CN107912565A - A kind of method for synchronously preparing tealeaves concentrate and tea aroma substance - Google Patents

A kind of method for synchronously preparing tealeaves concentrate and tea aroma substance Download PDF

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Publication number
CN107912565A
CN107912565A CN201711383260.1A CN201711383260A CN107912565A CN 107912565 A CN107912565 A CN 107912565A CN 201711383260 A CN201711383260 A CN 201711383260A CN 107912565 A CN107912565 A CN 107912565A
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tea
concentrate
tealeaves
aroma substance
enzyme
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CN107912565B (en
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汪少芸
岳鹏翔
陈旭
蔡茜茜
田永奇
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Fuzhou University
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Fuzhou University
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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23F—COFFEE; TEA; THEIR SUBSTITUTES; MANUFACTURE, PREPARATION, OR INFUSION THEREOF
    • A23F3/00—Tea; Tea substitutes; Preparations thereof
    • A23F3/16—Tea extraction; Tea extracts; Treating tea extract; Making instant tea
    • A23F3/166—Addition of, or treatment with, enzymes or microorganisms
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23F—COFFEE; TEA; THEIR SUBSTITUTES; MANUFACTURE, PREPARATION, OR INFUSION THEREOF
    • A23F3/00—Tea; Tea substitutes; Preparations thereof
    • A23F3/16—Tea extraction; Tea extracts; Treating tea extract; Making instant tea
    • A23F3/163—Liquid or semi-liquid tea extract preparations, e.g. gels, liquid extracts in solid capsules
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23F—COFFEE; TEA; THEIR SUBSTITUTES; MANUFACTURE, PREPARATION, OR INFUSION THEREOF
    • A23F3/00—Tea; Tea substitutes; Preparations thereof
    • A23F3/40—Tea flavour; Tea oil; Flavouring of tea or tea extract
    • A23F3/42—Isolation or recuperation of tea flavour or tea oil

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Polymers & Plastics (AREA)
  • Microbiology (AREA)
  • Dispersion Chemistry (AREA)
  • Oil, Petroleum & Natural Gas (AREA)
  • Tea And Coffee (AREA)

Abstract

The present invention relates to a kind of method for synchronously preparing tealeaves concentrate and tea aroma substance, belong to food processing technology field, tealeaves into horizontal high voltage broken wall and is recycled by tea aroma substance using twin-screw extruder and vacuum critical point concentration technique, tea polyphenols and aromatic substance in extracting tealeaves under conditions of room temperature by complex enzyme hydrolysis technique, realize the holding of high extraction and tea special aroma, while prepare natural scents material in tea concentrate and tealeaves.The present invention is by a variety of advanced technology conjunctive uses, thus it is possible to vary the dependence to heating extraction, cold leaching cooling concentration, reduces the cost of production；And make full use of the water-insoluble albumen and cellulose enriched in tealeaves, environmental pollution can not only be reduced and can also create huge Social benefit and economic benefit by carrying out deep development and comprehensive utilization to tea leaf protein and cellulose, and the development to science and technology, economy and grocery trade has far-reaching significance.

