CN107828634A - A kind of ultrasonic fermentation reactor - Google Patents
A kind of ultrasonic fermentation reactor Download PDFInfo
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- CN107828634A CN107828634A CN201711361612.3A CN201711361612A CN107828634A CN 107828634 A CN107828634 A CN 107828634A CN 201711361612 A CN201711361612 A CN 201711361612A CN 107828634 A CN107828634 A CN 107828634A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M27/00—Means for mixing, agitating or circulating fluids in the vessel
- C12M27/02—Stirrer or mobile mixing elements
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
- C12M35/02—Electrical or electromagnetic means, e.g. for electroporation or for cell fusion
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
- C12M35/04—Mechanical means, e.g. sonic waves, stretching forces, pressure or shear stimuli
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
- C12M41/22—Heat exchange systems, e.g. heat jackets or outer envelopes in contact with the bioreactor walls
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
- C12M41/12—Means for regulation, monitoring, measurement or control, e.g. flow regulation of temperature
- C12M41/18—Heat exchange systems, e.g. heat jackets or outer envelopes
- C12M41/24—Heat exchange systems, e.g. heat jackets or outer envelopes inside the vessel
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
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Abstract
The invention provides a kind of ultrasonic fermentation reactor.The ultrasonic fermentation reactor includes tank body, supersonic generator and electromagnetic pulse reactor array.Tank body is provided with material inlet, material outlet, gas vent and water body outlet, material inlet will treat that fermentation materials are delivered in tank body, material after fermentation is discharged tank body by material outlet, caused gas during material fermentation is discharged tank body by gas vent, and caused water during material fermentation is discharged tank body by water body outlet;Supersonic generator is arranged on outside tank body or in tank body, to produce ultrasonic wave into tank body during material fermentation;Electromagnetic pulse reactor array is formed and is arranged in tank body by multiple electromagnetic pulse reactors, to produce electromagnetic pulse during material fermentation.The present invention can improve the quality and efficiency of fermentation, shorten fermentation time, and applied widely;The fermentation process of farming and animal husbandry pollution (excrement of animals, stalk), and the effect with organic energy-conserving and environment-protective can be applied to.
Description
Technical field
The present invention relates to installation for fermenting technical field, it relates in particular to which one kind can be used in making bio-bacterial manure, fermentation
The ultrasonic fermentation reactor of food, fermented beverage etc..
Background technology
At present, fermentation technique has obtained tremendous development, and its scope covers food fermentation (such as Yoghourt, cheese, bread, sauce
Salted vegetables, fermented soya bean, fermented bean curd, fermentation flesh of fish etc.), brewage (such as beer, white wine, yellow rice wine, grape wine alcoholic drink and soy sauce, sauce, vinegar
Deng brewing seasonings etc.), the fermentation industry (such as alcohol, lactic acid, acetone, butanol) in modern age etc..
However, there is the deficiencies of fermentation time is long, production efficiency is not high enough in existing fermentation technique.
The content of the invention
It is an object of the invention to solve at least one in deficiencies of the prior art.For example, the present invention
The first purpose is to provide a kind of installation for fermenting that can effectively improve fermentation efficiency.
To achieve these goals, the invention provides a kind of ultrasonic fermentation reactor.The ultrasonic fermentation reaction
Kettle includes tank body, supersonic generator and electromagnetic pulse reactor array, wherein, the tank body is provided with material inlet, material
Outlet, gas vent and water body outlet, the material inlet can will treat that fermentation materials are delivered in tank body, the material outlet
Material after fermentation can be discharged tank body, the gas vent can be by caused gas drain tank during material fermentation
Caused water during material fermentation can be discharged tank body by body, the water body outlet;The supersonic generator is arranged on tank
In external or tank body, to produce ultrasonic wave into the tank body during material fermentation;The electromagnetic pulse reactor array
Formed and be arranged in tank body by multiple electromagnetic pulse reactors, to produce electricity in the tank interior during material fermentation
Magnetic field impulse.
According to one exemplary embodiment of the present invention, the ultrasonic fermentation reactor may also include mixing component, institute
Stating mixing component can be stirred to the material in ultrasonic fermentation reactor.For example, the mixing component may include to set
The deceleration of percussion mechanism, the motor and the connection percussion mechanism and the motor that are arranged on outside tank body in tank body
Mechanism.In addition, the percussion mechanism may include to be vertically disposed at vibrations main shaft in tank body and it is one or more with
The vibrations arm of main shaft interconnection.In addition, the multiple electromagnetic pulse reactor can be uniformly arranged on one or more of shakes
On swing arm.
