CN107875093A - A kind of anti-aging skin repairs liquid and preparation method thereof - Google Patents
A kind of anti-aging skin repairs liquid and preparation method thereof Download PDFInfo
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- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
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- A61K8/30—Cosmetics or similar toiletry preparations characterised by the composition containing organic compounds
- A61K8/64—Proteins; Peptides; Derivatives or degradation products thereof
- A61K8/65—Collagen; Gelatin; Keratin; Derivatives or degradation products thereof
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Abstract
The invention belongs to skin care field, and in particular to a kind of anti-aging skin repairs liquid and preparation method thereof.The reparation liquid is made up of the component of following mass fraction:EGF 5.0 × 10‑3~1.0 × 10‑2%, the plant extracts 10~20% through electric field treatment, collagen 3~6%, nonapeptide 1 0.01~0.05%, acetyl group octapeptide 3 0.01~0.05%, stabilizer 1~3%, glycerine 10~22%, Sodium Hyaluronate 0.05~1.5% and water surplus.Skin repair liquid energy pole of the present invention significantly increases the elasticity, glossiness and wettability of skin, has significant anti-aging effects, and its stable system, it is efficacy lasting, importantly, using when be not in slight tingling sensation, improve the usage experience of consumer.
Description
Technical field
The invention belongs to skin care field, and in particular to a kind of anti-aging skin repairs liquid and preparation method thereof.
Background technology
With advancing age, fibroblast quantity gradually decreases, synthesizes collagen and bullet in the skin corium of skin
Property albumen ability decline, epidermal cell renewal slow down, barrier function weaken, cutin formed vigor decline, then result in skin
Skin is dry, the features such as wrinkle occurs.
There are some researches show, it is believed that the aging of skin is relevant with telomere aging.The gene of biology are determined by telomere program
Fixed fissional number, as cell division telomere is gradually shortened, is as short as then starting stopping heading signal to a certain degree, normally
Body cell senesce death.It is currently used method by promoting cell differentiation propagation to delay telomere aging, typically
Conventional active component has:1. biological agent class:Such as EGF, placenta extracting liquid, oligomeric polypeptide (such as peptide, flesh
Peptide, thymopeptide-5) etc.;2. natural galenical class, such as fleece-flower root, bluish dogbane, sealwort, pearl, radix polygonati officinalis, white fungus;3. chemistry
Synthesis, semi-synthetic preparation class:Such as vitamin A acid and its derivative, polyunsaturated fatty acid (DHA, EPA).Such as in Chinese patent Shen
Please in CN106109362A, inventor by Chinese herbaceous peony sead extract, lupin extract and lotus extract compounding use, from
And effectively can prevent and improve skin aging, and can firm skin, and play a part of anti aging effect;Such as Chinese special
In profit application CN103948535A, black bearberry, beech mast, polygoni cuspidati,radix, rosemary and radix glycyrrhizae are prepared into plant and evaporated by inventor
Go out liquid, then add micromolecular collagen, Co-Q10, EGF, the emulsifying agent of Argireline sum and be sufficiently mixed, shape
Into with anti-aging emulsion.
Such anti-apolexis composition also has many, but the based composition has some general character, is difficult to be overcome
The problem of:First, containing various active composition, such as biological species and the preparation of natural plants active component, active component is difficult to grow
Phase keeps activity, causes effect not lasting;Second, although the advantages of plant extracts has natural, safety, and effect is good,
Most of natural plants, particularly some uncommon plants can not be ignored to the excitant of skin, and most consumers are anti-
Slight local tingling sensation can be had by reflecting the skin when having used the anti-senile preparation of the extract containing various plants, if especially
When skin surface has wound, stimulate pain strong, and above-mentioned two problems, not yet effectively solved at present.
Therefore, for technical problem present in above-mentioned prior art, it is necessary to propose that a kind of anti-aging effects are good, simultaneously
It is efficacy lasting, in the absence of the composition of tingling sensation during use.
