CN107950752A - A kind of lotus leaf feed for improving laying rate - Google Patents

A kind of lotus leaf feed for improving laying rate Download PDF

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Publication number
CN107950752A
CN107950752A CN201711427024.5A CN201711427024A CN107950752A CN 107950752 A CN107950752 A CN 107950752A CN 201711427024 A CN201711427024 A CN 201711427024A CN 107950752 A CN107950752 A CN 107950752A
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China
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parts
lotus leaf
compound additive
laying
feed
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CN201711427024.5A
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CN107950752B (en
Inventor
余劼
张著强
兰培坚
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FUJIAN GUANGYANG EGG INDUSTRY Co Ltd
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FUJIAN GUANGYANG EGG INDUSTRY Co Ltd
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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/14—Pretreatment of feeding-stuffs with enzymes
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K50/00—Feeding-stuffs specially adapted for particular animals
    • A23K50/70—Feeding-stuffs specially adapted for particular animals for birds
    • A23K50/75—Feeding-stuffs specially adapted for particular animals for birds for poultry

Abstract

The invention discloses a kind of lotus leaf feed for improving laying rate of laying hen, the raw materials by weight portion meter of the lotus leaf feed includes：50 80 parts of peanut meal, 20 30 parts of corn flour, 20 30 parts of soyabean expeller, 5 10 parts of mountain flour, 25 parts of fishbone dust, 13 parts of vegetable oil, 35 parts of compound additive, the compound additive are that lotus leaf, the fruit of glossy privet are made through submerged fermentation.Lotus leaf feed produced by the present invention can significantly improve the laying rate of laying hen and quality of laying eggs.

