CN107897766A - One kind is relieved the effect of alcohol compound preparation and preparation method thereof - Google Patents
One kind is relieved the effect of alcohol compound preparation and preparation method thereof Download PDFInfo
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- CN107897766A CN107897766A CN201711171666.3A CN201711171666A CN107897766A CN 107897766 A CN107897766 A CN 107897766A CN 201711171666 A CN201711171666 A CN 201711171666A CN 107897766 A CN107897766 A CN 107897766A
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Classifications
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- A—HUMAN NECESSITIES
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- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
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- A23L17/00—Food-from-the-sea products; Fish products; Fish meal; Fish-egg substitutes; Preparation or treatment thereof
- A23L17/65—Addition of, or treatment with, microorganisms or enzymes
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L29/00—Foods or foodstuffs containing additives; Preparation or treatment thereof
- A23L29/06—Enzymes
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L31/00—Edible extracts or preparations of fungi; Preparation or treatment thereof
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
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- A23L31/10—Yeasts or derivatives thereof
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS, OR NON-ALCOHOLIC BEVERAGES, NOT COVERED BY SUBCLASSES A21D OR A23B-A23J; THEIR PREPARATION OR TREATMENT, e.g. COOKING, MODIFICATION OF NUTRITIVE QUALITIES, PHYSICAL TREATMENT; PRESERVATION OF FOODS OR FOODSTUFFS, IN GENERAL
- A23L33/00—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof
- A23L33/10—Modifying nutritive qualities of foods; Dietetic products; Preparation or treatment thereof using additives
- A23L33/105—Plant extracts, their artificial duplicates or their derivatives
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/14—Fungi; Culture media therefor
- C12N1/145—Fungal isolates
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
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- C12N1/16—Yeasts; Culture media therefor
- C12N1/18—Baker's yeast; Brewer's yeast
- C12N1/185—Saccharomyces isolates
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
- C12P1/02—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes by using fungi
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23V—INDEXING SCHEME RELATING TO FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES AND LACTIC OR PROPIONIC ACID BACTERIA USED IN FOODSTUFFS OR FOOD PREPARATION
- A23V2002/00—Food compositions, function of food ingredients or processes for food or foodstuffs
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
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- C12R2001/645—Fungi ; Processes using fungi
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
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- C12R2001/66—Aspergillus
- C12R2001/685—Aspergillus niger
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/645—Fungi ; Processes using fungi
- C12R2001/66—Aspergillus
- C12R2001/69—Aspergillus oryzae
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/645—Fungi ; Processes using fungi
- C12R2001/79—Paecilomyces
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/645—Fungi ; Processes using fungi
- C12R2001/85—Saccharomyces
- C12R2001/865—Saccharomyces cerevisiae
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- Life Sciences & Earth Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Engineering & Computer Science (AREA)
- Health & Medical Sciences (AREA)
- Mycology (AREA)
- Microbiology (AREA)
- Zoology (AREA)
- Nutrition Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
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Abstract
The present invention discloses one kind and relieves the effect of alcohol compound preparation and preparation method thereof, pueraria lobata, clam extract are added in medical edible fungal fermentation process, fermented type compound preparation is made after homogeneous, spray drying after fermentation ends, can further develop the product forms such as chewable tablets, granule.The compound preparation is in good taste, and activity is high, has good dispelling effects of alcohol.
Description
Technical field
The invention belongs to field of health care food, and in particular to a kind of compound preparation and its preparation side with antialcoholism function
Method.
Background technology
In China, wine is not only a kind of time-honored drink, while can also be used as medicine.《The Yellow Emperor's Canon of Internal Medicine》In have many places to wine
Discussion, it is believed that wine is foodstuff essence, its property is agile and brave, drinks it in right amount, can fill Sheng passages through which vital energy circulates with harmonizing yingfen and weifen, the promoting flow of qi and blood circulation, beneficial
In body.However, more drinks can then trigger drunk, or even alcoholism.Such as《Health collection will》Institute's cloud:" wine person, the beneficial people of energy, also can
Speak sarcastically, save it and divide agent and drink it, a surname and hundred arteries and veins, disappear evil cold.If rising amount turns long, the mistake degree of drink, body gas makes weak, and spirit is invaded
It is dusk, preferably careful." with the raising of social standard of living, wine has become modern life food and drink and composition important in doings,
Since the disease ratio that excessive consumption of alcohol induces increases year by year, grave danger is caused to the health of human body.How to mitigate and drink not
When the infringement to human body, the problem of protecting the health of human body, become people's growing interest.Simple and effective, Small side effects are relieved the effect of alcohol
Medicine is also all the more favored.On this basis, the antialcoholic drug of integration of drinking and medicinal herbs raw material sources has obvious advantage.It not only meets
It is simple and effective, the requirement of Small side effects, and there is good nutritional supplementation.
