KR20170006981A - Blood typing device and method of blood typing using the same - Google Patents
Blood typing device and method of blood typing using the same Download PDFInfo
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- KR20170006981A KR20170006981A KR1020150098503A KR20150098503A KR20170006981A KR 20170006981 A KR20170006981 A KR 20170006981A KR 1020150098503 A KR1020150098503 A KR 1020150098503A KR 20150098503 A KR20150098503 A KR 20150098503A KR 20170006981 A KR20170006981 A KR 20170006981A
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/80—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood groups or blood types or red blood cells
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/54306—Solid-phase reaction mechanisms
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
- G01N33/544—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being organic
- G01N33/545—Synthetic resin
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Abstract
A blood type testing apparatus according to an embodiment of the present invention includes a support plate and a plurality of regions provided on one side of the support plate and provided with different antibodies. When the blood of the subject is provided to the above areas, different characters are displayed by the attachment reaction of the antibody to the antigen in the blood.
Description
More particularly, the present invention relates to a blood type test apparatus for immediately determining a blood type of a subject by simply contacting blood on a site without any separate equipment or sample, and a blood type test apparatus for testing blood type And a blood type test method using the same.
Human blood types can be divided into various categories such as ABO type, Rh type, MNS type, Kell type, Lewis type, Duffy type and Kidd type. Clinically, the ABO and Rh type are the most important and are the reference material for transfusion. The ABO and Rh blood types are classified according to the presence or absence of the A, B and Rh 0 (D) antigens present in the red blood cells, and those having different blood types have antibodies against other antigens and incompatible blood is injected An aggregation reaction occurs on the target blood cells. The blood type test is based on the principle of agglutination reaction between these unsophisticated blood. That is, the blood reacting to the anti-A antigen serum is judged to be A type, the blood reacting to the anti-B antigen serum is type B, and the blood reacting to both are judged to be the AB type. It is judged to be type O without antigen. Similarly, the blood that reacts to the anti-D antigen serum is identified as Rh +, and the non-reactive blood as Rh-like.
The present invention provides a blood typing apparatus and a blood typing method using the blood typing apparatus capable of instantaneously discriminating a blood type without involving separate education, specific props and reaction vessels.
A blood type testing apparatus according to an embodiment of the present invention includes a support plate and a plurality of regions provided on one side of the support plate and provided with different antibodies. When the blood of the subject is provided to the above areas, different characters are displayed by the attachment reaction of the antibody to the antigen in the blood. Unlike the coagulation reaction in the free liquid state, blood cells are attached to immobilized antibodies, which means information display by blood.
In one embodiment of the present invention, the regions comprise a first region coated with a first antibody and having an A-shape when viewed in plan, a first region coated with a second antibody different from the first antibody, and having a B- A second region, and a third region having an O-shape applied with an antibody that causes an adhesion reaction to all blood.
In one embodiment of the present invention, the support plate may be formed of a membrane made of polymer, glass, or silicone, or a filter paper, and the polymer may be a polymer selected from the group consisting of nitrocellulose, polyethylene, polypropylene, polyvinylidenedifluoride, Polydimethylsiloxane.
In one embodiment of the present invention, the support plate may be provided in a region other than where the regions are provided, and may further include a blocking portion for preventing nonspecific hemocyte adsorption. The blocking moiety may include a protein such as albumin or casein or a peptide that degrades the protein.
In one embodiment of the present invention, the support plate may further include a protection film provided to cover the support plate.
The antibody may be an IgA, IgD, IgE, IgM, IgG, a subunit of these or a specific domain thereof, or a mixture thereof, derived from an antibody library or derived from a peptide library It is possible.
The blood type test method according to an embodiment of the present invention includes the steps of providing a support plate having a plurality of regions, applying each of the regions of the support plate to react with different antigens to form a reaction portion, And a step of identifying the blood type indicated by a letter according to an adhesion reaction in each reaction part of the blood.
In one embodiment of the present invention, the support plate comprises a first region coated with the first antibody and having an A-shape when viewed in a plan view, a first region coated with the second antibody different from the first antibody, and a B- A second region, and a third region that is coated with a third antibody that reacts with all of the blood and has an O shape.
