CN107858787A - A kind of device for preparing used in tissue engineering composite biological material - Google Patents
A kind of device for preparing used in tissue engineering composite biological material Download PDFInfo
- Publication number
- CN107858787A CN107858787A CN201810003694.2A CN201810003694A CN107858787A CN 107858787 A CN107858787 A CN 107858787A CN 201810003694 A CN201810003694 A CN 201810003694A CN 107858787 A CN107858787 A CN 107858787A
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- CN
- China
- Prior art keywords
- receiver board
- tissue engineering
- spool
- biological material
- composite biological
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Classifications
-
- D—TEXTILES; PAPER
- D04—BRAIDING; LACE-MAKING; KNITTING; TRIMMINGS; NON-WOVEN FABRICS
- D04H—MAKING TEXTILE FABRICS, e.g. FROM FIBRES OR FILAMENTARY MATERIAL; FABRICS MADE BY SUCH PROCESSES OR APPARATUS, e.g. FELTS, NON-WOVEN FABRICS; COTTON-WOOL; WADDING ; NON-WOVEN FABRICS FROM STAPLE FIBRES, FILAMENTS OR YARNS, BONDED WITH AT LEAST ONE WEB-LIKE MATERIAL DURING THEIR CONSOLIDATION
- D04H1/00—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres
- D04H1/70—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres characterised by the method of forming fleeces or layers, e.g. reorientation of fibres
- D04H1/72—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres characterised by the method of forming fleeces or layers, e.g. reorientation of fibres the fibres being randomly arranged
- D04H1/728—Non-woven fabrics formed wholly or mainly of staple fibres or like relatively short fibres characterised by the method of forming fleeces or layers, e.g. reorientation of fibres the fibres being randomly arranged by electro-spinning
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- D—TEXTILES; PAPER
- D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
- D01D—MECHANICAL METHODS OR APPARATUS IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS
- D01D5/00—Formation of filaments, threads, or the like
- D01D5/0007—Electro-spinning
- D01D5/0061—Electro-spinning characterised by the electro-spinning apparatus
-
- D—TEXTILES; PAPER
- D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
- D01D—MECHANICAL METHODS OR APPARATUS IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS
- D01D5/00—Formation of filaments, threads, or the like
- D01D5/0007—Electro-spinning
- D01D5/0061—Electro-spinning characterised by the electro-spinning apparatus
- D01D5/0069—Electro-spinning characterised by the electro-spinning apparatus characterised by the spinning section, e.g. capillary tube, protrusion or pin
-
- D—TEXTILES; PAPER
- D01—NATURAL OR MAN-MADE THREADS OR FIBRES; SPINNING
- D01D—MECHANICAL METHODS OR APPARATUS IN THE MANUFACTURE OF ARTIFICIAL FILAMENTS, THREADS, FIBRES, BRISTLES OR RIBBONS
- D01D5/00—Formation of filaments, threads, or the like
- D01D5/0007—Electro-spinning
- D01D5/0061—Electro-spinning characterised by the electro-spinning apparatus
- D01D5/0076—Electro-spinning characterised by the electro-spinning apparatus characterised by the collecting device, e.g. drum, wheel, endless belt, plate or grid
-
- D—TEXTILES; PAPER
- D10—INDEXING SCHEME ASSOCIATED WITH SUBLASSES OF SECTION D, RELATING TO TEXTILES
- D10B—INDEXING SCHEME ASSOCIATED WITH SUBLASSES OF SECTION D, RELATING TO TEXTILES
- D10B2509/00—Medical; Hygiene
- D10B2509/06—Vascular grafts; stents
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- Engineering & Computer Science (AREA)
- Textile Engineering (AREA)
- Mechanical Engineering (AREA)
- Prostheses (AREA)
Abstract
A kind of device for preparing used in tissue engineering composite biological material, belong to tissue engineering material preparation facilities field, including material releasing unit and receiving unit, the receiving unit includes multiple general receiver board screens grooves, and multiple receiver boards that can be engaged with receiver board screens groove.The present invention can provide rich and varied lamellar structure by the receiving unit of setup module to prepare artificial blood vessel or scaffold matrices；Each receiving unit is arranged according to a graded, and by spool is wound each lamella one by one compound, diversified MULTILAYER COMPOSITE tissue engineering material can be easily obtained, greatly improve the preparation efficiency of MULTILAYER COMPOSITE tissue engineering material.