Description

A kind of method for synchronously preparing tealeaves concentrate and tea aroma substance
Technical field
Present invention relates particularly to a kind of method for synchronously preparing tealeaves concentrate and tea aroma substance, belong to food processing skill Art field.
Background technology
Tea-leaf beverage widely loved by the majority of consumers two it is big the reason is that：Anti-oxidation health effect and tealeaves of tea polyphenols are only Special aromatic odor.As tea concentrate, the former the i.e. content of tea polyphenols and the problems such as tea polyphenols oxidation protection has been obtained for Solve well, it is still, also readily volatilized since the aromatic odor of tealeaves is sufficiently complex, it is easy to be broken in process Bad or forfeiture.Therefore, the bottleneck problem for influencing tea concentrate and related tea beverage development at present is how to obtain real tea The characteristics of fragrant concentrate, holding original tealeaves.The production key technology of current tealeaves " Luzhou-flavor " concentrate is solved, tealeaves is " dense Odor type " concentrate research and develop with having far-reaching meaning using the sustainable development to our province tea industry and China's tea culture industry Justice.
In addition, the continuous development of tea beverage industry, substantial amounts of water, which carries tealeaves, to be discarded tea grounds and appears in our life.No The significant wastage of resource is only caused, and certain pollution is caused to environment.How discarded tea grounds is rationally handled, make tea Slag is turned waste into wealth, and is conducive to environmental protection, improves the reuse ratio of resource, has caused the concern and attention of various circles of society.Tealeaves In contain more protein, its content accounts for the 15%~30% of dry weight of tea leaves, wherein most protein is water-insoluble Albumen, mainly including glutelin, alcohol soluble protein, albumin, globulin.Water-insoluble tea is extracted using the heavy method of alkali carries acid Residue protein, studies the antioxidation activity in vitro of tea grounds polypeptide compound after enzymatic hydrolysis, and with traditional synthesis Antioxidant Vc, BHT carry out the comparison of antioxidant effect, and a kind of new, green, natural antioxygen is developed using discarded tea grounds Agent, and applied in company's series beverage, there is very high economic value and social value.
Dietary fiber refers to that can suppress human small intestine digests and assimilates, and it is edible partly or entirely to ferment in human body large intestine The summation of vegetable ingredients, carbohydrate and its similar material.Mainly include polysaccharide, oligosaccharides, lignin and phase The plant material of pass.Dietary fiber can be divided into water-soluble dietary fiber according to deliquescent size and water-insoluble meals are fine Dimension.Water insoluble dietary fiber (mainly including cellulose, hemicellulose, lignin etc.) has relatively good water imbibition and swollen Swollen property, it is possible to promote small bowel peristalsis, so as to reach the effect of Constipation and intestines problem；In addition, water-insoluble meals are fine Dimension can also increase satiety, achieve the purpose that to prevent obesity；And can also to adsorb enterotoxin right for Water insoluble dietary fiber Enterotoxin is promoted to excrete afterwards.Water insoluble dietary fiber is one of main component that water carries discarded tea grounds.Water is carried discarded Tea grounds is processed as smalls, as food additives, is added in company's series beverage, has widened the utilization that water carries discarded tea grounds, Have great importance.
The content of the invention
The present invention provides a kind of method for synchronously preparing tealeaves concentrate and tea aroma substance, using twin-screw extruder and very Empty critical point concentration technique by tealeaves into horizontal high voltage broken wall and recycling tea aroma substance, by complex enzyme hydrolysis technique room temperature bar The tea polyphenols and aromatic substance in tealeaves are extracted under part, realize the holding of high extraction and tea special aroma, while prepare tea Natural scents material in concentrate and tealeaves.
To achieve the above object, the present invention adopts the following technical scheme that：
A kind of method for synchronously preparing tealeaves concentrate and tea aroma substance, includes the following steps：
