CN107957490A - The experimental provision and application method of the micro- plastics ecological niche on-spot study of seawater - Google Patents
The experimental provision and application method of the micro- plastics ecological niche on-spot study of seawater Download PDFInfo
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- CN107957490A CN107957490A CN201711147898.5A CN201711147898A CN107957490A CN 107957490 A CN107957490 A CN 107957490A CN 201711147898 A CN201711147898 A CN 201711147898A CN 107957490 A CN107957490 A CN 107957490A
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- 229920003023 plastic Polymers 0.000 title claims abstract description 77
- 239000004033 plastic Substances 0.000 title claims abstract description 77
- 239000013535 sea water Substances 0.000 title claims abstract description 36
- 238000000034 method Methods 0.000 title claims abstract description 11
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 23
- 238000002474 experimental method Methods 0.000 claims abstract description 15
- 238000004873 anchoring Methods 0.000 claims abstract description 14
- 238000011065 in-situ storage Methods 0.000 claims abstract description 10
- 239000000463 material Substances 0.000 claims abstract description 8
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 27
- 239000001301 oxygen Substances 0.000 claims description 27
- 229910052760 oxygen Inorganic materials 0.000 claims description 27
- 239000011521 glass Substances 0.000 claims description 25
- 229910001220 stainless steel Inorganic materials 0.000 claims description 20
- 239000010935 stainless steel Substances 0.000 claims description 20
- 239000007789 gas Substances 0.000 claims description 14
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 8
- 238000005138 cryopreservation Methods 0.000 claims description 4
- 230000005611 electricity Effects 0.000 claims description 4
- 238000011010 flushing procedure Methods 0.000 claims description 4
- 229910052757 nitrogen Inorganic materials 0.000 claims description 4
- 238000013461 design Methods 0.000 claims description 3
- 230000000694 effects Effects 0.000 claims description 3
- 239000007788 liquid Substances 0.000 claims description 3
- 235000015097 nutrients Nutrition 0.000 claims description 3
- 238000001914 filtration Methods 0.000 claims description 2
- 235000015110 jellies Nutrition 0.000 claims description 2
- 239000008274 jelly Substances 0.000 claims description 2
- 238000012360 testing method Methods 0.000 claims description 2
- 238000011835 investigation Methods 0.000 abstract description 4
- 150000003839 salts Chemical class 0.000 abstract description 4
- 230000000050 nutritive effect Effects 0.000 abstract description 3
- 230000007613 environmental effect Effects 0.000 abstract description 2
- 238000011160 research Methods 0.000 description 6
- 241000894006 Bacteria Species 0.000 description 3
- 238000005457 optimization Methods 0.000 description 3
- 238000010586 diagram Methods 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 238000005070 sampling Methods 0.000 description 2
- 238000007789 sealing Methods 0.000 description 2
- 239000002028 Biomass Substances 0.000 description 1
- MYMOFIZGZYHOMD-UHFFFAOYSA-N Dioxygen Chemical compound O=O MYMOFIZGZYHOMD-UHFFFAOYSA-N 0.000 description 1
- 229910019142 PO4 Inorganic materials 0.000 description 1
- 241000607598 Vibrio Species 0.000 description 1
- 206010047400 Vibrio infections Diseases 0.000 description 1
- 238000013459 approach Methods 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 230000001680 brushing effect Effects 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 229910001882 dioxygen Inorganic materials 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000000556 factor analysis Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 239000012634 fragment Substances 0.000 description 1
- 230000000813 microbial effect Effects 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 239000003973 paint Substances 0.000 description 1
- 244000052769 pathogen Species 0.000 description 1
- NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 1
- 239000010452 phosphate Substances 0.000 description 1
- 230000007096 poisonous effect Effects 0.000 description 1
- 238000003825 pressing Methods 0.000 description 1
- 230000035807 sensation Effects 0.000 description 1
- 230000001360 synchronised effect Effects 0.000 description 1
- 231100000027 toxicology Toxicity 0.000 description 1
- 230000001228 trophic effect Effects 0.000 description 1
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/44—Resins; Plastics; Rubber; Leather
- G01N33/442—Resins; Plastics
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Sampling And Sample Adjustment (AREA)
Abstract
The present invention provides a kind of experimental provision and application method of the micro- plastics ecological niche on-spot study of seawater, belongs to marine environment field, it includes anchoring system, bearing system, ballasting system and collection system；Anchoring system connects bearing system, and ballasting system is connected to above bearing system；Collection system is used to collect the sample obtained；The present invention is by building the experiment condition consistent with the environmental parameter such as nature seawater water Inversion phenomenon, pH and nutritive salt, carry out experiment in situ, the shortcomings that overcoming field investigation and collect micro- plastic material, the uncertainty and complexity of shapes and sizes, and being difficult to reduce true marine environment under laboratory condition.Meanwhile the ballasting system in the device realizes the automatic of bearing system and floats and sink, and facilitates the dispensing and collection of sample.In addition, operational coordination is good on collection system ship, collect quickly, the application of this law invention device is the micro- plastics ecological niche architectural feature of analysis, and its provides important leverage with envirment factor correlation.
