CN107898661A - A kind of purposes of mussel polysaccharide - Google Patents
A kind of purposes of mussel polysaccharide Download PDFInfo
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- CN107898661A CN107898661A CN201711224026.4A CN201711224026A CN107898661A CN 107898661 A CN107898661 A CN 107898661A CN 201711224026 A CN201711224026 A CN 201711224026A CN 107898661 A CN107898661 A CN 107898661A
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- mussel
- mussel polysaccharide
- polysaccharide
- degreasing
- skin care
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/73—Polysaccharides
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
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- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/805—Corresponding aspects not provided for by any of codes A61K2800/81 - A61K2800/95
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/80—Process related aspects concerning the preparation of the cosmetic composition or the storage or application thereof
- A61K2800/81—Preparation or application process involves irradiation
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- Chemical Kinetics & Catalysis (AREA)
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- Polymers & Plastics (AREA)
- Organic Chemistry (AREA)
- Gerontology & Geriatric Medicine (AREA)
- Dermatology (AREA)
- Cosmetics (AREA)
- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
Abstract
The invention discloses a kind of purposes of mussel polysaccharide, mussel polysaccharide can be used for preparing anti-aging skin care product, which is surfactant or skin care milk or face cream or facial mask.Above-mentioned mussel polysaccharide carries out extraction acquisition through degreasing, complex enzyme Microwave Extraction, chromatography, alcohol precipitation.Have the beneficial effect that：Mussel polysaccharide of the present invention is used to prepare mussel polysaccharide purity height, energy H excessive directly in scavenger-cell2O2, strengthen cell viability, promote the growing multiplication of cell, keep the anti-aging skin care product of the reproducibility of cell；The skin care item do not stimulate also with Nantural non-toxic, gently, the advantage with moisture-keeping efficacy.
Description
Technical field
The present invention relates to natural products deep process technology field, more particularly to a kind of purposes of mussel polysaccharide.
Background technology
With expanding economy, land resources makes full use of, and the fast development of marine aquaculture, in recent years ocean
The exploitation of resource becomes increasingly urgent.There are abundant exploitation and marine pharmaceutical resource leaved for development in ocean, so exploitation sea
Foreign drug resource becomes to be even more important.Different marine pharmaceutical resources has different efficacy of drugs, and polysaccharide can not only improve
Body's immunity, also with antitumor, radioresistance and anticoagulation and the multiple biological activities such as anti-oxidant.A variety of biologies of polysaccharide
Prevention of the activity for the century problem such as tumour is of great significance.And marine resources have the characteristics that it is regional, seasonal,
Therefore, the utilization of the optimal extraction technology of polysaccharide and polysaccharide formula food have important application value.Polysaccharide is a kind of
Large biological molecule.It is present in plant, animal and microbial cell, is the important component of living organism.Pass through dehydration
Glycosidic bond is formed between each monose, glycosidic bond is connected in a manner of linear or branch, forms chain polymer.Numerous studies table
Bright, polysaccharide has anti-aging, antitumor, immunological regulation, anti-hepatitis virus infection, AIDS virus resisting isoreactivity, prior
It is that polysaccharide toxic side effect is small, therefore, polysaccharide will solve the century problem such as cancer and AIDS in prevention etc..Mussel polysaccharide is more
For α configurations activity, fewer is beta comfiguration activity.Mussel, belongs to Mollusca mussel, belongs to Mollusca, lamellibranchiata, different column
Mesh, Mytilidae.Marine rainbow is commonly called as, is also known as mussel.Mussel polysaccharide glycosidic bond is mainly 1-4 glycosidic bonds, 1-6 glycosidic bonds, 1-3 glucosides
Key, 1-2 glycosidic bonds, wherein based on 1-4 glycosidic bonds.And play mainly 1-4 glycosidic bonds and the 1-6 glucosides of antitumor action
Key.Mussel polysaccharide has anti-oxidant, immunological regulation, antitumor isoreactivity, and toxic side effect is small, therefore, mussel polysaccharide in recent years
Given birth to by extensive concern, and by a large amount of mussel formula foods and pharmaceutical applications.
The content of the invention
It is an object of the invention to provide a kind of purposes of mussel polysaccharide, which is to prepare mussel polysaccharide purity height, energy
Excessive H in direct scavenger-cell2O2, enhancing cell viability, the growing multiplication for promoting cell, keep cell reproducibility it is anti-
Aging skin care item.