In one embodiment of the present invention, the blood type testing apparatus may further include a protective film provided on the support plate so as to cover the support plate, wherein the protective film is provided before the step of providing the blood of the subject to the reaction section Can be removed.
According to the object of the present invention, the blood cell itself displays its own blood type by the reaction with the specific antibody. Therefore, it is possible to immediately determine the blood type of the subject without waiting for a three-dimensional coagulation reaction by merely putting the blood of the subject in the examination apparatus, and a separate props such as a pipette are not required. In addition, a user who has not used any training or training related to the hemagglutination reaction can be included in the user, so that it can be widely applied to a situation in which a blood type test is required. It can be used as a means of providing immediate information in an emergency site, for example, by providing an uncomplicated, immediate manipulation of the blood type.
1 is an exploded perspective view of a blood type test apparatus according to an embodiment of the present invention.
FIG. 2A is a plan view showing the reaction unit of FIG. 1. FIG.
2B is a cross-sectional view taken along the line I-I 'in FIG. 2A.
3 is a conceptual diagram illustrating the principle of blood type testing of the blood type testing apparatus according to an embodiment of the present invention.
4 is a plan view showing the final result of the blood type testing apparatus when the blood type of the subject blood is of the A type.
Fig. 5 is a plan view showing the final result of the blood type testing apparatus when the blood type of the subject blood is type B. Fig.
Fig. 6 is a plan view showing the final result of the blood type testing apparatus when blood type of the subject is type O. Fig.
Fig. 7 is a plan view showing the final result of the blood type testing apparatus when the blood type of the subject blood is AB type.
DETAILED DESCRIPTION OF THE PREFERRED EMBODIMENTS Reference will now be made in detail to embodiments of the present invention, examples of which are illustrated in the accompanying drawings, wherein like reference numerals refer to the like elements throughout. However, these are only for illustrating the present invention, and the scope of the present invention is not limited by these examples.
1 is a perspective exploded view of a blood type testing apparatus according to an embodiment of the present invention.
FIG. 2A is a plan view showing the reaction unit of FIG. 1, and FIG. 2B is a cross-sectional view taken along the line I-I 'of FIG. 2A.
In one embodiment of the present invention, the blood type test apparatus utilizes an antigen-antibody reaction and can be used for various blood type tests. Here, the blood type testing apparatus can be applied not only to the ABO type of the blood type of human blood, but also to the blood type of another type, and also to the blood type of other animals.
Hereinafter, in one embodiment of the present invention, an ABO blood type test apparatus will be described as an example.
1, 2A, and 2B, the blood type testing apparatus according to an embodiment of the present invention includes a reaction unit RX for dropping and reacting blood of a subject, a protection film PRT1 for protecting the reaction unit RX , PRT2).
The reaction part RX is provided with the blood of the subject and the reaction of the provided blood is performed.
The reaction unit RX includes a support plate SP and a plurality of regions provided on one side of the support plate SP.
The support plate SP is a substrate on which a plurality of regions to be described later are provided. The support plate SP may be made of a membrane or a filter paper made of polymer, glass, silicon, or the like. When the support plate SP is made of a polymer, the polymer may be nitrocellulose, polyethylene, polypropylene, polyvinylidene difluoride, or polydimethylsiloxane. In addition, the support plate SP may include a microstructure fabricated in a top-down or bottom-up manner on an upper surface thereof.
The support plate SP may be provided in a different shape or in different sizes depending on the positions and sizes of the regions. For example, the support plate SP may be provided in a rectangular shape having a size of about 5 cm in width and about 4 cm in length. In an embodiment of the present invention, it has been shown that it is provided in a rectangular shape.
The regions are regions to which an antibody that reacts with the blood of the subject is provided, each having a different character shape. The character is provided as a simple one that can be grasped instantaneously at the moment when the examiner confirms with eyes. For example, if the blood type testing device according to an embodiment of the present invention is for testing an ABO blood type, the letter may include A, B, and O.
In one embodiment of the present invention, the regions are spaced apart from one another. Each region is provided with a different antibody.
