CN107802716A - A kind of antimicrobial extract of water chestnut plant and its preparation method and application - Google Patents
A kind of antimicrobial extract of water chestnut plant and its preparation method and application Download PDFInfo
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- CN107802716A CN107802716A CN201711269565.XA CN201711269565A CN107802716A CN 107802716 A CN107802716 A CN 107802716A CN 201711269565 A CN201711269565 A CN 201711269565A CN 107802716 A CN107802716 A CN 107802716A
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/89—Cyperaceae (Sedge family)
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/33—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones
- A61K2236/333—Extraction of the material involving extraction with hydrophilic solvents, e.g. lower alcohols, esters or ketones using mixed solvents, e.g. 70% EtOH
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/30—Extraction of the material
- A61K2236/37—Extraction at elevated pressure or temperature, e.g. pressurized solvent extraction [PSE], supercritical carbon dioxide extraction or subcritical water extraction
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/51—Concentration or drying of the extract, e.g. Lyophilisation, freeze-drying or spray-drying
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2236/00—Isolation or extraction methods of medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicine
- A61K2236/50—Methods involving additional extraction steps
- A61K2236/53—Liquid-solid separation, e.g. centrifugation, sedimentation or crystallization
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Abstract
The invention discloses a kind of antimicrobial extract of water chestnut plant and its preparation method and application, preparation method comprises the following steps：(1) dried water chestnut plant is crushed, be placed in subcritical abstraction kettle, subcritical abstraction is carried out by extractant of methanol solution；(2) slag liquid obtained by step (1) is centrifuged, collects supernatant；(3) supernatant obtained by step (2) is placed in the reactor of molecular distillation instrument, carries out molecular distillation, collected cut, obtain the antimicrobial extract of water chestnut plant.This method preparation technology is simple, recovery rate is high, and the antimicrobial extract antimicrobial acivity of water chestnut plant of gained is good, and certain basis has been established for application of the water chestnut in antimicrobial agents research.
Description
Technical field
The present invention relates to belong to pharmaceutical technology field, and in particular to a kind of antimicrobial extract of water chestnut plant and its preparation
Methods and applications.
Background technology
Water chestnut (Eleochairis toberosa), also known as horseshoe (Water chestnut), are that Cyperaceae Eleocharis is more
The underground bulb of year aquatic herbaceous plant water chestnut, there is cultivation each province on the south China Yangtze river basin.Not only how sweet juice is for horseshoe, battalion
Support and enrich, and have good medical value, the good reputation for having " underground pyrus nivalis " and " south of the River ginseng " from ancient times.Traditional Chinese medicine is recognized
For horseshoe is cold in nature sweet, there is heat-clearing, resolving sputum, disperse accumulations and other effects, can treat yellow subcutaneous ulcer, heat gonorrhea, mass in the abdomen, hot eyes, abscess of throat etc.
Disease, it is good merchantable brand in medicine.《Dictionary of medicinal plant》Record, the active component of horseshoe is enriched in the portion between the pericarp of horseshoe and pulp
Position.
But so far, never reported the anti-microbial effect correlative study of water chestnut.Microorganism drug resistance has turned into
The Tough questions of global concern, the infection as caused by multi-drug resistant bacteria and many new pathogenic microorganisms cause pole to human health
It is big to threaten.With the extensive use of antimicrobial agents, hospital infection microorganism drug resistance problems are on the rise, fungal infection
Clinically also there is ascendant trend year by year.The drug resistance of microorganism produces speed, is far longer than antibiotic and develops speed.Therefore,
It is the task of top priority to prevent that microorganism drug resistance from producing and finding specific antibacterial medicine.
The content of the invention
The technical problem to be solved in the present invention is overcome the deficiencies in the prior art, there is provided a kind of preparation technology is simple, extraction
The preparation method of the high antimicrobial extract of water chestnut plant of rate, the antimicrobial work of the antimicrobial extract of water chestnut plant of gained
Property it is good, for water chestnut antimicrobial agents research in application established certain basis.
