CN107941661A - Classification reagent for hemocyte analyzers suit of one kind five and preparation method thereof - Google Patents
Classification reagent for hemocyte analyzers suit of one kind five and preparation method thereof Download PDFInfo
- Publication number
- CN107941661A CN107941661A CN201711177105.4A CN201711177105A CN107941661A CN 107941661 A CN107941661 A CN 107941661A CN 201711177105 A CN201711177105 A CN 201711177105A CN 107941661 A CN107941661 A CN 107941661A
- Authority
- CN
- China
- Prior art keywords
- reagent
- hemolytic agent
- agent
- suit
- hemocyte analyzers
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 239000003153 chemical reaction reagent Substances 0.000 title claims abstract description 32
- 210000003677 hemocyte Anatomy 0.000 title claims abstract description 21
- 229940000351 hemocyte Drugs 0.000 title claims abstract description 21
- 238000002360 preparation method Methods 0.000 title claims description 6
- 238000004043 dyeing Methods 0.000 claims abstract description 13
- 239000003219 hemolytic agent Substances 0.000 claims abstract description 13
- 238000010790 dilution Methods 0.000 claims abstract description 9
- 239000012895 dilution Substances 0.000 claims abstract description 9
- 230000002949 hemolytic effect Effects 0.000 claims description 34
- KDYFGRWQOYBRFD-UHFFFAOYSA-N succinic acid Chemical compound OC(=O)CCC(O)=O KDYFGRWQOYBRFD-UHFFFAOYSA-N 0.000 claims description 28
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 26
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 claims description 21
- LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical compound OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 claims description 20
- 239000004094 surface-active agent Substances 0.000 claims description 17
- 239000001384 succinic acid Substances 0.000 claims description 14
- 239000011780 sodium chloride Substances 0.000 claims description 13
- 229940051841 polyoxyethylene ether Drugs 0.000 claims description 12
- 229920000056 polyoxyethylene ether Polymers 0.000 claims description 12
- 239000003795 chemical substances by application Substances 0.000 claims description 9
- QCDWFXQBSFUVSP-UHFFFAOYSA-N 2-phenoxyethanol Chemical compound OCCOC1=CC=CC=C1 QCDWFXQBSFUVSP-UHFFFAOYSA-N 0.000 claims description 7
- 150000001412 amines Chemical group 0.000 claims description 7
- 229910021538 borax Inorganic materials 0.000 claims description 7
- KGBXLFKZBHKPEV-UHFFFAOYSA-N boric acid Chemical compound OB(O)O KGBXLFKZBHKPEV-UHFFFAOYSA-N 0.000 claims description 7
- 239000004327 boric acid Substances 0.000 claims description 7
- 239000000975 dye Substances 0.000 claims description 7
- 150000003242 quaternary ammonium salts Chemical class 0.000 claims description 7
- 239000004328 sodium tetraborate Substances 0.000 claims description 7
- 235000010339 sodium tetraborate Nutrition 0.000 claims description 7
- 238000000108 ultra-filtration Methods 0.000 claims description 7
- 238000001914 filtration Methods 0.000 claims description 5
- 239000012528 membrane Substances 0.000 claims description 5
- -1 EDTA-K20.2~0.4g/L Substances 0.000 claims description 4
- 230000008676 import Effects 0.000 abstract description 8
- 210000000601 blood cell Anatomy 0.000 abstract description 5
- 102000001554 Hemoglobins Human genes 0.000 abstract description 3
- 108010054147 Hemoglobins Proteins 0.000 abstract description 3
- 210000001772 blood platelet Anatomy 0.000 abstract description 3
- 210000003743 erythrocyte Anatomy 0.000 abstract description 3
- XFXPMWWXUTWYJX-UHFFFAOYSA-N Cyanide Chemical compound N#[C-] XFXPMWWXUTWYJX-UHFFFAOYSA-N 0.000 abstract description 2
- 230000008859 change Effects 0.000 abstract description 2
- 230000036541 health Effects 0.000 abstract description 2
- 238000004519 manufacturing process Methods 0.000 abstract description 2
- 238000000034 method Methods 0.000 abstract description 2
- 230000008569 process Effects 0.000 abstract description 2
