CN107904227A - In vitro culture electric stimulation and application thereof - Google Patents
In vitro culture electric stimulation and application thereof Download PDFInfo
- Publication number
- CN107904227A CN107904227A CN201710954002.8A CN201710954002A CN107904227A CN 107904227 A CN107904227 A CN 107904227A CN 201710954002 A CN201710954002 A CN 201710954002A CN 107904227 A CN107904227 A CN 107904227A
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- Prior art keywords
- tissue
- culture
- cell
- unit
- electrical stimulation
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N13/00—Treatment of microorganisms or enzymes with electrical or wave energy, e.g. magnetism, sonic waves
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M21/00—Bioreactors or fermenters specially adapted for specific uses
- C12M21/08—Bioreactors or fermenters specially adapted for specific uses for producing artificial tissue or for ex-vivo cultivation of tissue
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
- C12M35/02—Electrical or electromagnetic means, e.g. for electroporation or for cell fusion
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0618—Cells of the nervous system
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0618—Cells of the nervous system
- C12N5/0619—Neurons
Abstract
The present invention provides a kind of cell or tissue culture electric stimulation and application thereof, the system comprises control module, electrical stimulation unit, some stimulating electrodes, several cell or tissue culture units, the control module connects electrical stimulation unit, the electrical stimulation unit uses artificial cochlea, the culture unit is used to cultivate cell or tissue, the electrical stimulation unit provides electro photoluminescence for each culture unit, the system can be used for cultivating cell or tissue, flexible and convenient to use, the control for stimulus signal is more flexible.The equipment used at the same time is simpler, will not be influenced be subject to other equipment.In particular researcher studies stimulation of the artificial cochlea to inner ear tissue or Spiral ganglion and provides great convenience, greatly improves Efficiency.
Description
Technical field
The present invention relates to a kind of in vitro culture electric stimulation and application thereof.
Background technology
Electro physiology treatment means are more and more introduced in medical field, various nerve stimulators continue to develop, such as heart
Pacemaker, artificial cochlea, sacral nerve stimulator, artificial eye etc..All it is to complete related invalid treatment by electro photoluminescence.To subtract
Hypopathia pain or certain dysfunction are improved.
The application of artificial cochlea implantation history more than 40 years existing, by auditory electro-stimulation in rats, restarts auditory effect, helps
Severe deafness patient recovers hearing.Its speech audition effect has met the needs of most of patients, and technical principle has tended to be ripe.Grind
Study carefully the Neurobiology Behavioral change of nerve cell or other cells under electro photoluminescence, there is highly important prospect and meaning.
The electrical stimulation method used when at present, for nerve cell or tissue cultures, or it is more traditional, all it is using general
Logical electrode stimulates cell or tissue as signal source.But above-mentioned cultural method has the following disadvantages：Normal signal source can not
Flexibly set, stimulus signal underaction, such as stimulation amplitude, stimulate width, frequency of stimulation, stimulate alternately polarity；Equipment need to be used
It is more；Without electrical isolation, disturbed by other equipment.
The content of the invention
In view of the foregoing deficiencies of prior art, it is an object of the invention to provide a kind of in vitro culture electric stimulation
And application thereof, the device of electro photoluminescence is inconvenient for use during for solving to cultivate cell or tissue in the prior art, underaction asks
Topic.
In order to achieve the above objects and other related objects, the present invention provides artificial cochlea and provides electro photoluminescence for cell or tissue
Purposes.
Further, the cell or tissue refers to nerve cell or tissue.
Further, the cell or tissue refers to ear cells or tissue.
Further, the nerve cell or tissue refer to Spiral ganglion or spiral ganglion tissue.
Another aspect provides a kind of in vitro culture electric stimulation, the system comprises control module, electricity
Stimulating unit, some stimulating electrodes, several culture units, the control module connect electrical stimulation unit, the electro photoluminescence list
Member uses artificial cochlea, and the electrical stimulation unit connects stimulating electrode, and each stimulating electrode connects a culture unit, institute
State culture unit to be used to cultivate cell or tissue, the electrical stimulation unit is to provide electricity by stimulating electrode for each unit of cultivating
Stimulate.
The artificial cochlea is the prior art, and those skilled in the art can be purchased in market.Artificial cochlea wraps in the prior art
Include decoding stimulator.
Preferably, the artificial cochlea includes decoding stimulator, the decoding stimulator connection stimulating electrode.
Further, the control module includes current control unit, for controlling the current strength of each stimulating electrode
And the time.
Further, the culture unit is chamber culture slide.Chamber culture glass slide is the prior art, can be with city
Purchase.
Further, the number of the stimulating electrode is 6~10.
Further, two stimulating electrodes are set to connect hole on the culture unit.Respectively as the electricity of a stimulating electrode
Stream outflow connector and electric current flow into connector.
Another aspect provides above-mentioned in vitro culture device to be used for the offer electro photoluminescence of cell or tissue
Purposes.