According to one exemplary embodiment of the present invention, the ultrasonic fermentation reactor may also include temperature-controlling component, institute
Fermentation temperature can be controlled by stating temperature-controlling component.For example, the temperature-controlling component may include temperature adjusting member, feedback computing unit and
One or more temperature sensors, wherein, the temperature adjusting member is arranged on outside tank interior or tank body, and can be to tank
Internal material is heated or cooled;One or more of temperature sensors are arranged in tank body and being capable of real-time detectable substances
Material temperature degree;The temperature value and biofermentation target that the feedback computing unit detects to obtain according to one or more of temperature sensors
The comparison of temperature is heated or cooled adjusting the temperature adjusting member.
According to one exemplary embodiment of the present invention, the ultrasonic fermentation reactor may also include tank body control climate
Unit, the tank body control climate unit can control the atmosphere in the tank body to form.
According to one exemplary embodiment of the present invention, the material inlet may be provided at the top of the tank body, described
Material outlet may be provided at the bottom of the tank body, and the gas vent may be provided at the top of the tank body, and the water body goes out
Mouth may be provided at the bottom of the tank body.
According to one exemplary embodiment of the present invention, the ultrasonic fermentation reactor, which may also include, is arranged on the tank
Electromagnetic pulse insulation blocking cover outside body.
Compared with prior art, beneficial effects of the present invention include:The efficiency of fermentation can be improved, shortens fermentation time.
In addition, the present invention can also improve fermented quality, and it is applied widely.
Brief description of the drawings
Fig. 1 shows the structural representation of an exemplary embodiment of the ultrasonic fermentation reactor according to the present invention.
Fig. 2 shows the structural representation of another exemplary embodiment of the ultrasonic fermentation reactor according to the present invention
Figure.
Embodiment
Hereinafter, exemplary embodiment will be combined to describe the ultrasonic fermentation reactor of the present invention in detail.
Fig. 1 shows the structural representation of an exemplary embodiment of the ultrasonic fermentation reactor according to the present invention.
As shown in figure 1, in one exemplary embodiment of the present invention, ultrasonic fermentation reactor may include tank body, surpass
Sonic generator and electromagnetic pulse reactor array.
Wherein, tank body 10 is provided with material inlet 10A, material outlet 10B, gas vent 10C and water body outlet 10D.Such as
Shown in Fig. 1, material inlet may be provided at the top (for example, upper left quarter) of tank body, and it can will treat that fermentation materials are delivered to tank body
It is interior.Material outlet may be provided at the bottom (for example, right lower quadrant) of tank body, and the material after fermentation can be discharged tank body by it.Gas
Outlet may be provided at the top (for example, top to the right position) of tank body, and it can be by caused gas during material fermentation
Discharge tank body.Water body outlet may be provided at the bottom (for example, bottom to the left position) of tank body, and it can be by material fermentation process
In caused water (alternatively referred to as pending water or waste water) discharge tank body.
Supersonic generator 12 may be provided at the top of tank body, be sent with the material during material fermentation into tank body
Ultrasonic wave.The frequency for the ultrasonic wave that supersonic generator is sent can be 19~60kHz, and power can be 15~30W.Ultrasonic wave
The frequency for the ultrasonic wave that generator is sent can also be 30~45kHz, and power can be 22~28W.However, the invention is not restricted to
This, supersonic generator may also be arranged on sidepiece or the bottom of the outer wall of tank body, may also be arranged on the inside of tank body.Electromagnetic pulse
Reactor array can be formed and be arranged in tank body by multiple electromagnetic pulse reactors 11, with during material fermentation in institute
State tank interior and produce electromagnetic pulse.As shown in figure 1, electromagnetic pulse reactor array can be evenly arranged in tank body by 9
Electromagnetic pulse reactor is formed.Supersonic generator ultrasonic wave and electromagnetic pulse reactor array electromagnetic pulse work
Under, the fermentation process of material can be greatly speeded up, and so as to be advantageous to improve fermentation efficiency, shorten fermentation time.For example, electromagnetism
Pulse can be microwave pulse.
In another exemplary embodiment of the present invention, what ultrasonic fermentation reactor can be as described in Figure 1 is exemplary
Further comprise temperature-controlling component on the basis of embodiment.Wherein, temperature-controlling component can control the temperature in fermentation cauldron, so that fermentation
Temperature in kettle meets requirement of a variety of zymotechniques to fermentation temperature.