（1）Broken wall：Broken wall treatment is carried out to tealeaves using Twin-Screw Extrusion Technology；
（2）Enzymolysis：Limited enzyme is carried out to sedimentable protein, dietary fiber under conditions of room temperature by complex enzyme zymohydrolysis technique Solution；
（3）Filtering：Tea enzymolysis liquid is filtered using disk centrifugation technique, improves the service efficiency of ultrafiltration membrane, shortens solid particle（Or Drop）Settling height, expand rotary drum settling area, the production capacity of seperator is improved, so as to be conducive to scale metaplasia Production；
（4）Normal pressure critical point is fractionated：By step（3）Obtained enzymolysis liquid is concentrated into original volume through normal pressure critical point separation 80% ~ 90%, obtain thick concentrate 1 and low boiling point liquid fractionation；
（5）Vacuum critical point is fractionated：By step（4）Obtained thick concentrate 1 carries out vacuum critical point separation and is concentrated into original body Long-pending 50% ~ 60%, obtains tea to concentrate 2 and higher boiling liquid fractionation；
（6）Tea perfume gas component collection：To step（4）Low boiling point liquid fractionation and step（5）Vacuum critical point liquid fractionation passes through condensation Recycled, then by resin adsorption, alcohol elution, obtains tea fragrance component；
（7）Sterilization：To step（5）Tea concentrate finished product is made by the way of radiation sterilizing in obtained tea concentrate 2.
Step（1）Used twin-screw revolves extrusion technique broken wall parameter：Measuring Moisture Content of Tea content 10 ~ 20%, barrel zone temperature 150 ~ 200 DEG C, screw speed is 100 ~ 130 r/min, 80 ~ 100 kg/h of charging rate.
Step（2）Used enzyme solution is complex enzyme hydrolysis, and tea mass volume ratio is 1:20~1:50g/mL, it is used Enzyme is pectase, cellulase and acid protease synergistic effect, and wherein pectinase activity is the U/g of 10,000 U/g ~ 30,000, additive amount For 0.1% ~ 0.5%；Cellulase activity is the U/g of 10,000 U/g ~ 20,000, and additive amount is 0.05% ~ 0.1%；Acid protease activity is 2000U/g ~ 8000U/g, additive amount are 0.5% ~ 5.5%.It is 50 ~ 60 DEG C in temperature, when pH is that 3 ~ 6 times enzymolysis 3 ~ 5 are small, boiling water Bathe and 10 ~ 15min is centrifuged under enzyme deactivation 10 ~ 20min, 6000 ~ 10000rpm, obtain tea enzymolysis liquid.
Step（3）Used filtering uses disk centrifugation technique, and centrifuging temperature is 50 ~ 60 DEG C.
Step（4）Filtered fluid is once concentrated using normal pressure critical point fractionation.
Step（5）Secondary concentration is carried out to filtered fluid using vacuum critical point fractionation.
Step（6）Fragrance component is collected and uses condensing reflux, obtains condensate liquid and is afforded again by resin adsorption, alcohol Natural tea aroma substance.
Step（7）Sterilization uses radiation sterilizing technology.
The remarkable advantage of the present invention is：
（1）Tealeaves twin-screw extruder wall breaking technology, tea powder complex enzyme hydrolysis technology, tea filtrate ultrafiltration and film concentration technique are proposed first A variety of advanced technology conjunctive uses such as concentration technique are separated with tea leaching liquor vacuum critical point, entirely application is set by this project Meter, thus it is possible to vary the dependence to heating extraction, cold leaching cooling concentration, reduces the cost of production, increases the competitiveness of enterprise.
（2）Make full use of the water containing abundant tea leaf protein and cellulose to carry tealeaves and discard tea grounds, carrying tealeaves with water discards Tea grounds is raw material, carry out deep development to tea leaf protein and cellulose and comprehensive utilization can not only reduce environmental pollution can be with Huge Social benefit and economic benefit is createed, the development to science and technology, economy and grocery trade has far-reaching significance.
（3）Room temperature extraction that the present invention uses, vacuum critical point concentration, radiation sterilizing technology farthest avoid hot place Reason is to the destructiveness of millet paste and the loss of fragrance.
Embodiment
Embodiment 1
A kind of method for synchronously preparing tealeaves concentrate and tea aroma substance, comprises the following steps that：
（1）Broken wall：Broken wall treatment is carried out to tealeaves using Twin-Screw Extrusion Technology；
（2）Enzymolysis：Limited enzyme is carried out to sedimentable protein, dietary fiber under conditions of room temperature by complex enzyme zymohydrolysis technique Solution；