Product category of sobering up currently on the market is various, and formulation is multifarious, but uses water extracting method mostly from Chinese herbal medicine
Middle extraction obtains, and contained active component content is not high, generally passes through one-side suppression ethanol absorption or accelerating alcohol metabolism
Blood alcohol concentration is reduced, its onset time of relieving the effect of alcohol is longer.Carrying out microbial fermentation to Chinese medicine and integration of drinking and medicinal herbs raw material can
Reacted with borrowing chemical composition complicated in enzyme system and Chinese medicine in microorganism, produce the active ingredient not having in some Chinese medicines
Or the content of some active ingredients is improved, reach drug effect enhancing, toxic side effect reduction, adaptability increase and other effects.
Relieved the effect of alcohol in Chinese Traditional Medicine, health-care sobering food development provides abundant foundation.Li Shizhen (1518-1593 A.D.)《Book on Chinese herbal medicine
Detailed outline》Upper record, clam have treatment " sore, furuncle poison, disperse accumulation block, relieving alcoholism " and other effects, illustrate that the software of marine source moves
Thing, such as clam, it is not only full of nutrition, and there is very high dietotherapy medical value, its a variety of amino acid being rich in, aliphatic acid,
Albumen etc. has good antialcoholism action.In addition,《This warp》Described in pueraria lobata can " control and quench one's thirst, high fever of the body, vomiting, all numbness, rise the moon
Gas, solves all poison ".《Prescriptions worth thousand gold》Described in pueraria lobata " control drunk do not wake up ".Modern study finds that pueraria lobata contains a large amount of osajins
Compound, wherein, Puerarin is the endemic element of pueraria lobata, can improve Green Tea Extract and brain work(by suppressing the release of 2 endorphins of β
Can, infringement of the lipid peroxidation to cell is reduced, has protective effect to acute alcoholism.According to traditional Chinese medicine theory and now
For Research foundation, microbe bi-directional fermentation is theoretical, instructs the exploitation of new hangover-alleviating health-care product.
The content of the invention
Relieve the effect of alcohol compound preparation and preparation method thereof the object of the present invention is to provide one kind.
The technical solution used in the present invention includes the following steps：(1) prepared by medical edible fungal seed liquor；(2) will prepare
Obtained medical edible fungal seed liquor, which is seeded in zymotic fluid, ferments；(3) after medical edible fungal ferments 4-10h, quality is added
The kudzu root extract of fraction 0.5-10%, continues the 12-72h that ferments；(4) mass fraction 1- is added into medical edible fungal zymotic fluid
20% clam extract, continues the 2-8h that ferments, obtains compound magma；(5) will be by the magma homogeneous after step (4) processing
Afterwards, spray drying obtains the compound preparation powder that relieves the effect of alcohol.
As the further scheme of the present invention：Medical edible fungal include but not limited to aspergillus niger, aspergillus oryzae, brewer's yeast,
Saccharomyces cerevisiae, Cordyceps militaris, cicada fungus, Paecilomyces hepiali chen, the one or more in Mortierella hepiali.
The preparation method of each medical edible fungal seed liquor is as follows in the present invention：
(1) preparation of aspergillus niger, aspergillus oryzae seed liquor：By the aspergillus niger of preservation or aspergillus oryzae strain in slant medium
Activate, transfer after being cultivated 4-6 days under the conditions of 30 DEG C and be once used as fermentation seed, by aspergillus niger or meter Qu through being made on inclined-plane
Mould slant pore sterile water wash, is poured into the physiological saline by sterilizing in advance, wherein tens of beades are pre-loaded with,
Spore is fully broken up in vibration, is made 1 × 108The spore suspension of a/mL, is aspergillus niger or aspergillus oryzae seed liquor.