Description

A kind of lotus leaf feed for improving laying rate
Technical field
The invention belongs to egg feedstuff field, and in particular to a kind of lotus leaf feed for improving laying rate of laying hen.
Background technology
The growth of laying hen, laying eggs is required for certain nutriment, and the source of nutriment is mainly taken the photograph from feed Take.After laying hen obtains all kinds of nutriments, by internal digestion, metabolic activity, it is transformed into the body protein, amino acid, fat of chicken Fat, vitamin, glycogen etc., and then synthesize the chicken product of human demand.Feed addictive, refers in feed processing, storage, tune Match somebody with somebody and use during, to meet certain special requirement of animal, made an addition to using a variety of distinct methods some a small amount of in feed Or micro trophism or non-nutritive substance, be mixed feed important component.It, which has, improves efficiency of feed utilization, increases Add the palatability of daily ration, promote animal normal development and tachyauxesis, improve animal product quality, disease preventing and treating, easy to feed Storage and preservation, and improve the effect such as feed processing performance.Therefore, feed addictive is the core of mixed feed, its is each The amount and its match ratio and quality of component directly affect the efficiency of feed utilization and production performance of livestock and poultry fish, are the passes for developing aquaculture One of key technology, the feed addictive that research and development can improve laying rate of laying hen produce China's breeding layer chicken industry hair at a high speed Exhibition is of great significance.
The content of the invention
It is an object of the invention to provide a kind of lotus leaf feed for improving laying rate of laying hen, lotus leaf feed prepared by the present invention The laying rate of laying hen and quality of laying eggs can be significantly improved, extends the shelf life of feed.
To achieve the above object, the present invention adopts the following technical scheme that：
A kind of lotus leaf feed for improving laying rate of laying hen, the raw materials by weight portion meter of the lotus leaf feed include：Peanut meal 50- 80 parts, 20-30 parts of corn flour, 20-30 parts of soyabean expeller, 5-10 parts of mountain flour, 2-5 parts of fishbone dust, 1-3 parts of vegetable oil, compound addition 3-5 parts of agent；
The preparation method of the compound additive, includes the following steps：
（1）It is prepared by strain：Barms is inoculated on YPD slant mediums, inoculum concentration is the 0.1- of slant medium weight 0.3%, it is put at 26-28 DEG C and cultivates 5-8d；
（2）Triangular flask Shaking culture：Shaking speed 80-100r/min, stroke 5-6cm back and forth shake, 26-30 DEG C of shaken cultivation 2- 3d, is made seed suspension liquid；
（3）Seeding tank seed culture：By kind of amount 15-20%, 27-30 DEG C of a temperature, illumination condition：Continuous darkness condition, mixing speed 160-180r/min, filtrated air throughput are 2.5m per minute3, tank pressure 0.04MPa, fermented and cultured 48-72h；PH is down to 4.5-5.0；
（4）Fermentation tank Submerged fermentation：Inoculum concentration 25-30%, is inoculated in submerged fermentation culture medium, 27-30 DEG C of temperature, stirring Speed 50-80r/min, filtrated air throughput are 2m per minute3, tank pressure 0.05MPa, cultivates 72-80h, pH is down to 4.0- 4.5；
Submerged fermentation culture medium：By mass percentage, lotus leaf powder 50%, ligustrum lucidum powder 20%, glucose 5.0%, magnesium sulfate 0.3%th, dipotassium hydrogen phosphate 0.7%, sodium chloride 0.4%, camellia oil 0.3%, surplus are water, and pH=6-7,150 DEG C of sterilizing 15min, cool down It is spare afterwards；
（5）Enzymolysis：Proteinase-10 .5wt%, pectase 0.25wt%, fiber are added in submerged fermentation culture medium after fermented and cultured Plain enzyme 0.75wt%；Protease is 2 × 105U/kg, pectase are 3.0 × 105U/kg, cellulase are 1.5 × 105u/kg；Temperature 3-4h is digested at 55-60 DEG C of degree；
（6）Deacidification, homogeneous, low temperature drying obtain compound additive.
Preferably, the raw materials by weight portion meter of the lotus leaf feed includes：60 parts of peanut meal, 25 parts of corn flour, soya bean 25 parts of the dregs of rice, 8 parts of mountain flour, 3 parts of fishbone dust, 2 parts of vegetable oil, 4 parts of compound additive；
The remarkable advantage of the present invention is：
（One）In Fruits of Ligustrum Lucidum Ait, existing androgen material（Testosterone）Also there is estrogen substance（Estradiol）Presence, have property Hormone-like effect, can promote the development of reproduction cell, can also improve the relative weight of hen oviduct before sexal maturity, promote ovarian follicle Development, large follicle quantity showed increased, to achieve the purpose that to improve laying rate and extend the time of egg-laying peak.