In order to solve the above technical problems, the present invention uses following technical scheme：
A kind of preparation method of the antimicrobial extract of water chestnut plant, comprises the following steps：
(1) dried water chestnut plant is crushed, be placed in subcritical abstraction kettle, carried out by extractant of methanol solution
Extraction；
(2) slag liquid obtained by step (1) is centrifuged, collects supernatant；
(3) supernatant obtained by step (2) is placed in the reactor of molecular distillation instrument, carries out molecular distillation, collection evaporates
Point, obtain the antimicrobial extract of water chestnut plant.
Preferably, in the step (1), extracting pressure is 25MPa~50MPa, and extraction temperature is 120 DEG C~150 DEG C.
Preferably, in the step (1), the speed of agitator during subcritical abstraction is 150rpm~200rpm, extraction
Time is 5min~10min；The subcritical abstraction process is carried out in closed container, under nitrogen protection.
Preferably, in the step (1), the weight/mass percentage composition of the methanol solution is 55%~95%, the methanol
Solution and the amount ratio of water chestnut plant are 10mL~12mL: 1g.
Preferably, in the step (2), the centrifugation is to carry out at normal temperatures, the speed of the centrifugation for 4000 × g~
6000 × g, the time of the centrifugation is 10min~15min.
Preferably, in the step (3), the molecular distillation temperature be 80 DEG C~90 DEG C, distillation pressure be 200Pa~
250Pa, charging rate are 2.0mL/min~3.0mL/min, and rotating speed is 250r/min~350r/min.
The inventive concept total as one, the present invention also provide a kind of antimicrobial extract of water chestnut plant, the water chestnut
The antimicrobial extract of plant is as obtained by above-mentioned preparation method.
The inventive concept total as one, the water chestnut plant that the present invention is also provided obtained by a kind of above-mentioned preparation method resist
Application of the microorganism extracts in antimicrobial is prepared.
Compared with prior art, the advantage of the invention is that：
1st, it is good to have found that water chestnut plant extract has upon the look during herbal medicine antimicrobial acivity by the present inventor
Antimicrobial acivity.This is that the later stage antimicrobial or auxiliary therapeutic action research of this Plant Extracts has been established necessarily
Basis.
2nd, extracting method of the invention is simple and practical, and for Subcritical water chromotagraphy using water as Extraction solvent, this method can make water chestnut
The antimicrobial efficient dissolution of active ingredient in plant cell, then gone out antimicrobial extracts active ingredients with molecular distillation technique
Come, cost is greatlyd save while production efficiency is improved, and greatly reduce the pollution to environment, there is good application
Prospect and economic benefit.
3rd, water chestnut plant extract produced by the present invention has good inhibiting effect to microorganism；Experimental result is shown：
95% methanol water chestnut sub-critical extraction thing is best to the inhibitory activity of microorganism, and is in dose-dependant to the inhibiting rate of microorganism
Property.
Embodiment
Below in conjunction with specific preferred embodiment, the invention will be further described, but not thereby limiting the invention
Protection domain.
Embodiment 1：
A kind of preparation method of the antimicrobial extract of water chestnut plant of the invention, comprises the following steps：
(1) dried water chestnut plant (rhizome and branches and leaves part) is crushed, is placed in subcritical abstraction kettle, adds matter
It is extractant to measure the methanol solution that percentage composition is 95%, wherein, methanol solution and the amount ratio of water chestnut plant are 10mL:
1g, subcritical abstraction 10min is carried out in the case where pressure is 25MPa, temperature is 150 DEG C, speed of agitator 200rpm, nitrogen are protected；
(2) slag liquid obtained by step (1) is carried out centrifuging 15min in the case where normal temperature, rotating speed is 4000 × g, collects supernatant；
(3) supernatant obtained by step (2) is placed in the reactor of molecular distillation instrument, is 80 DEG C in temperature, distillation pressure
Power is 250Pa, and charging rate 2.0mL/min, rotating speed is that molecular distillation is carried out under 250r/min, collects cut, obtains water chestnut
The antimicrobial extract of plant.