Description
Technical field
The invention belongs to tissue engineering material preparation facilities field, and in particular to one kind prepares used in tissue engineering compound bio
The device of material.
Background technology
Organizational project as one research, develop for repair, safeguard, promotes human body it is various organize or organ damage after
The new branch of science of the biosubstitute of function and form, its core are to establish the three dimensions being made up of cell and biomaterial to answer
Zoarium, its great advantage are can to form the vital biological tissue of tool, and form, the weight of 26S Proteasome Structure and Function are carried out to disease damage tissue
Build and reach permanent replacement；With minimum histocyte by cultivating amplification in vitro after, carry out bulk tissue defect and repair
It is multiple；Histoorgan defect situation random-shaping can be pressed, reaches perfect form reparation.
The research range of tissue engineering technique is extensive, at present in fields such as cartilage, bone, tendon, blood vessel, skin, corneas
Achieve gratifying progress.For example, in terms of preparation facilities, CN105671657A patents disclose one kind and are based on Static Spinning
The small-caliber artificial blood vessel preparation facilities of silk, the device are filled by high-voltage power apparatus for spinning solution injection device and reception
Offer power supply is provided, to form electrostatic field between the reception rod in the syringe needle and reception device in spinning solution injection device,
In addition, the patent also adjusts syringe needle and receives the spacing and angle offer facility between rod by injecting slide unit control device,
Receive and be threaded on rod, the artificial blood vessel that inwall carries helicla flute can be made.
Separately there are CN106012052A patents to provide one kind and combine biometric print and electrostatic spinning technique manufacture of intraocular blood
The device of pipe, the device include material releasing unit, Rotation of receiver unit, horizontal shifting platform, vertically move platform, high-tension electricity
Source and control unit.Material releasing unit includes biometric print nozzle and electrostatic spinning nozzle, biometric print nozzle and Static Spinning
Silk nozzle can work simultaneously, can also alternation, so as to prepare the artificial blood vessel with different structure.
However, above equipment is only used for preparing multilayer artificial blood vessel or the tubular bracket of individual layer or homogeneity, can not
Stratiform structure-rich changeable composite artificial blood vessel or timbering material are built, is brought greatly to cross-cutting research and production
Inconvenience.
The content of the invention
For above-mentioned the deficiencies in the prior art, the present invention is intended to provide a kind of used in tissue engineering composite biological material for preparing
Device.
To achieve the above object, this invention takes following technical scheme：One kind prepares used in tissue engineering compound bio material
The device of material, including material releasing unit and receiving unit, the receiving unit include multiple general receiver board screens grooves, with
And multiple receiver boards that can be engaged with receiver board screens groove.
Described device also includes frame, and telescoping shoring column, scalable branch are provided with outside the bottom land of receiver board screens groove
The other end of frame is arranged in frame.
Being horizontally disposed with the frame has a pair of parallel guide rail, and the multiple receiver board screens groove is horizontally set on two and led
Between rail；Spool is provided with above receiver board screens groove, perpendicular to guide rail direction, spool both ends are separately mounted to reeler
On, two reelers are slidably connected with two guide rails respectively.
On at least one reeler spool pressure sensor is provided with above spool.
Described device also includes cutting knife rest and installed in the cutting knife cut on knife rest, described to cut knife rest both ends difference
On two reelers.
