CN107857794B - Protein dialysis device and system - Google Patents
Protein dialysis device and system Download PDFInfo
- Publication number
- CN107857794B CN107857794B CN201711403625.2A CN201711403625A CN107857794B CN 107857794 B CN107857794 B CN 107857794B CN 201711403625 A CN201711403625 A CN 201711403625A CN 107857794 B CN107857794 B CN 107857794B
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- dialysis
- protein
- wheel
- wheel shaft
- rechargeable battery
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- 238000000502 dialysis Methods 0.000 title claims abstract description 143
- 102000004169 proteins and genes Human genes 0.000 title claims abstract description 62
- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 62
- PXHVJJICTQNCMI-UHFFFAOYSA-N Nickel Chemical compound [Ni] PXHVJJICTQNCMI-UHFFFAOYSA-N 0.000 claims description 4
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 3
- WHXSMMKQMYFTQS-UHFFFAOYSA-N Lithium Chemical compound [Li] WHXSMMKQMYFTQS-UHFFFAOYSA-N 0.000 claims description 3
- HBBGRARXTFLTSG-UHFFFAOYSA-N Lithium ion Chemical compound [Li+] HBBGRARXTFLTSG-UHFFFAOYSA-N 0.000 claims description 3
- 229910018487 Ni—Cr Inorganic materials 0.000 claims description 3
- VNNRSPGTAMTISX-UHFFFAOYSA-N chromium nickel Chemical compound [Cr].[Ni] VNNRSPGTAMTISX-UHFFFAOYSA-N 0.000 claims description 3
- 229910021389 graphene Inorganic materials 0.000 claims description 3
- 229910052739 hydrogen Inorganic materials 0.000 claims description 3
- 239000001257 hydrogen Substances 0.000 claims description 3
- 229910052744 lithium Inorganic materials 0.000 claims description 3
- 229910001416 lithium ion Inorganic materials 0.000 claims description 3
- 229920000642 polymer Polymers 0.000 claims description 3
- 230000001105 regulatory effect Effects 0.000 claims description 3
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 claims description 2
- 229910052759 nickel Inorganic materials 0.000 claims description 2
- 239000012535 impurity Substances 0.000 abstract description 5
- 238000002474 experimental method Methods 0.000 abstract description 2
- 239000007853 buffer solution Substances 0.000 description 10
- 239000000872 buffer Substances 0.000 description 6
- 239000000243 solution Substances 0.000 description 4
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical class N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 238000001742 protein purification Methods 0.000 description 3
- 238000007789 sealing Methods 0.000 description 3
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 2
- 235000011130 ammonium sulphate Nutrition 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 239000003381 stabilizer Substances 0.000 description 2
- 230000000087 stabilizing effect Effects 0.000 description 2
- 239000006228 supernatant Substances 0.000 description 2
- 235000019750 Crude protein Nutrition 0.000 description 1
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000004113 cell culture Methods 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 238000000605 extraction Methods 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000008188 pellet Substances 0.000 description 1
- 239000012466 permeate Substances 0.000 description 1
- 239000008363 phosphate buffer Substances 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/34—Extraction; Separation; Purification by filtration, ultrafiltration or reverse osmosis
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Biochemistry (AREA)
- Analytical Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Water Supply & Treatment (AREA)
- External Artificial Organs (AREA)
Abstract
The invention relates to the technical field of biological experiment devices, in particular to a protein dialysis device and a protein dialysis system. The protein dialysis device comprises a driving device and a dialysis rotating frame; the driving device is used for driving the dialysis rotating frame to rotate; the dialysis rotating frame is used for bearing a dialysis bag, wherein the dialysis rotating frame comprises a wheel shaft and a wheel fan; the wheel fan is arranged on the wheel shaft through a spoke, the spoke is arranged along the radial direction of the wheel shaft, and the driving device drives the wheel shaft to rotate. The protein dialysis system comprises the protein dialysis device. The invention can avoid the conditions that the sample leaks and a plurality of dialysis bags are mutually wound when the magnetic stirrer is used and the dialysis bags are damaged, and can accelerate the permeation of micromolecular proteins or other impurities in the dialysis bags so as to achieve the aim of purifying the proteins.