The present invention is directed to the problem of being mentioned in above-mentioned technology, and the technical solution taken is：A kind of purposes of mussel polysaccharide, makes a gift of
Pattra sugar can be used for preparing anti-aging skin care product, which is surfactant or skin care milk or face cream or facial mask.Above-mentioned skin care
Product can promote the high expression that peroxiredoxin is stablized, so as to pass through H excessive in direct scavenger-cell2O2, people can be weakened
Aging correlation heterochromatin aggregation caused by diploid cell, enhancing cell viability, the growing multiplication for promoting cell, reduces cell
Half beta lactose glycosides enzyme content, the aging for intervening cell, and then delaying cell aging；The skin care at the same time can also strengthen on mitochondria
The activity of succinate dehydrogenase so that mitochondria can continue to as cellular activity supplying energy, by keeping the reproducibility of cell,
To improve the activation of the transcription factor of redox signal sensitivity, so as to maintain " youth " state of cell；And the skin care item also have
The advantage for have Nantural non-toxic, not stimulating, there is moisture-keeping efficacy gently.
Preferably, mussel polysaccharide carries out extraction acquisition through degreasing, complex enzyme-Microwave Extraction, chromatography, alcohol precipitation.
Preferably, degreasing is：Mussel fresh meat is rubbed, homogenate, with petroleum ether degreasing, be made through low temperature drying, crushing
Degreasing Mussel Powder, the histocyte of polysaccharide have lipid encirclement more, often contain more albumen in tissue, so first to be taken off
Fat processing, the step can remove the fat in mussel, and the presence of fat can produce bad shadow to the flavor of product, color and luster etc.
Ring, while be easy to cause the muddiness of hydrolyzate, influence the activity of mussel polysaccharide, contain petroleum ether weight 0.023- in petroleum ether
0.036% anphotericin and the 5-flurocytosine of petroleum ether weight 0.014-0.016%, anphotericin and 5-flurocytosine add
Petroleum ether degreasing speed can not only be greatly improved by entering, and reduce the dosage of petroleum ether, and degreasing is thorough, can be with mussel cell membrane
Material is combined, and damages the permeability of film, is prepared for the polysaccharide component in extraction cell membrane so that the purity of polysaccharide is high.
Preferably, complex enzyme-Microwave Extraction is：It is degreasing Mussel Powder is soluble in water so that concentration of substrate 3-5%, is pressed
The enzyme buffer liquid that enzyme concentration adds for 0.8-1.3%, the Microwave Extraction in the case where temperature is 50-60 DEG C, microwave power is 400-600W
40-60min, 95-100 DEG C of enzyme deactivation 18-25min, are cooled to room temperature, and centrifuge 10-20min in 3000-5000r/min, take supernatant
Liquid, then with Sevage method deproteinizeds, by the 1/4-1/2 of supernatant concentration to original volume, loads bag filter dialysis, removes small point
Sub- impurity and salt, up to mussel polysaccharide extracting solution, microwave method extractor is carried out it is intermittent vacuumize and vacuum breaker, utilize
Vacuum impulse strengthens mass transfer, and the mechanism that vacuum impulse strengthens mass transfer is：When vacuumizing moisture in mussel cell tissue can because
Depressurize and gasify, cause pressure in mussel cell tissue to be more than extracellularly, having dissolved the water of polysaccharide can force under differential pressure action
Through mussel cell tissue, when vacuum breaker, mussel histocyte external pressure is more than internal pressure, the moisture outside mussel histocyte because
Differential pressure action and quickly entering removes dissolving polysaccharide inside mussel histocyte, the alternating of this mussel histocyte external and internal pressure becomes
Change enhances moisture and the polysaccharide mass transfer being dissolved in moisture, improves the extraction efficiency of polysaccharide, residual rate reduction, and protease
Add the albumen that can be connected on fast shut-off polysaccharide sugar chain, most of albumen can be removed, in conjunction with Sevage method removing proteins,
The removal rate of protein is further increased, while decreases the loss of polysaccharide, the original structure of polysaccharide and activity can be preserved.
Above-mentioned enzyme buffer liquid for be 6.8-7.5 containing papain and neutral proteinase, pH phosphate buffer, papain and
The weight ratio of neutral proteinase is 1:0.76-0.83, enzyme is dissolved in buffer solution, and the pH of whole enzymatic reaction system can be made to tie up all the time
Hold in claimed range, it is not necessary to adjust pH repeatedly, reduce labor intensity or energy consumption.