（Two）Liver is the major organs of laying hen synthesis Yolk, its synthetic is most of with very low density lipoprotein (VLDL) form is transported to outside liver, is deposited to as yolk precursors matter in ovarian follicle.Under normal physiological condition, laying hen arrives Lay eggs the middle and later periods, fat constituent can gradually be incremented by with age in days in liver, and when accumulating to a certain extent, it is normal to influence liver cell Function, the physical capacity such as vitellin (VIG) declines necessary to making liver synthesis Yolk and follicle maturity, influences ovum Bubble is reached maturity.Thus when adipose-derived excessive in liver, the function of liver synthesis lipoprotein reduces and oxidation of fatty acids Reduced capability, phosphatide, which is reduced, then to be influenced lipoprotein formation and exports to influence to produce when factor causes fat metabolism to be affected Egg rate and Egg Quality.
Medicinal component in lotus leaf mainly has：（1）Alkaloid.Mainly include all kinds of Nuciferines, lotus stem alkali, nuciferine, cattle heart Fruit alkali, aporheine class, N- methyl coclaurines, anonaine, tulipiferine, armepavine etc.；（2）Flavonoids.Mainly include Quercetin, isoquercitrin, Hyperoside, astragalin, nelumboside, Kaempferol etc.；（3）Volatile organic matter.Share tens Kind, mainly there are alkane, alkene, alcohol, ether, aldehyde, ketone, ester, phenol etc.；（4）Organic acid, mainly including tartaric acid, citric acid, malic acid, Oxalic acid, butanedioic acid, grape acid, tannic acid etc..Lotus leaf mainly has following effect：（1）Lipid-loweringing.Active ingredient flavones in lotus leaf and Alkaloids substance can reduce the content of the T-CHOL, triglycerides, low density lipoprotein-cholesterol of laying hen etc., suppress Animal tallow is deposited on liver so that the function of liver cell is restored, improve fat metabolism so that play improve laying rate and Salubrious effect.（2）Oxidation resistant effect.Lotus Leafextract has good Scavenging ability, can effectively suppress Linoleic Acid Oxidation.（3）Bacteriostasis.Lotus Leafextract is to causing food spoilage and pathogenic bacteria to have significant fungistatic effect.
Embodiment
For the further open rather than limitation present invention, below in conjunction with example, the present invention is described in further detail.
Embodiment 1
A kind of lotus leaf feed for improving laying rate of laying hen, the raw materials by weight portion meter of the lotus leaf feed include：Peanut meal 50 Part, 20 parts of corn flour, 20 parts of soyabean expeller, 5 parts of mountain flour, 2 parts of fishbone dust, 1 part of vegetable oil, 3 parts of compound additive；
The preparation method of the compound additive, includes the following steps：
（1）It is prepared by strain：Barms is inoculated on YPD slant mediums, inoculum concentration is the 0.1% of slant medium weight, It is put at 26 DEG C and cultivates 5d；
（2）Triangular flask Shaking culture：Shaking speed 80r/min, stroke 5cm back and forth shake, and 26 DEG C of shaken cultivation 2d, are made seed Suspension；
（3）Seeding tank seed culture：By kind of amount 15%, 27 DEG C of a temperature, illumination condition：Continuous darkness condition, mixing speed 160r/ Min, filtrated air throughput are 2.5m per minute3, tank pressure 0.04MPa, fermented and cultured 48h；PH is down to 4.5；
（4）Fermentation tank Submerged fermentation：Inoculum concentration 25%, is inoculated in submerged fermentation culture medium, 27 DEG C of temperature, mixing speed 50r/min, filtrated air throughput are 2m per minute3, tank pressure 0.05MPa, cultivates 72h, pH is down to 4.0；
Submerged fermentation culture medium：By mass percentage, lotus leaf powder 50%, ligustrum lucidum powder 20%, glucose 5.0%, magnesium sulfate 0.3%th, dipotassium hydrogen phosphate 0.7%, sodium chloride 0.4%, camellia oil 0.3%, surplus are water, pH=6,150 DEG C of sterilizing 15min, after cooling It is spare；
（5）Enzymolysis：Add proteinase-10 .5wt%, pectase 0.25wt%, cellulase 0.75wt%；Protease is 2 × 105u/ Kg, pectase are 3.0 × 105U/kg, cellulase are 1.5 × 105u/kg；3h is digested at 55 DEG C of temperature；
（6）Deacidification, homogeneous, low temperature drying obtain compound additive.
Embodiment 2
A kind of lotus leaf feed for improving laying rate of laying hen, the raw materials by weight portion meter of the lotus leaf feed include：Peanut meal 60 Part, 25 parts of corn flour, 25 parts of soyabean expeller, 8 parts of mountain flour, 3 parts of fishbone dust, 2 parts of vegetable oil, 4 parts of compound additive；
The preparation method of the compound additive, includes the following steps：
（1）It is prepared by strain：Barms is inoculated on YPD slant mediums, inoculum concentration is the 0.2% of slant medium weight, It is put at 27 DEG C and cultivates 7d；
（2）Triangular flask Shaking culture：Shaking speed 90r/min, stroke 6cm back and forth shake, and 28 DEG C of shaken cultivation 2.5d, are made kind Sub- suspension；