（3）Filtering：Tea enzymolysis liquid is filtered using disk centrifugation technique, improves the service efficiency of ultrafiltration membrane, shortens solid particle（Or Drop）Settling height, expand rotary drum settling area, the production capacity of seperator is improved, so as to be conducive to scale metaplasia Production；
（4）Normal pressure critical point separation concentration：By step（3）Obtained enzymolysis liquid is concentrated into original volume through normal pressure critical point separation 90%, obtain thick concentrate 1 and low boiling point liquid fractionation；
（5）Vacuum critical point is fractionated：By step（4）Obtained thick concentrate 1 carries out vacuum critical point separation and is concentrated into original body Long-pending 60%, obtains tea to concentrate 2 and higher boiling liquid fractionation；
（6）Tea perfume gas component collection：To step（4）Low boiling point liquid fractionation and step（5）Vacuum critical point liquid fractionation passes through condensation Recycled, then by resin adsorption, alcohol elution, obtains tea fragrance component；Tea perfume gas components recoveries are 2.0%；
（7）Sterilization：To step（5）Tea concentrate finished product is made by the way of radiation sterilizing in obtained tea concentrate 2.
Step（1）Used twin-screw revolves extrusion technique broken wall parameter：Measuring Moisture Content of Tea content 10%, barrel zone temperature 150 DEG C, screw speed is 100 r/min, charging rate 80kg/h.
Step（2）Used enzyme solution is complex enzyme zymohydrolysis, tea-water proportion 1:20g/mL, enzyme used are 10,000 U/g's The acid protease synergistic effect of pectase, the cellulase of 10,000 U/g and 2000U/g, enzyme additive amount is respectively 0.1%, 0.05% With 0.5%, temperature is 50 DEG C, pH for digest for 5 time 3 it is small when, centrifuge 10min under boiling water bath enzyme deactivation 10min, 6000rpm, obtain tea Slag hydrolyzate.
Step（3）Used filtering uses disk centrifugation technique, and centrifuging temperature is 50 DEG C.
Embodiment 2
A kind of method for synchronously preparing tealeaves concentrate and tea aroma substance, includes the following steps：
（1）Broken wall：Broken wall treatment is carried out to tealeaves using Twin-Screw Extrusion Technology；
（2）Enzymolysis：Limited enzyme is carried out to sedimentable protein, dietary fiber under conditions of room temperature by complex enzyme zymohydrolysis technique Solution；
（3）Filtering：Tea enzymolysis liquid is filtered using disk centrifugation technique, improves the service efficiency of ultrafiltration membrane, shortens solid particle（Or Drop）Settling height, expand rotary drum settling area, the production capacity of seperator is improved, so as to be conducive to scale metaplasia Production；
（4）Normal pressure critical point is fractionated：By step（3）Obtained enzymolysis liquid is concentrated into original volume through normal pressure critical point separation 80%, obtain thick concentrate 1 and low boiling point liquid fractionation；
（5）Vacuum critical point is fractionated：By step（4）Obtained thick concentrate 1 carries out vacuum critical point separation and is concentrated into original body Long-pending 50%, obtains tea to concentrate 2 and higher boiling liquid fractionation；
（6）Tea perfume gas component collection：To step（4）Low boiling point liquid fractionation and step（5）Vacuum critical point liquid fractionation passes through condensation Recycled, then by resin adsorption, alcohol elution, obtains tea fragrance component；Tea perfume gas components recoveries are 2.2%；
（7）Sterilization：To step（5）Tea concentrate finished product is made by the way of radiation sterilizing in obtained tea concentrate 2.
Step（1）Used twin-screw revolves extrusion technique broken wall parameter：Measuring Moisture Content of Tea content 20%, barrel zone temperature 200 DEG C, screw speed is 130 r/min, 100 kg/h of charging rate.
Step（2）Used enzyme solution is complex enzyme zymohydrolysis, is 1 in tea-water proportion:50 g/mL, enzyme used are 30,000 U/ The acid protease synergistic effect of the pectase of g, the cellulase of 20,000 U/g and 8000U/g, enzyme additive amount is respectively 0.5%, 0.1% and 0.5%, it is 60 DEG C in temperature, when pH is that 6 times enzymolysis 5 are small, 15min is centrifuged under boiling water bath enzyme deactivation 15min, 8000rpm, Obtain tea grounds hydrolyzate.
Step（3）Used filtering uses disk centrifugation technique, and centrifuging temperature is 60 DEG C.
The foregoing is merely the preferable embodiment of the present invention, all equivalent changes done according to scope of the present invention patent With modification, it should all belong to covering scope of the invention.

