CN107950775A - A kind of pig interferon derivant and its preparation method and application - Google Patents
A kind of pig interferon derivant and its preparation method and application Download PDFInfo
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- CN107950775A CN107950775A CN201711497883.1A CN201711497883A CN107950775A CN 107950775 A CN107950775 A CN 107950775A CN 201711497883 A CN201711497883 A CN 201711497883A CN 107950775 A CN107950775 A CN 107950775A
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/105—Aliphatic or alicyclic compounds
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/116—Heterocyclic compounds
- A23K20/121—Heterocyclic compounds containing oxygen or sulfur as hetero atom
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/142—Amino acids; Derivatives thereof
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/30—Feeding-stuffs specially adapted for particular animals for swines
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/60—Feeding-stuffs specially adapted for particular animals for weanlings
Abstract
The present invention discloses a kind of pig interferon derivant and its preparation method and application, and the pig interferon derivant includes the component of following mass fraction：101~251 parts of 50~200 parts of N acetylcysteines, 50~100 parts of tributyrin, 1 part of Puerarin and carrier.Pig interferon derivant provided by the invention, the derivant produced using N acetylcysteines, tributyrin and Puerarin as interferon in induction piglet body, it is directly appended to feed piglet in feed in actual use, interferon expression in piglet body is can induce, and strengthens the anti-virus ability of piglet；Meanwhile also have the advantages that without any side effects to piglet, safe to use.
Description
Technical field
The present invention relates to additive for feed for piglets technical field, more particularly to a kind of pig interferon derivant and its preparation side
Method and purposes.
Background technology
Interferon (Interferon, IFN) is produced by a variety of derivants (virus, bacterium and macromolecular etc.) inducing cell
There is antiviral, antitumor and immunoloregulation function class protein, the discovery of interferon is to animal viral disease
Treatment provides a kind of new method.But the comparision of production of exogenous interferon is difficult, low output, price costly, even
The interferon biological activity produced by transgenic technology is also than relatively low.In addition, research confirms that exogenous interferon has certain
Toxicity, can cause the adverse effects such as animal Neuroleptic Leukocytopenia, dysfunction of liver, infringement nervous system, be caused sternly to animal productiong
Lose again.And use and be apt to raw endogenous interferon in interferon inducer induced animal body, then it can overcome using exogenous dry
Disturb the detrimental effect of element.
At present, use of the vaccine for interferon inducer in cultivation practice is commonplace, but there are poultry feeders work
The problems such as amount is big, vaccine is infected just for specific virus.Also studies have pointed out that Chinese herbal medicine is also good interferon inducer,
But medicinal herb components are complicated, extraction process imperfection, are difficult to standardize, and still have problems to solve as interferon inducer
Certainly.
The content of the invention
The main object of the present invention is to propose a kind of pig interferon derivant and its preparation method and application, it is intended to uses pig
After interferon inducer feeding piglet, you can induce interferon expression in piglet body and strengthen piglet disease-resistant poison ability, using just
It is prompt.
To achieve the above object, the present invention proposes a kind of pig interferon derivant, includes the component of following mass fraction：
50~200 parts of N-acetylcystein, 50~100 parts of tributyrin, 1 part of Puerarin and carrier 101~
251 parts.
Preferably, the carrier includes any one in defatted rice bran, silica, zeolite powder and wheat-middlings.
The present invention also proposes a kind of preparation method of pig interferon derivant as described above, comprises the following steps：
Tributyrin is mixed with carrier, forms mixture；
Mixture is mixed again with N-acetylcystein and Puerarin, that is, obtains pig interferon derivant.
The present invention is it is further proposed that the purposes of pig interferon derivant as described above, by the pig interferon derivant
It is added in feed, is fed with piglet.
Preferably, additive amount of the pig interferon derivant in feed is the 0.05~0.15% of feeding quality.
Pig interferon derivant provided by the invention, using N-acetylcystein, tributyrin and Puerarin to lure
The derivant that interferon produces in piglet body is led, is directly appended to feed piglet in feed in actual use, you can induction piglet
Internal interferon expression, and strengthen the anti-virus ability of piglet；Meanwhile also have it is without any side effects to piglet, use peace
The advantages of complete.
Embodiment
, below will be in the embodiment of the present invention to make the purpose, technical scheme and advantage of the embodiment of the present invention clearer
Technical solution be clearly and completely described.The person that is not specified actual conditions in embodiment, builds according to normal condition or manufacturer
The condition of view carries out.Reagents or instruments used without specified manufacturer, is the conventional production that can be obtained by commercially available purchase
Product.