（3）Seeding tank seed culture：By kind of amount 18%, 28 DEG C of a temperature, illumination condition：Continuous darkness condition, mixing speed 170r/ Min, filtrated air throughput are 2.5m per minute3, tank pressure 0.04MPa, fermented and cultured 60h；PH is down to 4.8；
（4）Fermentation tank Submerged fermentation：Inoculum concentration 28%, is inoculated in submerged fermentation culture medium, 28 DEG C of temperature, mixing speed 70r/min, filtrated air throughput are 2m per minute3, tank pressure 0.05MPa, cultivates 76h, pH is down to 4.2；
Submerged fermentation culture medium：By mass percentage, lotus leaf powder 50%, ligustrum lucidum powder 20%, glucose 5.0%, magnesium sulfate 0.3%th, dipotassium hydrogen phosphate 0.7%, sodium chloride 0.4%, camellia oil 0.3%, surplus are water, and pH=6.5,150 DEG C of sterilizing 15min, cool down It is spare afterwards；
（5）Enzymolysis：Add proteinase-10 .5wt%, pectase 0.25wt%, cellulase 0.75wt%；Protease is 2 × 105u/ Kg, pectase are 3.0 × 105U/kg, cellulase are 1.5 × 105u/kg；3h is digested at 58 DEG C of temperature；
（6）Deacidification, homogeneous, low temperature drying obtain compound additive.
Embodiment 3
A kind of lotus leaf feed for improving laying rate of laying hen, the raw materials by weight portion meter of the lotus leaf feed include：Peanut meal 80 Part, 30 parts of corn flour, 30 parts of soyabean expeller, 10 parts of mountain flour, 5 parts of fishbone dust, 3 parts of vegetable oil, 5 parts of compound additive；
The preparation method of the compound additive, includes the following steps：
（1）It is prepared by strain：Barms is inoculated on YPD slant mediums, inoculum concentration is the 0.3% of slant medium weight, It is put at 28 DEG C and cultivates 8d；
（2）Triangular flask Shaking culture：Shaking speed 100r/min, stroke 6cm back and forth shake, and 30 DEG C of shaken cultivation 3d, are made kind Sub- suspension；
（3）Seeding tank seed culture：By kind of amount 20%, 30 DEG C of a temperature, illumination condition：Continuous darkness condition, mixing speed 180r/ Min, filtrated air throughput are 2.5m per minute3, tank pressure 0.04MPa, fermented and cultured 72h；PH is down to 5.0；
（4）Fermentation tank Submerged fermentation：Inoculum concentration 30%, is inoculated in submerged fermentation culture medium, 30 DEG C of temperature, mixing speed 80r/min, filtrated air throughput are 2m per minute3, tank pressure 0.05MPa, cultivates 80h, pH is down to 4.5；
Submerged fermentation culture medium：By mass percentage, lotus leaf powder 50%, ligustrum lucidum powder 20%, glucose 5.0%, magnesium sulfate 0.3%th, dipotassium hydrogen phosphate 0.7%, sodium chloride 0.4%, camellia oil 0.3%, surplus are water, pH=7,150 DEG C of sterilizing 15min, after cooling It is spare；
（5）Enzymolysis：Add proteinase-10 .5wt%, pectase 0.25wt%, cellulase 0.75wt%；Protease is 2 × 105u/ Kg, pectase are 3.0 × 105U/kg, cellulase are 1.5 × 105u/kg；4h is digested under temperature 60 C；
（6）Deacidification, homogeneous, low temperature drying obtain compound additive.
Comparative example 1
Compound additive is not added in feed, remaining component and proportioning are the same as embodiment 1.
Comparative example 2
Compound additive is not added in feed, remaining component and proportioning are the same as embodiment 2.
Comparative example 3
Compound additive is not added in feed, remaining component and proportioning are the same as embodiment 3.
Layer Production Performance is tested
Experimental animal：- 500 age in days laying hen of 150 age in days is selected, kind is：Extra large blue brown laying hen；
Each 100 of the embodiment 1-3 laying hens identical with health status with the forage feed age in days of comparative example 1-3 is respectively adopted, often Day, feeding capacity was 120g/, and experiment laying hen is in same environment, identical conditions, unified feeding management.Kept in water fountain sufficient Drinking-water, freely drinks, and replace daily once.The situation of laying eggs of laying hen is counted, the results are shown in Table 1：
The average egg production and average egg weight for the laying hen that table 1 is fed using different feeds
As shown in Table 1, the laying rate of laying hen and quality of laying eggs can be significantly improved by adding the lotus leaf feed of compound additive, be extended Egg-laying peak.
2 compound additive main component of table and content（G/100g dry weights）
By in compound additive made from submerged fermentation, rich in materials such as general flavone, alkaloids, Nuciferines, as feed Additive, significantly improves the laying rate of laying hen and quality of laying eggs, extends egg-laying peak.
The foregoing is merely presently preferred embodiments of the present invention, all equivalent changes done according to scope of the present invention patent with Modification, should all belong to the covering scope of the present invention.

