CN104511045B - A kind of preparation method of the polyvinyl alcohol containing nano silver/chitosan nano fiber membrane dressing - Google Patents
A kind of preparation method of the polyvinyl alcohol containing nano silver/chitosan nano fiber membrane dressing Download PDFInfo
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- CN104511045B CN104511045B CN201310441908.1A CN201310441908A CN104511045B CN 104511045 B CN104511045 B CN 104511045B CN 201310441908 A CN201310441908 A CN 201310441908A CN 104511045 B CN104511045 B CN 104511045B
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- polyvinyl alcohol
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- BQCADISMDOOEFD-UHFFFAOYSA-N Silver Chemical compound [Ag] BQCADISMDOOEFD-UHFFFAOYSA-N 0.000 title claims abstract description 60
- 229920001661 Chitosan Polymers 0.000 title claims abstract description 39
- 239000002121 nanofiber Substances 0.000 title claims abstract description 36
- 239000004372 Polyvinyl alcohol Substances 0.000 title claims abstract description 35
- 239000012528 membrane Substances 0.000 title claims abstract description 35
- 229920002451 polyvinyl alcohol Polymers 0.000 title claims abstract description 35
- 238000002360 preparation method Methods 0.000 title claims abstract description 16
- 238000010041 electrostatic spinning Methods 0.000 claims abstract description 15
- SXRSQZLOMIGNAQ-UHFFFAOYSA-N Glutaraldehyde Chemical compound O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 claims abstract description 10
- 229940068984 polyvinyl alcohol Drugs 0.000 claims description 31
- 235000019422 polyvinyl alcohol Nutrition 0.000 claims description 31
- 238000000034 method Methods 0.000 claims description 16
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 15
- 238000004132 cross linking Methods 0.000 claims description 13
- SQGYOTSLMSWVJD-UHFFFAOYSA-N silver(I) nitrate Inorganic materials [Ag+].[O-]N(=O)=O SQGYOTSLMSWVJD-UHFFFAOYSA-N 0.000 claims description 11
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 11
- 238000003756 stirring Methods 0.000 claims description 9
- 235000019441 ethanol Nutrition 0.000 claims description 8
- 238000009987 spinning Methods 0.000 claims description 8
- 229920000036 polyvinylpyrrolidone Polymers 0.000 claims description 7
- 235000013855 polyvinylpyrrolidone Nutrition 0.000 claims description 7
- 238000013019 agitation Methods 0.000 claims description 6
- 239000001267 polyvinylpyrrolidone Substances 0.000 claims description 6
- 230000008569 process Effects 0.000 claims description 5
- 239000012046 mixed solvent Substances 0.000 claims description 4
- 239000002904 solvent Substances 0.000 claims description 4
- 239000005030 aluminium foil Substances 0.000 claims description 3
- 239000007788 liquid Substances 0.000 claims description 3
- 239000004332 silver Substances 0.000 claims description 3
- 229910052709 silver Inorganic materials 0.000 claims description 3
- 239000003381 stabilizer Substances 0.000 claims description 3
- 240000007594 Oryza sativa Species 0.000 claims description 2
- 235000007164 Oryza sativa Nutrition 0.000 claims description 2
- 239000003638 chemical reducing agent Substances 0.000 claims description 2
- 239000002994 raw material Substances 0.000 claims description 2
- 235000009566 rice Nutrition 0.000 claims description 2
- 150000001412 amines Chemical class 0.000 claims 1
- 150000002085 enols Chemical class 0.000 claims 1
- 230000029663 wound healing Effects 0.000 abstract description 6
- 230000003115 biocidal effect Effects 0.000 abstract description 5
- 239000000463 material Substances 0.000 abstract description 4
- 150000001875 compounds Chemical class 0.000 abstract description 2
- 239000000243 solution Substances 0.000 description 28
- 239000002131 composite material Substances 0.000 description 19
- ZMXDDKWLCZADIW-UHFFFAOYSA-N N,N-Dimethylformamide Chemical compound CN(C)C=O ZMXDDKWLCZADIW-UHFFFAOYSA-N 0.000 description 15
- 206010052428 Wound Diseases 0.000 description 7
- 208000027418 Wounds and injury Diseases 0.000 description 7
- 239000000835 fiber Substances 0.000 description 6
