CN107896550A - A kind of bletilla striata seeds processing method - Google Patents
A kind of bletilla striata seeds processing method Download PDFInfo
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- CN107896550A CN107896550A CN201711002654.8A CN201711002654A CN107896550A CN 107896550 A CN107896550 A CN 107896550A CN 201711002654 A CN201711002654 A CN 201711002654A CN 107896550 A CN107896550 A CN 107896550A
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- bletilla striata
- starch
- striata seeds
- processing method
- starch milk
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
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- Coloring Foods And Improving Nutritive Qualities (AREA)
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Abstract
The invention discloses a kind of bletilla striata seeds processing method, it is related to Chinese medicine plant technical field, comprises the following steps：（1）Prepare starch milk；（2）The bletilla striata is handled；（3）Starch milk is mixed with bletilla striata seeds；（4）Liquor natrii hypochloritis is handled；The present invention is by preparing starch milk, bletilla striata seeds are mixed with starch milk, then certain time is handled in alkaline conditions again, the permeability of seed can be improved to a certain extent, while handled again using Q-enzyrne afterwards, then processing is modified to starch, while starch is modified, a certain amount of active material can be produced, the development of bletilla striata seeds embryo can be promoted to a certain extent.
Description
Technical field
The invention belongs to Chinese medicine plant technical field, and in particular to a kind of bletilla striata seeds processing method.
Background technology
The bletilla striata, (scientific name：Bletilla striata) also known as Lian Jicao, Gan Gen, give free of charge, is indocalamus orchid, Zhu Lan, purple blue, purple
A species of orchid, hundred large bamboo hats with a conical crown and broad brim.Perennial herb bulbous plant (root tuber), plant are 18-60 centimetres high.It is mainly distributed on China, Japan and Burma
It is northern.The main florescence may all bloom in spring, but according to the difference of various regions weather, late Winter Solstice early summer.The bletilla striata has extensive medicinal
Value and Ornamental value.It is mainly used for astringing to arrest bleeding, detumescence and promoting granulation.Flower has the colors such as purplish red, white, blue, yellow and powder.
Existing method plants the bletilla striata, it is necessary to which bletilla striata seeds are carried out with certain processing to improve the germination percentage of bletilla striata seeds,
But the germination percentage for improving bletilla striata seeds by a small margin is merely capable of using the clear water immersion bletilla striata seeds of certain temperature merely,
The market demand can not be met by improving effect, meanwhile, it does not have facilitation effect to the seedlings root germination after bletilla striata germination.
The content of the invention
The purpose of the present invention is for it is existing the problem of, there is provided a kind of bletilla striata seeds processing method.
The present invention is achieved by the following technical solutions：
A kind of bletilla striata seeds processing method, comprises the following steps
（1）Starch dispersion is obtained to the starch milk that mass fraction is 15.5-18.0% in water, starch milk is heated to 90-95 DEG C
When insulation 2-4 is small, 35 DEG C are then naturally cooled to；
（2）Bletilla striata seeds are put into mass fraction to soak 30-40min in 80% ethanol solution, are then taken out, it is clear using clear water
Wash, naturally dry；
（3）By step（2）The bletilla striata seeds of processing press 120-150g：The ratio and step of 250-300mL（1）In starch milk mix
Close, 15-20min is stirred under 120r/min rotating speeds, then adjust pH to 8.2-8.8, stand 15min, then by every gram of starch
The ratio of 520-550U adds Q-enzyrne into starch milk, adjusts the temperature to 35-40 DEG C, isothermal holding 5-8h, obtains white
Splendid achnatherum seed and modified starch mixture；
（4）The liquor natrii hypochloritis of its quality 10.2-10.5% is added into bletilla striata seeds and modified starch mixture, with 500r/
When min rotating speeds stirring 2 is small, when then standing 1 is small, bletilla striata seeds is filtered, is cleaned to neutrality using clear water, is then manually urged
Bud, you can.
Further, the step（1）Middle starch is cornstarch and Semen Castaneae starch mixture, wherein cornstarch and chestnut
Sub- starch quality ratio is 9.5:1.
Further, the step（1）Middle water is deionized water.
Further, the step（2）It is 200g that middle bletilla striata seeds soak mixing quality ratio in ethanol solution：400mL.
Further, the step（3）Middle adjusting pH to 8.2-8.8 be use mass fraction for 15% ammonia spirit pair
Starch milk carries out pH adjustings.
Further, the step（3）Middle liquor natrii hypochloritis's mass concentration is 0.15mol/L.