The cell and tissue can be various cells and various tissues, unrestricted.Especially it is cochlear tissue, spiral
Neuronal cell or tissue.
As described above, in vitro culture electric stimulation of the present invention and application thereof, has the advantages that：
The in vitro culture electric stimulation can be flexible and convenient to use, and the control for stimulus signal is more flexible.Together
The equipment of Shi Caiyong is simpler, will not be influenced be subject to other equipment.In particular it is thin to nerve to study artificial cochlea by researcher
The stimulation of born of the same parents or tissue provides great convenience, greatly improves Efficiency.The present invention is due to make use of artificial cochlea
Multichannel stimulatory function technology, can stimulate multiple culture units at the same time, and the electric charge for providing different mode respectively as needed is put down
Weighing apparatus formula electric pulse stimulation.
Brief description of the drawings
Fig. 1 is shown as in vitro culture electric stimulation structure diagram.
Fig. 2 is shown as electric current stimulus intensity and frequency diagram in the present embodiment.
Component label instructions
1 control module
2 adapters
3 radio-frequency sending coils
4 receiving coils
5 decoding stimulators
6 stimulating electrodes
7 culture units
Embodiment
Embodiments of the present invention are illustrated by particular specific embodiment below, those skilled in the art can be by this explanation
Content disclosed by book understands other advantages and effect of the present invention easily.
Please refer to Fig.1 and 2.It should be clear that structure, ratio, size depicted in this specification institute accompanying drawings etc., only to
The revealed content of specification is closed, so that those skilled in the art understands and reads, being not limited to the present invention can implement
Qualifications, therefore do not have technical essential meaning, the modification of any structure, the change of proportionate relationship or the adjustment of size,
In the case where not influencing the effect of present invention can be generated and the purpose that can reach, should all still fall in disclosed technology
In the range of Rong get Neng is covered.It is meanwhile cited such as " on ", " under ", "left", "right", " centre " and " one " in this specification
Deng term, be merely convenient to understanding for narration, and be not used to limit the enforceable scope of the present invention, the change of its relativeness
Or adjustment, in the case where changing technology contents without essence, when being also considered as the enforceable category of the present invention.
As shown in Figure 1, the present invention provides a kind of in vitro culture electric stimulation, the system comprises control module 1, electricity thorn
Swash unit, 8 stimulating electrodes, 6,8 culture units 7, the control module connects electrical stimulation unit, and the electrical stimulation unit is adopted
Manually cochlea, electrical stimulation unit connection stimulating electrode, each stimulating electrode connect a culture unit, the culture unit 7
For cultivating cell or tissue, each stimulating electrode provides electro photoluminescence for each culture unit.
The control module can use computer in the present embodiment, wherein control program is loaded with, for controlling manually
Cochlea.In preferred embodiment, the control module includes current control unit, for controlling the electric current of each stimulating electrode
Intensity and time.
The present apparatus completes information by RF coupling and energy transmits, and the electrical isolation of RF coupling is advantageous to this reality
Test research.Artificial cochlea is provided by Lishengte Medical Science ＆ Tech. Co., Ltd..
Generally, the artificial cochlea includes adapter 2 and radio-frequency sending coil 3, take-up circle 4 and decoding stimulator 5.
Adapter 2 can connect computer by USB interface with data cable, and by adapter 2, stimulation electricity can be adjusted flexibly
Stream amplitude, the adapter connect radio-frequency sending coil 3, and radio-frequency sending coil 3 is used for passing through RF radio-frequency communications, to implant solution
Code device 5 sends boost pulse information, and such as stimulation amplitude stimulates width, frequency of stimulation, and the receiving coil 4 is used to receive radio frequency
The electric signal that transmitting coil 3 is sent, and then decoding stimulator 5 is sent a signal to, the decoding stimulator, which connects 8, stimulates electricity
Pole, each stimulating electrode connects a culture unit, and provides electro photoluminescence for the cell or tissue in each culture unit.Decoding
The main function of stimulator 5 is to receive radio-frequency information, is decoded, and exports the boost pulse of corresponding parameter.
The present invention can disposably connect multiple culture units, and noiseless from each other, relative to making in the prior art
Forming interference when being stimulated with multiple power supplys from each other has obvious advantage.
The present invention can provide difference due to make use of artificial cochlea's multichannel stimulatory function for each culture unit
The charge balance equation electric pulse stimulation of pattern.
In the present embodiment, the culture module is chamber culture slide.Two are set in the culture module stimulates electricity
Pole connects hole, respectively as the electric current outflow connector and electric current inflow connector of a stimulating electrode.Therefore, the electric current of stimulating electrode can
By cell culture fluid, then to stimulate cell or tissue.
In the present embodiment, what is cultivated on the chamber culture slide can be cochlear tissue or Spiral ganglion.This reality
Physiological reaction of the example particularly suitable for research ear cells under charge balance equation electric pulse stimulation is applied, to study human body inner ear
The physilogical characteristics of cell construct a very ingenious model, also the development for artificial cochlea's technology continue research provide it is important
Valuable information.
