CN107874250A - A kind of preparation method of tara vine ferment - Google Patents
A kind of preparation method of tara vine ferment Download PDFInfo
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Abstract
本发明公开了一种软枣猕猴桃酵素的制作方法,软枣猕猴桃鲜果不耐储藏,常温条件下极易变软,采用梯度冻结法进行处理,不仅提高了贮藏性,同时也极大程度地保留了软枣猕猴桃鲜果中的营养成分和生物活性物质,同时梯度解冻方式能够缩短解冻时间,适合工业化生产。采用两次预煮法,提高了果汁的出汁率,同时二次预煮能够使果实中的营养成分较大程度的溶出,提高了果实的利用率。打浆过程中加入适量VC,抑制了果汁的褐变,保证了软枣猕猴桃果实的真实颜色,色泽均一,具有清新的果香。产品在保留果实原有营养成分之外,经过发酵,增加了超氧化物歧化酶(SOD)、脂肪酶和蛋白酶等有益酶类,增加了保健功效。The invention discloses a method for preparing kiwi fruit enzymes. The fresh kiwi fruit of soft dates is not resistant to storage and is easily softened under normal temperature conditions. The gradient freezing method is used for processing, which not only improves the storability, but also greatly retains the fruit. The nutritional components and bioactive substances in the fresh kiwi fruit of Jujube were extracted, and the gradient thawing method can shorten the thawing time, which is suitable for industrial production. The twice precooking method improves the juice yield of the fruit juice, and at the same time, the second precooking can dissolve the nutrients in the fruit to a greater extent, thereby improving the utilization rate of the fruit. A proper amount of V C is added during the beating process, which inhibits the browning of the fruit juice and ensures the true color of the kiwi fruit, which is uniform in color and has a fresh fruity aroma. In addition to retaining the original nutritional content of the fruit, the product has been fermented to increase beneficial enzymes such as superoxide dismutase (SOD), lipase and protease, increasing the health care effect.
Description
技术领域technical field
本发明涉及食品加工技术领域,尤其涉及一种软枣猕猴桃酵素的制作方法。The invention relates to the technical field of food processing, in particular to a method for preparing kiwi fruit ferment.
背景技术Background technique
软枣猕猴桃(Actinide arguta planch)别名软枣子、藤枣,是猕猴桃属、猕猴桃科大型落叶藤本植物,在我国境内分布广泛,从东北地区直到广西均有分布。猕猴桃含有丰富的氨基酸和维生素,其中氨基酸的种类可以达到二十余种,而维生素中VC的含量尤其突出,甚至可以达到其他水果中VC含量的几十倍。软枣猕猴桃具有防癌、清热解毒、利湿、补虚益损、保护心脑血管等药用功效,是一种食用价值和药用价值较高的食品,非常适合开发生产功能保健食品,在实际应用中,其产品主要有果酱、蜜饯、罐头等。Actinide arguta planch (Actinide arguta planch), also known as soft jujube and vine jujube, is a large deciduous vine of the genus Actinide and Actinaceae. It is widely distributed in my country, from Northeast China to Guangxi. Kiwi fruit is rich in amino acids and vitamins, among which there are more than 20 types of amino acids, and the content of vitamin C is particularly prominent, even reaching dozens of times the content of vitamin C in other fruits. Jujube kiwifruit has medicinal effects such as preventing cancer, clearing away heat and detoxification, promoting dampness, tonifying deficiency and benefiting loss, and protecting cardiovascular and cerebrovascular. It is a food with high edible and medicinal value, and is very suitable for the development and production of functional health food. In practical application, its products mainly include jam, candied fruit, canned food and so on.
酵素又称为酶(enzyme),以一种富含有益酶类的生物活性大分子,是近年来从日本和我国台湾等地流行起来的功能食品。按其形态分类可以分为固体酵素和液体酵素。本产品是以软枣猕猴桃鲜果为原料,通过有益菌发酵制成的富含VC、氨基酸、脂肪酶、蛋白酶和超氧化物歧化酶(SOD)等有益成分的一种液体酵素产品。具有抗癌、清热护肝、保护心脑血管,调节肠道微生物等多种保健功能。Enzyme, also known as enzyme (enzyme), is a biologically active macromolecule rich in beneficial enzymes. It is a functional food that has become popular in Japan and Taiwan in recent years. According to its form classification, it can be divided into solid enzyme and liquid enzyme. This product is a liquid enzyme product rich in vitamin C , amino acid, lipase, protease and superoxide dismutase (SOD) and other beneficial ingredients, which are made from the fresh fruit of kiwifruit. It has various health functions such as anti-cancer, clearing heat and protecting liver, protecting cardiovascular and cerebrovascular, regulating intestinal microorganisms, etc.