- 239000007853 buffer solution Substances 0.000 description 5
- 108010010803 Gelatin Proteins 0.000 description 4
- 239000008273 gelatin Substances 0.000 description 4
- 229920000159 gelatin Polymers 0.000 description 4
- 235000019322 gelatine Nutrition 0.000 description 4
- 235000011852 gelatine desserts Nutrition 0.000 description 4
- 239000002245 particle Substances 0.000 description 4
- 238000007789 sealing Methods 0.000 description 4
- 229910001961 silver nitrate Inorganic materials 0.000 description 4
- 238000005054 agglomeration Methods 0.000 description 3
- 230000002776 aggregation Effects 0.000 description 3
- 230000000844 anti-bacterial effect Effects 0.000 description 3
- 230000005611 electricity Effects 0.000 description 3
- 238000009736 wetting Methods 0.000 description 3
- IMROMDMJAWUWLK-UHFFFAOYSA-N Ethenol Chemical compound OC=C IMROMDMJAWUWLK-UHFFFAOYSA-N 0.000 description 2
- FOIXSVOLVBLSDH-UHFFFAOYSA-N Silver ion Chemical compound [Ag+] FOIXSVOLVBLSDH-UHFFFAOYSA-N 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 210000000981 epithelium Anatomy 0.000 description 2
- 229910052739 hydrogen Inorganic materials 0.000 description 2
- 239000001257 hydrogen Substances 0.000 description 2
- 125000002887 hydroxy group Chemical group [H]O* 0.000 description 2
- 229920002521 macromolecule Polymers 0.000 description 2
- BDAGIHXWWSANSR-UHFFFAOYSA-N methanoic acid Natural products OC=O BDAGIHXWWSANSR-UHFFFAOYSA-N 0.000 description 2
- 230000009467 reduction Effects 0.000 description 2
- 238000001291 vacuum drying Methods 0.000 description 2
- IXPNQXFRVYWDDI-UHFFFAOYSA-N 1-methyl-2,4-dioxo-1,3-diazinane-5-carboximidamide Chemical compound CN1CC(C(N)=N)C(=O)NC1=O IXPNQXFRVYWDDI-UHFFFAOYSA-N 0.000 description 1
- OSWFIVFLDKOXQC-UHFFFAOYSA-N 4-(3-methoxyphenyl)aniline Chemical compound COC1=CC=CC(C=2C=CC(N)=CC=2)=C1 OSWFIVFLDKOXQC-UHFFFAOYSA-N 0.000 description 1
- 241000894006 Bacteria Species 0.000 description 1
- 208000035143 Bacterial infection Diseases 0.000 description 1
- 102000008186 Collagen Human genes 0.000 description 1
- 108010035532 Collagen Proteins 0.000 description 1
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 description 1
- 239000007836 KH2PO4 Substances 0.000 description 1
- 229920003171 Poly (ethylene oxide) Polymers 0.000 description 1
- 238000004833 X-ray photoelectron spectroscopy Methods 0.000 description 1
- DHKHKXVYLBGOIT-UHFFFAOYSA-N acetaldehyde Diethyl Acetal Natural products CCOC(C)OCC DHKHKXVYLBGOIT-UHFFFAOYSA-N 0.000 description 1
- 125000002777 acetyl group Chemical class [H]C([H])([H])C(*)=O 0.000 description 1
- 208000022362 bacterial infectious disease Diseases 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 210000001124 body fluid Anatomy 0.000 description 1
- 239000010839 body fluid Substances 0.000 description 1
- 238000009835 boiling Methods 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 229920001436 collagen Polymers 0.000 description 1
- 239000003431 cross linking reagent Substances 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- BNIILDVGGAEEIG-UHFFFAOYSA-L disodium hydrogen phosphate Chemical compound [Na+].[Na+].OP([O-])([O-])=O BNIILDVGGAEEIG-UHFFFAOYSA-L 0.000 description 1
- 229910000397 disodium phosphate Inorganic materials 0.000 description 1
- 235000019800 disodium phosphate Nutrition 0.000 description 1
- 238000004090 dissolution Methods 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 235000019253 formic acid Nutrition 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 230000003179 granulation Effects 0.000 description 1
- 238000005469 granulation Methods 0.000 description 1
- 210000001126 granulation tissue Anatomy 0.000 description 1
- 238000005213 imbibition Methods 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 230000009545 invasion Effects 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 1
- 235000019796 monopotassium phosphate Nutrition 0.000 description 1
- 238000005192 partition Methods 0.000 description 1
- 230000035699 permeability Effects 0.000 description 1
- 229920000151 polyglycol Polymers 0.000 description 1