According to one embodiment of the present invention, the regions include a first region RG1 to be displayed having an A shape, a second region RG2 having a B shape, and a third region RG3 having a C shape . The first region RG1, the second region RG2, and the third region RG3 are spaced apart from each other.
Different antibodies are provided in the first region RG1, the second region RG2, and the third region RG3, respectively. In one embodiment of the present invention, a first antibody is provided in the first region RG1, a second antibody is provided in the second region RG2, and a third antibody is provided in the third region RG3. The antibody may be set according to the type of blood to be determined. In an embodiment of the present invention, the third antibody may be an antibody that reacts with all blood cells.
Here, the first antibody may be an anti-A antibody and the second antibody may be an anti-B antibody. Accordingly, the anti-A antibody in the first region (RG1) may cause an adhesion reaction with the A red blood cells in the blood of the subject, and the anti-B antibody in the second region (RG2) may cause the adhesion reaction with the B red blood cells in the blood of the subject have. Also, the third region RG3 can be attached to all blood cells.
The first to third regions RG3 are set for examining the ABO blood group. When the blood type of another method is to be examined, the number of regions and the number of antibodies corresponding to the region may vary.
In one embodiment of the present invention, the antibody is capable of causing an antigen-antibody reaction with the blood of the subject, and is not particularly limited. For example, an antibody according to one embodiment of the present invention may be IgA, IgD, IgE, IgM, IgG, a sub-unit thereof or a specific domain thereof, or a mixture thereof. The antibody may also be a peptide fragment derived from an antibody library or derived from a peptide library. In addition, the antibody may be a monoclonal antibody or a polyclonal antibody, or a mixture thereof.
For example, the first to third antibodies may suffice to cause a specific adherence reaction to a specific blood type, and are not particularly limited. In particular, in the case of the third antibody, it can be selected from the above-mentioned antibodies as being attached to all blood.
The antibody may be derived from a mouse, a rat, a rabbit, a goat, a sheep, and the like. However, the present invention is not limited thereto, and the content of the present invention is not limited by the producing animal of the antibody.
In one embodiment of the present invention, when the support plate SP is a nitrocellulose membrane, the concentration of the antibody per unit area (cm 2 ) may be 0.1 mg or less. The amount of the antibody in each region can be determined by the amount of the blood to be examined and the characteristics of the support layer, and is not limited to the embodiment.
A blocking portion BL is provided on an upper surface of the support plate SP except for the first to third regions RG3. The blocking portion BL prevents nonspecific hemocyte adsorption, and the blocking portion BL may include a polymer protein such as albumin or casein. Further, the blocking portion BL may be a peptide mixture in which the polymer protein is degraded, and is not particularly limited as long as it minimizes nonspecific hemocyte deposition and adhesion. In addition, in an embodiment of the present invention, the blocking portion BL may be a SAM (self-assembled monolayer) film, and the blocking portion BL may be formed by a nonspecific adhesion inhibition method using such a chemical method .
The protective film may be provided on at least the upper surface of the reaction part RX so as to protect the reaction part RX. The protective film may be made of a non-hygroscopic material. The material of the protective film is selected from a polymer such as plastic or vinyl, or a material capable of blocking moisture loss such as glass.
In one embodiment of the present invention, the protective layer may include an upper protective layer PRT1 covering the upper surface of the supporting plate SP and a lower protective layer PRT2 covering the lower surface of the supporting plate SP. The lower protective film PRT2 may be omitted if necessary.
The upper protective film PRT1 covers all the regions on the support plate SP. The activity of the antibody provided in each of the regions is maintained by the upper protective film PRT1. The upper protective film PRT1 is provided in the form of a release film, and then removed before the blood of the subject is supplied to the reaction part RX.
The blood type test method according to an embodiment of the present invention includes providing a support plate SP and applying and fixing antibodies reactive with different antigens to respective regions of the support plate SP to form a reaction unit RX , And supplying blood to the reaction section RX. This will be described in detail with reference to FIGS. 1, 2A and 2B.
According to one embodiment of the present invention, a reaction unit RX is first formed to produce the blood type test apparatus.
The reaction unit RX may be prepared by preparing a plurality of support plates SP in which a plurality of regions are set and forming corresponding antibodies in the respective regions of the support plate SP.