Description
Technical Field
The invention relates to the technical field of biological experiment devices, in particular to a protein dialysis device and a protein dialysis system.
Background
At present, most of protein dialysis is to put a dialysis bag into dialysis buffer solution, and then use a magnetic stirrer to actuate a magnetic rotor to rotate so as to enable the dialysis buffer solution to form vortex, thereby promoting the permeation of small molecular proteins or other impurities in the dialysis bag, retaining target macromolecular proteins and achieving the purpose of protein purification. The use of magnetic stirrers often presents problems during use, such as: sealing with a clamp or tying a dialysis bag with a rope, wherein the magnetic rotor and the sealing clamp can collide with each other to cause loosening, so that a sample leaks; when a plurality of samples are dialyzed, the dialysis bags can be mutually wound, and the dialysis effect is affected; the dialysis bag may also be damaged when the magnetic rotor speed is improperly adjusted.
Disclosure of Invention
The invention aims to provide a protein dialysis device and a protein dialysis system, which are used for solving the technical problems that during protein dialysis in the prior art, samples are easy to leak, a plurality of dialysis bags are mutually wound, and the dialysis bags are damaged.
The invention provides a protein dialysis device, which comprises a driving device and a dialysis rotating frame; the driving device is used for driving the dialysis rotating frame to rotate; the dialysis rotating frame is used for bearing a dialysis bag, wherein the dialysis rotating frame comprises a wheel shaft and a wheel fan; the wheel fan is arranged on the wheel shaft through a spoke, the spoke is arranged along the radial direction of the wheel shaft, and the driving device drives the wheel shaft to rotate.
Further, a fixed clamp assembly is arranged on the dialysis rotating frame and used for clamping the dialysis bag.
Further, the wheel fan comprises a frame strip; the frame strips extend along the axial direction of the wheel shaft; one end of the spoke is connected with the frame strip, and the other end of the spoke is connected with the wheel shaft.
Further, still include the apron, drive arrangement install in on the apron, just drive arrangement with dialysis rotating turret is located respectively the two different faces of apron.
Further, a speed regulator is also included for regulating the rotational speed of the drive.
Further, a battery is also included for providing power to the drive device.
Further, the battery is a rechargeable battery.
Further, the rechargeable battery is a polymeric lithium ion rechargeable battery, a nickel-chromium rechargeable battery, a nickel-hydrogen rechargeable battery, a lithium polymer rechargeable battery or a graphene rechargeable battery.
Further, the driving device is a motor.
The invention also provides a protein dialysis system, which comprises the protein dialysis device.
Compared with the prior art, the invention has the beneficial effects that:
the protein dialysis device provided by the invention comprises a driving device and a dialysis rotating frame; the driving device is used for driving the dialysis rotating frame to rotate; the dialysis rotating frame is used for bearing a dialysis bag, wherein the dialysis rotating frame comprises a wheel shaft and a wheel fan; the wheel fan is arranged on the wheel shaft through a spoke, the spoke is arranged along the radial direction of the wheel shaft, and the driving device drives the wheel shaft to rotate. Through installing dialysis bag on dialysis rotating turret to drive dialysis rotating turret through drive arrangement and rotate, just so can realize that the board bag that permeates also rotates, thereby also can make the dialysis buffer produce the motion, when both can avoid using magnetic stirrer like this, the sample is leaked, twines each other between a plurality of dialysis bags, and the condition that the damage can appear in the dialysis bag takes place, can accelerate the showthrough of micromolecular protein or other impurity in the dialysis bag again, reaches the purpose of protein purification.
The invention also provides a protein dialysis system, which comprises the protein dialysis device. Based on the analysis, the protein dialysis system can avoid the conditions that the sample leaks and a plurality of dialysis bags are mutually wound and damaged when the magnetic stirrer is used, and can accelerate the permeation of micromolecular proteins or other impurities in the dialysis bags, thereby achieving the purpose of purifying the proteins.
Drawings
In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings that are needed in the description of the embodiments or the prior art will be briefly described, and it is obvious that the drawings in the description below are some embodiments of the present invention, and other drawings can be obtained according to the drawings without inventive effort for a person skilled in the art.