- 239000002994 raw material Substances 0.000 abstract description 2
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 4
- 238000009472 formulation Methods 0.000 description 4
- 239000007788 liquid Substances 0.000 description 4
- 239000000203 mixture Substances 0.000 description 4
- YTPLMLYBLZKORZ-UHFFFAOYSA-N Thiophene Chemical compound C=1C=CSC=1 YTPLMLYBLZKORZ-UHFFFAOYSA-N 0.000 description 2
- 150000001336 alkenes Chemical class 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 230000003213 activating effect Effects 0.000 description 1
- 210000004369 blood Anatomy 0.000 description 1
- 239000008280 blood Substances 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- WGCNASOHLSPBMP-UHFFFAOYSA-N hydroxyacetaldehyde Natural products OCC=O WGCNASOHLSPBMP-UHFFFAOYSA-N 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 229930192474 thiophene Natural products 0.000 description 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
-
- G01N15/01—
Abstract
The invention discloses the classification reagent for hemocyte analyzers suit of one kind five, it is made of dilution, hemolytic agent and dyeing liquor.The five classification reagent for hemocyte analyzers of the present invention are set with the features such as formula is reasonable, and raw material is easy to get, easy to process, of low cost, thus, have good value for applications；Change the present situation that domestic reagent for hemocyte analyzers relies on import for a long time, and the hemolytic agent SLS in reagent only greatly reduces the infringement to production unit personnel and experimental implementation person's health for cyanogens-free hemolytic agent, while it also avoid using cyanide pollution on the environment.The five classification Automatic Blood Cell Analyzer, leucocyte, red blood cell, hemoglobin, blood platelet etc. of XN series that the reagent is suitable for Japanese Sysmex company is all higher than 0.95 with import XN reagent related coefficients.
Description
Technical field
The present invention relates to a kind of reagent, is specially the classification reagent for hemocyte analyzers suit its preparation method of one kind five.
Background technology
Five classification Automatic Blood Cell Analyzers are the blood routine detecting instruments that China's popularization uses, but this kind of instrument at present
Device and the reagent to match are import, and the price of import reagent is high, and buying is longer to delivery cycle, to hospital and trouble
Person brings extra financial burden, and brings inconvenience.
The content of the invention
The technical problem to be solved in the present invention is the special agent for overcoming existing five classification Automatic Blood Cell Analyzer
The defects of needing import, there is provided classification reagent for hemocyte analyzers suit of one kind five and preparation method thereof.
In order to solve the above technical problem, the present invention provides following technical solution：
The classification reagent for hemocyte analyzers suit of one kind five, is made of, described is dilute dilution, hemolytic agent and dyeing liquor
Releasing liquid includes：5.5~7g/L of NaCl, 1~1.5g/L of boric acid, EDTA-K20.2~0.4g/L, 0.4~0.6g/L of borax and thiophene
0.3~0.5g/L of oxazoline ketone；
The hemolytic agent includes hemolytic agent-WDF, hemolytic agent-WNR and hemolytic agent-SLS；Hemolytic agent-the WDF includes
NaCl1.8~2.2g/L, EDTA-K20.1~0.3g/L, 1.5~2g/L of polyoxyethylene ether surface active agent, quaternary ammonium salt 0.5~
0.8g/L, 0.5~0.7g/L of succinic acid；Hemolytic agent-the WNR includes 1.0~1.5g/L of quaternary amine, polyoxyethylene ether surface
4.0~5.2g/L of activating agent, 0.4~0.6g/L of succinic acid；EDTA-Na20.1~0.3g/L；Hemolytic agent-the SLS includes：
3.0~3.7g/L of 1.0~1.5g/L of TritonX-100,1.2~1.4g/L of ionic surface active agent and ethylene glycol phenyl ether.