The content of the invention
The present invention is intended to provide a kind of anti-aging skin repairs liquid and preparation method thereof, specifically, the reparation liquid product
Matter is stable, efficacy lasting, and in use, will not produce tingling sensation and sense of discomfort.
In order to achieve the above object, the present invention uses following technical scheme:A kind of anti-aging skin repairs liquid, by following matter
The component of amount fraction is made:EGF 5.0 × 10-3~1.0 × 10-2%, the plant extracts 10 through electric field treatment~
20%th, collagen 3~6%, nonapeptide -1 0.01~0.05%, acetyl group octapeptide -30.01~0.05%, stabilizer 1~
3%th, glycerine 10~22%, Sodium Hyaluronate 0.05~1.5% and water surplus.
Further, the reparation liquid is made up of the component of following mass fraction:EGF 6.0 × 10-3~1.0
×10-2%, the plant extracts 15~20% through electric field treatment, collagen 3~5%, nonapeptide -10.01~0.03%, acetyl
More than base octapeptide -3 0.01~0.03%, stabilizer 1~3%, glycerine 10~20%, Sodium Hyaluronate 0.05~1.0% and water
Amount.
Further, the reparation liquid is made up of the component of following mass fraction:EGF 8.0 × 10-3%, warp
The plant extracts 15% of electric field treatment, collagen 3.5%, nonapeptide -1 0.02%, acetyl group octapeptide -3 0.02%, stably
Agent 1.5%, glycerine 15%, Sodium Hyaluronate 0.05% and water surplus.
Further, the plant extracts through electric field treatment is made by following steps:
S1, aloe extract preparation:Fresh aloe is taken, is cleaned, is dried, squeeze the juice, filters, obtains aloe extract;
S2, cordyceps sinensis flower extract preparation:Fresh cordyceps flower is taken, cleans, dries, adds 3~6 times of amounts of cordyceps flower weight
Decocting is boiled 1~3 time, and 30~60min, filtering, takes filtrate, produce every time;
S3, varying strength electric field treatment:Voltage is exposed to after above-mentioned aloe extract and cordyceps sinensis flower extract are mixed
10~23V, in 8~10v/m of electric-field intensity electric field after 1~3min, electric-field intensity is adjusted to 3~8v/m, continues with 20
~45s, is produced.
Further, be exposed to 10~20V, electricity after aloe extract and cordyceps sinensis flower extract being mixed in the step S2
In 8~10v/m of field intensity electric field after 1~3min, electric-field intensity is adjusted to 5~8v/m, handles 20~50s.
Further, the stabilizer is comprising 100~1000ppm Tween-80s degradation product and 10~100ppm parents
The aqueous solution of water amino acid.
Further, the Tween-80 degradation product is made by following steps:
Tween-80 solution is added in toward 100ml shaking flasks, 2~5wt% of inoculation concentration is 800~1000cfu/mL's
Trichoderma viride, it is 30~35 DEG C, rotating speed 120r/min to set temperature, cultivates 15d, centrifugation, takes supernatant;Supernatant is carried out successively
Micro-filtration, cation exchange and sterilizing, Tween-80 degradation product is made.
Further, the one kind of the hydrophilic amino acid in Glu, serine and glycine.
Further, the hydrophilic amino acid is selected from Glu.
Correspondingly, present invention also offers a kind of method for preparing above-mentioned skin repair composition, comprise the following steps:
Glycerine and stabilizer are mixed, are vortexed after mixing, adds EGF, the plant extract through electric field treatment
Thing, collagen, nonapeptide -1, acetyl group octapeptide -3, Sodium Hyaluronate and water, constantly it is vortexed and forms it into the molten of stable homogeneous
Liquid, sterilize, packaging, produce.
