CN107907696A - A kind of detection plate and identification method based on the quick bracket for blood grouping of solid phase method - Google Patents
A kind of detection plate and identification method based on the quick bracket for blood grouping of solid phase method Download PDFInfo
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- CN107907696A CN107907696A CN201711391282.2A CN201711391282A CN107907696A CN 107907696 A CN107907696 A CN 107907696A CN 201711391282 A CN201711391282 A CN 201711391282A CN 107907696 A CN107907696 A CN 107907696A
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- 210000004369 blood Anatomy 0.000 title claims abstract description 52
- 239000008280 blood Substances 0.000 title claims abstract description 52
- 238000001514 detection method Methods 0.000 title claims abstract description 19
- 238000010532 solid phase synthesis reaction Methods 0.000 title claims abstract description 11
- 238000000034 method Methods 0.000 title claims abstract description 10
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 claims abstract description 73
- 239000010931 gold Substances 0.000 claims abstract description 72
- 229910052737 gold Inorganic materials 0.000 claims abstract description 72
- 238000002615 hemofiltration Methods 0.000 claims abstract description 18
- 239000003153 chemical reaction reagent Substances 0.000 claims abstract description 16
- 210000003617 erythrocyte membrane Anatomy 0.000 claims abstract description 15
- 101710181478 Envelope glycoprotein GP350 Proteins 0.000 claims abstract description 14
- 210000003743 erythrocyte Anatomy 0.000 claims abstract description 11
- 210000002381 plasma Anatomy 0.000 claims description 9
- 238000006243 chemical reaction Methods 0.000 claims description 8
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 4
- 239000000427 antigen Substances 0.000 abstract description 6
- 102000036639 antigens Human genes 0.000 abstract description 6
- 108091007433 antigens Proteins 0.000 abstract description 6
- 239000003795 chemical substances by application Substances 0.000 abstract description 5
- 239000000084 colloidal system Substances 0.000 abstract description 3
- 238000010241 blood sampling Methods 0.000 abstract description 2
- 230000000694 effects Effects 0.000 description 3
- FGRBYDKOBBBPOI-UHFFFAOYSA-N 10,10-dioxo-2-[4-(N-phenylanilino)phenyl]thioxanthen-9-one Chemical compound O=C1c2ccccc2S(=O)(=O)c2ccc(cc12)-c1ccc(cc1)N(c1ccccc1)c1ccccc1 FGRBYDKOBBBPOI-UHFFFAOYSA-N 0.000 description 1
- TVEXGJYMHHTVKP-UHFFFAOYSA-N 6-oxabicyclo[3.2.1]oct-3-en-7-one Chemical compound C1C2C(=O)OC1C=CC2 TVEXGJYMHHTVKP-UHFFFAOYSA-N 0.000 description 1
- 102100027544 Blood group Rh(D) polypeptide Human genes 0.000 description 1
- 206010018910 Haemolysis Diseases 0.000 description 1
- 101000580024 Homo sapiens Blood group Rh(D) polypeptide Proteins 0.000 description 1
- 208000003441 Transfusion reaction Diseases 0.000 description 1
- 238000007818 agglutination assay Methods 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000010836 blood and blood product Substances 0.000 description 1
- 229940125691 blood product Drugs 0.000 description 1
- 238000009582 blood typing Methods 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 238000013467 fragmentation Methods 0.000 description 1
- 238000006062 fragmentation reaction Methods 0.000 description 1
- QUCZBHXJAUTYHE-UHFFFAOYSA-N gold Chemical compound [Au].[Au] QUCZBHXJAUTYHE-UHFFFAOYSA-N 0.000 description 1
- 230000008588 hemolysis Effects 0.000 description 1
- 238000000338 in vitro Methods 0.000 description 1
- 239000007791 liquid phase Substances 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 239000012071 phase Substances 0.000 description 1
- 230000008092 positive effect Effects 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 238000012797 qualification Methods 0.000 description 1
- 238000012827 research and development Methods 0.000 description 1
- 239000007790 solid phase Substances 0.000 description 1
- 238000001179 sorption measurement Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 230000017260 vegetative to reproductive phase transition of meristem Effects 0.000 description 1
- 230000000007 visual effect Effects 0.000 description 1
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/80—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood groups or blood types or red blood cells
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Hematology (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Immunology (AREA)
- Urology & Nephrology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Food Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The present invention relates to a kind of detection plate and identification method based on the quick bracket for blood grouping of solid phase method, detection plate includes plate body, the sample slot being arranged in the middle part of plate body, is arranged on the antibody groove of sample slot side and is arranged on the gold mark groove of sample slot opposite side, there is monoclonal antibody in antibody groove, gold mark erythrocyte membrane antigen is provided with gold mark groove, blotting paper on being provided between the sample slot and antibody groove, hemofiltration film is provided between sample slot and gold mark groove, lower blotting paper is provided with below hemofiltration film；The present invention uses the anti-A of specific monoclonal, anti-B, anti-D as abo blood group positive definite form reagent, using mark specific erythrocyte film A, B, O blood group antigens（Gold mark antigen）As reverse type immune colloid gold reagent, positive and negative styling agent is arranged on plate body, simple and quick, easy to carry, and accuracy is high, and positive and negative styling agent is placed in quick-fried pearl blood grouping reagent when being adapted to flowing blood sampling point and emergent blood transfusion at the same time.