Further, the dilution includes：NaCl 6g/L, boric acid 1.2g/L, EDTA-K20.3g/L, borax
0.5g/L and thiazolinone 0.4g/L.
Further, the hemolytic agent-WDF includes NaCl 2g/L, EDTA-K20.2g/L, polyoxyethylene ether surface are lived
Property agent 1.7g/L, quaternary ammonium salt 0.7g/L, succinic acid 0.55g/L；Hemolytic agent-the WNR includes quaternary amine 1.2g/L, polyoxy second
Alkene ether surface active agent 4.5g/L, succinic acid 0.5g/L；EDTA-Na20.2g/L；Hemolytic agent-the SLS is：TritonX-100
1.2g/L, ionic surface active agent 1.5g/L and ethylene glycol phenyl ether 3.5g/L.
Further, the dyeing liquor includes 0.012~0.03g/L of Polymethine dyes, 28~32g/L of methanol and second
950~1000g/L of glycol.
Further, the dyeing liquor includes Polymethine dyes 0.025g/L, methanol 30g/L and ethylene glycol 970g/
L。
Further, packed after carrying out coarse filtration, ultrafiltration after each agent formulation.Preferably, using 0.22 μm of filter membrane
Carry out ultrafiltration.
The five classification reagent for hemocyte analyzers suit formulas of the present invention are reasonable, and raw material is easy to get, easy to process, cost is low
The features such as honest and clean, thus, have good value for applications；Change domestic reagent for hemocyte analyzers and rely on import for a long time
Present situation, and the hemolytic agent-SLS in reagent is only greatly reduced to production unit personnel and experimental implementation person for cyanogens-free hemolytic agent
The infringement of health, while it also avoid using cyanide pollution on the environment.The reagent is suitable for Japan Xi Senmei
The five classification Automatic Blood Cell Analyzer, leucocyte, red blood cell, hemoglobin, blood platelet etc. of XN series of Kanggong department and import
XN reagent related coefficients are all higher than 0.95.
Embodiment
The preferred embodiment of the present invention is illustrated below, it will be appreciated that preferred embodiment described herein is only used
In the description and interpretation present invention, it is not intended to limit the present invention.
Embodiment 1
The classification reagent for hemocyte analyzers suit of one kind five, is made of, described is dilute dilution, hemolytic agent and dyeing liquor
Releasing liquid includes：NaCl 5.5g/L, boric acid 1g/L, EDTA-K20.2g/L, borax 0.4g/L and thiazolinone 0.3g/L；
The hemolytic agent includes hemolytic agent-WDF, hemolytic agent-WNR and hemolytic agent-SLS；Hemolytic agent-the WDF includes
NaCl 1.8g/L、EDTA-K20.1g/L, polyoxyethylene ether surface active agent 1.5g/L, quaternary ammonium salt 0.5g/L, succinic acid
0.5g/L；Hemolytic agent-the WNR includes quaternary amine 1.0g/L, polyoxyethylene ether surface active agent 4.0g/L, succinic acid
0.4g/L；EDTA-Na20.1g/L；Hemolytic agent-the SLS includes：TritonX-100 1.0g/L, ionic surface active agent
1.2g/L and ethylene glycol phenyl ether 3.0g/L；
Dyeing liquor includes Polymethine dyes 0.012g/L, methanol 28g/L and ethylene glycol 950g/L.
Coarse filtration is first carried out after each agent formulation, is packed after then carrying out ultrafiltration using 0.22 μm of filter membrane.