Description
Technical field
The invention belongs to marine environment field, the experiment more particularly to a kind of micro- plastics ecological niche on-spot study of seawater fills
Put and application method.
Background technology
Global problem of the plastic pollution as the getting worse that threatens to marine organisms, has caused the pole of researcher
Big concern, it is considered as the important emerging threats of World Environment protection problem.According to big in first united nations environment
Issued when can open two parts reports, the overall financial loss in marine ecosystems caused by the presence of plastics, conservative estimation
Annual 13000000000 dollars.Although plastic pollution has caused international concern, on the especially micro- plastics of plastics in marine environment
The understanding of behavior still developing.Researcher once pointed out that plastic flakes were one kind that structure of community is most abundant in the fragment of ocean.Mesh
Preceding discovery marine bacteria, which includes some ocean pathogens (vibrios etc.), can be attached on micro- plastics, and constantly breeding forms one
" baton round " (Plastisphere), the organism by food chain transport to higher trophic level, to the marine organisms even mankind
Damage, therefore it is to study the important prerequisite of micro- plastic biologic poisonous effect to make micro- plastics niche characteristics clear, and it is micro-
A highly important research direction in the research of plastics ecological toxicology.
In the semiclosed environment at harbour, researcher only observes the formation of microbial biofilm, Zhi Houxi once week
Bacterium richness increases, and with the increase of bacterial biomass, the classification composition of bacterium gradually changes.In addition, research also emphasizes
The characteristic that baton round biological community structure forms the mainly season with sampling, geographical location and plastics is related.At present on micro-
The research of biological community structure is all based on the research in terms of field sampling investigation on plastics, and on-site collection field investigation is adopted
Collect micro- plastic sample diversity and asynchronism caused by analysis of Niche complexity and uncertainty, it is difficult to analyzing influence
The leading environment factor of micro- plastics niche characteristics.At present, the experimental provision on the micro- plastics ecological niche on-spot study of seawater exists
The domestic development there has been no related work, foreign countries do not have relevant report yet.The micro- plastics ecological niche on-spot study of seawater of the present invention
Experimental provision and its application method, by build and nature seawater water temperature, salt all, the marine environment such as PH, dissolved oxygen, nutritive salt joins
The consistent experiment condition of number, using anchoring system, bearing system, vessel ballast system and collection system, ensures micro- plastics normal
Come into full contact with ocean current environment with marine microorganism, realize its enrichment to marine microorganism, form stable ecology
Position, then various protokaryons and Eukaryotic distribution and enrichment condition in the micro- plastics ecological niche of real time sample analysis, realize phase
The synchronous of the micro- plastics of same type is launched and analysis in real time, and for post analysis influence the main environments of micro- plastics niche characteristics because
Element provides possibility.
The content of the invention
The technical problem to be solved in the present invention is the experimental provision for providing a kind of micro- plastics ecological niche on-spot study of seawater
And application method, realize that the in situ of micro- plastics ecological niche is investigated in marine environment using apparatus of the present invention.