(2) preparation of brewer's yeast, saccharomyces cerevisiae seed liquor：The barms of preservation is activated in slant medium,
Transfer after being cultivated 2-4 days under the conditions of 25-30 DEG C and be once used as fermentation seed, the yeast slant pore through being made on inclined-plane is used
Sterile water wash, is poured into the physiological saline by sterilizing in advance, wherein being pre-loaded with tens of beades, vibration is fully broken up
Spore, is made 1 × 108The spore suspension of a/mL, that is, obtain brewer's yeast or saccharomyces cerevisiae seed liquor.
(3) preparation of Cordyceps militaris, cicada fungus, Paecilomyces hepiali chen, Mortierella hepiali seed liquor：By each Cordyceps Militaris of preservation
Kind activates in slant medium, transfers after being cultivated 5-9 days under the conditions of 25-30 DEG C and is once used as fermentation seed, will be through inclined-plane
On the cordyceps sinensis slant pore sterile water wash that makes, pour into the physiological saline by sterilizing in advance, wherein being pre-loaded with counting
Ten beades, vibration fully break up spore, are made 1 × 108The spore suspension of a/mL, that is, obtain all kinds of Cordyceps Militaris seed liquors.
Each medical edible fungal culture medium used in the present invention is as follows：
(1) aspergillus niger, aspergillus oryzae seed slant medium are potato culture, and potato 200g, is boiled after adding water 500mL
30min is boiled, with filtered through gauze taking juice, adds glucose 20g, 15~20 grams of agar adds water to 1000mL, natural pH, 121 DEG C
High pressure steam sterilization 20min.Remove agar, the same slant medium of other compositions in fermentative medium formula；
(2) brewer's yeast seed slant medium is wheat juice culture medium, 12-15 Baume degrees, pH 5.1-5.5,121 DEG C of height
Press steam sterilizing 20min.Remove agar, the same slant medium of other compositions in fermentative medium formula
(3) saccharomyces cerevisiae seed slant medium is YPD culture mediums, glucose 2%, peptone 2%, dusty yeast 1%, fine jade
Fat 2%, distilled water are prepared, natural ph, 121 DEG C of high pressure steam sterilization 20min.Remove agar in fermentative medium formula, its
The same slant medium of his component
(4) all kinds of Cordyceps Militaris (Cordyceps militaris, cicada fungus, Paecilomyces hepiali chen, Mortierella hepiali) seed inclined-plane culture basigamys
Fang Wei, glucose 3%, peptone 1%, potassium dihydrogen phosphate 0.2%, anhydrous magnesium sulfate 0.1%, vitamin B1 0.01% are natural
PH, 121 DEG C of high pressure steam sterilization 20min.Remove agar, the same slant medium of other compositions in fermentative medium formula.
As the further scheme of the present invention：The medical edible fungal seed liquor being prepared is seeded in zymotic fluid and is fermented
Specific method be：
(1) aspergillus niger, aspergillus oryzae fermentation：By obtained spore suspension with 1 × 107The inoculum concentration of a/mL is trained by fermentation
Support in base, fermented and cultured 2-6 days；
(2) brewer's yeast, saccharomyces cerevisiae：By obtained spore suspension with 1 × 107The inoculum concentration of a/mL is trained by fermentation
Support in base, fermented and cultured 0.5-4 days；
(3) fermentation of Cordyceps militaris, cicada fungus, Paecilomyces hepiali chen, Mortierella hepiali：By obtained spore suspension with 1 ×
107The inoculum concentration of a/mL is by fermentation medium, fermented and cultured 2-8 days；
As the further scheme of the present invention：Pueraria lobata dry matter is smashed in pulverizer, 40 mesh sieves is crossed, according to solid-liquid ratio 1:
5-1:10 add deionized water, repeat extraction 2 times, merge supernatant, spray drying, obtains kudzu root extract.