The rotating cylinder for being axially perpendicular to receiver board screens groove bottom land, rotating cylinder one are provided with outside the bottom land of receiver board screens groove
End is connected with the bottom land external stability of the receiver board screens groove；Rotating cylinder periphery is provided with through ship tooth, and the gear teeth are moved towards parallel to rotating cylinder
Axially, the spur gear being meshed with the gear teeth on rotating cylinder is arranged in rotating shaft, the other end of rotating shaft and rack-mounted drive
Dynamic assembly axis are connected；The telescoping shoring column is telescopic bar, and expansion link is coaxially set in rotating cylinder with rotating cylinder.
Region includes spool berth area, reception area and curing area successively along guide rail direction between two guide rails, the multiple to connect
Receive board position groove and be distributed in reception area along guide rail direction is in one line, the curing area is provided with heater.
The material releasing unit includes head, multiple material storing box and the conveying passage connected respectively with material storing box, machine
Head is provided with nozzle, and nozzle is connected by channel converter with each conveying passage.
Crossbeam and cantilever are provided with the frame above guide rail, the crossbeam is set parallel to guide rail direction, cantilever
It is horizontally disposed with perpendicular to crossbeam direction, is slidably connected with crossbeam；The head is arranged on cantilever, is slidably connected with cantilever.
On the housiung separator ultraviolet source is provided with close to curing area.
Receiver board in the present invention can be the electrostatic spinning receiver board that surface engraving has specific microstructure, such as flat board knot
Structure, parallel stripes structure, lozenge structure, wavy micro-boss structure etc., Static Spinning is received using the receiver board of different structure
Silk, can obtain different functional layers.When spool receiver board surface scrolls by when, the piece that is received on Static Spinning filament plate
Shape functional layer is rolled to form tubular material；Make spool continue to roll through next receiver board, then can obtain double layered tubular
Composite；The like.When needing Rotation of receiver, start the rotating shaft drive device in frame, drive in rotating shaft and rotating shaft
Pinion rotation, the gear in rotating shaft further imparts power to rotating cylinder, driving receiver board screens groove rotation, can reach rotation
The purpose for receipts of transferring.
Receiver board can also be that surface is provided with the immersion liquid groove receiver board parallel to spool direction immersion liquid groove, be injected in groove special
After fixed solution or gel, immerse spool and rotated with given pace, tank liquid or with having been wrapped around on spool
Material reacts, or is attached to spool surface and forms tack coat or other specific layer structures.
After choosing suitable receiver board and receiver board being arranged on into receiver board screens groove according to certain order, hand-holdable spool
Operated.To save labour, further guide rail and reeler, spool both ends are set to be separately mounted on reeler, driving volume
Axle device starts reeler while movement along guide rail and drives spool to rotate, and the angle of winding can be precisely controlled, be wound
Tubular material it is smooth controllable.
The distance between receiver board and spool can be adjusted by telescoping shoring column, and be monitored by spool pressure sensor
Pressure between receiver board and spool, so as to control the degree of packing of winding.After windings reach requirement, start cutting knife and cut out
Remove excess sheet material.
After spool after reception area by obtaining MULTILAYER COMPOSITE tubulose, further pass through curing area, heated or UV-curing
Change can effectively lift the compound fastness of interlayer.
In view of the receiving unit of the present invention includes multiple receiving modules, accordingly, material releasing unit also possesses by not
With conveying the passage even function of different spray nozzles release different materials.Head in the present invention is provided with nozzle, and nozzle passes through logical
Road converter is connected from different conveying passages, can be achieved to draw raw material from different material storing box and discharges to the mesh of specified receiver board
's.The position of head can be by moving cantilever or head is slided on suspended wall to control.
Compared with prior art, the present invention can be to prepare artificial blood vessel or tubulose by the receiving unit of setup module
Timbering material provides rich and varied lamellar structure；Each receiving unit is arranged according to a graded, and will be each by spool
Lamella winds compound one by one, can easily obtain diversified MULTILAYER COMPOSITE tissue engineering material, greatly improve MULTILAYER COMPOSITE
The preparation efficiency of tissue engineering material.