Embodiment 2
The classification reagent for hemocyte analyzers suit of one kind five, is made of, described is dilute dilution, hemolytic agent and dyeing liquor
Releasing liquid includes：NaCl 7g/L, boric acid 1.5g/L, EDTA-K20.4g/L, borax 0.6g/L and thiazolinone 0.5g/L；
The hemolytic agent includes hemolytic agent-WDF, hemolytic agent-WNR and hemolytic agent-SLS；Hemolytic agent-the WDF includes
NaCl 2.2g/L、EDTA-K20.3g/L, polyoxyethylene ether surface active agent 2g/L, quaternary ammonium salt 0.8g/L, succinic acid 0.7g/
L；Hemolytic agent-the WNR includes quaternary amine 1.5g/L, polyoxyethylene ether surface active agent 5.2g/L, succinic acid 0.6g/L；
EDTA-Na20.3g/L；Hemolytic agent-the SLS includes：TritonX-100 1.5g/L, ionic surface active agent 1.4g/L and
Ethylene glycol phenyl ether 3.7g/L；
Dyeing liquor includes Polymethine dyes 0.03g/L, methanol 32g/L and ethylene glycol 1000g/L.
Coarse filtration is first carried out after each agent formulation, is packed after then carrying out ultrafiltration using 0.22 μm of filter membrane.
Embodiment 3
The classification reagent for hemocyte analyzers suit of one kind five, is made of, described is dilute dilution, hemolytic agent and dyeing liquor
Releasing liquid includes：NaCl 6g/L, boric acid 1.2g/L, EDTA-K20.3g/L, borax 0.5g/L and thiazolinone 0.4g/L.
Further, the hemolytic agent-WDF includes NaCl 2g/L, EDTA-K20.2g/L, polyoxyethylene ether surface are lived
Property agent 1.7g/L, quaternary ammonium salt 0.7g/L, succinic acid 0.55g/L；Hemolytic agent-the WNR includes quaternary amine 1.2g/L, polyoxy second
Alkene ether surface active agent 4.5g/L, succinic acid 0.5g/L；EDTA-Na20.2g/L；Hemolytic agent-the SLS is：TritonX-100
1.2g/L, ionic surface active agent 1.5g/L and ethylene glycol phenyl ether 3.5g/L.
The dyeing liquor includes Polymethine dyes 0.025g/L, methanol 30g/L and ethylene glycol 970g/L.
Coarse filtration is first carried out after each agent formulation, is packed after then carrying out ultrafiltration using 0.22 μm of filter membrane.
Using the five classification reagent for hemocyte analyzers suits and the XN series of Japanese Sysmex company of embodiment 1-3
Five classification Automatic Blood Cell Analyzers are tested, leucocyte, red blood cell, hemoglobin, blood platelet etc. and import XN reagents
Related coefficient is all higher than 0.95, and the correlation of especially embodiment 3 is up to 0.999.
Finally it should be noted that：The foregoing is only a preferred embodiment of the present invention, is not intended to limit the invention,
Although the present invention is described in detail with reference to the foregoing embodiments, for those skilled in the art, it still may be used
To modify to the technical solution described in foregoing embodiments, or equivalent substitution is carried out to which part technical characteristic.
Within the spirit and principles of the invention, any modification, equivalent replacement, improvement and so on, should be included in the present invention's
Within protection domain.
Claims (7)
1. one kind five is classified, reagent for hemocyte analyzers suit, is made of, its feature exists dilution, hemolytic agent and dyeing liquor
In the dilution includes：5.5~7g/L of NaCl, 1~1.5g/L of boric acid, EDTA-K20.2~0.4g/L, borax 0.4
0.3~0.5g/L of~0.6g/L and thiazolinone；The hemolytic agent include hemolytic agent-WDF, hemolytic agent-WNR and hemolytic agent-
SLS；Hemolytic agent-the WDF includes 1.8~2.2g/L of NaCl, EDTA-K20.1~0.3g/L, polyoxyethylene ether surface active
1.5~2g/L of agent, 0.5~0.8g/L of quaternary ammonium salt, 0.5~0.7g/L of succinic acid；Hemolytic agent-the WNR includes quaternary amine 1.0
~1.5g/L, 4.0~5.2g/L of polyoxyethylene ether surface active agent, 0.4~0.6g/L of succinic acid；EDTA-Na20.1~
0.3g/L；Hemolytic agent-the SLS includes：1.0~1.5g/L of TritonX-100,1.2~1.4g/L of ionic surface active agent and
3.0~3.7g/L of ethylene glycol phenyl ether.