Further, the step（4）Middle artificial germination is in 30 DEG C of temperature, in the environment of relative air humidity is 75%
Vernalization is carried out, vernalization to more than 60% seed shows money or valuables one carries unintentionally.
The present invention has the following advantages compared with prior art：The present invention is by preparing starch milk, by bletilla striata seeds and starch
Breast is mixed, and then handles certain time in alkaline conditions again, can improve the permeability of seed to a certain extent, afterwards
While handled again using Q-enzyrne, then processing is modified to starch, while starch is modified, one can be produced
Quantitative active material, can promote the development of bletilla striata seeds embryo to a certain extent, then add liquor natrii hypochloritis again, secondary
Sodium chlorate has strong oxidizing property, can active oxide material, germination period is enabled to by the synergistic effect of itself and sodium hypochlorite
Bletilla striata seeds endoperoxides hydrogenase activity, Acid Phosphatase Activity and amount of ATP significantly improve, and peroxidating
The height of hydrogenase activity is positively correlated with the vigor of seed, by improving the activity of catalase come raw in bletilla striata seeds
Reason metabolism is more vigorous, and Acid Phosphatase Activity gradually increases with the sprouting process of seed, its active height is shown
The speed that seed is sprouted, by improving Acid Phosphatase Activity, to accelerate the sprouting of bletilla striata seeds, so as to improve bletilla striata seeds
Germination percentage, and the growth to bletilla striata seedling lateral root has significant facilitation.
Embodiment
Embodiment 1
A kind of bletilla striata seeds processing method, comprises the following steps
（1）Starch dispersion is obtained to the starch milk that mass fraction is 15.5% in water, it is small that starch milk is heated to 90 DEG C of insulations 2
When, then naturally cool to 35 DEG C；
（2）Bletilla striata seeds are put into mass fraction to soak 30min in 80% ethanol solution, then takes out, is cleaned using clear water,
Naturally dry；
（3）By step（2）The bletilla striata seeds of processing press 120g：The ratio and step of 250mL（1）In starch milk mixing,
15min is stirred under 120r/min rotating speeds, then adjusts pH to 8.2, stands 15min, then in every gram of starch 520U ratio to shallow lake
Q-enzyrne is added in powder breast, adjusts the temperature to 35 DEG C, isothermal holding 5h, obtains bletilla striata seeds and modified starch mixture；
（4）The liquor natrii hypochloritis of its quality 10.2% is added into bletilla striata seeds and modified starch mixture, is turned with 500r/min
When speed stirring 2 is small, when then standing 1 is small, bletilla striata seeds is filtered, is cleaned using clear water to neutrality, then carries out artificial germination, i.e.,
Can.
Further, the step（1）Middle starch is cornstarch and Semen Castaneae starch mixture, wherein cornstarch and chestnut
Sub- starch quality ratio is 9.5:1.
Further, the step（1）Middle water is deionized water.
Further, the step（2）It is 200g that middle bletilla striata seeds soak mixing quality ratio in ethanol solution：400mL.
Further, the step（3）Middle adjusting pH to 8.2 is 15% ammonia spirit to starch to use mass fraction
Breast carries out pH adjustings.
Further, the step（3）Middle liquor natrii hypochloritis's mass concentration is 0.15mol/L.
Further, the step（4）Middle artificial germination is in 30 DEG C of temperature, in the environment of relative air humidity is 75%
Vernalization is carried out, vernalization to more than 60% seed shows money or valuables one carries unintentionally.
Embodiment 2
A kind of bletilla striata seeds processing method, comprises the following steps
（1）Starch dispersion is obtained to the starch milk that mass fraction is 18.0% in water, it is small that starch milk is heated to 95 DEG C of insulations 4
When, then naturally cool to 35 DEG C；
（2）Bletilla striata seeds are put into mass fraction to soak 40min in 80% ethanol solution, then takes out, is cleaned using clear water,
Naturally dry；
（3）By step（2）The bletilla striata seeds of processing press 150g：The ratio and step of 300mL（1）In starch milk mixing,
20min is stirred under 120r/min rotating speeds, then adjusts pH to 8.8, stands 15min, then the ratio in every gram of starch 520-550U
Q-enzyrne is added into starch milk, adjusts the temperature to 40 DEG C, isothermal holding 8h, obtains bletilla striata seeds and mixed with modified starch
Thing；
（4）The liquor natrii hypochloritis of its quality 10.5% is added into bletilla striata seeds and modified starch mixture, is turned with 500r/min
When speed stirring 2 is small, when then standing 1 is small, bletilla striata seeds is filtered, is cleaned using clear water to neutrality, then carries out artificial germination, i.e.,
Can.