Brief description of the drawings
Fig. 1 is the structural representation for the device that the embodiment of the present invention 1 prepares used in tissue engineering composite biological material；
Fig. 2 is Fig. 1 partial structural diagram；
Fig. 3 is the structural representation of receiver board screens slot part in Fig. 1；
Fig. 4 is the structural representation of wavy receiver board；
Fig. 5 is the structural representation of parallel stripes receiver board；
Fig. 6 is the structural representation of lozenge receiver board；
Fig. 7 is immersion liquid groove receiver board；
Fig. 8 is the cross section structure schematic diagram of three layers of compound support frame material in application examples 1.
In figure：1- head screens framves；2- crossbeams；3- cantilevers；4- conveying passages；5- heads；6- ultraviolet sources；7- frames；
8- cuts knife rest, 801- fix bars, 802- guide rail poles；9- cutting knife；10- spools；11- spool pressure sensors；12- walking teeth
Wheel；13- receiver board screens grooves；14- guide rails；15- heaters；16- reelers；17- material storing box；18- controllers；19- racks；
20- chutes；21- sliding blocks；22- guide rail pole electric rotating machines；23- spool electric rotating machines；24- rotating shafts；25- telescoping shoring columns；26- turns
Cylinder；27- spur gears；The wavy receiver boards of 28-；29- parallel stripes receiver boards；30- lozenge receiver boards；31- immersion liquid groove receives
Plate；32- immersion liquid grooves；33- endothecium structures；34- interlayer structures；35- layer structures.
Embodiment
The present invention is elaborated with reference to specific embodiment.
Embodiment 1
A kind of device for preparing used in tissue engineering composite biological material, as Figure 1-3, including frame 7, material releasing unit and
Receiving unit.
Being horizontally disposed with frame 7 has a pair of parallel guide rail 14, and region is empty successively along the direction of guide rail 14 between two guide rails 14
Plan is divided into spool berth area, reception area and curing area, forms flowing water station.
Receiving unit, which includes three general receiver board screens grooves 13 and five, to be engaged with receiver board screens groove 13
Receiver board, one of receiver board is optic panel, and remaining four receive harden structures as shown in figs. 4-7, and wherein shown in Fig. 4 is
Wavy receiver board 28, shown in Fig. 5 is parallel stripes receiver board 29, and shown in Fig. 6 is lozenge receiver board 30, shown in Fig. 7
Be immersion liquid groove receiver board 31.Three receiver board screens grooves 13 along the direction of guide rail 14 it is in one line distribution, be horizontally set on reception
Area.The rotating cylinder 26 for being axially perpendicular to the bottom land of receiver board screens groove 13, rotating cylinder are provided with outside the bottom land of receiver board screens groove 13
26 upper ends are connected with the bottom land external stability of receiver board screens groove 13；The periphery of rotating cylinder 26 is provided with through ship tooth, and through ship tooth moves towards parallel
In rotating cylinder 26 axially, the spur gear 27 being meshed with the through ship tooth on rotating cylinder 26 is arranged in rotating shaft 24, the other end of rotating shaft 24
It is connected with the rotating shaft drive motor shaft in frame 7.Coaxial sleeve is provided with telescoping shoring column 25, the scalable branch in rotating cylinder 26
With being connected outside the bottom land of receiver board screens groove 13, the other end is arranged in frame 7 for the upper end of frame 25.The telescoping shoring column 25 is
Telescopic bar, it can relatively rotate between sleeve.Rotating shaft motor electrically connects with expansion link control unit with controller 18.
Spool 10 is provided with above receiver board screens groove 13, perpendicular to the direction of guide rail 14, the both ends of spool 10 are installed respectively
On reeler 16, it is in inverse-T-shaped sliding block 21 that the bottom of two reeler 16, which is respectively equipped with shape of cross section, the upper part of two guide rail 14
The chute 20 corresponding with the shape of sliding block 21 is not provided with, and reeler 16 is by being arranged on the sliding block 21 of its underpart and being arranged on guide rail
The chute 20 on top is slidably connected with guide rail 14.
