CN107861360A - Based on single exposure of multi-angle illumination multiplexing without lens imaging system and method - Google Patents
Based on single exposure of multi-angle illumination multiplexing without lens imaging system and method Download PDFInfo
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- CN107861360A CN107861360A CN201711385321.8A CN201711385321A CN107861360A CN 107861360 A CN107861360 A CN 107861360A CN 201711385321 A CN201711385321 A CN 201711385321A CN 107861360 A CN107861360 A CN 107861360A
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- 238000005286 illumination Methods 0.000 title claims abstract description 100
- 238000003384 imaging method Methods 0.000 title claims abstract description 76
- 238000000034 method Methods 0.000 title claims abstract description 14
- 238000005516 engineering process Methods 0.000 claims abstract description 6
- VYZAMTAEIAYCRO-UHFFFAOYSA-N Chromium Chemical compound [Cr] VYZAMTAEIAYCRO-UHFFFAOYSA-N 0.000 claims abstract description 5
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- 239000011651 chromium Substances 0.000 claims abstract description 5
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- 230000001427 coherent effect Effects 0.000 claims description 10
- 239000011159 matrix material Substances 0.000 claims description 8
- 230000003287 optical effect Effects 0.000 claims description 6
- 230000003068 static effect Effects 0.000 claims description 5
- 230000000747 cardiac effect Effects 0.000 claims description 4
- 238000006073 displacement reaction Methods 0.000 claims description 4
- 230000003595 spectral effect Effects 0.000 claims description 4
- 230000000007 visual effect Effects 0.000 description 3
- 230000000694 effects Effects 0.000 description 2
- 239000000463 material Substances 0.000 description 2
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- 238000010586 diagram Methods 0.000 description 1
- 238000004043 dyeing Methods 0.000 description 1
- 238000011156 evaluation Methods 0.000 description 1
- 239000000835 fiber Substances 0.000 description 1
- 238000005194 fractionation Methods 0.000 description 1
- 230000004927 fusion Effects 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 230000003993 interaction Effects 0.000 description 1
- 238000005259 measurement Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000005457 optimization Methods 0.000 description 1
- 238000012360 testing method Methods 0.000 description 1
- 230000004304 visual acuity Effects 0.000 description 1
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- G—PHYSICS
- G03—PHOTOGRAPHY; CINEMATOGRAPHY; ANALOGOUS TECHNIQUES USING WAVES OTHER THAN OPTICAL WAVES; ELECTROGRAPHY; HOLOGRAPHY
- G03H—HOLOGRAPHIC PROCESSES OR APPARATUS
- G03H1/00—Holographic processes or apparatus using light, infrared or ultraviolet waves for obtaining holograms or for obtaining an image from them; Details peculiar thereto
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- G—PHYSICS
- G03—PHOTOGRAPHY; CINEMATOGRAPHY; ANALOGOUS TECHNIQUES USING WAVES OTHER THAN OPTICAL WAVES; ELECTROGRAPHY; HOLOGRAPHY
- G03H—HOLOGRAPHIC PROCESSES OR APPARATUS
- G03H1/00—Holographic processes or apparatus using light, infrared or ultraviolet waves for obtaining holograms or for obtaining an image from them; Details peculiar thereto
- G03H1/04—Processes or apparatus for producing holograms
- G03H1/0402—Recording geometries or arrangements
- G03H2001/0436—Holographic camera
Abstract
Exposed the invention discloses a kind of based on the single of multi-angle illumination multiplexing without lens imaging system and method, wherein, system includes：Accurate monochromatic and PLC technology LED panel, for producing multi-angle illumination, and is radiated on the circular hole placed below simultaneously；Chromium plating is used for blocking illumination and with the circular hole mask of energy printing opacity circular hole on a glass, for coordinating multi-angle illumination；Camera detector is used to gather the sample diffraction pattern under single exposure, and camera detector distance sample pre-determined distance, to reduce the aliasing of effective information under different illumination；Controller, the illumination pattern in Synchronization Control LED panel gather with camera, to be imaged to the sample being arranged between circular hole mask and camera detector, obtain reconstruction image.The system can effectively improve the acquisition speed of no lens imaging system, realize single exposure without lens imaging, dynamic sample can be observed, it is simple easily to realize.
