CN107899058A - A kind of preparation method of bacteria cellulose-base anti-biotic material - Google Patents
A kind of preparation method of bacteria cellulose-base anti-biotic material Download PDFInfo
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- CN107899058A CN107899058A CN201711183628.XA CN201711183628A CN107899058A CN 107899058 A CN107899058 A CN 107899058A CN 201711183628 A CN201711183628 A CN 201711183628A CN 107899058 A CN107899058 A CN 107899058A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/18—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons containing inorganic materials
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/22—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons containing macromolecular materials
- A61L15/28—Polysaccharides or their derivatives
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L15/00—Chemical aspects of, or use of materials for, bandages, dressings or absorbent pads
- A61L15/16—Bandages, dressings or absorbent pads for physiological fluids such as urine or blood, e.g. sanitary towels, tampons
- A61L15/42—Use of materials characterised by their function or physical properties
- A61L15/46—Deodorants or malodour counteractants, e.g. to inhibit the formation of ammonia or bacteria
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/10—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices containing or releasing inorganic materials
- A61L2300/102—Metals or metal compounds, e.g. salts such as bicarbonates, carbonates, oxides, zeolites, silicates
- A61L2300/104—Silver, e.g. silver sulfadiazine
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2300/00—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices
- A61L2300/40—Biologically active materials used in bandages, wound dressings, absorbent pads or medical devices characterised by a specific therapeutic activity or mode of action
- A61L2300/404—Biocides, antimicrobial agents, antiseptic agents
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Abstract
The present invention discloses a kind of preparation method of bacteria cellulose-base anti-biotic material, is related to anti-biotic material technical field；Using bacteria cellulose as matrix, silver nitrate is selected as load silver plating solution, alkaline environment is provided in ethylenediamine, can be silver ion reduction into silver-colored simple substance, and trifluoroacetic acid has catalytic action, catalysis generation silver ion end compound, beneficial to silver ion reduction into silver-colored simple substance, the silver ion in load silver plating solution is thoroughly reduced into Argent grain through sodium potassium tartrate tetrahydrate tetrahydrate solution again, and load on the matrix of bacteria cellulose, obtain bacteria cellulose-base anti-biotic material；The method of the present invention is using biomass bacteria cellulose as raw material, abundant raw material, application of the natural biomass resource in anti-biotic material field is widened, and at normal temperatures, it can be realized as the load of the stable and uniform a large amount of nano-Ag particles in short time, Ag-carrying bacterial cellulose anti-biotic material is formed, beneficial to energy-saving consumption-reducing.
Description
Technical field
The present invention discloses a kind of preparation method of anti-biotic material, is related to anti-biotic material technical field, specifically a kind of
The preparation method of bacteria cellulose-base anti-biotic material.
Background technology
Nano silver, which has, produces unique bactericidal mechanism, can be directly entered in thalline, can directly be killed rapidly in low concentration
Dead pathogenic bacteria, make bacterium lose fertility, can not produce drug resistance.In addition, nano-Ag particles have superpower activity, to compared with
The tissue infection of depths has good bactericidal effect；There is layer protecting film outside nano silver, nano-Ag particles can be avoided to congeal into
Bulk, makes it gradually to be discharged in sterilization process, durable antibacterial effect.In order to make full use of the good characteristic of nano silver, subtract
Its few negative effect, can prepare composite medical jointly by nano silver and other materials such as gelatin, chitosan, bacteria cellulose etc.
Material is standby.
And bacteria cellulose is a kind of important natural macromolecular material, because wide with good biocompatibility
The general every field applied to organizational project.Since bacteria cellulose material has good mechanical property and excellent water conservation energy
Power, its application in Wound dressing are extremely concerned.In clinical process, surface wound is often accompanied by infection conditions, and bacterium is fine
Ability of the dimension element itself without antibacterial bacteriostatic, it is difficult to large-area burn wound, chronic wound surface in refractory to treatment for high infection risk etc..
And the feature Wound dressing for utilizing bacteria cellulose and nano silver to prepare then has strong antibacterial action, good biocompatibility, low
Poison, do not produce the features such as drug resistance, can effectively improve therapeutic effect, shortens wound healing time, is current biological medicine neck
The Hot Contents of domain research.