On two reelers 16, walking gear 12 is housed towards the side of relative reeler 16；On two guide rails 14, towards relative
The side of guide rail 14 is equipped with rack 19, and the trend of rack 19 moves towards consistent with guide rail 14；Walking gear positioned at spool 10 with one end
12 engage with rack 19.Walking gear 12 on two reelers 16 is driven by synchronous clockwise and anticlockwise motor, synchronous rotating
The power supply of motor electrically connects with controller 18.
A structure of an essay for two reelers 16 cuts knife rest 8, cuts fix bar 801 and guide rail pole that knife rest 8 includes be arrangeding in parallel
802, guide rail pole 802 is provided with screw thread, and cutting knife 9 is set in fix bar 801 and guide rail pole 802, cutting knife 9 and fix bar
801 are slidably connected, are threadedly coupled with guide rail pole 802.The reeler 16 of the one end of guide rail pole 802 is provided with guide rail pole electric rotating machine
22。
Spool electric rotating machine 13 is also equipped with two reelers 16；It is provided with one of reeler 16 positioned at the top of spool 10
Spool pressure sensor 11.
The power supply of guide rail pole electric rotating machine 22, the power supply of spool electric rotating machine 23 and spool pressure sensor 11 with controller 18
Electrical connection.
Head screens frame 1 is provided with above the area of spool berth in frame 7, head screens frame 1 is provided with three head cards
Position groove.
Crossbeam 2 and cantilever 3 are provided with positioned at the top of guide rail 14 in frame 7, crossbeam 2 is set parallel to the direction of guide rail 14, cantilever 3
It is horizontally disposed with perpendicular to the direction of crossbeam 2, is slidably connected with crossbeam 2.Material releasing unit includes three heads 5（Only drawn in figure
1）With 6 material storing box 17, each head 5 is connected with each material storing box 17, set on head 5 respectively by six conveying passages 4
There is nozzle, nozzle is connected by channel converter with each conveying passage 4.One of head 4 is arranged on cantilever 3, with cantilever 3
It is slidably connected.Two other head 5（It is not drawn into figure）Card is placed on standby in head screens groove.It is respectively quiet on three heads 5
Electric spinning spray nozzle, 3D printing nozzle, common feed introduction.
Curing area is provided with heater 15；On the crossbeam 2 of frame 7 ultraviolet source 6 is provided with close to curing area.Receiver board screens groove
Ultrasonic transducer is provided with 13（It is not drawn into figure）.
Below exemplified by preparing three layers of timbering material as shown in Figure 8, the operation principle of the present invention is illustrated.
Application examples 1
For convenience of description, first material storing box 17 is numbered, marks 1#, 2#, 3#, 4#, 5#, 6# successively.
Using volume ratio as 7:3 dichloromethane, dimethylformamide mixed solution are solvent, prepare the poly- of 10wt% concentration
Lactic acid（PLA）Spinning solution, add 1# material storing box 17；
Using volume ratio as 7:3 dichloromethane, dimethylformamide mixed solution are solvent, compound concentration 8wt% polylactic acid poly
Caprolactone block copolymer（PLCL）Spinning solution, add 2# material storing box 17；
Using volume ratio as 7:3 dichloromethane, dimethylformamide mixed solution are solvent, prepare the gathering in oneself of 14wt% concentration
Ester（PCL）Spinning solution, add 3# material storing box 17.
Selection with electrostatic spinning nozzle head 5 be arranged on cantilever 3 on, electrostatic spinning nozzle by channel converter,
Three conveying passages 4 connect with 1-3# material storing box 17 respectively.