Plant extracts of the present invention is prepared using aloe and cordyceps flower as raw material, but because the juice of aloe is in microscope
Lower observation is in sharp keen crystalline solid, and therefore, the stimulation when in use to skin is larger, when user's skin is more sensitive, is led to
It can often feel slight tingling sensation be present.For the problem, inventor sends out finally by substantial amounts of experiment and creative work
It is existing, if its excitant to skin can be significantly reduced by carrying out electric field treatment to plant extracts, and if it is surprisingly possible that
The electric field treatment of varying strength is carried out, the long-time stability of extract can also be significantly improved.And the mechanism of the effect above is obtained,
Not yet clear, inventor, which thinks, is probably, and the processing of electric field makes the crystal structure of aloe juice be crystallized there occurs certain change
The sharp keen degree of body is significantly reduced compared with before processing, and therefore, the excitant of skin is reduced.What deserves to be explained is the voltage of electric field
Also there is large effect to treatment effect with current strength, and this influence is that milli is irregular, and therefore, inventor is through excessive
Amount experiment is drawn finally, is first 10~23V in voltage, electric-field intensity is to be placed in again under 3~8v/m intensity after 8~10v/m is handled
Processing, within the range, the influence for being all that the processing of electric field is brought, such as reduce stimulation of the plant extracts to skin
Property, and its active ingredient will not be impacted, applicant thinks to achieve unexpected technique effect.
Reparation liquid provided by the invention also contains collagen, nonapeptide -1 and acetyl in addition to plant extracts, growth factor
Base octapeptide -3, although each component collaboration has significant repair to steroid dependent dermatitis, polypeptide it is mutually interpolymerized
The activity decrease of collection phenomenon and growth factor is still the individual difficulty for being difficult to overcome.Thus, present invention also offers a kind of new
Stabilizer, it is the aqueous solution comprising 100~1000ppm Tween-80s degradation product and 10~100ppm hydrophilic amino acids.
It is demonstrated experimentally that stabilizer of the present invention can not only suppress the mutual aggregation of polypeptide, additionally it is possible to have the function that to stablize growth factor.
This point from test example two of the present invention as can be seen that add the skin repair composition of stabilizer of the present invention -4 DEG C, 20 DEG C with
And under conditions of normal temperature after storage 30d, the content of EGF has compared with blank control group (not containing stabilizer)
It is extremely significant to rise, meanwhile, the skin repair composition preserves under conditions of 25 ± 2 DEG C of temperature, relative humidity 60 ± 10%
Still clarified after 12 months.And it is worth noting that, it can also be seen that stabiliser content or the small change of component from experiment
Can cause can not expected effect, inventor thinks that stabilizer of the present invention composition achieves unexpected technique effect.
The present invention has advantages below:
1) the invention provides a kind of anti-aging skin to repair liquid, and it includes various active composition, and each active component phase
Interaction, have the function that Synergistic, there is significant anti-aging effects, and its stable system, it is efficacy lasting.
2) present invention to plant extracts carry out varying strength electric field treatment, significantly reduce sensitive user using when deposit
In the possibility of minimal irritation pain, the usage experience of consumer is improved.
3) present invention is by adding stabilizer, overcomes the mutual aggregation of polypeptide, the problem of growth factor activity declines, shows
The shelf-life for extending and repairing liquid is write, achieves significant progress.
Embodiment
The embodiment of form by the following examples, the above of the present invention is made further specifically
It is bright.But the scope that this should not be interpreted as to the above-mentioned theme of the present invention is only limitted to following examples.
The preparation of embodiment 1, Tween-80 degradation product
Tween-80 solution is added in toward 100ml shaking flasks, is inoculated with the Trichoderma viride that 3wt% concentration is 1000cfu/mL,
It is 35 DEG C, rotating speed 120r/min to set temperature, cultivates 15d, centrifugation, takes supernatant;Micro-filtration, 732 types sun are carried out to supernatant successively
Ion exchange carries out cation exchange and sterilizing, and Tween-80 degradation product is made.