目前,市场上关于软枣猕猴桃的功能性饮品尚未发现,如果将软枣猕猴桃制成液体酵素,由于液体物质容易吸收,能够更大程度的发挥软枣猕猴桃酵素中各种有益成分的作用。同时,随着近些年来酵素产品的流行,我国自主生产的酵素产品还不多见,因此酵素产品具有良好的发展前景。本发明通过复合菌种发酵制取的软枣猕猴桃酵素可作为市场中新型的食品,具有较多的生物活性功能,并且颜色均一,具有清新的果香和发酵产品特有的香气,将会具有广阔的前景和消费空间。At present, there is no functional drink of kiwi fruit on the market. If the kiwi fruit is made into a liquid enzyme, since the liquid substance is easy to absorb, it can play the role of various beneficial ingredients in the kiwi fruit enzyme to a greater extent. At the same time, with the popularity of enzyme products in recent years, there are not many enzyme products produced independently in my country, so enzyme products have good development prospects. The present invention can be used as a new type of food in the market by fermenting the fermented fermented kiwi fruit of the present invention. It has more biologically active functions, and is uniform in color. prospect and consumption space.
发明内容Contents of the invention
基于背景技术存在的技术问题,本发明提出了一种软枣猕猴桃酵素的制作方法。Based on the technical problems existing in the background technology, the present invention proposes a preparation method of Actinidia jujube enzyme.
本发明的技术方案如下:Technical scheme of the present invention is as follows:
一种软枣猕猴桃酵素的制作方法,包括以下步骤:A preparation method of kiwi fruit ferment, comprising the following steps:
A、梯度冻结:将采后的软枣猕猴桃鲜果进行速冻后在低温下保存;解冻时,采用水—低频解冻复合解冻工艺,通过水解冻,待软枣猕猴桃表面温度升高至-10℃~-8℃左右时,再采用低频解冻的方式进行解冻;A. Gradient freezing: Quick-freeze the fresh kiwi fruit after harvesting and store it at low temperature; when thawing, use the combined thawing process of water-low frequency thawing, and thaw it with water until the surface temperature of the kiwi fruit surface rises to -10℃~ When the temperature is around -8°C, thaw by low-frequency thawing;
B、清洗:选择成熟度一致、软硬适度、无病虫害、果实饱满的软枣猕猴桃鲜果,去除果柄和果蒂,洗净;B. Cleaning: Select the fresh kiwi fruit with the same maturity, moderate hardness, no pests and diseases, and full fruit, remove the stalk and pedicle, and wash;
C、果汁制取:清洗后加入适量的热水及适量VC,待果实变软后,使用打浆机进行匀浆,用纱布过滤掉果渣及果籽,并加入适量水再次煮沸,使果实中更多的营养成分溶出,提高果实利用率,滤掉果渣;C. Fruit juice preparation: After cleaning, add an appropriate amount of hot water and an appropriate amount of V C. After the fruit becomes soft, use a beater to homogenate, filter out the pomace and fruit seeds with gauze, and add an appropriate amount of water to boil again to make the fruit More nutrients are dissolved in the fruit, which improves the utilization rate of the fruit and filters out the pomace;
D、酵母菌发酵:按照重量比向果汁中加入白砂糖和酿酒酵母粉,在28℃~30℃条件下,充分溶氧,发酵8h~10h;D. Yeast fermentation: add white sugar and Saccharomyces cerevisiae powder to the fruit juice according to the weight ratio, fully dissolve oxygen at 28°C-30°C, and ferment for 8h-10h;
E、嗜酸乳杆菌发酵:再加入嗜酸乳杆菌菌粉,37℃下发酵24h~30h,再置于4℃条件下10h~12h进行后发酵;E. Lactobacillus acidophilus fermentation: add Lactobacillus acidophilus bacteria powder, ferment at 37°C for 24h-30h, then put it at 4°C for 10h-12h for post-fermentation;
F、调配:将得到的软枣猕猴桃发酵液加入VC和果葡糖浆进行调配;F, allotment: the kiwifruit fermented liquid of jujube that obtains is added V C and high fructose syrup to allot;
G、灭菌:将调配后的软枣猕猴桃酵素灭菌,迅速降温至4℃~5℃。G. Sterilization: Sterilize the fermented kiwifruit ferment after deployment, and quickly cool down to 4°C-5°C.