- 239000010695 polyglycol Substances 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 235000010413 sodium alginate Nutrition 0.000 description 1
- 239000000661 sodium alginate Substances 0.000 description 1
- 229940005550 sodium alginate Drugs 0.000 description 1
- 239000004753 textile Substances 0.000 description 1
- 229920002554 vinyl polymer Polymers 0.000 description 1
- 229920003169 water-soluble polymer Polymers 0.000 description 1
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- Artificial Filaments (AREA)
- Materials For Medical Uses (AREA)
Abstract
The present invention relates to a kind of polyvinyl alcohol containing nano silver/chitosan nano fiber membrane dressing and preparation.Nano silver is incorporated into polyvinyl alcohol/chitosan nano fiber membrane by the nanofiber film dressing using polyvinyl alcohol and chitosan compound as base material, by electrostatic spinning, then makes nano fibrous membrane be crosslinked to obtain using glutaraldehyde.The dressing that the present invention obtains has the characteristics that the environment that preparation process is simple, can provide moistening for wound healing and has antibiotic property.
Description
Technical field
The present invention relates to a kind of Biomedical Textiles, specifically a kind of polyvinyl alcohol/chitosan containing nano silver
The preparation method of nanofiber film dressing.
Background technology
Britain professor Winter in 1962 proposes moisture treatment theory, and the dry wound for confirming and being exposed in air
Choma border is compared, and the moist environment for moistening and being formed after the medical dressing application with permeability can promote the formation of epithelium,
Accelerating wound healing.Various moist dressings come into being since then, research shows that ideal medical dressing should have following work(
Energy:1. wound fluid can be absorbed, surface of a wound wetting is kept, the loss of body fluid and moisture is prevented;2. resisting the invasion of bacterium, prevent
Infection;3. promoting the adherency and growth etc. of granulation and epithelial tissue.
In order to keep the wetability of the surface of a wound, many water-soluble high-molecular materials are used as the raw material of medical dressing, including gather
Vinyl alcohol, polyvinylpyrrolidone, polyethylene oxide etc. contain a large amount of hydroxyl in these molecules, can be formed with hydrone greatly
Hydrogen bond is measured, therefore, prepared medical dressing can absorb moisture, and the environment of wetting is provided for wound healing.Merely with water-soluble
Property macromolecule medical dressing is made, in use can because of it is high molecular dissolving and scatter and disappear its stability therefore often want
It is blended with polysaccharide family macromolecule (such as chitosan, sodium alginate, collagen), it could be kept by being used in combination crosslinking agent to carry out crosslinking
The stability of pattern.
The medical dressing made of hydrophilic high molecular material can provide wet environment for wound healing, while can also lead
It causes the chance of wound circumference bacterial infection to increase, therefore, also to enhance the antibiotic property of medical dressing.Silver has broad spectrum antibacterial,
Nano silver is added in dressings can assign the antibiotic property of dressing.Nano-Ag particles activity is made in the form of nanotechnology to become larger, holds
Agglomeration easily occurs, it is difficult to be directly added into dressing.
Nano silver can be made once-forming with nanofiber using method of electrostatic spinning, nano silver is made to be evenly distributed in dressing
In, effectively reduce nano silver fall off and agglomeration.Meanwhile method of electrostatic spinning prepares that nanofiber is simple for process, and structure can
Control, prepared nano fibrous membrane have the characteristics that large specific surface area, porosity are big, are conducive to the adherency and life of wound cells
It is long.