Description
Technical field
The present invention relates to without lens imaging technical field, more particularly to a kind of single exposure nothing based on multi-angle illumination multiplexing
Lens imaging system and method.
Background technology
It is a kind of not need imaging len and gather diffraction pattern to recover the imaging skill of sample complex amplitude without lens imaging
Art, there is to observe field range big, simple system, compact and cheap.Camera sensor has towards more pixel quantities
The trend that smaller pixel unit direction is developed；Meanwhile the ability more and more higher of the numerical computations of computer so that without camera lens
Become a kind of selection of micro-imaging, used in many applications.There is lens optical system compared to traditional,
The diffraction pattern of object is directly gathered using an optical-electrical converter without lens imaging system, and reconstructs complex amplitude information.Figure
Phase Retrieve Algorithm is used as rebuilding, phase imaging can be realized to transparent sample.In addition, without lens imaging can solve microscope into
The contradiction of visual field and resolution ratio as in, both are decoupled, realizes the data acquisition of high spatial bandwidth product.
Under normal circumstances, no lens imaging, using LASER Light Source either LED couple single-mode fiber provide it is relevant or
Partially coherent illumination.Illumination is traveled on sample through an aperture, and directly gathers diffraction pattern with a camera sensor.
Traditional the methods of needing to scan using aperture mechanical scanning, the collection of more distances or multi-angle illumination without lens imaging, collection
Multiple diffraction patterns carry out the fusion of image information, to obtain preferable image reconstruction quality and accurate phase information.Cause
This, it is very slow as a kind of imaging technique for needing repeatedly to measure, the picking rate of no lens imaging, it is impossible to be used in dynamic sample
Observation.This is one of major defect of no lens imaging, and one has the problem of to be solved.
The content of the invention
It is contemplated that at least solves one of technical problem in correlation technique to a certain extent.
Therefore, it is an object of the present invention to propose that a kind of list based on multi-angle illumination multiplexing is exposed without lens imaging
System, the system effectively improve the acquisition speed of no lens imaging system, realize single exposure without lens imaging, make it
Dynamic sample can be observed, it is simple easily to realize.
It is another object of the present invention to propose a kind of single exposure being multiplexed based on multi-angle illumination without lens imaging side
Method.
To reach above-mentioned purpose, one aspect of the present invention embodiment proposes a kind of single exposure based on multi-angle illumination multiplexing
Without lens imaging system, including：Accurate monochromatic and PLC technology LED panel, for producing multi-angle illumination, and irradiates simultaneously
On the circular hole placed below；Chromium plating is used for blocking illumination and with the circular hole mask of energy printing opacity circular hole on a glass, is used for
Coordinate the multi-angle illumination, wherein, after light propagates certain distance through the circular hole, there is overlapping region on sample face
Scanning；Camera detector, for gathering the sample diffraction pattern under single exposure, and the camera detector distance sample
Pre-determined distance, to reduce the aliasing of effective information under different illumination；Controller, for the light described in Synchronization Control in LED panel
Gather according to pattern and camera, to be imaged to the sample being arranged between the circular hole mask and the camera detector, obtain
Take reconstruction image.
The embodiment of the present invention based on single exposure of multi-angle illumination multiplexing without lens imaging system, easily build and
Demarcation and calibration that need not be complicated, the quick no lens imaging of individual collection can be realized, so as to effectively improve no camera lens into
As the acquisition speed of system, realize single exposure without lens imaging, dynamic sample can be observed, it is simple easily real
It is existing.
In addition, according to the above embodiment of the present invention exposed without lens imaging system also based on the single of multi-angle illumination multiplexing
There can be following additional technical characteristic：
Further, in one embodiment of the invention, selected section coherent light illumination and unit multiplication factor into
As system, there are the LED that 32*32 small cups are uniformly arranged, the wherein a length of 518nm of cardiac wave, spectral bandwidth 20nm in the LED panel,
And the interval of adjacent LED lamp is 4mm.
Further, in one embodiment of the invention, while 7*7 small cup LEDs are lighted, to provide multiplexed optical photograph, and
Each angular light is according to the directional light for a branch of partially coherent；A LED is lighted per adjacent 4 positions；Obtain more than 1.2*
1.2mm2Field range.