FIG. 1 is a schematic diagram of a protein dialysis apparatus according to an embodiment of the present invention;
FIG. 2 is an enlarged schematic view of a portion of FIG. 1 at A;
FIG. 3 is a schematic diagram showing a structure of a protein dialysis apparatus according to an embodiment of the present invention at another view angle;
FIG. 4 is an enlarged partial schematic view at B in FIG. 3;
fig. 5 is a schematic structural view of a second fixing clip according to a first embodiment of the present invention.
In the figure:
101-an electric motor; 102-wheel axle; 103-spokes; 104-frame bars; 105-a first fixation clamp; 106-a second fixation clamp; 107-third half; 108-fourth half; 110-a second groove; 111-second protrusions; 115-locking bolt; 116-tightening the screw; 117-cover plate; 118-non-slip mat; 119-speed regulator; 120-cell; 121-guide bar.
Detailed Description
The following description of the embodiments of the present invention will be made apparent and fully in view of the accompanying drawings, in which some, but not all embodiments of the invention are shown. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
In the description of the present invention, it should be noted that the directions or positional relationships indicated by the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc. are based on the directions or positional relationships shown in the drawings, are merely for convenience of describing the present invention and simplifying the description, and do not indicate or imply that the devices or elements referred to must have a specific orientation, be configured and operated in a specific orientation, and thus should not be construed as limiting the present invention. Furthermore, the terms "first," "second," and "third" are used for descriptive purposes only and are not to be construed as indicating or implying relative importance.
In the description of the present invention, it should be noted that, unless explicitly specified and limited otherwise, the terms "mounted," "connected," and "connected" are to be construed broadly, and may be either fixedly connected, detachably connected, or integrally connected, for example; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium, and can be communication between two elements. The specific meaning of the above terms in the present invention will be understood in specific cases by those of ordinary skill in the art.
Example 1
Referring to fig. 1 to 5, a first embodiment of the present invention provides a protein dialysis apparatus, which includes a driving device and a dialysis turret; the driving device is used for driving the dialysis rotating frame to rotate; the dialysis rotating frame is used for bearing a dialysis bag, wherein the dialysis rotating frame comprises a wheel shaft 102 and a wheel fan; the wheel fan is arranged on the wheel axle 102 through the wheel spokes 103, the wheel spokes 103 are arranged along the radial direction of the wheel axle 102, and the driving device drives the wheel axle 102 to rotate.
According to the protein dialysis device provided by the embodiment, the dialysis bag is arranged on the dialysis rotating frame, and the dialysis rotating frame is driven to rotate by the driving device, so that the transparent plate bag can also rotate, and the dialysis buffer solution can also move; the protein dialysis device provided by the embodiment discards the way that the magnetic stirrer stirs the dialysis buffer; the dialysis bag is fixed on the dialysis rotating frame and drives the driving device to drive the dialysis rotating frame to rotate, so that the possibility of loosening the sealing of the sample dialysis bag can be reduced, and the sample device is prevented from leaking; in addition, as the dialysis bags are fixed on the dialysis rotating frame, the situation that a plurality of dialysis bags are mutually wound can be avoided to a certain extent, and the occurrence of damage to the dialysis bags can be avoided; because the dialysis bag can rotate and the dialysis buffer solution can be stirred, the permeation of small molecular proteins or other impurities in the dialysis bag can be accelerated, and the purpose of protein purification is achieved.
In an alternative to this embodiment, the wheel fan includes a rim strip 104; the frame strip 104 extends along the axial direction of the wheel axle 102, i.e. the length direction of the frame strip 104 is parallel to the axial direction of the wheel axle 102; one end of the spoke 103 is fixedly connected with the frame strip 104, and the other end of the spoke 103 is fixedly connected with the wheel axle 102.
Specifically, the number of the wheel fans is plural, and the plural wheel fans are uniformly distributed along the circumferential direction of the wheel axle 102 so that the wheel axle 102 can smoothly rotate. The number of rim strips 104 in each wheel fan is two, and the two rim strips 104 in each wheel fan are symmetrical about the axis of the axle 102. In this embodiment, the number of the wheel fans is taken as two as an example; the two wheel fans are arranged in a cross shape, namely, the central angle between two adjacent frame strips 104 is 90 degrees.