The support plate SP may be made of a membrane or a filter paper made of polymer, glass, silicon, or the like. An area to which a plurality of antibodies are to be applied is set in the support plate SP.
The antibody may be provided in various ways in each region. For example, in one embodiment of the present invention, the antibody may be provided in each region by means of spotting, stamping, soft lithography, or the like.
Attachment of the antibody can be carried out in various ways. For example, in one embodiment of the present invention, the antibody may be attached by non-covalent electrostatic adsorption, and may comprise protein A, protein G, protein A / G, Or the like may be used. Covalent bonds such as a disulfide bridge may also be used. In the present invention, the method of attaching the antibody is not particularly limited.
The antibody is provided sufficiently to display information of a character form attached to the blood of the subject, and is not particularly limited in terms of its concentration and amount. For example, the antibody may be contained in a medium such as saline at a concentration of 0.1 mg / ml to 1 mg / ml and may be provided in each region. The solution containing the antibody may be dried to such an extent that part of the medium remains to maintain the activity of the antibody. The amount of the antibody can be adjusted according to the material and the characteristics of the support plate SP.
A blocking portion BL may be formed in a region other than the region where the antibody is provided.
The blocking part BL is formed by covering each area of the support plate SP, immersing it in a solution of albumin or casein at a concentration of about 1% for 1 minute or more, and then washing with a washing solution to remove excess blocking solution . The washing solution may be PBS (phosphate-buffered saline), but is not limited thereto. After the blocking portions BL are formed, the respective regions may be exposed again.
Thereafter, a protective film is provided on the support plate SP. The protective layer includes an upper protective layer PRT1 provided on the upper surface of the support plate SP and a top protective layer PRT1 provided on the lower surface. The upper protective film PRT1 covers each region of the support plate SP. The lower protective film PRT2 covers the lower surface of the support plate SP and may be omitted if necessary.
The blood type test apparatus is completed by the above-described method. A method of examining the blood type using the blood type testing apparatus is as follows.
First, the upper protective film PRT1 provided on the reaction part RX is removed. The upper protective film PRT1 is provided in the form of a release film and can be easily removed from the reaction part RX.
Next, blood of the subject is dropped onto the upper surface of the reaction part RX. The blood of the subject may be provided to the extent that it covers the respective regions in the order of several milliliters, and the extra blood shakes off the blood type testing apparatus. According to an embodiment of the present invention, since the blocking portion BL is provided on the upper surface of the support plate SP, an excessive amount of blood can be effectively removed even if the blood is simply blown off.
The blood of the subject dropped into the reaction part RX shows different results depending on the blood type.
FIG. 3 is a conceptual diagram showing the principle of blood type testing of the blood type testing apparatus according to an embodiment of the present invention, and FIGS. 4 to 7 sequentially show a blood type testing apparatus according to blood type A, B, ≪ / RTI >
Hereinafter, a blood type audit method according to an embodiment of the present invention will be described with reference to FIG. 3 to FIG.
3 and 4, when the blood type of the subject blood is of the A type, the blood contains the A type antigen, and the A type antigen is bound to the anti-A antibody of the first region (RG1) Respectively. There is no anti-A antibody in the second region (RG2), so that no adhesion reaction occurs. Since the third region RG3 contains an antibody that binds to all blood and causes an adhesion reaction, the blood cells in the blood bind to the antibody to cause an adhesion reaction.
As a result, the blood type test apparatus displays an "AO" character as an end result. From the displayed character "AO ", the examiner can immediately know that the blood type of the subject is Type A. In addition, the final display character "AO " also means a blood type that can be transfused, and the examiner can immediately know from this that the blood of the subject can be transfused from the object having the A type and the O type blood type.
3 and 5, when the blood type of the subject is type B, the blood contains a type B antigen, and the type B antigen is combined with the anti-B antibody of the second region (RG2) Respectively. Since there is no anti-B antibody in the first region (RG1), an adhesion reaction does not occur. Since the third region RG3 contains an antibody that binds to all blood and causes an adhesion reaction, the blood cells in the blood bind to the antibody to cause an adhesion reaction.