Further, in one embodiment of the invention, it is every on the sample face under the multi-angle illumination
One scanning pattern is all the fresnel diffraction pattern that circular hole propagates preset distance, and under the illumination of different angle, it is described to spread out
Penetrate fresnel diffraction pattern and produce different space displacements.
Further, in one embodiment of the invention, on the circular hole mask size of the circular hole according in institute
Mutual coverage information and the aliasing information stated on sample is made.
Further, in one embodiment of the invention, the pre-determined distance is determined according to the size of the circular hole, with
Scanning on the sample face will meet the mutual overlay area of preparatory condition.
Further, in one embodiment of the invention, the camera detector is monochromatic CMOS cameras, and pixel count is
2040*2448, single pixel size are 3.45um, most fast 35 frames of frame per second/second.
Further, in one embodiment of the invention, in the diffraction pattern, by simple locus not
Together to split out to obtain the effective information under the illumination of the different angle, and the reconstruction image is improved by optimized algorithm
Quality.
To reach above-mentioned purpose, another aspect of the present invention embodiment proposes a kind of single exposure based on multi-angle illumination multiplexing
Light is without lens imaging method, using above-mentioned imaging system, wherein, it the described method comprises the following steps：Gather under no specimen
Diffraction pattern is used to demarcate, while collection has the pattern of sample as the initial data rebuild；By diffraction pattern plus default
Effective information region corresponding to threshold calibration difference illumination, and obtain to the strength values for the different illumination calibrated；According to mark
The pattern for having sample is carried out resolution by effective information region corresponding to the fixed different illumination, to obtain multiple bands
There is the image of aliasing information；The estimate for observation sample information is obtained according to multiple described images with aliasing information,
To make the initial value of optimized algorithm, to solve effective information according to the initial value of the optimized algorithm is counter by optimized algorithm, with
On the basis of the reconstruction image of one side, while the weight matrix under different illumination shared by information is calculated, continuouslyd optimize described
Weight matrix；There is the diffraction image of sample using individual, bring the initial value into, and rebuild with optimized algorithm, described in acquisition
The complex amplitude information of sample, obtain reconstruction image.
The embodiment of the present invention based on single exposure of multi-angle illumination multiplexing without lens imaging method, easily build and
Demarcation and calibration that need not be complicated, the quick no lens imaging of individual collection can be realized, so as to effectively improve no camera lens into
As the acquisition speed of system, realize single exposure without lens imaging, dynamic sample can be observed, it is simple easily real
It is existing.
In addition, according to the above embodiment of the present invention exposed without lens imaging method also based on the single of multi-angle illumination multiplexing
There can be following additional technical characteristic：
Further, in one embodiment of the invention, the pattern for having sample includes a static sample pattern
Or multiple dynamic sample patterns.
The additional aspect of the present invention and advantage will be set forth in part in the description, and will partly become from the following description
Obtain substantially, or recognized by the practice of the present invention.
Brief description of the drawings
Of the invention above-mentioned and/or additional aspect and advantage will become from the following description of the accompanying drawings of embodiments
Substantially and it is readily appreciated that, wherein：
Fig. 1 is the single exposure being multiplexed based on multi-angle illumination according to one embodiment of the invention without lens imaging system
Structural representation；
Fig. 2 is the single exposure being multiplexed based on multi-angle illumination according to one embodiment of the invention without lens imaging system
Structural representation；
Fig. 3 is the single exposure being multiplexed based on multi-angle illumination according to one embodiment of the invention without lens imaging method
Flow chart.
Embodiment
Embodiments of the invention are described below in detail, the example of the embodiment is shown in the drawings, wherein from beginning to end
Same or similar label represents same or similar element or the element with same or like function.Below with reference to attached
The embodiment of figure description is exemplary, it is intended to for explaining the present invention, and is not considered as limiting the invention.
Describe to propose according to embodiments of the present invention with reference to the accompanying drawings is exposed without mirror based on the single of multi-angle illumination multiplexing
Head imaging system and method, describe to propose according to embodiments of the present invention first with reference to the accompanying drawings based on multi-angle illumination multiplexing
Single exposure is without lens imaging system.
Fig. 1 is single structure of the exposure without lens imaging system based on multi-angle illumination multiplexing of one embodiment of the invention
Schematic diagram.