Embodiment 2, a kind of anti-aging skin repair liquid
It is made up of the component of following mass fraction:EGF 8.0 × 10-3%, the plant extract through electric field treatment
Thing 15%, collagen 3.5%, nonapeptide -1 0.02%, acetyl group octapeptide -3 0.02%, stabilizer 1.5%, glycerine 15%,
Sodium Hyaluronate 0.05% and water surplus, wherein stabilizer are containing the Tween-80 degraded described in 500ppm embodiments 1
The aqueous solution of thing and 50ppm Glus.
Preparation method:
A) the preparation of plant extracts:
S1, aloe extract preparation:Fresh aloe is taken, is cleaned, is dried, squeeze the juice, filters, obtains aloe extract;
S2, cordyceps sinensis flower extract preparation:Fresh cordyceps flower is taken, cleans, dries, adds the water of 5 times of amounts of cordyceps flower weight
Decoct 2 times, each 45min, filtering, take filtrate, produce;
S3, varying strength electric field treatment:Voltage is exposed to after above-mentioned aloe extract and cordyceps sinensis flower extract are mixed
12V, in electric-field intensity 8v/m electric field after 2min, electric-field intensity is adjusted to 5v/m, continues with 32s, is produced.
B) glycerine and stabilizer are mixed, are vortexed after mixing, adds EGF, the plant extract through electric field treatment
Thing, collagen, nonapeptide -1, acetyl group octapeptide -3, Sodium Hyaluronate and water, constantly it is vortexed and forms it into the molten of stable homogeneous
Liquid, sterilize, packaging, produce.
Embodiment 3, a kind of anti-aging skin repair liquid
It is made up of the component of following mass fraction:EGF 6.0 × 10-3%, the plant extract through electric field treatment
Thing 10%, collagen 3.0%, nonapeptide -1 0.01%, acetyl group octapeptide -3 0.01%, stabilizer 1%, glycerine 10%, thoroughly
Bright matter acid sodium 0.05% and water surplus, wherein stabilizer are to contain the Tween-80 degradation product described in 100ppm embodiments 1
With the aqueous solution of 10ppm Glus.
Preparation method reference implementation example 2.
Embodiment 4, a kind of anti-aging skin repair liquid
It is made up of the component of following mass fraction:EGF 1.0 × 10-2%, the plant extract through electric field treatment
It is thing 20%, collagen 5%, nonapeptide -1 0.03%, acetyl group octapeptide -3 0.03%, stabilizer 3%, glycerine 20%, transparent
Matter acid sodium 1.0% and water surplus, wherein stabilizer be containing the Tween-80 degradation product described in 1000ppm embodiments 1 and
The aqueous solution of 100ppm Glus.
Preparation method reference implementation example 2.
Embodiment 5, a kind of anti-aging skin repair liquid
The difference of embodiment 5 and embodiment 2 is, replaces Glu with serine, remaining parameter and operation are strictly according to the facts
Apply shown in example 2.
Embodiment 6, a kind of anti-aging skin repair liquid
The difference of embodiment 6 and embodiment 2 is, replaces Glu with glycine, remaining parameter and operation are strictly according to the facts
Apply shown in example 2.
Comparative example 1, a kind of anti-aging skin repair liquid
Difference with embodiment 2 is, removes step S3, remaining parameter and operation such as embodiment 2.
Comparative example 2, a kind of anti-aging skin repair liquid
Difference with embodiment 2 is that step S3 is exposure after mixing above-mentioned aloe extract and cordyceps sinensis flower extract
2min32s (the removing low strengh electric field processing step) in voltage 12V, electric-field intensity 8v/m electric field.