A kind of selectivity load antibiotic property of bacteria cellulose of the Introduction To Cn Patent of Publication No. CN102344496A
Nano silver method.The bacteria cellulose after freeze-drying is placed among nitroxyl/enzyme system solution first, adds oxidant
Reaction, then be placed in Diamines substance solution, heating reaction, is then immersed in silver nitrate solution, silver ion is sent out with amino
Raw chelation, obtains the bacteria cellulose containing selective load silver ion.Or by the thin of above-mentioned selective load silver ion
Fungin reduces to obtain the bacteria cellulose of selective loading nano silvery particle by high pressure.The invention can receive antibiotic property
Meter Yin is carried on bacteria cellulose, be firmly combined with it is not easily to fall off, but carry silver before bacteria cellulose need to be freeze-dried, complicate
Operation sequence, is not easy to promote and apply.
The Chinese patent of Publication No. CN102212806A provides a kind of system of bacterial cellulose-nano silver composite material
Preparation Method.After bacterial cellulose wet-coating is pre-processed, it is placed in silver salt solution and impregnates, the bacterial fibers of silver ion will be adsorbed
Element is transferred in polyglycol solution, then ascorbic acid solution is added dropwise to this mixed system, is centrifuged, washs and is dried
Afterwards, bacterial cellulose-nano silver composite material is obtained.The present invention is under polyethylene glycol protection using ascorbic acid reduction silver ion
Method, while play polyethylene glycol stabilization and ascorbic acid reaction it is gentle, nontoxic the advantages that, formed particle diameter it is small, point
The uniform nano composite system of cloth.But bacterial cellulose wet-coating need to pass through alkali process and acetic acid is handled, add in pretreatment section
Chemical cost, also complicates preparation process.
Chinese patent（Publication No. CN101586309）Describe a kind of bacteria cellulose of in-situ compounding simple-substance nano silvery
The preparation method of film.It is primarily characterized in that there is unique three-dimensional manometer network structure based on bacteria cellulose film, pass through silver
Ammonia solution with the chemical reagent containing aldehyde radical reaction in its network structure in-situ preparation silver simple substance nano particle.The invention improves
The shortcomings that argentiferous bacteria cellulose dry film brittleness is big, available for the biologic medical such as large area wound dressing, binder field, but
High-temperature process is needed in preparation process, and need to first prepare silver ammino solution, is unfavorable for preparation process simplification.
The Chinese patent of Publication No. CN102206355A provide a kind of silver nanoparticle-containing bacteria cellulose film and
Its preparation method.Nano silver grain is attached to the microfiber surface of bacteria cellulose film tridimensional network by the invention, is prepared
Step includes：First bacteria cellulose film is immersed at room temperature in silver salt solution soak 10 minutes -5 it is small when after take out, Ran Houyong
Distilled water clean bacteria cellulose film surface, afterwards using ultraviolet irradiation method irradiation 0.5-6 it is small when, obtain silver nanoparticle-containing
Bacterial cellulose wet-coating, most obtain the bacteria cellulose film of silver nanoparticle-containing through dehydration afterwards.The present invention is without chemistry
Go back original reagent participates in make to adhere to nano-Ag particles on bacteria cellulose film, and process conditions are green safe, are suitable for use as wound
Medical dressing, but since ultraviolet light penetration performance is weak, is only capable of the silver ion reduction of film surface into silver-colored simple substance, the element inside film
Silver still exists in the form of an ion, influences the clinical effectiveness of bacteria cellulose film.
Publication No. TW201640002（A）Taiwan Patent anti-biotic material is prepared with native cellulose and silver nano-grain,
First polyvinyl alcohol is dissolved in deionized water and prepares colloid envelope agent, adds silver nitrate and sodium borohydride in colloid envelope agent
In, silver ion reduction obtains silver-colored sodium rice particle colloidal sols into elemental silver, then by wood pulp cellulose and N-methylmorpholine-N- oxides
Mixed solution is added in colloidal sol, formed nano-Ag particles cellulose spinning fluid, then through dehydration, spinning, dry after prepare it is anti-
Fungin composite material.The present invention can prepare the higher antibacterial fiber material of intensity, but employ wood-cellulose and
N-methylmorpholine-N- oxides are base material, it is necessary to dissolve, spinning, and program is complicated, and the biocompatibility of base material with it is thin
Fungin compares poor, the necessity also further improved.
United States Patent (USP)（US2013211308 (A1)）Describe the bacteria cellulose anti-biotic material of a kind of nanometer of silver coating.