As shown in figure 1, it should be included based on single exposure of multi-angle illumination multiplexing without lens imaging system 10：LED panel
100th, circular hole mask 200, camera detector 300 and controller 400.
Wherein, accurate monochromatic and PLC technology LED panel 100 is used to produce multi-angle illumination, and after be radiated at simultaneously
On the circular hole that face is placed.Chromium plating is used for blocking illumination on a glass and the circular hole mask 200 with energy printing opacity circular hole is used to match somebody with somebody
Multi-angle illumination is closed, wherein, after light propagates certain distance through circular hole, there is the scanning of overlapping region on sample face.Phase
Machine detector 300 is used to gathering sample diffraction pattern under single exposure, and the distance sample of camera detector 300 it is default away from
From to reduce the aliasing of effective information under different illumination.The illumination pattern that controller 400 is used in Synchronization Control LED panel 100
Gathered with camera, to be imaged to the sample being arranged between circular hole mask 200 and camera detector 300, obtain and rebuild figure
Picture.The system 10 of the embodiment of the present invention can effectively improve the acquisition speed of no lens imaging system, realize single exposure
Without lens imaging, dynamic sample can be observed, it is simple easily to realize.
It is understood that the LED panel 100 of PLC technology, to realize while multi-angle illumination；Circular hole mask
200, to fit angle illumination, produce the scanning for having overlapping region on sample face；Camera detector 300, for gathering list
Sample diffraction pattern under secondary exposure；Controller 400, adopted to the illumination pattern in Synchronization Control LED panel 100 with camera
Collection.
Specifically, as shown in figure 1, the system 10 of the embodiment of the present invention mainly includes 4 parts：It is accurate monochromatic, programmable
The LED panel 100 of control, to produce multi-angle illumination, and is radiated on the circular hole placed below simultaneously.Circular hole mask 200,
Chromium plating is used for blocking illumination on a glass, the circular hole of an energy printing opacity is only left, to fit angle illumination.Light passes through circular hole
After propagating certain distance, there is the scanning of overlapping region on sample face.Camera detector 300, for gathering single exposure
Under sample diffraction pattern, camera detector 300 will be placed on distance sample place remote enough, have to reduce under different illumination
Imitate the aliasing of information.Controller 400, gathered to the illumination pattern in Synchronization Control LED panel 100 and camera.In addition, one
Thin sample is placed between circular hole and camera, as the object being observed.
Further, in one embodiment of the invention, selected section coherent light illumination and unit multiplication factor into
As system, there are the LED that 32*32 small cups are uniformly arranged, the wherein a length of 518nm of cardiac wave, spectral bandwidth 20nm in LED panel 100, and
The interval of adjacent LED lamp is 4mm.
It is understood that for without lens imaging, there are many design alternatives, for simplicity, select here
The imaging system of partially coherent optical illumination (i.e. narrow-band LED illuminates) and unit multiplication factor.There is 32* in the LED panel 100 used
32 LEDs uniformly arranged, the wherein a length of 518nm of cardiac wave, spectral bandwidth 20nm.The interval of adjacent LED lamp is 4mm.
Alternatively, in one embodiment of the invention, while 7*7 small cup LEDs are lighted, to provide multiplexed optical photograph, and often
Individual angular light is according to the directional light for a branch of partially coherent；A LED is lighted per adjacent 4 positions；Obtain more than 1.2*
1.2mm2Field range.
For example, the embodiment of the present invention lights 7*7 small cup lamps simultaneously, there is provided multiplexed optical is shone, and each angular light photograph can be seen
Into the directional light of a branch of partially coherent；A LED is lighted per adjacent 4 positions, and (namely the adjacent LED lighted is separated by
16mm)；Now, can obtain more than 1.2*1.2mm2Field range.When the timing of other specification one, while the LED lighted
Quantity is more, and the field range that can be obtained is bigger, but is finally limited by the size of detector.
Further, in one embodiment of the invention, under multi-angle illumination, each scanning on sample face
Pattern is all the fresnel diffraction pattern that circular hole propagates preset distance, and under the illumination of different angle, diffraction fresnel diffraction
Pattern produces different space displacements.