Description
Technical field
The present invention relates to a kind of detection plate and identification method based on the quick bracket for blood grouping of solid phase method.
Background technology
Modern war has more sudden, uncertain and miserable property compared with conventional war.Due to new-type High Fragmentation weapon
Continuous research and development and commonly used, the quantity for causing to be badly in need of the severely injured people that blood transfusion is given treatment to sharply increases.Meanwhile in order to
Ensure that various blood products are transfused effective and wartime blood use safety, it is necessary to strictly observe bracket for blood grouping, cross matching before blood transfusion
Etc. the standard practice instructions requirement of various detections, this under the conditions of field operation or when accident be difficult to ensure, if blood group
Blood grouping and crossmatching inaccuracy this may result in the wounded and miss defeated special-shaped blood and serious transfusion reaction occur, or even can crisis life
Life.How my army implements the important content that strong blood transfusion treatment is military struggle health support in future war.By right
Domestic and international all previous war analysis, the motor-driven combat exercise of multiple health support organized with reference to my army and Wenchuan earthquake etc. are natural
Take a hint in disaster assistance：Clinical common bracket for blood grouping method has flowering in vitro and micro-column gel agglutination assay at present, required
Detection reagent requires low temperature refrigerator to preserve, and experimental implementation needs centrifuge, is required for supply of electric power, and the latter's qualification time compared with
It is long, it is also necessary to which that dedicated instrument and reagent, and higher price, both of which are not suitable for field under emergency circumstances and use.
The content of the invention
The technical problems to be solved by the invention are to provide a kind of be suitable in wartime Rapid identification blood group based on solid phase method
The detection plate and identification method of quick bracket for blood grouping.
The technical solution adopted in the present invention is：
A kind of detection plate based on the quick bracket for blood grouping of solid phase method, it includes plate body, the sample slot being arranged in the middle part of plate body, sets
Sample slot side antibody groove and be arranged on sample slot opposite side gold mark groove, have monoclonal antibody in antibody groove, gold mark groove
Gold mark erythrocyte membrane antigen is inside provided with, blotting paper is provided between the sample slot and antibody groove, in sample slot and gold
Hemofiltration film is provided between mark groove, lower blotting paper is provided with below hemofiltration film.
The monoclonal antibody is anti-A, anti-B, anti-D, and the antibody groove is three, is respectively arranged with three antibody grooves anti-
A, anti-B, anti-D, three antibody grooves are connected by upper blotting paper with sample slot respectively；
The gold mark erythrocyte membrane antigen is gold mark A films Ag, gold mark B films Ag, gold mark C films Ag, and the gold mark groove is three, three
Gold mark A films Ag, gold mark B films Ag, gold mark C films Ag are respectively arranged with a gold mark groove, three gold mark grooves pass through lower suction respectively
Water paper is connected with sample slot.
Quick-fried pearl is both provided with the antibody groove with gold mark groove, the monoclonal antibody is arranged at gold mark erythrocyte membrane antigen
In quick-fried pearl.
Described upper blotting paper one end is arranged in antibody groove, and the other end is arranged on inside sample slot or crosses sample slot.