The present invention proposes a kind of pig interferon derivant and preparation method thereof, in pig interferon derivant provided by the invention
An embodiment in, the pig interferon derivant includes the component of following mass fraction：N-acetylcystein 50~200
Part, 50~100 parts of tributyrin, 1 part of Puerarin and 101~251 parts of carrier.Wherein, N-acetylcystein and Pueraria lobota
Root element is in solid-like, and tributyrin is oily liquids, it is necessary to by means of the carrier (carrier is solid-state), by described
Carrier will tributyrin carry out uniform adsorption after form solid mixt, then by secondary solid mixt and N- mucolyticums
Acid and Puerarin are uniformly mixed, and then the pig interferon derivant is made.
Pig interferon derivant provided in this embodiment, using functional nutrient material as derivant, such as half Guang of N- acetyl
Propylhomoserin and tributyrin generate cysteine and butyric acid respectively after gut metabolism, are the nutriments of animal body, right
Animal body is without any side effects, can use safely；And can be directly appended to be used to feed piglet in feed, play induction
Interferon expression and strengthen the effect of the anti-virus ability of piglet in piglet body, overcome existing vaccine just for specific virus sense
It is the shortcomings that dye, easy to use, reduce the workload of poultry feeders.
Wherein, N-acetylcystein can be effectively improved the function of intestinal canal of animal, strengthen as a kind of functional amino
The immunity of animal.Tributyrin is also a kind of functional materials, it is slowly discharged into fourth under the action of pancreatic lipase
Acid and glycerine, have the function that to repair intestinal villi, suppress harmful intestinal tract bacteria, promote the absorption of nutriment and utilize, by three
Glycerol monobutyralte uses on young animal, also has the diarrhea after reducing young animal wean, reduces weaning stress, is added to
The effect of motility rate and daily gain.And Puerarin is monomer isoflavone compounds, having improves ischemic myocardium circulation, suppresses blood platelet
Aggregation, reduces blood viscosity, improves the effect of microcirculation, is usually used in treating vascular diseases in physianthropy, the present invention is implemented
In example, Puerarin and N-acetylcystein, tributyrin collocation are used, pig interferon derivant is used to prepare, makes
The pig interferon derivant is obtained to induce in piglet body by the effect of N-acetylcystein and tributyrin
Interferon expression, strengthens piglet disease-resistant poison ability, moreover it is possible to improves the blood circulation of piglet, is more conducive to the healthy aquaculture of piglet.
In the technical solution of the present invention, N-acetylcystein, tributyrin and Puerarin can be directly appended to
Piglet is fed in feed (but since tributyrin is oily liquids, directly can not equably mix very much with solid feed
Close), itself and carrier can also be mixed and made into pre-mixing agent, or premix or concentrate feed shape are mixed and made into feed addictive
Formula, is then added in feed and feeds piglet.In the present embodiment, using by N-acetylcystein, tributyrin and Pueraria lobota
Root element is mixed and made into the form of pre-mixing agent with carrier, is then added in feed.
Wherein, the carrier includes any one in defatted rice bran, silica, zeolite powder and wheat-middlings.Passing through will
Pre-mixing agent form is made after carrier mixing in N-acetylcystein, tributyrin and Puerarin, is conducive to improve N- second
Acyl cysteine, tributyrin and Puerarin mixing uniformity, and N-acetylcystein, tributyrin and
Dispersiveness after Puerarin mixing in feed.In specific embodiment provided by the invention, using the carrier as defatted rice bran
Illustrated exemplified by (defatted rice bran can be used to feed in itself).
In the embodiment that the preparation method of pig interferon derivant is proposed in the present invention, the pig interferon derivant
Preparation method comprises the following steps：
Tributyrin is mixed with carrier, forms mixture；
Mixture is mixed again with N-acetylcystein and Puerarin, that is, obtains pig interferon derivant.
Tributyrin and carrier are sufficiently mixed according to predetermined ratio, by the carrier by tributyrin into
Solid mixt is formed after row uniform adsorption, then the solid mixt is filled with N-acetylcystein and Puerarin again
Divide mixing, form the pig interferon derivant after mixing, preparation method is simple and practicable.Wherein, the mode of mixing can
To be hand mix or be mixed by common batch mixing apparatus, as long as raw material is sufficiently mixed uniformly.
The present invention, will the pig interferon induction it is further proposed that the purposes of pig interferon derivant as described above
Agent is added in feed, is fed with piglet.Pig interferon derivant provided by the invention is added to after feeding piglet in feed, can
Induce interferon expression in piglet body and strengthen piglet disease-resistant poison ability, and then improve the growth performance of piglet.
Wherein, additive amount of the pig interferon derivant provided by the invention in feed for feeding quality 0.05~
0.15% (containing the pig interferon derivant 0.025~0.075% in actual feed).In specific implementation provided by the invention
In example, illustrated using additive amount of the pig interferon derivant in feed as the 0.15% of feeding quality.
Technical scheme is described in further detail below in conjunction with specific embodiment, it will be appreciated that following real
Example is applied only to explain the present invention, is not intended to limit the present invention.