It is understood that under multi-angle illumination, each scanning pattern on sample face is that circular hole is propagated necessarily
The fresnel diffraction pattern of distance；And under different angular lights is shone, diffraction pattern produces different space displacements.Multi-angle light
According to effect, one is to provide enough constraints so that algorithm rebuilds more robust；The two be to be expanded into as
Field range.
Further, in one embodiment of the invention, on circular hole mask 200 size of circular hole according on sample
Mutual coverage information and aliasing information are made.
For example, suitably selection, the too small diffraction of radius can be needed too strong with the size of circular hole on circular hole mask 200
Strong and on sample mutual covering can very little；Radius, which crosses conference, causes the information of aliasing to be difficult to separate on the detector.Such as,
The embodiment of the present invention uses radius 300um circular hole.
Further, in one embodiment of the invention, pre-determined distance is determined according to the size of circular hole, with sample face
On scanning will meet the mutual overlay area of preparatory condition.
It is understood that on the basis of circle hole radius size determines, the spacing between suitable circular hole and sample is selected,
Scanning on sample face will have appropriate mutual overlay area, to provide enough constraints, for follow-up figure
As rebuilding.
Further, in one embodiment of the invention, camera detector 300 is monochromatic CMOS cameras, and pixel count is
2040*2448, single pixel size are 3.45um, most fast 35 frames of frame per second/second.
For example, camera detector 300, monochromatic CMOS cameras, pixel count 2040*2448, single pixel chi are used
Very little is 3.45um, most fast 35 frames of frame per second/second.The detector needs to be placed on the enough distant places of distance sample so that collects not
With pattern of the angular light according under, the effective coverage on imaging surface is sufficiently spaced-apart far, therefore has less information aliasing.
Alternatively, in one embodiment of the invention, in diffraction pattern, torn open by simple locus difference
Branch away to obtain the effective information under the illumination of different angle, and improve the quality of reconstruction image by optimized algorithm.
It is understood that in diffraction pattern, the effective information under different angle illumination, simple space bit can be passed through
Difference is put to split out.The distance that detector is placed is more remote, and information aliasing can be smaller, splits more accurate, the error meeting of reconstruction
It is smaller；But due to the limitation (place more remote, the signal collected is weaker) of signal to noise ratio, the demand of field range is considered further that, with
And resolution ratio is bigger as distance becomes big loss under partially coherent illumination, any distant place that camera detector 300 can not be placed,
Distance sample is actually placed on about at 15mm.Therefore, certain information aliasing can be produced, it is necessary to change by a kind of optimized algorithm
The quality of kind reconstruction image.
Further, the mark that the system 10 of the embodiment of the present invention is simple and easy, system is easily built and need not be complicated
The advantages that fixed and calibration, the quick no lens imaging of single exposure collection can be realized.For example, system can finally realize 35 frames/
Second (camera acquisition frame rate), more than 1.2*1.2mm2The dynamic imaging of visual field, 20um resolution ratio.The limitation of these indexs, can be with
By using the more preferable camera detector 300 of performance (such as smaller single pixel size, bigger effective search coverage, more
Fast frame per second and more preferable light efficiency) further improve.
Below to single principle of the exposure without lens imaging system 10 based on multi-angle illumination multiplexing of the embodiment of the present invention
It is illustrated, comprises the following steps：
S1：Gather the diffraction pattern under a no specimen using said system to be used to demarcate, while collection has sample pattern
(a static sample pattern or multiple dynamic sample patterns) are as the initial data rebuild；
S2：Using under no specimen, (content is that position is different, multiple diffraction for separately arranging for individual diffraction pattern of collection
Disc) plus certain threshold value demarcate effective information region corresponding to different illumination, and obtained by calculating to calibrate
The strength values of different illumination.
S3：According to calibration position above, the pattern that individual (or multiple) has sample is subjected to resolution, can be obtained
Obtain multiple images for carrying aliasing information；Rebuild with these data, one for observation sample information can be obtained and estimated
Evaluation, it is used as the initial value of optimized algorithm.
S4：Every collected has the diffraction pattern of sample, can regard as respectively using being spread out under multi-angle illumination
The intensity superposition of pattern is penetrated, therefore anti-effective information is solved using a kind of optimized algorithm.This algorithm is in one side reconstruction image
On the basis of, while the weight matrix under different illumination shared by information is calculated, continuously optimize the matrix.