Further, the step（1）Middle starch is cornstarch and Semen Castaneae starch mixture, wherein cornstarch and chestnut
Sub- starch quality ratio is 9.5:1.
Further, the step（1）Middle water is deionized water.
Further, the step（2）It is 200g that middle bletilla striata seeds soak mixing quality ratio in ethanol solution：400mL.
Further, the step（3）Middle adjusting pH to 8.8 is 15% ammonia spirit to starch to use mass fraction
Breast carries out pH adjustings.
Further, the step（3）Middle liquor natrii hypochloritis's mass concentration is 0.15mol/L.
Further, the step（4）Middle artificial germination is in 30 DEG C of temperature, in the environment of relative air humidity is 75%
Vernalization is carried out, vernalization to more than 60% seed shows money or valuables one carries unintentionally.
Embodiment 3
A kind of bletilla striata seeds processing method, comprises the following steps
（1）Starch dispersion is obtained to the starch milk that mass fraction is 16.0% in water, it is small that starch milk is heated to 92 DEG C of insulations 3
When, then naturally cool to 35 DEG C；
（2）Bletilla striata seeds are put into mass fraction to soak 35min in 80% ethanol solution, then takes out, is cleaned using clear water,
Naturally dry；
（3）By step（2）The bletilla striata seeds of processing press 130g：The ratio and step of 280mL（1）In starch milk mixing,
18min is stirred under 120r/min rotating speeds, then adjusts pH to 8.5, stands 15min, then in every gram of starch 530U ratio to shallow lake
Q-enzyrne is added in powder breast, adjusts the temperature to 35-40 DEG C, isothermal holding 5-8h, bletilla striata seeds is obtained and is mixed with modified starch
Compound；
（4）The liquor natrii hypochloritis of its quality 10.3% is added into bletilla striata seeds and modified starch mixture, is turned with 500r/min
When speed stirring 2 is small, when then standing 1 is small, bletilla striata seeds is filtered, is cleaned using clear water to neutrality, then carries out artificial germination, i.e.,
Can.
Further, the step（1）Middle starch is cornstarch and Semen Castaneae starch mixture, wherein cornstarch and chestnut
Sub- starch quality ratio is 9.5:1.
Further, the step（1）Middle water is deionized water.
Further, the step（2）It is 200g that middle bletilla striata seeds soak mixing quality ratio in ethanol solution：400mL.
Further, the step（3）Middle adjusting pH to 8.5 is 15% ammonia spirit to starch to use mass fraction
Breast carries out pH adjustings.
Further, the step（3）Middle liquor natrii hypochloritis's mass concentration is 0.15mol/L.
Further, the step（4）Middle artificial germination is in 30 DEG C of temperature, in the environment of relative air humidity is 75%
Vernalization is carried out, vernalization to more than 60% seed shows money or valuables one carries unintentionally.
Comparative example 1：Starch is differed only in only with cornstarch with embodiment 1.
Comparative example 2：Differed only in embodiment 1 without step（4）Processing.
By the same batch of bletilla striata seeds that mass of 1000 kernel is 20.12g, be randomly divided into 5 groups, every group 200, be respectively adopted embodiment with
Comparative example method is handled, and is then seeded into nursery, reference《International seed inspection procedure》Formula calculate germination percentage and
Germinating energy；
Germination percentage（%）=germination number/experiment seed number × 100%；
Germinating energy（%）Most germination number/experiment seed number × 100% of=germination process same day germination；
Table 1
| Germination percentage % | Germinating energy % | |
| Embodiment 1 | 93 | 135 |
| Embodiment 2 | 94 | 140 |
| Embodiment 3 | 91 | 130 |
| Comparative example 1 | 88 | 102 |
| Comparative example 2 | 82 | 72 |
As can be seen from Table 1, the method for the present invention processing bletilla striata seeds can effectively improve germination percentage and the germination of bletilla striata seeds
Gesture.