Preferably, chromatography is：Mussel polysaccharide extracting solution is loaded to ME-1 macroporous adsorption resin chromatography columns, with 1.5-
De-, collection eluent is washed with deionized water in the speed of 2.5ml/min, and mussel polysaccharide, which has, is adsorbed in hydrophobic resin surface apertures
Ability, and then mussel polysaccharide is extracted using hydrophobic resin, and ME-1 large pore resin absorption columns are high to mussel polysaccharide adsorption rate, and
Easily elution, regeneration are also easier.
Preferably, alcohol precipitation is：Eluent is concentrated under reduced pressure into 4-6mg/ml, 3-5 times of 95% ethanol of volume is added, in 2-5
10-15h is stood under the conditions of DEG C, collected after centrifugation precipitation, is redissolved with water, be freeze-dried to obtain mussel polysaccharide sterling.
Compared with prior art, the advantage of the invention is that：1）Mussel polysaccharide of the present invention is used to prepare mussel polysaccharide purity
Height, energy H excessive directly in scavenger-cell2O2, strengthen cell viability, promote the growing multiplication of cell, keep the reproducibility of cell
Anti-aging skin care product；2）The skin care item do not stimulate also with Nantural non-toxic, gently, the advantage with moisture-keeping efficacy；3）This hair
The preparation method simple possible of bright skin care item, mechanized operation is high into degree, takes short, it is possible to increase the utilization rate of raw material, makes a gift of
The recovery rate of pattra sugar is high, purity is high, is easy to industrial-scale production, has good application value and market potential；4）This
Invention is combined using ultramicro grinding and grinding aid so that part mussel breaking-wall cell, and then glycocalix is largely discharged
Come, so that polysaccharide dissolution rate increases, while can prevent the polysaccharide structures of macromolecular from occurring to be broken and produce in ultramicro grinding
Raw oligosaccharides, keeps the activity of polysaccharide not to be destroyed.
Embodiment
The present invention program is described further below by embodiment：
Embodiment 1：
A kind of purposes of mussel polysaccharide, mussel polysaccharide can be used for preparing anti-aging skin care product, which is surfactant or skin care
Breast or face cream or facial mask.
Above-mentioned mussel polysaccharide carries out extraction acquisition through degreasing, complex enzyme-Microwave Extraction, chromatography, alcohol precipitation.
Above-mentioned degreasing is：Mussel fresh meat is rubbed, homogenate, with petroleum ether degreasing, degreasing is made and makes a gift of through low temperature drying, crushing
Bei Fen, the 5- of the anphotericin containing petroleum ether weight 0.023-0.036% and petroleum ether weight 0.014-0.016% in petroleum ether
Flucytosine.
Above-mentioned complex enzyme-Microwave Extraction is：Degreasing Mussel Powder is soluble in water so that concentration of substrate 3-5%, by enzyme
Measure the enzyme buffer liquid added for 0.8-1.3%, the Microwave Extraction 40- in the case where temperature is 50-60 DEG C, microwave power is 400-600W
60min, 95-100 DEG C of enzyme deactivation 18-25min, are cooled to room temperature, and centrifuge 10-20min in 3000-5000r/min, take supernatant,
Then Sevage method deproteinizeds are used, by the 1/4-1/2 of supernatant concentration to original volume, loads bag filter dialysis, removes small molecule
Impurity and salt, up to mussel polysaccharide extracting solution.Above-mentioned enzyme buffer liquid is containing papain and neutral proteinase, pH 6.8-
The weight ratio of 7.5 phosphate buffer, papain and neutral proteinase is 1:0.76-0.83.
Above-mentioned chromatography is：Mussel polysaccharide extracting solution is loaded to ME-1 macroporous adsorption resin chromatography columns, with 1.5-2.5ml/
De-, collection eluent is washed with deionized water in the speed of min.
Above-mentioned alcohol precipitation is：Eluent is concentrated under reduced pressure into 4-6mg/ml, 3-5 times of 95% ethanol of volume is added, in 2-5 DEG C of bar
10-15h is stood under part, centrifuges, sediment is redissolved with water, is freeze-dried to obtain mussel polysaccharide sterling.
Embodiment 2：
A kind of purposes of mussel polysaccharide, mussel polysaccharide can be used for preparing anti-aging skin care product, which is surfactant or skin care
Breast or face cream or facial mask.