S5：There is the diffraction image of sample using individual, bring above-mentioned initial value into, and rebuild with optimized algorithm, finally may be used
To obtain the complex amplitude information of sample.
To sum up, the system 10 of the embodiment of the present invention is simple and easy, system is easily built and need not be complicated demarcation and
Calibration.Based on single exposure of multi-angle illumination multiplexing without lens imaging, several LED are lighted using programmable LED array simultaneously
Lamp, there is provided multiple incident direction illumination (and Non-scanning mode) of multiplexing；The structure light is radiated at the circle of tens microns of size
Kong Shang, after propagating a segment distance, formed on sample face simultaneously, multizone, have overlapping scanning；Quilt after a segment distance is propagated again
Detector gathers.Because the parameter of light path is accurately set in advance, the aliasing very little of the information of multi-angle in single measurement；
And we devise a kind of method of global optimization, aliasing information can be separated by iteration weight, finally realize figure
As rebuilding.This method, on the basis of certain visual field and resolution ratio is sacrificed, can realize the quick no camera lens of individual collection into
Picture.Resolving power test target, static dyeing and pure phase sample, and the experimental result such as dynamic sample are we illustrated, this is analyzed with this
The performances such as field range, resolution ratio and the effect of method.
What is proposed according to embodiments of the present invention is exposed without lens imaging system based on the single of multi-angle illumination multiplexing, is easily taken
The demarcation and calibration built and need not be complicated, the quick no lens imaging of individual collection can be realized, so as to effectively improve nothing
The acquisition speed of lens imaging system, realize single exposure without lens imaging, dynamic sample can be observed, simply
Easily realize.
Exposed referring next to what accompanying drawing description proposed according to embodiments of the present invention based on the single of multi-angle illumination multiplexing without mirror
Head imaging method.
Fig. 3 is single flow of the exposure without lens imaging method based on multi-angle illumination multiplexing of one embodiment of the invention
Figure.
As shown in figure 3, it should be exposed based on the single of multi-angle illumination multiplexing without lens imaging method, using above-mentioned imaging system
System, wherein, method comprises the following steps：
In step S301, gather the diffraction pattern under no specimen and be used to demarcate, while collection has the pattern conduct of sample
The initial data of reconstruction.
In step s 302, effective information region corresponding to different illumination is demarcated plus predetermined threshold value by diffraction pattern,
And obtain to the strength values for the different illumination calibrated.
In step S303, the pattern for having sample is carried out directly according to effective information region corresponding to the different illumination of demarcation
Fractionation is connect, to obtain the image that multiple carry aliasing information.
In step s 304, the estimate for observation sample information is obtained according to multiple images with aliasing information,
To make the initial value of optimized algorithm, to pass through optimized algorithm.
In step S305, effective information is solved according to the initial value of optimized algorithm is counter, with the base of one side reconstruction image
On plinth, while the weight matrix under different illumination shared by information is calculated, continuously optimize weight matrix.
In step S306, there is the diffraction image of sample using individual, bring initial value into, and rebuild with optimized algorithm,
The complex amplitude information of sample is obtained, obtains reconstruction image.
Further, in one embodiment of the invention, have sample pattern include a static sample pattern or
Multiple dynamic sample patterns.
It should be noted that foregoing single solution of the exposure without lens imaging system embodiment to being multiplexed based on multi-angle illumination
Release explanation and be also applied for being exposed based on the list that multi-angle illumination is multiplexed without lens imaging method for the embodiment, it is no longer superfluous herein
State.
What is proposed according to embodiments of the present invention is exposed without lens imaging method based on the single of multi-angle illumination multiplexing, is easily taken
The demarcation and calibration built and need not be complicated, the quick no lens imaging of individual collection can be realized, so as to effectively improve nothing
The acquisition speed of lens imaging system, realize single exposure without lens imaging, dynamic sample can be observed, simply
Easily realize.
In the description of the invention, it is to be understood that term " " center ", " longitudinal direction ", " transverse direction ", " length ", " width ",
" thickness ", " on ", " under ", "front", "rear", "left", "right", " vertical ", " level ", " top ", " bottom " " interior ", " outer ", " up time
The orientation or position relationship of the instruction such as pin ", " counterclockwise ", " axial direction ", " radial direction ", " circumference " be based on orientation shown in the drawings or
Position relationship, it is for only for ease of and describes the present invention and simplify description, rather than indicates or imply that signified device or element must
There must be specific orientation, with specific azimuth configuration and operation, therefore be not considered as limiting the invention.