Claims (7)
1. a kind of bletilla striata seeds processing method, it is characterised in that comprise the following steps
（1）Starch dispersion is obtained to the starch milk that mass fraction is 15.5-18.0% in water, starch milk is heated to 90-95 DEG C
When insulation 2-4 is small, 35 DEG C are then naturally cooled to；
（2）Bletilla striata seeds are put into mass fraction to soak 30-40min in 80% ethanol solution, are then taken out, it is clear using clear water
Wash, naturally dry；
（3）By step（2）The bletilla striata seeds of processing press 120-150g：The ratio and step of 250-300mL（1）In starch milk mix
Close, 15-20min is stirred under 120r/min rotating speeds, then adjust pH to 8.2-8.8, stand 15min, then by every gram of starch
The ratio of 520-550U adds Q-enzyrne into starch milk, adjusts the temperature to 35-40 DEG C, isothermal holding 5-8h, obtains white
Splendid achnatherum seed and modified starch mixture；
（4）The liquor natrii hypochloritis of its quality 10.2-10.5% is added into bletilla striata seeds and modified starch mixture, with 500r/
When min rotating speeds stirring 2 is small, when then standing 1 is small, bletilla striata seeds is filtered, is cleaned to neutrality using clear water, is then manually urged
Bud, you can.
A kind of 2. bletilla striata seeds processing method according to claim 1, it is characterised in that the step（1）Middle starch is
Cornstarch and Semen Castaneae starch mixture, wherein cornstarch are 9.5 with Semen Castaneae starch mass ratio:1.
A kind of 3. bletilla striata seeds processing method according to claim 1, it is characterised in that the step（1）Middle water is to go
Ionized water.
A kind of 4. bletilla striata seeds processing method according to claim 1, it is characterised in that the step（2）Middle bletilla seed
It is 200g that son soaks mixing quality ratio in ethanol solution：400mL.
A kind of 5. bletilla striata seeds processing method according to claim 1, it is characterised in that the step（3）Middle adjusting pH
It is to use mass fraction to carry out pH adjustings to starch milk for 15% ammonia spirit to 8.2-8.8.
A kind of 6. bletilla striata seeds processing method according to claim 1, it is characterised in that the step（3）Middle hypochlorous acid
Sodium solution mass concentration is 0.15mol/L.
A kind of 7. bletilla striata seeds processing method according to claim 1, it is characterised in that the step（4）In manually urge
Bud is in 30 DEG C of temperature, and relative air humidity carries out vernalization in the environment of being 75%, and vernalization to more than 60% seed shows money or valuables one carries unintentionally.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201711002654.8A CN107896550A (en) | 2017-10-24 | 2017-10-24 | A kind of bletilla striata seeds processing method |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711002654.8A CN107896550A (en) | 2017-10-24 | 2017-10-24 | A kind of bletilla striata seeds processing method |
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| Publication Number | Publication Date |
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| CN107896550A true CN107896550A (en) | 2018-04-13 |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108738469A (en) * | 2018-05-24 | 2018-11-06 | 界首市龙源种植专业合作社 | A kind of processing method improving bletilla striata seeds germination percentage |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105210875A (en) * | 2015-10-15 | 2016-01-06 | 铜仁市金农绿色农业科技有限公司 | A kind of bletilla striata quick propagation method |
| CN105325287A (en) * | 2015-11-24 | 2016-02-17 | 长江大学 | Disinfection and inoculation method of microphyte seeds and pollen |
| CN106068790A (en) * | 2016-07-19 | 2016-11-09 | 江苏省中国科学院植物研究所 | A kind of method of bletilla striata seeds direct sowing and seedling |
| CN106946610A (en) * | 2017-03-28 | 2017-07-14 | 云南恩润生物科技发展有限公司 | Bletilla striata nutrition endosperm and preparation method thereof |
-
2017
- 2017-10-24 CN CN201711002654.8A patent/CN107896550A/en not_active Withdrawn
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105210875A (en) * | 2015-10-15 | 2016-01-06 | 铜仁市金农绿色农业科技有限公司 | A kind of bletilla striata quick propagation method |
| CN105325287A (en) * | 2015-11-24 | 2016-02-17 | 长江大学 | Disinfection and inoculation method of microphyte seeds and pollen |
| CN106068790A (en) * | 2016-07-19 | 2016-11-09 | 江苏省中国科学院植物研究所 | A kind of method of bletilla striata seeds direct sowing and seedling |
| CN106946610A (en) * | 2017-03-28 | 2017-07-14 | 云南恩润生物科技发展有限公司 | Bletilla striata nutrition endosperm and preparation method thereof |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN108738469A (en) * | 2018-05-24 | 2018-11-06 | 界首市龙源种植专业合作社 | A kind of processing method improving bletilla striata seeds germination percentage |
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Application publication date: 20180413 |
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