Described lower blotting paper one end is arranged in gold mark groove, and the other end is arranged on inside sample slot or crosses sample slot.
The upper blotting paper is arranged above hemofiltration film.
The method of above-mentioned detection plate, it includes the following steps：
Step 1：Antibody groove and the quick-fried pearl in gold mark groove are racked, flow out the reagent in quick-fried pearl；
Step 2：Whole blood sample is added in sample slot, under the action of upper blotting paper whole blood to antibody groove move, red blood cell with
Monoclonal antibody reacts, and carries out positive definite form；The blood plasma after red blood cell is filtered out under the action of hemofiltration film under the action of lower blotting paper to gold
Groove movement, blood plasma and gold mark erythrocyte membrane antigen reaction are marked, carries out reverse type；
Step 3：Reaction result in observation of steps two, according to positive reverse type aggegation result judgement blood group.
The positive effect of the present invention is：The present invention is using the anti-A of specific monoclonal, anti-B, Antibodies Against Rhesus D Antigen as ABO blood
Type positive definite form reagent, using mark specific erythrocyte film A, B, O blood group antigens（Gold mark antigen）Colloid is immunized as reverse type
Gold reagent, positive and negative styling agent is arranged on plate body, simple and quick, easy to carry, and accuracy is high, is adapted to flowing blood sampling
Positive and negative setting agent is placed in quick-fried pearl at the same time for blood typing when point and emergent blood transfusion, when using when quick-fried pearl need to only be racked i.e.
Can, reagent in quick-fried pearl can room temperature, it is long-term preserve, solve to cut off the water supply under the conditions of field operation, power off, equipment reagent can not supply in time
It is how effectively great for the practical problem for wounded's blood transfusion of losing blood, military significance in the case of answering.
Brief description of the drawings
Fig. 1 is schematic structural view of the invention；
Fig. 2 is sectional view of the present invention.
Embodiment
As shown in attached drawing 1,2, detection plate of the invention includes plate body 1, the sample slot 5 positioned at the middle part of plate body 1, is arranged on sample
The antibody groove 2 of 5 side of this groove and the gold mark groove 3 for being arranged on 5 opposite side of sample slot, have monoclonal antibody in antibody groove 2, groove are marked in gold
Gold mark erythrocyte membrane antigen is provided with 3, blotting paper 7 is provided between the sample slot 5 and antibody groove 2, in sample slot 5
Hemofiltration film 8 is provided between marking groove 3 with gold, lower blotting paper 9 is provided with below hemofiltration film 8.In the present embodiment, antibody groove 2
Positioned at the left side of sample slot 5, the blood sample in sample slot 5 is under the action of upper blotting paper 7, and whole blood is moved into antibody groove 2, whole blood
Interior red blood cell carries out positive definite form with monoclonal antibody reaction.Gold mark groove 3 is located at the right side of sample slot 5, and the whole blood in sample slot 5 is filtered
The filtering of blood film 8, blood plasma under the action of lower blotting paper 9 to gold mark groove 3 move, blood plasma with gold mark erythrocyte membrane antigen react into
Row reverse type.
The monoclonal antibody is anti-A, anti-B, anti-D, and the antibody groove 2 is three, is respectively arranged with three antibody grooves 2
Anti- A, anti-B, anti-D, three antibody grooves 2 are connected by upper blotting paper 7 with sample slot 5 respectively.The gold mark erythrocyte membrane
Antigen is gold mark A films Ag, gold mark B films Ag, gold mark C films Ag, and the gold mark groove 3 is three, is set respectively in three gold mark grooves 3
There are gold mark A films Ag, gold mark B films Ag, gold mark C films Ag, three gold mark grooves 3 are connected by lower blotting paper 9 with sample slot 5 respectively
It is logical.
Quick-fried pearl 4 is both provided with the antibody groove 2 with gold mark groove 3, the monoclonal antibody is all provided with gold mark erythrocyte membrane antigen
Put in quick-fried pearl 4.
Described 7 one end of upper blotting paper is arranged in antibody groove 2, and the other end is arranged on the inside of sample slot 5 or crosses sample slot
5, described lower 9 one end of blotting paper is arranged in gold mark groove 3, and the other end is arranged on the inside of sample slot 5 or crosses sample slot 5.