2. five classification reagent for hemocyte analyzers suit as claimed in claim 1, it is characterised in that the dilution bag
Include：NaCl 6g/L, boric acid 1.2g/L, EDTA-K20.3g/L, borax 0.5g/L and thiazolinone 0.4g/L.
3. five classification reagent for hemocyte analyzers suit as claimed in claim 1, it is characterised in that the hemolytic agent-WDF
Including NaCl 2g/L, EDTA-K20.2g/L, polyoxyethylene ether surface active agent 1.7g/L, quaternary ammonium salt 0.7g/L, succinic acid
0.55g/L；Hemolytic agent-the WNR includes quaternary amine 1.2g/L, polyoxyethylene ether surface active agent 4.5g/L, succinic acid
0.5g/L；EDTA-Na20.2g/L；Hemolytic agent-the SLS is：TritonX-100 1.2g/L, ionic surface active agent 1.5g/
L and ethylene glycol phenyl ether 3.5g/L.
4. five classification reagent for hemocyte analyzers suit as claimed in claim 1, it is characterised in that the dyeing liquor bag
Include 0.012~0.03g/L of Polymethine dyes, 950~1000g/L of 28~32g/L of methanol and ethylene glycol.
5. five classification reagent for hemocyte analyzers suit as claimed in claim 4, it is characterised in that the dyeing liquor bag
Include Polymethine dyes 0.025g/L, methanol 30g/L and ethylene glycol 970g/L.
6. the preparation method of five classification reagent for hemocyte analyzers suits described in claim 1, it is characterised in that each reagent
Packed after carrying out coarse filtration, ultrafiltration after dispensing.
7. the preparation method of five classification reagent for hemocyte analyzers suits described in claim 6, it is characterised in that use
0.22 μm of filter membrane carries out ultrafiltration.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711177105.4A CN107941661A (en) | 2017-11-22 | 2017-11-22 | Classification reagent for hemocyte analyzers suit of one kind five and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711177105.4A CN107941661A (en) | 2017-11-22 | 2017-11-22 | Classification reagent for hemocyte analyzers suit of one kind five and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107941661A true CN107941661A (en) | 2018-04-20 |
Family
ID=61929910
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711177105.4A Pending CN107941661A (en) | 2017-11-22 | 2017-11-22 | Classification reagent for hemocyte analyzers suit of one kind five and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107941661A (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110470587A (en) * | 2019-08-12 | 2019-11-19 | 江苏美诚生物科技有限公司 | Five classification cellanalyzer of one kind is counted with basophilic granulocyte to be set with |
| CN110542640A (en) * | 2019-08-12 | 2019-12-06 | 江苏美诚生物科技有限公司 | Leukocyte classification kit for five-classification blood cell analyzer |
| CN111521834A (en) * | 2020-03-20 | 2020-08-11 | 佛山市顺德区德维医疗器械有限公司 | Reagent for XN series full-automatic modular blood body fluid analyzer |
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| US20020098589A1 (en) * | 2001-01-19 | 2002-07-25 | Crews Harold Richardson | Multi-purpose reagent system and method for enumeration of red blood cells, white blood cells and thrombocytes and differential determination of white blood cells |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN110470587A (en) * | 2019-08-12 | 2019-11-19 | 江苏美诚生物科技有限公司 | Five classification cellanalyzer of one kind is counted with basophilic granulocyte to be set with |
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| CN111521834A (en) * | 2020-03-20 | 2020-08-11 | 佛山市顺德区德维医疗器械有限公司 | Reagent for XN series full-automatic modular blood body fluid analyzer |
| CN111521834B (en) * | 2020-03-20 | 2023-03-24 | 佛山市顺德区德维医疗科技有限公司 | Reagent for XN series full-automatic modular blood body fluid analyzer |
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