Bacteria cellulose is dispersed into fibrous suspension first, oxidant is added and aoxidizes scattered cellulose fibre, in oxidized fibre
The groups such as thio group, carbonyl, aldehyde radical or carboxyl are re-introduced on cellulose fiber skeleton and prepare functional fibre cellulose fiber, then by function
Cellulose fibre is immersed in albumen silver salt solution, fiber reinforcement surface is formed silver nano-grain, the thin of nanometer silver coating is made
Fungin antimicrobial composite material.Present invention introduces functional group to cure silver particles, is firmly combined with silver nano-grain, is not easy to take off
Fall, also beneficial to silver particles slow release effect.But decentralized processing has been carried out to bacteria cellulose in preparation process, destroy bacterium
The natural nano structure of cellulose, also needs further to probe into the subsequent affect of composite material.
And the present invention selects a small amount of silver nitrate as silver plating solution is carried using bacteria cellulose as carrier, in a small amount of second two
Under the action of amine and trifluoroacetic acid, silver-colored solution reduction is set successfully to load to bacterium into Argent grain through sodium potassium tartrate tetrahydrate tetrahydrate
On the matrix of cellulose, a kind of anti-biotic material of bacteria cellulose-base is obtained.Not only preparation process is simple, time-saving energy-saving, and
Efficient easy industrialization.
The content of the invention
The present invention provides a kind of preparation method of bacteria cellulose-base anti-biotic material, and used technical solution is：
A kind of preparation method of bacteria cellulose-base anti-biotic material, using bacteria cellulose as matrix, selects silver nitrate silver-colored as carrying
Plating solution, and under the action of ethylenediamine and trifluoroacetic acid, through sodium potassium tartrate tetrahydrate tetrahydrate solution make silver in load silver plating solution from
Son is reduced into Argent grain, and loads on the matrix of bacteria cellulose, obtains bacteria cellulose-base anti-biotic material.
The preparation method concretely comprises the following steps：
Prepare silver plating solution, including silver nitrate, ethylenediamine, trifluoroacetic acid；
Bacteria cellulose is taken to be impregnated into sodium potassium tartrate tetrahydrate tetrahydrate solution as matrix,
Silver plating solution is added in sodium potassium tartrate tetrahydrate tetrahydrate solution again, impregnates 0.5-5h,
Bacteria cellulose matrix is washed with deionized, obtains the bacteria cellulose-base anti-biotic material containing nano silver particles.
Silver nitrate that the preparation method, the silver plating solution of preparation, including weight fraction are 0.1-1%, volume fraction 1-
2% ethylenediamine, the trifluoroacetic acid that volume fraction is 0.05-2%.
The preparation method, takes bacteria cellulose to be impregnated into the potassium tartrate that mass concentration is 2%-20% as matrix
5-60min in sodium tetrahydrate solution.
The preparation method, tartaric acid is added to by the silver plating solution identical with sodium potassium tartrate tetrahydrate tetrahydrate liquor capacity
In potassium sodium tetrahydrate solution, 0.5-5h is impregnated.
A kind of bacteria cellulose-base anti-biotic material, is prepared via a method which：Using bacteria cellulose as matrix, nitric acid is selected
Silver makes load silver plating as load silver plating solution, and under the action of ethylenediamine and trifluoroacetic acid through sodium potassium tartrate tetrahydrate tetrahydrate solution
Silver ion reduction in liquid is loaded on the matrix of bacteria cellulose into Argent grain, obtains bacteria cellulose-base anti-biotic material.
The preparation of the anti-biotic material concretely comprises the following steps：
Prepare silver plating solution, including silver nitrate, ethylenediamine, trifluoroacetic acid；
Bacteria cellulose is taken to be impregnated into sodium potassium tartrate tetrahydrate tetrahydrate solution as matrix,
Silver plating solution is added in sodium potassium tartrate tetrahydrate tetrahydrate solution again, impregnates 0.5-5h,
Bacteria cellulose matrix is washed with deionized, obtains the bacteria cellulose-base anti-biotic material containing nano silver particles.
In the preparation process of the anti-biotic material：The silver plating solution of preparation, including weight fraction be 0.1-1% silver nitrate,
The trifluoroacetic acid that ethylenediamine that volume fraction is 1-2%, volume fraction are 0.05-2%.
In the preparation process of the anti-biotic material：Taking bacteria cellulose, it is 2%- to be impregnated into mass concentration as matrix
5-60min in 20% sodium potassium tartrate tetrahydrate tetrahydrate solution.