Claims (2)

  1. A kind of 1. lotus leaf feed for improving laying rate of laying hen, it is characterised in that the raw materials by weight portion meter of the lotus leaf feed Including：50-80 parts of peanut meal, 20-30 parts of corn flour, 20-30 parts of soyabean expeller, 5-10 parts of mountain flour, 2-5 parts of fishbone dust, vegetable oil 1-3 parts, 3-5 parts of compound additive；
    The preparation method of the compound additive, includes the following steps：
    （1）It is prepared by strain：Barms is inoculated on YPD slant mediums, inoculum concentration is the 0.1- of slant medium weight 0.3%, it is put at 26-28 DEG C and cultivates 5-8d；
    （2）Triangular flask Shaking culture：Shaking speed 80-100r/min, stroke 5-6cm back and forth shake, 26-30 DEG C of shaken cultivation 2- 3d, is made seed suspension liquid；
    （3）Seeding tank seed culture：By kind of amount 15-20%, 27-30 DEG C of a temperature, illumination condition：Continuous darkness condition, mixing speed 160-180r/min, filtrated air throughput are 2.5m per minute3, tank pressure 0.04MPa, fermented and cultured 48-72h；PH is down to 4.5-5.0；
    （4）Fermentation tank Submerged fermentation：Inoculum concentration 25-30%, is inoculated in submerged fermentation culture medium, 27-30 DEG C of temperature, stirring Speed 50-80r/min, filtrated air throughput are 2m per minute3, tank pressure 0.05MPa, cultivates 72-80h, pH is down to 4.0- 4.5；
    Submerged fermentation culture medium：By mass percentage, lotus leaf powder 50%, ligustrum lucidum powder 20%, glucose 5.0%, magnesium sulfate 0.3%th, dipotassium hydrogen phosphate 0.7%, sodium chloride 0.4%, camellia oil 0.3%, surplus are water, and pH=6-7,150 DEG C of sterilizing 15min, cool down It is spare afterwards；
    （5）Enzymolysis：Proteinase-10 .5wt%, pectase 0.25wt%, fiber are added in submerged fermentation culture medium after fermented and cultured Plain enzyme 0.75wt%；Protease is 2 × 105U/kg, pectase are 3.0 × 105U/kg, cellulase are 1.5 × 105u/kg；Temperature 3-4h is digested at 55-60 DEG C of degree；
    （6）Deacidification, homogeneous, low temperature drying obtain compound additive.
  2. A kind of 2. lotus leaf feed for improving laying rate of laying hen according to claim 1, it is characterised in that the lotus leaf feed Raw materials by weight portion meter include：60 parts of peanut meal, 25 parts of corn flour, 25 parts of soyabean expeller, 8 parts of mountain flour, 3 parts of fishbone dust, plant Thing oil 2 parts, 4 parts of compound additive.
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Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040044079A1 (en) * 2002-09-04 2004-03-04 The Iams Company Methods and compositions for weight control
CN103125779A (en) * 2013-03-22 2013-06-05 河北农业大学 Biological Chinese herbal feed additive for broiler chicken and preparation method thereof
CN104893992A (en) * 2015-06-30 2015-09-09 福建天斯顿生物科技有限公司 Submerged fermentation method for ganoderma lucidum
CN105010730A (en) * 2015-08-21 2015-11-04 何万领 Preparation method of probiotic fermented Chinese traditional herb feed additive for laying hens
CN107259194A (en) * 2017-07-31 2017-10-20 陈长辉 A kind of feed suitable for laying cycle of laying hens

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040044079A1 (en) * 2002-09-04 2004-03-04 The Iams Company Methods and compositions for weight control
CN103125779A (en) * 2013-03-22 2013-06-05 河北农业大学 Biological Chinese herbal feed additive for broiler chicken and preparation method thereof
CN104893992A (en) * 2015-06-30 2015-09-09 福建天斯顿生物科技有限公司 Submerged fermentation method for ganoderma lucidum
CN105010730A (en) * 2015-08-21 2015-11-04 何万领 Preparation method of probiotic fermented Chinese traditional herb feed additive for laying hens
CN107259194A (en) * 2017-07-31 2017-10-20 陈长辉 A kind of feed suitable for laying cycle of laying hens

Non-Patent Citations (1)

* Cited by examiner, † Cited by third party
Title
张玮，等: "女贞子粉对蛋鸡生产性能及免疫力的影响", 《中国家禽科学研究进展——第十四次全国家禽科学学术讨论会论文集》 *

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