In addition, term " first ", " second " are only used for describing purpose, and it is not intended that instruction or hint relative importance
Or the implicit quantity for indicating indicated technical characteristic.Thus, define " first ", the feature of " second " can be expressed or
Implicitly include at least one this feature.In the description of the invention, " multiple " are meant that at least two, such as two, three
It is individual etc., unless otherwise specifically defined.
In the present invention, unless otherwise clearly defined and limited, term " installation ", " connected ", " connection ", " fixation " etc.
Term should be interpreted broadly, for example, it may be fixedly connected or be detachably connected, or integrally；Can be that machinery connects
Connect or electrically connect；Can be joined directly together, can also be indirectly connected by intermediary, can be in two elements
The connection in portion or the interaction relationship of two elements, limited unless otherwise clear and definite.For one of ordinary skill in the art
For, the concrete meaning of above-mentioned term in the present invention can be understood as the case may be.
In the present invention, unless otherwise clearly defined and limited, fisrt feature can be with "above" or "below" second feature
It is that the first and second features directly contact, or the first and second features pass through intermediary mediate contact.Moreover, fisrt feature exists
Second feature " on ", " top " and " above " but fisrt feature are directly over second feature or oblique upper, or be merely representative of
Fisrt feature level height is higher than second feature.Fisrt feature second feature " under ", " lower section " and " below " can be
One feature is immediately below second feature or obliquely downward, or is merely representative of fisrt feature level height and is less than second feature.
In the description of this specification, reference term " one embodiment ", " some embodiments ", " example ", " specifically show
The description of example " or " some examples " etc. means specific features, structure, material or the spy for combining the embodiment or example description
Point is contained at least one embodiment or example of the present invention.In this manual, to the schematic representation of above-mentioned term not
Identical embodiment or example must be directed to.Moreover, specific features, structure, material or the feature of description can be with office
Combined in an appropriate manner in one or more embodiments or example.In addition, in the case of not conflicting, the skill of this area
Art personnel can be tied the different embodiments or example and the feature of different embodiments or example described in this specification
Close and combine.
Although embodiments of the invention have been shown and described above, it is to be understood that above-described embodiment is example
Property, it is impossible to limitation of the present invention is interpreted as, one of ordinary skill in the art within the scope of the invention can be to above-mentioned
Embodiment is changed, changed, replacing and modification.
Claims (10)
1. a kind of exposed without lens imaging system based on the single of multi-angle illumination multiplexing, it is characterised in that including：
Accurate monochromatic and PLC technology LED panel, for producing multi-angle illumination, and being radiated at the circular hole placed below simultaneously
On；
Chromium plating is used for blocking illumination and with the circular hole mask of energy printing opacity circular hole on a glass, for coordinating the multi-angle light
According to, wherein, after light propagates certain distance through the circular hole, there is the scanning of overlapping region on sample face；
Camera detector, for gathering the sample diffraction pattern under single exposure, and the camera detector distance sample is pre-
If distance, to reduce the aliasing of effective information under different illumination；And
Controller, gathered for the illumination pattern described in Synchronization Control in LED panel and camera, to be covered to being arranged at the circular hole
Sample between mould and the camera detector is imaged, and obtains reconstruction image.
2. according to claim 1 exposed without lens imaging system based on the single of multi-angle illumination multiplexing, it is characterised in that
The imaging system of selected section coherent light illumination and unit multiplication factor, have what 32*32 small cups were uniformly arranged in the LED panel
LED, wherein cardiac wave a length of 518nm, spectral bandwidth 20nm, and the interval of adjacent LED lamp is 4mm.
3. according to claim 2 exposed without lens imaging system based on the single of multi-angle illumination multiplexing, it is characterised in that
7*7 small cup LEDs are lighted simultaneously, to provide multiplexed optical photograph, and each angular light is according to the directional light for a branch of partially coherent；Per adjacent
Light a LED in 4 positions；Obtain more than 1.2*1.2mm2Field range.