Before start, spool 10 is docked in positioned at the spool berth area of the one end of guide rail 14（Position shown in Fig. 1）.Retouch for convenience
State, the receiver board screens groove 13 nearest apart from spool berth area is labeled as the first receiver board screens groove, slightly remote mark is
Two receiver board screens grooves, farthest mark are three receiver board screens grooves.Parallel stripes are installed in the first receiver board screens groove
Receiver board 29, make stripe direction parallel to spool 10 during installation；Wavy receiver board 28 is installed in the second receiver board screens groove,
Make wave trend during installation parallel to guide rail direction；Lozenge receiver board 30 is installed in the 3rd receiver board screens groove.
Spool pressure parameter is set beforehand through controller 18.
Channel converter 11 is adjusted, electrostatic spinning nozzle is connected with 1# material storing box 17；Cantilever 3 slides along crossbeam 2, head
5 slide along cantilever 3, the head 5 with electrostatic spinning nozzle is moved to the top of parallel stripes receiver board 29, open nozzle and carry out
First time electrostatic spinning, the silk of ejection are received on parallel stripes receiver board 29.Then synchronous positive and negative motor driving walking is started
Gear 12 rotates forward along rack 19, and then drives reeler 16 along the forward slip of guide rail 14, while starts spool electric rotating machine
23 drive spools 10 roll（The rotating direction of spool 10 is consistent with the rotation direction of walking gear 12）, when spool 10 is in parallel stripes
Roll by receiving the fibrous layer on parallel stripes receiver board 29 and being wound onto outside spool 10 to be cylindrical in shape the top of receiver board 29.
Start guide rail pole electric rotating machine 22, driving guide rail pole 802 rotates, and then drives cutting knife 9 to be translated along guide rail pole 802, will be unnecessary
Fibre cutting, obtain the endothecium structure 33 of timbering material.
Spool 10 makes cantilever 3 drive head 5 along the forward slip of crossbeam 2 when rolling winding on parallel stripes receiver board 29
Until the row of head 5 to the wavy top of receiver board 28.Channel converter 11 is adjusted, electrostatic spinning nozzle is connected with 2# material storing box 17
It is logical, carry out second of electrostatic spinning.After the wavy reception of receiver board 28 terminates, cantilever 3 drives head 5 to continue forward slip to water chestnut
The top of shape line receiver board 30, switching channel converter 11 make electrostatic spinning nozzle be connected with 3# material storing box 17, and it is quiet to start third time
Electrospun.Now reeler 16 drives spool to be moved to the wavy top of receiver board 28, and spool rotates, and will receive wavy connect
Fiber on receipts plate 28, which rolls, to be wound up into rotating shaft, is wrapped in outside endothecium structure 33, cut knife 9 is arranged and cut after the completion of winding
Excess fiber is removed, obtains the interlayer structure 34 of timbering material.
Likewise, after third time electrostatic spinning terminates, reeler 16 drives spool to be moved on lozenge receiver board 30
Side, spool 10 rotate, the fibre sheet material received on lozenge receiver board 30 are wound up into rotating shaft, is wrapped in interlayer structure
Cut knife 9, which arranges, outside 34, after the completion of winding cuts excess fiber, forms layer structure 35.
When spool 10 rolls from receiver board to be passed through, spool pressure sensor 11 gathers the pressure data of spool 10 and by number
According to controller 18 is sent to, controller 18 is contrasted the pressure value collected with setting spool pressure parameter, judges to press
Whether power meets sets requirement.If hypertonia, shorten telescoping shoring column 25 starting expansion link control unit, connect with reducing
The height of board position groove 13 is received, reduces the pressure between spool 10 and receiver board；If insufficient pressure, starting, expansion link control is single
Member extends telescoping shoring column 25, to lift the height of receiver board screens groove 13, increases the pressure between spool 10 and receiver board.
After the completion of winding, spool 10 is set to continue to move along to the top of heater 15, to the material on spool 10
Material carries out heating fixed line, thoroughly flings to solvent.The cylindrical material being wound into is removed from spool 10 after cooling, obtains PLA/
Tri- layers of compound support frame materials of PLCL/PCL.