When one in upper blotting paper 7 or hemofiltration film 8 crosses sample slot 5, upper blotting paper 7 portion overlapping with hemofiltration film
Point, upper blotting paper 7 is arranged at the top of hemofiltration film 8.
Groove 6 can be set between sample slot 5 and antibody groove 2 and gold mark groove 3, upper blotting paper 7 is respectively provided with hemofiltration film 8
In groove 6.
The application method of above-mentioned detection plate includes the following steps：
Step 1：Antibody groove 2 and the quick-fried pearl 4 in gold mark groove 3 are racked, flow out the reagent in quick-fried pearl 4；
Step 2：Whole blood sample is added in sample slot 5, whole blood is moved to antibody groove 2 under the action of upper blotting paper 7, red thin
Born of the same parents react with monoclonal antibody, carry out positive definite form；Effect of the blood plasma after red blood cell in lower blotting paper 9 is filtered out under the action of hemofiltration film 8
Lower to be moved to gold mark groove 3, blood plasma and gold mark erythrocyte membrane antigen reaction, carry out reverse type；
Step 3：Reaction result in observation of steps two, according to positive reverse type aggegation result judgement blood group.
The present invention wraps up the monoclonal antibody specific of high-titer into microballoon, it is ensured that ABO positive definite form result of the tests
Specificity and have good stability, reverse type has complete blood using immune colloid gold reagent by what colloidal gold particle was tagged to extraction
On the erythrocyte membrane of type antigen, solve the problems, such as that the easy haemolysis of conventional reverse type normal erythrocytes, storage life are short, and be beneficial to blood group
Positive reverse type synchronously carries out, and realizes normalizing operation, and positive and negative styling agent is pre-coated in microballoon, only need to be during practical application
The whole blood of examinee is added in loading slot, by the effect of quick adsorption and hemofiltration film, red blood cell enters positive definite form side and monoclonal antibody
Seroreaction, the blood plasma that filtered blood film 8 filters off red blood cell enters reverse type side and gold mark membranous antigen reaction, according to aggegation result
Judge blood group.The present invention has the following advantages:1. it is convenient that reagent preserves, traditional reagent needs 2-8 DEG C of preservation, which can
Room temperature preserves;2. easy to carry, pollution is small, the shortcomings that overcoming liquid-phase reagent easy spilling, and operation scene can keep clear
It is clean;3. simple and quick, without instrument, power and equipment guarantee is not required in experiment, and visual result, is easy to interpretation, non-
Professional can implement, can sentence read result within 2min;4. stability is good, effect phase length;5. it is easy on solid phase carrier viscous
Joint strip shape code, can be to avoid error;6. detection card is detachable, it is easy to carry about with one and preserves, with takes with easy to use.Therefore,
In the field emergency blood security of water shortage power-off bracket for blood grouping accuracy is greatly improved with the method.
Claims (6)
1. a kind of detection plate based on the quick bracket for blood grouping of solid phase method, it is characterised in that it includes plate body（1）, be arranged on plate body
（1）The sample slot at middle part（5）, be arranged on sample slot（5）The antibody groove of side（2）And it is arranged on sample slot（5）Opposite side
Gold mark groove（3）, in antibody groove（2）Inside there is monoclonal antibody, groove is marked in gold（3）Gold mark erythrocyte membrane antigen is inside provided with, in the sample
Groove（5）With antibody groove（2）Between be provided with blotting paper（7）, in sample slot（5）Groove is marked with gold（3）Between be provided with hemofiltration film
（8）, in hemofiltration film（8）Lower section is provided with lower blotting paper（9）.
A kind of 2. detection plate based on the quick bracket for blood grouping of solid phase method according to claim 1, it is characterised in that the list
Resist for anti-A, anti-B, anti-D, the antibody groove（2）For three, in three antibody grooves（2）Inside be respectively arranged with anti-A, anti-B,
Anti- D, three antibody grooves（2）Pass through upper blotting paper respectively（7）With sample slot（5）Connection；
The gold mark erythrocyte membrane antigen is gold mark A films Ag, gold mark B films Ag, gold mark C films Ag, the gold mark groove（3）For three,
In three gold mark grooves（3）Inside it is respectively arranged with gold mark A films Ag, gold mark B films Ag, gold mark C films Ag, three gold mark grooves（3）Point
Do not pass through lower blotting paper（9）With sample slot（5）It is connected.