Application number 200710171324.1, it is entitled " fine for medical dressing composite Nano containing nano silver gelatin/chitosan
Tie up felt and preparation " Chinese invention patent application, disclose and a kind of be made of the gelatin/chitosan nanofiber containing nano silver
Composite nanometer fiber felt.Preparation method is:Using formic acid as solvent, gelatin solution is mixed according to a certain percentage with chitosan solution
It closes, silver nitrate is added, nanofiber mats are made with method of electrostatic spinning;Make the silver ion reduction Cheng Yin in fiber with ultraviolet light
Particle.It using nano-Ag particles diameter very little made from this method, and is evenly distributed in fine felt, utilizes the strong antibacterial of nano silver
Effect can be made into medical dressing.Because the water absorbing capacity of gelatin itself is relatively low, this composite nanometer fiber felt absorbs exudation
Liquid energy power is not high.
Application No. is 201010248219.5, it is entitled " a kind of chitosan/polyvinyl alcohol sponge dressing containing nano silver
The Chinese invention patent application of preparation method " discloses a kind of preparation of the chitosan/polyvinyl alcohol sponge dressing containing nano silver
Method:In Polyvinyl acetal reaction, nano silver/polyglycol solution is added and polyvinyl alcohol/chitosan alcohol is blended
The chitosan/polyvinyl alcohol sponge dressing containing nano silver is made in liquid.There is good biofacies using the dressing that this method obtains
Capacitive, water imbibition and antibiotic property, but this sponge dressing specific surface area is small, porosity is small, adherency and growth to wound cells
Facilitation is limited.
Invention content
The technical problem to be solved by the present invention is to overcome defect of the existing technology, provide a kind of poly- containing nano silver
Vinyl alcohol/chitosan nano fiber membrane dressing and preparation, the nano fibrous membrane using polyvinyl alcohol and chitosan compound as base material,
Nano silver is incorporated into polyvinyl alcohol/chitosan composite nano fibre film by electrostatic spinning, then makes to receive using glutaraldehyde
Rice tunica fibrosa is crosslinked to obtain.
Polyvinyl alcohol containing the nano silver/chitosan nano fiber membrane dressing, specific preparation method are as follows:
(1) poly-vinyl alcohol solution is prepared
Polyvinyl alcohol is dissolved in the mixed solvent (water of water and ethyl alcohol:Ethyl alcohol mass ratio is 8:15) in, magnetic agitation is to complete
Fully dissolved obtains a concentration of 8~20% poly-vinyl alcohol solution.
(2) chitosan solution is prepared
Chitosan is dissolved in acetum (mass concentration 1%), magnetic agitation to being completely dissolved, obtain a concentration of 3~
10% chitosan solution.
(3) nano silver colloidal sol is prepared
According to following reactions nano silver colloidal sol is prepared by presoma of silver nitrate:
Polyvinylpyrrolidone (stabilizer as nano silver) is dissolved in n,N-Dimethylformamide (as solvent and also
Former agent) in, it is made into reducing solution.By AgNO3Solution is added dropwise in reducing solution while stirring, is added dropwise, and continues to stir 6h,
Nano silver colloidal sol is made.
(4) polyvinyl alcohol/chitosan/nano silver spinning solution is prepared
Take poly-vinyl alcohol solution and chitosan solution, in mass ratio 100:4 are mixed, and are added dropwise after magnetic agitation is uniform
Enter nano silver colloidal sol, the mass ratio of poly-vinyl alcohol solution and nano silver colloidal sol is 100:6, it is added dropwise, continues to stir 2h, be made into
Spinning solution.
(5) electrostatic spinning
Polyvinyl alcohol/the chitosan prepared in step (4)/nano silver spinning solution is subjected to electrostatic spinning and obtains nanofiber
Film is received using aluminium foil, and electrostatic spinning process parameter is:Voltage is 10kV, and syringe pump fltting speed is 20uL/min, receive away from
From for 12cm, temperature is room temperature.
(6) glutaraldehyde cross-linking
The nano fibrous membrane prepared in step (5) is placed in 1~3h of sealing crosslinking in glutaraldehyde steam.
It is an advantage of the invention that:The present invention uses polyvinyl alcohol containing nano silver/chitosan nano prepared by method of electrostatic spinning
Fiber film dressing has the characteristics that the environment that preparation process is simple, can provide moistening for wound healing and has antibiotic property.