For example, temperature-controlling component may include temperature adjusting member, feedback computing unit and temperature sensor.Wherein, temperature is adjusted
Section component is arranged on outside tank interior or tank body, and the material in tank body can be heated or cooled.For example, temperature is adjusted
It can be by ring-type water-filled pipe to save component, and can be realized by controlling the temperature of ring-type water-filled pipe reclaimed water to material
Heated or cooled.However, the invention is not restricted to this, for example, can also pass through the component with heating unit and temperature reducing unit
To form temperature adjusting member.Temperature sensor may be provided in tank body or outside tank body, and can detect temperature of charge in real time.Temperature
The quantity for spending sensor can be one or more.Feedback computing unit can detect to obtain according to one or more temperature sensors
The comparison of temperature value and biofermentation target temperature heated or cooled adjusting temperature adjusting member so that tank body temperature expires
Requirement of a variety of zymotechniques of foot to fermentation temperature.
In addition, in one exemplary embodiment of the present invention, ultrasonic fermentation reactor may also include tank body atmosphere control
Unit processed.Tank body control climate unit can control the atmosphere in tank body to form so that the atmosphere in fermentation cauldron meet it is various not
Requirement of the same zymotechnique to atmosphere of fermenting.For example, it is to detest that can control atmosphere in tank body by tank body control climate unit
Oxygen atmosphere has oxygen atmosphere.
By temperature-controlling component and tank body control climate unit, sent out according to the ultrasonic wave of one exemplary embodiment of the present invention
Ferment reactor can control temperature and atmosphere in fermentation cauldron, so that the temperature and atmosphere in fermentation cauldron meet a variety of hairs
Ferment (for example, Yoghourt, cheese, bread, catsup and pickled vegetables, fermented soya bean, fermented bean curd, fermentation flesh of fish etc. food fermentation, such as beer, white wine,
The brewageing of the brewing seasonings such as the alcoholic drinks such as yellow rice wine, grape wine and soy sauce, sauce, vinegar etc., the fermentation of Chinese herbal medicine is drawn and all
Such as excrement of animals, various stalks agriculturally, feed, the fermentation of life swill rubbish synthesis) technique is to fermentation temperature and atmosphere
Requirement.
In addition, in one exemplary embodiment of the present invention, ultrasonic fermentation reactor, which may also include, is arranged on tank body
Outside electromagnetic pulse insulation blocking cover, to avoid influence of the too strong electromagnetic pulse to non-fermented material.
Fig. 2 shows the structural representation of another exemplary embodiment of the ultrasonic fermentation reactor according to the present invention
Figure.
As shown in Fig. 2 in another exemplary embodiment of the present invention, ultrasonic fermentation reactor may include tank body
10th, supersonic generator 12, electromagnetic pulse reactor array, mixing component and temperature-controlling component.
Wherein, tank body 10 is provided with material inlet 10A, material outlet 10B, gas vent 10C and water body outlet 10D.Such as
Shown in Fig. 2, material inlet may be provided at the top (for example, left top side portion) of tank body, and it can will treat that fermentation materials are delivered to tank
In vivo.Material outlet may be provided at the bottom (for example, right lower quadrant) of tank body, and the material after fermentation can be discharged tank body by it.Gas
Body outlet may be provided at the top (for example, top to the left position) of tank body, and it can be by caused gas during material fermentation
Body discharges tank body.Water body outlet may be provided at the bottom (for example, bottom to the left position) of tank body, and it can be by material fermentation mistake
Caused water (alternatively referred to as pending water or waste water) discharge tank body in journey.
Supersonic generator 12 may be provided at the top of tank body, be sent with the material during material fermentation into tank body
Ultrasonic wave.The frequency for the ultrasonic wave that supersonic generator is sent can be 19~60kHz, and power can be 15~30W.Ultrasonic wave
The frequency for the ultrasonic wave that generator is sent can also be 30~45kHz, and power can be 22~28W.However, the invention is not restricted to
This, supersonic generator may also be arranged on sidepiece or the bottom of the outer wall of tank body, may also be arranged on the inside of tank body.