Above-mentioned mussel polysaccharide carries out extraction acquisition through degreasing, complex enzyme-Microwave Extraction, chromatography, alcohol precipitation.
Above-mentioned degreasing is：Mussel fresh meat is rubbed, homogenate, with petroleum ether degreasing, degreasing is made and makes a gift of through low temperature drying, crushing
Bei Fen, the anphotericin containing petroleum ether weight 0.035% and the 5-flurocytosine of petroleum ether weight 0.014% in petroleum ether.
Above-mentioned complex enzyme-Microwave Extraction is：It is degreasing Mussel Powder is soluble in water so that concentration of substrate 5%, adds thick de-
The beta-amino ethyl sulfonic acid and 0.046 ‰ neopelex of fat Mussel Powder weight 0.035 ‰, are added by enzyme concentration for 0.8%
The enzyme buffer liquid entered, the Microwave Extraction 60min in the case where temperature is 60 DEG C, microwave power is 450W, 100 DEG C of enzyme deactivation 18min, are cooled to
Room temperature, centrifuges 12min in 5000r/min, takes supernatant, then with Sevage method deproteinizeds, by supernatant concentration to original volume
1/4, load bag filter dialysis, small molecular weight impurity and salt removed, up to mussel polysaccharide extracting solution, beta-amino ethyl sulfonic acid and ten
The addition of dialkyl benzene sulfonic acids sodium can be loosened polysaccharide structures, intramolecular enzyme effect site be exposed, beneficial to albumen
The protein portion of glycoprotein is fallen in enzyme hydrolysis, is left sugar chain, improves the speed of protease hydrolyzed glycoprotein and thoroughly spends, reaches meaning
Unimaginable effect, and it can be dissolved in water exclusion, noresidue, has no adverse effects the activity of polysaccharide.Above-mentioned enzyme buffer liquid be containing
The weight ratio of papain and neutral proteinase, the phosphate buffer that pH is 7.5, papain and neutral proteinase is
1:0.76。
Above-mentioned chromatography is：Mussel polysaccharide extracting solution is loaded to ME-1 macroporous adsorption resin chromatography columns, with 1.7ml/min's
De-, collection eluent is washed with deionized water in speed.
Above-mentioned alcohol precipitation is：Eluent is concentrated under reduced pressure into 6mg/ml, adds 3 times of 95% ethanol of volume, it is quiet under the conditions of 5 DEG C
10h is put, centrifuges, sediment is redissolved with water, is freeze-dried to obtain mussel polysaccharide sterling.
Embodiment 3：
A kind of purposes of mussel polysaccharide, mussel polysaccharide can be used for preparing anti-aging skin care product, which is surfactant or skin care
Breast or face cream or facial mask.
Above-mentioned mussel polysaccharide carries out extraction acquisition through degreasing, complex enzyme-Microwave Extraction, chromatography, alcohol precipitation.
Above-mentioned degreasing is：Mussel fresh meat is rubbed, homogenate, with petroleum ether degreasing, degreasing is made and makes a gift of through low temperature drying, crushing
Bei Fen, the anphotericin containing petroleum ether weight 0.03% and the 5-flurocytosine of petroleum ether weight 0.015% in petroleum ether.
Above-mentioned complex enzyme-Microwave Extraction is：Degreasing Mussel Powder is soluble in water so that concentration of substrate 4%, by enzyme concentration
For 1.0% enzyme buffer liquid added, Microwave Extraction 50min, 98 DEG C of enzyme deactivations in the case where temperature is 55 DEG C, microwave power is 500W
20min, is cooled to room temperature, and centrifuges 15min in 4000r/min, takes supernatant, then with Sevage method deproteinizeds, by supernatant
The 1/3 of original volume is concentrated into, loads bag filter dialysis, small molecular weight impurity and salt is removed, up to mussel polysaccharide extracting solution.It is above-mentioned
The phosphate buffer that it is 7.0 containing papain and neutral proteinase, pH that enzyme buffer liquid, which is, papain and neutral protein
The weight ratio of enzyme is 1:0.8.
Above-mentioned chromatography is：Mussel polysaccharide extracting solution is loaded to ME-1 macroporous adsorption resin chromatography columns, with 2.0ml/min's
De-, collection eluent is washed with deionized water in speed.
