CN107857794A - Protein dialysis apparatus and system - Google Patents
Protein dialysis apparatus and system Download PDFInfo
- Publication number
- CN107857794A CN107857794A CN201711403625.2A CN201711403625A CN107857794A CN 107857794 A CN107857794 A CN 107857794A CN 201711403625 A CN201711403625 A CN 201711403625A CN 107857794 A CN107857794 A CN 107857794A
- Authority
- CN
- China
- Prior art keywords
- dialysis
- protein
- drive device
- rotating turret
- wheel
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
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Links
- 238000000502 dialysis Methods 0.000 title claims abstract description 81
- 102000004169 proteins and genes Human genes 0.000 title claims abstract description 51
- 108090000623 proteins and genes Proteins 0.000 title claims abstract description 51
- 238000004458 analytical method Methods 0.000 claims description 4
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 3
- PXHVJJICTQNCMI-UHFFFAOYSA-N Nickel Chemical compound [Ni] PXHVJJICTQNCMI-UHFFFAOYSA-N 0.000 claims description 3
- 229910018487 Ni—Cr Inorganic materials 0.000 claims description 3
- VNNRSPGTAMTISX-UHFFFAOYSA-N chromium nickel Chemical compound [Cr].[Ni] VNNRSPGTAMTISX-UHFFFAOYSA-N 0.000 claims description 3
- 229910021389 graphene Inorganic materials 0.000 claims description 3
- 229910001416 lithium ion Inorganic materials 0.000 claims description 3
- 229910000652 nickel hydride Inorganic materials 0.000 claims description 3
- 229920000642 polymer Polymers 0.000 claims description 3
- HBBGRARXTFLTSG-UHFFFAOYSA-N Lithium ion Chemical compound [Li+] HBBGRARXTFLTSG-UHFFFAOYSA-N 0.000 claims description 2
- 230000005611 electricity Effects 0.000 claims description 2
- 238000000465 moulding Methods 0.000 claims description 2
- 238000006116 polymerization reaction Methods 0.000 claims description 2
- 238000009738 saturating Methods 0.000 claims description 2
- 238000003760 magnetic stirring Methods 0.000 abstract description 6
- 239000012535 impurity Substances 0.000 abstract description 5
- 238000001742 protein purification Methods 0.000 abstract description 5
- 239000012149 elution buffer Substances 0.000 description 10
- 239000007788 liquid Substances 0.000 description 6
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 3
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 3
- 235000011130 ammonium sulphate Nutrition 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 238000007789 sealing Methods 0.000 description 3
- 239000000243 solution Substances 0.000 description 3
- 239000007853 buffer solution Substances 0.000 description 2
- 238000006073 displacement reaction Methods 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 239000006228 supernatant Substances 0.000 description 2
- 239000000725 suspension Substances 0.000 description 2
- 210000004885 white matter Anatomy 0.000 description 2
- 235000008331 Pinus X rigitaeda Nutrition 0.000 description 1
- 235000011613 Pinus brutia Nutrition 0.000 description 1
- 241000018646 Pinus brutia Species 0.000 description 1
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000000287 crude extract Substances 0.000 description 1
- 230000001934 delay Effects 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 239000008363 phosphate buffer Substances 0.000 description 1
- 238000005381 potential energy Methods 0.000 description 1
- 238000001556 precipitation Methods 0.000 description 1
- 230000000630 rising effect Effects 0.000 description 1
- 230000003248 secreting effect Effects 0.000 description 1
- 239000011780 sodium chloride Substances 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 230000006641 stabilisation Effects 0.000 description 1
- 238000011105 stabilization Methods 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 230000000007 visual effect Effects 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/34—Extraction; Separation; Purification by filtration, ultrafiltration or reverse osmosis
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Biochemistry (AREA)
- Analytical Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biophysics (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Water Supply & Treatment (AREA)
- External Artificial Organs (AREA)
Abstract
The present invention relates to Bioexperiment device technical field, more particularly, to a kind of protein dialysis apparatus and system.The protein dialysis apparatus, including drive device and dialysis rotating turret；Drive device is used to drive dialysis rotating turret to rotate；Dialysis rotating turret is used to carry bag filter, wherein, dialysis rotating turret includes wheel shaft and wheel is fanned；Wheel fan is installed on wheel shaft by spoke, and spoke being radially arranged along wheel shaft, and drive device drives wheel shaft to rotate.The protein dialysis system, including described protein dialysis apparatus.When can both avoid use magnetic stirring apparatus, sample leaks the present invention, is mutually wound between multiple bag filters, and the situation generation of damage occurs in bag filter, can accelerate appearing for bag filter small molecular albumen or other impurities again, reach the purpose of protein purification.