In the preparation process of the anti-biotic material：By the silver plating solution identical with sodium potassium tartrate tetrahydrate tetrahydrate liquor capacity
It is added in sodium potassium tartrate tetrahydrate tetrahydrate solution, impregnates 0.5-5h.
Beneficial effects of the present invention are：
The present invention provides a kind of preparation method of bacteria cellulose-base anti-biotic material, using bacteria cellulose as matrix, selects nitric acid
Silver provides alkaline environment as silver plating solution is carried, in ethylenediamine, can be silver ion reduction into silver-colored simple substance, and trifluoroacetic acid has catalysis
Effect, catalysis generation silver ion end compound, beneficial to silver ion reduction into silver-colored simple substance, then makes through sodium potassium tartrate tetrahydrate tetrahydrate solution
The silver ion carried in silver plating solution is thoroughly reduced into Argent grain, and loads on the matrix of bacteria cellulose, obtains bacteria cellulose
Base anti-biotic material；
The method of the present invention is using biomass bacteria cellulose as raw material, abundant raw material, has widened natural biomass resource in antibacterial material
Application in material field, and can be realized as the load of the stable and uniform a large amount of nano-Ag particles at normal temperatures, in the short time,
Ag-carrying bacterial cellulose anti-biotic material is formed, beneficial to energy-saving consumption-reducing.
Brief description of the drawings
Fig. 1 the method for the present invention flow diagrams；
Fig. 2 natural bacteria cellulose scanning electron microscope (SEM) photographs；
Ag-carrying bacterial cellulose scanning electron microscope (SEM) photograph in Fig. 3 present invention；
Single Ag-carrying bacterial cellulose scanning electron microscope (SEM) photograph in Fig. 4 present invention；
Fig. 5 Ag-carrying bacterial cellulose antibacterial film antibacterial effect photos of the present invention.
Embodiment
The present invention provides a kind of preparation method of bacteria cellulose-base anti-biotic material, and used technical solution is：
A kind of preparation method of bacteria cellulose-base anti-biotic material, using bacteria cellulose as matrix, selects silver nitrate silver-colored as carrying
Plating solution, and under the action of ethylenediamine and trifluoroacetic acid, through sodium potassium tartrate tetrahydrate tetrahydrate solution make silver in load silver plating solution from
Son is reduced into Argent grain, and loads on the matrix of bacteria cellulose, obtains bacteria cellulose-base anti-biotic material.
There is provided and a kind of corresponding bacteria cellulose-base anti-biotic material of the above method at the same time.
The present invention is made the following instructions with reference to embodiment：
Embodiment 1：
Silver plating solution, including 0.1% are made first（Wt%）Silver nitrate, 1%（V/V）Ethylenediamine and 0.05%（V/V）Trifluoroacetic acid；Again
The bacteria cellulose that dry weight is 0.05g is taken after deionized water is cleaned, to be dipped into the winestone that 20mL concentration is 2% as matrix
In sour potassium sodium tetrahydrate solution after 5min, 20mL silver plating solutions are taken to be added in sodium potassium tartrate tetrahydrate tetrahydrate solution, then impregnate
0.5h, reduction silver ion is nano-Ag particles, and is incorporated on bacteria cellulose matrix, is washed with deionized, and is made and contains
The native cellulose base anti-biotic material of nano silver particles.
Embodiment 2：
Silver plating solution, including 0.2% are made first（Wt%）Silver nitrate, 1%（V/V）Ethylenediamine and 0.2%（V/V）Trifluoroacetic acid；Again
The bacteria cellulose that dry weight is 0.5g is taken after deionized water is cleaned, to be dipped into the winestone that 50mL concentration is 5% as matrix
In sour potassium sodium tetrahydrate solution after 10min, 50mL silver plating solutions are taken to be added in sodium potassium tartrate tetrahydrate tetrahydrate solution, then impregnate
1h, reduction silver ion is nano-Ag particles, and is incorporated on bacteria cellulose matrix, is washed with deionized, and is made to contain and receives
The native cellulose base anti-biotic material of rice silver particles.