Test example one, anti-aging experiment
1.1 elasticities, glossiness and wettability evaluation:Crowd on probation is randomly divided into 7 groups, every group of 25 people, tries out crowd
Product composition is not known about, excludes influence of the psychological factor to the product overall evaluation.7 groups of subjects are early later than the phase of cheek daily
Embodiment 2~6 and the reparation liquid prepared by comparative example 1~2 are smeared respectively with region, to product effect after continuous use 30 days
The evaluation criterion of according to the form below 1 is evaluated, and evaluation result is as shown in table 2.
1.2 use feelings are evaluated:By the evaluation criterion of table 3 to using when tingling sensation degree evaluate, evaluation result such as table 4
It is shown.
2. result of the test.
2.1 elasticities, glossiness and wettability evaluation
The evaluation criterion of table 1
Index | 0~3 point | 4~7 points | 8~10 points |
Elasticity | DeGrain | Effect is general | Significant effect |
Glossiness | DeGrain | Effect is general | Significant effect |
Wettability | DeGrain | Effect is general | Significant effect |
The evaluation result of table 2
2.2 use feelings are evaluated
The evaluation criterion of table 3
Index | 0~3 point | 4~7 points | 8~10 points |
Tingling sensation | In the presence of strong tingling sensation | In the presence of slight tingling sensation | Stingless Severe Pain |
The evaluation result of table 4
Group | Fraction |
Embodiment 2 | 9.83 |
Embodiment 3 | 9.61 |
Embodiment 4 | 9.55 |
Embodiment 5 | 9.32 |
Embodiment 6 | 9.28 |
Comparative example 1 | 4.79 |
Comparative example 2 | 6.85 |
2. interpretation of result
2.1 observation of curative effect:As shown in Table 2, there is the skin repair liquid of the embodiment of the present invention 2~6 pole to significantly increase
The effect of skin elasticity degree, glossiness and wettability, there are significant anti-aging effects, wherein with the effect of embodiment 2 most
To be notable.And compared with Example 2, the anti-aging effects scoring of the skin repair liquid of comparative example 1~2 has declined.
2.2 use feelings are evaluated:From table 4, it can be seen that the skin repair liquid-based sheet of the embodiment of the present invention 2~6 is used
Local tingling sensation will not be caused;And it should be noted that compared with Example 2, comparative example 1 (compared with Example 2, is omitted
Electric field treatment step) scoring is 4.79 points, this explanation, after having used skin repair liquid described in comparative example 1, most of consumer
Feel slight stimulation pain be present, represent that electric field treatment advantageously reduces excitant of the plant extracts to skin;Use
After comparative example 2 (compared with Example 2, eliminating low strengh electric field processing step) described skin repair liquid, pain scoring is stimulated
Raised compared with comparative example 2, but from the point of view of scoring, slight stimulation pain when still feeling to use with the presence of some patientss.
Test example two, estimation of stability
2.1 stabilizers form the investigation to activities of epidermal growth factor
The skin repair composition of Example 2~4, and according to the form below 5 sets each control on the basis of embodiment 2
Group, and then each control group anti-aging skin is prepared and repairs liquid, embodiment 2~4 and each control group skin repair group are set
The μ g/L of concentration 8 of initial EGF in compound, each group is placed under conditions of -4 DEG C, 20 DEG C and normal temperature and stored
After 30d, using ELASA methods detect EGF content (kit is purchased from RapidBio companies of the U.S., experimental implementation
Illustrating to carry out by kit) result of the test is as shown in table 6 below.
The different stabilizers of table 5 form
The stabilizer of table 6 forms the influence to Egf Content
Note:With blank control group compared with item,**P < 0.01,*P < 0.05;With 2 groups of embodiment compared with item,##P <
0.01,#P < 0.05.
As shown in Table 6, each group sample stores the content no significant difference of growth factor after 30d at -20 DEG C, without system
Meter learns meaning.
Compared with blank control group (being added without stabilizer), the skin repair liquid of the embodiment of the present invention 2~4 is at 4 DEG C and often
The content of EGF has notable rise after storage 30d under conditions of temperature, has extremely significant difference (P < 0.01).