The present invention is achieved by the following technical solution:
A kind of experimental provision of the micro- plastics ecological niche on-spot study of seawater, it includes anchoring system, bearing system, ballast system
System and collection system；Anchoring system connects bearing system, and ballasting system is connected to above bearing system；Collection system is used to collect
The sample of acquisition；
The anchoring system includes anchor, anchor chain and rope, and anchor chain both ends connect anchor and rope, rope connection carrying system respectively
System；
The bearing system includes outer cylinder mould and interior cylinder mould；Interior cylinder mould is placed in outer cylinder mould；
The ballasting system includes an organic glass seal box being connected to above bearing system, organic glass seal box
Inlet valve and drain valve are equipped with, oxygen cylinder, solenoid valve, the first electric wire, control panel and are provided with organic glass seal box
Two electric wires, organic glass seal box are provided outside the electromagnetic switch of connection buoy, and solenoid valve is fixed at the gas outlet of oxygen cylinder,
By the opening and closing of electromagnetic switch control oxygen cylinder gas outlet, the first electric wire connection solenoid valve and control panel, the connection of second electric wire one end
Control panel, other end connection electromagnetic switch, opening or closing by electromagnetic switch, assists bearing system to float or sink.
As an optimization, the outer cylinder mould is a 80cm × 60cm × 60cm rectangle stainless steel cylinder moulds, and outer cylinder mould aperture is
6cm, rectangle are equipped with the veil door for being capable of free-open-close outside above cylinder mould；The interior cylinder mould is cylinder made of stainless steel material
Type cylinder mould, the interior a diameter of 8cm of cylinder mould, the interior a height of 15cm of cylinder mould, interior cylinder mould aperture is 0.5mm, 1mm, 2mm or 5mm, in interior cylinder mould
Outside be also equipped with a lid for being capable of free-open-close.
As an optimization, the specification of the organic glass seal box is long 80cm, wide 60cm, high 60cm.
As an optimization, the collection system includes support plate, collecting net, collects rack and supporting rack；Supporting rack is round platform
Type stainless steel frame, supporting rack base diameter 30cm, supporting rack top diameter 10cm, high 30cm, supporting rack inner upper end has oblique
Downward stainless steel bracing plate, support plate play the role of cylinder mould in support when collecting sample；It is provided with supporting rack stainless
Steel collects rack, and collection rack is cylindrical type, and collecting net, which is freely placed at, to be collected on rack, and the aperture of collecting net is 0.2mm.
The present invention also provides the application method of the experimental provision of the micro- plastics ecological niche on-spot study of above-mentioned seawater, it include with
Lower step：
(1) the interior cylinder mould of bearing system is opened, micro- plastics of the different-grain diameter to be studied and material are launched respectively
In interior cylinder mould, the lid of interior cylinder mould is then shut, the net door of the outer cylinder mould of rectangle is opened, cylinder mould in cylinder is launched to rectangle outer net
In cage, outer cylinder mould net door is closed；
(2) bearing system and ballasting system are fixed near Sea Area Survey erect-position using anchor, anchor chain and rope；
(3) inlet valve of ballasting system is opened, outlet valve is closed, sea is poured into the organic glass seal box of ballasting system
Water, immerses seawater underwater 0.4-0.6m by bearing system under the pressure effect of ballasting system, stops sealing to organic glass
Case is poured water, and closes inlet valve, starts the experiment in situ of micro- plastics Study on Niche；
(4) the design sample time is needed according to experiment, when collecting sample, presses the electromagnetic switch of connection buoy, electricity first
Magnetic signal is input on solenoid valve by the control panel in ballasting system, after solenoid valve receives signal, opens gas cylinder, oxygen cylinder
Oxygen is discharged, increases the buoyancy of ballasting system, bearing system is emerged；Then, the net door of outer cylinder mould side is opened, is taken out
Interior cylinder mould；Shut outer cylinder mould net behind the door, open inlet valve and continue to sink under the water bearing system 0.4-0.6m, then shut into water
Valve, continues experiment in situ；After repeatedly, organic glass seal box is full of by seawater and oxygen, need to open outlet valve
Seawater in organic glass seal box and gas are discharged, oxygen cylinder is replaced, is then shut off outlet valve, then reopen into water
Valve, bearing system is pressed under water, continues subsequent experimental；
(5) lid of cylinder mould, collects micro- plastics in opening；First, collecting net is routed to and collected on rack, pressed from both sides with clip