Electromagnetic pulse reactor array can be formed and be arranged in tank body by multiple electromagnetic pulse reactors, with material
In fermentation process electromagnetic pulse is produced in the tank interior.As shown in Fig. 2 electromagnetic pulse reactor array can be by 6 uniformly
The electromagnetic pulse reactor 11 being arranged in tank body is formed.In the ultrasonic wave and electromagnetic pulse reactor battle array of supersonic generator
In the presence of the electromagnetic pulse of row, the fermentation process of material can be greatly speeded up, and so as to be advantageous to improve fermentation efficiency, shorten hair
The ferment time.For example, electromagnetic pulse can be microwave pulse.
Mixing component can be stirred to the material in ultrasonic fermentation reactor.As shown in Fig. 2 mixing component can wrap
The motor 15A and the connection percussion mechanism that include the percussion mechanism being arranged in tank body, are arranged on outside tank body and the electricity
The reducing gear 15B of machine.For example, percussion mechanism may include to be vertically disposed at the vibrations main shaft 14A and one in tank body
Individual or multiple and main shaft interconnection vibrations arm 14B.In addition, the multiple electromagnetic pulse reactors for forming electromagnetic pulse array can
It is uniformly arranged on one or more of vibrations arms.Material can be stirred during the fermentation by mixing component,
Be advantageous to improve fermented quality and efficiency, and shorten fermentation time.
Temperature-controlling component can control the temperature in fermentation cauldron, so that the temperature in fermentation cauldron meets a variety of fermentation works
Requirement of the skill to fermentation temperature.For example, temperature-controlling component may include temperature adjusting member (not shown in Fig. 2), feedback computing unit
(not shown in Fig. 2) and temperature sensor 13.Wherein, temperature adjusting member is arranged on outside tank interior or tank body, and can
Material in tank body is heated or cooled.For example, temperature adjusting member can be by ring-type water-filled pipe, and can lead to
The temperature for crossing control ring-type water-filled pipe reclaimed water is heated or cooled to material to realize.However, the invention is not restricted to this, example
Such as, also temperature adjusting member can be formed by the component with heating unit and temperature reducing unit.Temperature sensor may be provided at
In tank body or outside tank body, and temperature of charge can be detected in real time.The quantity of temperature sensor can be one or more.Feedback meter
The comparison of the unit temperature value that can detect to obtain according to one or more temperature sensors and biofermentation target temperature is calculated to adjust temperature
Degree adjustment means are heated or cooled, so that tank body temperature meets that a variety of zymotechniques are wanted to fermentation temperature
Ask.
In another exemplary embodiment of the present invention, what ultrasonic fermentation reactor can be as described in Figure 2 is exemplary
Further comprise tank body control climate unit on the basis of embodiment.Tank body control climate unit can control the atmosphere in tank body
Form, so that the atmosphere in fermentation cauldron meets requirement of a variety of zymotechniques to atmosphere of fermenting.For example, pass through tank body gas
Atmosphere control unit can control atmosphere in tank body to be anaerobism atmosphere or have oxygen atmosphere.
By temperature-controlling component and tank body control climate unit, sent out according to the ultrasonic wave of one exemplary embodiment of the present invention
Ferment reactor can control temperature and atmosphere in fermentation cauldron, so that the temperature and atmosphere in fermentation cauldron meet a variety of hairs
Ferment (for example, Yoghourt, cheese, bread, catsup and pickled vegetables, fermented soya bean, fermented bean curd, fermentation flesh of fish etc. food fermentation, such as beer, white wine,
The brewageing of the brewing seasonings such as the alcoholic drinks such as yellow rice wine, grape wine and soy sauce, sauce, vinegar etc., the fermentation of Chinese herbal medicine is drawn and all
Such as excrement of animals, various stalks agriculturally, feed, the fermentation of life swill rubbish synthesis) technique is to fermentation temperature and atmosphere
Requirement.
For example, such as excrement of animals, agriculture are handled by the ultrasonic fermentation reactor of the exemplary embodiment of the present invention
Various stalks, life swill in industry etc., the bio-bacterial manure absorbed can be obtained efficiently and be easy to, and can effectively handle each
Class discarded object, turns waste into wealth, and can avoid the pollution and destruction of livestock sewage, excrement, life swill to environment, and ferments
Efficiency is greatly improved.Food fermentation or medium-height grass are carried out by the ultrasonic fermentation reactor of the exemplary embodiment of the present invention
The fermentation of medicine is drawn, it is possible to increase fermented quality, and fermentation efficiency is greatly improved.That is, the present invention can be applicable
In food fermentation, drinks and flavouring brewage and the fermentation process of livestock liquid dung agriculturally, stalk etc. etc. is all multi-party
Face.