Description
Technical field
The present invention relates to Bioexperiment device technical field, more particularly, to a kind of protein dialysis apparatus and system.
Background technology
At present, it is that bag filter is put into elution buffer that protein dialysis is mostly, then actuates magnetic using magnetic stirring apparatus
Power rotor rotates, and is vortexed so that elution buffer is formed, so as to promote appearing for bag filter small molecular albumen or other impurities,
Retain purpose high molecular weight protein, reach the purpose of protein purification.Often occur one in use using magnetic stirring apparatus
A little problems, such as：With clip sealing or the bag filter with rope tying, magnetic rotor may mutually collide with sealing clip causes pine
Mouthful, cause sample to leak；During multiple sample dialysis, it may cause mutually to wind between bag filter, influence dialysis-effect；Magnetic rotor
Adjustment of rotational speed was not at that time, it is also possible to damages bag filter.
The content of the invention
It is an object of the invention to provide a kind of protein dialysis apparatus and system, to solve egg present in prior art
When white matter is dialysed, easily there is sample and leak, mutually wound between multiple bag filters, and bag filter occurs that the technology of damage is asked
Topic.
The invention provides a kind of protein dialysis apparatus, including drive device and dialysis rotating turret；The drive device
For driving the dialysis rotating turret to rotate；The dialysis rotating turret is used to carry bag filter, wherein, the dialysis rotating turret bag
Include wheel shaft and wheel is fanned；The wheel fan is installed on the wheel shaft by spoke, and the spoke being radially arranged along the wheel shaft,
The drive device drives the wheel shaft to rotate.
Further, fixed clip assembly is provided with the dialysis rotating turret, for clamping bag filter.
Further, the wheel fan includes frame bar；Axial direction extension of the frame bar along the wheel shaft；The wheel
One end of spoke is connected with the frame bar, the other end of the spoke and the wheel axis connection.
Further, in addition to cover plate, the drive device are installed on the cover plate, and the drive device with it is described
Rotating turret of dialysing is located at two different plate faces of the cover plate respectively.
Further, in addition to speed regulator, for adjusting the rotating speed of the drive device.
Further, in addition to battery, for providing power supply to the drive device.
Further, the battery is rechargeable battery.
Further, the rechargeable battery fills to polymerize Li-Ion rechargeable battery, nickel chromium triangle charging rechargeable battery, nickel hydride charging
Battery, lighium polymer rechargeable battery or graphene rechargeable battery.
Further, the drive device is motor.
Present invention also offers a kind of protein dialysis system, including described protein dialysis apparatus.
Compared with prior art, beneficial effects of the present invention are：
Protein dialysis apparatus provided by the invention, including drive device and dialysis rotating turret；Drive device is used to drive
Rotating turret of dialysing rotates；Dialysis rotating turret is used to carry bag filter, wherein, dialysis rotating turret includes wheel shaft and wheel is fanned；Wheel fan is logical
Cross spoke to be installed on wheel shaft, and spoke being radially arranged along wheel shaft, drive device drive wheel shaft to rotate.By the way that bag filter is pacified
Loaded on dialysis rotating turret on, and by drive device drive dialysis rotating turret rotate, just can so realize that plate bag also rotates,
So as to can also make elution buffer produce motion, when so can both avoid using magnetic stirring apparatus, sample leaks, Duo Getou
Analysis bag between mutually wind, and bag filter occur damage situation occur, can accelerate again bag filter small molecular albumen or
Other impurities appear, and reach the purpose of protein purification.
The protein dialysis system that the present invention also provides, including described protein dialysis apparatus.Can based on above-mentioned analysis
To know, when the protein dialysis system can both avoid using magnetic stirring apparatus, sample leaks, and is mutually wound between multiple bag filters,
And bag filter occurs that the situation of damage occurs, and can accelerate appearing for bag filter small molecular albumen or other impurities again,
Reach the purpose of protein purification.
Brief description of the drawings
, below will be to specific in order to illustrate more clearly of the specific embodiment of the invention or technical scheme of the prior art
The required accompanying drawing used is briefly described in embodiment or description of the prior art, it should be apparent that, in describing below
Accompanying drawing is some embodiments of the present invention, for those of ordinary skill in the art, before creative work is not paid
Put, other accompanying drawings can also be obtained according to these accompanying drawings.