Embodiment 3：
Silver plating solution is first prepared, component includes 0.5%（Wt%）Silver nitrate, 1.2%（V/V）Ethylenediamine and 0.5%（V/V）Trifluoroacetic acid；
The bacteria cellulose that dry weight is 0.6g is taken after deionized water is cleaned, to be dipped into the wine that 80mL concentration is 4% as matrix again
In stone acid potassium sodium tetrahydrate solution after 15min, 80mL silver plating solutions are taken to be added in sodium potassium tartrate tetrahydrate tetrahydrate solution, then soak
Stain 2h, reduction silver ion is nano-Ag particles, and is incorporated on bacteria cellulose matrix, is finally washed with deionized, and is made
Native cellulose base anti-biotic material containing nano silver particles.
Embodiment 4：
Silver plating solution is first prepared, component includes 0.6%（Wt%）Silver nitrate, 1.5%（V/V）Ethylenediamine and 0.7%（V/V）Trifluoroacetic acid；
The bacteria cellulose that dry weight is 0.8g is taken after deionized water is cleaned, to be dipped into the wine that 120mL concentration is 8% as matrix again
In stone acid potassium sodium tetrahydrate solution after 20min, 120mL silver plating solutions are taken to be added in sodium potassium tartrate tetrahydrate tetrahydrate solution, then
3h is impregnated, reduction silver ion is nano-Ag particles, and is incorporated on bacteria cellulose matrix, is finally washed with deionized, system
Native cellulose base anti-biotic material that must be containing nano silver particles.
Embodiment 5：
Silver plating solution is prepared, component includes 0.8%（Wt%）Silver nitrate, 1.7%（V/V）Ethylenediamine and 1%（V/V）Trifluoroacetic acid；Take again
The bacteria cellulose that dry weight is 1g after deionized water is cleaned, is dipped into the tartaric acid that 100mL concentration is 10% as matrix
In potassium sodium tetrahydrate solution after 30min, 150mL silver plating solutions are taken to be added in sodium potassium tartrate tetrahydrate tetrahydrate solution, then impregnate
2.5h, reduction silver ion is nano-Ag particles, and is incorporated on bacteria cellulose matrix, is finally washed with deionized, and is made
Native cellulose base anti-biotic material containing nano silver particles.
Embodiment 6：
Silver plating solution is prepared, component includes 1%（Wt%）Silver nitrate, 1.6%（V/V）Ethylenediamine and 1.2%（V/V）Trifluoroacetic acid；Take again
The bacteria cellulose that dry weight is 1.5g after deionized water is cleaned, is dipped into the winestone that 150mL concentration is 15% as matrix
In sour potassium sodium tetrahydrate solution after 60min, 150mL silver plating solutions are taken to be added in sodium potassium tartrate tetrahydrate tetrahydrate solution, then soak
Stain 5h, reduction silver ion is nano-Ag particles, and is incorporated on bacteria cellulose matrix, is finally washed with deionized, and is made
Native cellulose base anti-biotic material containing nano silver particles.
Embodiment 7：
Silver plating solution is prepared, component includes 1%（Wt%）Silver nitrate, 2%（V/V）Ethylenediamine and 2%（V/V）Trifluoroacetic acid；Dry weight is taken again
For 2g bacteria cellulose as matrix, after deionized water is cleaned, be dipped into the sodium potassium tartrate tetrahydrate that 200mL concentration is 20%
In tetrahydrate solution after 60min, take 200mL silver plating solutions to be added in sodium potassium tartrate tetrahydrate tetrahydrate solution, then impregnate 5h, also
Former silver ion is nano-Ag particles, and is incorporated on bacteria cellulose matrix, is finally washed with deionized, and is made and contains nanometer
The native cellulose base anti-biotic material of silver particles.
Above-described embodiment obtains bacteria cellulose-base anti-biotic material and carries out electron-microscope scanning, thin with unsupported nano-Ag particles
The electron-microscope scanning of fungin is contrasted, it is evident that being loaded on the bacteria cellulose of bacteria cellulose-base anti-biotic material
Nano-Ag particles, and Argent grain is densely distributed, load capacity is high, more uniform, nano-Ag particles load stabilization is distributed, with reference to figure 2-
4。
And bacteria cellulose-base anti-biotic material film is used for antibacterial experiment, is found to the thin of nano-Ag particles sensitivity
Bacterium, bacteria cellulose-base anti-biotic material prepared by the present invention all have good antibacterial action.