H、灌装:将灭菌后的酵素灌入玻璃瓶中,密封,即为成品。H. Filling: Pour the sterilized enzyme into a glass bottle and seal it, which is the finished product.
优选的,所述的步骤A中,梯度冻结的主要工艺参数为:速冻温度为-25℃~-30℃,冻结时间为15~25min,低温保存温度为-18℃~-20℃。Preferably, in the step A, the main process parameters of gradient freezing are: quick freezing temperature is -25°C to -30°C, freezing time is 15 to 25min, and low temperature storage temperature is -18°C to -20°C.
优选的,所述的步骤A中,水—低频解冻复合解冻工艺,包括以下步骤:先通过水解冻,待软枣猕猴桃表面温度升高至-10℃~-8℃左右时,再采用低频解冻的方式进行解冻。Preferably, in the step A, the water-low-frequency thawing composite thawing process includes the following steps: first thawing with water, and then using low-frequency thawing when the surface temperature of the kiwi fruit surface rises to about -10°C ~ -8°C to defrost.
优选的,所述的步骤C中,VC的加入量为0.2%~0.5%,煮沸时间为45~90s。Preferably, in the step C, the amount of VC added is 0.2%-0.5%, and the boiling time is 45-90s.
优选的,所述的步骤D中,按照重量比向果汁中加入7%~9%的白砂糖和0.2~0.8%的酿酒酵母粉。Preferably, in the step D, 7%-9% white granulated sugar and 0.2-0.8% brewer's yeast powder are added to the fruit juice according to the weight ratio.
优选的,所述的步骤E中,按照2~3%的接种量加入嗜酸乳杆菌菌粉。Preferably, in the step E, the Lactobacillus acidophilus powder is added according to the inoculum amount of 2-3%.
优选的,所述的步骤F中,具体工艺参数:VC的加入量为0.2%~1.2%,果葡糖浆的加入量为1%~3%。Preferably, in the step F, specific process parameters: the added amount of V C is 0.2%-1.2%, and the added amount of fructose syrup is 1%-3%.
优选的,所述的步骤G中,灭菌条件为:75~85℃下灭菌25~40min。Preferably, in the step G, the sterilization condition is: sterilization at 75-85°C for 25-40 minutes.
本发明的优点在于:1.软枣猕猴桃鲜果不耐储藏,常温条件下极易变软,采用梯度冻结法进行处理,不仅提高了贮藏性,同时也极大程度地保留了软枣猕猴桃鲜果中的营养成分和生物活性物质,同时梯度解冻方式能够缩短解冻时间,适合工业化生产。2.采用两次预煮法,首先在打浆之前进行预煮,匀浆、过滤后对果渣再次预煮处理,提高了果汁的出汁率,同时二次预煮能够使果实中的营养成分较大程度的溶出,提高了果实的利用率。3.打浆过程中加入适量VC,抑制了果汁的褐变,所制得的软枣猕猴桃酵素颜色呈黄绿色,基本保证了软枣猕猴桃果实的真实颜色,色泽均一,具有清新的果香。4.产品在保留果实原有营养成分之外,经过发酵,增加了超氧化物歧化酶(SOD)、脂肪酶和蛋白酶等有益酶类,增加了保健功效。The advantages of the present invention are: 1. The fresh fruit of Actinidia jujube is not resistant to storage, and it is very easy to become soft under normal temperature conditions. The gradient freezing method is used for processing, which not only improves the storage performance, but also greatly retains the fresh fruit of Actinidia jujube. Nutrients and biologically active substances, and the gradient thawing method can shorten the thawing time, which is suitable for industrial production. 2. Adopt twice precooking method, first precook before beating, homogenate, filter and precook the pomace again, improve the juice yield of fruit juice, and at the same time, the second precooking can make the nutrients in the fruit less The maximum degree of dissolution improves the utilization rate of the fruit. 3. A proper amount of V C is added during the beating process to inhibit the browning of the fruit juice, and the obtained kiwi fruit enzyme color is yellow-green, which basically ensures the true color of the kiwi fruit fruit, with uniform color and fresh fruity aroma. 4. In addition to retaining the original nutrients of the fruit, the product has been fermented to increase beneficial enzymes such as superoxide dismutase (SOD), lipase and protease, increasing the health care effect.