Application examples 2：
As another application mode, it is with the difference of application examples 1：
Parallel stripes receiver board 29 in the first receiver board screens groove is replaced with into optic panel.Electrostatic is received in optic panel
During spinning fibre, opening of rotary shaft motor, it is allowed to drive shaft 24 and rotates, and then drives and be connected with the gear of rotating shaft 24
Rotating cylinder 26 rotate, the first receiver board screens groove installed in the upper end of rotating cylinder 26 rotates in the horizontal plane therewith, so that smooth surface
Plate plays a part of Rotation of receiver.
Application examples 3：
As another application mode, it is with the difference of application examples 1：
Wavy receiver board 28 in the second receiver board screens groove is replaced with into the immersion liquid groove with immersion liquid groove 32 to receive
Plate 31.Hydrogel material is added in 4# material storing box 17.
After first time electrostatic spinning terminates, the head 5 with electrostatic spinning nozzle is laid down, by with common feed introduction
Head, 5 hang under cantilever 3, and nozzle location is in the top of immersion liquid groove 32.Switching channel converter, makes nozzle pass through conveying
Passage 4 is connected with 4# material storing box 17, opens nozzle and head 5 is moved along cantilever 3, hydrogel material is discharged in immersion liquid groove 32
In.
Spool 10 is moved to the top of immersion liquid groove 32 simultaneously, expansion link control unit is started by controller 18, driving can
Telescope support 25 is extended, and the immersion liquid groove height lifting installed in the upper end of telescoping shoring column 25, spool 10 is sunk in immersion liquid groove 32 simultaneously
A period of time is rolled in the situ of immersion liquid groove 32, hydrogel material is uniformly wrapped up outside spool, subsequently into subsequent processing.For
Hydrogel material is set quickly to be soaked on endothecium structure surface even, spool 10 is opened during the situ of immersion liquid groove 32 rolls and is arranged on the
Ultrasonic transducer in two receiver board screens grooves.Heating stepses omit, and other steps are constant, obtain PLA/ hydrogels/PCL tri-
Layer compound support frame material.
If raw material includes UV fixed line materials, when spool 10 is moved to curing area, ultraviolet source 6 can be opened material is carried out
Solidification.
It the above is only a part of application mode of the present invention, in practice, the parameter such as change raw material, layer number, Rotating fields can
Easily to provide more assembled schemes.Using the device, with reference to prior art conversion type of feed, receiver board order is adjusted,
It is a collection of to prepare multigroup sample, comprehensive assessment is carried out on this basis, can quickly and efficiently filter out excellent performance
Tissue engineering material, greatly improve test efficiency.
Claims (10)
1. a kind of device for preparing used in tissue engineering composite biological material, including material releasing unit and receiving unit, its feature
It is, the receiving unit includes multiple general receiver board screens grooves, and multiple can be engaged with receiver board screens groove
Receiver board.
2. the device of used in tissue engineering composite biological material is prepared as claimed in claim 1, it is characterised in that described device is also wrapped
Including rack, telescoping shoring column is provided with outside the bottom land of receiver board screens groove, the other end of telescoping shoring column is arranged on frame
On.
3. the device of used in tissue engineering composite biological material is prepared as claimed in claim 2, it is characterised in that water in the frame
Flat to be provided with a pair of parallel guide rail, the multiple receiver board screens groove is horizontally set between two guide rails；Positioned at reception board
Spool is provided with above the groove of position, perpendicular to guide rail direction, spool both ends are separately mounted on reeler, and two reelers are led with two respectively
Rail is slidably connected.
4. the device of used in tissue engineering composite biological material is prepared as claimed in claim 3, it is characterised in that at least one spool
On device spool pressure sensor is provided with above spool.
5. the device of used in tissue engineering composite biological material is prepared as claimed in claim 3, it is characterised in that described device is also wrapped
Include and cut knife rest and be separately mounted to installed in the cutting knife cut on knife rest, the knife rest both ends that cut on two reelers.