Firmly, the lid of cylinder mould side in then opening, in the support plate for the stainless steel bracing frame for being placed on collection system, with mistake in advance
The clean sea water filtered, in flushing on the outside of cylinder mould, under flow action, micro- plastics in interior cylinder mould are washed into rapidly collecting net
In, then collecting net net bottom is cut, the micro- plastics that will accumulate in collecting net net bottom are put into togerther jelly together with the collecting net cut
Deposit pipe, after good seal, mark is finished writing on cryopreservation tube, be put into that liquid nitrogen container is quick-frozen rapidly, micro- plastic sample of collection is taken back into reality
Test room and carry out biological analysis；
(6) while collecting sample, the water quality characteristic that micro- plastics launch area, including temperature, dissolved oxygen, pH, salinity are monitored,
And collect certain volume water sample and take back laboratory progress nutrient concentrations analysis, by surveyed water quality index and the micro- plastics of different type
In biological characteristic analysis result be combined, carry out the response point of micro- plastics ecological niche architectural feature and culture zone envirment factor
Analysis, calculates the correlation of micro- plastic type and envirment factor and micro- plastics ecological niche architectural feature, determines to influence its structure spy
The leading factor of sign.
The micro- plastics ecological niche on-spot study experimental provision of seawater of the present invention and its application method, pass through and build and nature seawater
The consistent experiment condition of the environmental parameter such as water Inversion phenomenon, pH and nutritive salt, utilizes anchoring system, bearing system and ballasting system
Different types of micro- plastics are placed in marine environment, carry out experiment in situ, ensure that determining for research object and study condition
Property, the uniformity of experimental period, overcome field investigation collect micro- plastic material, shapes and sizes it is uncertain and complicated,
And the shortcomings that being difficult to reduce true marine environment under laboratory condition.Meanwhile the ballasting system in the device realizes carrying
The automatic of system floats and sinks, and facilitates the dispensing and collection of sample.In addition, interior cylinder mould is firmly supported at branch by collection system
On support, the shortcomings that operational coordination is bad on ship is overcome, realizes the quick collection of micro- plastic sample, this device in situ
Application to analyze micro- plastics niche characteristics, and its provide important leverage with envirment factor correlation.
Brief description of the drawings
Below by embodiment combination attached drawing to the experimental provision of the micro- plastics ecological niche on-spot study of seawater of the present invention and its
Application method is described further：
Fig. 1 is the structure diagram of anchoring system of the invention, bearing system and ballasting system：1- anchors, 2- anchor chains, 3- ropes
The outer cage of rope, 4-, cage in 5,6 ballast tanks, oxygen cylinder 8- solenoid valves, the first electric wires of 9-, 10- control panels, the electricity of 11- second built in 7-
Line, 12- electromagnetic switch, 13- buoys, 14- inlet valves and 15 drain valves.
Fig. 2 is the collection system structure diagram in experimental provision of the present invention：16- support plates, 17- collecting nets, 18- are collected
Rack, 19- supporting racks.
Embodiment
Embodiment 1
A kind of experimental provision of the micro- plastics ecological niche on-spot study of seawater, as shown in Figure 1, it includes anchoring system, carrying
System, ballasting system and collection system；Anchoring system connects bearing system, and ballasting system is fixedly connected on above bearing system；
Collection system is used to collect the sample obtained；
The anchoring system includes anchor 1, anchor chain 2 and rope 3, and anchor chain both ends connect anchor and rope respectively, and rope connection is held
Loading system；
The bearing system includes outer cylinder mould and interior cylinder mould；Interior cylinder mould is placed in outer cylinder mould；The outer cylinder mould is one
80cm × 60cm × 60cm rectangle stainless steel cylinder moulds, outer cylinder mould aperture are 6cm, and being equipped with above the outer cylinder mould of rectangle being capable of free-open-close
Net door；The interior cylinder mould is cylindrical type cylinder mould made of stainless steel material, the interior a diameter of 8cm of cylinder mould, the interior a height of 15cm of cylinder mould,
Brushing anti-decaying paint, interior cylinder mould aperture are 0.5mm, can also be arranged to 1mm, 2mm or 5mm, are also equipped with the outside of interior cylinder mould
One lid that can be opened.