具体实施方式Detailed ways
实施例1Example 1
一种软枣猕猴桃酵素的制作方法,包括以下步骤:A preparation method of kiwi fruit ferment, comprising the following steps:
将速冻的成熟度一致,软硬适度,无病虫害的软枣猕猴桃进行梯度解冻。去除果柄和果蒂,洗净后,加入热水预煮1min,按成品酵素重量计,称取0.3%的VC加入打浆机中进行打浆,经纱布过滤后,将果渣放入热水中继续煮沸1min,再次过滤。按照重量比向软枣猕猴桃果汁中加入7%的白砂糖和0.5%的酿酒酵母粉,28℃条件下发酵8h,再按照2.5%的接种量加入嗜酸乳杆菌菌粉,37℃条件下发酵24h,置于4℃条件下10h进行后发酵。发酵结束后,添加VC和果葡糖浆进行调配,各项配比如下:VC为0.2%,使得加入的VC的总量为0.5%,果葡糖浆为1%。调配后再80℃下灭菌30min,迅速降温至4℃~5℃,灌装即得成品。Gradient thawing is performed on quick-frozen jujube kiwifruit with consistent maturity, moderate hardness, and no pests and diseases. Remove the fruit stalk and fruit pedicle, after washing, add hot water to pre-boil for 1min, weigh 0.3% V C and add it to the beater for beating according to the weight of the finished enzyme, after filtering through gauze, put the pomace into hot water Continue to boil for 1 min in medium, and filter again. According to the weight ratio, add 7% white sugar and 0.5% Saccharomyces cerevisiae powder to the Jujube kiwi fruit juice, ferment at 28°C for 8 hours, then add Lactobacillus acidophilus powder according to the inoculation amount of 2.5%, and ferment at 37°C After 24 hours, post-fermentation was carried out at 4°C for 10 hours. After the fermentation is finished, VC and fructose syrup are added for deployment, and the ratios are as follows: VC is 0.2%, so that the total amount of added VC is 0.5%, and fructose syrup is 1%. After blending, sterilize at 80°C for 30 minutes, quickly cool down to 4°C-5°C, and fill to obtain the finished product.
实施例2Example 2
一种软枣猕猴桃酵素的制作方法,包括以下步骤:A preparation method of kiwi fruit ferment, comprising the following steps:
将速冻的成熟度一致,软硬适度,无病虫害的软枣猕猴桃进行梯度解冻。去除果柄和果蒂,洗净后,加入热水预煮1min,按成品酵素重量计,称取0.5%的VC加入打浆机中进行打浆,经纱布过滤后,将果渣放入热水中继续煮沸1min,再次过滤。按照重量比向软枣猕猴桃果汁中加入8%的白砂糖和0.2%的酿酒酵母粉,充分溶氧,28℃条件下发酵9h,再按照2%的接种量加入嗜酸乳杆菌菌粉,37℃条件下发酵27h,置于4℃条件下11h进行后发酵。发酵结束后,添加VC和果葡糖浆进行调配,各项配比如下:VC为0.7%,使得加入的VC的总量为1.0%,果葡糖浆为2%。调配后再80℃下灭菌30min,迅速降温至4℃~5℃,灌装即得成品。Gradient thawing is performed on quick-frozen jujube kiwifruit with consistent maturity, moderate hardness, and no pests and diseases. Remove the fruit stalk and fruit pedicle, after washing, add hot water to pre-boil for 1min, weigh 0.5% V C into the beater for beating according to the weight of the finished enzyme, filter through gauze, put the pomace into hot water Continue to boil for 1 min in medium, and filter again. According to the weight ratio, add 8% white granulated sugar and 0.2% Saccharomyces cerevisiae powder to the jujube kiwi fruit juice, fully dissolve oxygen, ferment at 28°C for 9 hours, then add Lactobacillus acidophilus powder according to the inoculation amount of 2%, 37 Under the condition of ℃, ferment for 27h, and put it under the condition of 4℃ for 11h for post-fermentation. After the fermentation is finished, VC and fructose syrup are added for deployment, and the ratios are as follows: VC is 0.7%, so that the total amount of added VC is 1.0%, and fructose syrup is 2%. After blending, sterilize at 80°C for 30 minutes, quickly cool down to 4°C-5°C, and fill to obtain the finished product.