Certainly, in other embodiments, it can be used for placing other cell or tissues on chamber culture slide, be used for
Study physiological reaction of the cell or tissue under electro photoluminescence.
In other embodiments, the number of the stimulating electrode is 6~10, therefore, can give 6~10 culture units
Electro photoluminescence is provided, this is also to set as needed.
Referring to Fig. 2, in the present embodiment, one of boost pulse can be set to form a complete list by PH1 and PH2
Member.Frequency：250-12000Hz；Pulsewidth W：32-400us；Stimulation amplitude A：18-1800uA.PH1 and PH2 width, amplitude are complete
Symmetrically.Stimulate width and amplitude adjustable.Stimulus sequence is made of persistently multiple units, frequency-adjustable.Relative in the prior art
The width and amplitude that electric current stimulates all are fixed to have obvious advantage.
So the present invention effectively overcomes various shortcoming of the prior art and has high industrial utilization.
The above-described embodiments merely illustrate the principles and effects of the present invention, not for the limitation present invention.It is any ripe
Know the personage of this technology all can carry out modifications and changes under the spirit and scope without prejudice to the present invention to above-described embodiment.Cause
This, those of ordinary skill in the art is complete without departing from disclosed spirit and institute under technological thought such as
Into all equivalent modifications or change, should by the present invention claim be covered.
Claims (10)
1. artificial cochlea is used for the purposes that electro photoluminescence is provided for cell or tissue.
2. purposes according to claim 1, it is characterised in that：The cell or tissue refers to nerve cell or tissue.
3. purposes according to claim 1, it is characterised in that：The cell or tissue refers to ear cells or tissue.
4. a kind of in vitro culture electric stimulation, the system comprises control module (1), electrical stimulation unit, some stimulating electrodes
(6), several culture units (7), the control module (1) connect electrical stimulation unit, and the electrical stimulation unit uses artitificial ear
Snail, the electrical stimulation unit connection stimulating electrode (6), each stimulating electrode (6) connect a culture unit, the culture
Unit (7) is used to cultivate cell or tissue, and the electrical stimulation unit provides electricity thorn by stimulating electrode (6) for each culture unit
Swash.
5. in vitro culture electric stimulation according to claim 4, it is characterised in that：The control module includes electric current control
Unit processed, for controlling current strength and the time of each stimulating electrode.
6. in vitro culture electric stimulation according to claim 4, it is characterised in that：The culture unit is chamber culture
Slide.
7. in vitro culture electric stimulation according to claim 4, it is characterised in that：The artificial cochlea includes decoding and pierces
Swash device (5), the decoding stimulator connection stimulating electrode (6).
8. in vitro culture electric stimulation according to claim 4, it is characterised in that：The number of the stimulating electrode (6)
For 6~10.
9. as claim 4~8 any one of them in vitro culture electric stimulation is used for providing electro photoluminescence to cell, tissue
Purposes.
10. purposes according to claim 9, it is characterised in that the cell or tissue refers to nerve cell or tissue.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710954002.8A CN107904227A (en) | 2017-10-13 | 2017-10-13 | In vitro culture electric stimulation and application thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201710954002.8A CN107904227A (en) | 2017-10-13 | 2017-10-13 | In vitro culture electric stimulation and application thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN107904227A true CN107904227A (en) | 2018-04-13 |
Family
ID=61840614
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201710954002.8A Pending CN107904227A (en) | 2017-10-13 | 2017-10-13 | In vitro culture electric stimulation and application thereof |
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| Country | Link |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2774651A1 (en) * | 2011-10-31 | 2014-09-10 | OG Giken Co., Ltd. | Electrostimulation device and electrostimulation device system |
| CN104147695A (en) * | 2014-08-19 | 2014-11-19 | 浙江诺尔康神经电子科技股份有限公司 | Animal experiment auditory nerve stimulation electrode and device and manufacture method thereof |
| CN104974937A (en) * | 2015-08-11 | 2015-10-14 | 东南大学 | Cell electrophotoluminescene loading device |
-
2017
- 2017-10-13 CN CN201710954002.8A patent/CN107904227A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP2774651A1 (en) * | 2011-10-31 | 2014-09-10 | OG Giken Co., Ltd. | Electrostimulation device and electrostimulation device system |
| CN104147695A (en) * | 2014-08-19 | 2014-11-19 | 浙江诺尔康神经电子科技股份有限公司 | Animal experiment auditory nerve stimulation electrode and device and manufacture method thereof |
| CN104974937A (en) * | 2015-08-11 | 2015-10-14 | 东南大学 | Cell electrophotoluminescene loading device |
Non-Patent Citations (1)
| Title |
|---|
| 王宇 等: "电诱发听性脑干反应在人工耳蜗植入中的应用进展", 《中华耳科学杂志》 * |
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Application publication date: 20180413 |
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