Embodiment 1
By tributyrin and defatted rice bran by weight 50：101 are thoroughly mixed to form mixture, then by mixture
With N-acetylcystein and Puerarin by weight 151：50：1 is sufficiently mixed, and obtains pig interferon derivant.
Embodiment 2
By tributyrin and defatted rice bran by weight 100：151 are thoroughly mixed to form mixture, then will mixing
Thing is with N-acetylcystein and Puerarin by weight 251：50：1 is sufficiently mixed, and obtains pig interferon derivant.
Embodiment 3
By tributyrin and defatted rice bran by weight 200：251 are thoroughly mixed to form mixture, then will mixing
Thing is with N-acetylcystein and Puerarin by weight 451：50：1 is sufficiently mixed, and obtains pig interferon derivant.
Embodiment 4
By tributyrin and defatted rice bran by weight 50：101 are thoroughly mixed to form mixture, then by mixture
With N-acetylcystein and Puerarin by weight 151：100：1 is sufficiently mixed, and obtains pig interferon derivant.
Embodiment 5
By tributyrin and defatted rice bran by weight 100：151 are thoroughly mixed to form mixture, then will mixing
Thing is with N-acetylcystein and Puerarin by weight 251：100：1 is sufficiently mixed, and obtains pig interferon derivant.
Embodiment 6
By tributyrin and defatted rice bran by weight 200：251 are thoroughly mixed to form mixture, then will mixing
Thing is with N-acetylcystein and Puerarin by weight 451：100：1 is sufficiently mixed, and obtains pig interferon derivant.
Application Example
The weight disposably to wean of the 168 first 21 ages in days weanling pig consistent with gender is chosen, as experiment piglet, and
7 groups are randomly divided into, every group 3 nose heave multiple (i.e. every group of 3 column of repetition), per 8, column, is fed with basal diet.Wherein, 7 groups of examinations
Test piglet and be followed successively by I group, II group, III group, IV group, V group, VI group and VII group.I group is control group, and matter is added in basal diet
Measure the colistin sulfate pre-mixing agent that fraction is 0.04%；II group, III group, IV group, V group, VI group and VII group is test group, successively
Pig interference prepared by embodiment 1, embodiment 2, embodiment 3, embodiment 4, embodiment 5 and embodiment 6 is added in basal diet
Plain derivant, additive amount are the 0.15% of feeding quality.
After feeding 21 days, the growth performance of statistical test piglet, and gather blood sample and intestinal tissue sample is divided
Analysis test, the blood interferon-' alpha ' (IFN-α) of testing experiment piglet blood sample is horizontal respectively, and the sky of intestinal tissue sample
Intestines interferon and its antiviral signal path key molecule gene mRNA levels, specific test method and test result are as follows：
(1) piglet growth performance
The changes of weight before and after experiment piglets is weighed, calculates its average daily gain, while calculates each group experiment piglet
Feed feed-weight ratio；Diarrhea standard is separated into liquid, shapeless, liquid dung to test piglet excrement, it is every in units of group
Its record diarrhea head number statistics diarrhea rate, diarrhea rate calculation formula are：Every group of total diarrhea head of diarrhea rate (%)=in experimental period
Number/(every group of piglet head number × experiment number of days) × 100%；The statistical result of average daily gain, feed-weight ratio and diarrhea rate such as table 1
It is shown.It should be noted that in all tables of the present embodiment, colleague's shoulder mark difference lowercase letter indication difference significantly (P<
0.05)。
The growth performance statistical result of each group experiment piglet in 1 Application Example of table
From the statistical result of table 1, piglet interferon inducer prepared by the embodiment of the present invention is added in feed
Feed piglet, with control group (I group, addition mass fraction be 0.04% colistin sulfate pre-mixing agent) compare, II group, III group,
IV group, V group, VI group and VII group be respectively increased piglet average daily gain 10.64%, 16.22%, 13.56%, 1.60%,
6.12%th, 7.18% (P ＜ 0.05), reduces feed-weight ratio 8.99%, 14.04%, 11.24%, 1.69%, 5.06%, 6.18%
(P ＜ 0.05), significantly reduces 37.13%, 36.53%, 46.11%, 30.54%, 14.97%, 17.96% (P ＜ of diarrhea rate
0.05).Illustrate, using pig interferon derivant provided in an embodiment of the present invention, the growth performance of piglet can be obviously improved, dropped
The diarrhea rate of low piglet.
(2) blood interferon-' alpha ' is horizontal
In a manner of vena cava anterior, the blood of I group, II group, III group, IV group, V group, VI group and VII group experiment piglet is gathered respectively
Liquid sample, IFN-α kit (the Interferon beta Pig of Abcam companies are quantitatively detected using solid phase sandwich ELISA
ELISA Kit), the concentration level (application method that test method is provided with reference to kit) of IFN-α in blood sample is measured, is surveyed
Determine shown in result following 2.