In an alternative scheme of the embodiment, the frame strip 104 is fixedly connected with the wheel axle 102 through two spokes 103 which are arranged in parallel at intervals, and the two spokes 103 are arranged up and down; the length direction of the spokes 103 is perpendicular to the axis of the axle 102.
In an alternative of this embodiment, the dialysis turret is provided with a retaining clip assembly for clipping the dialysis bag. A clip assembly is provided on each of the frame strips 104.
Specifically, the clip assembly includes a first clip 105 and a second clip 106, the second clip 106 being located above the first clip 105.
The first fixing clip 105 comprises a first half provided with a first recess and a second half having a first protrusion cooperating with the first recess, the first protrusion being insertable into the first recess for enabling the lower end of the dialysis bag to be clamped between the first half and the second half. Between the first half and the second half is a locking bolt 115 for enabling the first protrusion to remain inserted into the first recess. The first fixing clip 105 is fixed to the spoke 103 located below. The second fixing clip 106 is slidable along the length direction of the frame strip 104.
The second fixation clamp 106 comprises a third half 107 and a fourth half 108, the third half 107 being provided with a second recess 110, the fourth half having a second protrusion 111 cooperating with the second recess 110, the second protrusion 111 being insertable into the second recess 110 for enabling the upper end of the dialysis bag to be clamped between the fourth half of the third half 107. Between the third half 107 and the fourth half there is a locking bolt 115 for enabling the second protrusion 111 to remain inserted into the second recess 110. The two ends of the second fixing clamp can be respectively locked on the guide rod and the frame strip through fastening screws. It should be noted that the end of the third half and the fourth half that is connected to each other may be connected in a hinged manner, and the connected end is opposite to the end that is connected by the locking bolt. One end of the first half part and one end of the second half part which are connected with each other can be connected in a hinged mode; the connected end is opposite to the end connected by the locking bolt. The structure of the first fixing clip can be seen in the schematic view of the second fixing clip, i.e. as shown in fig. 5.
In an alternative to this embodiment, a guide rod 121 is also provided between the two spokes 103 of the connecting frame strip 104 in order to ensure stability of the dialysis bag during rotation of the dialysis turret. In order to apply the first fixing clip 105 and the second fixing clip 106 of different sizes, in this embodiment, the guide rod may also be slid along the axial direction of the spoke 103, and the end of the guide rod may be locked and fixed to the spoke 103 by the tightening screw 116.
In an alternative of this embodiment, the protein dialysis device further comprises a cover plate 117, the driving device is mounted on the cover plate 117, and the driving device and the dialysis turret are respectively located on two different plate surfaces of the cover plate 117. By providing the cover plate 117, it is convenient to mount the protein dialysis device at the opening of the container, for example, at the mouth of the beaker, and the cover plate 117 is supported by the mouth of the beaker.
In an alternative to this embodiment, the lower surface of the cover plate 117 is provided with a non-slip pad 118 for increasing the friction of the cover plate 117 with the opening of the container. In this embodiment, the protein dialysis device may further include a stabilizer, where the stabilizer includes a base and a vertical rod fixedly connected to the base; the base is provided with a stabilizing groove for placing the container, so that after the dialysis buffer solution is poured into the container, the container can be stabilized through the stabilizing groove.
In an alternative to this embodiment, the drive means is a motor 101.
In an alternative to this embodiment, the protein dialysis device further comprises a speed regulator 119 for regulating the rotational speed of the drive means and thus the rotational speed of the dialysis turret during rotation. The speed regulator 119 is mounted on the cover plate 117.
In an alternative to this embodiment, the protein dialysis device further comprises a battery 120 for providing power to the drive means. By using the battery 120, some of the active protein dialysis operations that need to be performed in a refrigerator at 4℃ can be accomplished. It should be noted that, the battery 120 may be a detachable battery 120, that is, a battery 120 bin for mounting the battery 120 is mounted on the cover 117.
In an alternative to this embodiment, the battery 120 is a rechargeable battery 120. The rechargeable battery 120 is a polymeric lithium ion rechargeable battery 120, a nickel chromium rechargeable battery 120, a nickel hydrogen rechargeable battery 120, a lithium polymer rechargeable battery 120, or a graphene rechargeable battery 120.