As the further scheme of the present invention：Clam extract is using obtained by enzyme process assisted extraction.Clam software passes through
Add water and protease and obtain clam enzymolysis liquid, centrifuging and taking supernatant after destroy the enzyme treatment, spray drying obtains clam extract.Albumen
Enzyme includes but not limited to trypsase, acid protease, alkali protease, papain, bromelain, compound protein
One or more in enzyme, flavor protease, neutral proteinase, dosage 100-1000U/mL.
The beneficial effects of the invention are as follows：The compound preparation that relieves the effect of alcohol of the present invention, has no toxic side effect, and smell is fragrant, and taste is slightly sweet, mouthfeel
It is good；Various amino acid, the electrolytes and minerals easily absorbed containing human body；Active component content is high, green safe.
Figure of description
Fig. 1 differences compound that relieves the effect of alcohol combines influence to mouse formulation alcoholism
Fig. 2 compounds combine the dose-dependence of dispelling effects of alcohol
Embodiment
Following embodiments are used to be better understood from above-mentioned technical proposal, but protection scope of the present invention is not limited to following implementations
Example.
Embodiment 1 is relieved the effect of alcohol the preparation of compound preparation
First, compound preparation combination 1
1st, the preparation of aspergillus niger seed liquor：The aspergillus niger of preservation is activated in PDA slant mediums, is trained under the conditions of 30 DEG C
Transfer after supporting 4 days and be once used as fermentation seed, the aspergillus niger slant pore sterile water wash through being made on inclined-plane is poured into
In advance in the physiological saline by sterilizing, wherein being pre-loaded with tens of beades, spore is fully broken up in vibration, is made 1 × 108
The spore suspension of a/mL, obtains aspergillus niger seed liquor.
2nd, the preparation of kudzu root extract：Pueraria lobata dry matter is smashed in pulverizer, 40 mesh sieves is crossed, according to solid-liquid ratio 1:5 add
Enter deionized water, 60 DEG C of repetitions are extracted 2 times, merge supernatant, spray drying, obtains kudzu root extract.
3rd, the preparation of clam extraction：Solid-liquid ratio 1 is pressed after the homogenate of clam software:5 add water and mix, and are added according to 300U/mL
40 DEG C of acid protease enzymolysis 4h obtain clam enzymolysis liquid, and centrifuging and taking supernatant after destroy the enzyme treatment, spray drying obtains clam extraction
Thing.
4th, the fermentation of aspergillus niger：By obtained aspergillus niger spore suspension with 1 × 107The inoculum concentration of a/mL is seeded to fermentation
In culture medium, after fermentation culture for 2 days, 0.5% kudzu root extract is added, continue the 12h that ferments, add 20% clam extraction
Thing, continues the 8h that ferments.After fermentation, homogenization, spray drying obtain compound preparation combination 1.
2nd, compound preparation combination 2
1st, the preparation of saccharomyces cerevisiae seed liquor：The saccharomyces cerevisiae strain of preservation is activated in YPD slant mediums, 25 DEG C
Under the conditions of cultivate 3 days after transfer and be once used as fermentation seed, by the saccharomyces cerevisiae slant pore through being made on inclined-plane with sterile
Water cleans, and pours into the physiological saline by sterilizing in advance, wherein being pre-loaded with tens of beades, spore is fully broken up in vibration
Son, is made 1 × 108The spore suspension of a/mL, obtains saccharomyces cerevisiae seed liquor.
2nd, the preparation of kudzu root extract：Pueraria lobata dry matter is smashed in pulverizer, 40 mesh sieves is crossed, according to solid-liquid ratio 1:7 add
Enter deionized water, 80 DEG C of repetitions are extracted 2 times, merge supernatant, spray drying, obtains kudzu root extract.
3rd, the preparation of clam extraction：Solid-liquid ratio 1 is pressed after the homogenate of clam software:8 add water and mix, and are added according to 1000U/mL
40 DEG C of acid protease enzymolysis 2h obtain clam enzymolysis liquid, and centrifuging and taking supernatant after destroy the enzyme treatment, spray drying obtains clam extraction
Thing.