The ballasting system includes an organic glass seal box 6 being connected to above bearing system, organic glass sealing
The specification of case is long 80cm, wide 60cm, high 60cm；Organic glass seal box is equipped with inlet valve 14 and drain valve 15, organic glass
Oxygen cylinder 7, solenoid valve 8, the first electric wire 9, control panel 10, the second electric wire 11 and external connection buoy 13 are provided with glass seal box
Electromagnetic switch 12, solenoid valve is fixed at the gas outlet of oxygen cylinder, to control the opening and closing of oxygen cylinder gas outlet, the first electric wire 9
Connect solenoid valve 8 and control panel 10,11 one end of the second electric wire connection control panel 10, other end connection electromagnetic switch 12, the oxygen
Gas cylinder volume is 5 liters, opening or closing by electromagnetic switch, assists bearing system to float and sink.
The collection system is as shown in Fig. 2, it includes supporting rack, collecting net, collects rack and support plate；Supporting rack is circle
Bench-type, bottom and top are circular stainless steel coil, supporting rack base diameter 30cm, supporting rack top diameter 10cm, high 30cm,
Connected between upper and lower stainless steel coil by six roots of sensation stainless steel plate.The support plate approaches to be welded on supporting rack stainless steel plate side
The position of upper round platform, is used to support interior cylinder mould.Collection rack is provided with supporting rack, the collection rack is also by stainless steel
Texture into a Round Porous tubular structure (aperture 3cm), its top can accept collecting net, and aperture thereon can allow flushing
The seawater to get off flows out rapidly from collection device.The aperture of collecting net is 0.2mm.
The application method of the experimental provision of the micro- plastics ecological niche on-spot study of this seawater comprises the following steps：
(1) lid of cylinder mould in bearing system is first turned on, by the different-grain diameter to be studied and micro- plastics of material point
Do not launch in difference in cylinder mould, then shut the lid of interior cylinder mould, open the outer cylinder mould of rectangle, interior cylinder mould is launched to outside rectangle
In cylinder mould, outer cylinder mould net door is shut.
(2) bearing system and ballasting system are fixed to by Sea Area Survey erect-position (longitude using anchor, anchor chain and rope：122°
28.800 ', 37 ° 2.436 ' of latitude)) near.
(3) inlet valve of ballasting system is opened, seawater is poured into ballasting system, will be held under the pressure effect of ballasting system
Loading system immerses seawater underwater 0.5m or so, stops pouring water to ballasting system, closes ballasting system inlet valve, starts micro- plastics
The experiment in situ of Study on Niche.
(4) design sample time 7d, 14d, 28d, 56d, 112d is needed according to experiment, when collecting sample, the company of pressing first
The electromagnetic switch waterborne of buoy is connect, electromagnetic signal is input to by the control panel in ballasting system and is connected at oxygen cylinder gas outlet
Solenoid valve on, after solenoid valve receives signal, open gas cylinder, oxygen cylinder release oxygen, increase the buoyancy of ballasting system, will hold
Loading system emerges.Then, the net door of outer cylinder mould side is opened, cylinder mould in taking-up.After shutting outer cylinder mould, open inlet valve after
It is continuous that bearing system sinks under the water 0.5m, inlet valve is then shut, continues experiment in situ.After repeatedly, seawater and oxygen
The organic glass seal box of ballasting system is full of by gas, and the outlet valve opened on organic glass seal box arranges seawater and gas
Go out, replace oxygen cylinder, then reopen inlet valve again, bearing system is pressed under water, then proceeds by subsequent experimental.