Fig. 1 is the structural representation for the protein dialysis apparatus that the embodiment of the present invention one provides；
Fig. 2 is the close-up schematic view at A in Fig. 1；
Fig. 3 is the structural representation at another visual angle for the protein dialysis apparatus that the embodiment of the present invention one provides；
Fig. 4 is the close-up schematic view at B in Fig. 3；
Fig. 5 is the structural representation of the second geometrical clamp in the embodiment of the present invention one.
In figure：
101- motors；102- wheel shafts；103- spokes；104- frame bars；The geometrical clamps of 105- first；The geometrical clamps of 106- second；
The half portions of 107- the 3rd；The half portions of 108- the 4th；The grooves of 110- second；111- second is raised；115- clamping screws；116- tightens spiral shell
Nail；117- cover plates；118- slipmat；119- speed regulators；120- batteries；121- guide rods.
Embodiment
Technical scheme is clearly and completely described below in conjunction with accompanying drawing, it is clear that described implementation
Example is part of the embodiment of the present invention, rather than whole embodiments.Based on the embodiment in the present invention, ordinary skill
The every other embodiment that personnel are obtained under the premise of creative work is not made, belongs to the scope of protection of the invention.
In the description of the invention, it is necessary to explanation, term " " center ", " on ", " under ", "left", "right", " vertical ",
The orientation or position relationship of the instruction such as " level ", " interior ", " outer " be based on orientation shown in the drawings or position relationship, merely to
Be easy to the description present invention and simplify description, rather than instruction or imply signified device or element must have specific orientation,
With specific azimuth configuration and operation, therefore it is not considered as limiting the invention.In addition, term " first ", " second ",
" the 3rd " is only used for describing purpose, and it is not intended that instruction or hint relative importance.
In the description of the invention, it is necessary to illustrate, unless otherwise clearly defined and limited, term " installation ", " phase
Even ", " connection " should be interpreted broadly, for example, it may be being fixedly connected or being detachably connected, or be integrally connected；Can
To be mechanical connection or electrical connection；Can be joined directly together, can also be indirectly connected by intermediary, Ke Yishi
The connection of two element internals.For the ordinary skill in the art, with concrete condition above-mentioned term can be understood at this
Concrete meaning in invention.
Embodiment one
Referring to shown in Fig. 1 to Fig. 5, the embodiment of the present invention one provides a kind of protein dialysis apparatus, including drive device
With dialysis rotating turret；Drive device is used to drive dialysis rotating turret to rotate；Dialysis rotating turret is used to carry bag filter, wherein, thoroughly
Analysing rotating turret includes wheel shaft 102 and wheel fan；Wheel fan is installed on wheel shaft 102 by spoke 103, and spoke 103 is along wheel shaft 102
It is radially arranged, drive device drives wheel shaft 102 to rotate.
The protein dialysis apparatus that the embodiment provides, by the way that bag filter is installed on dialysis rotating turret, and pass through drive
Dynamic device drives dialysis rotating turret to rotate, and just can so realize that plate bag also rotates, so as to can also produce elution buffer
Raw motion；The protein dialysis apparatus that the embodiment provides has abandoned the side that magnetic stirring apparatus is stirred to elution buffer
Formula；And bag filter is fixed on dialysis rotating turret, and drive the dynamic dialysis rotating turret of drive device to rotate, it be able to can so subtract
The possibility that the sealing of sample bag filter loosens, so as to prevent sample device from leaking；Further, since bag filter is fixed on dialysis rotating turret
On, the situation that can so avoid multiple bag filters mutually to wind to a certain extent, but also bag filter can be avoided to damage
Bad situation occurs；Because bag filter can rotate, and elution buffer can also stir, so as to accelerate to dialyse
Bag small molecular albumen or other impurities appear, and reach the purpose of protein purification.
In the optional scheme of the embodiment, wheel fan includes frame bar 104；Axial direction of the frame bar 104 along wheel shaft 102 prolongs
Stretch, i.e., the length direction of frame bar 104 is parallel with the axial direction of wheel shaft 102；One end of spoke 103 is solid with frame bar 104
Fixed connection, the other end of spoke 103 are fixedly connected with wheel shaft 102.