4th, the fermentation of saccharomyces cerevisiae：By obtained saccharomyces cerevisiae spore suspension with 1 × 107The inoculum concentration of a/mL is seeded to
In fermentation medium, after fermentation culture for 3 days, 5% kudzu root extract is added, continue the 36h that ferments, added 10% clam and carry
Thing is taken, continues the 4h that ferments.After fermentation, homogenization, spray drying obtain compound preparation combination 2.
3rd, compound preparation combination 3
1st, the preparation of Cordyceps militaris seed liquor：The Cordyceps militaris spawn of preservation is activated in slant medium, under the conditions of 30 DEG C
Culture transfers after 6 days and is once used as fermentation seed, and the Cordyceps militaris slant pore sterile water wash through being made on inclined-plane is fallen
Enter in the physiological saline by sterilizing in advance, wherein be pre-loaded with tens of beades, spore is fully broken up in vibration, it is made 1 ×
108The spore suspension of a/mL, obtains Cordyceps militaris seed liquor.
2nd, the preparation of kudzu root extract：Pueraria lobata dry matter is smashed in pulverizer, 40 mesh sieves is crossed, according to solid-liquid ratio 1:10 add
Enter deionized water, 100 DEG C of repetitions are extracted 2 times, merge supernatant, spray drying, obtains kudzu root extract.
3rd, the preparation of clam extraction：Solid-liquid ratio 1 is pressed after the homogenate of clam software:2 add water and mix, and are added according to 100U/mL
40 DEG C of papain enzymolysis 6h obtain clam enzymolysis liquid, and centrifuging and taking supernatant after destroy the enzyme treatment, spray drying obtains clam extraction
Thing.
4th, the fermentation of Cordyceps militaris：By obtained Cordyceps militaris spore suspension with 1 × 107The inoculum concentration of a/mL is seeded to fermentation
In culture medium, after fermentation culture for 7 days, 10% kudzu root extract is added, continue fermentation 8 days, add 1% clam extraction
Thing, continues the 2h that ferments.After fermentation, homogenization, spray drying obtain compound preparation combination 3.
The activity rating that relieves the effect of alcohol of 2 compound preparation of embodiment
First, different influences of the compound combination to mouse formulation alcoholism of relieving the effect of alcohol are compared
Kunming mice 40 is taken, weight 25-30g/ only, every group 10, is randomly divided into control group, 1 experimental group of combination, combination
2 experimental groups, 3 experimental groups of combination.Animal fasting for solids but not liquids 12h before experiment, control group are filled by the dosage of 0.10mL/ (10g weight)
Stomach physiological saline, gavage 56 after 30minoWhite wine 0.15mL/ (10g weight)；Compound preparation is first configured to concentration by experimental group
The suspension of 1g/mL, then according to the dosage gavage compound preparation of 0.10mL/ (10g weight), gavage 56 after 30minoWhite wine
0.15mL/ (10g weight).Eye socket is carried out to mouse in after drinking 30,60,90,120,180,240min respectively and takes hematometry blood
Concentration of alcohol.Ethanol in blood concentration is measured using Alcohol dehydrogenase method, is measured using ultraviolet specrophotometer in wavelength 340nm
The light absorption value at place, establishing criteria curve are calculated.
Testing result shows that each compound preparation combination there is good blood concentration of alcohol to reduce work acute alcoholism
With, and combine 2 and be substantially better than other combinations.Illustrate that there is good dispelling effects of alcohol using compound preparation produced by the present invention.
2nd, compound unitized dose dependence is investigated
Take kunming mice 40, weight 25-30g/ only, every group 10, be randomly divided into control group, low dosage experimental group, in
Dosage experiments group, high dose experimental group.Animal fasting for solids but not liquids 12h before experiment, control group press the agent of 0.10mL/ (10g weight)
Gavage physiological saline is measured, 56 ° of white wine 0.15mL/ of gavage (10g weight) after 30min.It is experimental drug with compound preparation combination 2,
Compound preparation is first configured to the suspension that concentration is 1g/mL, then respectively according to 0.05mL/ (10g weight), 0.10mL/
The dosage gavage compound preparation of (10g weight), 0.15mL/ (10g weight), 56 ° of white wine 0.15mL/ (10g bodies of gavage after 30min
Weight).Eye socket is carried out to mouse in after drinking 30,60,90,120,180,240min respectively and takes hematometry alcohol levels in blood samples concentration.Measure
Method is same as above.