(5) lid of cylinder mould, collects micro- plastics in opening.First, collecting net is routed to and collected on rack, pressed from both sides with clip
Firmly, the lid of cylinder mould in then opening, in the support plate for the stainless steel bracing frame for being placed on collection system, with filtering in advance
Clean sea water, in flushing on the outside of cylinder mould, under flow action, micro- plastics of interior cylinder mould, which are washed into rapidly, is built in supporting rack
Collecting net in, then the net bottom of collecting net is cut, bag wherein and is gathered in micro- plastics of net bottom together with the net cut
It is put into togerther cryopreservation tube, after good seal, mark is finished writing on cryopreservation tube, be put into that liquid nitrogen container is quick-frozen rapidly, by micro- plastics of collection
Sample takes back laboratory and carries out biotinylated biomolecule signature analysis.
(6) while collecting sample, the water quality characteristic that micro- plastics launch area, including temperature, dissolved oxygen, pH, salinity are monitored,
And collect certain volume water sample and take back laboratory progress nutrient concentrations (inorganic nitrogen and phosphate) analysis, by surveyed water quality index
Be combined with the biological characteristic analysis result in the micro- plastics of different type, carry out micro- plastics niche characteristics and culture zone environment because
The response analysis of son, calculates micro- plastic type and envirment factor and micro- plastics ecological niche architectural feature (diversity and richness
Deng) correlation, determine to influence the leading factor of micro- plastics ecological niche architectural feature.
Claims (5)
1. a kind of experimental provision of the micro- plastics ecological niche on-spot study of seawater, it is characterised in that it includes anchoring system, carrying system
System, ballasting system and collection system；Anchoring system connects bearing system, and ballasting system is connected to above bearing system；Collect system
System is used to collect the sample obtained；
The anchoring system includes anchor, anchor chain and rope, and anchor chain both ends connect anchor and rope, rope connection bearing system respectively；
The bearing system includes outer cylinder mould and interior cylinder mould；Interior cylinder mould is placed in outer cylinder mould；
The ballasting system includes an organic glass seal box being connected to above bearing system, is set on organic glass seal box
There are inlet valve and drain valve, oxygen cylinder, solenoid valve, the first electric wire, control panel and the second electricity are provided with organic glass seal box
Line, organic glass seal box are provided outside the electromagnetic switch of connection buoy, and solenoid valve is fixed at the gas outlet of oxygen cylinder, by electricity
The opening and closing of magnetic switch control oxygen cylinder gas outlet, the first electric wire connection solenoid valve and control panel, the connection control of second electric wire one end
Plate, other end connection electromagnetic switch, opening or closing by electromagnetic switch, assists bearing system to float or sink.
A kind of 2. experimental provision of the micro- plastics ecological niche on-spot study of seawater according to claim 1, it is characterised in that institute
It is a 80cm × 60cm × 60cm rectangle stainless steel cylinder moulds to state outer cylinder mould, and outer cylinder mould aperture is 6cm, is set above the outer cylinder mould of rectangle
There is the veil door for being capable of free-open-close；The interior cylinder mould is cylindrical type cylinder mould made of stainless steel material, and interior cylinder mould is a diameter of
8cm, the interior a height of 15cm of cylinder mould, interior cylinder mould aperture is 0.5mm, 1mm, 2mm or 5mm, and one is also equipped with the outside of interior cylinder mould
It is capable of the lid of free-open-close.
A kind of 3. experimental provision of the micro- plastics ecological niche on-spot study of seawater according to claim 1, it is characterised in that institute
The specification for stating organic glass seal box is long 80cm, wide 60cm, high 60cm.
A kind of 4. experimental provision of the micro- plastics ecological niche on-spot study of seawater according to claim 1, it is characterised in that institute
Stating collection system includes support plate, collecting net, collects rack and supporting rack；Supporting rack is circular platform type stainless steel frame, supporting rack bottom
Portion diameter 30cm, supporting rack top diameter 10cm, high 30cm, supporting rack inner upper end have stainless steel bracing plate obliquely,
Support plate plays the role of cylinder mould in support when collecting sample；Stainless steel is provided with supporting rack and collects rack, collects rack
For cylindrical type, collecting net, which is freely placed at, to be collected on rack, and the aperture of collecting net is 0.2mm.