The present invention is using n,N-Dimethylformamide as solvent and reducing agent, and polyvinylpyrrolidone is as nano silver
Stabilizer, presoma-silver nitrate of nano silver is added in polyvinylpyrrolidone/n,N-Dimethylformamide solution,
Make silver ion reduction at nano silver colloidal sol, the nano silver diameter very little obtained in this way avoids and is directly added into nano silver powder.It adopts
Nano silver can be made once-forming with nanofiber with method of electrostatic spinning, allow nano-Ag particles to be attached to polyvinyl alcohol/chitosan multiple
Close in nanofiber surface or embedded fiber, can effectively reduce in this way nano silver fall off and agglomeration, make the nanometer to be formed
Silver is more uniformly distributed in nanofiber.Using the broad spectrum antibacterial of nano silver, composite nano-fiber membrane prepared by the present invention
It can be used as medical dressing.Polyvinyl alcohol is a kind of water soluble polymer, and containing a large amount of hydroxyl, a large amount of hydrogen can be formed with hydrone
Key, therefore, composite nano-fiber membrane prepared by the present invention can absorb moisture, and the environment of wetting is provided for wound healing, and sharp
After being crosslinked with glutaraldehyde, stability of the nano fibrous membrane under wet environment is also improved.
Description of the drawings
It, below will be to specific in order to illustrate more clearly of the specific embodiment of the invention or technical solution in the prior art
Embodiment or attached drawing needed to be used in the description of the prior art are briefly described, it should be apparent that, in being described below
Attached drawing is some embodiments of the present invention, for those of ordinary skill in the art, before not making the creative labor
It puts, other drawings may also be obtained based on these drawings.
Fig. 1 is the scanning electron microscope (SEM) photograph of the composite nano-fiber membrane containing nano silver made from embodiment 1;
Fig. 2 is the scanning electron microscope (SEM) photograph of the composite nano-fiber membrane containing nano silver made from embodiment 2;
Fig. 3 is the scanning electron microscope (SEM) photograph of the composite nano-fiber membrane containing nano silver made from embodiment 3;
Fig. 4 is that composite nano-fiber membrane containing nano silver made from embodiment 1 impregnates the scanning electricity after 1h in PBS buffer solution
Mirror figure;
Fig. 5 is that composite nano-fiber membrane containing nano silver made from embodiment 2 impregnates the scanning electricity after 1h in PBS buffer solution
Mirror figure;
Fig. 6 is that composite nano-fiber membrane containing nano silver made from embodiment 3 impregnates the scanning electricity after 1h in PBS buffer solution
Mirror figure;
Fig. 7 is the x-ray photoelectron spectroscopy figure of the composite nano-fiber membrane containing nano silver made from embodiment 3;It can be with by Fig. 1~3
Find out, with the extension of crosslinking time, the adhesion degree between nanofiber increases, and the hole of composite nano-fiber membrane is reduced.
As the extension of crosslinking time, the holding fiber morphology of composite nano-fiber membrane are stablized it can be seen from Fig. 4~6
Property enhancing.
As seen from Figure 7, there are nano silvers on the surface of composite nano-fiber membrane.
Specific implementation mode
With reference to example, the present invention is described further, but the scope of protection of present invention is not limited to example
The range of expression.
A kind of polyvinyl alcohol containing nano silver/chitosan nano fiber membrane dressing and preparation, specific implementation mode are as follows:
Embodiment 1
Weigh the mixed solvent (water that 8g polyvinyl alcohol is dissolved in 92g water and ethyl alcohol:Ethyl alcohol mass ratio is 8:15) it in, is made into
The poly-vinyl alcohol solution that 100g mass fractions are 8%.The acetum that 0.24g chitosans are dissolved in 7.76g1% is weighed, 8g is made into
The chitosan solution that mass fraction is 3%.In boiling water bath, while stirring by the silver nitrate solution of a concentration of 400g/L of 0.6ml
Polyvinylpyrrolidone/n,N-Dimethylformamide solution is added dropwise, and (0.6g polyvinylpyrrolidones are dissolved in 5.4mL N, N-
Dimethylformamide) in, continue insulated and stirred 6h and nano silver colloidal sol is made.After above-mentioned polyethanol and chitosan solution are mixed,
Above-mentioned nano silver colloidal sol is added dropwise while stirring, is added dropwise, continues stirring 2h and is made into spinning solution.Utilize electrostatic spinning apparatus
It carries out spinning and obtains nano fibrous membrane, voltage 10kV, syringe pump fltting speed is 20uL/min, is received using aluminium foil, is received
Distance is 12cm.The glutaraldehyde for measuring 50mL50% is added in the drier of 180mm, after being preheated one hour at 60 DEG C, by nanometer
Tunica fibrosa is placed in inside drier sealing crosslinking 1h on a porous partition board, is then placed in the nano fibrous membrane after crosslinking
Dry 72h, obtains the sample of composite nano-fiber membrane containing nano silver A1 in vacuum drying chamber.