The specific applications of the protein dialysis device provided in this example are:
and (3) performing crude extraction on a cell to secrete and express active protein by using a protein dialysis device. The cell culture broth was centrifuged, the supernatant was taken, and the same volume of saturated ammonium sulfate solution (pH 7.0) was slowly added and stirred overnight. Centrifuge for 10min at 10000rpm, then discard supernatant and add PBS (pH 7.0) to suspend the pellet. The lower end of the sterilized dialysis bag is fixed with the first fixing clip 105 while the locking bolt 115 of the first fixing clip 105 is tightened to seal the lower end port of the dialysis bag. The prepared suspension is added from the upper end of the dialysis bag to a proper position by a pipette, the second fixing clamp 106 is slid to the upper end of the dialysis bag, and after clamping, the locking bolt 115 of the second fixing clamp 106 is screwed down, and the mouth is sealed. Lifting the whole protein dialysis device, immersing the dialysis rotating frame in PBS buffer solution, supporting the lower surface of the cover plate 117 by the beaker opening edge, moving the beaker and the protein dialysis device into a refrigerator at 4 ℃, and adjusting the speed regulator 119 to enable the dialysis rotating frame to reach a proper rotating speed to form vortex, thereby accelerating the precipitation of ammonium sulfate in the dialysis bag. When the solution is changed, the whole protein dialysis device is lifted, PBS buffer solution in a beaker is poured into a waste liquid cylinder, new PBS buffer solution is filled, and the dialysis bag is ensured to be below the liquid level of the PBS buffer solution. The liquid is changed every 2 to 3 hours until the ammonium sulfate is not detected in the PBS (Phosphate Buffer Saline) buffer solution. The runner is removed, the second fixing clamp 106 is opened, and the crude protein extract is sucked out by a pipette.
In summary, the protein dialysis apparatus is small in size, light in weight, and does not generate heat, and the cover plate 117 can be horizontally arranged on the edge of the beaker, and placed in a refrigerator at 4 ℃ together with the beaker to complete the dialysis work of active proteins.
Example two
The protein dialysis device in this embodiment is an improvement based on the first embodiment, and the technical content disclosed in the first embodiment is not repeated, and the disclosure of the first embodiment also belongs to the disclosure of the second embodiment.
In this embodiment, the protein dialysis device further comprises a drainage pump for draining the dialysis buffer in the container (e.g., beaker) and a fluid intake pump; the inlet pump is used to draw dialysis buffer into the container. This facilitates draining of the old dialysis buffer from the container and injecting the new dialysis buffer.
Example III
The protein dialysis system provided by the third embodiment of the invention comprises the protein dialysis device provided by the first embodiment or the second embodiment.
Finally, it should be noted that: the above embodiments are only for illustrating the technical solution of the present invention, and not for limiting the same; although the invention has been described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that: the technical scheme described in the foregoing embodiments can be modified or some or all of the technical features thereof can be replaced by equivalents; such modifications and substitutions do not depart from the spirit of the invention. Furthermore, those skilled in the art will appreciate that while some embodiments described herein include some features but not others included in other embodiments, combinations of features of different embodiments are meant to be within the scope of the invention and form different embodiments. For example, in the following claims, any of the claimed embodiments can be used in any combination.
Claims (10)
1. A protein dialysis device, which is characterized by comprising a driving device and a dialysis rotating frame; the driving device is used for driving the dialysis rotating frame to rotate; the dialysis rotating frame is used for bearing a dialysis bag, wherein the dialysis rotating frame comprises a wheel shaft and a wheel fan; the wheel fan is arranged on the wheel shaft through a spoke, the spoke is arranged along the radial direction of the wheel shaft, and the driving device drives the wheel shaft to rotate;
the number of the wheel fans is multiple, and the wheel fans are uniformly distributed along the circumference of the wheel shaft.
2. The protein dialysis apparatus of claim 1, wherein the dialysis turret is provided with a retaining clip assembly for clipping a dialysis bag.