5. the application method of the experimental provision of the micro- plastics ecological niche on-spot study of seawater described in claim 1, it is characterised in that it
Comprise the following steps：
(1) the interior cylinder mould of bearing system is opened, micro- plastics of the different-grain diameter to be studied and material are launched into Intranet respectively
In cage, the lid of interior cylinder mould is then shut, the net door of the outer cylinder mould of rectangle is opened, cylinder mould in cylinder is launched to cylinder mould outside rectangle
In, close outer cylinder mould net door；
(2) bearing system and ballasting system are fixed near Sea Area Survey erect-position using anchor, anchor chain and rope；
(3) inlet valve of ballasting system is opened, outlet valve is closed, seawater is poured into the organic glass seal box of ballasting system,
Bearing system is immersed into seawater underwater 0.4-0.6m under the pressure effect of ballasting system, stops filling to organic glass seal box
Water, closes inlet valve, starts the experiment in situ of micro- plastics Study on Niche；
(4) the design sample time is needed according to experiment, when collecting sample, presses the electromagnetic switch of connection buoy, electromagnetism letter first
Number it is input to by the control panel in ballasting system on solenoid valve, after solenoid valve receives signal, opens gas cylinder, oxygen cylinder release
Oxygen, increases the buoyancy of ballasting system, bearing system is emerged；Then, the net door of outer cylinder mould side is opened, takes out Intranet
Cage；Shut outer cylinder mould net behind the door, open inlet valve and continue to sink under the water bearing system 0.4-0.6m, then shut inlet valve,
Continue experiment in situ；After repeatedly, organic glass seal box is full of by seawater and oxygen, and need to open outlet valve will have
Seawater and gas discharge in machine glass capsulation case, replace oxygen cylinder, are then shut off outlet valve, then reopen inlet valve, will
Bearing system press-in is underwater, continues subsequent experimental；
(5) lid of cylinder mould, collects micro- plastics in opening；First, collecting net is routed to and collected on rack, clamped with clip,
Then the lid of cylinder mould side in opening, in the support plate for the stainless steel bracing frame for being placed on collection system, with filtering in advance
Good clean sea water, in flushing on the outside of cylinder mould, under flow action, micro- plastics in interior cylinder mould are washed into rapidly collecting net
In, then collecting net net bottom is cut, the micro- plastics that will accumulate in collecting net net bottom are put into togerther jelly together with the collecting net cut
Deposit pipe, after good seal, mark is finished writing on cryopreservation tube, be put into that liquid nitrogen container is quick-frozen rapidly, micro- plastic sample of collection is taken back into reality
Test room and carry out biological analysis；
(6) while collecting sample, the water quality characteristic that micro- plastics launch area, including temperature, dissolved oxygen, pH, salinity are monitored, and receive
Collection certain volume water sample takes back laboratory and carries out nutrient concentrations analysis, by surveyed water quality index and the micro- plastics of different type
Biological characteristic analysis result is combined, and carries out the response analysis of micro- plastics ecological niche architectural feature and culture zone envirment factor, meter
The correlation of micro- plastic type and envirment factor and micro- plastics ecological niche architectural feature is calculated, determines to influence the master of its architectural feature
Inducement element.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711147898.5A CN107957490B (en) | 2017-11-17 | 2017-11-17 | Experimental device for seawater micro-plastic ecological niche in-situ research and using method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711147898.5A CN107957490B (en) | 2017-11-17 | 2017-11-17 | Experimental device for seawater micro-plastic ecological niche in-situ research and using method |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN107957490A true CN107957490A (en) | 2018-04-24 |
| CN107957490B CN107957490B (en) | 2020-04-03 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711147898.5A Active CN107957490B (en) | 2017-11-17 | 2017-11-17 | Experimental device for seawater micro-plastic ecological niche in-situ research and using method |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN109238914A (en) * | 2018-11-23 | 2019-01-18 | 中国科学院南京地理与湖泊研究所 | The research device and analysis method that different-grain diameter biology influences organic matter degradation |
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| CN115468882A (en) * | 2022-09-15 | 2022-12-13 | 中国水利水电科学研究院 | Indoor simulation device for measuring sedimentation rate of microbial plastic biofilm in water body |
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