3. a kind of detection plate based on the quick bracket for blood grouping of solid phase method according to claim 1 or 2, it is characterised in that in institute
State antibody groove（2）Groove is marked with gold（3）Inside it is both provided with quick-fried pearl（4）, the monoclonal antibody is arranged at quick-fried with gold mark erythrocyte membrane antigen
Pearl（4）It is interior.
4. a kind of detection plate based on the quick bracket for blood grouping of solid phase method according to claim 1, it is characterised in that on described
Blotting paper（7）One end is arranged on antibody groove（2）Interior, the other end is arranged on sample slot（5）Sample slot is crossed in inside（5）；
The lower blotting paper（9）One end is arranged on gold mark groove（3）Interior, the other end is arranged on sample slot（5）Sample is crossed in inside
Groove（5）.
5. according to a kind of detection plate based on the quick bracket for blood grouping of solid phase method of claim 1-4 any one of them, its feature exists
In the upper blotting paper（7）It is arranged at hemofiltration film（8）Top.
A kind of 6. bracket for blood grouping method, it is characterised in that it utilizes claim 1-5 any one of them detection plates, including as follows
Step：
Step 1：By antibody groove（2）Groove is marked with gold（3）Interior quick-fried pearl（4）Rack, make quick-fried pearl（4）Interior reagent outflow；
Step 2：In sample slot（5）Interior addition whole blood sample, in upper blotting paper（7）Under the action of whole blood to antibody groove（2）Move
Dynamic, red blood cell is reacted with monoclonal antibody, carries out positive definite form；In hemofiltration film（8）Under the action of filter out the blood plasma after red blood cell in lower water suction
Paper（9）Under the action of to gold mark groove（3）Mobile, blood plasma and gold mark erythrocyte membrane antigen reaction, carry out reverse type；
Step 3：Reaction result in observation of steps two, according to positive reverse type aggegation result judgement blood group.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711391282.2A CN107907696B (en) | 2017-12-21 | 2017-12-21 | Detection plate and identification method based on solid-phase method for rapid blood grouping |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711391282.2A CN107907696B (en) | 2017-12-21 | 2017-12-21 | Detection plate and identification method based on solid-phase method for rapid blood grouping |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN107907696A true CN107907696A (en) | 2018-04-13 |
| CN107907696B CN107907696B (en) | 2024-04-12 |
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711391282.2A Active CN107907696B (en) | 2017-12-21 | 2017-12-21 | Detection plate and identification method based on solid-phase method for rapid blood grouping |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN110174511A (en) * | 2019-05-09 | 2019-08-27 | 昆明市儿童医院 | ABO Hemolysis quick detection test paper |
| CN113634295A (en) * | 2021-09-14 | 2021-11-12 | 南京岚煜生物科技有限公司 | Microfluidic blood type detection chip |
| CN114397464A (en) * | 2022-03-24 | 2022-04-26 | 天津德祥生物技术有限公司 | Coupling compound of erythrocyte membrane fragments and carrier, coupling method and application thereof |
| CN114660305A (en) * | 2022-03-31 | 2022-06-24 | 中国人民解放军军事科学院军事医学研究院 | Reagent for rapidly detecting blood type antibody titer, preparation method, test strip and application |
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| CN110174511A (en) * | 2019-05-09 | 2019-08-27 | 昆明市儿童医院 | ABO Hemolysis quick detection test paper |
| CN113634295A (en) * | 2021-09-14 | 2021-11-12 | 南京岚煜生物科技有限公司 | Microfluidic blood type detection chip |
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| CN114397464B (en) * | 2022-03-24 | 2022-06-10 | 天津德祥生物技术有限公司 | Coupling compound of erythrocyte membrane fragments and carrier, coupling method and application thereof |
| CN114660305A (en) * | 2022-03-31 | 2022-06-24 | 中国人民解放军军事科学院军事医学研究院 | Reagent for rapidly detecting blood type antibody titer, preparation method, test strip and application |
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| Publication number | Publication date |
|---|---|
| CN107907696B (en) | 2024-04-12 |
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