As a result, the blood type testing apparatus displays a "BO" character as an end result. From the displayed character "BO ", the examiner can immediately know that the blood type of the subject is Type B. In addition, the final display character "BO" also means a blood type that can be transfused, and the examiner can immediately know from this that the blood of the subject can be transfused from a subject having blood type B and type O blood.
3 and 6, when the blood type of the subject's blood is type O, the blood does not contain all of the A type and B type antigens. In both of the first region RG1 and the second region RG2, The adhesion reaction does not occur. However, since the third region RG3 contains an antibody that binds to all the blood and causes an adhesion reaction, the blood cells in the blood bind to the antibody to cause an aggregation reaction.
As a result, the blood type testing apparatus displays an "O" character as an end result, and the examiner immediately recognizes that the blood type of the subject is O type and that the blood of the subject can be transfused from a subject having the O type blood type Can be grasped immediately.
3 and 7, when the blood type of the subject's blood is of the AB type, the blood contains the A type antigen and the B type antigen, and the A type antigen is the anti-A antibody of the first region (RG1) , The anti-B type antibody in the second region (RG2), and the anti-A antibody and the anti-B antibody in the third region (RG3). Since the third region RG3 contains an antibody that binds to all blood and causes an adhesion reaction, the blood cells in the blood bind to the antibody to cause an adhesion reaction. As a result, the blood type test apparatus displays an "ABO" character as an end result. The inspector can immediately know from the displayed character "ABO" that the subject's blood type is AB type. The final display character "ABO" also means a blood type that can be transfused. From this, the examiner can immediately recognize that the subject's blood can be transfused from a subject having A, B, AB and O blood types have.
As described above, the blood type test apparatus according to an embodiment of the present invention is small in volume, simple, easy to manufacture, and is not a three-dimensional coagulation reaction, but performs immediate reading by attaching blood cells. It is possible to immediately determine the blood type of the subject within a very short time simply by being buried in the test apparatus. In addition, a separate props such as a pipette are not required to determine the blood type. In addition, a user who has never used any training or training related to hemocyte aggregation reaction can be included in the user, so that the present invention can be widely applied to a situation in which a blood type test is required. It can be used as a means of providing immediate information in an emergency site, for example, by providing an uncomplicated, immediate manipulation of the blood type.
In the embodiment of the present invention, the blood type testing device for determining the ABO blood type and the testing method using the blood type testing device have been described, but the embodiment of the present invention is not limited thereto. It goes without saying that the present invention can be applied to other blood type testing devices and blood type testing methods that cause an aggregation reaction using an antigen-antibody reaction. For example, the blood that reacts with the anti-D antigen serum can be identified as Rh + type, and the unreacted blood type can be identified as Rh-type, and immediate blood type determination can be performed using the above-described principle.
Further, in another embodiment of the present invention, the ABO blood type test device and Rh type blood type test device can be separately manufactured, but the ABO blood type test device and Rh type blood type test device can be formed by using one support plate Of course.
While the present invention has been particularly shown and described with reference to exemplary embodiments thereof, it is to be understood that the invention is not limited to the disclosed exemplary embodiments, It belongs to the scope of right.
Claims (20)
A plurality of regions provided on one side of the support plate and provided with different antibodies,
And displays different characters by the blood cells as a result of the adhesion reaction of the antibody to the antigen in the blood when the blood of the subject is provided to the regions.
Said regions being coated with a first antibody and having a first region having an A-shape when viewed in plan, a second region having a second B-form coated with said first antibody and a second antibody, and a second region having a B- And a third region having a shape of O, to which the third antibody causing the reaction is applied.
Wherein the support plate comprises a membrane made of polymer, glass, or silicone, or filter paper.
Wherein the polymer is nitrocellulose, polyethylene, polypropylene, polyvinylidene difluoride, or polydimethylsiloxane.
Wherein the support plate is provided in an area other than where the areas are provided and further comprises a blocking part for preventing nonspecific hemocyte adsorption.
Wherein the blocking portion comprises albumin or casein.
And a protective film provided on the top of the support plate to cover the support plate.
Wherein the antibody is an IgA, IgD, IgE, IgM, IgG, a subunit thereof or a specific domain thereof, or a mixture thereof.
Wherein the antibody is a peptide fragment derived from an antibody library or derived from a peptide library.