Embodiment 2
The concentration of poly-vinyl alcohol solution is expanded as 20%, the sealing crosslinking time of nano fibrous membrane extends to 2h, other
Reagent and dosage and process conditions are constant, repeat embodiment 1, obtain the sample of composite nano-fiber membrane containing nano silver A2.
Embodiment 3
The concentration of chitosan solution is expanded as 10%, the sealing crosslinking time of nano fibrous membrane extends to 3h, other examinations
Agent and dosage and process conditions are constant, repeat embodiment 1, obtain the sample of composite nano-fiber membrane containing nano silver A3.
Above-mentioned sample tests its steady dissolution using following methods:
Sample after crosslinking is separately immersed in the PBS buffer solutions of PH=7.4 (by the KH2PO4 of a concentration of 0.1mol/L
The Na2HPO4 solution of solution and 0.1mol/L are 1 by volume:4 are obtained by mixing) in handle 1h, after taking-up wash with distilled water,
Vacuum drying oven is placed in dry for 24 hours under conditions of 40 DEG C.Then, using scanning electron microscope sem, (NoVaNaVo SEM430, FEI are public
Department) microscopic appearance of the observation sample before and after impregnating PBS buffer solutions.
Claims (3)
1. a kind of preparation method of the polyvinyl alcohol containing nano silver/chitosan nano fiber membrane dressing, which is characterized in that with poly- second
Enol, chitosan, nano silver are raw material, prepare nano fibrous membrane by method of electrostatic spinning, then make nanofiber with glutaraldehyde
Film is crosslinked and is made;Include the following steps:
(1) poly-vinyl alcohol solution is prepared
Polyvinyl alcohol is dissolved in the in the mixed solvent of water and ethyl alcohol, magnetic agitation obtains a concentration of 8~20% to being completely dissolved
The mass ratio of the in the mixed solvent of poly-vinyl alcohol solution, wherein water and ethyl alcohol, water and ethyl alcohol is 8:15;
(2) chitosan solution is prepared
Chitosan is dissolved in the acetum of mass concentration 1%, magnetic agitation obtains a concentration of 3~10% to being completely dissolved
Chitosan solution;
(3) nano silver colloidal sol is prepared
It will be dissolved in the N as solvent and reducing agent, N- dimethyl formyls as the polyvinylpyrrolidone of the stabilizer of nano silver
In amine, it is made into reducing solution;By AgNO3Solution is added dropwise in reducing solution while stirring, is added dropwise, and continues to stir 6h, system
At nano silver colloidal sol;
(4) polyvinyl alcohol/chitosan/nano silver spinning solution is prepared
Take poly-vinyl alcohol solution and chitosan solution, in mass ratio 100:4 are mixed, and are added dropwise and are received after magnetic agitation is uniform
The mass ratio of rice silver sol, poly-vinyl alcohol solution and nano silver colloidal sol is 100:6, it is added dropwise, continues to stir 2h, be made into spinning
Liquid;
(5) electrostatic spinning
Polyvinyl alcohol/the chitosan prepared in step (4)/nano silver spinning solution is subjected to electrostatic spinning and obtains nano fibrous membrane,
It is received using aluminium foil;
(6) glutaraldehyde cross-linking
The nano fibrous membrane prepared in step (5) is placed in glutaraldehyde steam and is sealed crosslinking.
2. preparation method according to claim 1, it is characterised in that:The electrostatic spinning process parameter, voltage 10kV,
Syringe pump fltting speed is 20uL/min, and it is 12cm to receive distance, and temperature is room temperature.
3. preparation method according to claim 1, it is characterised in that:The glutaraldehyde cross-linking time is 1~3h.
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