Above-mentioned alcohol precipitation is：Eluent is concentrated under reduced pressure into 5mg/ml, adds 4 times of 95% ethanol of volume, it is quiet under the conditions of 4 DEG C
12h is put, centrifuges, sediment is redissolved with water, is freeze-dried to obtain mussel polysaccharide sterling.
Routine operation in the operating procedure of the present invention is well known to those skilled in the art, herein without repeating.
Technical scheme is described in detail in embodiment described above, it should be understood that the above is only
For the specific embodiment of the present invention, it is not intended to limit the invention, all any modifications made in the spirit of the present invention,
Supplement or similar fashion replacement etc., should all be included in the protection scope of the present invention.
Claims (9)
- A kind of 1. purposes of mussel polysaccharide, it is characterised in that：The mussel polysaccharide can be used for preparing anti-aging skin care product.
- A kind of 2. purposes of mussel polysaccharide according to claim 1, it is characterised in that：The skin care item are surfactant or shield Skin breast or face cream or facial mask.
- A kind of 3. purposes of mussel polysaccharide according to claim 1, it is characterised in that：The mussel polysaccharide is through degreasing, multiple Synthase-Microwave Extraction, chromatography, alcohol precipitation carry out extraction acquisition.
- A kind of 4. preparation method of mussel polysaccharide according to claim 3, it is characterised in that：The degreasing is：By mussel Fresh meat rubs, homogenate, and with petroleum ether degreasing, degreasing Mussel Powder is made through low temperature drying, crushing.
- A kind of 5. preparation method of mussel polysaccharide according to claim 4, it is characterised in that：Contain stone in the petroleum ether The anphotericin of oily ether weight 0.023-0.036% and the 5-flurocytosine of petroleum ether weight 0.014-0.016%.
- A kind of 6. preparation method of mussel polysaccharide according to claim 3, it is characterised in that：Complex enzyme-the microwave carries It is taken as：It is degreasing Mussel Powder is soluble in water so that concentration of substrate 3-5%, is the enzyme buffer that 0.8-1.3% is added by enzyme concentration Liquid, Microwave Extraction 40-60min, 95-100 DEG C of enzyme deactivation 18-25min in the case where temperature is 50-60 DEG C, microwave power is 400-600W, It is cooled to room temperature, centrifuges 10-20min in 3000-5000r/min, take supernatant, then with Sevage method deproteinizeds, by supernatant Liquid is concentrated into the 1/4-1/2 of original volume, loads bag filter dialysis, removes small molecular weight impurity and salt, is extracted up to mussel polysaccharide Liquid.
- A kind of 7. preparation method of mussel polysaccharide according to claim 6, it is characterised in that：The enzyme buffer liquid is containing wood Melon protease and neutral proteinase, the phosphate buffer that pH is 6.8-7.5.
- A kind of 8. preparation method of mussel polysaccharide according to claim 3, it is characterised in that：The chromatography is：By mussel Polysaccharide extraction liquid is loaded to ME-1 macroporous adsorption resin chromatography columns, and de-, receipts are washed with deionized water with the speed of 1.5-2.5ml/min Collect eluent.
- A kind of 9. preparation method of mussel polysaccharide according to claim 3, it is characterised in that：The alcohol precipitation is：Will elution Liquid is concentrated under reduced pressure into 4-6mg/ml, adds 3-5 times of 95% ethanol of volume, 10-15h, collected after centrifugation are stood under the conditions of 2-5 DEG C Precipitation, is redissolved with water, is freeze-dried to obtain mussel polysaccharide sterling.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201711224026.4A CN107898661A (en) | 2017-11-29 | 2017-11-29 | A kind of purposes of mussel polysaccharide |
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| Application Number | Priority Date | Filing Date | Title |
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| CN201711224026.4A CN107898661A (en) | 2017-11-29 | 2017-11-29 | A kind of purposes of mussel polysaccharide |
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| CN107898661A true CN107898661A (en) | 2018-04-13 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108524315A (en) * | 2018-06-29 | 2018-09-14 | 广州市润研基因科技有限公司 | A kind of novel anti-pollution moisturizing skin makeup liquid and preparation method thereof |
-
2017
- 2017-11-29 CN CN201711224026.4A patent/CN107898661A/en not_active Withdrawn
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108524315A (en) * | 2018-06-29 | 2018-09-14 | 广州市润研基因科技有限公司 | A kind of novel anti-pollution moisturizing skin makeup liquid and preparation method thereof |
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Application publication date: 20180413 |