Specifically, the quantity for taking turns fan is multiple, and multiple wheel fans are uniformly distributed along the circumference of wheel shaft 102, in order to wheel shaft
102 being capable of smooth rotation.The quantity of frame bar 104 in each wheel fan is two, and two frame bars 104 in each wheel fan
Axisymmetrical on wheel shaft 102.In the embodiment, exemplified by the quantity by wheel fan is two；Two wheel fans are in cross setting,
Central angle between two i.e. adjacent frame bars 104 is 90 °.
In the optional scheme of the embodiment, frame bar 104 passes through the spoke 103 that two parallel intervals are set and wheel shaft 102
It is fixedly connected, and two spokes 103 are setting up and down；The length direction of spoke 103 and the axis perpendicular of wheel shaft 102.
In the optional scheme of the embodiment, fixed clip assembly is provided with rotating turret of dialysing, for clamping bag filter.Each
A fixed clip assembly is provided with frame bar 104.
Specifically, fixed clip assembly bag the first geometrical clamp 105 and the second geometrical clamp 106, the second geometrical clamp 106 is positioned at the
The top of one geometrical clamp 105.
First geometrical clamp 105 includes the first half portion and the second half portion, and the first half portion is provided with the first groove, the second half portion tool
There is the first projection with the first fit depressions, the first projection is inserted into the first groove, for enabling the lower end of bag filter
Enough it is sandwiched between first the second half portion of half portion.There is clamping screw 115 between first half portion and the second half portion, it is first convex for making
The state of the first groove can be remained inserted into by rising.First geometrical clamp 105 is fixed on underlying spoke 103.Second fixes
Folder 106 can slide along the length direction of frame bar 104.
Second geometrical clamp 106 includes the 3rd half portion 107 and the 4th half portion 108, and the 3rd half portion 107 is provided with the second groove
110, the 4th half portion has the second projection 111 being engaged with the second groove 110, and the second projection 111 is inserted into the second groove
In 110, the upper end for enabling bag filter is sandwiched between the half portion of the 3rd half portion 107 the 4th.3rd half portion 107 and the 4th half portion
Between there is clamping screw 115, for enabling the second projection 111 to remain inserted into the state of the second groove 110.Second geometrical clamp
Both ends can be lock onto respectively on guide rod and frame bar by trip bolt.It should be noted that the 3rd half portion and the 4th half
The one end being connected between portion can be connected by way of be hinged, what the one end being connected was connected with by clamping screw
One end is relative.The one end being connected between first half portion and the second half portion can be connected by way of be hinged；What this was connected
One end is relative with the one end being connected by clamping screw.The structure of first geometrical clamp may refer to the signal of the second geometrical clamp
Figure, i.e., as shown in Figure 5.
In the optional scheme of the embodiment, in order to ensure in rotating turret rotation process of dialysing, the stability of bag filter, even
Guide rod 121 is also set up between two spokes 103 of edge fit moulding 104.It should be noted that it is different size of in order to be applicable,
First geometrical clamp 105 and the second geometrical clamp 106, it in the embodiment, can also slide axial direction of the guide rod along spoke 103
It is dynamic, and the end of guide rod is locked by tightening screw 116 with spoke 103.
In the optional scheme of the embodiment, protein dialysis apparatus also includes cover plate 117, and drive device is installed on cover plate
On 117, and drive device is located at two different plate faces of cover plate 117 from dialysis rotating turret respectively.By setting cover plate 117,
Be easy to protein dialysis apparatus being installed on the opening of container, be such as installed at the rim of a cup of beaker, cover plate 117 by beaker cup
Mouthful support.
In the embodiment in optional scheme, the lower surface of cover plate 117 is provided with slipmat 118, for increasing cover plate 117
With the opening frictional force of container.It should be noted that in the embodiment, protein dialysis apparatus can also include steady rest, surely
Determining frame includes base and the vertical rod being fixedly connected with base；Gutter position is provided with base, so will be saturating for placing container
After analysis buffer solution pours into container, the stabilization of container is enough realized by gutter potential energy.
In the optional scheme of the embodiment, drive device is motor 101.
In the optional scheme of the embodiment, protein dialysis apparatus also includes speed regulator 119, for adjusting driving dress
The rotating speed put, so as to adjust rotating speed of the dialysis rotating turret in rotation process.Speed regulator 119 is installed on cover plate 117.
In the optional scheme of the embodiment, protein dialysis apparatus also includes battery 120, for providing electricity to drive device
Source.By using battery 120, it is possible to achieve the operation that the dialysis of some reactive proteins need to be completed in 4 DEG C of refrigerators.Need to illustrate
, battery 120 can be detachable battery 120, i.e., the storehouse of battery 120 for installing battery 120 is provided with cover plate 117.
In the optional scheme of the embodiment, battery 120 is rechargeable battery 120.The rechargeable battery 120 fills for polymerization lithium ion
Battery 120, nickel chromium triangle charging rechargeable battery 120, nickel hydride charging rechargeable battery 120, lighium polymer rechargeable battery 120 or graphene
Rechargeable battery 120.
The protein dialysis apparatus that the embodiment provides has particular application as：
Certain cell secreting, expressing activated protein is slightly carried using protein dialysis apparatus.Centrifuge cell nutrient solution, takes supernatant, delays
It is slow to add the full conjunction ammonium sulfate (PH7.0) of same volume, it is stirred overnight.By centrifuge 10min, rotating speed 10000rpm,
Then supernatant is abandoned, and adds PBS (PH7.0) suspension precipitations.The lower end of bag filter after sterilization is consolidated with the first geometrical clamp 105
It is fixed, while the clamping screw 115 of the first geometrical clamp 105 is tightened, the lower end port of bag filter is sealed.From the upper of bag filter
End suspension made from pipettor adds slides the second geometrical clamp 106 to the upper end of bag filter to appropriate location, after jaws,
The clamping screw 115 of second geometrical clamp 106 is tightened, seals mouth.Lift whole protein dialysis apparatus device, dialysis is rotated
Frame is immersed in PBS, and the lower surface of cover plate 117 is supported by beaker opening's edge, by beaker and protein dialysis apparatus together
Move into 4 DEG C of refrigerators, governing speed adjuster 119, dialysis rotating turret is formed and is vortexed, so as to accelerate to dialyse to suitable rotational speed
Ammonium sulfate separates out in bag.When changing liquid, whole protein dialysis apparatus is lifted, PBS in beaker is poured into waste liquid cylinder, added
Note new PBS, it is ensured that bag filter is below the liquid level of PBS.Liquid is changed per 2h~3h once, until PBS
Untill ammonium sulfate being can't detect in (Phosphate Buffer Saline) buffer solution.Runner is removed, opens the second geometrical clamp
106, suction out protein crude extract with pipettor.
In summary, protein dialysis apparatus small volume, in light weight, the not heat production, cover plate 117 can crossbearer in beaker edge
On, it is put into together with beaker in 4 DEG C of refrigerators, completes the dialysis work of activated protein.
Embodiment two
Protein dialysis apparatus in the present embodiment is the improvement on the basis of embodiment one, the skill disclosed in embodiment one
Art content not repeated description, the disclosure of embodiment one fall within the disclosure of the present embodiment two.
In the embodiment, protein dialysis apparatus also includes positive displacement pump and liquid feeding pump, and positive displacement pump is used for container (as burnt
Cup) in elution buffer discharge；Liquid feeding pump is used to elution buffer being pumped into container.So being easy to will be old in container
Elution buffer is discharged, and injects new elution buffer.
Embodiment three
A kind of protein dialysis system that the embodiment of the present invention three provides includes the egg that embodiment one or embodiment two provide
White matter dialysis apparatus.
Finally it should be noted that：Various embodiments above is merely illustrative of the technical solution of the present invention, rather than its limitations；To the greatest extent
The present invention is described in detail with reference to foregoing embodiments for pipe, it will be understood by those within the art that：Its according to
The technical scheme described in foregoing embodiments can so be modified, either which part or all technical characteristic are entered
Row equivalent substitution；And these modifications or replacement, the essence of appropriate technical solution is departed from various embodiments of the present invention technology
The scope of scheme.In addition, it will be appreciated by those of skill in the art that although some embodiments described herein include other implementations
Included some features rather than further feature in example, but the combination of the feature of different embodiments means in the present invention
Within the scope of and form different embodiments.For example, in the following claims, embodiment claimed
It is one of any mode to use in any combination.
Claims (10)
1. a kind of protein dialysis apparatus, it is characterised in that including drive device and dialysis rotating turret；The drive device is used for
The dialysis rotating turret is driven to rotate；The dialysis rotating turret is used to carry bag filter, wherein, the dialysis rotating turret includes wheel
Axle and wheel are fanned；The wheel fan is installed on the wheel shaft by spoke, and the spoke being radially arranged along the wheel shaft, described
Drive device drives the wheel shaft to rotate.
2. protein dialysis apparatus according to claim 1, it is characterised in that be provided with fixation on the dialysis rotating turret
Clip assembly, for clamping bag filter.
3. protein dialysis apparatus according to claim 1, it is characterised in that the wheel fan includes frame bar；The side
Axial direction extension of the moulding along the wheel shaft；One end of the spoke is connected with the frame bar, the other end of the spoke
With the wheel axis connection.
4. the protein dialysis apparatus according to any one of claim 1-3, it is characterised in that described also including cover plate
Drive device is installed on the cover plate, and the drive device is located at two of the cover plate with the dialysis rotating turret respectively
Different plate faces.
5. the protein dialysis apparatus according to any one of claim 1-3, it is characterised in that also adjusted including speed
Device, for adjusting the rotating speed of the drive device.
6. the protein dialysis apparatus according to any one of claim 1-3, it is characterised in that also including battery, be used for
Power supply is provided to the drive device.
7. protein dialysis apparatus according to claim 6, it is characterised in that the battery is rechargeable battery.
8. protein dialysis apparatus according to claim 7, it is characterised in that the rechargeable battery fills for polymerization lithium ion
Battery, nickel chromium triangle charging rechargeable battery, nickel hydride charging rechargeable battery, lighium polymer rechargeable battery or graphene rechargeable battery.
9. the protein dialysis apparatus according to any one of claim 1-3, it is characterised in that the drive device is electricity
Machine.
10. a kind of protein dialysis system, it is characterised in that saturating including protein as claimed in any one of claims 1-9 wherein
Analysis apparatus.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711403625.2A CN107857794B (en) | 2017-12-20 | 2017-12-20 | Protein dialysis device and system |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711403625.2A CN107857794B (en) | 2017-12-20 | 2017-12-20 | Protein dialysis device and system |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN107857794A true CN107857794A (en) | 2018-03-30 |
| CN107857794B CN107857794B (en) | 2024-04-02 |
Family
ID=61706921
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201711403625.2A Active CN107857794B (en) | 2017-12-20 | 2017-12-20 | Protein dialysis device and system |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN107857794B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111659257A (en) * | 2020-06-05 | 2020-09-15 | 衡阳师范学院 | Dialysis device |
| CN114082321A (en) * | 2021-11-19 | 2022-02-25 | 邓林峰 | Continuous type protein renaturation device based on enzyme engineering |
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| DE2737819A1 (en) * | 1977-08-22 | 1979-03-08 | Coca Cola Co | Electrodialysis with pairs of ultrafiltration membranes - and ions selective membrane at one end neutral membrane at other end |
| CN202415421U (en) * | 2011-12-29 | 2012-09-05 | 郑州伊美诺生物技术有限公司 | Simple dynamic protein dialysis system |
| CN102702309A (en) * | 2012-05-24 | 2012-10-03 | 张力 | Protein microdialyzer and usage method |
| CN204644239U (en) * | 2015-05-22 | 2015-09-16 | 青岛易邦生物工程有限公司 | Albumen dialysis apparatus |
| CN106574229A (en) * | 2014-08-22 | 2017-04-19 | 奥林巴斯株式会社 | Cell culture bag, cell culture device, and cell culture container |
| CN207793122U (en) * | 2017-12-20 | 2018-08-31 | 天康生物股份有限公司 | Protein dialysis apparatus and system |
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2017
- 2017-12-20 CN CN201711403625.2A patent/CN107857794B/en active Active
Patent Citations (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE2737819A1 (en) * | 1977-08-22 | 1979-03-08 | Coca Cola Co | Electrodialysis with pairs of ultrafiltration membranes - and ions selective membrane at one end neutral membrane at other end |
| CN202415421U (en) * | 2011-12-29 | 2012-09-05 | 郑州伊美诺生物技术有限公司 | Simple dynamic protein dialysis system |
| CN102702309A (en) * | 2012-05-24 | 2012-10-03 | 张力 | Protein microdialyzer and usage method |
| CN106574229A (en) * | 2014-08-22 | 2017-04-19 | 奥林巴斯株式会社 | Cell culture bag, cell culture device, and cell culture container |
| CN204644239U (en) * | 2015-05-22 | 2015-09-16 | 青岛易邦生物工程有限公司 | Albumen dialysis apparatus |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111659257A (en) * | 2020-06-05 | 2020-09-15 | 衡阳师范学院 | Dialysis device |
| CN114082321A (en) * | 2021-11-19 | 2022-02-25 | 邓林峰 | Continuous type protein renaturation device based on enzyme engineering |
Also Published As
| Publication number | Publication date |
|---|---|
| CN107857794B (en) | 2024-04-02 |
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