The horizontal measurement result of blood interferon-' alpha ' of each group experiment piglet in 2 Application Example of table
From the measurement result of table 2, piglet interferon inducer prepared by the embodiment of the present invention is added in feed
Piglet is fed, compared with control group, II group, III group, IV group, V group, VI group and VII group is respectively increased piglet blood IFN-α concentration
Level 140%, 165%, 98.26%, 15.65%, 41.74%, 78.26% (P ＜ 0.05).Illustrate, implement using the present invention
The pig interferon derivant that example provides, can play significant inducing action, and strengthen piglet to the secretion of interferon-' alpha ' in piglet body
The viral infection ability of resistance.
(3) jejunum interferon and its antiviral signal path key molecule gene mRNA levels
By quantitative real-time PCR, (result carries out statistical analysis with 2- Δ Δ Ct methods, and 1) control group is set to, measure
The jejunum interferon of intestine of young pigs tissue sample and its antiviral signal path key molecule gene mRNA levels are tested, wherein,
Jejunum interferon includes interferon-' alpha ' (IFN-α), interferon-beta (IFN-β), interferon-γ (IFN-γ), 1 (IL- of interferon-λ
29) and interferon-λ 3 (IL-28B), jejunum interferon anti-reflecting virus signal path key molecule gene mRNA levels include interferon
Stimulated gene (ISG15), the interferon-induced Membrane Protein 1 (IFITM1), interferon-induced transferring film albumen 3 (IFITM3), disease-resistant of turning
The gene mRNA levels of toxalbumin MX1, antiviral protein MX2 and oligoadenylate synthetase (OAS1), the measurement result such as institute of table 3
Show.
Each group experiment Jejunum of Piglets interferon and its antiviral signal path key molecule gene in 3 Application Example of table
MRNA level in-site measurement result
From the measurement result of table 3, piglet interferon inducer prepared by the embodiment of the present invention is added in feed
Feed piglet, compared with control group, II group, III group, IV group, V group, VI group and VII group can improve intestine of young pigs interferon-' alpha ',
The gene expression of β, λ, show that pig interferon derivant provided in an embodiment of the present invention can promote the gene of intestine of young pigs interferon
Transcription, promotes interferon generation.In addition, compared with control group, II group, III group, IV group, V group, VI group and VII group also improves
ISG15, IFITM1 and IFITM3, the interferon-induced isogenic mRNA level in-site of antiviral protein MX1, MX2 and OAS1, explanation
Pig interferon derivant provided in an embodiment of the present invention improves the conjunction of enteron aisle antiviral protein by promoting the expression of interferon
Into, and then the anti-virus ability of piglet is enhanced, ensure intestine of young pigs health.
In conclusion pig interferon derivant provided in this embodiment, at least has the following advantages：(1) sought with feature
Support material and be used as derivant, as N-acetylcystein and tributyrin generated respectively after gut metabolism cysteine with
Butyric acid, is the nutriment of animal body, without any side effects to animal body, can be used safely；(2) pig interferon lures
Agent is led in practical application, can be directly appended to be used to feed piglet in feed, you can play interferon in induction piglet body
Express and strengthen the effect of the anti-virus ability of piglet, overcome the shortcomings that existing vaccine is infected just for specific virus, use
It is convenient, reduce the workload of poultry feeders, there is huge application potential.
It these are only the preferred embodiment of the present invention, be not intended to limit the scope of the invention, for this area
For technical staff, the invention may be variously modified and varied.It is all in the spirit and principles in the present invention etc, that is made is any
Modification, equivalent substitution, improvement etc., should all be included within the scope of the present invention.
Claims (5)
1. a kind of pig interferon derivant, it is characterised in that include the component of following mass fraction：
50~200 parts of N-acetylcystein, 50~100 parts of tributyrin, 1 part of Puerarin and carrier 101~251
Part.
2. pig interferon derivant as claimed in claim 1, it is characterised in that the carrier includes defatted rice bran, titanium dioxide
Any one in silicon, zeolite powder and wheat-middlings.
A kind of 3. preparation method of pig interferon derivant as described in 1 or 2 any one of claim, it is characterised in that bag
Include following steps：
Tributyrin is mixed with carrier, forms mixture；
Mixture is mixed again with N-acetylcystein and Puerarin, that is, obtains pig interferon derivant.
4. the purposes of pig interferon derivant as claimed in claim 1 or 2, it is characterised in that induce the pig interferon
Agent is added in feed, is fed with piglet.
5. the purposes of pig interferon derivant as claimed in claim 4, it is characterised in that the pig interferon derivant is being raised
Additive amount in material is the 0.05~0.15% of feeding quality.
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