With reference to figure 5, wherein B and C are Ag-carrying bacterial cellulose film in the tablet of bacterial plaque is covered with, and A is natural bacteria
Cellophane, it is evident that B, C film overlay area is without bacterial plaque, and edge does not also grow bacterial plaque, and under the covering of A films still
Bacterial plaque is grown, and edge is without bacteriostasis.It is saccharomyces cerevisiae in figure, Escherichia coli, Staphylococcus aureus etc. also can be selected and make
Bacterial strain is detected for antibiotic property, experimental result is similar with saccharomyces cerevisiae.Illustrate the bacteria cellulose prepared using the method for the present invention
Base anti-biotic material fungistatic effect is notable.
Claims (10)
1. a kind of preparation method of bacteria cellulose-base anti-biotic material, it is characterized in that using bacteria cellulose as matrix, selects nitric acid
Silver makes load silver plating as load silver plating solution, and under the action of ethylenediamine and trifluoroacetic acid through sodium potassium tartrate tetrahydrate tetrahydrate solution
Silver ion reduction in liquid is loaded on the matrix of bacteria cellulose into Argent grain, obtains bacteria cellulose-base anti-biotic material.
2. preparation method according to claim 1, it is characterized in that concretely comprising the following steps：
Prepare silver plating solution, including silver nitrate, ethylenediamine, trifluoroacetic acid；
Bacteria cellulose is taken to be impregnated into sodium potassium tartrate tetrahydrate tetrahydrate solution as matrix,
Silver plating solution is added in sodium potassium tartrate tetrahydrate tetrahydrate solution again, impregnates 0.5-5h,
Bacteria cellulose matrix is washed with deionized, obtains the bacteria cellulose-base anti-biotic material containing nano silver particles.
3. preparation method according to claim 2, it is characterized in that the silver plating solution prepared, including weight fraction are 0.1-1%'s
The trifluoroacetic acid that ethylenediamine that silver nitrate, volume fraction are 1-2%, volume fraction are 0.05-2%.
4. the preparation method according to Claims 2 or 3, it is characterized in that taking bacteria cellulose to be impregnated into quality as matrix
5-60min in the sodium potassium tartrate tetrahydrate tetrahydrate solution that concentration is 2%-20%.
5. preparation method according to claim 4, it is characterized in that will be identical with sodium potassium tartrate tetrahydrate tetrahydrate liquor capacity
Silver plating solution be added in sodium potassium tartrate tetrahydrate tetrahydrate solution, impregnate 0.5-5h.
A kind of 6. bacteria cellulose-base anti-biotic material, it is characterized in that being prepared via a method which：Using bacteria cellulose as matrix, choosing
By the use of silver nitrate as load silver plating solution, and under the action of ethylenediamine and trifluoroacetic acid, make through sodium potassium tartrate tetrahydrate tetrahydrate solution
The silver ion reduction in silver plating solution is carried into Argent grain, and is loaded on the matrix of bacteria cellulose, bacteria cellulose-base is obtained and resists
Bacterium material.
7. anti-biotic material according to claim 6, it is characterized in that what is prepared concretely comprises the following steps：
Prepare silver plating solution, including silver nitrate, ethylenediamine, trifluoroacetic acid；
Bacteria cellulose is taken to be impregnated into sodium potassium tartrate tetrahydrate tetrahydrate solution as matrix,
Silver plating solution is added in sodium potassium tartrate tetrahydrate tetrahydrate solution again, impregnates 0.5-5h,
Bacteria cellulose matrix is washed with deionized, obtains the bacteria cellulose-base anti-biotic material containing nano silver particles.
8. anti-biotic material according to claim 7, it is characterized in that in preparation process：The silver plating solution of preparation, including weight point
The trifluoroacetic acid that ethylenediamine that silver nitrate that number is 0.1-1%, volume fraction are 1-2%, volume fraction are 0.05-2%.
9. the anti-biotic material according to claim 7 or 8, it is characterized in that in preparation process：Bacteria cellulose is taken as matrix,
It is impregnated into 5-60min in the sodium potassium tartrate tetrahydrate tetrahydrate solution that mass concentration is 2%-20%.
10. anti-biotic material according to claim 9, it is characterized in that in preparation process：Will be with sodium potassium tartrate tetrahydrate tetrahydrate
The identical silver plating solution of liquor capacity is added in sodium potassium tartrate tetrahydrate tetrahydrate solution, impregnates 0.5-5h.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111841496A (en) * | 2020-07-24 | 2020-10-30 | 泉州南京大学环保产业研究院 | Method for rapidly preparing silver-loaded activated carbon |
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