4. according to claim 1 exposed without lens imaging system based on the single of multi-angle illumination multiplexing, it is characterised in that
Under the multi-angle illumination, each scanning pattern on the sample face is the Fresnel that circular hole propagates preset distance
Diffraction pattern, and under the illumination of different angle, the diffraction fresnel diffraction pattern produces different space displacements.
5. according to claim 1 exposed without lens imaging system based on the single of multi-angle illumination multiplexing, it is characterised in that
The size of the circular hole is made according to mutual coverage information and the aliasing information on the sample on the circular hole mask.
6. according to claim 1 exposed without lens imaging system based on the single of multi-angle illumination multiplexing, it is characterised in that
The pre-determined distance is determined according to the size of the circular hole, the mutual of preparatory condition will be met with the scanning on the sample face
Overlay area.
7. according to claim 1 exposed without lens imaging system based on the single of multi-angle illumination multiplexing, it is characterised in that
The camera detector is monochromatic CMOS cameras, pixel count 2040*2448, and single pixel size is 3.45um, most fast frame per second
35 frames/second.
8. according to claim 4 exposed without lens imaging system based on the single of multi-angle illumination multiplexing, it is characterised in that
In the diffraction pattern, split out to obtain having under the illumination of the different angle by simple locus difference
Information is imitated, and improves the quality of the reconstruction image by optimized algorithm.
9. a kind of exposed without lens imaging method based on the single of multi-angle illumination multiplexing, it is characterised in that using such as claim
Imaging system described in any one of 1-8, wherein, it the described method comprises the following steps：
Diffraction pattern under collection no specimen is used to demarcate, while collection has the pattern of sample as the initial data rebuild；
Effective information region corresponding to different illumination is demarcated plus predetermined threshold value by diffraction pattern, and obtained to calibrate not
With the strength values of illumination；
The pattern for having sample is carried out by resolution according to effective information region corresponding to the different illumination of demarcation, with
Obtain multiple images for carrying aliasing information；
The estimate for observation sample information is obtained according to multiple described images with aliasing information, to make optimized algorithm
Initial value, to pass through optimized algorithm；
Effective information is solved according to the initial value of the optimized algorithm is counter, on the basis of the reconstruction image of one side, to calculate simultaneously
Go out the weight matrix shared by information under different illumination, continuously optimize the weight matrix；
There is the diffraction image of sample using individual, bring the initial value into, and rebuild with optimized algorithm, obtain the sample
Complex amplitude information, obtain reconstruction image.
10. single exposure according to claim 9 based on multi-angle illumination multiplexing exists without lens imaging method, its feature
In the pattern for having sample includes a static sample pattern or multiple dynamic sample patterns.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2020259042A1 (en) * | 2019-06-28 | 2020-12-30 | 江西视鼎科技有限公司 | Complex amplitude sensing imaging device and method |
| CN112697751A (en) * | 2020-12-07 | 2021-04-23 | 杭州电子科技大学 | Multi-angle illumination lens-free imaging method, system and device |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103984979A (en) * | 2014-06-06 | 2014-08-13 | 南昌航空大学 | Lens-diffraction-imaging-free automatic algae detection and counting device and method |
| CN104076620A (en) * | 2014-07-09 | 2014-10-01 | 东南大学 | Device and method for exposing volume hologram grating in one step |
-
2017
- 2017-12-20 CN CN201711385321.8A patent/CN107861360B/en active Active
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103984979A (en) * | 2014-06-06 | 2014-08-13 | 南昌航空大学 | Lens-diffraction-imaging-free automatic algae detection and counting device and method |
| CN104076620A (en) * | 2014-07-09 | 2014-10-01 | 东南大学 | Device and method for exposing volume hologram grating in one step |
Non-Patent Citations (2)
| Title |
|---|
| 代伟佳: "基于LED照明的无透镜散射成像方法研究", 《中国优秀硕士学位论文全文数据库》 * |
| 林炳文: "无透镜数字全息成像观测实验系统的研制", 《中国优秀硕士学位论文全文数据库》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2020259042A1 (en) * | 2019-06-28 | 2020-12-30 | 江西视鼎科技有限公司 | Complex amplitude sensing imaging device and method |
| CN112697751A (en) * | 2020-12-07 | 2021-04-23 | 杭州电子科技大学 | Multi-angle illumination lens-free imaging method, system and device |
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