3. The protein dialysis apparatus of claim 1, wherein the wheel fan comprises a rim strip; the frame strips extend along the axial direction of the wheel shaft; one end of the spoke is connected with the frame strip, and the other end of the spoke is connected with the wheel shaft.
4. A protein dialysis device according to any one of claims 1-3, further comprising a cover plate, said drive means being mounted on said cover plate, and said drive means and said dialysis turret being located on two different plate surfaces of said cover plate, respectively.
5. A protein dialysis device according to any one of claims 1-3, further comprising a speed regulator for regulating the rotational speed of the drive means.
6. A protein dialysis device according to any one of claims 1-3, further comprising a battery for providing power to the drive means.
7. The protein dialysis apparatus of claim 6, wherein the battery is a rechargeable battery.
8. The protein dialysis apparatus of claim 7, wherein the rechargeable battery is a polymeric lithium ion rechargeable battery, a nickel chromium rechargeable battery, a nickel hydrogen rechargeable battery, a lithium polymer rechargeable battery, or a graphene rechargeable battery.
9. A protein dialysis device according to any one of claims 1-3, wherein the drive means is an electric motor.
10. A protein dialysis system comprising a protein dialysis device according to any one of claims 1-9.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711403625.2A CN107857794B (en) | 2017-12-20 | 2017-12-20 | Protein dialysis device and system |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711403625.2A CN107857794B (en) | 2017-12-20 | 2017-12-20 | Protein dialysis device and system |
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| Publication Number | Publication Date |
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| CN107857794A CN107857794A (en) | 2018-03-30 |
| CN107857794B true CN107857794B (en) | 2024-04-02 |
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|---|---|---|---|
| CN201711403625.2A Active CN107857794B (en) | 2017-12-20 | 2017-12-20 | Protein dialysis device and system |
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Families Citing this family (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111659257A (en) * | 2020-06-05 | 2020-09-15 | 衡阳师范学院 | Dialysis device |
| CN114082321B (en) * | 2021-11-19 | 2023-06-27 | 陕西佰诺兮生物科技有限公司 | Continuous protein renaturation device based on enzyme engineering |
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|---|---|---|---|---|
| DE2737819A1 (en) * | 1977-08-22 | 1979-03-08 | Coca Cola Co | Electrodialysis with pairs of ultrafiltration membranes - and ions selective membrane at one end neutral membrane at other end |
| CN202415421U (en) * | 2011-12-29 | 2012-09-05 | 郑州伊美诺生物技术有限公司 | Simple dynamic protein dialysis system |
| CN102702309A (en) * | 2012-05-24 | 2012-10-03 | 张力 | Protein microdialyzer and usage method |
| CN204644239U (en) * | 2015-05-22 | 2015-09-16 | 青岛易邦生物工程有限公司 | Albumen dialysis apparatus |
| CN106574229A (en) * | 2014-08-22 | 2017-04-19 | 奥林巴斯株式会社 | Cell culture bag, cell culture device, and cell culture container |
| CN207793122U (en) * | 2017-12-20 | 2018-08-31 | 天康生物股份有限公司 | Protein dialysis apparatus and system |
-
2017
- 2017-12-20 CN CN201711403625.2A patent/CN107857794B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE2737819A1 (en) * | 1977-08-22 | 1979-03-08 | Coca Cola Co | Electrodialysis with pairs of ultrafiltration membranes - and ions selective membrane at one end neutral membrane at other end |
| CN202415421U (en) * | 2011-12-29 | 2012-09-05 | 郑州伊美诺生物技术有限公司 | Simple dynamic protein dialysis system |
| CN102702309A (en) * | 2012-05-24 | 2012-10-03 | 张力 | Protein microdialyzer and usage method |
| CN106574229A (en) * | 2014-08-22 | 2017-04-19 | 奥林巴斯株式会社 | Cell culture bag, cell culture device, and cell culture container |
| CN204644239U (en) * | 2015-05-22 | 2015-09-16 | 青岛易邦生物工程有限公司 | Albumen dialysis apparatus |
| CN207793122U (en) * | 2017-12-20 | 2018-08-31 | 天康生物股份有限公司 | Protein dialysis apparatus and system |
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| CN107857794A (en) | 2018-03-30 |
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