6. the device of used in tissue engineering composite biological material is prepared as described in claim 2-5 is any, it is characterised in that positioned at connecing
Receive the rotating cylinder for being provided with outside the bottom land of board position groove and being axially perpendicular to receiver board screens groove bottom land, rotating cylinder one end and the receiver board
The bottom land external stability of screens groove is connected；Rotating cylinder periphery is provided with through ship tooth, and gear teeth trend is parallel to rotating cylinder axial direction, and on rotating cylinder
The spur gear that the gear teeth are meshed is arranged in rotating shaft, and the other end of rotating shaft is connected with rack-mounted driving unit shaft；Institute
It is telescopic bar to state telescoping shoring column, and expansion link is coaxially set in rotating cylinder with rotating cylinder.
7. the device of used in tissue engineering composite biological material is prepared as claimed in claim 6, it is characterised in that area between two guide rails
Domain includes spool berth area, reception area and curing area successively along guide rail direction, and the multiple receiver board screens groove is along guide rail direction
In one line to be distributed in reception area, the curing area is provided with heater.
8. preparing the device of used in tissue engineering composite biological material as described in claim 1 or 2 or 3 or 4 or 5 or 7, its feature exists
In the material releasing unit includes head, multiple material storing box and the conveying passage connected respectively with material storing box, is set on head
There is nozzle, nozzle is connected by channel converter with each conveying passage.
9. the device of used in tissue engineering composite biological material is prepared as claimed in claim 8, it is characterised in that the frame is upper
Crossbeam and cantilever are provided with above guide rail, the crossbeam is set parallel to guide rail direction, and cantilever vertical is set in crossbeam direction level
Put, be slidably connected with crossbeam；The head is arranged on cantilever, is slidably connected with cantilever.
10. the device of used in tissue engineering composite biological material is prepared as claimed in claim 9, it is characterised in that the frame is horizontal
On beam ultraviolet source is provided with close to curing area.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810003694.2A CN107858787B (en) | 2018-01-03 | 2018-01-03 | Device for preparing composite biological material for tissue engineering |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201810003694.2A CN107858787B (en) | 2018-01-03 | 2018-01-03 | Device for preparing composite biological material for tissue engineering |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN107858787A true CN107858787A (en) | 2018-03-30 |
| CN107858787B CN107858787B (en) | 2023-09-22 |
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ID=61707755
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| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201810003694.2A Active CN107858787B (en) | 2018-01-03 | 2018-01-03 | Device for preparing composite biological material for tissue engineering |
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Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
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| CN207775491U (en) * | 2018-01-03 | 2018-08-28 | 郑州大学 | A kind of device preparing used in tissue engineering composite biological material |
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| US8124001B1 (en) * | 2008-05-21 | 2012-02-28 | Clemson University Research Foundation | Synthetic vascular tissue and method of forming same |
| US20120301567A1 (en) * | 2010-02-05 | 2012-11-29 | Contipro Biotech S.R.O. | Apparatus for production of two-dimensional or three-dimensional fibrous materials of microfibres and nanofibres |
| CN103320877A (en) * | 2013-07-09 | 2013-09-25 | 苏州大学 | Method for preparing degradable tissue engineering three-dimensional support and device for machining degradable tissue engineering three-dimensional support |
| CN103977457A (en) * | 2014-05-30 | 2014-08-13 | 国家纳米科学中心 | Tubular tissue engineering scaffold and preparation method thereof |
| CN104862787A (en) * | 2015-05-19 | 2015-08-26 | 上海大学 | System and method for partition electrospinning of multiple materials |
| CN105568403A (en) * | 2016-01-27 | 2016-05-11 | 广东工业大学 | Centrifugal electrostatic spinning device with rotary air suction function |
| CN106012052A (en) * | 2016-08-03 | 2016-10-12 | 苏州大学附属第二医院 | Device for manufacturing artificial blood vessel through combination of bio-printing and electro-spinning technologies |
| CN106584836A (en) * | 2016-11-30 | 2017-04-26 | 广州博敏科技有限公司 | Composite 3D printing forming system, forming method and intravascular stent |
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