Embodiment 2：
A kind of preparation method of the antimicrobial extract of water chestnut plant of the invention, comprises the following steps：
(1) dried water chestnut plant (rhizome and branches and leaves part) is crushed, is placed in subcritical abstraction kettle, adds matter
It is extractant to measure the methanol solution that percentage composition is 75%, wherein, methanol solution and the amount ratio of water chestnut plant are 11mL:
1g, subcritical abstraction 8min is carried out in the case where pressure is 40MPa, temperature is 130 DEG C, speed of agitator 200rpm, nitrogen are protected；
(2) slag liquid obtained by step (1) is carried out centrifuging 12min in the case where normal temperature, rotating speed is 5000 × g, collects supernatant；
(3) supernatant obtained by step (2) is placed in the reactor of molecular distillation instrument, is 85 DEG C in temperature, distillation pressure
Power is 220Pa, and charging rate 2.5mL/min, rotating speed is that molecular distillation is carried out under 300r/min, collects cut, obtains water chestnut
The antimicrobial extract of plant.
Embodiment 3：
A kind of preparation method of the antimicrobial extract of water chestnut plant of the invention, comprises the following steps：
(1) dried water chestnut plant (rhizome and branches and leaves part) is crushed, is placed in subcritical abstraction kettle, adds matter
It is extractant to measure the methanol solution that percentage composition is 55%, wherein, methanol solution and the amount ratio of water chestnut plant are 12mL:
1g, subcritical abstraction 8min is carried out in the case where pressure is 50MPa, temperature is 120 DEG C, speed of agitator 150rpm, nitrogen are protected；
(2) slag liquid obtained by step (1) is carried out centrifuging 10min in the case where normal temperature, rotating speed is 6000 × g, collects supernatant；
(3) supernatant obtained by step (2) is placed in the reactor of molecular distillation instrument, is 90 DEG C in temperature, distillation pressure
Power is 200Pa, and charging rate 3.0mL/min, rotating speed is that molecular distillation is carried out under 350r/min, collects cut, obtains water chestnut
The antimicrobial extract of plant.
Embodiment 4：Inhibitory activity of the water chestnut extract to microorganism
1. reagent：Fluconazole (Fluconazole) and chloramphenicol (Chloroamphenicol)：Shanghai three-dimensional pharmacy is limited
Company, lot number are respectively 20151109 and 20160717；Bacterium bacterial strain：Staphylococcus aureus (ATCC 25923,
Sa), Bacillus sublitis (ATCC 6633, Bs) and Escherichia coli (ATCC 25922, Ec) therefrom traditional Chinese medicines
Product biological products assay institute is bought；Fungal bacterial strain：Candida albicans (ATCC 64550, Ca), C.krusei (ATCC
62588, Ck) and C.parapsilosis (ATCC 22019, Cp) is bought from Nat'l Pharmaceutical ＆ Biological Products Control Institute.
2. method of testing：Antibacterial activity test is tested using the broth dilution method of document description, selects methanol extraction
Thing is testing sample.Mother liquor is prepared using dimethyl sulfoxide, is configured to 2.0mg/mL concentration, and mother liquor uses two times of method gradients
It is diluted to 6 concentration gradients.Concrete operations are as follows：0.5mL dilution is added to containing in test tubes of the 4.4mL containing dilute meat soup, is connect
The strain suspension for addition 0.1mL is well mixed, and the volume of whole nutrient solution is 5.0mL.The sample concentration scope of test is
The test concentrations scope of 6.25 to 200 μ g/mL, positive control medicine chloramphenicol and Fluconazole be respectively 2.0-265 μ g/mL and
1.56-256μg/mL.24h, test and the minimal inhibitory concentration (MIC) for calculating sample are cultivated at 37 DEG C.
The water chestnut plant extract of various concentrations methanol extraction is as described in Table 1 to the inhibitory activity result of microorganism：
The water chestnut plant extract of the various concentrations methanol extraction of table 1. is to Antimicrobial activity
Wherein, positive control is used as using Fluconazole and chloramphenicol.