实施例3Example 3
一种软枣猕猴桃酵素的制作方法,包括以下步骤:A preparation method of kiwi fruit ferment, comprising the following steps:
将速冻的成熟度一致,软硬适度,无病虫害的软枣猕猴桃进行梯度解冻。去除果柄和果蒂,洗净后,加入热水预煮1min,按成品酵素重量计,称取0.2%的VC加入打浆机中进行打浆,经纱布过滤后,将果渣放入热水中继续煮沸1min,再次过滤。按照重量比向软枣猕猴桃果汁中加入9%的白砂糖和0.8%的酿酒酵母粉,充分溶氧,28℃条件下发酵10h,再按照3%的接种量加入嗜酸乳杆菌菌粉,37℃条件下发酵30h,置于4℃条件下12h进行后发酵。发酵结束后,添加VC、白砂糖和果葡糖浆进行调配,各项配比如下:VC为1.2%,使得加入的VC的总量为1.5%,果葡糖浆为3%。调配后再80℃下灭菌30min,迅速降温至4℃~5℃,灌装即得成品。Gradient thawing is performed on quick-frozen jujube kiwifruit with consistent maturity, moderate hardness, and no pests and diseases. Remove fruit stalks and fruit pedicles, wash them, add hot water to pre-boil for 1min, weigh 0.2% V C into a beater for beating based on the weight of the finished enzyme, filter through gauze, and put the pomace into hot water Continue to boil for 1 min in medium, and filter again. According to the weight ratio, add 9% white granulated sugar and 0.8% Saccharomyces cerevisiae powder to the jujube kiwi fruit juice, fully dissolve oxygen, ferment at 28°C for 10h, then add Lactobacillus acidophilus powder according to the inoculation amount of 3%, 37 Under the condition of ℃, ferment for 30h, and put it under the condition of 4℃ for 12h for post-fermentation. After the fermentation is finished, add VC , white granulated sugar and fructose syrup for deployment. The proportions are as follows: VC is 1.2%, so that the total amount of added VC is 1.5%, and fructose syrup is 3%. After blending, sterilize at 80°C for 30 minutes, quickly cool down to 4°C-5°C, and fill to obtain the finished product.
对比例1Comparative example 1
将实施例1中梯度冻结工序改成直接冷冻,其余制作方法不变。The gradient freezing process in Example 1 is changed to direct freezing, and the rest of the production methods remain unchanged.
对比例2Comparative example 2
将实施例1中二次预煮工序去除,其余制作方法不变。The secondary precooking process in Example 1 is removed, and all the other preparation methods are unchanged.
对比例3Comparative example 3
将实施例1中的VC去除,其余制作方法不变。The VC in Example 1 is removed, and the rest of the production methods remain unchanged.
以下对实施例1-3和对比例1-3制作的软枣猕猴桃酵素进行检测,得到如下检测数据,具体数据见表1;The Actinidia jujube ferment that embodiment 1-3 and comparative example 1-3 are made detects below, obtains following detection data, and concrete data is shown in Table 1;
表1:实施例1-3和对比例1-3制作的软枣猕猴桃酵素的检测数据Table 1: The detection data of the Actinidia jujube enzyme that embodiment 1-3 and comparative example 1-3 make
由以上测试数据可以知道,梯度冻结工序、二次预煮工序、以及加入VC均对产品的品质有明显的影响。From the above test data, it can be known that the gradient freezing process, the second precooking process, and the addition of VC all have a significant impact on the quality of the product.
以上所述,仅为本发明较佳的具体实施方式,但本发明的保护范围并不局限于此,任何熟悉本技术领域的技术人员在本发明揭露的技术范围内,根据本发明的技术方案及其发明构思加以等同替换或改变,都应涵盖在本发明的保护范围之内。The above is only a preferred embodiment of the present invention, but the scope of protection of the present invention is not limited thereto, any person familiar with the technical field within the technical scope disclosed in the present invention, according to the technical solution of the present invention Any equivalent replacement or change of the inventive concepts thereof shall fall within the protection scope of the present invention.
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CN109480140A (en) * | 2018-12-24 | 2019-03-19 | 湖南优蜜食品科技有限公司 | A kind of orientation regulation fermentation Kiwi berry fruit juice and pulp partical beverage and preparation method thereof |
CN112618427A (en) * | 2019-10-07 | 2021-04-09 | 百岳特生物科技(上海)有限公司 | Kiwi fruit fermented juice and application thereof for improving skin condition |
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CN115530365A (en) * | 2022-10-28 | 2022-12-30 | 广西民族师范学院 | A kind of chicken skin fruit - pineapple - dragon fruit compound enzyme and preparation method thereof |
CN115530365B (en) * | 2022-10-28 | 2024-04-09 | 广西民族师范学院 | Pitaya-pineapple-dragon fruit compound enzyme and preparation method thereof |
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