And surprisingly, compared with 2 groups of embodiment, 1. control group (compared with Example 2, eliminates L- glutamy
Amine) skin repair liquid stores 30d under conditions of 4 DEG C and normal temperature, and Egf Content is decreased significantly (P < 0.05);
2. control group (compared with Example 2, eliminates Tween-80 degradation product) skin repair liquid stores up in the condition of 4 DEG C and normal temperature
After depositing 30d, Egf Content has pole to be remarkably decreased (P < 0.01).This explanation, both, which interact, achieves collaboration increasing
The effect of effect.
Control group 3. (compared with Example 2, stabilizer is Tween-80) skin repair liquid in 4 DEG C and the bar of normal temperature
30d is stored under part, Egf Content is also decreased significantly (P < 0.05) compared with Example 2, this explanation, with addition
Complete Tween-80 is compared, and preferably stable effect can be obtained by adding Tween-80 degradation product collaboration Glu
Fruit.
2.2 stabilizers composition repairs the influence of liquid clarity to each group anti-aging skin
Take above-mentioned 2.1 each group anti-aging skin to repair liquid, be placed on as 25 ± 2 DEG C of temperature, relatively wet 60 ± 10%
Under the conditions of store 3,6,9,12 months after, observe each anti-aging skin and repair liquid clarification situation, and record, result of the test such as table 7
It is shown.
Influence of the stabilizer of table 7 to each group skin repair composition clarity
As shown in Table 7,2~4 anti-aging skin of the present invention repair liquid 25 ± 2 DEG C of temperature, relative humidity 60 ±
Still clarified after being stored 12 months under conditions of 10%, it is with adding the effect of Tween-80 (control group is 3.) acquirement without bright
Significant difference is other, this explanation, and stabilizer of the present invention can obtain equal with Tween-80 in terms of protein mutually aggregation is prevented
Effect.And the anti-aging skin for being added without stabilizer is repaired to have to precipitate a little after liquid stores 6 months under the same conditions and produced,
Supposition is that there occurs the mutual aggregation of small range to cause for collagen and polypeptide.
And it is worth noting that, 2. control group has been simply added into Glu, it is stored 9 months under the same conditions
After occur precipitation;1. control group has been simply added into Tween-80 degradation product, its stablizing effect and embodiment 2 to both
Compare, also with marked difference, there is precipitation in it after storing under the same conditions 9 months, this explanation, polysorbate-
The mutual aggregation of 80 degradation products and Glu to protein has certain inhibitory action, but acts on not notable.And two
Person, which combines, makes this inhibitory action become more significantly, and this explanation, both Glu and Tween-80 degradation product obtain
The effect of Synergistic.
In summary, can be seen that the composition of stabilizer of the present invention or the change of content from this test example can cause skin
The stability of skin remediation composition has large effect, and this influence is not expectable, and inventor thinks to achieve expectation not
The technique effect arrived.
The above-described embodiments merely illustrate the principles and effects of the present invention, not for the limitation present invention.It is any ripe
Know the personage of this technology all can carry out modifications and changes under the spirit and scope without prejudice to the present invention to above-described embodiment.Cause
This, those of ordinary skill in the art is complete without departing from disclosed spirit and institute under technological thought such as
Into all equivalent modifications or change, should by the present invention claim be covered.
Claims (10)
1. a kind of anti-aging skin repairs liquid, it is characterised in that is made up of the component of following mass fraction:EGF
5.0×10-3~1.0 × 10-2%, the plant extracts 10~20% through electric field treatment, collagen 3~6%, nonapeptide -1
0.01~0.05%, acetyl group octapeptide -3 0.01~0.05%, stabilizer 1~3%, glycerine 10~22%, Sodium Hyaluronate
0.05~1.5% and water surplus.
