics sedimentation of atmosphere for different height |
| CN113552030B (en) * | 2021-07-19 | 2023-01-31 | 国家海洋技术中心 | Expendable marine micro-plastic concentration field measurement device and method |
| CN115468882A (en) * | 2022-09-15 | 2022-12-13 | 中国水利水电科学研究院 | Indoor simulation device for measuring sedimentation rate of microbial plastic biofilm in water body |
Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN203256669U (en) * | 2013-05-09 | 2013-10-30 | 长江水利委员会长江科学院 | Multifunctional suspension type river ecology enclosure experiment device |
| CN105651748A (en) * | 2016-01-18 | 2016-06-08 | 南京大学 | Method for quantitatively analyzing enrichment and distribution of micro plastics in aquatic organisms |
| CN205813229U (en) * | 2016-05-25 | 2016-12-21 | 滕宗坤 | A kind of flood control aquaculture net cage |
| CN106769356A (en) * | 2016-12-05 | 2017-05-31 | 北京师范大学 | A kind of micro- plastics extraction element and its method for preventing sample by microfibre pollution |
| CN106969941A (en) * | 2017-04-14 | 2017-07-21 | 宁波大学 | Micro- plastics harvester and its acquisition method in a kind of superficial water |
| CN206565020U (en) * | 2017-03-14 | 2017-10-20 | 中国水产科学研究院黄海水产研究所 | Tangleweed original position experimental simulation system |
-
2017
- 2017-11-17 CN CN201711147898.5A patent/CN107957490B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN203256669U (en) * | 2013-05-09 | 2013-10-30 | 长江水利委员会长江科学院 | Multifunctional suspension type river ecology enclosure experiment device |
| CN105651748A (en) * | 2016-01-18 | 2016-06-08 | 南京大学 | Method for quantitatively analyzing enrichment and distribution of micro plastics in aquatic organisms |
| CN205813229U (en) * | 2016-05-25 | 2016-12-21 | 滕宗坤 | A kind of flood control aquaculture net cage |
| CN106769356A (en) * | 2016-12-05 | 2017-05-31 | 北京师范大学 | A kind of micro- plastics extraction element and its method for preventing sample by microfibre pollution |
| CN206565020U (en) * | 2017-03-14 | 2017-10-20 | 中国水产科学研究院黄海水产研究所 | Tangleweed original position experimental simulation system |
| CN106969941A (en) * | 2017-04-14 | 2017-07-21 | 宁波大学 | Micro- plastics harvester and its acquisition method in a kind of superficial water |
Non-Patent Citations (1)
| Title |
|---|
| 悬浮物对中国明对虾幼体急性毒性效应研究;张艳等;《水产科学》;20170228;第36卷(第1期);第42-47页 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN107957490A (en) | 2018-04-24 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN107957490B (en) | Experimental device for seawater micro-plastic ecological niche in-situ research and using method | |
| CN111477084B (en) | Deep sea cold spring ecosystem formation evolution simulation system and method | |
| CN102012246B (en) | Device for measuring in-situ flow rate change of marine cold seep gas seepage | |
| CN209242762U (en) | A kind of ecological floating island coupling processing device for polluted water body intensive treatment | |
| KR20180085895A (en) | Mesocosm simulation system to test the natural recovery ability of oil contaminated sediments | |
| CN201681072U (en) | City stagnant riverway sediment pollution releasing and inhibition research device | |
| CN202143383U (en) | In-situ cultivation barrel for phytoplankton | |
| CN212134230U (en) | Water layering collection system | |
| CN209673611U (en) | Chloride Attack simulator under a kind of Wet/Dry Cyclic Conditions | |
| CN219391759U (en) | Micro-plastic natural aging in-situ test device in field water environment | |
| WO2023173496A1 (en) | Membrane bioreactor and online environmental parameter measurement system in deep-sea cold spring region | |
| CN114436404B (en) | Sediment normal position bioremediation equipment | |
| CN115343141A (en) | Full-section multi-size micro-plastic synchronous enrichment device and method suitable for small river water body | |
| Blackwood et al. | Exfiltration from sewers-is it a serious problem? | |
| CN211825172U (en) | Sewage discharge sampling detection equipment for marine environmental protection | |
| CN204824892U (en) | Diatom that grows nonparasitically upon another plant is cultivateed and collection system | |
| CN201867402U (en) | Typical pollutant migration device for wet land | |
| CN209911071U (en) | Aquatic plant root system secretion normal position collection device | |
| CN204939094U (en) | A kind of large-scale Hu Ku waters raw bio-artificial matrix device | |
| CN111693438A (en) | Salty and fresh water alternating constant head seepage device and method thereof | |
| McLaughlin et al. | A new sampler for stratified lagoon chemical and microbiological assessments | |
| CN202666895U (en) | Floated on-spot enclosure experimental system | |
| Fadly et al. | Groundwater quality assessment in Jakarta Capital region for the safe drinking water | |
| CN214584218U (en) | Pool waste water sampler | |
| CN102704436A (en) | Suspension type field enclosure experiment system and installation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |