CN107841483A - 一种提取动物组织线粒体的方法 - Google Patents
一种提取动物组织线粒体的方法 Download PDFInfo
- Publication number
- CN107841483A CN107841483A CN201711115107.0A CN201711115107A CN107841483A CN 107841483 A CN107841483 A CN 107841483A CN 201711115107 A CN201711115107 A CN 201711115107A CN 107841483 A CN107841483 A CN 107841483A
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- China
- Prior art keywords
- mitochondria
- animal tissue
- thin slice
- suspension
- tissue
- Prior art date
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0603—Embryonic cells ; Embryoid bodies
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
- C12N5/06—Animal cells or tissues; Human cells or tissues
- C12N5/0602—Vertebrate cells
- C12N5/0603—Embryonic cells ; Embryoid bodies
- C12N5/0605—Cells from extra-embryonic tissues, e.g. placenta, amnion, yolk sac, Wharton's jelly
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2509/00—Methods for the dissociation of cells, e.g. specific use of enzymes
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- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Genetics & Genomics (AREA)
- Wood Science & Technology (AREA)
- Gynecology & Obstetrics (AREA)
- Reproductive Health (AREA)
- Developmental Biology & Embryology (AREA)
- Cell Biology (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Pregnancy & Childbirth (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
Description
Claims (8)
Priority Applications (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201711115107.0A CN107841483B (zh) | 2017-11-13 | 2017-11-13 | 一种提取动物组织线粒体的方法 |
PCT/CN2018/079790 WO2019091034A1 (zh) | 2017-11-13 | 2018-03-21 | 一种提取动物组织线粒体的方法 |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
CN201711115107.0A CN107841483B (zh) | 2017-11-13 | 2017-11-13 | 一种提取动物组织线粒体的方法 |
Publications (2)
Publication Number | Publication Date |
---|---|
CN107841483A true CN107841483A (zh) | 2018-03-27 |
CN107841483B CN107841483B (zh) | 2021-08-03 |
Family
ID=61681573
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
CN201711115107.0A Active CN107841483B (zh) | 2017-11-13 | 2017-11-13 | 一种提取动物组织线粒体的方法 |
Country Status (2)
Country | Link |
---|---|
CN (1) | CN107841483B (zh) |
WO (1) | WO2019091034A1 (zh) |
Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101717772A (zh) * | 2009-12-18 | 2010-06-02 | 聊城大学 | 一种动物线粒体dna的提取及纯化方法 |
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2017
- 2017-11-13 CN CN201711115107.0A patent/CN107841483B/zh active Active
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2018
- 2018-03-21 WO PCT/CN2018/079790 patent/WO2019091034A1/zh active Application Filing
Patent Citations (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN101717772A (zh) * | 2009-12-18 | 2010-06-02 | 聊城大学 | 一种动物线粒体dna的提取及纯化方法 |
Non-Patent Citations (3)
Title |
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DEVALL M 等: "A comparison of mitochondrial DNA isolation methods in frozen post-mortem human brain tissue--applications for studies of mitochondrial genetics in brain disorders", 《BIOTECHNIQUES》 * |
夏玉玲 等: "动物线粒体DNA提取的原理和方法", 《蚕学通讯》 * |
臧莹安 等: "从动物组织中提取线粒体的方法", 《中国兽医杂志》 * |
Also Published As
Publication number | Publication date |
---|---|
WO2019091034A1 (zh) | 2019-05-16 |
CN107841483B (zh) | 2021-08-03 |
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Legal Events
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PB01 | Publication | ||
PB01 | Publication | ||
SE01 | Entry into force of request for substantive examination | ||
SE01 | Entry into force of request for substantive examination | ||
TA01 | Transfer of patent application right | ||
TA01 | Transfer of patent application right |
Effective date of registration: 20210701 Address after: 519000 Room 204, No.10, jingle Road, Jida, Xiangzhou District, Zhuhai City, Guangdong Province Applicant after: Zhuhai lingvalue Biotechnology Co.,Ltd. Address before: 510000 unit 1001, 10 / F, building F, Gaode land autumn block, 11 Zhujiang East Road, Tianhe District, Guangzhou City, Guangdong Province Applicant before: GUANGZHOU ZISHENG BIOTECHNOLOGY Co.,Ltd. |
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GR01 | Patent grant | ||
GR01 | Patent grant | ||
CP03 | Change of name, title or address | ||
CP03 | Change of name, title or address |
Address after: Room 1602, 1603, 1604, 16th Floor, Building 1, Sanshan Science and Technology Innovation Center, No. 12 Ganggang Road, Guicheng Street, Nanhai District, Foshan City, Guangdong Province, 528000 Patentee after: Guangdong Age Value Biotechnology Co.,Ltd. Address before: 519000 Room 204, No.10, jingle Road, Jida, Xiangzhou District, Zhuhai City, Guangdong Province Patentee before: Zhuhai lingvalue Biotechnology Co.,Ltd. |