It is found through experiments that, compound preparation has obvious dependence to acute alcoholism dispelling effects of alcohol, with dosage
Increase, blood concentration of alcohol reduce effect it is better.Illustrate that product of the present invention has good dispelling effects of alcohol.
Embodiment 3 is relieved the effect of alcohol the preparation of compound preparation chewable tablets
The compound preparation powder that relieves the effect of alcohol that compound preparation combination 2 is obtained, with pregelatinized starch, microcrystalline cellulose, stearic acid
Magnesium, silica are put into mixer and stir evenly, and soft material is made in the edible alcohol stirring for adding 5%-15%, is put into granulator
Pelletized with 20 mesh sieves, obtained particle is dried, collator whole grain is put into after particle drying, it is important according to 200mg pieces
Ask, carry out tabletting with tablet press machine and pack up to chewable tablets.
Embodiment 4 is relieved the effect of alcohol the preparation of compound preparation granule
The compound preparation powder that relieves the effect of alcohol that compound preparation combination 2 is obtained, is uniformly mixed with Icing Sugar, beta-cyclodextrin, adds 1-
10% edible alcohol, is pelletized using oscillating granulator, then is made up uniform of 10-20 mesh sieve nets with fashion of extrusion
Particle, then dry at 60-80 DEG C, the water content control of particle is sieving after 2% or so, wet grain drying, makes particle equal
It is even, composite aluminium plastic bag, and dry storage are sub-packed in using automatic granular packaging machine.
Claims (8)
- Compound preparation and preparation method thereof 1. one kind is relieved the effect of alcohol, it is characterised in that comprise the steps of：(1) prepared by medical edible fungal seed liquor；(2) the medical edible fungal seed liquor being prepared is seeded in zymotic fluid and fermented；(3) after medical edible fungal ferments 4-10h, the kudzu root extract of mass fraction 0.5-10% is added, continues the 12- that ferments 72h；(4) clam extract of mass fraction 1-20% is added into medical edible fungal zymotic fluid, continues the 2-8h that ferments, is answered Square magma；(5) will be after the magma homogeneous after step (4) processing, spray drying obtains the compound preparation powder that relieves the effect of alcohol.
- 2. according to the method described in claim 1, it is characterized in that, the medical edible fungal in the step (1) includes but unlimited One in aspergillus niger, aspergillus oryzae, brewer's yeast, saccharomyces cerevisiae, Cordyceps militaris, cicada fungus, Paecilomyces hepiali chen, Mortierella hepiali Kind is several；Specific method is：(1) aspergillus niger, rice by mould seed liquor preparation：The aspergillus niger of preservation or rice are lived by mould species in slant medium Change, transfer after being cultivated 4-6 days under the conditions of 30 DEG C and be once used as fermentation seed, by the aspergillus niger through being made on inclined-plane or rice by mould Slant pore sterile water wash, pours into the physiological saline by sterilizing in advance, wherein being pre-loaded with tens of beades, shakes Swing and fully break up spore, be made 1 × 108The spore suspension of a/mL, is aspergillus niger or aspergillus oryzae seed liquor；(2) preparation of brewer's yeast, saccharomyces cerevisiae seed liquor：The barms of preservation is activated in slant medium, 25-30 Transfer after being cultivated 2-4 days under the conditions of DEG C and be once used as fermentation seed, by the yeast slant pore through being made on inclined-plane with sterile Water cleans, and pours into the physiological saline by sterilizing in advance, wherein being pre-loaded with tens of beades, spore is fully broken up in vibration Son, is made 1 × 108The spore suspension of a/mL, that is, obtain brewer's yeast or saccharomyces cerevisiae seed liquor；(3) preparation of Cordyceps militaris, cicada fungus, Paecilomyces hepiali chen, Mortierella hepiali seed liquor：Each cordyceps species of preservation are existed Activated in slant medium, transfer after being cultivated 5-9 days under the conditions of 25-30 DEG C and be once used as fermentation seed, will be through being made on inclined-plane Good cordyceps sinensis slant pore sterile water wash, is poured into the physiological saline by sterilizing in advance, wherein being pre-loaded with tens of grains Bead, vibration fully break up spore, are made 1 × 108The spore suspension of a/mL, that is, obtain all kinds of Cordyceps Militaris seed liquors.