Applying reactive sites to the regions of the support plate to form reactive sites;
Providing the blood of the subject to the reaction part; And
And confirming a blood type indicated by a letter according to an adhesion reaction in each reaction part of the blood.
The support plate comprises a first region coated with the first antibody and having an A-shape when viewed in plan, a second region coated with the first and second antibodies and having a B-shape when viewed in a plan view, And a third region coated with a third antibody causing the reaction and having an O shape.
Wherein the support plate comprises a polymer, glass, silicone, or filter paper.
Wherein the polymer is nitrocellulose, polyethylene, polypropylene, polyvinylidene difluoride, or polydimethylsiloxane.
Wherein the support plate is provided in an area other than where the areas are provided and further comprises a blocking part to prevent nonspecific hemocyte adsorption.
Wherein the blocking portion comprises albumin or casein.
The method of claim 1, further comprising a protective layer provided on the support plate to cover the support plate, wherein the protective layer is removed prior to providing the blood of the examinee to the reaction unit.
Wherein each antibody reacts with one of human A, B, and Rh antigens.
Wherein said antibody is IgA, IgD, IgE, IgM, IgG, a subunit thereof or a specific domain thereof, or a mixture thereof.
Wherein said antibody is a peptide fragment derived from an antibody library or derived from a peptide library.
Wherein said antibody is a single cell, a multicellular cell, or a mixture thereof.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR1020150098503A KR20170006981A (en) | 2015-07-10 | 2015-07-10 | Blood typing device and method of blood typing using the same |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| KR1020150098503A KR20170006981A (en) | 2015-07-10 | 2015-07-10 | Blood typing device and method of blood typing using the same |
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| Publication Number | Publication Date |
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| KR20170006981A true KR20170006981A (en) | 2017-01-18 |
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| Application Number | Title | Priority Date | Filing Date |
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| KR1020150098503A KR20170006981A (en) | 2015-07-10 | 2015-07-10 | Blood typing device and method of blood typing using the same |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107907696A (en) * | 2017-12-21 | 2018-04-13 | 中国人民解放军白求恩国际和平医院 | A kind of detection plate and identification method based on the quick bracket for blood grouping of solid phase method |
| CN108499368A (en) * | 2018-01-31 | 2018-09-07 | 汕头伊能膜业有限公司 | A kind of nitrocellulose microporous barrier and preparation method thereof with paper gasket pad |
| KR20180137130A (en) * | 2017-06-16 | 2018-12-27 | 고려대학교 산학협력단 | Blood type detection kit |
| US20210373007A1 (en) * | 2018-04-06 | 2021-12-02 | University Of Rochester | Abo blood group point-of-care testing |
| US11899026B2 (en) | 2019-10-07 | 2024-02-13 | University of Rochester, Rochester Institute of Technology | ABO blood group point-of-care chip testing |
-
2015
- 2015-07-10 KR KR1020150098503A patent/KR20170006981A/en unknown
Cited By (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| KR20180137130A (en) * | 2017-06-16 | 2018-12-27 | 고려대학교 산학협력단 | Blood type detection kit |
| CN107907696A (en) * | 2017-12-21 | 2018-04-13 | 中国人民解放军白求恩国际和平医院 | A kind of detection plate and identification method based on the quick bracket for blood grouping of solid phase method |
| CN107907696B (en) * | 2017-12-21 | 2024-04-12 | 中国人民解放军白求恩国际和平医院 | Detection plate and identification method based on solid-phase method for rapid blood grouping |
| CN108499368A (en) * | 2018-01-31 | 2018-09-07 | 汕头伊能膜业有限公司 | A kind of nitrocellulose microporous barrier and preparation method thereof with paper gasket pad |
| US20210373007A1 (en) * | 2018-04-06 | 2021-12-02 | University Of Rochester | Abo blood group point-of-care testing |
| US11940445B2 (en) * | 2018-04-06 | 2024-03-26 | University Of Rochester | ABO blood group point-of-care testing |
| US11899026B2 (en) | 2019-10-07 | 2024-02-13 | University of Rochester, Rochester Institute of Technology | ABO blood group point-of-care chip testing |
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