2. liquid is repaired as claimed in claim 1, it is characterised in that be made up of the component of following mass fraction:Epidermal growth factor
Son 6.0 × 10-3~1.0 × 10-2%, the plant extracts 15~20% through electric field treatment, collagen 3~5%, nonapeptide -1
0.01~0.03%, acetyl group octapeptide -3 0.01~0.03%, stabilizer 1~3%, glycerine 10~20%, Sodium Hyaluronate
0.05~1.0% and water surplus.
3. liquid is repaired as claimed in claim 2, it is characterised in that be made up of the component of following mass fraction:Epidermal growth factor
Son 8.0 × 10-3%, the plant extracts 15% through electric field treatment, collagen 3.5%, nonapeptide -1 0.02%, acetyl group eight
Peptide -3 0.02%, stabilizer 1.5%, glycerine 15%, Sodium Hyaluronate 0.05% and water surplus.
4. the reparation liquid as described in claims 1 to 3 is any, it is characterised in that the plant extracts through electric field treatment by
Following steps are made:
S1, aloe extract preparation:Fresh aloe is taken, is cleaned, is dried, squeeze the juice, filters, obtains aloe extract;
S2, cordyceps sinensis flower extract preparation:Fresh cordyceps flower is taken, cleans, dries, adds the decocting of 3~6 times of amounts of cordyceps flower weight
Boil 1~3 time, 30~60min, filtering, takes filtrate, produce every time;
S3, varying strength electric field treatment:Will above-mentioned aloe extract and cordyceps sinensis flower extract mix after exposed to voltage 10~
23V, in 8~10v/m of electric-field intensity electric field after 1~3min, electric-field intensity is adjusted to 3~8v/m, continue with 20~
45s, produce.
5. liquid is repaired as claimed in claim 4, it is characterised in that extract aloe extract and cordyceps flower in the step S3
10~20V is exposed to after thing mixing, in 8~10v/m of electric-field intensity electric field after 1~3min, electric-field intensity is adjusted to 5~
8v/m, handle 20~50s.
6. the reparation liquid as described in claims 1 to 3 is any, it is characterised in that the stabilizer is to include 100~1000ppm
The aqueous solution of Tween-80 degradation product and 10~100ppm hydrophilic amino acids.
7. liquid is repaired as claimed in claim 6, it is characterised in that the Tween-80 degradation product is made by following steps:
Tween-80 solution, the green that 2~5wt% of inoculation concentration is 800~1000cfu/mL are added in toward 100ml shaking flasks
Trichoderma, it is 30~35 DEG C, rotating speed 120r/min to set temperature, cultivates 15d, centrifugation, takes supernatant;Supernatant is carried out successively micro-
Filter, cation exchange and sterilizing, Tween-80 degradation product is made.
8. liquid is repaired as claimed in claim 6, it is characterised in that the hydrophilic amino acid is selected from Glu, serine
With one kind in glycine.
9. liquid is repaired as claimed in claim 8, it is characterised in that the hydrophilic amino acid is selected from Glu.
A kind of 10. method for preparing the skin repair liquid as described in claim 1~9 is any, it is characterised in that including following step
Suddenly:
Glycerine and stabilizer are mixed, are vortexed after mixing, adds EGF, the plant extracts through electric field treatment, glue
Former albumen, nonapeptide -1, acetyl group octapeptide -3, Sodium Hyaluronate and water, constantly it is vortexed and forms it into the solution of stable homogeneous, go out
Bacterium, packaging, produces.
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Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
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CN115462524A (en) * | 2022-08-29 | 2022-12-13 | 杭州佰倍优生物科技有限公司 | A blood peptide with skin caring and antiaging effects, and its preparation method |
-
2017
- 2017-11-30 CN CN201711243475.3A patent/CN107875093A/en not_active Withdrawn
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
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CN115462524A (en) * | 2022-08-29 | 2022-12-13 | 杭州佰倍优生物科技有限公司 | A blood peptide with skin caring and antiaging effects, and its preparation method |
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