As it can be seen from table 1 the water chestnut plant extract of methanol extraction has good inhibiting effect, wherein matter to microorganism
It is most strong for the bacteriostatic activity of 95% methanolic extract to measure percentage composition.
It is described above, only it is the preferred embodiment of the application, any type of limitation is not done to the application, although this Shen
Please with preferred embodiment disclose as above, but and be not used to limit the application, any person skilled in the art, do not taking off
In the range of technical scheme, make a little variation using the technology contents of the disclosure above or modification is equal to
Case study on implementation is imitated, is belonged in the range of technical scheme.
Claims (8)
1. a kind of preparation method of the antimicrobial extract of water chestnut plant, comprises the following steps：
(1) dried water chestnut plant is crushed, be placed in subcritical abstraction kettle, carrying out Asia as extractant using methanol solution faces
Boundary extracts；
(2) slag liquid obtained by step (1) is centrifuged, collects supernatant；
(3) supernatant obtained by step (2) is placed in the reactor of molecular distillation instrument, carries out molecular distillation, collected cut, obtain
To the antimicrobial extract of water chestnut plant.
2. the preparation method of the antimicrobial extract of water chestnut plant according to claim 1, it is characterised in that the step
(1) in, extracting pressure is 25MPa~50MPa, and extraction temperature is 120 DEG C~150 DEG C.
3. the preparation method of the antimicrobial extract of water chestnut plant according to claim 2, it is characterised in that the step
(1) in, the speed of agitator during subcritical abstraction is 150rpm~200rpm, and extraction time is 5min~10min；The Asia
Critical extraction process is carried out in closed container, under nitrogen protection.
4. the preparation method of the antimicrobial extract of water chestnut plant according to claim 3, it is characterised in that the step
(1) in, the weight/mass percentage composition of the methanol solution is 55%~95%, and the methanol solution and the amount ratio of water chestnut plant are
10mL~12mL: 1g.
5. the preparation method of the antimicrobial extract of water chestnut plant according to any one of Claims 1 to 4, its feature exist
In in the step (2), the centrifugation is to carry out at normal temperatures, and the speed of the centrifugation is 4000 × g~6000 × g, described
The time of centrifugation is 10min~15min.
6. the preparation method of the antimicrobial extract of water chestnut plant according to any one of Claims 1 to 4, its feature exist
In in the step (3), the molecular distillation temperature is 80 DEG C~90 DEG C, and distillation pressure is 200Pa~250Pa, charging rate
For 2.0mL/min~3.0mL/min, rotating speed is 250r/min~350r/min.
7. a kind of antimicrobial extract of water chestnut plant, it is characterised in that the antimicrobial extract of water chestnut plant is by right
It is required that obtained by preparation method described in 1~6 any one.
8. the antimicrobial extract of water chestnut plant obtained by a kind of preparation method as described in any one of claim 1~6 exists
Prepare the application in antimicrobial.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201711269565.XA CN107802716A (en) | 2017-12-05 | 2017-12-05 | A kind of antimicrobial extract of water chestnut plant and its preparation method and application |
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| CN201711269565.XA CN107802716A (en) | 2017-12-05 | 2017-12-05 | A kind of antimicrobial extract of water chestnut plant and its preparation method and application |
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Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103980291A (en) * | 2014-04-29 | 2014-08-13 | 天津大学 | Method for extracting pterygsspermine from moringa oleifera lam roots |
| CN104530069A (en) * | 2014-12-29 | 2015-04-22 | 贺州学院 | Method for extracting puchiin B from eleocharis tuberosa peel |
-
2017
- 2017-12-05 CN CN201711269565.XA patent/CN107802716A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103980291A (en) * | 2014-04-29 | 2014-08-13 | 天津大学 | Method for extracting pterygsspermine from moringa oleifera lam roots |
| CN104530069A (en) * | 2014-12-29 | 2015-04-22 | 贺州学院 | Method for extracting puchiin B from eleocharis tuberosa peel |
Non-Patent Citations (1)
| Title |
|---|
| 李冬生等: "荸荠叶茎提取物抑菌作用的研究", 《粮油加工与食品机械》 * |
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Application publication date: 20180316 |