Although combined exemplary embodiment and accompanying drawing describe the present invention, those of ordinary skill in the art above
It will be apparent to the skilled artisan that in the case where not departing from spirit and scope by the claims, various modifications can be carried out to above-described embodiment.
Claims (10)
1. a kind of ultrasonic fermentation reactor, it is characterised in that the ultrasonic fermentation reactor includes tank body, ultrasonic wave occurs
Device and electromagnetic pulse reactor array, wherein, the tank body is provided with material inlet, material outlet, gas vent and water body and gone out
Mouthful, the material inlet can will treat that fermentation materials are delivered in tank body, and the material outlet can arrange the material after fermentation
Go out tank body, the gas vent can discharge caused gas during material fermentation tank body, and the water body outlet can incite somebody to action
Caused water discharge tank body during material fermentation;The supersonic generator is arranged on outside tank body or in tank body, with material
In fermentation process ultrasonic wave is produced into the tank body;The electromagnetic pulse reactor array is by multiple electromagnetic pulse reactor structures
Into and be arranged in tank body, with during material fermentation the tank interior produce electromagnetic pulse.
2. ultrasonic fermentation reactor according to claim 1, it is characterised in that the ultrasonic fermentation reactor also wraps
Mixing component is included, the mixing component can be stirred to the material in ultrasonic fermentation reactor.
3. ultrasonic fermentation reactor according to claim 2, it is characterised in that the mixing component includes being arranged on tank
The reducing gear of percussion mechanism in body, the motor being arranged on outside tank body and the connection percussion mechanism and the motor.
4. ultrasonic fermentation reactor according to claim 3, it is characterised in that the percussion mechanism is included along Vertical Square
To the vibrations main shaft and one or more vibrations arms with main shaft interconnection being arranged in tank body.
5. ultrasonic fermentation reactor according to claim 4, it is characterised in that the multiple electromagnetic pulse reactor is equal
It is even to be arranged on one or more of vibrations arms.
6. ultrasonic fermentation reactor according to claim 1, it is characterised in that the ultrasonic fermentation reactor also wraps
Temperature-controlling component is included, the temperature-controlling component can control fermentation temperature.
7. ultrasonic fermentation reactor according to claim 6, it is characterised in that the temperature-controlling component includes temperature adjustment
Component, feedback computing unit and one or more temperature sensors, wherein, the temperature adjusting member be arranged on tank interior or
Outside tank body, and the material in tank body can be heated or cooled;One or more of temperature sensors are arranged on tank
Temperature of charge can be detected in vivo and in real time;The feedback computing unit detects according to one or more of temperature sensors
To the comparison of temperature value and biofermentation target temperature heated or cooled adjusting the temperature adjusting member.
8. ultrasonic fermentation reactor according to claim 1, it is characterised in that the ultrasonic fermentation reactor also wraps
Tank body control climate unit is included, the tank body control climate unit can control the atmosphere in the tank body to form.
9. ultrasonic fermentation reactor according to claim 1, it is characterised in that the material inlet is arranged on the tank
The top of body, the material outlet are arranged on the bottom of the tank body, and the gas vent is arranged on the top of the tank body, institute
State the bottom that water body outlet is arranged on the tank body.
10. ultrasonic fermentation reactor according to claim 1, it is characterised in that the ultrasonic fermentation reactor is also
Including the electromagnetic pulse insulation blocking cover being arranged on outside the tank body.
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CN108620419A (en) * | 2018-06-15 | 2018-10-09 | 南开大学 | A kind of organic waste low-temperature in-site degradation reaction device and application |
CN112898060A (en) * | 2021-02-07 | 2021-06-04 | 张量 | Processing technology for fermenting human manure into organic fertilizer by using microorganisms |
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Publication number | Priority date | Publication date | Assignee | Title |
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CN108620419A (en) * | 2018-06-15 | 2018-10-09 | 南开大学 | A kind of organic waste low-temperature in-site degradation reaction device and application |
CN108620419B (en) * | 2018-06-15 | 2023-05-09 | 南开大学 | Organic waste low-temperature in-situ degradation reaction device and application |
CN112898060A (en) * | 2021-02-07 | 2021-06-04 | 张量 | Processing technology for fermenting human manure into organic fertilizer by using microorganisms |
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Application publication date: 20180323 |