Claims (4)

  1. A kind of 1. method for synchronously preparing tealeaves concentrate and tea aroma substance, it is characterised in that comprise the following steps：
    （1）Broken wall：Broken wall treatment is carried out to tealeaves using Twin-Screw Extrusion Technology；
    （2）Enzymolysis：Limited enzyme is carried out to sedimentable protein, dietary fiber under conditions of room temperature by complex enzyme zymohydrolysis technique Solution；
    （3）Filtering：Using disk centrifugal filtration tea enzymolysis liquid；
    （4）Normal pressure critical point separation concentration：By step（3）Obtained enzymolysis liquid is concentrated into original volume through normal pressure critical point separation 80% ~ 90%, obtain thick concentrate 1 and low boiling point liquid fractionation；
    （5）Vacuum critical point separation concentration：By step（4）Obtained thick concentrate 1 carries out vacuum critical point separation and is concentrated into original Carry out the 50% ~ 60% of volume, obtain tea to concentrate 2 and higher boiling liquid fractionation；
    （6）Tea perfume gas component collection：To step（4）Low boiling point liquid fractionation and step（5）Higher boiling liquid fractionation passes through condensing reflux Recycled, then by resin adsorption, alcohol elution, obtains tea aroma substance；
    （7）Sterilization：To step（5）Tealeaves concentrate finished product is made by the way of radiation sterilizing in obtained tea concentrate 2.
  2. A kind of 2. method for synchronously preparing tealeaves concentrate and tea aroma substance according to claim 1, it is characterised in that Step（1）Used twin-screw revolves extrusion technique broken wall parameter：Measuring Moisture Content of Tea content 10 ~ 20%, barrel zone temperature 150 ~ 200 DEG C, screw speed is 100 ~ 130 r/min, 80 ~ 100 kg/h of charging rate.
  3. A kind of 3. method for synchronously preparing tealeaves concentrate and tea aroma substance according to claim 1, it is characterised in that： Step（2）Used enzyme solution is complex enzyme hydrolysis, and tea mass volume ratio is 1:20~1:50g/mL, enzyme used are pectin Enzyme, cellulase and acid protease synergistic effect, wherein pectinase activity are the U/g of 10,000 U/g ~ 30,000, additive amount for 0.1% ~ 0.5%；Cellulase activity is the U/g of 10,000 U/g ~ 20,000, and additive amount is 0.05% ~ 0.1%；Acid protease activity for 2000U/g ~ 8000U/g, additive amount are 0.5% ~ 5.5%；It is 50 ~ 60 DEG C in temperature, when pH is that 3 ~ 6 times enzymolysis 3 ~ 5 are small, boiling water bath enzyme deactivation 10 ~ 10 ~ 15min is centrifuged under 20min, 6000 ~ 10000rpm, obtains tea enzymolysis liquid.
  4. A kind of 4. method for synchronously preparing tealeaves concentrate and tea aroma substance according to claim 1, it is characterised in that： Step（3）Filtering uses disk centrifugation technique, and centrifuging temperature is 50 ~ 60 DEG C.
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CN111587976A (en) * 2020-05-19 2020-08-28 王中华 Method for extracting bioactive functional components from selenium-rich tea and application thereof

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Publication number Priority date Publication date Assignee Title
CN111587976A (en) * 2020-05-19 2020-08-28 王中华 Method for extracting bioactive functional components from selenium-rich tea and application thereof
CN111587976B (en) * 2020-05-19 2022-05-27 王中华 Method for extracting bioactive functional components from selenium-rich tea and application thereof

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