- 3. according to the method described in claim 2, it is characterized in that, seed slant medium is Ma Ling in the method (1) Potato culture medium, potato 200g, boils 30min after adding water 500mL, with filtered through gauze taking juice, adds glucose 20g, agar 15~20 grams, add water to 1000mL, natural pH, 121 DEG C of high pressure steam sterilization 20min；Fermentative medium formula is inclined-plane culture Remove agar, the same slant medium of other compositions in based formulas；Brewer's yeast seed seed inclined-plane culture in the method (2) Base is wheat juice culture medium, 12-15 Baume degrees, pH 5.1-5.5,121 DEG C of high pressure steam sterilization 20min.Fermentative medium formula In remove agar, the same slant medium of other compositions；Saccharomyces cerevisiae seed culture medium is YPD culture mediums in the method (2), Glucose 2%, peptone 2%, dusty yeast 1%, agar 2%, distilled water are prepared, natural ph, 121 DEG C of high pressure steam sterilizations 20min；Fermentative medium formula is to remove agar, the same slant medium of other compositions in inclined-plane culture based formulas；The side All kinds of Cordyceps Militaris seed slant mediums are glucose 3% in method (3), peptone 1%, potassium dihydrogen phosphate 0.2%, anhydrous slufuric acid Magnesium 0.1%, vitamin B1 0.01%, natural pH, 121 DEG C of high pressure steam sterilization 20min；Fermentative medium formula is trained for inclined-plane Support in based formulas and remove agar, the same slant medium of other compositions.
- 4. the according to the method described in claim 1, it is characterized in that, medical edible fungal that will be prepared in the step (2) Seed liquor is seeded to the specific method to ferment in zymotic fluid：(1) aspergillus niger, rice are by mould fermentation：By obtained spore suspension with 1 × 107The inoculum concentration of a/mL is seeded to fermentation medium In, fermented and cultured 2-6 days；(2) brewer's yeast, saccharomyces cerevisiae：By obtained spore suspension with 1 × 107The inoculum concentration of a/mL is seeded to fermentation medium In, fermented and cultured 0.5-4 days；(3) fermentation of Cordyceps militaris, cicada fungus, Paecilomyces hepiali chen, Mortierella hepiali：By obtained spore suspension with 1 × 107A/ The inoculum concentration of mL is seeded in fermentation medium, fermented and cultured 2-8 days.
- 5. according to the method described in claim 1, it is characterized in that, the kudzu root extract preparation method in the step (3) has Body is that pueraria lobata dry matter is smashed in pulverizer, 40 mesh sieves is crossed, according to solid-liquid ratio 1:5-1:10 add deionized water, 60-100 DEG C repetition extraction 2 times, merge supernatant, spray drying, obtain kudzu root extract.
- 6. according to the method described in claim 1, it is characterized in that, the clam extract in the step (3) is using enzyme process Obtained by assisted extraction.Specifically, by adding water and protease obtains clam enzymolysis liquid after the homogenate of clam software, after destroy the enzyme treatment Centrifuging and taking supernatant, spray drying obtain clam extract.
- 7. according to claim 6, protease includes but not limited to trypsase, acid protease, alkali protease, pawpaw One or more in protease, bromelain, compound protease, flavor protease, neutral proteinase, dosage 100- 1000U/mL。
- 8. one kind is relieved the effect of alcohol compound preparation and preparation method thereof according to claim 1, it is characterised in that can be made into including but not It is limited to the formal products such as chewable tablets, granule, supensoid agent.
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|---|---|---|---|---|
| CN111471859A (en) * | 2020-03-25 | 2020-07-31 | 南华大学 | Method for leaching uranium from low-grade uranium ore by macro-particle reinforced aspergillus niger |
| CN111471859B (en) * | 2020-03-25 | 2021-08-20 | 南华大学 | Method for leaching uranium from low-grade uranium ore by macro-particle reinforced aspergillus niger |
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